Emulsion composition with multiple moisturizing effects

Abstract

The invention discloses an emulsified composition with multiple moisturizing effects, which is prepared from the following components in percentage by weight: 0.8 to 2 percent of polyglycerol-4 stearate, 1 to 3 percent of C12-20 alkyl glucoside, C14-22 alcohol and water, and the weight ratio of the polyglycerol-4 stearate to the C12-20 alkyl glucoside to the C14-22 alcohol is (1 to 1.6) to (2.3 to 1.7). The invention also relates to an emulsion composition with multiple moisturizing effects, which can be applied to the field of personal care products.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular to a preparation method of an emulsified composition with multiple moisturizing effects and application thereof. BACKGROUND

[0002] With the steady development of China's social economy, people's quality and requirements for daily life are getting higher and higher. As a product for improving life happiness, cosmetics are more and more favored by consumers.

[0003] Traditional cosmetics usually use raw materials with emulsifying effect to combine oil and water in the formula. This step is commonly known as "emulsification". Emulsification can "integrate" oil and water that were originally separated into a white-looking emulsion. Such raw materials are usually called emulsifiers.

[0004] Among the many emulsifier raw materials, polyglycerol fatty acid esters, as a new type of emulsifier, have the advantages of natural, high safety, wide HLB value range, good emulsification stability, unique skin feel, etc. and are widely used in food, daily chemical, medicine and other fields. Another emulsifier is a raw material with emulsifying effect composed of alkyl glycoside and high-grade fatty alcohol. It is natural and mild without irritation, and is also widely used in various fields.

[0005] However, in actual application, it is found that emulsifiers can help products complete emulsification, but they usually have some negative effects, such as sticky skin feel, impurities and irritants, etc., which affect the skin feel and safety of the product. Considering the prevalence of children's and infant's cosmetics in recent years, the use of natural source emulsifiers for product formulation design can better meet the current people's expectations for green, environmentally friendly and safe personal care products. Further, if the natural source emulsification system also has other effects such as moisturizing, the addition of corresponding functional ingredients in the formula can be effectively reduced, and the green concept of pure formula can be better met.

[0006] Some past technical solutions have also disclosed some application means of natural emulsified compositions. However, these technical solutions mostly describe the role of natural source emulsifiers in stabilizing the emulsification system, and do not study the related effects of the emulsified composition. For example, Chinese patent application CN202311074080.0 discloses a stable natural emulsified composition, its preparation method and application, which does not provide related research data on the effects such as moisturizing of the emulsification system. Compared with only studying the stabilizing effect of natural emulsified composition on the emulsification system, it is more difficult to study and find a natural emulsified composition that can not only stabilize the emulsification system but also bring additional effects.

[0007] Among all product efficacies, moisturizing efficacy as the most basic and important efficacy is applied in various personal care products. Therefore, it has important practical significance to research and develop a natural emulsified composition which can stabilize the emulsified system and bring moisturizing efficacy. SUMMARY

[0008] In one aspect, the present application provides an emulsified composition with multiple moisturizing effects, comprising: 0.8-2 wt% of polyglyceryl-4 stearate, 1-3 wt% of C12-20 alkyl glucoside and C14-22 alcohol and water, and the weight ratio of polyglyceryl-4 stearate to C12-20 alkyl glucoside and C14-22 alcohol is 1-1.6:2.3-1.7.

[0009] In a preferred embodiment, the composition comprises 0.8-1.3 wt% of polyglyceryl-4 stearate.

[0010] In a preferred embodiment, the composition comprises 0.827-1.3 wt% of polyglyceryl-4 stearate.

[0011] In a preferred embodiment, the composition comprises 1.2-2 wt% of C12-20 alkyl glucoside and C14-22 alcohol.

[0012] In a preferred embodiment, the solid content of the composition is 2.1-3.3 wt%.

[0013] In a preferred embodiment, the FLG up-regulation rate of the composition is 46-94%, the LOR up-regulation rate is 30-145%, the AQP-3 up-regulation rate is 46-115%, and the ZO-1 up-regulation rate is 52-105%.

[0014] In a preferred embodiment, the composition can be applied to personal care products.

[0015] In a preferred embodiment, the added amount of the composition in the personal care product is 2.1-3.3 wt%.

