Microfluid device and microfluid detection device

Abstract

The invention discloses a microfluid device and a microfluid detection device. The microfluid device comprises a body (100) and a fluid channel (201) arranged in the body (100), the body (100) is further provided with a fluid input port (103) communicated with the fluid channel (201), and the arrangement position of the fluid input port (103) does not include the sensing area (301). The microfluid device and the microfluid detection device are convenient to operate, and can effectively solve the problem that bubbles are introduced due to the fact that a liquid injection hole is directly formed above the sensing area (301).

Description

[0001] This application is a divisional application of the original application with the original application number 202180101430.0, the original application date of December 10, 2021, and the original invention title of "Microfluidic device and microfluidic detection device". TECHNICAL FIELD

[0002] The present application relates to the technical field of microfluidics, in particular to a microfluidic device and a microfluidic detection device. BACKGROUND

[0003] Microfluidic technology is a technology for controlling, operating and detecting complex fluids at a microscale. It is a new cross-disciplinary subject developed on the basis of microelectronics, micromechanics, bioengineering and nanotechnology. In biological, chemical, material and other scientific experiments, fluid operations are often required, such as sample DNA preparation, liquid chromatography, PCR reaction, electrophoresis detection, etc. These operations are carried out in a liquid phase environment.

[0004] If the sample preparation, biochemical reaction, result detection and other steps are integrated on a biochip, the amount of fluid used in the experiment will usually be reduced from milliliters to microliters. At this time, a special microfluidic device is needed to meet the operation requirements. Microfluidic devices have the advantages of small size, small amount of sample / reagent used, fast reaction speed, etc. and are widely used in biological technology research.

[0005] However, the microfluidic device in the prior art usually has a liquid injection hole (as shown in FIG. 33) above the sensing area, which is used to add reagents or samples. This is easy to introduce air bubbles into the sensing area and is not easy to operate. Figure 1a And Figure 1b In view of the above, the present application is proposed.

[0006] In view of the above, the present application is proposed. SUMMARY

[0007] The present application provides a microfluidic device and a microfluidic detection device to solve the problem of air bubbles introduced by the microfluidic device in the prior art due to the liquid injection hole above the sensing area.

[0008] To solve the above problems and improve the integration of the biomolecular detection device and reduce the size of the detection device, the present application adopts the following scheme: A microfluidic device, comprising a body and a liquid channel arranged in the body; A sensing area is arranged on the body; A fluid input port in communication with the liquid channel is further arranged on the body, and the setting position of the fluid input port does not include the sensing area.

[0009] In the scheme, the microfluidic device comprises a body and a liquid channel arranged inside the body, the body is provided with a sensing area, and the liquid in the sensing area can be sensed as required; the body is also provided with a fluid input port in communication with the liquid channel, and the fluid input port is not arranged in the sensing area, that is, the fluid input port is not arranged directly above the sensing area; the to-be-detected liquid enters the liquid channel from the fluid input port and flows into the sensing area to be sensed, which is convenient to operate and can effectively solve the problem of air bubbles introduced due to the direct opening of the liquid injection hole above the sensing area.

[0010] In a further preferred scheme, the body is also provided with an on-off device for controlling the flow and interruption of the liquid in the liquid channel. By interrupting the liquid in the liquid channel with the on-off device, the liquid in the sensing area is continuously and stably sensed, and the sensing efficiency is improved.

[0011] In a further preferred scheme, the on-off device is provided with a flow channel capable of communicating with the liquid channel; the on-off device controls whether the flow channel communicates with the liquid channel by relative movement with the body. When the on-off device is adjusted so that the two ends of the flow channel in the on-off device communicate with the liquid channel, the liquid normally flows; when the two ends of the flow channel in the on-off device do not communicate with the liquid channel, the on-off device interrupts the liquid in the liquid channel at this time.

[0012] In a further preferred scheme, at least one hollow sealed chamber is arranged in the body, and a flow-through membrane is arranged at the top of the at least one sealed chamber, and the sealed chamber and the liquid channel share the flow-through membrane. The liquid in the sealed chamber and the liquid in the liquid channel are independent of each other, and ion transmission or other forms of signal transmission between the two liquids can be achieved through the flow-through membrane.

[0013] In a further preferred scheme, at least one liquid passage is arranged on the body, and the liquid passage communicates with the sealed chamber. The liquid in the sealed chamber can be injected through the liquid passage, and the number of liquid passages can be adjusted as required.

