Oral cavity lubricating composition, auxiliary agent and preparation method and application of oral cavity lubricating composition and auxiliary agent

Abstract

The invention belongs to the technical field of food functional ingredients and biological materials, and particularly discloses an oral cavity lubrication composition, an additive and a preparation method and application of the oral cavity lubrication composition and the additive. The lubrication enhancer is one or more of sugar alcohol compounds; the interface stabilizer is one or more of mammal-derived mucoproteins. According to the oral cavity lubricating composition, the additive and the preparation method and application of the additive, the preparation process is simple and controllable, the lubricating additive has good biocompatibility, the lubricating effect in the oral cavity is remarkably improved, the oral cavity dryness is relieved, the food oral cavity digestion effect is improved, and the additive is suitable for improving oral cavity dysfunctions such as the xerostomia.

Description

Technical Field

[0001] This invention relates to the field of food functional ingredients and biomaterials technology, and in particular to an oral lubricant composition, additive, preparation method and application thereof. Background Technology

[0002] Saliva, the most important biological lubricant in the oral cavity, is a complex mixture secreted by the salivary glands. Mucin, as the main functional protein, binds with water molecules to form a viscoelastic gel layer, creating a continuous lubricating and protective film on the surfaces of teeth and mucous membranes. However, reduced saliva secretion (xerostomia) caused by pathological factors such as radiotherapy, drug side effects, or Sjögren's syndrome can lead to loss of oral lubrication, resulting in problems such as difficulty swallowing, mucosal damage, and a high incidence of dental caries.

[0003] In existing technologies, clinically used artificial saliva is mainly divided into two categories: one is ionic artificial saliva, which is mainly composed of inorganic compounds. Although this type of product can temporarily relieve dry mouth, its poor membrane adsorption results in a short residence time, and it lacks the viscoelasticity of natural saliva, leading to insufficient lubrication. The other type is mucin-based artificial saliva, which is mainly composed of animal-derived mucins (such as placental globulin used in CN114504646B). Although its lubrication performance is closer to that of natural saliva, it often has a noticeable odor (such as a fishy smell) and high cost. In addition, high concentrations of mucin can easily cause a foreign body sensation in the mouth, while low concentrations are difficult to form a long-lasting lubricating film, making it difficult to fully simulate the dynamic balance of the glycoprotein-hydration network in natural saliva.

[0004] Patent application CN118766892A discloses a lubricating and antibacterial artificial saliva aid, the core of which lies in a composite system of anhydrous calcium chloride (5-12 wt%) and epigallocatechin gallate (EGCG, 0.1-0.5 wt%). This scheme utilizes calcium ions to significantly promote the adsorption and aggregation of salivary proteins on the oral cavity surface, thereby forming a thicker, more viscoelastic lubricating film. Simultaneously, EGCG exerts a good antibacterial effect, allowing the mixed saliva film to improve lubrication while inhibiting the growth of harmful bacteria in the oral cavity. Although this aid can enhance the lubricating properties of natural saliva to some extent, its application depends on the use of natural saliva, and the high concentration of calcium salts may cause a dry, astringent feeling in the mouth, limiting its independent use.

[0005] Chinese patent application CN106729649B discloses an oral care aid for treating dry mouth, whose main components include olive extract, lactoferrin microspheres, and zinc oxide microspheres. This aid achieves some effect in improving dry mouth symptoms by stimulating saliva secretion, reducing halitosis, and exerting antibacterial effects. However, due to significant differences in its composition compared to natural saliva, it has a poor taste and is difficult to form a stable, long-lasting lubricating film in the oral cavity. Therefore, it still has significant shortcomings in mimicking the multiple physiological functions of natural saliva.

[0006] In summary, while ionic artificial saliva can temporarily relieve dry mouth, it suffers from poor adsorption and insufficient lubrication. Animal-derived mucin-based products, although possessing better lubricating properties, exhibit noticeable odor, are costly, and struggle to regulate the dynamic balance of the glycoprotein-hydration network. Patents CN118766892A and CN106729649B attempt to address these issues through a calcium ion / EGCG complex system and a multi-component care aid, respectively, but each has its limitations. Summary of the Invention

[0007] The purpose of this invention is to provide an oral lubricating composition, an additive, a preparation method thereof, and its application. The preparation process is simple and controllable. This lubricating additive has good biocompatibility, significantly improves the lubrication effect in the oral cavity, relieves oral dryness, improves the oral digestion effect of food, and is suitable for improving oral dysfunctions such as xerostomia.

