Grid sample production device for electron microscope

Abstract

The invention relates to the technical field of production of grid samples for electron microscopes, and discloses a grid sample production device for electron microscopes, which comprises a main body assembly and a frame body, a first air cylinder is fixed on the frame body, a tweezers adapter is fixed at the output end of the first air cylinder, and tweezers are arranged below the tweezers adapter. Grids are clamped by the tweezers, and the tweezers adapter is connected with the tweezers through a connecting piece. And the freezing assembly is arranged in the frame body and comprises a freezing piece. The device has the beneficial effects that the connecting piece cooperates with multiple springs through a limiting block and a swing rod, quick disassembly and assembly of tweezers and an adapter are achieved, the direction of the tweezers can be positioned, grid angle deviation is avoided, the operation efficiency and the sample stability are improved, a moving piece, a limiting piece and a screening piece in the freezing assembly are linked, an empty storage barrel is automatically searched, freezing grids are stored in a sealed mode, and the freezing efficiency is improved. A low-temperature environment is guaranteed and storage efficiency and sample quality are improved.

Description

Technical Field

[0001] This invention relates to the field of grid sample production technology for electron microscopes, and in particular to a grid sample production apparatus for electron microscopes. Background Technology

[0002] Electron microscope grid samples are the core carriers for obtaining high-resolution images of biological macromolecules (such as proteins). Their production process requires key steps such as precise loading of protein solution, rapid freezing, and proper storage. In the existing technology, such as the electron microscope grid sample production device with Chinese patent application number 201980058686.0, although it can realize the detection of protein solution volume and rapid freezing of the grid, there are still significant technical defects in the storage of the grid after freezing, which makes it difficult to meet the requirements of efficient and pollution-free production.

[0003] First, existing devices typically use fixed grid storage containers. When transferring frozen grids to these containers, manual identification and adjustment of the empty container are required to align it with the downward path of the tweezers. This process is not only cumbersome and time-consuming, but manual adjustment can also lead to container positioning errors, making it difficult for the tweezers to insert accurately. It may even cause grid damage or protein sample contamination due to collisions with the container edges. Second, the separation of the tweezers from the connecting parts (such as the tweezers adapter) in existing devices relies on manual operation. After placing the grid into the storage container, the connection between the tweezers and the connecting parts must be manually disconnected. During this process, hand contact or ambient airflow can easily contaminate the frozen grid inside the storage container with impurities, affecting the accuracy of subsequent electron microscopy observations. Summary of the Invention

[0004] In view of the problems existing in the above and / or existing grid sample production apparatuses for electron microscopy, the present invention is proposed.

[0005] Therefore, the problem that this invention aims to solve is that existing grid sample production devices require manual positioning when storing grids in empty containers, and the separation of the tweezers and the connecting part relies on manual labor, which easily leads to low production efficiency and grid damage and contamination.

[0006] To solve the above technical problems, the present invention provides the following technical solution: a grid sample production device for an electron microscope, comprising a main body component including a frame, a first cylinder fixed on the frame, a tweezers adapter fixed at the output end of the first cylinder, tweezers disposed below the tweezers adapter, the tweezers holding the grid, and the tweezers adapter and the tweezers being connected by a connector;

[0007] A freezing assembly, disposed within the frame, includes a freezing component. The freezing component includes a support plate fixed within the frame. A cylinder is disposed within the support plate, and a freezing cup and a storage cylinder are fixed within the cylinder.

[0008] In a preferred embodiment of the grid sample production device for electron microscopes according to the present invention, the connecting member includes a connecting seat that slides within the tweezers adapter, the tweezers are fixed to the bottom of the connecting seat, a limit block is fixed to the surface of the connecting seat, a swing arm is rotatably connected within the tweezers adapter, and a locking block is provided at the end of the swing arm.

[0009] In a preferred embodiment of the grid sample production apparatus for electron microscopes described in this invention, the connector further includes a first spring fixed inside the tweezers adapter, and a release block is fixed to one side of the swing arm.

[0010] As a preferred embodiment of the grid sample production device for electron microscopes described in this invention, the connecting member further includes a movable column fixed to one side of the swing arm, a second spring is sleeved on the surface of the movable column, a movable groove is formed on the surface of the tweezers adapter, and the movable column slides in the movable groove.

