Microfluid device and microfluid detection device

Abstract

The invention discloses a microfluid device and a microfluid detection device. The microfluid device comprises a body (100) and a fluid channel (201) arranged in the body (100), the body (100) is further provided with a fluid input port (103) communicated with the fluid channel (201), and the arrangement position of the fluid input port (103) does not include the sensing area (301). The microfluid device and the microfluid detection device are convenient to operate, and can effectively solve the problem that bubbles are introduced due to the fact that a liquid injection hole is directly formed above the sensing area (301).

Description

[0001] This application is a divisional application of the original application with the original application number 202180101430.0, the original application date of December 10, 2021, and the original invention title of "Microfluidic device and microfluidic detection device". TECHNICAL FIELD

[0002] The present application relates to the technical field of microfluidics, in particular to a microfluidic device and a microfluidic detection device. BACKGROUND

[0003] Microfluidic technology is a technology for controlling, operating and detecting complex fluids at a microscale. It is a new cross-disciplinary subject developed on the basis of microelectronics, micromechanics, bioengineering and nanotechnology. In biological, chemical, material and other scientific experiments, fluid operations are often required, such as sample DNA preparation, liquid chromatography, PCR reaction, electrophoresis detection, etc. These operations are carried out in a liquid phase environment.

[0004] If the sample preparation, biochemical reaction, result detection and other steps are integrated on a biochip, the amount of fluid used in the experiment will usually be reduced from milliliters to microliters. At this time, a special microfluidic device is needed to meet the operation requirements. Microfluidic devices have the advantages of small size, small amount of sample / reagent used, fast reaction speed, etc. and are widely used in biological technology research.

[0005] However, the microfluidic device in the prior art usually has a liquid injection hole (as shown in FIG. 33) above the sensing area, which is used to add reagents or samples. This is easy to introduce air bubbles into the sensing area and is not easy to operate. Figure 1a And Figure 1b In view of the above, the present application is proposed.

[0006] In view of the above, the present application is proposed. SUMMARY

[0007] The present application provides a microfluidic device and a microfluidic detection device to solve the problem of air bubbles introduced by the microfluidic device in the prior art due to the liquid injection hole above the sensing area.

[0008] To solve the above problems and improve the integration of the biomolecular detection device and reduce the size of the detection device, the present application adopts the following scheme: A microfluidic device, comprising a body and a liquid channel arranged in the body; A sensing area is arranged on the body; A fluid input port in communication with the liquid channel is further arranged on the body, and the setting position of the fluid input port does not include the sensing area.

[0009] In the scheme, the microfluidic device comprises a body and a liquid channel arranged inside the body, the body is provided with a sensing area, and the liquid in the sensing area can be sensed as required; the body is further provided with a fluid input port in communication with the liquid channel, and the fluid input port is not arranged in the sensing area, that is, the fluid input port is not arranged directly above the sensing area; the to-be-detected liquid enters the liquid channel from the fluid input port and flows into the sensing area to be sensed, so that the operation is convenient, and the problem of air bubbles introduced due to the direct opening of the liquid injection hole above the sensing area can be effectively solved.

[0010] In a further preferred scheme, the body is further provided with an on-off device for controlling the flow and interruption of the liquid in the liquid channel. The liquid in the liquid channel is interrupted by the on-off device to stop flowing, so that the liquid in the sensing area is continuously and stably sensed, and the sensing efficiency is improved.

[0011] In a further preferred scheme, the on-off device is provided with a flow channel capable of communicating with the liquid channel; the on-off device controls whether the flow channel communicates with the liquid channel by relative movement with the body. When the on-off device is adjusted, the two ends of the flow channel in the on-off device communicate with the liquid channel, and the liquid normally flows; when the two ends of the flow channel in the on-off device do not communicate with the liquid channel, the on-off device interrupts the liquid in the liquid channel at this time.

[0012] In a further preferred scheme, the body is further provided with at least one hollow sealed chamber, and the top of at least one of the sealed chambers is provided with a flow-through membrane, and the sealed chamber and the liquid channel share the flow-through membrane. The liquid in the sealed chamber and the liquid in the liquid channel are independent of each other, and ion transmission or other forms of signal transmission between the two liquids can be achieved through the flow-through membrane.

[0013] In a further preferred scheme, the body is further provided with at least one liquid passage, and the liquid passage communicates with the sealed chamber. The sealed chamber can be filled with liquid through the liquid passage, and the number of liquid passages can be adjusted as required.

[0014] In a further preferred scheme, the bottom of the sealed chamber is provided with a sealing member or a conductive member, and the conductive member is used to transmit an electrical signal, and the sealing member is mainly used to close the bottom of the sealed chamber.

[0015] In a further preferred scheme, the sealed chamber comprises at least a cavity A and a cavity B in communication with each other, and at least one liquid inlet or outlet is arranged on the cavity A and the cavity B respectively, and the two liquid inlets and outlets cooperate with each other without interfering with each other.