[0014] In a further preferred scheme, a sealing member or a conductive member is arranged at the bottom of the sealed chamber, and the conductive member is used to transmit an electrical signal, and the sealing member is mainly used to close the bottom of the sealed chamber.

[0015] In a further preferred scheme, the sealed chamber comprises at least a cavity A and a cavity B in communication with each other, and at least one liquid inlet or outlet is arranged on the cavity A and the cavity B respectively, and the two liquid inlets and outlets cooperate with each other without interfering with each other.

[0016] In a further preferred scheme, the conductive member is arranged at the bottom of the cavity A and is used to transmit an electrical signal to the outside.

[0017] In a further preferred embodiment, the flow-through membrane is arranged on the top of the cavity B, and the bottom of the cavity B is provided with the sealing member. The flow-through membrane is used to realize ion transfer or other forms of signal transfer between the liquid in the cavity B and the liquid in the liquid channel; and the sealing member is used to seal the bottom of the cavity B to prevent the liquid from leaking out.

[0018] In a further preferred embodiment, the cavity A is in the shape of a shuttle and is arranged on one side of the liquid channel, the cavity B is arranged below the liquid channel, and the tip of the cavity A opposite to the liquid channel is in communication with the cavity B. The cavity B is arranged below the liquid channel, and the flow-through membrane arranged on the top of the cavity B can realize signal transfer with the liquid channel. The tip of the cavity A is in communication with the cavity B, thereby forming a sealed area as a whole and expanding the liquid storage capacity of the sealed area to a certain extent.

[0019] In a further preferred embodiment, a fluid storage device is arranged in communication with the fluid input port, and the top of the fluid storage device is open and the bottom of the fluid storage device is in communication with the fluid input port. Since the fluid input port of the microfluidic device is usually small in size, it is inconvenient to add liquid directly through the fluid input port. In this embodiment, the fluid storage device can be used as a liquid adding device, and the top of the fluid storage device is large in size, thereby facilitating liquid addition.

[0020] In a further preferred embodiment, the fluid storage device is detachably arranged on the body, and the interior of the fluid storage device is in the shape of a funnel. The fluid storage device is detachably arranged on the body, thereby facilitating installation, disassembly and replacement. The interior of the fluid storage device is in the shape of a funnel, which is wide at the top and narrow at the bottom, thereby facilitating liquid filling.

[0021] In a further preferred embodiment, the area of the body opposite to the position of the sensing area is transparent, thereby facilitating observation of the state of the liquid in the sensing area.

[0022] In a further preferred embodiment, the body is further provided with a collection area, the collection area is in communication with the tail end of the liquid channel, and a liquid discharge port is arranged on the collection area. After the liquid in the liquid channel is sensed, the liquid can enter the collection area for collection. When the volume of the liquid in the collection area is greater than the volume of the collection area, the liquid can be discharged from the collection area through the liquid discharge port, thereby realizing the function of cyclic collection.

[0023] In a further preferred embodiment, the collection area is in the shape of a spiral or a snake. The spiral or snake-shaped structure can effectively increase the volume of the collection area, thereby facilitating collection of more liquid.

[0024] In a further preferred embodiment, the body comprises an upper plate and a lower plate connected to each other, and the liquid channel is a cavity formed between the upper plate and the lower plate. The bottom of the liquid channel is the upper surface of part of the lower plate, and the top of the liquid channel is the lower surface of part of the upper plate. The liquid channel is formed by the special structure of the upper plate and the lower plate, resources are fully utilized, and the structure of the upper plate and the lower plate also plays an important role in the whole microfluidic device.

[0025] In a further preferred scheme, the area opposite to the position of the sensing area of the upper plate is recessed towards the lower plate, facilitating observation of the state of the liquid in the sensing area; and the area opposite to the position of the sensing area of the lower plate is hollowed out, and the hollowed-out area is used to externally connect a sensing device.

[0026] In a further preferred scheme, the collection area is further provided with a vent hole. A negative pressure device can be externally connected at the vent hole, and the liquid in the fluid storage device is sucked into the liquid channel and the sensing area by applying negative pressure.

[0027] In a further preferred scheme, a control valve is arranged between the fluid input port and the fluid storage device, and the control valve is used to control whether the fluid input port is in communication with the fluid storage device. When the control valve is opened, the fluid input port is in communication with the fluid storage device, and at this time, the fluid storage device can be directly used to add liquid.

