Saliva glucose test paper

Abstract

The invention relates to the technical field of biomedical detection, in particular to salivary glucose detection test paper which comprises a supporting mechanism, the supporting mechanism comprises a lower shell, an upper shell is connected to the top of the lower shell in an inserted mode, a sample adding hole is formed in the outer surface of the upper shell, an observation hole is formed in the outer surface of the end, away from the sample adding hole, of the upper shell, and the observation hole is communicated with the lower shell. The inner wall of the observation hole is fixedly connected with a transparent plate, a detection mechanism is arranged between the lower shell and the upper shell, the detection mechanism comprises a connecting shell, the connecting shell is clamped in the lower shell, the inner wall of the connecting shell is fixedly connected with a water absorption layer, the upper surface of the water absorption layer is fixedly connected with a reaction layer, and the upper surface of the reaction layer is fixedly connected with a water outlet. An adsorption layer is fixedly connected to the outer surface of the side, close to the sample adding hole, of the reaction layer, large-particle impurities in saliva are sequentially filtered through the filtering layer, the adsorption layer and the reaction layer, mucin and other interferents in the saliva are adsorbed, and therefore interference is eliminated, and the reliability of a detection result is improved.

Description

Technical Field

[0001] This invention relates to the field of biomedical testing technology, and more specifically, to a saliva test strip. Background Technology

[0002] The saliva glucose test strip is a convenient in vitro testing tool that can quickly determine the glucose concentration in a small saliva sample. Users simply need to contact the test strip with saliva and wait for a specified time before comparing the result with a colorimetric card to intuitively obtain the glucose level in the saliva. This test strip is suitable for daily health monitoring and provides a painless and non-invasive preliminary screening reference for people who need to monitor their blood sugar trends. It is especially suitable for home, outdoor, and rapid screening scenarios.

[0003] Because current saliva glucose test strips follow the design concept of blood glucose test strips, they only have a simple sample diffusion layer. When testing for glucose in saliva, untreated saliva is directly introduced into the sample diffusion layer. Since saliva contains various components such as mucin, amylase, food residue particles, and bacteria, these components can irreversibly adsorb or deposit on the surface of the reaction membrane, clogging the micropores on the membrane. This hinders the effective binding of glucose molecules with the reaction enzymes in the test strip, ultimately leading to inaccurate test results. Summary of the Invention

[0004] This invention provides a saliva sugar test strip, which employs a multi-stage process consisting of a filter layer, an adsorption layer, and a reaction layer. The filter layer filters and separates large particulate impurities in saliva, the adsorption layer captures and fixes mucinous interfering substances in saliva, and the reaction layer further removes interfering substances from saliva. This effectively avoids the influence of interfering substances on the detection process, thereby improving the accuracy and reliability of saliva sugar test results. This solves the problem mentioned in the background art, namely that saliva contains various components such as mucin, amylase, food residue microparticles, and bacteria. These components can irreversibly adsorb onto or deposit on the surface of the reaction membrane, causing blockage of the micropores on the membrane, thus hindering the effective binding of glucose molecules and the reaction enzyme, ultimately leading to deviations in the test strip's detection results.

[0005] To achieve the above objectives, a saliva test strip includes a support mechanism, which includes a lower shell, an upper shell inserted into the top of the lower shell, a sample application hole on the outer surface of the upper shell, an observation hole on the outer surface of the upper shell away from the sample application hole, a transparent plate fixedly connected to the inner wall of the observation hole, and a detection mechanism provided between the lower shell and the upper shell. In the above technical solution, the detection mechanism includes a connecting shell, which is snapped into the interior of the lower shell. An absorbent layer is fixedly connected to the inner wall of the connecting shell, a reaction layer is fixedly connected to the upper surface of the absorbent layer, an adsorption layer is fixedly connected to the outer surface of the reaction layer near the sample application hole, a filter layer is fixedly connected to the outer surface of the adsorption layer, and the outer surface of the reaction layer near the observation hole is set as a color development area.

[0006] It is worth noting that the filter layer is the first to come into contact with saliva, which can quickly absorb the dripped saliva sample and achieve lateral diffusion of the saliva sample. At the same time, the filter layer is made of hydrophilic glass fiber non-woven fabric, which can effectively intercept large particles such as food residue particles and shed epithelial cells contained in saliva, thereby achieving preliminary treatment of saliva sample.

[0007] Meanwhile, the adsorption layer is used to capture and immobilize mucins and glycoproteins in saliva, thereby reducing the impact of mucins and glycoproteins on glucose detection results in saliva.

[0008] It is worth noting that the reaction layer contains a substance that can undergo a specific enzymatic reaction with glucose in saliva. After the reaction layer reacts with glucose in saliva, it can generate an insoluble colored precipitate, which will show color in the color development area. Furthermore, the absorbent layer is a porous material layer with high liquid absorption capacity, which is used to absorb and store excess liquid remaining after the detection reaction is completed.