[0016] In a further preferred scheme, the conductive member is arranged at the bottom of the cavity A and is used to transmit an electrical signal to the outside.

[0017] In a further preferred embodiment, the flow-through membrane is arranged on the top of the cavity B, and the bottom of the cavity B is provided with the sealing member. The flow-through membrane is used to realize ion transfer or other forms of signal transfer between the liquid in the cavity B and the liquid in the liquid channel; and the sealing member is used to seal the bottom of the cavity B to prevent the liquid from leaking out.

[0018] In a further preferred embodiment, the cavity A is in the shape of a shuttle and is arranged on one side of the liquid channel, the cavity B is arranged below the liquid channel, and the tip of the cavity A opposite to the liquid channel is in communication with the cavity B. The cavity B is arranged below the liquid channel, and the flow-through membrane arranged on the top of the cavity B can realize signal transfer with the liquid channel. The tip of the cavity A is in communication with the cavity B, thereby forming a sealed area as a whole and expanding the liquid storage capacity of the sealed area to a certain extent.

[0019] In a further preferred embodiment, a fluid storage device is arranged in communication with the fluid input port, and the top of the fluid storage device is open and the bottom of the fluid storage device is in communication with the fluid input port. Since the fluid input port of the microfluidic device is usually small in size, it is not convenient to add liquid directly through the fluid input port. In this embodiment, the fluid storage device can be used as a liquid adding device, and the top of the fluid storage device is large in size, thereby facilitating liquid addition.

[0020] In a further preferred embodiment, the fluid storage device is detachably arranged on the body, and the interior of the fluid storage device is in the shape of a funnel. The fluid storage device is detachably arranged on the body, thereby facilitating installation, disassembly and replacement. The interior of the fluid storage device is in the shape of a funnel, which is wide at the top and narrow at the bottom, thereby facilitating liquid filling.

[0021] In a further preferred embodiment, the area of the body opposite to the position of the sensing area is transparent, thereby facilitating observation of the state of the liquid in the sensing area.

[0022] In a further preferred embodiment, the body is further provided with a collection area, the collection area is in communication with the tail end of the liquid channel, and a liquid discharge port is arranged on the collection area. After the liquid in the liquid channel is sensed, the liquid can enter the collection area for collection. When the volume of the liquid in the collection area is greater than the volume of the collection area, the liquid can be discharged from the collection area through the liquid discharge port, thereby realizing the function of cyclic collection.

[0023] In a further preferred embodiment, the collection area is in the shape of a spiral or a snake. The spiral or snake-shaped structure can effectively increase the volume of the collection area, thereby facilitating collection of more liquid.

[0024] In a further preferred embodiment, the body comprises an upper plate and a lower plate connected to each other, and the liquid channel is a cavity formed between the upper plate and the lower plate. The bottom of the liquid channel is the upper surface of part of the lower plate, and the top of the liquid channel is the lower surface of part of the upper plate. The liquid channel is formed by the special structure of the upper plate and the lower plate, resources are fully utilized, and the structure of the upper plate and the lower plate also plays an important role in the whole microfluidic device.

[0025] In a further preferred scheme, the area opposite to the position of the sensing area of the upper plate is recessed towards the lower plate, facilitating observation of the state of the liquid in the sensing area; and the area opposite to the position of the sensing area of the lower plate is hollowed out, and the hollowed-out area is used to externally connect a sensing device.

[0026] In a further preferred scheme, the collection area is further provided with a vent hole. A negative pressure device can be externally connected at the vent hole, and the liquid in the fluid storage device is sucked into the liquid channel and the sensing area by applying negative pressure.

[0027] In a further preferred scheme, a control valve is arranged between the fluid input port and the fluid storage device, and the control valve is used to control whether the fluid input port is in communication with the fluid storage device. When the control valve is opened, the fluid input port is in communication with the fluid storage device, and at this time, the fluid storage device can be directly used to add liquid.

[0028] The present scheme also provides a microfluidic detection device comprising the above microfluidic device, and the microfluidic detection device further comprises: a sensing device connected to the body and corresponding to the sensing area; The sensing device and the liquid channel have an overlapping part, and the liquid in the liquid channel can flow through the overlapping part.

[0029] In the present scheme, the sensing device and the hollowed-out position of the sensing area correspond, and the sensing device and the liquid channel have an overlapping part, and the sensing device can be used to sense the liquid in the sensing area.

[0030] In a further preferred scheme, a sealing element is arranged between the sensing device and the body. The arrangement of the sealing element can effectively prevent liquid leakage at the connection between the sensing device and the body.

[0031] In a further preferred scheme, the sensing device is detachably connected to the lower plate, and the sealing element is detachably connected to the sensing device. The detachable connection facilitates installation and disassembly of the sensing device.