[0011] As a preferred embodiment of the grid sample production device for electron microscopes described in this invention, the connecting member further includes a limiting strip rotatably connected to the surface of the tweezers adapter, the surface of the moving column is provided with a slot, the limiting strip can be engaged in the slot, a third spring is fixed on one side of the limiting strip, a fixing block is fixed on the other end of the third spring, and the fixing block is fixed to the surface of the tweezers adapter.

[0012] In a preferred embodiment of the grid sample production device for electron microscopes described in this invention, the freezing assembly further includes a moving component, which includes a second cylinder fixed within the frame, a rotating ring rotatably connected to the surface of the cylinder, and the output end of the second cylinder fixed within the rotating ring.

[0013] As a preferred embodiment of the grid sample production device for electron microscopes according to the present invention, the moving part further includes a rotating shaft fixed to the bottom of the cylinder, a rotating wheel fixed to the surface of the rotating shaft, a coil spring fixed to the surface of the rotating wheel, a rotating sleeve rotatably connected to the surface of the rotating wheel, and the other end of the coil spring fixed to the inner wall of the rotating sleeve.

[0014] In a preferred embodiment of the grid sample production apparatus for electron microscopes described in this invention, the moving component further includes a rotating gear fixed to the surface of the rotating sleeve, and a rack is fixed to the bottom of the support disk, wherein the rotating gear and the rack mesh.

[0015] In a preferred embodiment of the grid sample production device for electron microscopes according to the present invention, the freezing assembly further includes a limiting member disposed on the storage cylinder. The limiting member includes a support fixed to the surface of the storage cylinder, a limiting buckle rotatably connected inside the support, a fourth spring fixed inside the limiting buckle, the other end of the fourth spring fixed to the surface of the storage cylinder, a limiting groove formed on the surface of the connecting seat, the end of the limiting buckle inserted into the limiting groove, and a chamfer formed at the end of the limiting buckle.

[0016] In a preferred embodiment of the grid sample production apparatus for electron microscopes according to the present invention, the freezing assembly further includes a screening component disposed on the storage cylinder. The screening component includes a rotating ring rotatably connected to the bottom of the cylinder body. A baffle is fixed to the surface of the rotating ring and slides within the support plate. A sliding sleeve is fixed to the surface of the storage cylinder. A lifting sleeve slides within the sliding sleeve. A support block is fixed to the surface of the lifting sleeve. A fifth spring is fixed to the bottom of the support block. A support plate is fixed to the other end of the fifth spring. The support plate is fixed to the surface of the sliding sleeve. A lifting bar slides within the lifting sleeve. A sixth spring is disposed within the lifting bar. A pressing surface is disposed at the end of the lifting bar. An adjusting plate is rotatably connected within the storage cylinder. A pressing groove is formed within the lifting sleeve, and the end of the adjusting plate is located within the pressing groove.

[0017] The beneficial effects of this invention are as follows: the connector, through the cooperation of the limiting block, the swing rod and multiple springs, enables the quick assembly and disassembly of the tweezers and the adapter, and can also position the direction of the tweezers, avoid grid angle deviation, and improve operating efficiency and sample stability.

[0018] The moving parts, limiting parts, and screening parts in the freezing assembly work together to automatically locate the empty storage cylinder, seal the freezing grid, ensure a low-temperature environment, and improve storage efficiency and sample quality. Attached Figure Description

[0019] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Wherein:

[0020] Figure 1 This is a structural diagram of a grid sample production apparatus used in electron microscopy.

[0021] Figure 2 This is a diagram of the internal structure of a grid sample production device used in electron microscopy.

[0022] Figure 3This is a structural diagram of a tweezers adapter for a grid sample production apparatus used in an electron microscope.

[0023] Figure 4 A grid sample production apparatus for use in electron microscopy Figure 3 Enlarged view of the structure at point A in the middle.

[0024] Figure 5 A grid sample production apparatus for use in electron microscopy Figure 3 Cross-sectional view of the structure at point KK.

[0025] Figure 6 A grid sample production apparatus for use in electron microscopy Figure 5 Enlarged view of the structure at point B in the middle.

[0026] Figure 7 This is a structural diagram of the connector for a grid sample production device used in electron microscopy.

[0027] Figure 8 A grid sample production apparatus for use in electron microscopy Figure 7 Enlarged view of the structure at point C.