[0016] In a preferred embodiment, the personal care product is a product in the form of infant emollient cream, infant body milk, infant hand cream, adult cream, adult eye cream, etc. DETAILED DESCRIPTION

[0017] For the sake of providing a more concise description, some numerical expressions given herein are not modified by the term "about". It should be understood that, whether or not the term "about" is explicitly used, each quantity given herein is intended to refer to both an actual given value and also to an approximate value close to the actual given value, as would be reasonably ascertained by one of ordinary skill in the art, including a value close to the actual given value that is caused by experimental and / or measurement conditions.

[0018] Solids content (wt%) The solid content is the content of the components in the composition excluding water. For example, the solid content of Example 1 is the ratio of the added amount of polyglyceryl-4 stearate to the total weight of the composition. For another example, the solid content of Example 4 is the ratio of the added amount of C12-20 alkyl glucoside and C14-22 alcohol to the total weight of the composition.

[0019] Examples The present application is further described in the following specific examples, which do not limit the present application in any manner, but merely serve to illustrate the present application, and any modifications of the present application made within the scope of the claims of the present application are still within the scope of the present application.

[0020] Unless otherwise indicated, all percentages and parts are by weight. Unless otherwise defined, all other technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the application belongs. In addition, any method and material similar or equivalent to those described herein can be used in the practice of the present application. The particular methods and materials described herein are exemplary and are not intended to limit the scope of the application.

[0021] Experimental materials: Polyglyceryl-4 stearate, purchased from DOW Chemical; C12-20 alkyl glucoside and C14-22 alcohol, purchased from SEPPIC 3D epidermal skin model EpiKutis®, purchased from Guangdong Boxi Biological; EpiGrowth culture solution, purchased from Guangdong Boxi Biological; PBS, purchased from Solabio; WY14643 (Pioglitazone), purchased from Sigma; CaCl2, purchased from National Pharmaceutical; RNAiso Plus, purchased from Aikcolor Biological; Reverse transcription kit, purchased from Aikcolor Biological; Fluorescent dye, purchased from Aikcolor Biological.

[0022] CO2 incubator, model 150I, purchased from Thermo; Super-clean workbench, model SW-CJ-1F, purchased from Suzhou Antai; General PCR instrument, purchased from Bio-Rad; Fluorescent quantitative PCR instrument, model CFX-96, purchased from BioRad; Skin moisture content tester, model Corneometer CM 825, purchased from Courage+Khazaka electronic.

[0023] Example 1: Sample Preparation Respectively weigh 3g of Polyglyceryl-4 Stearate into 97g of deionized water, then put into water bath at 80°C until the sample is in a homogeneous suspension state.

[0024] Example 2: Sample Preparation Respectively weigh 4g of Polyglyceryl-4 Stearate into 96g of deionized water, then put into water bath at 80°C until the sample is in a homogeneous suspension state.

[0025] Example 3: Sample Preparation Respectively weigh 5g of Polyglyceryl-4 Stearate into 95g of deionized water, then put into water bath at 80°C until the sample is in a homogeneous suspension state.

[0026] Example 4: Sample Preparation Respectively weigh 3g of C12-20 Alkyl Glucoside and C14-22 Alcohol into 97g of deionized water, then put into water bath at 80°C until the sample is in a homogeneous suspension state.

[0027] Example 5: Sample Preparation Respectively weigh 4g of C12-20 Alkyl Glucoside and C14-22 Alcohol into 96g of deionized water, then put into water bath at 80°C until the sample is in a homogeneous suspension state.

[0028] Example 6: Sample Preparation Respectively weigh 5g of C12-20 Alkyl Glucoside and C14-22 Alcohol into 95g of deionized water, then put into water bath at 80°C until the sample is in a homogeneous suspension state.

[0029] Examples 1-6 are the preparation of the test compositions at 3%, 4%, 5% solid content respectively Respectively weigh 3g, 4g, 5g of Polyglyceryl-4 Stearate and C12-20 Alkyl Glucoside and C14-22 Alcohol into 97g, 96g, 95g of deionized water, then put into water bath at 80°C until the sample is in a homogeneous suspension state, as shown in Table 1 below.

[0030] Table 1 Test Example 1: Skin Moisture Content Test (Human) Test Sample: Samples prepared in Examples 1-6.

[0031] Subjects: 5 healthy subjects aged 18-60 years old, male and female.

