Stem cell culture dish for medical beauty experiment
By designing docking devices, isolators, and sealing mechanisms, the problem of wear on the sealing strips of existing multi-chamber culture dishes has been solved, enabling efficient, sterile, and independent culture of stem cells in culture dishes, which is suitable for multivariate comparative studies in medical aesthetic experiments.
Patent Information
- Application Number
- CN202511493739.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-20
- Publication Date
- 2026-01-16
AI Technical Summary
In existing multi-chamber stem cell culture dishes, the sealing strip is prone to wear during the spiral connection between the top cover and the base, resulting in a decrease in sealing performance and failing to meet the high sterility and high independence requirements of medical aesthetic experiments.
The structure is designed with docking devices, isolators, sealing mechanisms and unlocking devices. It achieves precise guidance through the sliding cooperation of docking plates and guide plates, and the positioning teeth are fixed by the engagement of positioning grooves. The double sealing of sealing strips and expansion airbags avoids the rotation and friction of the top cover, ensuring the independence and sterility of each chamber.
It extends the lifespan of sealing components, reduces operation time, ensures the accuracy of experimental data, avoids cross-contamination, adapts to the needs of multivariate comparative studies, and meets the requirements of efficient parallel experiments in medical aesthetics.
Smart Images
Figure CN121343733A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of culture dishes, in particular to a stem cell culture dish for medical cosmetic experiments. BACKGROUND
[0002] In the field of medical cosmetics, stem cell research is the core direction to realize skin repair, anti-aging and regeneration technology breakthrough. Stem cells are widely used in cell activity detection, skin care product efficacy evaluation, skin tissue regeneration simulation and other scenes in cosmetic experiments due to their strong proliferation and differentiation capacity. The structure design of the culture dish as a key carrier for in vitro culture of stem cells directly affects the stability, sterility and accuracy of the experimental data of the culture environment. With the development of medical cosmetic experiments towards "multi-variable comparison, high efficiency and parallel", single-chamber culture dishes have been difficult to meet the needs of simultaneous research on the growth status of stem cells under different culture medium formulations and different culture conditions. Therefore, multi-chamber stem cell culture dishes have become the standard configuration for experiments, and higher requirements for their sealing performance and structural stability have been put forward.
[0003] The existing stem cell culture dishes for medical cosmetic experiments have undergone upgrading from single-chamber to multi-chamber. Early single-chamber culture dishes can only be used for stem cell culture under a single condition, and the experimental efficiency is relatively low. To adapt to the needs of multi-group comparison experiments, multi-chamber culture dishes have appeared on the market, which are mainly composed of a base, a top cover and a sealing element. The base is made of plastic or glass and is divided into 2-6 independent culture chambers by a partition. Each chamber can be added with different culture media and seeded with stem cells from different sources. The top cover is connected to the base by a screw connection, and the opening and closing are achieved by rotating the top cover. To ensure the sterile environment of each chamber and prevent evaporation of the culture medium, a sealing strip made of rubber or silicone is embedded in the inner wall of the top cover or the top of the base partition. When the top cover is tightened, the sealing strip tightly adheres to the surface of the base, forming a sealed space to ensure the independent operation of each chamber.
[0004] The existing multi-chamber culture dishes have significant defects in actual use. The top cover and the base are connected by a screw connection, and during the rotation process of installing the top cover to the base, the sealing strip in the inner wall of the top cover continuously rubs against the top of the base partition or the edge of the chamber. Long-term use can cause the sealing strip to wear and deform, resulting in a decrease in sealing performance and the destruction of the independence of each chamber, which increases the risk of cross-contamination and makes it difficult to meet the core needs of medical cosmetic experiments for stem cell culture with high sterility and high independence. SUMMARY
[0005] The present application aims to provide a stem cell culture dish for medical cosmetic experiments to solve the problems raised in the background art.
[0006] To achieve the above object, the present application provides the following technical scheme: a medical cosmetology experiment stem cell culture dish, comprising a culture dish body composed of a base and a cover, the culture dish body is provided with a docking device, an isolator, a sealing mechanism, an unlocker and a mounting mechanism, the docking device is used for fixing the connection between the base and the cover, the sealing mechanism is used for sealing the connection between the isolator and the culture dish body and the connection between the base and the cover, the unlocker is used for releasing the restriction of the docking device on the culture dish body, and the mounting mechanism is used for mounting the isolator into the culture dish body; The isolator is used for dividing the culture dish body into multiple culture chambers, and the isolator is composed of an isolation barrel connected with the base and an isolation plate. The docking device comprises: a docking box mounted at the bottom of the cover and a positioning block rotatably mounted in the isolation barrel, and the outer wall of the docking box is provided with a first connecting groove corresponding to the positioning block; A plurality of positioning teeth are mounted on the inner wall of the first connecting groove, the outer wall of the positioning block is provided with a plurality of positioning grooves, the top surfaces of the plurality of positioning grooves are in contact with the top surfaces of the corresponding positioning teeth, the top surfaces of the positioning teeth and the top surfaces of the positioning grooves are flat, and the outer wall of the positioning teeth close to the positioning grooves is designed as an inclined surface. The unlocker comprises a transmission disc rotatably mounted at the bottom of the docking box and a transmission block mounted on the outer wall of the transmission disc, the transmission block pushes the positioning block outward during rotation, and the top of the transmission disc is connected with a torsion block located at the top of the cover and rotatably connected with the cover.
