Use of charged air ions in cosmetic treatment

Abstract

The present invention relates to methods and cosmetic treatment methods for treating skin conditions using non-contact electrical stimulation (ES), which are used as means to achieve skin rejuvenation and general and specific improvements in skin appearance. In a preferred embodiment, ES is achieved by applying ionized gas ions. Another embodiment relates to a combination of a non-contact ES and topical application of a cosmetic or pharmaceutical substance, the effect of which is enhanced by the non-contact ES.

Description

Technical Field

[0001] This invention relates to the field of cosmetic treatments. In particular, it relates to the use of non-contact electrical stimulation (ES) as a means of achieving skin rejuvenation and general and specific improvements in skin appearance. The invention also relates to combinations of non-contact ES and topical application of cosmetic or pharmaceutical substances. Background Technology

[0002] Electrical stimulation (also known as electrical stimulation or ES) is a well-known technique that has been used to treat a wide range of pathologies—when used in medical treatment, ES is often referred to as electrotherapy.

[0003] Traditionally, ES involves transmitting current through an applied surface electrode pad that is in electrolytic contact with the external skin surface and the area receiving the effect. In ES, at least two electrodes (a lead and a discharge electrode) are required to complete the circuit.

[0004] ES has been used as a treatment for many conditions, especially for various types of wounds, such as acute wounds, burns, and chronic wounds, including pressure ulcers, diabetic ulcers, venous ulcers, and arterial ulcers.

[0005] A more recent development, detailed in WO 2009 / 135896, replaces the use of applied electrodes with the application of airborne ions from a non-contact device. Typically, using a device such as that disclosed in WO 2007 / 042029, an electric current / charge is delivered to the anatomical area being treated via negatively charged O2 ions. This method offers higher patient compliance, both because the application of air ions is painless and because it reduces the risk of wound infection. A later development, disclosed in WO 2015 / 052170, combines the application of gaseous ions with phototherapy.

[0006] In the field of cosmetic treatments, traditional methods for rejuvenation rely on the use of cosmetic compositions that seek to maintain or improve skin homeostasis through their contents—namely, the content of nourishing, moisturizing, and keratin-reducing compounds and substances. A different set of methods utilizes invasive (even surgical) procedures to apply fillers or tighten the skin, particularly in the facial area.

[0007] Purpose of the invention

[0008] The purpose of embodiments of the present invention is to provide a non-invasive and non-pharmacological alternative to existing cosmetic treatments. Another purpose is to provide a method for improving the non-invasive delivery of cosmetic active compounds to the skin. Summary of the Invention

[0009] The inventors have discovered that applying a gas ion-induced microcurrent can deliver current to muscle and skin cells. This microcurrent builds muscle and / or lifts and tightens skin tissue in the face. Furthermore, the establishment of the microcurrent also helps deliver active compounds commonly used in cosmetic treatments to the lower layers of the skin, thereby enhancing their beneficial effects.

[0010] Therefore, in a first aspect, the present invention relates to a method for cosmetic treatment of a surface area of ​​skin for a human subject, the method comprising projecting ionized gas molecules onto the area of ​​skin to establish a current driven by the projection of the ionized gas molecules in or above the area of ​​skin, wherein the human subject optionally has at least one discharge electrode attached to a body area remote from the surface area of ​​the skin in order to maintain the current.

[0011] In a second aspect, the present invention relates to ionized gas molecules in a method for treating a region of human skin, the method being selected from redness relief, sensitivity relief, acne reduction, prevention or repair of sunburn and / or photodamage, anti-inflammatory treatment of the skin, treatment to improve the effect of laser treatment, treatment to improve the effect of cryolipolysis, and reduction of postoperative scarring and pigmentation, wherein the method includes projecting ionized gas molecules onto a region of skin to establish a current driven by the projection of the ionized gas molecules in or above the region of skin, wherein the human subject optionally has at least one discharge electrode attached to a body region remote from the surface region of the skin to maintain the current.

[0012] In a third aspect, the present invention relates to a method for therapeutic treatment of a surface area of ​​skin for a human subject, the method being carried out by topically applying an active pharmaceutical substance or a composition comprising said substance to the skin, the method comprising projecting ionized gas molecules onto the area of ​​skin to establish a current driven by the projection of the ionized gas molecules in or above the area of ​​skin, wherein the human subject optionally has at least one discharge electrode attached to a region of the body remote from the surface area of ​​the skin to maintain said current, and wherein the projection of the ionized gas molecules enhances the absorption and / or therapeutic effect of the applied active pharmaceutical substance or composition. A portion of the third aspect relates to the same basic principles, but in a non-therapeutic setting, and thus provides a method for non-therapeutic cosmetic treatment of a surface area of ​​skin for a human subject, the method being carried out by topically applying a cosmetic active substance or a composition containing said active substance to the skin, the method comprising projecting ionized gas molecules onto the area of ​​skin to establish a current driven by the projection of ionized gas molecules in or above the area of ​​skin, wherein the human subject optionally has at least one discharge electrode attached to a region of the body remote from the surface area of ​​the skin to maintain said current, and wherein the projection of ionized gas molecules enhances the absorption and / or cosmetic effect of the applied active cosmetic substance or composition. Detailed Implementation

[0013] definition

[0014] "Projection of ionized gas molecules" means providing a sufficient charge in the form of charged gas molecules to a surface area on the skin to allow an electric current to be established between the surface area and the surrounding tissue. It should be emphasized that the current transmitted from the surface area to the surrounding tissue cannot be precisely determined—when discussed in quantitative terms in this application, the quantity (measured in amperes or microamperes) is a quantity that can be measured in one or more discharge electrodes that return the current from the treated human to the device delivering the ionized gas molecules.

[0015] "Ionized gas molecules" broadly refers to gas molecules that carry a net charge. Typical examples are negatively charged oxygen molecules and positively charged nitrogen molecules, due to their abundance in the atmosphere. However, any gas molecule capable of carrying a net charge is useful in the practice of this invention, provided that such ionized gas molecules do not produce unacceptable side effects or toxicity to the human subjects being treated.

[0016] A "discharge electrode" is an electrode attached to the subject being treated, ensuring that ionized gas molecules do not simply build up an electrostatic charge in the subject, but rather create a current that travels through the subject to the discharge electrode. Typically, such an electrode would be in the form of a strip, but any convenient physical form can be useful. A particular example of a discharge electrode is a "return electrode," which (optionally via a control unit) is connected to the device supplying ionized gas ions. This allows for the measurement of the return current (see above), and in turn, enables complete control over the current transmitted through the subject due to the delivery of ionized gas molecules.

[0017] A "gas ion transport device" is an apparatus or device capable of generating a flow, jet, or flux of ionized gas molecules having a net positive or negative charge, wherein the direction of the flow, jet, or flux can be optionally controlled with respect to dosage, intensity, and orientation. Details of such devices are discussed in the patent application publications cited in the aforementioned background section.

[0018] Specific embodiments of the present invention

[0019] Typically, the projection of gas ions (and optionally light) onto a skin surface area can be performed as described in WO 2009 / 135896 and WO 2015 / 052170, where the treatment is tailored to various pathological conditions. However, the details disclosed in these patent publications regarding the timing of the treatment, the dosage of gas ions (in terms of applied µA), the offset of the current direction, and the charge of ionized gas molecules are adapted to the present invention with the necessary modifications.