[0032] Test Method: This test is a randomized, double-blind, forearm medial test design. 5 qualified subjects are selected to participate in this test. Test samples and blank controls are applied to the subjects' forearm medial, with an area of (3.0 x 4.0) cm for each region 2 The staff applies the test samples to the test area on the subjects' forearm medial, with a dosage of (2.00 ± 0.05) mg / cm for each product 2 Before the product is used (initial value), immediately after the product is used once, the skin moisture content test is performed.

[0033] Table 2 Test Example 1 investigates the moisturizing effect of both at a solid content of 3%, 4%, and 5% concentration, and the results are shown in Table 2. From the results, it can be seen that the polyglyceryl-4 stearate aqueous solution prepared in Examples 1-3, and the aqueous solution samples of C12-20 alkyl glucoside and C14-22 alcohol prepared in Examples 4-6, both have certain moisturizing efficacy at 3%, 4%, and 5% concentrations, respectively.

[0034] However, for actual formulation applications, the emulsification efficiency of a single emulsifier is much lower than that of a combination of the two. If the moisturizing efficacy can also be significantly improved after the two are combined, then this natural source emulsification composition has a more excellent effect. The following is the preparation and testing of the combined samples.

[0035] Example 7: Sample Preparation 1 g of polyglyceryl-4 stearate and 2.3 g of C12-20 alkyl glucoside and C14-22 alcohol were weighed separately and added to 96.7 g of deionized water, and then placed in a water bath at 80°C until the sample was in a homogeneous suspension state.

[0036] Example 8: Sample Preparation 1.3 g of polyglyceryl-4 stearate and 2 g of C12-20 alkyl glucoside and C14-22 alcohol were weighed separately and added to 96.7 g of deionized water, and then placed in a water bath at 80°C until the sample was in a homogeneous suspension state.

[0037] Example 9: Sample Preparation 1.6 g of polyglyceryl-4 stearate and 1.7 g of C12-20 alkyl glucoside and C14-22 alcohol were weighed separately and added to 96.7 g of deionized water, and then placed in a water bath at 80°C until the sample was in a homogeneous suspension state.

[0038] Examples 7-9 were prepared with a total solid content of 3.3% at a ratio of 1:2.3, 1.3:2, and 1.6:1.7, respectively, as shown in Table 3 below.

[0039] Table 3 Test Example 2: Effect on Moisture Factor FLG Test Sample: Composition configured in Examples 7-9 Four common moisture factors FLG were selected for the moisturizing efficacy test of the composition.

[0040] In advance, 0.9 mL of Epigrowth culture solution was added to a 6-well plate, the BC group (blank control) was not treated, the PC group (positive control: WY-14643 pirlinica) was added with the corresponding concentration of positive control sample, and the sample group was added with the corresponding concentration of the sample to be tested, and was incubated in a CO2 incubator (37°C, 5% CO2) for 24 h. After incubation, the model ring was cut off and placed in a 5 mL centrifuge tube, and 1 mL of RNAiso Plus was added. After extraction of RNA and reverse transcription to cDNA, fluorescence quantitative PCR detection was performed, and the results were calculated by 2-△△CT method.

[0041] Up-regulation rate (%) = (test sample group - blank control group) / blank control group * 100%.

[0042] The effect of the samples configured in Examples 7-9 on the moisture factor FLG was investigated according to the aforementioned moisture factor test method when the total solid content was 3.3% by weight, the ratio of polyglyceryl-4 stearate to C12-20 alkyl glucoside and C14-22 alcohol was 1:2.3, 1.3:2, and 1.6:1.7, respectively, and the results are shown in Table 4.

[0043] Table 4 Promotion of Moisture Factor FLG Expression FLG, i.e., filaggrin, is an important molecule that connects keratin fibers in the stratum corneum of human skin. Under the assistance of FLG monomers, keratin fibers are regularly aggregated to form a solid physical barrier on the outermost layer of the epidermis, which can prevent the loss of epidermal water and the invasion of external allergens. Therefore, if the FLG gene content can be increased, the moisturizing effect can be effectively improved.

[0044] As can be seen from the results, Example 8 has the best effect on promoting the expression of moisture factor FLG among the three concentration ratios. Further tests were conducted on other moisture factors, and the results were observed.

[0045] Test Example 3: Effect on Moisture Factor LOR Test sample: compositions configured in Examples 7-9 The four common moisturizing factors LOR were selected for the moisturizing efficacy test of the compositions.