[0007] Preferably, the culture dish body further comprises: a docking groove symmetrically provided on the outer wall of the base, and the docking groove is slidably connected with a docking plate integrally mounted on the outer wall of the cover; a guide plate integrally mounted on the outer wall of the base, the outer wall of the docking plate is provided with a guide groove corresponding to the guide plate, and the bottom of the guide groove is connected with a receiving groove provided on the outer wall of the cover.
[0008] Preferably, the outer ends of the docking plate and the guide plate protrude from the cover and the base, the thickness of the top end of the guide plate is less than the thickness of the bottom end, and the inner wall of the guide plate is in contact with the inner wall of the guide groove. The top surface of the base is in contact with the bottom surface of the cover, and the position where the cover and the base are in contact is provided with a sealing gasket.
[0009] Preferably, a plurality of first connecting grooves are symmetrically mounted on the outer wall of the docking box, and the width of the first connecting groove is greater than the width of the positioning block. A plurality of positioning blocks are isometrically mounted in the middle of the isolation barrel, the inner walls of the plurality of positioning blocks are in contact with the inner walls of the first connecting grooves, and the side edges of the top of the positioning block close to the docking box are designed as arc corners.
[0010] Preferably, the sealing mechanism comprises a sealing strip and an inflatable air bag installed at the corners of the outer wall of the isolation barrel and the isolation plate, and the sealing strip protrudes from the isolation barrel and the isolation plate, and after the base is connected with the top cover, the sealing strip will be attached to the inner wall of the base and the inner wall of the top cover. The inflatable air bag is arranged on the outer wall of the isolation plate, and the cross section of the inflatable air bag is designed in the shape of a Chinese character “fang”, and the outer end of the inflatable air bag does not protrude from the outer wall of the isolation plate when it is not inflated.
[0011] Preferably, the isolator is provided with a gas supply mechanism corresponding to the inflatable air bag, which comprises: An inflation groove is arranged in the inner wall of the isolation barrel, and a piston ring is slidably installed in the inflation groove, and the top of the piston ring is connected with a lower pressing ring which is slidably connected with the isolation barrel, and the top of the lower pressing ring is located at the top of the isolation barrel, and during the connection of the top cover and the base, the top surface of the lower pressing ring will be attached to the bottom surface of the top cover. A reset spring is installed in the inflation groove, and the top of the reset spring is connected with the bottom surface of the inflation groove. A gas guide groove is arranged in the inner part of the isolation plate, and the gas guide groove is in communication with the inflation groove. An air bag groove is arranged on the outer wall of the gas guide groove, and the opening of the air bag groove is covered and sealed by the inflatable air bag. A plurality of air holes are arranged in the inner part of the isolation plate, and the two ends of the air holes are in communication with the gas guide groove and the air bag groove.
[0012] Preferably, the width of the cross section of the inflation groove is greater than the diameter of the gas guide groove, and the diameter of the gas guide groove is greater than the diameter of the air hole.
[0013] Preferably, the unlocker further comprises: A transmission column is rotatably installed in the docking box and rotatably connected with the top cover, and the bottom end of the transmission column is fixedly connected with the transmission disc, and the top end is fixedly connected with the torsion block. A clock spring is installed in the inner part of the docking box and fixedly connected with the outer wall of the transmission column, for resetting the rotated transmission column. A pull ring is installed on the top of the torsion block.
[0014] Preferably, the diameter of the transmission disc is smaller than the distance between the inner walls of the positioning block, and a plurality of transmission blocks are installed at equal angles on the outer wall of the transmission disc, one side of the outer wall of the transmission block is designed as a plane and attached to the corner of the positioning block, and the other side of the outer wall is designed as an inclined surface. The distance between the outer walls of the two transmission blocks which are symmetrical to each other is greater than the distance between the inner walls of the positioning block, and when the transmission blocks start to rotate with the transmission disc, the inclined surface of the transmission block will guide it to rotate to the state of being attached to the inner wall of the positioning block.
[0015] Preferably, the mounting mechanism comprises: The installation groove is arranged in the inner wall of the bottom of the base, and the installation groove is in sliding connection with the bottom of the isolation barrel and is in close contact with the sealing strip on the outer wall of the isolation barrel at the top opening of the installation groove; The rectangular block is integrally installed at the bottom of the isolation barrel, the inner wall of the bottom of the base is provided with a rectangular groove corresponding to the rectangular block, and the rectangular groove is communicated with the installation groove; The connecting column is installed in the rectangular groove, and the bottom of the rectangular block is provided with a second connecting groove corresponding to the connecting column; The bolt is slidably installed at the bottom of the isolation barrel, and the top of the connecting column is provided with a threaded hole in threaded connection with the bolt.