[0032] In a further preferred scheme, the sensing device comprises a carrier plate; and A sensing chip is arranged on the carrier plate, and the sensing chip corresponds to the liquid channel in the sensing area. In use, the liquid in the liquid channel flows through the upper surface of the sensing chip and is sensed by the sensing chip. The carrier plate mainly supports and fixes the sensing chip, and the carrier plate and the sensing chip are combined as a whole sensing device.

[0033] In a further preferred solution, the sealing element is a sealing sheet, and a through hole is arranged in the middle of the sealing sheet, and the liquid channel and the sensing chip are communicated through the through hole. The middle of the sealing sheet is provided with a through hole, and the liquid can flow into the through hole and reach the surface of the sensing chip to be sensed. The sensing area can be effectively sealed by the sealing sheet.

[0034] In a further preferred solution, the cross-sectional area of the through hole is smaller than the upper surface area of the sensing chip, and the edge of the through hole is located above the sensing chip. The through hole is completely located above the sensing chip, that is, the liquid in the through hole can be fully sensed by the upper surface of the sensing chip.

[0035] Compared with the prior art, the present application has the following beneficial effects: The present application provides a microfluidic device, which comprises a body and a liquid channel arranged in the interior of the body. The body is provided with a sensing area, and the fluid input port is not arranged directly above the sensing area. The liquid in the sensing area can be sensed as required. The liquid to be tested enters the liquid channel from the fluid input port and flows into the sensing area to be sensed. The operation is convenient, and the problem of introducing air bubbles caused by directly opening the liquid injection hole above the sensing area can be effectively solved.

[0036] The present application also provides a microfluidic detection device comprising the above-mentioned microfluidic device. The liquid to be detected can be directly sensed by the microfluidic detection device. The operation is convenient, and the practicability is strong. BRIEF DESCRIPTION OF DRAWINGS

[0037] In order to more clearly illustrate the specific embodiments of the present application or the technical solutions in the prior art, the drawings needed in the following specific embodiment or prior art description will be briefly introduced. Obviously, the drawings in the following description are some embodiments of the present application. Those skilled in the art can obtain other drawings without creative labor based on these drawings.

[0038] Figure 1a And Figure 1b The structure schematic diagram of the prior art microfluidic device described in the background art; Figure 2 The specific structure schematic diagram of the microfluidic device described in the present application; Figure 3 The Figure 2 The A-A sectional view in the above-mentioned embodiment. Figure 4 A specific structural exploded view of the microfluidic detection device according to the present application; Figure 5 Another angle of the structural exploded view of the microfluidic detection device according to the present application; Figure 6 Another specific structural schematic view of the microfluidic device according to the present application; Figure 7 A specific structural schematic view of the microfluidic device according to the present application; Figure 6 A cross-sectional view along B-B; Figure 8 A structural schematic view of the flow channel and the liquid channel of the on-off device according to the present application in the interrupted state; Figure 9 A cross-sectional view along C-C; Figure 8 A structural schematic view of the on-off device according to the present application; Figure 10 Figure 9 A cross-sectional view along D-D; Figure 11 A structural schematic view of the flow channel and the liquid channel of the on-off device according to the present application in the connected state; Figure 12 A cross-sectional view along E-E; Figure 11 A structural schematic view of the on-off device according to the present application; Figure 13 Figure 11 A cross-sectional view along F-F; Figure 14 A structural schematic view of the collection area according to the present application; Figure 15 Another structural schematic view of the collection area according to the present application.

[0039] In the above drawings, the components represented by the respective reference numerals are listed as follows: Body - 100; upper plate - 101; lower plate - 102; fluid input port - 103; on-off device - 104; fluid storage device - 105; collection area - 106; Liquid channel - 201; Sensing area - 301; Sealed chamber - 400; cavity A - 4001; cavity B - 4002; liquid passage - 401; flow-through membrane - 402; sealing component - 403; conductive component - 404; Sensing device - 500; carrier plate - 501; sensing chip - 502; sealing element - 503; Liquid discharge port - 601; air vent - 602. DETAILED DESCRIPTION

[0040] ​​In order to make the above and other features and advantages of the present application more comprehensible, the present application will be further described below with reference to the drawings. It is to be understood that the specific embodiments presented herein are by way of example only and are not limiting as to the scope of the present application.

[0041] In the description of the present application, it is to be understood that the orientations or positional relationships indicated by the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like are based on the orientations or positional relationships shown in the drawings, and are merely intended to facilitate the description of the present application and simplify the description, and therefore cannot be understood as indicating or implying that the devices or elements indicated must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0042] In addition, the terms "first", "second", "third", etc. are used only for the purpose of description and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features defined with "first", "second", etc. can explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "a plurality of" is at least two, such as two, three, etc., unless otherwise explicitly specified and limited.