[0046] Add 0.9 mL of Epigrowth culture solution in advance in a 6-well plate, do not treat the BC group (blank control), add the corresponding concentration of positive control sample to the PC group (positive control: WY-14643 pioglitazone), add the corresponding concentration of test sample to the sample group, and incubate in a CO2 incubator (37°C, 5% CO2) for 24 h. After incubation, cut off the model ring, place it in a 5 mL centrifuge tube, and add 1 mL of RNAiso Plus. Extract RNA, reverse transcribe to cDNA, and then perform fluorescence quantitative PCR detection. The results are calculated using the 2-△△CT method.

[0047] Up-regulation rate (%) = (test sample group - blank control group) / blank control group * 100%.

[0048] The effects of the samples configured in Examples 7-9 on the moisturizing factor LOR were investigated according to the aforementioned moisturizing factor test method when the total solid content was 3.3% by weight, the ratio of polyglyceryl-4 stearate to C12-20 alkyl glucoside and C14-22 alcohol was 1:2.3, 1.3:2, and 1.6:1.7, respectively. The results are shown in Table 5.

[0049] Table 5 LOR, i.e., filaggrin, is an important component involved in skin barrier formation. It is the main component of keratinocyte envelope, accounting for about 80% of the total keratinocyte envelope protein, and mainly exists in the epidermal granular layer, playing an important role in the barrier function of the epidermis. If the LOR gene content can be improved, the barrier function can be effectively protected, and problems such as dry skin can be reduced.

[0050] According to the results, Example 8 has the best effect on promoting the expression of the moisturizing factor LOR among the three concentration ratios. Continue to test other moisturizing factors and observe the results.

[0051] Test Example 4: Effect on Moisturizing Factor AQP-3 Test sample: compositions configured in Examples 7-9 The four common moisturizing factors AQP-3 were selected for the moisturizing efficacy test of the compositions.

[0052] In 6-hole plates, 0.9 mL of Epigrowth culture solution was added in advance, the BC group (blank control) was not treated, the PC group (positive control: CaCl2) was added with the corresponding concentration of positive control sample, and the sample group was added with the corresponding concentration of sample to be tested, and was placed in a CO2 incubator (37°C, 5% CO2) for incubation for 24 h. After incubation, the model ring was cut off and placed in a 5 mL centrifuge tube, and 1 mL of RNAiso Plus was added. After extracting RNA and reverse transcribing to cDNA, fluorescence quantitative PCR detection was performed, and the results were calculated by 2-△△CT method.

[0053] Up-regulation rate (%) = (test sample group - blank control group) / blank control group * 100%.

[0054] The total solid content of the sample configured in Example 7-9 was 3.3% by weight, and the ratio of polyglyceryl-4 stearate to C12-20 alkyl glucoside and C14-22 alcohol was 1:2.3, 1.3:2, and 1.6:1.7, respectively. The effect of the sample on the moisturizing factor AQP-3 was investigated according to the aforementioned moisturizing factor test method, and the results are shown in Table 6.

[0055] Table 6 AQP-3, also known as water channel protein 3, is a protein located on the cell membrane (intrinsic membrane protein) that forms a "pore" on the cell membrane and can control the entry and exit of water in the cell, like a "cell water pump". It has the function of transporting small molecular substances such as water, glycerol and urea across the membrane. If the content of AQP3 gene can be increased, the water content of the skin can be significantly increased, and the moisturizing effect can be promoted.

[0056] From the results, it can be seen that Example 8 has the best effect on promoting the expression of moisturizing factor AQP-3 among the three concentration ratios. Continue to test other moisturizing factors and observe the results.

[0057] Test Example 5: Effect on Moisturizing Factor ZO-1 Test sample: composition configured in Examples 7-9 This test selects four common moisturizing factors ZO-1 to test the moisturizing effect of the composition.

[0058] In the 6-well plate, 0.9 mL of Epigrowth culture solution was added in advance, the BC group (blank control) was not treated, the PC group (positive control: CaCl2) was added with the corresponding concentration of positive control sample, and the sample group was added with the corresponding concentration of the sample to be tested, and was incubated in a CO2 incubator (37°C, 5% CO2) for 24 h. After incubation, the model ring was cut off and placed in a 5 mL centrifuge tube, and 1 mL of RNAiso Plus was added. After extracting RNA and reverse transcribing to cDNA, fluorescence quantitative PCR detection was performed, and the results were calculated by the 2-△△CT method.