[0016] Compared with the prior art, the medical cosmetology experiment stem cell culture dish has the following beneficial effects: 1. The medical cosmetology experiment stem cell culture dish, by setting the docking plate, guide plate and other parts, the top cover and the base are precisely guided through the sliding cooperation of the docking plate and the guide plate, the positioning block of the docking device is quickly clamped through the inclined surface and the arc-shaped fillet design of the docking box, the positioning teeth are in close contact with the positioning groove plane to enhance the fixing stability, the top cover does not need to be rotated throughout the process, the friction and wear of the sealing strip caused by traditional screw connection are avoided, and the service life of the sealing part is prolonged; when unlocking, only the torsion block needs to be rotated, and the transmission block can push the positioning block to release the fixation, so that the operation is convenient and efficient, and the experiment preparation and operation time is reduced; 2. The medical cosmetology experiment stem cell culture dish, by setting the isolator, the isolator divides the culture dish body into multiple independent culture chambers through the isolation barrel and the isolation plate, and can simultaneously carry out stem cell culture experiments under different culture medium formulas and different culture conditions, so as to meet the needs of multivariate comparison research in medical cosmetology experiments; the installation mechanism cooperates with the installation groove, the rectangular block and the connecting column to precisely fix the isolator and the base, and the sealing strip is in advance in close contact with the inner wall of the base, so as to ensure the initial sealing property of each chamber, avoid cross contamination in the experiment process, and ensure the accuracy of the experimental data; 3. The medical cosmetology experiment stem cell culture dish, by setting the sealing mechanism, the sealing strip of the sealing mechanism protrudes from the outer wall of the isolation barrel and the isolation plate, and after the top cover is docked with the base, it is in close contact with the inner wall of the culture dish body, forming a first physical sealing barrier; during the pressing process of the top cover, the lower pressing ring of the gas supply mechanism is extruded, the piston ring is pushed to press the gas into the inflatable air bag through the gas guide groove and the air hole, so that the inflatable air bag is further in close contact with the inner wall to form a second sealing, and double protection effectively blocks the entry of external pollutants and the leakage of the internal culture environment, which meets the strict requirements of stem cell culture on sterility; 4、The medical beauty experiment uses stem cell culture dish, through setting up docking device, multiple groups of positioning block in docking device are distributed at equal angle, ensure that the top cover and the base are uniformly stressed, fixed firmly; the clock spring of the unlocking device can automatically reset the transmission part, without manual adjustment; the pull ring design is convenient for the top cover to take and place, the guide plate and the storage groove cooperate to assist the stable lifting of the top cover; the installation mechanism realizes the detachable fixing of the isolator through bolts and threaded holes, which is convenient for cleaning and maintenance or replacement of different specifications of isolators, and adapts to diversified experimental scenes. BRIEF DESCRIPTION OF DRAWINGS
[0017] Figure 1 The structure schematic diagram of the application in use; Figure 2 The structure schematic diagram of the top cover and docking device and other parts of the application; Figure 3 The structure schematic diagram of the base and docking device and other parts of the application; Figure 4 The structure schematic diagram of the application in use after being cut open; Figure 5 The structure schematic diagram of the application after disassembling the parts; Figure 6 The structure schematic diagram of the docking device and the unlocking device when connected; Figure 7 The structure schematic diagram of the docking device and the unlocking device after disassembling the parts; Figure 8 The structure schematic diagram of the docking device and the unlocking device when the unlocking device is started; Figure 9 The structure schematic diagram of the docking device and the unlocking device when the unlocking device is started; Figure 10 The structure schematic diagram of the isolator from another perspective; Figure 11 The structure schematic diagram of the isolator after being cut open; Figure 12 The structure schematic diagram of the application Figure 11 The structure schematic diagram of the application after taking out the piston ring, the pressing ring and the reset spring from the inflation groove; Figure 13 The structure schematic diagram of the isolator barrel after taking down the inflatable air bag after being cut open.
[0018] In the figure: 1, culture dish body; 10, base; 100, docking groove; 101, guide plate; 11, top cover; 110, docking plate; 111, guide groove; 112, storage groove; 2, docking device; 20, docking box; 21, positioning block; 22, first connecting groove; 23, positioning tooth; 24, positioning groove; 3, isolator; 30, isolation barrel; 31, isolation plate; 4, sealing mechanism; 40, sealing strip; 41, inflatable air bag; 5, air supply mechanism; 50, inflation groove; 51, piston ring; 52, pressing ring; 520, return spring; 53, air guide groove; 54, air bag groove; 55, air hole; 6, unlocker; 60, transmission column; 601, clock spring; 61, transmission disc; 62, transmission block; 63, torsion block; 64, pull ring; 7, mounting mechanism; 70, mounting groove; 71, rectangular block; 72, rectangular groove; 73, connecting column; 74, second connecting groove; 75, bolt; 76, threaded hole. DETAILED DESCRIPTION
[0019] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.