[0043] In the present application, unless otherwise explicitly specified and limited, the terms "mounting", "connection", "connecting", "fixing", and the like should be understood broadly, for example, can be fixed connection, can also be detachable connection, or integral; can be mechanical connection, can also be electrical connection; can be direct connection, can also be indirect connection through an intermediate medium, can be the internal communication of two elements or the interaction relationship between two elements, unless otherwise explicitly limited. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0044] In the present application, unless otherwise explicitly specified and limited, the first feature "on" or "under" the second feature can be that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature "above", "over" and "on" the second feature can be that the first feature is directly above or obliquely above the second feature, or only indicates that the horizontal height of the first feature is higher than that of the second feature. The first feature "below", "under" and "under" the second feature can be that the first feature is directly below or obliquely below the second feature, or only indicates that the horizontal height of the first feature is less than that of the second feature.

[0045] In the description of the present specification, the description referring to the terms "one embodiment", "some embodiments", "an example", "a specific example", or "some examples" and the like means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples. In addition, the person skilled in the art can combine and combine the different embodiments or examples described in the present specification and the features of the different embodiments or examples without contradiction.

[0046] Please refer to Figures 2-7 As shown in the drawings, the present application provides a microfluidic device, comprising a body 100 and a liquid channel 201 arranged in the body 100; the body 100 is provided with a sensing area 301; the body 100 is further provided with a fluid input port 103 in communication with the liquid channel 201, and the setting position of the fluid input port 103 does not include the sensing area 301.

[0047] In this scheme, the microfluidic device comprises a body 100 and a liquid channel 201 arranged in the interior of the body 100, the body 100 is provided with a sensing area 301, the liquid in the liquid channel 201 can flow through the sensing area 301, and the liquid in the sensing area 301 can be sensed as needed; The body 100 is further provided with a fluid input port 103 in communication with the liquid channel 201, and the setting position of the fluid input port 103 does not include the sensing area 301, that is, the fluid input port 103 is not arranged directly above the sensing area 301, which can effectively solve the problem of introducing air bubbles due to directly opening the liquid injection hole above the sensing area.

[0048] Further, the body 100 is further provided with a on-off device 104, which is used to control the flow and interruption of the liquid in the liquid channel 201. By using the on-off device 104 to interrupt the liquid in the liquid channel 201, the liquid is stopped flowing, so as to ensure that the liquid in the sensing area 301 is continuously and stably sensed, and the sensing efficiency is improved.

[0049] It should be noted that the on-off device 104 is mainly used to control the flow and interruption of the liquid in the liquid channel 201, and its specific structure and working principle are not limited here.

[0050] Furthermore, the switching device 104 is provided with a flow channel that can communicate with the liquid channel 201. The switching device 104 controls whether the flow channel and the liquid channel 201 are connected by relative movement with the body 100. When the switching device 104 is adjusted so that both ends of the flow channel in the switching device 104 are connected with the liquid channel 201, the liquid flows normally; when both ends of the flow channel in the switching device 104 are not connected with the liquid channel 201, the switching device 104 will interrupt the liquid in the liquid channel 201. In this solution, the manner in which the switching device 104 and the body 100 move relative to each other is not specifically limited, such as translation or rotation.

[0051] In another preferred embodiment, the on / off device 104 is a rotary valve, which has a flow channel that can communicate with the liquid channel 201. Rotating the rotary valve controls whether the flow channel and the liquid channel 201 are connected. Figures 8-10 As shown, when the rotary valve is in the "OFF" state, the flow channel and liquid channel 201 are disconnected, allowing for continuous and stable sensing of the liquid within the sensing area 301; Figures 11-13 As shown, when the rotary valve is in the "ON" state, the flow channel and the liquid channel 201 are in a connected state. At this time, the liquid in the liquid channel 201 can flow smoothly through the flow channel and enter the collection area 106 to be collected.

[0052] Furthermore, such as Figure 6 and Figure 7 As shown, the main body 100 also includes at least one hollow sealed chamber 400, and a flow membrane 402 is disposed on the top of at least one sealed chamber 400. The sealed chamber 400 and the liquid channel 201 share the flow membrane 402, that is, the sealed chamber 400 and the liquid channel 201 are separated into two independent spaces by the flow membrane 402. The liquid in the sealed chamber 400 and the liquid in the liquid channel 201 are independent of each other, and ion transfer or other forms of signal transfer can be achieved between the liquid in the sealed chamber 400 and the liquid in the liquid channel 201 through the flow membrane 402. The specific structure and material of the flow membrane 402 are not limited here, and can be made according to the actual functional requirements. For example, in some cases, the flow membrane 402 can be a semi-permeable membrane.

[0053] It should be understood that the sealed chamber 400 in this solution only needs to be located between the on / off device 104 and the fluid input port 103, but it can be located upstream or downstream of the sensing area 301.

[0054] Furthermore, the main body 100 is also provided with at least one liquid inlet 401, which communicates with the sealed chamber 400. Liquid can be added to the sealed chamber 400 through the liquid inlet 401, and the number of liquid inlets 401 can be adjusted as needed. In this solution, the liquid inlet 401 can be used both to add liquid to the sealed chamber 400 and as a discharge channel for liquid in the sealed chamber 400.