[0059] Up-regulation rate (%) = (test sample group - blank control group) / blank control group * 100%.

[0060] The total solid content of the sample configured in Examples 7-9 was 3.3% by weight, and the ratio of polyglyceryl-4 stearate to C12-20 alkyl glucoside and C14-22 alcohol was 1:2.3, 1.3:2, and 1.6:1.7, respectively. According to the aforementioned moisturizing factor test method, the effect of the composition on the moisturizing factor ZO-1 was investigated, and the results are shown in Table 7.

[0061] Table 7 ZO-1 is a tight junction protein that mainly exists in the tight junction region of epithelial cells and endothelial cells. It plays a key role in maintaining the integrity of intercellular junctions, regulating cell polarity, and intercellular material exchange. In terms of moisturizing, ZO-1 helps maintain skin barrier function and prevent water loss, thus playing a crucial role in skin moisturizing.

[0062] As can be seen from the results, Example 8 has the best effect on promoting the expression of moisturizing factor ZO-1 among the three concentration ratios.

[0063] As can be seen from the results, Example 8 has the best effect on promoting the expression of moisturizing factor ZO-1 among the three concentration ratios.

[0064] Next, the skin water content of the composition with different ratios was measured by 3D skin model, and the actual moisturizing effect was observed.

[0065] Test Example 6: Skin Water Content Test (3D Skin) Test sample: composition configured in Examples 7-9 In the 6-hole plate, 0.9 mL of EpiGrowth culture solution was added in advance, the BC group (blank control) was not treated, the PC group (positive control: glycerol) was added with the corresponding concentration of positive control sample, and the sample group was added with the corresponding concentration of sample to be tested, and was placed in a CO2 incubator (37°C, 5% CO2) for incubation for 24 h. After incubation, the surface of the model was washed with sterile PBS solution to remove residual test substances, and the residual liquid inside and outside the model was wiped off with a sterile cotton swab. According to the number of face models, a 24-hole plate was prepared. 0.3 mL of EpiGrowth culture solution was added to each hole. The 24-hole plate containing the model was placed in a clean bench, the cover of the 24-hole plate was opened, and after standing for 30 min, the measurement was performed. The bottom of the model was wiped dry, the model was cut off, and placed in the probe position of the tester. The probe was pressed to measure three times for each model, and the average value was obtained.

[0066] Lifting rate (%) = (sample group - blank control group) / blank control group * 100%.

[0067] The total solid content of the sample prepared in Example 7-9 was 3.3% by weight, and the ratio of polyglyceryl-4 stearate to C12-20 alkyl glucoside and C14-22 alcohol was 1:2.3, 1.3:2, and 1.6:1.7, respectively. The actual moisturizing effect was investigated according to the aforementioned skin water content (3D skin) test method, and the results are shown in Table 8.

[0068] Table 8 As can be seen from the results, Example 8 has the best effect on skin water content in the three concentration ratios, with a skin water content lifting rate of 355.3%, and outstanding effect. Based on the raw material ratio of Example 8, the skin water content (3D skin) test verification will be carried out by adjusting the solid content.

[0069] Example 10: Sample preparation 0.591 g of polyglyceryl-4 stearate and 0.909 g of C12-20 alkyl glucoside and C14-22 alcohol were weighed respectively and added to 98.5 g of deionized water, then placed in a water bath at 80°C until the sample was in a homogeneous suspension state.

[0070] Example 11: Sample preparation 0.827 g of polyglyceryl-4 stearate and 1.273 g of C12-20 alkyl glucoside and C14-22 alcohol were weighed respectively and added to 97.9 g of deionized water, then placed in a water bath at 80°C until the sample was in a homogeneous suspension state.

[0071] Example 12: Sample preparation Take 1.064 g of polyglyceryl-4 stearate and 1.636 g of C12-20 alkyl glucoside and C14-22 alcohol, respectively, and add to 97.3 g of deionized water, and then put into a water bath at 80°C until the sample is in a homogeneous suspension state for use.

[0072] Examples 10-12 are compositions prepared with a ratio of 1.3:2, and total solid contents of 1.5%, 2.1%, and 2.7%, as shown in Table 9 below.