[0020] First aspect of the present invention and its embodiments

[0021] A first aspect of the invention relates to a method for cosmetic treatment of a surface area of ​​skin of a human subject, the method comprising projecting ionized gas molecules onto the area of ​​skin to establish a current driven by the projection of the ionized gas molecules in or above the area of ​​skin, wherein the human subject optionally has at least one discharge electrode attached to a region of the body remote from the surface area of ​​the skin in order to maintain the current.

[0022] The charge of ionized gas molecules can be either negative or positive, depending on the choice of device used to generate air ions. However, in a preferred embodiment, the ionized gas molecules are predominantly negatively charged, such as negatively charged oxygen molecules (O2). - (However, as mentioned above, ionized gas molecules can alternatively be predominantly positively charged, such as positively charged nitrogen molecules (N2).) + In any case, in most practical embodiments of the invention, the ionized gas molecules originate from the atmosphere.

[0023] The projection of ionized gas molecules preferably establishes an electric current between the surface area of ​​the skin and the underlying tissue.

[0024] When treating subjects, the current is typically at least 0.1 μA and at most 100 µA. However, any ampere number within this range can be used, such as approximately 0.2 µA, approximately 0.3 µA, approximately 0.4 µA, approximately 0.5 µA, approximately 0.6 µA, approximately 0.7 µA, approximately 0.8 µA, approximately 0.9 µA, approximately 1 µA, approximately 2 µA, approximately 3 µA, approximately 4 µA, approximately 5 µA, approximately 6 µA, approximately 7 µA, approximately 8 µA, approximately 9 µA, approximately 10 µA, approximately 11 µA, approximately 12 µA, approximately 13 µA, approximately 14 µA, approximately 15 µA, approximately 16 µA, approximately 17 µA, approximately 18 µA, approximately 19 µA, approximately 20 µA, approximately 21 µA, approximately 22 µA, approximately 23 µA, approximately 24 µA, approximately 25 µA, approximately 26 µA, approximately 27 µA, approximately 28 µA, approximately 29 µA, approximately 30 µA, approximately 31 µA, approximately 32 µA. µA, about 33 µA, about 34 µA, about 35 µA, about 36 µA, about 37 µA, about 38 µA, about 39 µA, about 40 µA, about 41 µA, about 42 µA, about 43 µA, about 44 µA, about 45 µA, about 46 µA, about 47 µA, about 48 µA, ~49 µA, ~50 µA, ~51 µA, ~52 µA, ~53 µA, ~54 µA, ~55 µA, ~56 µA, ~57 µA, ~58 µA, ~59 µA, ~60 µA, ~61 µA, ~62 µA, ~63 µA, ~64 µA, ~65 µA, ~66 µA, ~67 µA, ~68 µA, ~69 µA, ~70 µA, ~71 µA, ~72 µA, ~73 µA, about 74 µA, about 75 µA, about 76 µA, about 77 µA, about 78 µA, about 79 µA, about 80 µA, about 81 µA, about 82 µA, about 83 µA, about 84 µA, about 85 µA, about 86 µA, about 87 µA, about 88 µA, about 89 µA, ~90 µA, ~91 µA, ~92 µA, ~93 µA, ~94 µA, ~95 µA, ~96 µA, ~97 µA, ~98 µA, and ~99 µA.

[0025] Furthermore, regardless of the type of ionized gas and the ampere number, the processing time is preferably at least 1 second and at most 100 minutes, but any processing time within this range is considered useful in the practice of the invention. The processing time can therefore be approximately 2 s, approximately 3 s, approximately 4 s, approximately 5 s, approximately 6 s, approximately 7 s, approximately 8 s, approximately 9 s, approximately 10 s, approximately 11 s, approximately 12 s, approximately 13 s, approximately 14 s, approximately 15 s, approximately 16 s, approximately 17 s, approximately 18 s, approximately 19 s, approximately 20 s, approximately 21 s, approximately 22 s, approximately 23 s, approximately 24 s, approximately 25 s, approximately 26 s, approximately 27 s, approximately 28 s, approximately 29 s, approximately 30 s, approximately 31 s, approximately 32 s, approximately 33 s, approximately 34 s, approximately 35 s, approximately 36 s, approximately 37 s, approximately 38 s, approximately 39 s, approximately 40 s, approximately 41 s, approximately 42 s, approximately 43 s. s, approximately 44 s, approximately 45 s, approximately 46 s, approximately 47 s, approximately 48 s, approximately 49 s, approximately 50 s, approximately 51 s, approximately 52 s, approximately 53 s, approximately 54 s, approximately 55 s, approximately 56 s, approximately 57 s, approximately 58 s, approximately 59 s Approximately 1 minute, approximately 2 minutes, approximately 3 minutes, approximately 4 minutes, approximately 5 minutes, approximately 6 minutes, approximately 7 minutes, approximately 8 minutes, approximately 9 minutes, approximately 10 minutes, approximately 11 minutes, approximately 12 minutes, approximately 13 minutes, approximately 14 minutes, approximately 15 minutes, approximately 16 minutes, approximately 17 minutes, approximately 18 minutes, approximately 19 minutes, approximately 20 minutes, approximately 21 minutes, approximately 22 minutes, approximately 23 minutes, approximately 24 minutes, approximately 25 minutes, approximately 26 minutes, approximately 27 minutes, approximately 28 minutes, approximately 29 minutes, approximately 30 minutes, approximately 31 minutes, approximately 32 minutes, approximately 33 minutes, approximately 34 minutes, approximately 35 minutes, approximately 36 minutes, approximately 37 minutes, approximately 38 minutes, approximately 39 minutes, approximately 40 minutes, approximately 41 minutes, approximately 42 minutes, approximately 43 minutes, approximately 44 minutes, approximately 45 minutes, approximately 46 minutes, approximately 47 minutes, approximately 48 minutes, approximately 49 minutes, approximately 50 minutes. Approximately 51 minutes, approximately 52 minutes, approximately 53 minutes, approximately 54 minutes, approximately 55 minutes, approximately 56 minutes, approximately 57 minutes, approximately 58 minutes, approximately 59 minutes, approximately 60 minutes, approximately 61 minutes, approximately 62 minutes, approximately 63 minutes, approximately 64 minutes, approximately 65 minutes, approximately 66 minutes, approximately 67 minutes, approximately 68 minutes, approximately 69 minutes, approximately 70 minutes, approximately 71 minutes, approximately 72 minutes, approximately 73 minutes, approximately 74 minutes, approximately 75 minutes Approximately 76 minutes, approximately 77 minutes, approximately 78 minutes, approximately 79 minutes, approximately 80 minutes, approximately 81 minutes, approximately 82 minutes, approximately 83 minutes, approximately 84 minutes, approximately 85 minutes, approximately 86 minutes, approximately 87 minutes, approximately 88 minutes, approximately 89 minutes, approximately 90 minutes, approximately 91 minutes, approximately 92 minutes, approximately 93 minutes, approximately 94 minutes, approximately 95 minutes, approximately 96 minutes, approximately 97 minutes, approximately 98 minutes, and approximately 99 minutes.