[0055] Further, as shown in Figure 7 the bottom of the sealed chamber 400 is provided with a sealing member 403 or a conductive member 404 for transmitting electrical signals, the specific form and the position of the sealing member 403 are not limited in the embodiment, and the sealing member 403 is mainly used for closing the bottom of the sealed chamber 400 to avoid liquid extravasation.

[0056] Further, as shown in Figure 4 the sealed chamber 400 at least includes a cavity A 4001 and a cavity B 4002 in communication with each other, and at least one liquid inlet / outlet port 401 is provided on the cavity A 4001 and the cavity B 4002 respectively. When the liquid inlet / outlet port 401 on the cavity A 4001 is used for liquid inlet, the liquid inlet / outlet port 401 on the cavity B 4002 is used for liquid outlet; similarly, when the liquid inlet / outlet port 401 on the cavity B 4002 is used for liquid inlet, the liquid inlet / outlet port 401 on the cavity A 4001 is used for liquid outlet, and the two liquid inlet / outlet ports 401 do not interfere with each other. The position and form of the cavity A 4001 and the cavity B 4002 can be arranged according to the needs of detection.

[0057] Further, the conductive member 404 is arranged at the bottom of the cavity A 4001 and used for transmitting electrical signals to the outside. In another embodiment, the conductive member 404 can be a metal sheet or an electrode.

[0058] Further, the flow-through membrane 402 is arranged at the top of the cavity B 4002, and the sealing member 403 is arranged at the bottom of the cavity B 4002. The flow-through membrane 402 is used for realizing ion transmission or other forms of signal transmission between the liquid in the cavity B 4002 and the liquid in the liquid channel 201; and the sealing member 403 is used for closing the bottom of the cavity B 4002 to prevent liquid extravasation. In another embodiment, the sealing member 403 can be a sheet-shaped object.

[0059] Since the cavity A 4001 and the cavity B 4002 are in communication with each other and are close to each other, the conductive member 404 is arranged at the bottom of the cavity A 4001, and the flow-through membrane 402 is arranged at the top of the cavity B 4002, so that the conductive member 404 and the flow-through membrane 402 can be soaked by the same liquid.

[0060] Further, the cavity A 4001 is in the shape of a shuttle and is located at one side of the liquid channel 201, and the cavity B 4002 is located below the liquid channel 201, and the tip of the cavity A 4001 opposite to the liquid channel 201 is communicated with the cavity B 4002. The cavity B 4002 is located below the liquid channel 201, and the cavity B 4002 can realize signal transmission with the liquid channel 201 through the flow membrane 402 arranged at the top of the cavity B 4002, that is, the liquid in the cavity B 4002 and the liquid in the liquid channel 201 can realize ion transmission or other forms of signal transmission through the flow membrane 402, and the cavity A 4001 is communicated with the cavity B 4002 to form a sealed area as a whole.

[0061] It should be understood that the structure of the cavity A 4001 and the cavity B 4002 can be adjusted as needed, and in the present scheme, the cavity A 4001 is arranged in the shape of a shuttle, which is only one embodiment, and in other embodiments, the cavity A 4001 can also be in the shape of an ellipse or other shapes. The cavity A 4001 and the cavity B 4002 are detachably arranged in the body 100, and whether to be installed or only one of them to be installed can be selected as needed.

[0062] Further, the fluid storage device 105 is arranged in cooperation with the fluid input port 103, the top end of the fluid storage device 105 is open, and the bottom end is communicated with the fluid input port 103. Since the fluid input port 103 of the microfluidic device is usually small in size, it is not convenient to directly add liquid through the fluid input port 103, and in the present scheme, the fluid storage device 105 can be used as a liquid adding device, and the top end is large in size, which is convenient for adding liquid and is not easy to spill.

[0063] Further, the fluid storage device 105 is detachably arranged on the body 100, and the inside of the fluid storage device 105 is in the shape of a funnel. The fluid storage device 105 is detachably arranged on the body 100, which is convenient for installation, disassembly and replacement, and in order to facilitate the liquid to fully enter the liquid channel 201, the inside of the fluid storage device 105 is arranged in the shape of a funnel, which is wide at the top and narrow at the bottom, so as to facilitate the liquid to be filled, and the liquid can be prevented from being left on the inner wall of the fluid storage device 105 as much as possible.

[0064] It should be noted that the fluid storage device 105 can be made into different shapes as needed, and the above-mentioned funnel-shaped structure is only a more preferred embodiment. The shape of the fluid input port 103 can also be adjusted according to actual needs, such as a round port, a square port or a horn port.