[0028] Figure 9 This is a structural diagram of the cylindrical body of a grid sample production apparatus used for electron microscopy.

[0029] Figure 10 A grid sample production apparatus for use in electron microscopy Figure 9 Cross-sectional view of the structure at the LL section.

[0030] Figure 11 A grid sample production apparatus for use in electron microscopy Figure 10 Enlarged view of the structure at point D.

[0031] Figure 12 A grid sample production apparatus for use in electron microscopy Figure 10 Enlarged view of the structure at point E in the middle.

[0032] Figure 13 A grid sample production apparatus for use in electron microscopy Figure 10 Enlarged view of the structure at point F in the middle.

[0033] Figure 14 This is a structural diagram of the support disk of a grid sample production device used in electron microscopy.

[0034] Figure 15 A grid sample production apparatus for use in electron microscopy Figure 14 A magnified view of the structure at point G in the middle.

[0035] Figure 16A grid sample production apparatus for use in electron microscopy Figure 14 Enlarged view of the structure at point H in the middle.

[0036] Figure 17 This is a diagram of the bottom structure of the support disk of a grid sample production device used in electron microscopy.

[0037] In the diagram: Main component 1; Frame 11; First cylinder 12; Tweezers adapter 13; Tweezers 14; Grid 15; Connector 16; Freezing component 2; Freezing component 21; Support plate 211; Cylinder 212; Freezing cup 213; Storage cylinder 214; Quantity 5; Connecting seat 161; Limiting block 162; Swing rod 163; Locking block 163-1; First spring 164; Release block 165; Moving column 166; Second spring 167; Moving groove 13-1; Limiting strip 168; Locking groove 166-1; Third spring 169; Fixing block 1610; Moving component 22; The... 221 Second cylinder; 222 Rotating ring; 223 Rotating shaft; 224 Rotating wheel; 225 Coil spring; 226 Rotating sleeve; 227 Rotating gear; 228 Rack; 23 Limiting component; 231 Support seat; 232 Limiting buckle; 233 Fourth spring; 161-1 Limiting groove; 232-1 Chamfer; 24 Screening component; 241 Rotating ring; 242 Stop bar; 243 Sliding sleeve; 244 Lifting sleeve; 245 Support block; 246 Fifth spring; 247 Support plate; 248 Lifting bar; 249 Sixth spring; 248-1 Extrusion surface; 2410 Adjusting plate; 244-1 Extrusion groove. Detailed Implementation

[0038] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0039] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0040] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that is mutually exclusive with other embodiments.

[0041] Example 1

[0042] Reference Figures 1-3 , Figure 9 , Figure 10 and Figure 14This is the first embodiment of the present invention. This embodiment provides a grid sample production device for an electron microscope. The grid sample production device for an electron microscope includes a main body component 1, including a frame 11. A first cylinder 12 is fixed on the frame 11. A tweezers adapter 13 is fixed at the output end of the first cylinder 12. Tweezers 14 are arranged below the tweezers adapter 13. The tweezers 14 hold a grid 15. The tweezers adapter 13 and the tweezers 14 are connected by a connector 16. The connector 16 can be quickly attached to and detached from the tweezers adapter 13.

[0043] The freezing assembly 2 is disposed within the frame 11 and includes a freezing component 21. The freezing component 21 includes a support plate 211 fixed within the frame 11. A cylinder 212 is disposed within the support plate 211. A freezing cup 213 and a storage cylinder 214 are fixed within the cylinder 212. There are five storage cylinders 214 arranged in a circle within the cylinder 212. The freezing cup 213 is located at the center of the cylinder 212 and contains low-temperature liquid ethane for freezing the sample within the grid 15. A through hole is provided within the frame 11. The output end of the first cylinder 12 moves downward, driving the tweezers adapter 13, connector 16, tweezers 14, and grid 15 through the through hole, thereby allowing the grid 15 to enter the freezing cup 213. After freezing is completed, the output end of the first cylinder 12 resets, which can then move the grid 15 out of the freezing cup 213.

[0044] The space within the frame 11 where the storage cylinder 214 is located is a low-temperature environment, which can prevent the grid 15 stored inside the storage cylinder 214 from thawing.

[0045] Example 2

[0046] Reference Figures 3-8 This is the second embodiment of the present invention, which is based on the previous embodiment.