[0026] In a preferred embodiment, the processing described above is combined with optical processing – for details on useful optical processing modes, see, for example, WO 2015 / 052170.

[0027] Embodiments of the present invention further include a method in the first aspect comprising projecting ionized gas molecules through a gas ion transport device, which may optionally be further equipped with a camera and / or a light-emitting device.

[0028] In embodiments of the present invention, the cosmetic treatment is selected from the group consisting of: wrinkle depth reduction, skin tightening, skin rejuvenation, skin elasticity enhancement, skin hydration enhancement, collagen stimulation, skin peeling, scar repair, dermabrasion, anti-aging, skin discoloration reduction, fine line improvement, redness relief, sensitivity relief, keratolysis, acne reduction, youth glow induction, prevention or repair of sunburn and / or photodamage, anti-inflammatory treatment of the skin, treatment to improve the effect of laser treatment, treatment to improve the effect of cryolipolysis, and reduction of postoperative scars and pigmentation.

[0029] In another preferred embodiment, the cosmetic treatment is selected from the group consisting of: wrinkle depth reduction, skin tightening, skin rejuvenation, skin elasticity enhancement, skin hydration enhancement, collagen stimulation, skin peeling, scar repair, dermabrasion, anti-aging, skin discoloration reduction, fine line improvement, keratolysis, and youth rejuvenation induction.

[0030] In some embodiments, the cosmetic treatment is performed in combination with the topical application of at least one cosmetic active substance or composition. As supported by findings reported in the examples, the delivery of at least one cosmetic active substance or composition is preferably enhanced by the projection of ionized gas molecules and / or the activity of at least one cosmetic active substance is enhanced by the projection of ionized gas molecules. Examples of cosmetic active substances are, for example, vitamins and vitamin precursors, metal salts, moisturizers, lipids, fats or fatty acids (e.g., in liposome form), peptides or polypeptides, and amino acids.

[0031] In these cases, the at least one cosmetic active substance is preferably selected from the group consisting of: beta-carotene and / or other carotenoids, selenium or selenium salts, manganese or manganese salts, one or more antioxidants, glutathione, coenzyme Q10, alpha-lipoic acid, one or more flavonoids, one or more phenolic compounds (such as phenolic compounds selected from quercetin, one or more catechins, resveratrol, coumaric acid, one or more anthocyanins, one or more polyphenols and one or more phytoestrogens), one or more vitamins (e.g. selected from the group consisting of: vitamin A, retinol, B3, vitamin C, vitamin E and vitamin D), iron or iron salts, magnesium or magnesium salts, one or more peptides (including synthetic peptides such as Matrixyl 3000®), one or more proteins, one or more amino acids, one or more essential oils or fats, platelet-rich plasma (PRP), collagen, snail mucin and hyaluronic acid.

[0032] The second aspect of the present invention and its embodiments

[0033] This aspect relates to ionized gas molecules in methods for therapeutic treatment of areas of human skin, methods selected from redness relief, sensitivity relief, acne reduction, prevention or repair of sunburn and / or photodamage, anti-inflammatory treatment of the skin, treatment to improve the effects of laser treatment, treatment to improve the effects of cryolipolysis, and reduction of postoperative scarring and pigmentation. The method includes projecting ionized gas molecules onto an area of ​​skin to establish a current driven by the projection of the ionized gas molecules in or above the area of ​​skin. Optionally, the human subject has at least one discharge electrode attached to a region of the body remote from the surface area of ​​the skin to maintain the current. Therefore, the methods discussed relate to a subset of the treatments detailed in the first aspect of the invention and in the embodiments discussed above. This also means that the features discussed above with respect to the first aspect are applicable to the second aspect with necessary modifications; that is, the method can be performed as described above in the discussion of the methods and embodiments of the first aspect.

[0034] The third aspect of the present invention and its embodiments

[0035] This aspect involves

[0036] a) A method of therapeutic treatment of a surface area of ​​skin for a human subject, the method being carried out by topically applying an active pharmaceutical substance or a composition comprising said substance to the skin, the method comprising projecting ionized gas molecules onto the area of ​​skin to establish a current driven by the projection of the ionized gas molecules in or above the area of ​​skin, wherein the human subject optionally has at least one discharge electrode attached to a region of the body remote from the surface area of ​​the skin to maintain the current, and wherein the projection of the ionized gas molecules enhances the absorption and / or therapeutic effect of the applied active pharmaceutical substance or composition, or

[0037] b) A method for a non-therapeutic cosmetic treatment of a surface area of ​​skin for a human subject, the method being carried out by topically applying a cosmetic active substance or a composition containing said active substance to the skin, the method comprising projecting ionized gas molecules onto the area of ​​skin to establish a current driven by the projection of the ionized gas molecules in or above the area of ​​skin, wherein the human subject optionally has at least one discharge electrode attached to a region of the body remote from the surface area of ​​the skin to maintain the current, and wherein the projection of the ionized gas molecules enhances the absorption and / or cosmetic effect of the applied active cosmetic substance or composition.

[0038] Therefore, both methods utilize the surprising finding in the examples that the projection of ionized gas can enhance the effects of locally applied substances, whether pharmaceutical or cosmetic.

[0039] In this context, the cosmetic substance can be any cosmetic substance discussed above in the context of the first aspect of the invention.

[0040] In relevant circumstances, the active pharmaceutical substance is selected from the following group: antibiotic substances, anti-inflammatory substances, cell-inhibiting or cytotoxic anticancer substances, cytokines, chemokines, hormones, receptor stimulants or activators, receptor antagonists, vasoactive substances, analgesics, and anesthetic substances.

[0041] The exact formulation of an active pharmaceutical ingredient or composition, or a cosmetic active ingredient or composition, can vary. For example, a composition (therapeutic or cosmetic) will typically be selected from the group consisting of: suspensions, oil-in-water emulsions, water-in-oil emulsions, and suspensions. The active pharmaceutical ingredient or composition may be in the form of or introduced into a delivery system selected from creams, foams, gels, lotions, ointments, pastes, powders, shake lotions, solids, sponges, tapes, tinctures, solutions, and transdermal patches. Furthermore, the active ingredient or composition may be in the form of or introduced into a delivery system selected from creams, foams, gels, lotions, ointments, pastes, powders, shake lotions, solids, sponges, tapes, tinctures, solutions, and transdermal patches.

[0042] Example 1

[0043] Human body research

[0044] plan

[0045] A 6-week study was conducted on 10 women aged 32-45. Initially, the entire face was treated for 5 minutes with a cleansing serum (in this case, FUSION MESO's "Deep Cleanser"), followed by a massage of the entire face with a serum mixture containing octapeptides, pentapeptides, and low molecular weight hyaluronic acid (in this case, FUSION F-BTX).

[0046] A microcurrent was applied for 10 min only on the left side of the face using a Wetling W200 device (available from Wetling EU APS in Denmark, among other things). The device was adjusted to emit 1.5 μA in the absence of any light.

[0047] Repeat this procedure, which includes cleansing serum, serum blend, and microcurrent treatment, three more times for a total duration of 40 minutes. At the end of the 40-minute treatment period, thoroughly cleanse your face with a typical cleansing cream (such as FUSION FACE CREAM).