[0009] Preferably, the thickness of the absorbent layer is greater than the thickness of the filter layer, the thickness of the reaction layer is less than the thickness of the filter layer, the reaction layer allows the reaction reagent to fully contact the saliva, the thickness of the adsorption layer is less than the thickness of the reaction layer, and the adsorption layer is used to avoid obstructing the flow rate of the saliva sample.

[0010] Based on the above, a through hole is provided on the outer surface of the observation hole near the sample feeding hole, and a guide groove is provided on the inner wall of the transparent plate near the sample feeding hole.

[0011] Furthermore, the through hole and the guide groove are interconnected. The guide groove is used to guide saliva that is accidentally dripped into the observation hole to the side of the filter layer. The color development area completely covers the visible area below the observation hole, and the area of ​​the color development area is larger than the opening area of ​​the observation hole. The color development area is used to effectively observe the color reaction results of the reaction layer. If the user accidentally drips saliva into the area around the observation hole, this part of the saliva can flow into the guide groove through the through hole. With the guiding effect of the guide groove, the accidentally dripped saliva is guided to the filter layer side of the detection mechanism, thereby effectively avoiding the waste of saliva samples and preventing the test strip from failing due to misoperation.

[0012] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. In this saliva sugar test strip, large particulate impurities in saliva are filtered and interfering substances such as mucin in saliva are adsorbed through a filter layer, an adsorption layer and a reaction layer in sequence, thereby eliminating interference and improving the reliability of the test results.

[0013] 2. In this saliva test strip, by opening through holes and guide grooves on the side wall of the observation hole, if the user accidentally drips saliva into the area around the observation hole, the saliva can flow into the guide groove along the through holes, and then be guided by the guide groove to the filter layer of the detection mechanism, thereby effectively reducing the loss of saliva sample. At the same time, it can also avoid the test strip failure caused by operational errors. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall front structure of the present invention; Figure 2 This is a schematic diagram of the overall frontal exploded structure of the present invention; Figure 3 This is a schematic diagram of the overall front cross-sectional structure of the present invention; Figure 4 For the present invention Figure 3 A magnified structural diagram of A in the middle; Figure 5 This is a schematic cross-sectional view of the detection mechanism of the present invention. Figure 1 ; Figure 6 This is a schematic cross-sectional view of the detection mechanism of the present invention. Figure 2 ; Figure 7 For the present invention Figure 6 A magnified structural diagram of B in the diagram.

[0015] The meanings of the labels in the diagram are as follows: 1. Supporting mechanism; 101. Lower shell; 102. Upper shell; 103. Sample dispensing hole; 104. Observation hole; 105. Transparent plate; 106. Through hole; 107. Guide groove; 2. Detection mechanism; 201. Connecting shell; 202. Water-absorbing layer; 203. Reaction layer; 204. Adsorption layer; 205. Filter layer; 206. Color development area. Detailed Implementation

[0016] The technical solutions of this invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this invention, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without creative effort are within the scope of protection of this invention.

[0017] Saliva contains various components such as mucin, amylase, food residue particles, and bacteria. These components can irreversibly adsorb onto or deposit on the surface of the reaction membrane, causing blockage of the micropores on the surface of the reaction membrane. This can affect the interaction between glucose molecules in saliva and the reaction enzymes in the test strip, thus leading to deviations in the test results.

[0018] Therefore, in view of the above-mentioned problems, the present invention provides a saliva sugar test strip, with reference to Figure 1 As shown, the device includes a support mechanism 1, which includes a lower housing 101. An upper housing 102 is inserted into the top of the lower housing 101. The upper housing 102 and the lower housing 101 cooperate with each other and together form a sealed cavity structure, which can seal and protect the detection mechanism 2 inside, avoiding interference from the external environment. A sample application hole 103 is provided on the outer surface of the upper housing 102. The sample application hole 103 provides guidance for sample application, guiding the user to accurately drop the saliva sample to be tested onto the designated position of the detection mechanism 2, thereby ensuring the accuracy of saliva sample application. An observation hole 104 is provided on the outer surface of the upper housing 102 away from the sample application hole 103. A transparent plate 105 is fixedly connected to the inner wall of the observation hole 104. The transparent plate 105 is made of polycarbonate, which has both high light transmittance and structural rigidity. The detection mechanism 2 is arranged between the lower housing 101 and the upper housing 102. refer to Figure 2 As shown, the detection mechanism 2 includes a connecting shell 201, which is snapped into the interior of the lower shell 101. An absorbent layer 202 is fixedly connected to the inner wall of the connecting shell 201. A reaction layer 203 is fixedly connected to the upper surface of the absorbent layer 202. An adsorption layer 204 is fixedly connected to the outer surface of the reaction layer 203 near the sample application port 103. A filter layer 205 is fixedly connected to the outer surface of the adsorption layer 204. The filter layer 205 is the first to contact the saliva, rapidly absorbing the dripped saliva sample and enabling lateral diffusion of the saliva sample. Meanwhile, the filter layer 205 is made of hydrophilic glass fiber non-woven fabric, which can effectively intercept large particles such as food residue particles and shed epithelial cells in saliva, thereby achieving preliminary processing of saliva samples. The outer surface of the reaction layer 203 near the observation hole 104 is set as the color development area 206. Through the filter layer 205, adsorption layer 204 and reaction layer, large particles in saliva are filtered in sequence, and interfering substances such as mucin in saliva are adsorbed, thereby eliminating interference and improving the reliability of the test results.