[0047] Specifically, the connector 16 includes a connector 161 that slides within the tweezers adapter 13. Tweezers 14 are fixed to the bottom of the connector 161. A limiting block 162 is fixed to the surface of the connector 161. The limiting block 162 prevents the connector 161 from rotating relative to the tweezers adapter 13 and positions the tweezers 14, preventing the grid 15 held by the tweezers 14 from shifting at an angle. A swing arm 163 is rotatably connected inside the tweezers adapter 13. A locking block 163-1 is provided at the end of the swing arm 163. When the connector 161 is inserted into the tweezers adapter 13, the limiting block 162 moves upward and pushes the locking block 163-1 away, causing the limiting block 162 to move above the locking block 163-1. This causes the locking block 163-1 to limit the limiting block 162, preventing the limiting block 162 from moving downward and thus preventing the connector 161 from being removed from the tweezers adapter 13, thereby fixing the connector 161.

[0048] Specifically, the connector 16 also includes a first spring 164 fixed inside the tweezers adapter 13. The first spring 164 is currently in a compressed state and is used to push the connector 161 so that the connector 161 stays stably in the current position. A release block 165 is fixed on one side of the swing arm 163 so that the connector 161 continues to move upward, which allows the limiting block 162 to squeeze the release block 165, thereby causing the swing arm 163 to swing and drive the locking block 163-1 at its end away from the connector 161, thereby releasing the limitation on the connector 161 and allowing the connector 161 to be removed from the tweezers adapter 13.

[0049] Specifically, the connector 16 also includes a movable column 166 fixed to one side of the swing arm 163. A second spring 167 is sleeved on the surface of the movable column 166. The second spring 167 is in a compressed state and is used to push the swing arm 163 so that the swing arm 163 is located on one side of the connector 161. A movable groove 13-1 is opened on the surface of the tweezers adapter 13. The movable column 166 slides in the movable groove 13-1. When the swing arm 163 swings, the movable column 166 slides in the movable groove 13-1.

[0050] Specifically, the connector 16 also includes a limiting strip 168 rotatably connected to the surface of the tweezers adapter 13. The surface of the moving column 166 is provided with a slot 166-1, and the limiting strip 168 can be snapped into the slot 166-1. A third spring 169 is fixed on one side of the limiting strip 168, and a fixing block 1610 is fixed on the other end of the third spring 169. The third spring 169 is in a stretched state, and the fixing block 1610 is fixed to the surface of the tweezers adapter 13.

[0051] When the limiting block 162 presses against the release block 165, causing the swing arm 163 to swing and move the locking block 163-1 at its end away from the connecting seat 161, the swing arm 163 continues to compress the second spring 167, while causing the moving column 166 to move outward of the tweezers adapter 13. At this time, the moving column 166 moves the locking groove 166-1 to the side of the limiting strip 168. Under the pulling force of the third spring 169, the limiting strip 168 can be locked into the locking groove 166-1, thereby limiting the moving column 166 and preventing the swing arm 163 from approaching the connecting seat 161 again. At this time, the connecting seat 161 can smoothly slide down and out of the tweezers adapter 13, thereby separating the tweezers adapter 13 and the connecting seat 161.

[0052] Example 3

[0053] Reference Figures 1-17 This is the third embodiment of the present invention, which is based on the first two embodiments.

[0054] Specifically, the refrigeration assembly 2 also includes a movable component 22, which includes a second cylinder 221 fixed inside the frame 11. A rotating ring 222 is rotatably connected to the surface of the cylinder 212. The output end of the second cylinder 221 is fixed inside the rotating ring 222. When the output end of the second cylinder 221 extends, the cylinder 212 can be driven to slide inside the support plate 211 through the rotating ring 222. At this time, the cylinder 212 can rotate arbitrarily inside the rotating ring 222.

[0055] Specifically, the movable component 22 also includes a rotating shaft 223 fixed to the bottom of the cylinder 212. A rotating wheel 224 is fixed to the surface of the rotating shaft 223. A coil spring 225 is fixed to the surface of the rotating wheel 224. A rotating sleeve 226 is rotatably connected to the surface of the rotating wheel 224. The other end of the coil spring 225 is fixed to the inner wall of the rotating sleeve 226.