[0048] The same treatment was repeated daily from Monday to Friday for four weeks. Changes in facial wrinkles were assessed using both expert grading and image analysis of digital photographs (using ARAM HUVIS DEVICE and SKIN ANALYZER).

[0049] result

[0050] According to experts, the combination of microcurrent and serum treatment significantly improved the appearance of wrinkles after 6 weeks of treatment, compared to a "serum-only" approach. Skin analysis revealed a 15% reduction in wrinkles on the side of the face treated with ionized gas and serum.

[0051] Example 2

[0052] Treatment plan and results

[0053] The following table lists various preferred processing schemes according to the present invention.

[0054]

[0055] The above treatment methods were evaluated based on their effectiveness, and summarized as follows:

[0056]

[0057]

[0058] Reduced wrinkle depth

[0059] Electrical stimulation, such as that used by Rozhanets AR et al., 2010, Vopr Kurortol Fizioter Lech Fiz Kult. Jul-Aug; (4):26-30. Russian. PMID: 21086592 and As described in [Authors' Name], Int J Environ Res Public Health. 2022 Jul 25;19(15):9055. doi: 10.3390 / ijerph19159055. PMID:35897441, [Information on the potential effects of this treatment on reducing wrinkle depth and rejuvenating aging skin is also supported]. In the study by Rozhanets et al., the combined application of electrical muscle stimulation (EMS) and mesotherapy demonstrated significant improvements in age-related facial changes. EMS may enhance muscle tone and circulation, contributing to a more youthful appearance. Meanwhile, [Information on the potential effects of EMS on wrinkles is also supported]. Other researchers have emphasized the role of microneedling in neck and cleavage rejuvenation, demonstrating its ability to stimulate collagen production and enhance the penetration of skincare products. When combined with electrical stimulation according to the invention, the inventors believe that these techniques target different aspects of skin aging, resulting in overall wrinkle reduction and skin rejuvenation.

[0060] Skin firmness

[0061] Electrical stimulation, as explored in studies such as Chen et al. Biochem Pharmacol. 2023 Dec 9:115979. doi: 10.1016 / j.bcp.2023.115979. e-published before print, PMID: 38081367, Donadio et al. Respir Med. 2022 May;196:106798. doi: 10.1016 / j.rmed.2022.106798. 2022 March 1. PMID:35306386 and Gerasimenko et al. Stomatologiia (Mosk). 2001;80(6):52-5. Russian. PMID: 11881465, is expected to promote skin firmness through various mechanisms. Chen et al. investigated the synergistic effect of methotrexate and electrostimulation in alleviating psoriatic skin inflammation, suggesting that electrostimulation may help reduce skin laxity by suppressing the inflammatory response and potentially enhancing collagen production. In a different context, Donadio et al. examined the effectiveness of electrostimulation in improving muscle strength, but this principle can also be extended to skin tightening, as increased muscle tone can indirectly contribute to a firmer appearance. Furthermore, Gerasimenko et al. discussed the potential of electrostimulation in postoperative care following cheilorhinoplasty, indicating its role in promoting tissue healing and potentially contributing to skin tightening. These findings collectively support the possibility that electrostimulation according to the present invention could be a valuable adjunctive therapy for enhancing skin firmness and overall rejuvenation.

[0062] Skin rejuvenation

[0063] The research conducted by Xu et al. (Acta Mech Sin. 2021;37(12):1843-1871. doi: 10.1007 / s10409-020-01026-2. Published electronically on February 6, 2021, PMID: 33584001; PMCID: PMC7866966) and Chen & Yu (Dermatol Ther. 2003;16(2):87-92. doi: 10.1046 / j.1529-8019.2003.01619.x.PMID: 12919109) elucidates the potential of electrical stimulation in skin rejuvenation. Xu et al.'s research may provide valuable insights into how electrical stimulation affects the skin surface, potentially triggering processes such as increased blood circulation, nerve stimulation, and collagen synthesis, all of which are crucial for skin rejuvenation. Furthermore, Chen & Yu's exploration of acupuncture, electrostimulation, and reflexology in dermatology suggests that electrostimulation may modulate various skin functions, promote cell renewal, and enhance overall skin vitality and appearance. In summary, these findings support the role of electrostimulation according to the invention as a non-invasive method for skin rejuvenation, highlighting its potential for regenerating and renewing the skin.

[0064] Increased skin elasticity

[0065] Xu et al. (Acta Mech Sin. 2021;37(12):1843-1871. doi: 10.1007 / s10409-020-01026-2. Published electronically on February 6, 2021, PMID: 33584001; PMCID: PMC7866966) and Chen & Yu (Dermatol Ther. 2003;16(2):87-92. doi: 10.1046 / j.1529-8019.2003.01619.x.PMID: 12919109) and Tangerino Filho et al. (Tissue Cell. 2016 Jun;48(3):224-34. doi: 10.1016 / j.tice.2016.03.004). Research conducted in this journal (published electronically on March 9, 2016, PMID: 27138327) provides valuable insights into the potential of electrical stimulation to enhance skin elasticity. The study by Xu et al. provides an understanding of how electrical stimulation affects the skin surface, potentially promoting collagen synthesis, improving blood circulation, and stimulating nerve activity—all of which contribute to enhanced skin elasticity. Furthermore, the research on microcurrent therapy by Tangerino Filho et al. demonstrates that electrical stimulation can have a positive impact on tissue repair and regeneration, which can extend to enhancing skin elasticity. Additionally, the discussion of electrical stimulation following cleft lip rhinoplasty by Gerasimenko et al. (see above) suggests its potential role in promoting tissue healing and possibly contributing to enhanced skin elasticity. These findings collectively support the promise of electrical stimulation as a non-invasive method for improving skin elasticity, offering potential benefits for both skin health and appearance.

[0066] Enhanced skin hydration

[0067] The research by Tangerino Filho et al. (2016) also revealed the potential of electrical stimulation, particularly microcurrent therapy, in enhancing skin hydration. Although this study primarily focused on resected elastic cartilage defects in young rats, the principles of microcurrent therapy are thought to extend to enhanced skin hydration. Microcurrent therapy can stimulate cellular activity, promote the production of skin-supporting molecules such as hyaluronic acid, and improve the skin's ability to retain moisture. Furthermore, by promoting tissue repair and regeneration, microcurrent therapy can indirectly contribute to enhanced skin hydration by restoring the skin's barrier function and optimizing its moisture balance. In conclusion, these findings therefore support the potential of electrical stimulation, particularly in the form of microcurrent therapy according to the present invention, as a non-invasive method for improving skin hydration, offering potential benefits for skin health and overall appearance.