[0020] Please refer to Figures 1 to 13 The present application provides a technical solution: a stem cell culture dish for medical beauty experiments, which comprises a culture dish body 1, a docking device 2, an isolator 3, a sealing mechanism 4, an air supply mechanism 5, an unlocker 6, and a mounting mechanism 7, and other components. The culture dish body 1 is a basic carrier for stem cell culture and is composed of a base 10 and a top cover 11. The base 10 has a docking groove 100 symmetrically formed on the outer wall, and the top cover 11 has a docking plate 110 integrally installed on the outer wall. The docking plate 110 is in sliding connection with the docking groove 100, thereby achieving the preliminary guidance of the top cover 11 and the base 10. The base 10 has a guide plate 101 integrally installed on the outer wall, and the docking plate 110 has a guide groove 111 formed on the outer wall. The guide groove 111 has a receiving groove 112 connected at the bottom. The guide plate 101 has a top end thickness smaller than a bottom end thickness, which can guide the precise docking of the top cover 11 and sliding into the receiving groove 112. The top surface of the base 10 and the bottom surface of the top cover 11 are attached, and the attachment part is provided with a sealing gasket, which preliminarily blocks the external pollution. The outer ends of the docking plate 110 and the guide plate 101 protrude from the top cover 11 and the base 10, which facilitates the docking operation.
[0021] Specifically, the docking device 2 in the embodiment is used to fix the connection of the base 10 and the top cover 11, and specifically comprises a docking box 20, a positioning block 21, a first connecting groove 22, a positioning tooth 23, and a positioning groove 24. Wherein, the docking box 20 is installed at the bottom of the top cover 11, the positioning block 21 is rotatably installed inside the isolation barrel 30, the outer wall of the docking box 20 is provided with a first connecting groove 22 corresponding to the positioning block 21, the width of the first connecting groove 22 is greater than the width of the positioning block 21, so that the positioning block 21 is easily clamped in; Secondly, a plurality of positioning teeth 23 are installed on the inner wall of the first connecting groove 22, a plurality of groups of positioning grooves 24 are formed on the outer wall of the positioning block 21, the top surfaces of the positioning teeth 23 and the positioning grooves 24 are flat and abutted, so that the fixing stability is enhanced; the outer wall of the positioning teeth 23 close to the positioning grooves 24 is designed as an inclined surface, and the corner close to the docking box 20 on the top of the positioning block 21 is designed as an arc corner, so that the mutual extrusion and guidance during docking are facilitated; a plurality of groups of the positioning block 21 are installed at equal angles in the middle of the isolation barrel 30, and the inner wall thereof is abutted with the inner wall of the first connecting groove 22, so that the uniform fixing is ensured.
[0022] The isolation device 3 in the embodiment is used for dividing the culture dish body 1 into a plurality of culture chambers, which is composed of the isolation barrel 30 and the isolation plate 31, wherein the isolation barrel 30 is connected with the base 10, and a plurality of groups of the isolation plate 31 are installed at equal angles on the outer wall of the isolation barrel 30, and the plurality of groups of the isolation plate 31 divide independent chambers in cooperation with the isolation barrel 30.
[0023] For example, the sealing mechanism 4 is used for sealing the connection between the isolation device 3 and the culture dish body 1, the base 10 and the top cover 11, which specifically includes a sealing strip 40 and an inflatable air bag 41. Wherein, the sealing strip 40 is installed on the corner of the outer wall of the isolation barrel 30 and the isolation plate 31 and protrudes from the surface of the isolation barrel 30 and the isolation plate 31, after the connection between the base 10 and the top cover 11, the sealing strip 40 is abutted with the inner wall of the base 10 and the inner wall of the top cover 11, to form the first sealing. Secondly, the inflatable air bag 41 is installed on the outer wall of the isolation plate 31, and the cross section thereof is designed as a “” character, the outer end thereof does not protrude from the outer wall of the isolation plate 31 when not inflated, and the sealing effect can be enhanced after inflation.