[0056] By rotating the rotating sleeve 226, the coil spring 225 can be compressed, which in turn drives the rotating wheel 224 to rotate. This causes the rotating wheel 224 to drive the rotating shaft 223 to rotate, which in turn causes the rotating shaft 223 to drive the cylinder 212 to rotate.

[0057] If an external force limits the cylinder 212 at this time, preventing the cylinder 212 from rotating, the coil spring 225 will continue to compress and store energy.

[0058] Specifically, the movable component 22 also includes a rotating gear 227 fixed to the surface of the rotating sleeve 226, and a rack 228 fixed to the bottom of the support disk 211, with the rotating gear 227 and the rack 228 meshing.

[0059] When the output end of the second cylinder 221 extends, the cylinder 212 can drive the rotating gear 227 to move through the rotating shaft 223. Then, through the drive of the rack 228, the rotating gear 227 can rotate, thereby causing the rotating sleeve 226 to rotate, so that the coil spring 225 can be compressed and stored.

[0060] When the output end of the second cylinder 221 retracts, the rack 228 causes the rotating gear 227 to rotate in the opposite direction, which in turn drives the cylinder 212 to rotate in the opposite direction.

[0061] Specifically, the freezing assembly 2 also includes a limiting member 23 disposed on the storage cylinder 214. The limiting member 23 includes a support base 231 fixed to the surface of the storage cylinder 214. A limiting buckle 232 is rotatably connected inside the support base 231. A fourth spring 233 is fixed inside the limiting buckle 232. The fourth spring 233 is in a compressed state. The other end of the fourth spring 233 is fixed to the surface of the storage cylinder 214. Two symmetrical limiting grooves 161-1 are opened on the surface of the connecting seat 161. The end of the limiting buckle 232 is inserted into the limiting groove 161-1 to limit the connecting seat 161, so that the connecting seat 161 is fixed at the opening of the storage cylinder 214. A sealing ring is provided at the bottom of the connecting seat 161 to seal the connection between the connecting seat 161 and the storage cylinder 214. A chamfer 232-1 is provided at the end of the limiting buckle 232.

[0062] When the connecting seat 161 falls from above into the inlet of the storage cylinder 214, it can press the chamfer 232-1 of the two limiting buckles 232, causing the two limiting buckles 232 to move away from each other, so that the connecting seat 161 can fall smoothly into the opening of the storage cylinder 214. At this time, the end of the limiting buckle 232 is inserted into the limiting groove 161-1, thereby limiting the connecting seat 161.

[0063] The output end of the first cylinder 12 continues to extend, causing the limiting block 162 to press against the release block 165, thereby allowing the connecting seat 161 to separate from the tweezers adapter 13. At this time, the output end of the first cylinder 12 retracts, leaving the connecting seat 161 at the opening of the storage cylinder 214. The tweezers 14 and the grid 15 they hold are suspended in the center of the storage cylinder 214, thus allowing for stable storage of the grid 15.

[0064] By pressing the handle of the limit buckle 232, the other end of the limit buckle 232 can be moved away from the limit groove 161-1, thereby releasing the limit on the connecting seat 161, so that the connecting seat 161 can be moved away from the storage cylinder 214, thereby removing the grid 15.

[0065] Specifically, the freezing assembly 2 also includes a screening element 24 disposed on the storage cylinder 214. The screening element 24 includes a rotating ring 241 rotatably connected to the bottom of the cylinder 212. A baffle 242 is fixed on the surface of the rotating ring 241. The baffle 242 slides in the support plate 211. When the output end of the second cylinder 221 pushes the cylinder 212 through the rotating ring 222, the cylinder 212 can drive the rotating ring 241 to move synchronously, and the rotating ring 241 will pull the baffle 242, so that the baffle 242 slides in the support plate 211.

[0066] A sliding sleeve 243 is fixed to the surface of the storage cylinder 214. A lifting sleeve 244 slides inside the sliding sleeve 243. A support block 245 is fixed to the surface of the lifting sleeve 244. A fifth spring 246 is fixed to the bottom of the support block 245. A support plate 247 is fixed to the other end of the fifth spring 246. The fifth spring 246 is in a stretched state. The support plate 247 is fixed to the surface of the sliding sleeve 243. The lifting sleeve 244 can be moved downward by the tension of the fifth spring 246. A lifting bar 248 slides inside the lifting sleeve 244. A sixth spring 249 is provided inside the lifting bar 248. The sixth spring 249 is in a compressed state. An extrusion surface 248-1 is provided at the end of the lifting bar 248. An adjusting plate 2410 is rotatably connected inside the storage cylinder 214. An extrusion groove 244-1 is opened inside the lifting sleeve 244. The end of the adjusting plate 2410 is located in the extrusion groove 244-1.