[0068] Anti-aging

[0069] The research by Kwon & Moon (Ann Phys Rehabil Med. Nov 2020; 63(6):474-482. doi:10.1016 / j.rehab.2019.09.010. Oct 24, 2019 (e-published). PMID: 31669161) and Zacharias et al. (Int J Exp Pathol. Apr 2023; 104(2):81-95. doi: 10.1111 / iep.12469. Feb 8, 2023 (e-published). PMID: 36752313; PMCID: PMC10009304) provides insights into the potential of electrical stimulation in the anti-aging process. Kwon & Moon's research on the regenerative effects of polydeoxyribonucleotides and microcurrents on rotator cuff healing demonstrates that electrical stimulation can promote tissue repair and regeneration, an important aspect of anti-aging interventions. By stimulating cell activity and enhancing collagen synthesis, electrical stimulation is thought to help restore youthful skin characteristics such as elasticity and firmness. Furthermore, research by Zacarias et al. on the association between arnica montana L. and microcurrents in accelerating dermal remodeling suggests that electrical stimulation may contribute to the repair of skin lesions and the regeneration of aging skin. In summary, these findings support the potential of electrical stimulation according to the present invention as a non-invasive method for combating signs of aging, offering potential benefits for skin health and overall anti-aging interventions.

[0070] Reduced skin discoloration and improved fine lines

[0071] Although the study by Jian et al. (Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. Sep 2003; 20(3):494-6. Chinese. PMID: 14565022) primarily investigated the effects of electrical stimulation on the diurnal rhythm of melatonin in soldiers' plasma, the inventors have recognized that the results indirectly suggest a potential role for electrical stimulation in reducing skin discoloration. Melatonin is known to have antioxidant properties and plays a role in regulating pigmentation in the skin. Therefore, by regulating melatonin levels or diurnal rhythm through electrical stimulation, this technique may indirectly affect skin pigmentation, potentially contributing to reducing skin discoloration and influencing the improvement of fine lines.

[0072] Redness relief

[0073] A study by Lee et al. (Int J Mol Sci. 2022 Feb 24; 23(5):2508. doi: 10.3390 / ijms23052508. PMID: 35269651; PMCID: PMC8910224) demonstrated the potential of microcurrent stimulation (a form of electrical stimulation) to provide relief from redness associated with inflammatory skin conditions. By investigating its effects on the inflammatory response of peptidoglycan-treated macrophages and Propionibacterium acnes-induced skin inflammation, this study revealed that microcurrent stimulation inhibits inflammatory pathways mediated by Toll-like receptor 2 (TLR2) and nuclear factor κB (NF-κB). These pathways are known to play crucial roles in initiating and prolonging inflammatory responses, including redness and irritation. Therefore, by inhibiting TLR2 / NF-κB signaling, microcurrent stimulation has the potential to reduce redness and soothe inflamed skin. Therefore, this study supports the promising role of electrical stimulation according to the invention in alleviating redness associated with inflammatory skin conditions, and provides a non-invasive method for managing skin sensitivity and irritation.

[0074] Sensitivity relief

[0075] Zacarias et al. (Int J Exp Pathol. April 2023; 104(2):81-95. doi: 10.1111 / iep.12469. E-published February 8, 2023, PMID: 36752313; PMCID: PMC10009304) explored the potential of electrical stimulation, particularly microcurrent therapy, in promoting keratolysis and skin remodeling. By examining the effects of microcurrent-associated arnica montana L. on skin lesions, this study showed that electrical stimulation accelerates the process of dermal remodeling, which may involve the shedding of damaged or dead skin cells via keratolysis. Microcurrent therapy is known to stimulate cellular activity and promote tissue regeneration, which can facilitate the removal of dead skin cells and promote the renewal of skin layers. Furthermore, the combination of arnica montana L. with microcurrents may further enhance these effects, potentially enhancing the keratolysis process. In summary, these findings support the potential benefits of electrical stimulation, particularly in the form of microcurrent therapy according to the invention, as a non-invasive method to promote exfoliation and improve skin texture, thus offering potential benefits for skin health and rejuvenation.

[0076] Acne reduction

[0077] Lee H et al. (Int J Mol Sci. 24 Feb 2022; 23(5):2508. doi: 10.3390 / ijms23052508. PMID: 35269651; PMCID: PMC8910224) and Lazarenko NN et al. (VoprKurortol Fizioter Lech Fiz Kult. 2015 Jul-Aug; 92(4):41-47. Russian. doi:10.17116 / kurort2015441-47. PMID: 26595968) both provided scientific evidence for the combined treatment of acne vulgaris with bipolar pulsed current electrical stimulation. Experiments on rabbits demonstrated the effect of bipolar pulsed current on lymph node cell composition, thus generally contributing to a better understanding of certain theoretical aspects of the application of electrical stimulation. Clinical studies using multichannel electrical stimulation and microcurrent therapy have shown that these physical factors may lead to immunogenic remodeling in patients with acne vulgaris, manifested as normalization of peripheral blood cellular composition (leukocytes, lymphocytes, T- and B-lymphocyte counts, and immunoglobulin levels). These findings confirm the effectiveness of the proposed comprehensive treatment, which is responsible for enhancing the overall resistance of patients with acne vulgaris. Furthermore, this treatment approach has been shown to exert an immune-correcting effect and promote the restoration of overall adaptive capacity. As a result, 80.9% of patients with acne vulgaris experienced prolonged disease remission. In addition, the paper by Chen & Yu (Dermatol Ther. 2003;16(2):87-92. doi: 10.1046 / j.1529-8019.2003.01619.x. PMID: 12919109) explores the potential of electrical stimulation as a therapy for acne in a broader dermatological context. Electrical stimulation, along with acupuncture and reflexology, has been explored for its effectiveness in addressing various skin conditions, including acne. While the specific details regarding the role of electrical stimulation in acne treatment are not detailed in the provided citation, it can be inferred that electrical stimulation may modulate skin function and inflammatory processes associated with the pathogenesis of acne. Through its potential to modulate sebum production, reduce inflammation, and promote tissue repair, electrical stimulation may help alleviate acne symptoms and promote clearer skin, thus providing a good basis for the application of electrical stimulation in the treatment of acne as disclosed in this disclosure.

[0078] Prevention or repair of sunburn and / or photodamage

[0079] While the paper by Tsolakidis et al. (Burns. Aug 2022; 48(5):1230-1235. doi:10.1016 / j.burns.2021.09.021. Epub Sep 25, 2021, PMID: 34607727) primarily investigated the effects of wireless microcurrent stimulation on improving blood flow in burn wounds, the inventors have realized that it indirectly demonstrates the potential role of electrical stimulation in addressing sunburn or photodamage. Photodamage caused by prolonged exposure to sunlight often leads to impaired blood flow and tissue healing. Therefore, interventions that improve blood flow, such as microcurrent stimulation, are considered beneficial for the repair and regeneration of sunburned skin. By enhancing circulation, microcurrent stimulation can promote the delivery of oxygen and nutrients to damaged tissues, thereby promoting tissue repair and regeneration. Furthermore, it is believed that the cellular activity involved in the electrostimulation-mediated repair process according to the present invention can potentially accelerate the removal of damaged cells and promote the synthesis of new collagen, which is essential for restoring the health and elasticity of skin damaged by sunburn.