[0028] The present scheme also provides a microfluidic detection device comprising the above microfluidic device, and the microfluidic detection device further comprises: a sensing device connected to the body and corresponding to the sensing area; The sensing device and the liquid channel have an overlapping part, and the liquid in the liquid channel can flow through the overlapping part.

[0029] In the present scheme, the sensing device and the hollowed-out position of the sensing area correspond, and the sensing device and the liquid channel have an overlapping part, and the sensing device can be used to sense the liquid in the sensing area.

[0030] In a further preferred scheme, a sealing element is arranged between the sensing device and the body. The arrangement of the sealing element can effectively prevent liquid leakage at the connection between the sensing device and the body.

[0031] In a further preferred scheme, the sensing device is detachably connected to the lower plate, and the sealing element is detachably connected to the sensing device. The detachable connection facilitates installation and disassembly of the sensing device.

[0032] In a further preferred scheme, the sensing device comprises a carrier plate; and A sensing chip is arranged on the carrier plate, and the sensing chip corresponds to the liquid channel in the sensing area. In use, the liquid in the liquid channel flows through the upper surface of the sensing chip and is sensed by the sensing chip. The carrier plate mainly supports and fixes the sensing chip, and the carrier plate and the sensing chip are combined as a whole sensing device.

[0033] In a further preferred solution, the sealing element is a sealing sheet, and a through hole is arranged in the middle of the sealing sheet, and the liquid channel and the sensing chip are communicated through the through hole. The middle of the sealing sheet is provided with a through hole, and the liquid can flow into the through hole and reach the surface of the sensing chip to be sensed. The sensing area can be effectively sealed by the sealing sheet.

[0034] In a further preferred solution, the cross-sectional area of the through hole is smaller than the upper surface area of the sensing chip, and the edge of the through hole is located above the sensing chip. The through hole is completely located above the sensing chip, that is, the liquid in the through hole can be fully sensed by the upper surface of the sensing chip.

[0035] Compared with the prior art, the present application has the following beneficial effects: The present application provides a microfluidic device, which comprises a body and a liquid channel arranged in the interior of the body. The body is provided with a sensing area, and the fluid input port is not arranged directly above the sensing area. The liquid in the sensing area can be sensed as required. The liquid to be tested enters the liquid channel from the fluid input port and flows into the sensing area to be sensed. The operation is convenient, and the problem of introducing air bubbles caused by directly opening the liquid injection hole above the sensing area can be effectively solved.

[0036] The present application also provides a microfluidic detection device comprising the above-mentioned microfluidic device. The liquid to be detected can be directly sensed by the microfluidic detection device. The operation is convenient, and the practicability is strong. BRIEF DESCRIPTION OF DRAWINGS

[0037] In order to more clearly illustrate the specific embodiments of the present application or the technical solutions in the prior art, the drawings needed in the following specific embodiment or prior art description will be briefly introduced. Obviously, the drawings in the following description are some embodiments of the present application. Those skilled in the art can obtain other drawings without creative labor on the basis of these drawings.

[0038] Figure 1a And Figure 1b The structure schematic diagram of the prior art microfluidic device described in the background art; Figure 2 The specific structure schematic diagram of the microfluidic device described in the present application; Figure 3 The Figure 2 The A-A sectional view in the above-mentioned embodiment. Figure 4 A specific structural exploded view of the microfluidic detection device according to the present application; Figure 5 Another angle of the structural exploded view of the microfluidic detection device according to the present application; Figure 6 Another specific structural schematic view of the microfluidic device according to the present application; Figure 7 A specific structural schematic view of the microfluidic device according to the present application; Figure 6 A cross-sectional view along B-B; Figure 8 A structural schematic view of the flow channel and the liquid channel of the on-off device according to the present application in the interrupted state; Figure 9 A cross-sectional view along C-C; Figure 8 A structural schematic view of the on-off device according to the present application; Figure 10 Figure 9 A cross-sectional view along D-D; Figure 11 A structural schematic view of the flow channel and the liquid channel of the on-off device according to the present application in the connected state; Figure 12 A cross-sectional view along E-E; Figure 11 A structural schematic view of the on-off device according to the present application; Figure 13 Figure 11 A cross-sectional view along F-F; Figure 14 A structural schematic view of the collection area according to the present application; Figure 15 Another structural schematic view of the collection area according to the present application.