[0024] In the embodiment, the isolation device 3 is provided with a gas supply mechanism 5 corresponding to the inflatable air bag 41, and the gas supply mechanism 5 specifically includes an inflation groove 50, a piston ring 51, a pressing ring 52, a return spring 520, a gas guide groove 53, an air bag groove 54 and an air hole 55. Wherein, the inflation groove 50 is formed on the inner wall of the isolation barrel 30, the piston ring 51 is slidably installed in the inflation groove 50, the pressing ring 52 is connected with the top of the piston ring 51, and the top end of the pressing ring 52 is located at the top of the isolation barrel 30, and the pressing ring 52 is pressed when the top cover 11 is connected with the base 10; Secondly, the reset spring 520 is installed in the inflation groove 50 for resetting the piston ring 51; the gas guide groove 53 is arranged in the inner wall of the isolation plate 31 and communicates with the inflation groove 50, the airbag groove 54 is arranged in the outer wall of the gas guide groove 53 and is sealed and blocked by the inflatable airbag 41, and the air holes 55 are arranged in multiple groups in the inner wall of the isolation plate 31 and communicate with the gas guide groove 53 and the airbag groove 54 at both ends; At the same time, the cross-sectional width of the inflation groove 50 is greater than the diameter of the gas guide groove 53, and the diameter of the gas guide groove 53 is greater than the diameter of the air hole 55, so as to ensure smooth gas delivery.
[0025] For example, the unlocker 6 is used to release the restriction of the adapter 2, which specifically includes a transmission column 60, a clock spring 601, a transmission disc 61, a transmission block 62, a torsion block 63 and a pull ring 64. The transmission column 60 is rotatably installed in the docking box 20 and rotatably connected with the top cover 11, the bottom end of the transmission column 60 is fixedly connected with the transmission disc 61, and the top end is fixedly connected with the torsion block 63. Secondly, the clock spring 601 is installed in the docking box 20 and fixedly connected with the outer wall of the transmission column 60, and is used for resetting the transmission column 60. The transmission disc 61 is rotatably installed at the bottom of the docking box 20, a plurality of transmission blocks 62 are arranged at equal angles on the outer wall of the transmission disc 61, one side of the transmission block 62 is a plane and abuts against the corner of the positioning block 21, and the other side is an inclined surface, which can push the positioning block 21 when rotating. Because one side of the transmission block 62 is a plane, if the rotation direction is incorrect during the rotation of the transmission disc 61, the transmission block 62 will be blocked by the corner of the positioning block 21 and cannot be rotated, so as to facilitate the user to determine whether the reverse rotation is correct. The distance between the two transmission blocks 62 arranged symmetrically is greater than the distance between the inner walls of the positioning blocks 21, and the inclined surface of the transmission block 62 can guide it to abut against the positioning block 21. At the same time, the pull ring 64 is installed at the top of the torsion block 63, which is convenient for pulling the top cover 11.
[0026] For example, the installation mechanism 7 is used to install the isolator 3 into the culture dish body 1, which specifically includes an installation groove 70, a rectangular block 71, a rectangular groove 72, a connecting column 73, a second connecting groove 74, a bolt 75 and a threaded hole 76. The installation groove 70 is arranged in the inner wall of the bottom of the base 10 and is slidably connected with the bottom of the isolation barrel 30, and the opening at the top of the installation groove 70 abuts against and is sealed by the sealing strip 40 on the outer wall of the isolation barrel 30. The rectangular block 71 is integrally installed at the bottom of the isolation barrel 30, and the rectangular groove 72 is arranged in the inner wall of the bottom of the base 10 and communicates with the installation groove 70. Secondly, the connecting column 73 is installed in the rectangular groove 72, the second connecting groove 74 is opened at the bottom of the rectangular block 71 and is in butt joint with the connecting column 73; the bolt 75 is slidingly installed at the bottom of the isolation barrel 30, the threaded hole 76 is opened at the top of the connecting column 73 and is in threaded connection with the bolt 75, so as to realize the fixation of the isolator 3.
[0027] Through the cooperation of the above-mentioned components, when installing, the isolator 3 is first fixed in the base 10 by the installation mechanism 7 to divide the culture chamber, then the top cover 11 is in butt joint with the base 10, and is fixed by the butt joint device 2, the gas supply mechanism 5 drives the inflatable air bag 41 to inflate and cooperate with the sealing strip 40 to form multiple seals; when unlocking, the torsion block 63 is rotated to push the positioning block 21 through the transmission block 62 to release the fixation, so that the top cover 11 can be opened; the culture dish realizes independent cultivation of multiple chambers, has excellent sealing performance, and meets the needs of medical beauty stem cell experiment; because in the process of butt joint and separation of the top cover 11 and the base 10, the top cover 11 can only move up and down under the limitation of the butt joint groove 100, the guide plate 101, the butt joint plate 110, the guide groove 111 and the storage groove 112, damage to the sealing strip 40 caused by rotation of the top cover 11 is avoided, and the risk of wear of the sealing strip 40 in the process of butt joint and separation of the top cover 11 and the base 10 is reduced.