[0080] Anti-inflammatory treatment of the skin

[0081] The paper by Chen et al. (Biochem Pharmacol. Dec 9, 2023: 115979. doi: 10.1016 / j.bcp.2023.115979) explored the synergistic effect of methotrexate and electrostimulation in alleviating recurrent psoriatic skin inflammation by targeting memory T cells. While the primary focus was on the efficacy of combination therapy in controlling psoriasis, the inventors have concluded that it highlights the potential of electrostimulation in modulating inflammatory responses in the skin. Psoriasis is characterized by chronic inflammation driven by a dysregulated immune response, including the activation and proliferation of memory T cells. By inhibiting the activity of memory T cells, electrostimulation may play a role in suppressing the inflammatory cascade associated with psoriasis and other inflammatory skin conditions. This supports the possibility that electrostimulation according to the invention may have broader implications in controlling inflammation in skin diseases beyond psoriasis.

[0082] Treatment to improve laser processing effect

[0083] While the paper by Tsolakidis et al. (Burns. Aug 2022; 48(5):1230-1235. doi:10.1016 / j.burns.2021.09.021. Electronic publication September 25, 2021, PMID: 34607727) primarily investigated the effects of wireless microcurrent stimulation on improving blood flow in burn wounds, the inventors have recognized that it indirectly suggests the potential of electrical stimulation in mitigating laser damage. Laser treatments, particularly ablative and non-ablative procedures, can induce tissue damage and inflammation in the skin. By improving blood flow and promoting tissue repair, microcurrent stimulation may have the potential to accelerate the healing process and mitigate the adverse effects of laser damage. Enhanced blood flow facilitates the delivery of oxygen and nutrients to the affected area, supports tissue regeneration, and reduces inflammation. Therefore, while not directly addressing laser damage, the findings from the study by Tsolakidis et al. support the significance of microcurrent stimulation according to the invention in improving wound healing and tissue recovery, including in cases involving laser-induced damage to the skin.

[0084] Reduce postoperative scarring and pigmentation

[0085] In the study by Gomes et al. (Burns. May 2018, 44(3):636-645. doi: 10.1016 / j.burns.2017.10.006. PMID: 29402577), high-voltage electrical stimulation applied to the donor site of skin grafts showed the potential to accelerate the healing process. While the primary focus was on wound healing, the inventors have concluded that the reported findings indirectly indicate the role of electrical stimulation in postoperative scar management. Postoperative scar formation can result in hypertrophic or keloid scars, which may be accompanied by hyperpigmentation. By accelerating the healing process at the donor site, electrical stimulation could potentially mitigate scar formation and reduce the risk of abnormal scar formation. Furthermore, improved wound healing can contribute to more favorable results in terms of scar appearance, texture, and pigmentation. Therefore, while not directly addressing scar pigmentation, this study supports the potential of electrical stimulation according to the invention to influence scar formation and potentially contribute to more aesthetically pleasing surgical outcomes.

[0086] Hair growth

[0087] In the study by Hwang et al. (Int J Mol Sci. April 22, 2021; 22(9):4361. doi: 10.3390 / ijms22094361. PMID: 33921970; PMCID: PMC8122395), microcurrent stimulation demonstrated its potential role in promoting hair growth in vitro on papillary cells derived from human hair follicles and in animal models. Hair loss / hair loss, whether due to genetic factors, hormonal imbalances, or environmental influences, is a common concern for many individuals. Microcurrent stimulation has emerged as a non-invasive and promising approach to addressing this issue. By applying low levels of current to the scalp, microcurrent stimulation can stimulate hair follicle activity, promote blood circulation, and enhance nutrient delivery to the hair follicles. These effects may potentially prolong the anagen (growth) phase of the hair cycle, leading to thicker hair growth over time. The inventors have found findings from research by Hwang et al. that microcurrent stimulation according to the present invention holds promise as a treatment for hair loss / hair removal. By promoting the proliferation and vitality of papillary cells derived from hair follicles and inducing hair growth in animal models, microcurrent stimulation provides a potential non-pharmacological approach to combat hair loss / hair removal. Furthermore, its non-invasive nature and minimal side effects make it an attractive option for individuals seeking alternative hair restoration methods.

[0088] The ability to enhance drug delivery and / or efficacy

[0089] The inventors have discovered that Luft and Ketterer (J Biomol Screen. Sep 2015; 20(8):932-42. doi: 10.1177 / 1087057115579638. Electronic publication on April 7, 2015, PMID: 25851034; PMCID: PMC4543902), Guillet et al. (Chemphyschem. Oct 6 2017; 18(19):2715-2723. doi: 10.1002 / cphc.201700517. Electronic publication on September 6, 2017, PMID: 28796918), and Gunathilake et al. (Pharm Res. Aug 30 2020; 37(9):178. doi: The paper (10.1007 / s11095-020-02910-z. PMID: 32864721) collectively highlights the potential of electrostimulation in enhancing drug delivery and penetration. Luft and Ketterer (2015) discussed the use of electroporation (a technique involving the application of short electrical pulses to increase cell membrane permeability) for the delivery of siRNA in cells and tissues. By inducing transient pores in the cell membrane, electroporation enables efficient uptake of therapeutic molecules, including drugs and nucleic acids, thereby enhancing their delivery and efficacy. Similarly, Guillet et al. (2017) introduced a needle-free hydrogel / carbon nanotube device for electrostimulated skin drug delivery. This innovative approach utilizes electrostimulation to facilitate drug penetration across the skin barrier, providing a non-invasive and potentially more effective alternative to traditional drug delivery methods. Furthermore, Gunathilake et al. (2020) explored the electrostimulated release of poorly water-soluble drugs from poly(lactic acid) / carboxymethyl cellulose / ZnO nanocomposite membranes. By applying electrical stimulation, drug release from nanocomposite membranes can be controlled and optimized, potentially improving drug bioavailability and therapeutic outcomes. Furthermore, the research by Hwang et al. (Int J Mol Sci. 2021 Apr 22; 22(9):4361. doi: 10.3390 / ijms22094361. PMID: 33921970; PMCID: PMC8122395) further explored the potential applications of electrical stimulation, particularly microcurrent stimulation, in promoting hair growth. While not directly related to drug delivery, this study highlights the broader impact of electrical stimulation on biological processes, including tissue regeneration and cell proliferation. These findings support the potential of microcurrent stimulation according to the invention to stimulate papillary cells derived from hair follicles and promote hair growth, demonstrating its therapeutic potential beyond traditional drug delivery applications.In summary, these studies highlight the versatility of electrical stimulation in biomedical research and its potential as a promising tool for enhancing drug delivery, tissue regeneration, and other therapeutic interventions.

[0090] Implementation plan with numbering

[0091] As will be understood from the foregoing, the present invention particularly relates to embodiments numbered as follows:

[0092] E1. A method for cosmetic treatment of a surface area of ​​skin of a human subject, the method comprising projecting ionized gas molecules onto the area of ​​skin to establish a current driven by the projection of the ionized gas molecules in or above the area of ​​skin, wherein the human subject optionally has at least one discharge electrode attached to a body region remote from the surface area of ​​the skin to maintain the current.

[0093] E2. The method according to the implementation scheme E1, wherein the ionized gas molecules are mainly negatively charged, such as negatively charged oxygen molecules.

[0094] E3. The method according to the implementation scheme E1, wherein the ionized gas molecules are mainly positively charged, such as positively charged nitrogen molecules.

[0095] E4. The method according to any of the embodiments numbered above, wherein the ionized gas molecules originate from the atmosphere.