[0039] In the above drawings, the components represented by the respective reference numerals are listed as follows: Body - 100; upper plate - 101; lower plate - 102; fluid input port - 103; on-off device - 104; fluid storage device - 105; collection area - 106; Liquid channel - 201; Sensing area - 301; Sealed chamber - 400; cavity A - 4001; cavity B - 4002; liquid passage - 401; flow-through membrane - 402; sealing component - 403; conductive component - 404; Sensing device - 500; carrier plate - 501; sensing chip - 502; sealing element - 503; Liquid discharge port - 601; air vent - 602. DETAILED DESCRIPTION

[0040] ​​In order to make the above and other features and advantages of the present application more comprehensible, the present application will be further described below with reference to the drawings. It is to be understood that the specific embodiments presented herein are by way of example only and are not limiting as to the scope of the present application.

[0041] In the description of the present application, it is to be understood that the orientations or positional relationships indicated by the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like are based on the orientations or positional relationships shown in the drawings, and are merely for the purpose of facilitating the description of the present application and simplifying the description, and are not intended to indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be construed as limiting the present application.

[0042] In addition, the terms "first", "second", "third", etc. are used only for the purpose of description and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features defined with "first", "second", etc. can explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "a plurality of" is at least two, such as two, three, etc., unless otherwise specifically limited.

[0043] In the present application, unless otherwise specifically defined and limited, the terms "mounting", "connecting", "connecting", "fixing" and the like should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or it can be integrated; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the internal communication of two elements or the interaction relationship between two elements, unless otherwise specifically defined. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0044] In the present application, unless otherwise specifically defined and limited, the first feature is "on" or "under" the second feature, which can be direct contact between the first and second features, or indirect contact between the first and second features through an intermediate medium. Moreover, the first feature "above", "above" and "above" the second feature can be directly above or obliquely above the first feature, or only indicate that the horizontal height of the first feature is higher than that of the second feature. The first feature "below", "below" and "below" the second feature can be directly below or obliquely below the first feature, or only indicate that the horizontal height of the first feature is less than that of the second feature.

[0045] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0046] Please refer to Figures 2-7 As shown, the present invention provides a microfluidic device, including a body 100 and a liquid channel 201 disposed within the body 100; a sensing area 301 is disposed on the body 100; a fluid input port 103 communicating with the liquid channel 201 is also disposed on the body 100, and the position of the fluid input port 103 does not include the sensing area 301.

[0047] In this scheme, the microfluidic device includes a body 100 and a liquid channel 201 disposed inside the body 100. The body 100 is provided with a sensing area 301. Liquid in the liquid channel 201 can flow through the sensing area 301, and the liquid in the sensing area 301 can be sensed as needed. The body 100 is also provided with a fluid input port 103 communicating with the liquid channel 201. The fluid input port 103 is not located above the sensing area 301, that is, the fluid input port 103 is not located directly above the sensing area 301, which can effectively solve the problem of introducing air bubbles due to directly opening a liquid injection hole above the sensing area.

[0048] Furthermore, the main body 100 is also provided with an on / off device 104, which is used to control the flow and interruption of liquid in the liquid channel 201. By using the on / off device 104 to interrupt the flow of liquid in the liquid channel 201, the flow of liquid in the liquid channel 201 is stopped, thereby ensuring that the liquid in the sensing area 301 is continuously and stably sensed, and improving the sensing efficiency.

[0049] It should be noted that the on / off device 104 is mainly used to control the flow and interruption of liquid in the liquid channel 201, and its specific structure and working principle are not limited here.

[0050] Furthermore, the switching device 104 is provided with a flow channel that can communicate with the liquid channel 201. The switching device 104 controls whether the flow channel and the liquid channel 201 are connected by relative movement with the body 100. When the switching device 104 is adjusted so that both ends of the flow channel in the switching device 104 are connected with the liquid channel 201, the liquid flows normally; when both ends of the flow channel in the switching device 104 are not connected with the liquid channel 201, the switching device 104 will interrupt the liquid in the liquid channel 201. In this solution, the manner in which the switching device 104 and the body 100 move relative to each other is not specifically limited, such as translation or rotation.

[0051] In another preferred embodiment, the on / off device 104 is a rotary valve, which has a flow channel that can communicate with the liquid channel 201. Rotating the rotary valve controls whether the flow channel and the liquid channel 201 are connected. Figures 8-10 As shown, when the rotary valve is in the "OFF" state, the flow channel and liquid channel 201 are disconnected, allowing for continuous and stable sensing of the liquid within the sensing area 301; Figures 11-13 As shown, when the rotary valve is in the "ON" state, the flow channel and the liquid channel 201 are in a connected state. At this time, the liquid in the liquid channel 201 can flow smoothly through the flow channel and enter the collection area 106 to be collected.