[0067] In the state shown in the figure, one end of the adjusting plate 2410 is pressed down by the connecting seat 161, causing the other end of the adjusting plate 2410 to rise. By adjusting the plate 2410 to move the extrusion groove 244-1, the lifting sleeve 244 can be moved upward, thereby driving the lifting bar 248 to move upward, so that the end of the lifting bar 248 retracts into the cylinder 212.

[0068] The adjusting plate 2410 on the other storage cylinders 214 without grid 15 is not squeezed, and the end of the lifting bar 248 is located below the cylinder 212. At this time, when the second cylinder 221 pushes the cylinder 212, it can drive the cylinder 212 to rotate. When the storage cylinder 214 without grid 15 moves above the stop bar 242, the end of the lifting bar 248 will be engaged with one side of the stop bar 242, thereby stopping the cylinder 212 from rotating. At this time, the cylinder 212 moves horizontally as a whole until the storage cylinder 214 moves below the output end of the first cylinder 12.

[0069] When the grid 15 is frozen, the output end of the first cylinder 12 moves downward, driving the tweezers adapter 13, connector 16, tweezers 14 and grid 15 through the through hole in the frame 11, so that the grid 15 enters the freezing cup 213. After freezing is completed, the output end of the first cylinder 12 is reset, which can drive the grid 15 out of the freezing cup 213.

[0070] When the grid 15 needs to be stored, the second cylinder 221 first pushes the cylinder 212, which causes the cylinder 212 to move through the rotating shaft 223 and drive the rotating gear 227. Then, through the drive of the rack 228, the rotating gear 227 rotates, which in turn drives the rotating sleeve 226 to rotate. This causes the coil spring 225 to compress and store energy, thereby driving the cylinder 212 to rotate. As the cylinder 212 rotates, when the storage cylinder 214 without the grid 15 moves above the stop bar 242, the end of the lifting bar 248 will engage with one side of the stop bar 242, thus stopping the cylinder 212 from rotating. At this time, the cylinder 212 moves horizontally until the storage cylinder 214 moves below the output end of the first cylinder 12.

[0071] At this time, the output end of the first cylinder 12 moves downward, and the connecting seat 161 falls from above to the inlet of the storage cylinder 214, which can squeeze the chamfer 232-1 of the two limit buckles 232, so that the two limit buckles 232 move away from each other, and the connecting seat 161 falls smoothly at the opening of the storage cylinder 214. At this time, the end of the limit buckle 232 is inserted into the limit groove 161-1, thereby limiting the connecting seat 161.

[0072] The output end of the first cylinder 12 continues to extend, causing the limiting block 162 to press against the release block 165, thereby allowing the connecting seat 161 to separate from the tweezers adapter 13. At this time, the output end of the first cylinder 12 retracts, leaving the connecting seat 161 at the opening of the storage cylinder 214. The tweezers 14 and the grid 15 they hold are suspended in the center of the storage cylinder 214, thus allowing for stable storage of the grid 15.

[0073] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A grid sample production apparatus for an electron microscope, characterized by: include, The main component (1) includes a frame (11), on which a first cylinder (12) is fixed. A tweezers adapter (13) is fixed at the output end of the first cylinder (12). Tweezers (14) are provided below the tweezers adapter (13). The tweezers (14) hold a grid (15). The tweezers adapter (13) and the tweezers (14) are connected by a connector (16). The freezing component (2) is disposed within the frame (11) and includes a freezing element (21). The freezing element (21) includes a support plate (211) fixed within the frame (11). A cylinder (212) is disposed within the support plate (211). A freezing cup (213) and a storage cylinder (214) are fixed within the cylinder (212).

2. The grid sample production apparatus for an electron microscope according to claim 1, characterized by: The connector (16) includes a connector (161) that slides within the tweezers adapter (13), the tweezers (14) being fixed to the bottom of the connector (161), a limit block (162) being fixed to the surface of the connector (161), a swing arm (163) being rotatably connected within the tweezers adapter (13), and a locking block (163-1) being provided at the end of the swing arm (163).