[0008] To achieve the above objectives, the present invention provides an oral lubricant composition comprising the following components: Lubricant enhancer Interface stabilizers; The lubricant enhancer is one or more of sugar alcohol compounds; The interface stabilizer is one or more of mammalian mucins.

[0009] Preferably, the sugar alcohol compound is one or more of glycerol, sorbitol, xylitol, and maltitol.

[0010] Preferably, the mammalian-derived mucin is one or both of gastric-derived mucin or salivary-derived mucin.

[0011] Preferably, the salivary mucin is bovine submandibular mucin, and the gastric mucin is porcine gastric mucin.

[0012] The present invention also provides an oral lubricant, comprising a liquid and the oral lubricant composition thereof.

[0013] This invention also provides a method for preparing an oral lubricant, comprising the following steps: S1. The interface stabilizer is purified and freeze-dried to obtain mucin powder; S2. Dissolve the mucin powder obtained in S1 in water to obtain a mucin solution with a concentration of 10-100 mg / mL; S3. Add the lubricating enhancer to the mucin solution obtained in S2, and react for 10-30 minutes under stirring at 15-45℃ to obtain an oral lubricating agent with a volume ratio of lubricating enhancer to mucin solution of 0.5-10:1.

[0014] Preferably, in S1, the purification is performed using one of the following: ultrafiltration membrane dialysis, gel permeation chromatography, ion exchange chromatography, ethanol precipitation, or ultrafiltration centrifugation.

[0015] Preferably, the ultrafiltration membrane dialysis specifically comprises: The interface stabilizer was dissolved in water to prepare a mucin solution with a concentration of 10-100 mg / mL. The solution was then subjected to dialysis using an ultrafiltration membrane with a molecular weight cutoff of 100 kDa for 7 days, with the dialysis water changed twice daily.

[0016] The present invention also provides an oral lubricant for the application of oral lubrication in patients with dry mouth, the elderly, and postoperative patients, and for relieving dry mouth caused by radiotherapy, the elderly, and denture wearing.

[0017] Preferably, the oral lubricant is one of an oral spray, a mouthwash, or an oral gel.

[0018] Therefore, the present invention employs the above-mentioned oral lubricating composition, additives, preparation method, and application, and the beneficial effects are as follows: The preparation process of this invention is simple and controllable, uses food-grade safe substances, is green and low-cost, and can be applied in the fields of oral care and medical care. It is suitable for industrial-scale production and clinical application.

[0019] The lubricant of this invention can be formulated into a liquid form for direct application or rinsing, or into an oral spray form for easy carrying and immediate use, meeting the oral lubrication needs of patients with dry mouth, the elderly, and postoperative patients. In particular, this lubricant does not rely on the function of salivary gland secretion and can be used to relieve symptoms of dry mouth caused by radiotherapy, old age, denture wearing, etc.

[0020] The lubricant of this invention has good biocompatibility. Through hydrogen bonding between sugar alcohol and mucin, it forms a continuous and stable hydrated lubricating film on the oral mucosa. This film has good viscoelasticity, moisture retention capacity, and interfacial adhesion. Its lubricating performance on simulated oral surfaces is similar to that of human saliva, with a friction coefficient of less than 0.1. It can significantly reduce the friction coefficient of oral contact interfaces. The adsorption frequency shift value on the PDMS interface, as tested by a dissipative quartz crystal microbalance (QCM-D), is not less than -70Hz, which can significantly improve the lubrication effect in the oral cavity. By introducing polyhydroxy sugar alcohol, it significantly enhances the film-forming ability of mucin, improves the odor and foreign body sensation in the mouth caused by high protein content, and effectively makes up for the shortcomings of existing artificial saliva, such as poor lubrication, strong odor, and unstable film. It achieves long-lasting lubrication and excellent taste, relieves oral dryness, improves the oral digestion effect of food, and is suitable for improving oral dysfunction such as xerostomia.