[0052] Furthermore, such as Figure 6 and Figure 7 As shown, the main body 100 also includes at least one hollow sealed chamber 400, and a flow membrane 402 is disposed on the top of at least one sealed chamber 400. The sealed chamber 400 and the liquid channel 201 share the flow membrane 402, that is, the sealed chamber 400 and the liquid channel 201 are separated into two independent spaces by the flow membrane 402. The liquid in the sealed chamber 400 and the liquid in the liquid channel 201 are independent of each other, and ion transfer or other forms of signal transfer can be achieved between the liquid in the sealed chamber 400 and the liquid in the liquid channel 201 through the flow membrane 402. The specific structure and material of the flow membrane 402 are not limited here, and can be made according to the actual functional requirements. For example, in some cases, the flow membrane 402 can be a semi-permeable membrane.

[0053] It should be understood that the sealed chamber 400 in this solution only needs to be located between the on / off device 104 and the fluid input port 103, but it can be located upstream or downstream of the sensing area 301.

[0054] Furthermore, the main body 100 is also provided with at least one liquid inlet 401, which communicates with the sealed chamber 400. Liquid can be added to the sealed chamber 400 through the liquid inlet 401, and the number of liquid inlets 401 can be adjusted as needed. In this design, the liquid inlets 401 can be used both to add liquid to the sealed chamber 400 and as a drain channel for the liquid in the sealed chamber 400.

[0055] Furthermore, such as Figure 7 As shown, a sealing component 403 or a conductive component 404 is provided at the bottom of the sealed chamber 400. The conductive component 404 is used to transmit electrical signals. Its specific shape and position are not limited in this embodiment. The sealing component 403 is mainly used to seal the bottom of the sealed chamber 400 to prevent liquid leakage.

[0056] Furthermore, such as Figure 4 As shown, the sealed chamber 400 includes at least two interconnected cavities, A4001 and B4002. Each cavity, A4001 and B4002, is provided with at least one inlet port 401 for liquid inlet or outlet. When the inlet port 401 on cavity A4001 is used for liquid inlet, the inlet port 401 on cavity B4002 is used for liquid outlet; similarly, when the inlet port 401 on cavity B4002 is used for liquid inlet, the inlet port 401 on cavity A4001 is used for liquid outlet. The two inlets 401 do not interfere with each other. The position and shape of cavities A4001 and B4002 can be arranged according to the detection requirements.

[0057] Furthermore, a conductive component 404 is disposed at the bottom of cavity A4001 for transmitting electrical signals to the outside. In another embodiment, the conductive component 404 may be a metal sheet or an electrode.

[0058] Furthermore, a flow membrane 402 is disposed at the top of cavity B4002, and a sealing member 403 is disposed at the bottom of cavity B4002. The flow membrane 402 is used to realize ion transfer or other forms of signal transfer between the liquid in cavity B4002 and the liquid channel 201; the sealing member 403 is used to seal the bottom of cavity B4002 to prevent liquid leakage. In another embodiment, the sealing member 403 may be a sheet-like object.

[0059] Since cavities A4001 and B4002 are interconnected and close together, and the conductive component 404 is located at the bottom of cavity A4001 and the flow membrane 402 is located at the top of cavity B4002, the conductive component 404 and the flow membrane 402 can be wetted by the same liquid.

[0060] Furthermore, cavity A4001 has a spindle-shaped structure and is located on one side of liquid channel 201. Cavity B4002 is located below liquid channel 201, and the tips of cavity A4001 opposite to liquid channel 201 are connected to cavity B4002. Cavity B4002 is located below liquid channel 201, and cavity B4002 can transmit signals with liquid channel 201 through a flow membrane 402 provided on its top. That is, the liquid in cavity B4002 and the liquid in liquid channel 201 can transmit ions or other forms of signals through the flow membrane 402. Cavity A4001 and cavity B4002 are connected, forming a sealed area.

[0061] It should be understood that the structures of cavities A4001 and B4002 can be adjusted as needed. The spindle-shaped structure of cavity A4001 in this design is only one embodiment; in other embodiments, cavity A4001 can also be elliptical or other shapes. Cavities A4001 and B4002 are detachably disposed within the body 100, and their installation can be selected as needed, or only one can be installed.