[0065] Further, the area of the body 100 opposite to the position of the sensing area 301 is transparent, so as to facilitate the observation of the liquid state in the sensing area 301. Figure 2The middle circle only refers to the approximate range of the sensing area 301, and the body 100 in the area can be made of a transparent material to visually observe the liquid state in the sensing area 301 and improve the sensing efficiency. The shape of the sensing area 301 is not limited and can be adjusted according to actual needs.

[0066] Further, the body 100 is further provided with a collection area 106, which is in communication with the tail end of the liquid channel 201, and a liquid discharge port 601 is formed on the collection area 106. After the liquid in the liquid channel 201 is sensed, the on-off device 104 is opened, so that the liquid in the liquid channel 201 can enter the collection area 106 for collection. When the volume of the liquid in the collection area 106 is greater than the volume of the collection area 106, the liquid can be discharged from the collection area 106 through the liquid discharge port 601, such as using a syringe to draw the liquid from the liquid discharge port 601, so that the collection area 106 can continue to collect liquid to realize the function of cyclic collection.

[0067] Further, as shown in Figure 14 and Figure 15 , the collection area 106 is in a spiral or serpentine structure. The spiral or serpentine structure can effectively increase the volume of the collection area 106 and facilitate the collection of more liquid.

[0068] It should be noted that the form of the liquid collection area 106 can be various, which can be a liquid storage pool or other storage media such as water-absorbing cotton, and the collection area 106 is not limited to a cavity structure.

[0069] Further, as shown in Figure 4 , the body 100 includes an upper plate 101 and a lower plate 102 connected to each other, and the liquid channel 201 is a cavity formed between the upper plate 101 and the lower plate 102. Among them, the bottom of the liquid channel 201 is the upper surface of part of the lower plate 102, and the top of the liquid channel 201 is the lower surface of part of the upper plate 101. In specific use, the upper plate 101 and the lower plate 102 are permanently combined together by welding, gluing, bonding or the like, and the liquid channel 201 is formed by the special structure of the upper plate 101 and the lower plate 102, which fully utilizes resources, and the structure of the upper plate 101 and the lower plate 102 also plays an important role in the whole microfluidic device.

[0070] It should be understood that the part of the upper plate 101 in the sensing area 301 is made of a transparent material in the present scheme.

[0071] Further, the region opposite to the position of the sensing area 301 of the upper plate 101 is recessed towards the direction of the lower plate 102, i.e. the thickness of the upper plate 101 at the sensing area 301 is reduced, which facilitates the observation of the state of the liquid in the sensing area 301; the region opposite to the position of the sensing area 301 of the lower plate 102 is hollowed, and the form and size of the hollowed region can be adjusted as needed, and the hollowed region is used to externally connect the sensing device 500.

[0072] Further, the collection area 106 is further provided with a vent hole 602. A negative pressure device can be externally connected at the vent hole 602, and the liquid in the fluid storage device 105 is sucked into the liquid channel 201 and the sensing area 301 by applying negative pressure.

[0073] It should be noted that the liquid enters the liquid channel 201 from the fluid inlet port 103 in the form of negative pressure applied downstream, or in the form of external pressure, or by pre-venting the air in the liquid channel 201 to form a vacuum chamber for self-suction of the fluid.

[0074] Further, a control valve is arranged between the fluid inlet port 103 and the fluid storage device 105, and the control valve is used to control whether the fluid inlet port 103 is in communication with the fluid storage device 105. When the control valve is opened, the fluid inlet port 103 is in communication with the fluid storage device 105, and at this time the fluid storage device 105 can be directly used to add liquid; when the control valve is closed, the fluid inlet port 103 is cut off from the fluid storage device 105, and the liquid cannot flow into the fluid inlet port 103.

[0075] As shown in Figure 5 The present scheme further provides a microfluidic detection device comprising the microfluidic device, and the microfluidic detection device further comprises: a sensing device 500 connected to the body 100 and corresponding to the sensing area 301; The sensing device 500 overlaps with the liquid channel 201 and can allow the liquid in the liquid channel 201 to flow through.

[0076] In the present scheme, the sensing device 500 corresponds to the hollowed position of the sensing area 301, and the sensing device 500 overlaps with the liquid channel 201, and the liquid in the sensing area 301 can be sensed by the sensing device 500.

[0077] In use, the sensing device 500 is sealingly installed at the hollowed position of the sensing area 301, so that the liquid channel 201 at this position is in a sealed state, and the sensing device 500 serves as a part of the liquid channel 201, and the liquid flowing through the surface of the sensing device 500 can be effectively sensed.

[0078] Further, a sealing element 503 is arranged between the sensing device 500 and the body 100. The sealing element 503 is arranged to keep the sensing area 301 in a sealed state, which can effectively avoid liquid leakage at the connection between the sensing device 500 and the body 100, and can also avoid the introduction of air bubbles, thereby improving the sensing efficiency.

[0079] Further, the sensing device 500 is detachably connected with the lower plate 102, and the sealing element 503 is detachably connected with the sensing device 500. The detachable connection mode, such as through screws or buckles, facilitates the installation and disassembly of the sensing device 500.