[0021] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Attached Figure Description

[0022] Figure 1 This is a friction curve diagram of an oral lubricating composition, additives, preparation method and application example 1 and comparative example 1 of the present invention; Figure 2 This is a friction curve diagram of an oral lubricating composition, additives, preparation method and application of the present invention, and Example 3 and Comparative Example 2. Figure 3 These are friction curves of Examples 1, 5, 6, 7 and Comparative Examples 1, 3, 4, 5 of the present invention, which describe an oral lubricating composition, additives, preparation method, and application. Figure 4 These are friction curves of an oral lubricating composition, additives, preparation method, and application examples 1, 2, 5, 6, 7, 8, 9, and 10 of the present invention. Figure 5 This is the QCM-D test result of an oral lubricating composition, additive, preparation method and application example of the present invention, wherein A is the membrane frequency (Δf) and dissipation change (ΔD) curve of glycerol on saliva, B is the effect of glycerol on the membrane frequency and dissipation change of adsorbed mucin, C is the adsorption of glycerol on PDMS surface, D is the adsorption of mucin on PDMS surface, and E is the adsorption of saliva on PDMS surface. Figure 6 This is a molecular docking simulation diagram of glycerol and mucin molecules in Example 1 of the present invention, which describes an oral lubricating composition, additives, preparation method, and application of the present invention. Figure 7 This is a molecular docking simulation diagram of xylitol and mucin molecules in Example 5 of the present invention, which describes an oral lubricating composition, additives, preparation method, and application of the present invention. Figure 8 This is a molecular docking simulation diagram of sorbitol and mucin molecules in Example 6 of the present invention, which describes an oral lubricating composition, additives, preparation method, and application of the present invention. Figure 9 This is a molecular docking simulation diagram of maltitol and mucin molecules in Example 7 of the present invention, which describes an oral lubricating composition, additives, preparation method, and application of the present invention. Detailed Implementation

[0023] The technical solution of the present invention will be further described below with reference to the accompanying drawings and embodiments.

[0024] Unless otherwise defined, the technical or scientific terms used in this invention shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention pertains.

[0025] Human saliva used in the experiment was provided by healthy volunteers. Volunteers were required to complete a health screening and avoid consuming high-sugar, high-acid, or caffeinated foods for two hours prior to collection. Subjects rinsed their mouths with distilled water for 1 minute, waited 5 minutes, and then spat their naturally secreted saliva into a sterile sampling tube. Saliva samples were placed in an ice bath and transferred to the laboratory within 1 hour. They were then centrifuged at 4°C and 4000g for 3 minutes to remove impurities. The supernatant was used for the experiment, and the remaining samples were stored at -80°C for later use. Informed consent was obtained from all volunteers for all saliva collections, and the process was approved by the ethics committee.

[0026] Example 1 An oral lubricant based on sugar alcohol-mucin interaction comprises the following components: glycerol (analytical grade, purity >99%), Type IS bovine mandibular mucin (BSM), and Milli-Q water.

[0027] Its preparation method includes the following steps: S1. Dissolve BSM in Milli-Q water to prepare a BSM solution with a concentration of 30 mg / mL. Dialyze the solution using an ultrafiltration membrane with a molecular weight cutoff of 100 kDa for 7 days, changing the dialysis water twice a day. Freeze dry to obtain BSM powder. S2. Mix BSM powder and Milli-Q water to obtain a 30 mg / mL BSM solution; S3. Mix glycerin and BSM solution at a volume ratio of 4:1 to obtain an oral lubricant.

[0028] Example 2 An oral lubricant based on sugar alcohol-mucin interaction, comprising the following components: Glycerol (analytical grade, purity >99%), Type II porcine gastric mucin (PGM), and Milli-Q water.

[0029] Its preparation method includes the following steps: S1. Dissolve PGM in Milli-Q water to prepare a PGM solution with a concentration of 30 mg / mL. Dialyze the solution using an ultrafiltration membrane with a molecular weight cutoff of 100 kDa for 7 days, changing the dialysis water twice a day. Freeze-dry the solution to obtain PGM powder. S2. Mix PGM powder with Milli-Q water to obtain a PGM solution of 30 mg / mL; S3. Mix glycerin and PGM solution at a volume ratio of 4:1 to obtain an oral lubricant.

[0030] Example 3 An oral lubricant based on sugar alcohol-mucin interaction comprises the following components: glycerol (analytical grade, purity >99%), Type IS bovine mandibular mucin (BSM), and Milli-Q water.