[0062] Furthermore, a fluid storage device 105 is connected to the fluid input port 103. The top of the fluid storage device 105 is open, and its bottom is connected to the fluid input port 103. Since the fluid input port 103 of the microfluidic device is usually small in size, it is not convenient to add liquid directly through the fluid input port 103. In this solution, the fluid storage device 105 can be used as a liquid adding device. Its top opening is large, which facilitates liquid adding and makes it less likely to spill.

[0063] Furthermore, the fluid storage device 105 is detachably mounted on the main body 100, and the interior of the fluid storage device 105 has a funnel-shaped structure. The detachable mounting of the fluid storage device 105 on the main body 100 facilitates installation, disassembly, and replacement. To ensure sufficient liquid entry into the liquid channel 201, the interior of the fluid storage device 105 is designed as a funnel-shaped structure, wider at the top and narrower at the bottom, thus facilitating liquid filling and minimizing liquid residue on the inner wall of the fluid storage device 105.

[0064] It should be noted that the fluid storage device 105 can be made into different shapes as needed, and the above-described funnel-shaped structure is only a preferred embodiment. The shape of the fluid inlet port 103 can also be adjusted according to actual needs, such as a round opening, a square opening, or a flared opening.

[0065] Furthermore, the area on the body 100 opposite to the position of the sensing area 301 is transparent, thereby facilitating the observation of the liquid state within the sensing area 301. Figure 2The middle circle only refers to the approximate range of the sensing area 301. The body 100 within this area can be made of a transparent material to allow for direct observation of the liquid state within the sensing area 301, thereby improving sensing efficiency. The shape of the sensing area 301 is not limited and can be adjusted according to actual needs.

[0066] Furthermore, the main body 100 also includes a collection area 106, which is connected to the tail end of the liquid channel 201. A drain port 601 is provided on the collection area 106. After the liquid in the liquid channel 201 has been completely sensed, the on / off device 104 is opened, allowing the liquid in the liquid channel 201 to enter the collection area 106 for collection. When the volume of liquid in the collection area 106 exceeds the capacity of the collection area 106, the liquid can be discharged from the collection area 106 through the drain port 601. For example, the liquid can be drawn out from the drain port 601 using a syringe, allowing the collection area 106 to continue collecting liquid, thus achieving a cyclic collection function.

[0067] Furthermore, such as Figure 14 and Figure 15 As shown, the collection area 106 has a spiral or serpentine structure. The spiral or serpentine structure can effectively increase the volume of the collection area 106, making it easier to collect more liquid.

[0068] It should be noted that the liquid collection area 106 can take various forms, such as a liquid storage tank or other storage media such as absorbent cotton. The collection area 106 is not limited to a cavity structure.

[0069] Furthermore, such as Figure 4 As shown, the body 100 includes an upper plate 101 and a lower plate 102 that are connected to each other, and the liquid channel 201 is a cavity formed between the upper plate 101 and the lower plate 102; The bottom of the liquid channel 201 is part of the upper surface of the lower plate 102, and the top of the liquid channel 201 is part of the lower surface of the upper plate 101. In actual use, the upper plate 101 and the lower plate 102 are permanently combined together by welding, gluing, bonding or other methods. The liquid channel 201 is formed by the special structure of the upper plate 101 and the lower plate 102, making full use of resources. Moreover, the structure of the upper plate 101 and the lower plate 102 also plays an important role in the entire microfluidic device.

[0070] It should be understood that the portion of the upper plate 101 within the sensing area 301 in this solution is made of a transparent material.

[0071] Furthermore, the area of ​​the upper plate 101 opposite to the sensing area 301 is recessed towards the lower plate 102, thereby reducing the thickness of the upper plate 101 in the sensing area 301, which facilitates the observation of the state of the liquid in the sensing area 301; the area of ​​the lower plate 102 opposite to the sensing area 301 is hollowed out, and the shape and size of the hollowed-out area can be adjusted as needed. The hollowed-out area is used for connecting an external sensing device 500.

[0072] Furthermore, a vent 602 is provided on the collection area 106. A negative pressure device can be connected to the vent 602 to draw the liquid in the fluid storage device 105 into the liquid channel 201 and into the sensing area 301 by applying negative pressure.