[0073] Table 9 Test Example 7: Skin Moisture Content Test (3D Skin) Test Sample: Compositions configured in Examples 10-12 Add 0.9 mL of EpiGrowth culture solution in advance in a 6-well plate, where the BC group (blank control) is not treated, the PC group (positive control: glycerol) is added with the corresponding concentration of positive control sample, and the sample group is added with the corresponding concentration of the sample to be tested, and placed in a CO2 incubator (37°C, 5% CO2) for incubation for 24 h. After incubation, wash the surface of the model with sterile PBS solution to remove residual test substances, and use a sterile cotton swab to wipe off the residual liquid inside and outside the model. According to the number of face models, prepare a 24-well plate. Add 0.3 mL of EpiGrowth culture solution to each well. Place the 24-well plate containing the models in a clean bench, open the lid of the 24-well plate, and after standing for 30 min, measure. Dry the bottom of the model, cut off the model, and place it in the probe position of the tester, press the probe to measure, measure each model three times, and take the average value.

[0074] Lift rate (%) = (sample group - blank control group) / blank control group * 100%.

[0075] Investigate the samples configured in Examples 10-12, with a fixed ratio of polyglyceryl-4 stearate: C12-20 alkyl glucoside and C14-22 alcohol = 1.3:2, and investigate the moisturizing efficacy of different solid contents at this ratio according to the aforementioned skin moisture content (3D skin) test method, and the results are shown in Table 10.

[0076] Table 10 From the results, it can be seen that when C12-20 alkyl glucoside and C14-22 alcohol = 1.3:2, even if the solid content is reduced to 2.1% (Example 10), its promotion of 3D skin water content is still much higher than that of other ratios under 3.3% solid content (Example 7, Example 9). Therefore, from the perspective of concentration ratio of the two emulsifiers, polyglyceryl-4 stearate: C12-20 alkyl glucoside = 1.3:2 has the best effect, and excellent moisturizing effect can be achieved when the solid content is from 2.1% to 3.3%.

[0077] In summary, compared with the moisturizing ability of a single emulsifier, compounding the two can not only reduce the total solid content, reduce the cost, and improve the safety, but also greatly improve the moisturizing effect. The composition of the present application can promote the expression of multiple moisturizing genes, and is a natural source emulsion composition with multiple moisturizing effects.

[0078] Application Example The composition described in the present application can be used as an intermediate raw material for the preparation of corresponding personal care products, preferably resident products, including but not limited to the preparation of infant emollient cream, body milk, hand cream, and adult face cream, eye cream, etc. The following are specific application examples of the composition obtained by the examples in personal care products, as well as the formula and preparation method of these dosage forms. Specific application examples are as follows: Application Example 1: Preparation of infant emollient cream Application Example 2: Preparation of infant body milk Application Example 3: Preparation of infant hand cream Application Example 4: Preparation of adult face cream Application Example 5: Preparation of adult eye cream

Claims

1. An emulsified composition having multiple moisturizing effects, comprising: 0.8-2% by weight of polyglycerol-4 stearate, 1-3% by weight of C12-20 alkyl glucoside and C14-22 alcohol and water, wherein the weight ratio of polyglycerol-4 stearate to C12-20 alkyl glucoside and C14-22 alcohol is 1-1.6:2.3-1.

7.

2. The composition according to claim 1, characterized in that, The composition contains 0.8-1.3% by weight of polyglycerol-4 stearate.

3. The composition according to claim 1, characterized in that, The composition contains 0.827-1.3% by weight of polyglycerol-4 stearate.

4. The composition according to claim 1, characterized in that, The composition comprises 1.2-2% by weight of C12-20 alkyl glucoside and C14-22 alcohol.

5. The composition according to claim 1, characterized in that, The composition has a solid content of 2.1-3.3% by weight.

6. The composition according to any one of claims 1-5, characterized in that, The composition has an upregulation rate of 46-94% for FLG, 30-145% for LOR, 46-115% for AQP-3, and 52-105% for ZO-1.

7. The composition according to any one of claims 1-5, characterized in that, The composition can be used in personal care products.

8. The composition according to claim 7, characterized in that, The composition is added to personal care products at a rate of 2.1-3.3% by weight.

9. The composition according to claim 7, characterized in that, The personal care products mentioned are products in the form of baby moisturizing cream, baby body lotion, baby hand cream, adult face cream, adult eye cream, etc.

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