[0028] Before use, the isolator 3 needs to be fixed in the base 10 of the culture dish body 1 by the installation mechanism 7 to realize the division of multiple culture chambers; Firstly, the bottom of the isolation barrel 30 of the isolator 3 is aligned with the installation groove 70 in the inner wall at the bottom of the base 10, the isolation barrel 30 is pushed, the rectangular block 71 at the bottom of the isolation barrel 30 is slid into the rectangular groove 72, and the second connecting groove 74 at the bottom of the rectangular block 71 is in butt joint with the connecting column 73 in the rectangular groove 72, so that the preliminary positioning of the isolator 3 is completed; after positioning, the sealing strip 40 on the outer wall of the isolation barrel 30 is attached to the opening at the top of the installation groove 70 and the inner wall at the bottom of the base 10, and preliminary sealing is formed; It should be noted that in the process of installing the isolator 3 into the base 10, the sealing strip 40 on the outer wall of the isolation plate 31 will be separated from the inner wall of the base 10, and when the isolator 3 starts to enter the base 10, the sealing strip 40 will be extruded by the base 10 and the isolation plate 31, so as to ensure the sealing between the isolation plate 31 and the base 10; Then, the bolt 75 is slidingly installed at the bottom of the isolation barrel 30, and the bolt 75 is rotated to be in threaded connection with the threaded hole 76 at the top of the connecting column 73, so that the isolation barrel 30 and the base 10 are completely fixed; at this time, the isolation plate 31 of the isolator 3 cooperates with the isolation barrel 30 to divide the inside of the culture dish body 1 into multiple independent culture chambers, which can be used for stem cell culture experiments under different conditions respectively; After the installation of the isolator 3 is completed, the top cover 11 is in butt joint with the base 10 and is fixed by the butt joint device 2: The abutting plate 110 of the outer wall of the top cover 11 is aligned with the abutting groove 100 of the base 10, and the top end of the guide plate 101 of the outer wall of the base 10 is slid into the guide groove 111 of the abutting plate 110, the guide groove 111 guides the accurate downward movement of the top cover 11, until the bottom surface of the top cover 11 is attached to the top surface of the base 10, and the sealing gasket at the attachment position preliminarily blocks the external pollution; when continuing to move downward, the guide plate 101 is slid into the receiving groove 112 of the top cover 11, and the preliminary abutment of the top cover 11 and the base 10 is completed; During the downward movement of the top cover 11, the abutting box 20 at the bottom of the top cover 11 is inserted into the isolation barrel 30 in response to the downward movement of the top cover 11, and during the process, the bottom corner of the first connecting groove 22 of the outer wall of the abutting box 20 is in contact with the top arc corner design of the positioning block 21 in the isolation barrel 30; as the abutting box 20 continues to move downward, the inclined surface design of the positioning teeth 23 in the inner wall of the first connecting groove 22 extrudes the positioning block 21, forcing the positioning block 21 to rotate outward, and since the positioning block 21 is rotationally connected to the isolation barrel 30 through a torsional spring, the torsional spring is in a compressed state at this time; when the positioning teeth 23 are aligned with the positioning groove 24 of the positioning block 21, the positioning block 21 is automatically reset under the action of the torsional spring, and at this time, the plane of the positioning teeth 23 and the positioning groove 24 is attached, and the top cover 11 and the base 10 are fixed by the abutting device 2 to prevent loosening; After the top cover 11 and the base 10 are fixed, the sealing mechanism 4 is driven by the gas supply mechanism 5 to realize multiple sealing, thereby ensuring the sterile independence of the culture chamber; Since the protruding part of the sealing strip 40 on the outer wall of the isolation barrel 30 and the isolation plate 31 is on the surface of the part, after the abutment of the top cover 11 and the base 10, the corresponding sealing strip 40 is tightly attached to the inner wall of the base 10 and the inner wall of the top cover 11, forming the first sealing barrier to block the gas exchange between the culture chambers and between the internal and external environments; At the same time, during the downward movement of the top cover 11, the bottom surface of the top cover 11 is attached to the lower pressing ring 52 at the top of the isolation barrel 30 and pushes the lower pressing ring 52 to move downward, so that the lower pressing ring 52 drives the piston ring 51 in the inflation groove 50 to extrude the return spring 520, and the gas in the inflation groove 50 is pressed into the air bag groove 54 of the isolation plate 31 through the gas guide groove 53; after the inflation of the inflatable air bag 41, the inflatable air bag 41 expands outward, protrudes from the outer wall of the isolation plate 31 and is attached to the inner wall of the corresponding top cover 11 and base 10, forming the second sealing, thereby further enhancing the independence of each culture chamber; the gas guide groove 53 communicates with the air bag groove 54 through the air hole 55, thereby ensuring the smooth inflation; When it is necessary to open the culture dish, the abutting device 2 is released by the unlocking device 6; specifically, first, the torsion block 63 at the top of the top cover 11 is rotated, the torsion block 63 drives the transmission column 60 and the transmission disc 61 at the bottom end to rotate synchronously, and at this time, the clockwork spring 601 in the abutting box 20 is twisted to store energy; Meanwhile, the transmission block 62 on the outer wall of the transmission disc 61 rotates with the transmission disc 61, the inclined surface of the transmission block 62 guides the transmission block 62 to contact the inner wall of the positioning block 21, and then the rotation of the transmission block 62 starts to push the positioning block 21 to expand outward, so that the positioning groove 24 of the positioning block 21 is separated from the positioning tooth 23 of the docking box 20, and the locking of the docking device 2 is released; Then, the pull ring 64 is pulled upward, the top cover 11 drives the docking box 20 to separate from the isolation barrel 30, and the guide plate 101 slides back to the guide groove 111 from the storage groove 112, assisting the top cover 11 to be smoothly taken out; after the top cover 11 is removed, the torsion block 63 is loosened, at this time, the elastic force of the clock spring 601 is released, the transmission column 60, the transmission disc 61 and the torsion block 63 are reset, and are waiting for next use; The culture dish realizes parallel development and sterile protection of multiple groups of stem cell experiments through precise cooperation of mechanical structures, and meets strict requirements of medical cosmetic experiments on culture environment stability and independence.