[0073] It should be added that the liquid can enter the liquid channel 201 from the fluid inlet port 103 by being drawn in by applying negative pressure downstream, by applying external pressure, or by expelling the air in the liquid channel 201 in advance to form a vacuum chamber, so that the fluid can be drawn in by itself.

[0074] Furthermore, a control valve is provided between the fluid inlet port 103 and the fluid storage device 105. The control valve is used to control whether the fluid inlet port 103 and the fluid storage device 105 are connected. When the control valve is open, the fluid inlet port 103 is connected to the fluid storage device 105, and liquid can be directly added through the fluid storage device 105. When the control valve is closed, the fluid inlet port 103 is isolated from the fluid storage device 105, and liquid cannot flow into the fluid inlet port 103.

[0075] like Figure 5 As shown, this solution also provides a microfluidic detection device including a microfluidic apparatus, the microfluidic detection device further including: A sensing device 500 connected to the main body 100 and corresponding to the sensing area 301; The sensing device 500 overlaps with the liquid channel 201, and liquid can flow through the liquid channel 201.

[0076] In this solution, the cutout positions of the sensing device 500 and the sensing area 301 correspond, and the sensing device 500 overlaps with the liquid channel 201. The sensing device 500 can be used to sense the liquid located in the sensing area 301.

[0077] In use, the sensing device 500 is sealed and installed at the hollow position of the sensing area 301, so that the liquid channel 201 is in a sealed state. The sensing device 500 is then part of the liquid channel 201. When liquid flows through the surface of the sensing device 500, it can be effectively sensed.

[0078] Furthermore, a sealing element 503 is provided between the sensing device 500 and the body 100. The sealing element 503 keeps the sensing area 301 sealed, which can effectively prevent liquid leakage at the connection between the sensing device 500 and the body 100, and also prevent the introduction of air bubbles, thereby improving sensing efficiency.

[0079] Furthermore, the sensing device 500 is detachably connected to the lower plate 102; the sealing element 503 is detachably connected to the sensing device 500. The use of detachable connections, such as screws or clips, facilitates the installation and removal of the sensing device 500.

[0080] During installation, the sealing element 503 can be connected to the sensing device 500 first, and then the whole assembly can be installed on the lower plate 102; alternatively, the sealing element 503 can be connected to the lower plate 102 first, and then the sensing device 500 can be connected to the sealing element 503.

[0081] Furthermore, the sensing device 500 includes a carrier plate 501 and a sensing chip 502 disposed on the carrier plate 501, the sensing chip 502 corresponding to the liquid channel 201 in the sensing area 301. In use, the liquid in the liquid channel 201 flows over the upper surface of the sensing chip 502 and is thus sensed by the sensing chip 502. A metal probe is disposed on the sensing chip 502, and the metal probe is electrically connected to a conductive component 404. The conductive component 404 then transmits the signal sensed by the sensing chip 502 to the outside. The metal probe can be of various forms, as long as it can be electrically connected to the conductive component 404; for example, it can be an electrode. The carrier plate 501 mainly serves to protect and fix the sensing chip 502. The carrier plate 501 can be a PCB board, and the carrier plate 501 and the sensing chip 502 are combined to form the entire sensing device 500.

[0082] Furthermore, the sealing element 503 is a sealing sheet, and a through hole is provided in the middle of the sealing sheet. The liquid channel 201 is connected to the sensing chip 502 through the through hole. The through hole in the middle of the sealing sheet allows liquid to flow into the through hole and reach the surface of the sensing chip 502 for sensing. The sensing area 301 can be effectively sealed by the sealing sheet to prevent leakage and the introduction of air bubbles.

[0083] Furthermore, the cross-sectional area of ​​the via is smaller than the upper surface area of ​​the sensing chip 502, and the edge of the via is located above the sensing chip 502. This ensures that the via is completely located above the sensing chip 502, meaning that the liquid inside the via only contacts the upper surface of the sensing chip 502, allowing it to be fully sensed.

[0084] In another preferred embodiment, the sealing sheet and the sensing device 500 are first installed in the cutout of the sealing area of ​​the lower plate 102 using bolts 1041. The rotary valve is adjusted to interrupt the liquid channel 201 and the flow channel. Then, the control valve at the fluid input port 103 is opened, and liquid is added through the fluid storage device 105 to allow the liquid to enter the liquid channel 201. The sensing chip 502 of the sensing device 500 continuously and stably senses the liquid in the sensing area 301. After sensing is completed, the rotary valve is adjusted to connect the liquid channel 201 and the flow channel. The sensed liquid then flows into the collection area 106. When there is too much liquid in the collection area 106, it can be discharged in time through the drain port 601.