[0080] In particular, during installation, the sealing element 503 and the sensing device 500 can be connected together, and then the whole is installed on the lower plate 102; or the sealing element 503 and the lower plate 102 can be connected together, and then the sensing device 500 is connected with the sealing element 503.

[0081] Further, the sensing device 500 includes a carrier plate 501, and a sensing chip 502 arranged on the carrier plate 501, the sensing chip 502 corresponding to the liquid channel 201 in the sensing area 301. During use, the liquid in the liquid channel 201 flows through the upper surface of the sensing chip 502, and is sensed by the sensing chip 502. The sensing chip 502 is provided with metal probes, the metal probes are electrically connected with the conductive part 404, and the conductive part 404 transmits the signal sensed by the sensing chip 502 to the outside. The metal probes can be various, as long as they can be electrically connected with the conductive part 404, for example, they can be electrodes. The carrier plate 501 mainly plays a role of protecting and fixing the sensing chip 502. The carrier plate 501 can be a PCB board, and the carrier plate 501 and the sensing chip 502 are combined as a whole sensing device 500.

[0082] Further, the sealing element 503 is a sealing sheet, and a through hole is arranged in the middle of the sealing sheet, and the liquid channel 201 and the sensing chip 502 are communicated through the through hole. The through hole is arranged in the middle of the sealing sheet, and the liquid can flow into the through hole and reach the surface of the sensing chip 502 to be sensed. The sensing area 301 can be effectively sealed by the sealing sheet to avoid leakage and the introduction of air bubbles.

[0083] Further, the cross-sectional area of the through hole is smaller than the upper surface area of the sensing chip 502, and the edge of the through hole is located above the sensing chip 502. The through hole is completely located above the sensing chip 502, that is, the liquid in the through hole only contacts the upper surface of the sensing chip 502, which can be fully sensed.

[0084] In another preferred embodiment, the sealing sheet and the sensing device 500 are first installed in the cutout of the sealing area of ​​the lower plate 102 using bolts 1041. The rotary valve is adjusted to interrupt the liquid channel 201 and the flow channel. Then, the control valve at the fluid input port 103 is opened, and liquid is added through the fluid storage device 105 to allow the liquid to enter the liquid channel 201. The sensing chip 502 of the sensing device 500 continuously and stably senses the liquid in the sensing area 301. After sensing is completed, the rotary valve is adjusted to connect the liquid channel 201 and the flow channel. The sensed liquid then flows into the collection area 106. When there is too much liquid in the collection area 106, it can be discharged in time through the drain port 601.

[0085] like Figure 5 As shown, in another preferred embodiment, the fluid storage device 105 has a cylindrical structure, and the on / off device 500 is a rotary valve. The rotary valve is located on the upper surface of the upper plate 101, near the inlet of the collection area 106, and on one side of the liquid channel. The collection area 106 is a serpentine flow channel. The portion of the upper plate 101 within the sensing area 301 is recessed inward to form a spindle-shaped or rectangular groove. The sealing element 503 is a butterfly-shaped sealing sheet. The two wings of the sealing sheet are connected to the carrier plate 501 by bolts. The material of the sealing sheet is not specifically limited here, but its sealing performance should be the main consideration when selecting it. Both the sensing chip 502 and the carrier plate 501 are rectangular plate structures, and the carrier plate 502 is fixed in the middle. Three pairs of threaded holes are symmetrically arranged on the carrier plate 501 around the edge of the sensing chip 502. Among them, the middle pair of threaded holes are used to fix the sealing element 503, and the remaining threaded holes are used to bolt to the lower plate 102 to fix the carrier plate 501.

[0086] Both the upper plate 101 and the lower plate 102 have through screw holes at positions opposite to the other two pairs of threaded holes. During installation, the bolts are passed through the screw holes of the upper plate 101 and the lower plate 102 in sequence, and then screwed into the other two pairs of threaded holes on the carrier plate 501 to complete the installation and fixation of the sensing device 500.

[0087] like Figures 8-13In another preferred embodiment, the rotary valve is in a rectangular structure, which is fixed on the upper surface of the upper plate 101 by bolts, two openings are provided on the upper plate 101 below the rotary valve, the openings of the flow channel are provided on the surface of the rotary valve in contact with the upper plate 101, and the direction of the arrows in the figure indicates the direction of the liquid flow. When the rotary valve is in the "OFF" state, the bottom of the rotary valve will close the two openings, so as to cause the liquid channel 201 to be interrupted with the flow channel, that is, the liquid channel 201 is not communicated with the collection area 106, at this time, continuous and stable sensing can be carried out; when the rotary valve is in the "ON" state, the flow channel at the bottom of the rotary valve will be communicated with the two openings, so as to cause the liquid channel 201 to be communicated with the flow channel, that is, the liquid channel 201 is communicated with the collection area 106, at this time, the liquid can enter the collection area 106 to be collected.