[0031] Its preparation method includes the following steps: S1. Dissolve BSM in Milli-Q water to prepare a BSM solution with a concentration of 30 mg / mL. Dialyze the solution using an ultrafiltration membrane with a molecular weight cutoff of 100 kDa for 7 days, changing the dialysis water twice a day. Freeze dry to obtain BSM powder. S2. Mix BSM powder and Milli-Q water to obtain a 30 mg / mL BSM solution; S3. Mix glycerin and BSM solution at a volume ratio of 1:1 to obtain an oral lubricant.

[0032] Example 4 An oral lubricant based on sugar alcohol-mucin interaction, comprising the following components: Glycerol (analytical grade, purity >99%), Type II porcine gastric mucin (PGM), and Milli-Q water.

[0033] Its preparation method includes the following steps: S1. Dissolve PGM in Milli-Q water to prepare a PGM solution with a concentration of 30 mg / mL. Dialyze the solution using an ultrafiltration membrane with a molecular weight cutoff of 100 kDa for 7 days, changing the dialysis water twice a day. Freeze-dry the solution to obtain PGM powder. S2. Mix PGM powder with Milli-Q water to obtain a PGM solution of 30 mg / mL; S3. Mix glycerin and PGM solution at a volume ratio of 1:1 to obtain an oral lubricant.

[0034] Example 5 An oral lubricant based on sugar alcohol-mucin interaction, differing from Example 1 in that glycerol is replaced with xylitol.

[0035] Example 6 An oral lubricant based on sugar alcohol-mucin interaction, differing from Example 1 in that glycerol is replaced with sorbitol.

[0036] Example 7 An oral lubricant based on sugar alcohol-mucin interaction, differing from Example 1 in that glycerol is replaced with maltitol.

[0037] Example 8 An oral lubricant based on sugar alcohol-mucin interaction, which differs from Example 2 in that glycerol is replaced with xylitol.

[0038] Example 9 An oral lubricant based on sugar alcohol-mucin interaction, which differs from Example 2 in that glycerol is replaced with sorbitol.

[0039] Example 10 An oral lubricant based on sugar alcohol-mucin interaction, which differs from Example 2 in that glycerol is replaced with maltitol.

[0040] Comparative Example 1 The difference from Example 1 is that the BSM solution was replaced with human saliva.

[0041] Comparative Example 2 The difference from Example 3 is that the BSM solution was replaced with human saliva.

[0042] Comparative Example 3 The difference from Example 5 is that the BSM solution was replaced with human saliva.

[0043] Comparative Example 4 The difference from Example 6 is that the BSM solution was replaced with human saliva.

[0044] Comparative Example 5 The difference from Example 7 is that the BSM solution is replaced with human saliva.

[0045] Test 1. Tribological tests were performed on the oral lubricants based on sugar alcohol-mucin interactions prepared in Examples 1-10 and Comparative Examples 1-5. The test methods are as follows: The tribological properties of the samples were measured using an MTM miniature traction machine equipped with a PDMS ball-disc friction contact surface. The PDMS ball had a diameter of 19 mm, the PDMS disk had a diameter of 46 mm and a thickness of 4 mm, both had a Young's modulus of 2.4 MPa, and an average surface roughness of Ra ~ 50 nm. The sample was loaded into a test container equipped with a PDMS disk, the PDMS ball was lowered onto the disk, and then the test container was covered with a lid. The PDMS ball and disk rotated at different speeds, generating relative motion between the surfaces of the ball and disk, thus producing a sliding-roll ratio, simultaneously imparting rolling and sliding motion. To simulate tongue / palate contact, the temperature was set to 37 °C, the contact normal force to 2.0 N, the SRR to 50%, and the entrainment speed (…). U The friction coefficient is 1~1000 mm / s. μ The change in ) was plotted as entrainment speed ( U The function is . The formula is:

[0046] in, μ The coefficient of friction, ( ) represents a functional relationship. η Viscosity (unit: Pa·s) U The conveyor speed is measured in mm / s. Normal load (in N).

[0047] The effects of different factors on the frictional behavior curves of the mixture were investigated by varying the mixing ratio (4:1 and 1:1), the type of sugar alcohol (glycerol, sorbitol, xylitol, maltitol), and the type of mucin (BSM, PGM). The test results are as follows: Figures 1-4 As shown.