[0085] like Figure 5 As shown, in another preferred embodiment, the fluid storage device 105 has a cylindrical structure, and the on / off device 500 is a rotary valve. The rotary valve is located on the upper surface of the upper plate 101, near the inlet of the collection area 106, and on one side of the liquid channel. The collection area 106 is a serpentine flow channel. The portion of the upper plate 101 within the sensing area 301 is recessed inward to form a spindle-shaped or rectangular groove. The sealing element 503 is a butterfly-shaped sealing sheet. The two wings of the sealing sheet are connected to the carrier plate 501 by bolts. The material of the sealing sheet is not specifically limited here, but its sealing performance should be the main consideration when selecting it. Both the sensing chip 502 and the carrier plate 501 are rectangular plate structures, and the carrier plate 502 is fixed in the middle. Three pairs of threaded holes are symmetrically arranged on the carrier plate 501 around the edge of the sensing chip 502. Among them, the middle pair of threaded holes are used to fix the sealing element 503, and the remaining threaded holes are used to bolt to the lower plate 102 to fix the carrier plate 501.

[0086] Both the upper plate 101 and the lower plate 102 have through screw holes at positions opposite to the other two pairs of threaded holes. During installation, the bolts are passed through the screw holes of the upper plate 101 and the lower plate 102 in sequence, and then screwed into the other two pairs of threaded holes on the carrier plate 501 to complete the installation and fixation of the sensing device 500.

[0087] like Figures 8-13As shown, in another preferred embodiment, the rotary valve has a rectangular structure and is fixed to the upper surface of the upper plate 101 by bolts. Two openings are provided through the upper plate 101 below the rotary valve, and the opening of the flow channel is located on the surface where the rotary valve contacts the upper plate 101. The arrows in the figure indicate the direction of liquid flow. When the rotary valve is in the "OFF" state, the bottom of the rotary valve will close the two openings, thereby interrupting the connection between the liquid channel 201 and the flow channel, that is, the liquid channel 201 is not connected to the collection area 106. At this time, continuous and stable sensing can be performed. When the rotary valve is in the "ON" state, the flow channel at the bottom of the rotary valve will connect with the two openings, thereby connecting the liquid channel 201 and the flow channel, that is, the liquid channel 201 is connected to the collection area 106. At this time, the liquid can enter the collection area 106 and be collected.

[0088] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. A microfluidic device, characterized in that, Includes a body and a liquid channel disposed within the body; The main body is provided with a sensing area; The body also has at least one hollow sealed chamber, and at least one of the sealed chambers is provided with a flow membrane. The sealed chamber and the liquid channel share the flow membrane. The sealed chamber is located upstream or downstream of the sensing area. The body includes an upper plate and a lower plate connected to each other, and the liquid channel is a cavity formed between the upper plate and the lower plate; wherein, the bottom of the liquid channel is part of the upper surface of the lower plate, and the top of the liquid channel is part of the lower surface of the upper plate.

2. The microfluidic device according to claim 1, characterized in that, The flow membrane is disposed at the top of the sealed chamber.

3. The microfluidic device according to claim 1, characterized in that, The main body is also provided with a fluid input port that communicates with the liquid channel, and the location of the fluid input port does not include the sensing area.

4. The microfluidic device according to claim 1, characterized in that, The portion of the upper plate within the sensing area is made of a transparent material.

5. The microfluidic device according to claim 1, characterized in that, The area of ​​the upper plate opposite to the sensing area is recessed towards the lower plate; the area of ​​the lower plate opposite to the sensing area is hollowed out.

6. The microfluidic device according to claim 1, characterized in that, The body also has a collection area inside, which is connected to the tail end of the liquid channel, and the collection area has a drain port.

7. The microfluidic device according to claim 6, characterized in that, Ventilation holes are also provided in the collection area.

8. The microfluidic device according to claim 1, characterized in that, The body is also provided with an on / off device, which is used to control the flow and interruption of liquid in the liquid channel.

9. The microfluidic device according to claim 1, characterized in that, The main body is also provided with at least one liquid inlet, which is connected to the sealed chamber.

10. The microfluidic device according to any one of claims 1-9, characterized in that, The bottom of the sealed chamber is provided with a sealing component or a conductive component, the conductive component being used to transmit electrical signals.

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