[0096] E5. The method according to any of the embodiments of the preceding number, wherein the projection of ionized gas molecules establishes an electric current between the surface area of ​​the skin and the underlying tissue.

[0097] E6. The method according to any of the embodiments of the preceding number, wherein the current is at least 0.1 μA.

[0098] E7. The method according to any of the embodiments of the preceding number, wherein the current is at most 100 µA.

[0099] E8. The method according to any of the embodiments of the aforementioned number, wherein the processing time is at least 1 second.

[0100] E9. The method according to any of the aforementioned numbered embodiments, wherein the processing time is at most 100 minutes.

[0101] E10. The method according to any of the embodiments numbered above, wherein the method is combined with light processing.

[0102] E11. The method according to any of the embodiments numbered above, wherein ionized gas molecules are projected by a gas ion transport device.

[0103] E12. The method according to embodiment E11, wherein the device is further equipped with a camera and / or a light-emitting device.

[0104] E13. The method according to any of the embodiments of the preceding number, wherein the cosmetic treatment is selected from the group consisting of:

[0105] Wrinkle depth reduced,

[0106] Firm skin

[0107] Skin rejuvenation

[0108] Increased skin elasticity

[0109] Enhanced skin hydration

[0110] Collagen stimulation,

[0111] Peeling skin

[0112] Scar repair

[0113] Skin dermabrasion,

[0114] Anti-aging

[0115] Reduced skin discoloration

[0116] Improves fine lines

[0117] Redness relieved,

[0118] Sensitivity relief

[0119] Keratin exfoliation

[0120] Acne decreased

[0121] Youthful radiance inducement

[0122] Prevention or repair of sunburn and / or photodamage

[0123] Anti-inflammatory treatment of the skin

[0124] Treatments to improve laser processing effects

[0125] Treatments to improve the effects of cryolipolysis, and

[0126] Reduce postoperative scarring and pigmentation.

[0127] E14. The method according to implementation scheme E13, wherein the cosmetic treatment is selected from the group consisting of:

[0128] Wrinkle depth reduced,

[0129] Firm skin

[0130] Skin rejuvenation

[0131] Increased skin elasticity

[0132] Enhanced skin hydration

[0133] Collagen stimulation,

[0134] Peeling skin

[0135] Scar repair

[0136] Skin dermabrasion,

[0137] Anti-aging

[0138] Reduced skin discoloration

[0139] Improves fine lines

[0140] Keratin peeling, and

[0141] Youthful radiance is the key to rejuvenation.

[0142] E15. The method according to any of the embodiments of the preceding number, wherein the method is performed in combination with topical application of at least one cosmetic active substance or composition.

[0143] E16. The method according to embodiment E15, wherein the delivery of at least one cosmetic active substance or composition is enhanced by the projection of ionized gas molecules and / or the activity of at least one cosmetic active substance is enhanced by the projection of ionized gas molecules.

[0144] E17. The method according to embodiment E15 or E16, wherein at least one cosmetic active substance is selected from the group consisting of:

[0145] β-carotene and / or other carotenoids,

[0146] Selenium or selenium salts

[0147] Manganese or manganese salts

[0148] One or more antioxidants,

[0149] Glutathione,

[0150] Coenzyme Q10,

[0151] Alpha-lipoic acid,

[0152] One or more flavonoids,

[0153] One or more phenolic compounds,

[0154] One or more vitamins,

[0155] Iron or iron salts

[0156] Magnesium or magnesium salts,

[0157] One or more peptides, including synthetic peptides such as Matrixyl 3000.

[0158] One or more proteins,

[0159] One or more amino acids,

[0160] One or more essential oils or fats,

[0161] Platelet-rich plasma (PRP)

[0162] Collagen

[0163] snail mucin,

[0164] Hyaluronic acid, and

[0165] Plant stem cells or other stem cells.

[0166] E18. The method according to embodiment E17, wherein one or more phenolic compounds are selected from the group consisting of: quercetin, one or more catechins, resveratrol, coumaric acid, one or more anthocyanins, one or more polyphenols, and one or more phytoestrogens.

[0167] E19. The method according to implementation scheme E17, wherein one or more vitamins are selected from the group consisting of: vitamin A, retinol, B3, vitamin C, vitamin E and vitamin D.

[0168] E20. Ionized gas molecules in a method for therapeutic treatment of a region of human skin, said method being selected from...

[0169] Redness relieved,

[0170] Sensitivity relief

[0171] Acne decreased

[0172] Prevention or repair of sunburn and / or photodamage

[0173] Anti-inflammatory treatment of the skin

[0174] Treatments to improve laser processing effects

[0175] Treatments to improve the effects of cryolipolysis, and

[0176] Reduce postoperative scarring and pigmentation.

[0177] The method includes projecting ionized gas molecules onto a region of skin to establish a current driven by the projection of the ionized gas molecules in or above the region of skin, wherein the human subject optionally has at least one discharge electrode attached to a body region remote from the surface region of the skin in order to maintain the current.

[0178] E21. Ionized gas molecules for the stated purpose according to embodiment E20, wherein the method is carried out as defined in any one of embodiments E2-E12.

[0179] E22. A method for therapeutic treatment of a surface area of ​​skin in a human subject, the method being carried out by topically applying an active pharmaceutical substance or a composition comprising said substance to the skin, the method comprising projecting ionized gas molecules onto the area of ​​skin to establish a current driven by the projection of the ionized gas molecules in or above the area of ​​skin, wherein the human subject optionally has at least one discharge electrode attached to a region of the body remote from the surface area of ​​the skin to maintain the current, and wherein the projection of the ionized gas molecules enhances the absorption and / or therapeutic effect of the applied active pharmaceutical substance or composition.

[0180] E23. A method for a non-therapeutic cosmetic treatment of a surface area of ​​skin for a human subject, the method being carried out by topically applying a cosmetic active substance or a composition containing said active substance to the skin, the method comprising projecting ionized gas molecules onto the area of ​​skin to establish a current driven by the projection of the ionized gas molecules in or above the area of ​​skin, wherein the human subject optionally has at least one discharge electrode attached to a region of the body remote from the surface area of ​​the skin to maintain the current, and wherein the projection of the ionized gas molecules enhances the absorption and / or cosmetic effect of the applied active cosmetic substance or composition.

[0181] E24. The method according to embodiment E23, wherein the cosmetic active substance is selected from the group described in embodiment E17.

[0182] E25. An active pharmaceutical substance or a composition containing an active pharmaceutical substance, used in a method according to embodiment E22 of the number.

[0183] E26. The method of embodiment E22 of numbered or the active pharmaceutical substance or composition for the said use of embodiment E25 of numbered, wherein the active pharmaceutical substance is selected from the group consisting of: antibiotic substances, anti-inflammatory substances, cell-inhibiting or cytotoxic anticancer substances, cytokines, chemokines, hormones, receptor stimulants or activators, receptor antagonists, vasoactive substances, analgesics and anesthetic substances.

[0184] E27. The method of embodiment E22 of numbered or the active pharmaceutical substance or composition for the said use of embodiment E25 of numbered, wherein the composition is selected from the group consisting of: suspension, oil-in-water emulsion, water-in-oil emulsion and suspension.