[0029] The basic principles, main features and advantages of the present application are shown and described above. Those skilled in the art should understand that the present application is not limited by the above examples, and the above examples and descriptions in the specification are only preferred examples of the present application and are not intended to limit the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of the claimed present application. The scope of protection of the present application is defined by the appended claims and their equivalents.
[0030] The terms "center", "longitudinal", "transverse", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, which is only a simplified description for the convenience of describing the present application, and does not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the scope of protection of the present application.
[0031] Although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent substitutions for part of the technical features, as long as they are within the spirit and principles of the present application. Any modification, equivalent substitution, improvement, etc. made within the scope of the present application should be included in the scope of protection of the present application.
Claims
1. A medical cosmetology experimental stem cell culture dish comprising a culture dish body (1) consisting of a base (10) and a top cover (11), characterized in that: The culture dish body (1) is provided with a docking device (2), an isolator (3), a sealing mechanism (4), an unlocking device (6) and a mounting mechanism (7), the docking device (2) is used for fixing the connection between the base (10) and the top cover (11), the sealing mechanism (4) is used for sealing the connection between the isolator (3) and the culture dish body (1) and the connection between the base (10) and the top cover (11), the unlocking device (6) is used for releasing the restriction of the docking device (2) on the culture dish body (1), and the mounting mechanism (7) is used for mounting the isolator (3) into the culture dish body (1); The isolator (3) is used for dividing the culture dish body (1) into multiple culture chambers, and the isolator (3) is composed of an isolation barrel (30) connected with the base (10) and an isolation plate (31); The docking device (2) comprises: A docking box (20) mounted at the bottom of the top cover (11) and a positioning block (21) rotatably mounted in the isolation barrel (30), and a first connecting groove (22) corresponding to the positioning block (21) is arranged on the outer wall of the docking box (20); A plurality of positioning teeth (23) are mounted on the inner wall of the first connecting groove (22), a plurality of groups of positioning grooves (24) are formed on the outer wall of the positioning block (21), the top surfaces of the plurality of groups of positioning grooves (24) are flush with the top surfaces of the corresponding positioning teeth (23), the top surfaces of the positioning teeth (23) and the positioning grooves (24) are flat, and the outer wall of the positioning teeth (23) close to the positioning grooves (24) is designed as an inclined surface; The unlocking device (6) comprises a transmission disc (61) rotatably mounted at the bottom of the docking box (20) and a transmission block (62) mounted on the outer wall of the transmission disc (61), the transmission block (62) pushes the positioning block (21) outward during rotation, and the top of the transmission disc (61) is connected with a torsion block (63) located at the top of the top cover (11) and rotatably connected with the top cover (11).
2. The stem cell culture dish for medical and cosmetic experiments according to claim 1, characterized in that: The culture dish body (1) further comprises: A docking groove (100) is symmetrically formed on the outer wall of the base (10), and the docking groove (100) is slidably connected with a docking plate (110) integrally mounted on the outer wall of the top cover (11); A guide plate (101) is integrally mounted on the outer wall of the base (10), a guide groove (111) corresponding to the guide plate (101) is formed on the outer wall of the docking plate (110), and the bottom of the guide groove (111) is connected with a receiving groove (112) formed on the outer wall of the top cover (11).
3. The stem cell culture dish for medical and cosmetic experiments according to claim 2, characterized in that: The outer ends of the docking plate (110) and the guide plate (101) protrude from the top cover (11) and the base (10), the thickness of the top end of the guide plate (101) is less than the thickness of the bottom end, and the inner wall of the guide plate (101) is flush with the inner wall of the guide groove (111); The top surface of the base (10) is flush with the bottom surface of the top cover (11), and the position where the top cover (11) is flush with the base (10) is provided with a sealing gasket.