[0048] Depend on Figure 1 It is known that sugar alcohols (glycerol) significantly reduce the coefficient of friction of the system when mixed with mucin or human saliva, indicating that the introduction of sugar alcohols improves the lubrication performance of the system. More importantly, although BSM and human saliva have significantly different frictional properties, their frictional behavior tends to be consistent after mixing with glycerol. This suggests that glycerol specifically interacts with biomolecules in mucin or saliva to construct a structure similar to the natural saliva lubricating film. This indicates that the sugar alcohol-mucin system proposed in this invention can mimic the lubrication function of saliva and holds promise as a saliva alternative to address the lack of lubrication caused by dry mouth or other oral dryness problems.

[0049] Depend on Figure 2It can be seen that after changing the mixing ratio of sugar alcohol and mucin (from 4:1 to 1:1), the friction behavior of the resulting system still exhibits the typical characteristics of the lubrication zone dominance. This further illustrates that this type of sugar alcohol-mucin lubrication system can still stably form a lubricating film under ratio control, has formulation flexibility, and can be adjusted according to application needs, thereby improving its applicability in oral care products.

[0050] Depend on Figure 3 It is known that different sugar alcohols (glycerol, xylitol, sorbitol, and maltitol) can effectively reduce the coefficient of friction after mixing with mucin or saliva, and the curve shapes remain consistent. This result indicates that different sugar alcohol molecules interact with mucin through similar mechanisms to form a stable lubricating structure. This universality gives the present invention broad scalability and application prospects, and helps to optimize formulations for individual differences to achieve personalized lubrication solutions.

[0051] Depend on Figure 4 It is known that different types of mucins (BSM and PGM) can significantly improve lubrication performance after interacting with various sugar alcohols, indicating that the lubricating agent proposed in this invention has adaptability to mucin sources, which is of great significance for solving the technical bottleneck caused by species differences or production raw material limitations.

[0052] 2. The dynamic adsorption behavior of BSM / human saliva and glycerol on the surface of the PDMS sensor was tested in Examples 1, 3, Comparative Example 1, and Comparative Example 3. The test methods are as follows: The PDMS sensor was sequentially immersed in toluene, isopropanol, and MilliQ water for 30 seconds, 30 seconds, and 5 minutes, respectively, then dried with nitrogen and placed in a fume hood for 1 hour to allow the remaining solvent to evaporate completely. The cleaned PDMS sensor was placed on the flow module of the QCM, and all solutions were supplied to the QCM-D chamber containing the PDMS sensor chip at a flow rate of 100 μL / min at 25°C using a peristaltic pump. HEPES buffer solution was injected first until a stable baseline was observed. Subsequently, mucin solution or diluted human saliva was injected for at least 1 hour to equilibrate the system. After equilibration, the system was rinsed again with HEPES buffer, and after stabilization, sugar alcohol solution was injected. The data were fitted using the Voigt model (i.e., the Smartfit model) of viscoelastic solids using Dfind software to obtain the mass of the hydrated protein layer. This model treats the adsorbed layer as a viscoelastic body with shear modulus μ and shear viscosity η. The model, based on frequency and energy dissipation data and incorporating the physical parameters of the liquid and quartz crystal, was fitted using the following complex expression:

[0053] Where Δf is the frequency change, ΔD is the energy dissipation change, i is the imaginary unit, f0 is the fundamental frequency of the sensor (in Hz), n is the harmonic order, and ρ q The density of quartz crystals (unit: kg / m³) 3 ), d q η is the thickness of the quartz crystal (in meters), μ1 is the shear modulus of the adsorption layer (in Pa), η1 is the shear viscosity of the adsorption layer (in Pa·s), and ω is the angular frequency (in rad / s). The test results are as follows: Figure 5 As shown.

[0054] Depend on Figure 5 As can be seen from the frequency and dissipation curves, the addition of sugar alcohol not only enhances the thickness of the adsorption layer but also drives the formation of a multilayer structure through hydrogen bonding, thereby enhancing the system's hydration and adhesion, and achieving a more stable and viscoelastic lubricating film structure. This structural feature is precisely the key functional property required by natural saliva lubricating films, further verifying that the present invention can effectively simulate saliva films and achieve a technical replacement for lubrication deficiencies.