3. The grid sample production apparatus for an electron microscope as claimed in claim 2, characterized by: The connector (16) also includes a first spring (164) fixed inside the tweezers adapter (13), and a release block (165) is fixed on one side of the swing arm (163).

4. The grid sample production apparatus for an electron microscope as claimed in claim 3, characterized by: The connector (16) also includes a movable column (166) fixed to one side of the swing rod (163). A second spring (167) is sleeved on the surface of the movable column (166). A movable groove (13-1) is opened on the surface of the tweezers adapter (13). The movable column (166) slides in the movable groove (13-1).

5. The grid sample production apparatus for an electron microscope as claimed in claim 4, characterized by: The connector (16) also includes a limiting strip (168) rotatably connected to the surface of the tweezers adapter (13). The surface of the moving column (166) is provided with a slot (166-1). The limiting strip (168) can be snapped into the slot (166-1). A third spring (169) is fixed on one side of the limiting strip (168). A fixing block (1610) is fixed on the other end of the third spring (169). The fixing block (1610) is fixed to the surface of the tweezers adapter (13).

6. The grid sample production apparatus for an electron microscope as claimed in claim 5, characterized by: The refrigeration assembly (2) also includes a movable component (22), which includes a second cylinder (221) fixed inside the frame (11). A rotating ring (222) is rotatably connected to the surface of the cylinder (212), and the output end of the second cylinder (221) is fixed inside the rotating ring (222).

7. The grid sample production apparatus for an electron microscope as claimed in claim 6, characterized by: The movable component (22) also includes a rotating shaft (223) fixed to the bottom of the cylinder (212). A rotating wheel (224) is fixed on the surface of the rotating shaft (223). A coil spring (225) is fixed on the surface of the rotating wheel (224). A rotating sleeve (226) is rotatably connected to the surface of the rotating wheel (224). The other end of the coil spring (225) is fixed to the inner wall of the rotating sleeve (226).

8. The grid sample production apparatus for an electron microscope as claimed in claim 7, characterized by: The moving piece (22) further comprises a rotating gear (227) fixed to the surface of the rotating sleeve (226), and a rack (228) is fixed to the bottom of the support disc (211), and the rotating gear (227) and the rack (228) are engaged.

9. The grid sample production apparatus for an electron microscope as claimed in claim 8, characterized by: The freezing assembly (2) further comprises a limiting piece (23) arranged on the storage cylinder (214), the limiting piece (23) comprises a support base (231) fixed to the surface of the storage cylinder (214), a limiting buckle (232) is rotatably connected in the support base (231), a fourth spring (233) is fixed in the limiting buckle (232), the other end of the fourth spring (233) is fixed to the surface of the storage cylinder (214), a limiting groove (161-1) is arranged on the surface of the connecting seat (161), the end of the limiting buckle (232) is inserted into the limiting groove (161-1), and a chamfer (232-1) is arranged on the end of the limiting buckle (232).

10. The grid sample production apparatus for an electron microscope according to claim 1 or 9, characterized by: The freezing assembly (2) further comprises a screening piece (24) arranged on the storage cylinder (214), the screening piece (24) comprises a rotating ring (241) rotatably connected to the bottom of the cylinder body (212), a blocking strip (242) is fixed to the surface of the rotating ring (241), the blocking strip (242) slides in the support disc (211), a sliding sleeve (243) is fixed to the surface of the storage cylinder (214), a lifting sleeve (244) slides in the sliding sleeve (243), a support block (245) is fixed to the surface of the lifting sleeve (244), a fifth spring (246) is fixed to the bottom of the support block (245), a support plate (247) is fixed to the other end of the fifth spring (246), the support plate (247) is fixed to the surface of the sliding sleeve (243), a lifting strip (248) slides in the lifting sleeve (244), a sixth spring (249) is arranged in the lifting strip (248), an extrusion surface (248-1) is arranged on the end of the lifting strip (248), an adjusting plate (2410) is rotatably connected in the storage cylinder (214), an extrusion groove (244-1) is arranged in the lifting sleeve (244), and the end of the adjusting plate (2410) is located in the extrusion groove (244-1).

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