4. The stem cell culture dish for medical and cosmetic experiments according to claim 1, wherein: A plurality of groups of first connecting grooves (22) are symmetrically mounted on the outer wall of the docking box (20), and the width of the first connecting groove (22) is greater than the width of the positioning block (21). The positioning blocks (21) are equiangularly arranged in the middle of the isolation barrel (30), the inner walls of the positioning blocks (21) are attached to the inner walls of the first connecting grooves (22), and the top sides of the positioning blocks (21) near the side edges of the docking box (20) are designed in arc corners.
5. The stem cell culture dish for medical and cosmetic experiments according to claim 1, wherein: The sealing mechanism (4) comprises sealing strips (40) and inflatable air bags (41) arranged on the outer edges of the isolation barrel (30) and the isolation plate (31), the sealing strips (40) protrude from the isolation barrel (30) and the isolation plate (31), and after the base (10) is connected with the top cover (11), the sealing strips (40) are attached to the inner walls of the base (10) and the top cover (11); The inflatable air bags (41) are arranged on the outer walls of the isolation plate (31), the cross sections of the inflatable air bags (41) are designed in the shape of a Chinese character "fang", and the outer ends of the inflatable air bags (41) do not protrude from the outer walls of the isolation plate (31) when the inflatable air bags (41) are not inflated.
6. The stem cell culture dish for medical and cosmetic experiments according to claim 5, wherein: The isolator (3) is internally provided with a gas supply mechanism (5) corresponding to the inflatable air bags (41), which comprises: The inflation groove (50) is arranged in the inner wall of the isolation barrel (30), the piston ring (51) is slidably arranged in the inflation groove (50), the lower pressing ring (52) is connected to the top of the piston ring (51) and is slidably connected to the isolation barrel (30), the top of the lower pressing ring (52) is located at the top of the isolation barrel (30), and during the connection of the top cover (11) and the base (10), the top surface of the lower pressing ring (52) is attached to the bottom surface of the top cover (11); The reset spring (520) is arranged in the inflation groove (50), and the top of the reset spring (520) is connected to the bottom surface of the inflation groove (50); The gas guide groove (53) is arranged in the inner wall of the isolation plate (31), and the gas guide groove (53) is in communication with the inflation groove (50); The air bag groove (54) is arranged in the outer wall of the gas guide groove (53), and the opening of the air bag groove (54) is covered and sealed by the inflatable air bag (41); A plurality of air holes (55) are arranged in the inner wall of the isolation plate (31), and the two ends of the air holes (55) are in communication with the gas guide groove (53) and the air bag groove (54).
7. The stem cell culture dish for medical and cosmetic experiments according to claim 6, characterized in that: The width of the cross section of the inflation groove (50) is greater than the diameter of the gas guide groove (53), and the diameter of the gas guide groove (53) is greater than the diameter of the air hole (55).
8. The stem cell culture dish for medical and cosmetic experiments according to claim 1, wherein: The unlocking device (6) further comprises: The transmission column (60) is rotatably arranged in the docking box (20) and is rotatably connected to the top cover (11), the bottom end of the transmission column (60) is fixedly connected to the transmission disc (61), and the top end of the transmission column (60) is fixedly connected to the torsion block (63); The clockwork spring (601) is arranged in the inner wall of the docking box (20) and is fixedly connected to the outer wall of the transmission column (60), and is used for resetting the rotated transmission column (60); The pull ring (64) is arranged on the top of the torsion block (63).
9. The stem cell culture dish for medical and cosmetic experiments according to claim 8, characterized in that: The diameter of the transmission disc (61) is smaller than the distance between the inner walls of the positioning blocks (21), and a plurality of transmission blocks (62) are equiangularly arranged on the outer wall of the transmission disc (61), one side of the transmission block (62) is designed in a plane and is attached to the edge of the positioning block (21), and the other side is designed in an inclined surface. The distance between the outer walls of the two mutually symmetrical transmission blocks (62) is greater than the distance between the inner walls of the positioning block (21). When the transmission blocks (62) start to rotate with the transmission disc (61), the inclined surfaces of the transmission blocks (62) guide the transmission blocks (62) to rotate to the state of being attached to the inner walls of the positioning block (21).
10. The stem cell culture dish for medical and cosmetic experiments according to claim 5, wherein: The mounting mechanism (7) comprises: A mounting groove (70) is formed in the inner wall of the bottom of the base (10), the mounting groove (70) is in sliding connection with the bottom of the isolation barrel (30), and the opening at the top of the mounting groove (70) is attached to the sealing strip (40) on the outer wall of the isolation barrel (30); A rectangular block (71) is integrally installed at the bottom of the isolation barrel (30), a rectangular groove (72) corresponding to the rectangular block (71) is formed in the inner wall of the bottom of the base (10), and the rectangular groove (72) is in communication with the mounting groove (70); A connecting column (73) is installed in the rectangular groove (72), a second connecting groove (74) corresponding to the connecting column (73) is formed in the bottom of the rectangular block (71); A bolt (75) is slidingly installed at the bottom of the isolation barrel (30), and a threaded hole (76) in screw connection with the bolt (75) is formed in the top of the connecting column (73).