[0055] 3. The binding energy and interaction modes between different sugar alcohol molecules and mucin molecules in Examples 1 and 5-7 were evaluated using the following methods: The binding energies and interaction modes between different sugar alcohol molecules and mucin molecules were evaluated using the AutodockVina 1.2.2 protein-small molecule docking software (https: / / autodock.scripps.edu / ). The 3D structures of glycerol, sorbitol, xylitol, and maltitol molecules were obtained from PubChem (https: / / pubchem.ncbi.nlm.nih.gov / ) (PubChem-CID:753;5780;6912;493591) and their energy minimization was performed using Chem3D 20.0. The 3D structure of mucin (PDB ID:5AJN) was obtained from the RCSB Protein Data Bank database (https: / / www.rcsb.org / ) and pretreated using the ProteinPreparation module of Maestro 12.7, including hydrogenation, dehydration, and energy minimization. The processed target protein and ligand were imported into the computational platform, a docking range was selected, and molecular docking simulations were performed. The final results were visualized using AutodockVina 1.2.2, and the conformation with the lowest binding energy was used to describe the binding mode. The evaluation results are as follows: Figures 6-9 As shown.

[0056] Depend on Figures 6-9It was found that all four sugar alcohols could interact with mucin through hydrogen bonding, with low binding energy and concentrated binding sites, indicating that sugar alcohol molecules play a structural induction role in the construction of the mucin adsorption film. Combined with the tribological test and QCM-D results, it can be inferred that the hydrogen bond network between sugar alcohols and mucins is the core driving mechanism for the formation of a stable lubricating film and the molecular basis for solving lubrication deficiencies.

[0057] Therefore, the present invention employs the above-mentioned oral lubricating composition, additive, preparation method and application. The preparation process is simple and controllable. This lubricating additive has good biocompatibility, significantly improves the lubrication effect in the oral cavity, relieves oral dryness, improves the oral digestion effect of food, and is suitable for improving oral dysfunction such as xerostomia.

[0058] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solutions of the present invention, and these modifications or equivalent substitutions cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. An oral lubricant composition, characterized in that, Includes the following components: Lubricant enhancer Interface stabilizers; The lubricant enhancer is one or more of sugar alcohol compounds; The interface stabilizer is one or more of mammalian mucins.

2. The oral lubricant composition according to claim 1, characterized in that: The sugar alcohol compound is one or more of glycerol, sorbitol, xylitol, and maltitol.

3. The oral lubricant composition according to claim 1, characterized in that: The mammalian-derived mucin is one or both of gastric-derived mucin or salivary-derived mucin.

4. The oral lubricant composition according to claim 3, characterized in that: The salivary mucin was bovine submandibular mucin, and the gastric mucin was porcine gastric mucin.

5. An oral lubricant, characterized in that, Includes liquids and the oral lubricating composition according to any one of claims 1-4.

6. A method for preparing an oral lubricant, characterized in that, Includes the following steps: S1. The interface stabilizer is purified and freeze-dried to obtain mucin powder; S2. Dissolve the mucin powder obtained in S1 in water to obtain a mucin solution with a concentration of 10-100 mg / mL; S3. Add the lubricating enhancer to the mucin solution obtained in S2, and react for 10-30 minutes under stirring at 15-45℃ to obtain an oral lubricating agent with a volume ratio of lubricating enhancer to mucin solution of 0.5-10:

1.

7. The method for preparing an oral lubricant according to claim 6, characterized in that, In S1, the purification is performed using one of the following methods: ultrafiltration membrane dialysis, gel permeation chromatography, ion exchange chromatography, ethanol precipitation, or ultrafiltration centrifugation.

8. The method for preparing an oral lubricant according to claim 7, characterized in that, The ultrafiltration membrane dialysis specifically refers to: The interface stabilizer was dissolved in water to prepare a mucin solution with a concentration of 10-100 mg / mL. The solution was then subjected to dialysis using an ultrafiltration membrane with a molecular weight cutoff of 100 kDa for 7 days, with the dialysis water changed twice daily.

9. The oral lubricant prepared by the method of any one of claims 6-8 is used for oral lubrication and relief of dry mouth caused by radiotherapy, the elderly, and dentures in patients with dry mouth, the elderly, and postoperative patients.

10. The application according to claim 9, characterized in that: The oral lubricant is one of the following: oral spray, mouthwash, or oral gel.

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