[0088] Although the embodiments of the present application have been shown and described above, it should be understood that the above-mentioned embodiments are exemplary and should not be construed as limiting the present application, and those skilled in the art can make changes, modifications, replacements and variations to the above-mentioned embodiments within the scope of the present application.

Claims

1. A microfluidic device, characterized in that, Includes a body and a liquid channel disposed within the body; The main body is provided with a sensing area; The main body is also provided with a fluid input port that communicates with the liquid channel; The body also includes at least one hollow sealed chamber, and at least one of the sealed chambers is provided with a flow membrane. The sealed chamber and the liquid channel share the flow membrane. The sealed chamber is located upstream or downstream of the sensing area.

2. The microfluidic device according to claim 1, characterized in that, The flow membrane is disposed at the top of the sealed chamber.

3. The microfluidic device according to claim 1, characterized in that, The location of the fluid input port does not include the sensing area.

4. The microfluidic device according to claim 1, characterized in that, The body is also provided with an on / off device, which is used to control the flow and interruption of liquid in the liquid channel.

5. The microfluidic device according to claim 4, characterized in that, The switching device is provided with a flow channel that can communicate with the liquid channel; the switching device controls whether the flow channel and the liquid channel are connected by relative movement with the main body.

6. The microfluidic device according to claim 1, characterized in that, The main body is also provided with at least one liquid inlet, which is connected to the sealed chamber.

7. The microfluidic device according to claim 1, characterized in that, The bottom of the sealed chamber is provided with a sealing component or a conductive component, the conductive component being used to transmit electrical signals.

8. The microfluidic device according to claim 1, characterized in that, The area on the body that is opposite to the sensing area is transparent.

9. The microfluidic device according to claim 1, characterized in that, The flow membrane is a semi-permeable membrane.

10. The microfluidic device according to any one of claims 1-9, characterized in that, The body also has a collection area inside, which is connected to the tail end of the liquid channel, and a drain port is provided on the collection area.

11. A microfluidic device, characterized in that, Includes a body and a liquid channel disposed within the body; The main body is provided with a sensing area; The main body is also provided with a fluid input port that communicates with the liquid channel, and the location of the fluid input port does not include the sensing area.

12. The microfluidic device according to claim 11, characterized in that, The body is also provided with an on / off device, which is used to control the flow and interruption of liquid in the liquid channel.

13. The microfluidic device according to claim 12, characterized in that, The switching device is provided with a flow channel that can communicate with the liquid channel; the switching device controls whether the flow channel and the liquid channel are connected by relative movement with the main body.

14. The microfluidic device according to claim 13, characterized in that, The body also includes at least one hollow sealed chamber, and a flow membrane is provided on the top of at least one of the sealed chambers, the sealed chamber and the liquid channel sharing the flow membrane.

15. The microfluidic device according to claim 14, characterized in that, The main body is also provided with at least one liquid inlet, which is connected to the sealed chamber.

16. The microfluidic device according to claim 15, characterized in that, The bottom of the sealed chamber is provided with a sealing component or a conductive component, the conductive component being used to transmit electrical signals.

17. The microfluidic device according to claim 11, characterized in that, A fluid storage device is connected to the fluid input port. The top of the fluid storage device is open, and the bottom is connected to the fluid input port.

18. The microfluidic device according to claim 11, characterized in that, The area on the main body opposite to the sensing area is transparent, and a collection area is also provided inside the main body. The collection area is connected to the tail end of the liquid channel, and a drain port is provided on the collection area.

19. The microfluidic device according to any one of claims 11-18, characterized in that, The body includes an upper plate and a lower plate that are connected to each other, and the liquid channel is a cavity formed between the upper plate and the lower plate; The bottom of the liquid channel is part of the upper surface of the lower plate, and the top of the liquid channel is part of the lower surface of the upper plate.

20. The microfluidic device according to claim 19, characterized in that, The area of ​​the upper plate opposite to the sensing area is recessed towards the lower plate; the area of ​​the lower plate opposite to the sensing area is hollowed out.

21. The microfluidic device according to claim 17, characterized in that, A control valve is provided between the fluid input port and the fluid storage device, and the control valve is used to control whether the fluid input port and the fluid storage device are connected.

22. A microfluidic detection device comprising the microfluidic device according to any one of claims 11-21, characterized in that, Also includes: A sensing device connected to the body and corresponding to the sensing area; The sensing device overlaps with the liquid channel, and the liquid in the liquid channel can flow through it.

23. The microfluidic detection device according to claim 22, characterized in that, A sealing element is provided between the sensing device and the body; The sensing device is detachably connected to the lower plate; the sealing element is detachably connected to the sensing device.

24. The microfluidic detection device according to claim 22 or 23, characterized in that, The sensing device includes a carrier plate; and A sensing chip is disposed on the carrier plate, and the sensing chip corresponds to a liquid channel in the sensing area.

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