[0185] E28. The method of embodiment E22 of numbered claims or the active pharmaceutical ingredient or composition for the said use of any of embodiments E25-27 of numbered claims, wherein the active pharmaceutical ingredient or composition is in the form of or introduced into a delivery system selected from creams, foams, gels, lotions, ointments, pastes, powders, shake emulsions, solids, sponges, tapes, tinctures, solutions and transdermal patches.

Claims

1. Method for the cosmetic treatment of a surface area of the skin of a human subject, said method comprising the projection of ionized gas molecules onto said area of the skin in order to establish an electric current in or above said area of the skin driven by the projection of said ionized gas molecules, wherein said human subject optionally has at least one discharge electrode attached to an area of the body remote from said surface area of the skin in order to maintain said electric current.

2. The method of claim 1, wherein the ionized gas molecules are predominantly negatively charged, such as negatively charged oxygen molecules (O2 - ), or wherein the ionized gas molecules are predominantly positively charged, such as positively charged nitrogen molecules (N2 + ).

3. Method according to any one of the preceding claims, wherein said ionized gas molecules originate from the atmosphere.

4. Method according to any one of the preceding claims, wherein the projection of ionized gas molecules establishes an electric current between the surface area of the skin and the tissue below, such as an electric current of at least 0.1 pA and / or an electric current of at most 100 pA.

5. Method according to any one of the preceding claims, wherein the treatment time is at least 1 second and at most 100 minutes.

6. Method according to any one of the preceding claims, said method being performed in combination with a light treatment.

7. Method according to any one of the preceding claims, wherein said ionized gas molecules are projected by a gas ion transport device, wherein said device is preferably additionally equipped with a camera and / or a light emitting device.

8. Method according to any one of the preceding claims, wherein said cosmetic treatment is selected from the group consisting of: i. reduction of wrinkle depth, ii. skin tightening, iii. skin rejuvenation, iv. skin elasticity enhancement, v. skin hydration enhancement, vi. collagen stimulation, vii. skin peeling, viii. scar repair, ix. dermabrasion, x. anti-aging, xi. reduction of skin discoloration, xii. improvement of fine lines, xiii. reduction of redness, xiv. reduction of sensitivity, xv. keratolysis, xvi. reduction of acne, xvii. induction of a youthful glow, xviii. prevention or repair of sun damage and / or photo damage, xix. anti-inflammatory treatment of the skin, xx. treatment to improve the effect of a laser treatment, xxi. treatment to improve the effect of a cryolipolysis treatment, and xxii. reduction of post-surgical scarring and pigmentation, And wherein, said cosmetic treatment being in particular selected from the group consisting of: I. reduction of wrinkle depth, II. skin tightening, III. skin rejuvenation, IV. skin elasticity enhancement, V. skin hydration enhancement, VI. collagen stimulation, VII. skin peeling, VIII. scar repair, IX. dermabrasion, X. anti-aging, XI. reduction of skin discoloration, XII. improvement of fine lines, XIII. keratolysis, and XIV. induction of a youthful glow.

9. Method according to any one of the preceding claims, said method being performed in combination with the topical application of at least one cosmetic active or composition.

10. Method according to claim 9, wherein the delivery of said at least one cosmetic active or composition is enhanced by the projection of said ionized gas molecules and / or wherein the activity of said at least one cosmetic active is enhanced by the projection of said ionized gas molecules.

11. The method according to claim 9 or 10, wherein the at least one cosmetically active substance is selected from the group consisting of: a. beta carotene and / or other carotenoids, b. selenium or a selenium salt, c. manganese or a manganese salt, d. one or more antioxidants, e. glutathione, f. coenzyme Q10, g. lipoic acid, h. one or more flavonoids, i. one or more phenolic compounds, j. one or more vitamins, k. iron or an iron salt, 1. magnesium or a magnesium salt, m. one or more peptides, including synthetic peptides, such as Matrixyl 3000, n. one or more proteins, o. one or more amino acids, p. one or more essential oils or fats, q. platelet rich plasma (PRP), r. collagen, s. hemolectin, t. hyaluronic acid, and u. plant stem cells or other stem cells.

12. The method according to claim 11, wherein the one or more phenolic compounds are selected from the group consisting of quercetin, one or more catechins, resveratrol, coumaric acid, one or more anthocyanins, one or more polyphenols, and one or more phytoestrogens, or wherein the one or more vitamins are selected from the group consisting of vitamin A, retinol, B3, vitamin C, vitamin E, and vitamin D.

13. Ionized gas molecules for use in a method of therapeutic treatment of a region of human skin, the method being selected from: A. redness mitigation, B. sensitivity mitigation, C. acne reduction, D. sun damage and / or photo damage prevention or repair, E. anti-inflammatory treatment of the skin, F. treatment to improve the effect of laser treatment, G. treatment to improve the effect of cryolipolysis, and H. reduction of post-surgical scarring and pigmentation, wherein the method comprises projecting ionized gas molecules onto the region of skin so as to establish an electrical current in or over the region of skin driven by the projection of the ionized gas molecules, wherein the human subject optionally has at least one discharge electrode attached to a region of the body remote from the surface region of skin so as to maintain the electrical current.

14. Ionized gas molecules for use according to claim 13, wherein the method is carried out as defined in any one of claims 2-12.

15. A method of therapeutic treatment of a surface region of skin of a human subject, the method being carried out by topical application of an active pharmaceutical substance or a composition comprising the substance to the skin, the method comprising projecting ionized gas molecules onto the region of skin so as to establish an electrical current in or over the region of skin driven by the projection of the ionized gas molecules, wherein the human subject optionally has at least one discharge electrode attached to a region of the body remote from the surface region of skin so as to maintain the electrical current, and wherein the projection of the ionized gas molecules enhances the absorption and / or therapeutic effect of the applied active pharmaceutical substance or composition.

16. Method for the non-therapeutic cosmetic treatment of a surface area of the skin of a human subject, by topical application of a cosmetic active substance or of a composition comprising said active substance on said skin, comprising projecting ionized gas molecules onto said area of the skin so as to establish, in or above said area of the skin, an electric current driven by said projection of ionized gas molecules, wherein said human subject optionally has at least one discharge electrode attached to an area of the body remote from said surface area of the skin so as to maintain said electric current, and wherein said projection of ionized gas molecules enhances the absorption and / or the cosmetic effect of the applied active cosmetic substance or composition, wherein said cosmetic active substance is preferably selected from the group described in claim 11.

17. Method according to any one of claims 15-16, wherein said active pharmaceutical substance is selected from the group consisting of antibiotic substances, anti-inflammatory substances, cytostatic or cytotoxic anti-cancer substances, cytokines, chemokines, hormones, receptor stimulators or activators, receptor antagonists, vasoactive substances, analgesics and anesthetic substances, and wherein said composition is preferably selected from the group consisting of suspensions, oil-in-water emulsions, water-in-oil emulsions and suspo-emulsions, and / or wherein said active pharmaceutical or said composition is in the form of or introduced into a delivery system selected from the group consisting of creams, foams, gels, lotions, ointments, pastes, powders, shake emulsions, solids, sponges, tapes, tinctures, solutions and transdermal patches.

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