[0019] First-time users or elderly users may misuse the device and accidentally drip saliva onto the area surrounding the observation port 104. (Refer to...) Figure 3-4As shown, a through hole 106 is provided on the outer surface of the observation hole 104 near the sample application hole 103, and a guide groove 107 is provided on the inner wall of the transparent plate 105 near the sample application hole 103. The through hole 106 and the guide groove 107 are connected. The guide groove 107 is used to guide saliva that is accidentally dripped into the observation hole 104 to the filter layer 205. If the user accidentally drips saliva into the area around the observation hole 104, this part of the saliva can flow into the guide groove 107 through the through hole 106. With the guiding effect of the guide groove 107, the accidentally dripped saliva is guided to the filter layer 205 of the detection mechanism 2, thereby effectively avoiding the waste of saliva sample and preventing the test strip from failing due to misoperation.

[0020] The color development area 206 completely covers the visible area below the observation hole 104, and the area of ​​the color development area 206 is larger than the opening area of ​​the observation hole 104. The color development area 206 is used to effectively observe the color development reaction results of the reaction layer 203.

[0021] refer to Figure 5-7 As shown, the filter layer 205 is used to absorb and uniformly diffuse saliva, and to trap large particulate impurities in the saliva. The adsorption layer 204 is used to capture and fix mucin and glycoprotein interferences in the saliva. The adsorption layer 204 is used to reduce the influence of mucin and glycoprotein interferences on the glucose detection results in saliva. After the saliva sample is filtered by the filter layer 205, it comes into full contact with the adsorption layer 204. The adsorption layer 204 is made of nitrocellulose membrane, which can efficiently capture and stably fix the mucin in the saliva. Due to its small molecular weight, the free glucose molecules in the saliva can easily penetrate the adsorption layer 204 and continue to diffuse.

[0022] The reaction layer 203 contains substances that can undergo specific enzymatic reactions with glucose in saliva. After the reaction layer 203 reacts with glucose in saliva, it can generate an insoluble colored precipitate, which will then develop color in the color development area 206. The reaction layer 203 contains glucose oxidase and laccase. Glucose oxidase can catalyze the oxidation of glucose in saliva to generate gluconic acid and hydrogen peroxide. Laccase can oxidize and remove a small amount of small molecule reducing interference that may penetrate the adsorption layer 204, effectively eliminating the influence of various interfering factors and improving the accuracy of the color development reaction and the reliability of the detection results.

[0023] The absorbent layer 202 is a porous material layer with high liquid absorption capacity. The absorbent layer 202 is made of high-density cellulose absorbent paper, which can absorb and stably store excess residual liquid, thereby effectively blocking the backflow of waste liquid and avoiding the waste liquid from contaminating the reaction area. At the same time, it improves the stability and accuracy of the color development after detection.

[0024] The thickness of the absorbent layer 202 is greater than the thickness of the filter layer 205, the thickness of the reaction layer 203 is less than the thickness of the filter layer 205, the reaction layer 203 allows the reaction reagent to fully contact the saliva, and the thickness of the adsorption layer 204 is less than the thickness of the reaction layer 203. The adsorption layer 204 is used to avoid obstructing the flow rate of the saliva sample.

[0025] When testing the glucose content in saliva, the saliva sample should first be dripped into a dedicated collection device. The initial small amount of saliva secreted in the mouth should be discarded during the operation. After the saliva has settled naturally, a clear, non-foaming oral fluid should be collected as the valid test sample. Then, a clean dropper is used to draw up the collected saliva sample and slowly drip it into the sample application hole 103 pre-drilled on the outer surface of the upper housing 102. After dripping, the support mechanism 1 should be placed horizontally and calmly so that the saliva sample can gradually come into full contact with the reaction layer 203 in the detection mechanism 2 through its own osmosis, resulting in a colorimetric reaction. Once the colorimetric area 206 shows color development, the colorimetric result of the colorimetric area 206 is compared with a standard colorimetric card to accurately read and determine the actual glucose content in the saliva sample.

[0026] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely preferred examples and are not intended to limit the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A saliva sugar test strip, comprising a support mechanism (1), the support mechanism (1) comprising a lower shell (101), an upper shell (102) inserted into the top of the lower shell (101), a sample application hole (103) formed on the outer surface of the upper shell (102), an observation hole (104) formed on the outer surface of the upper shell (102) away from the sample application hole (103), and a transparent plate (105) fixedly connected to the inner wall of the observation hole (104), characterized in that: A detection mechanism (2) is provided between the lower housing (101) and the upper housing (102). The testing organization (2) includes: A connecting shell (201) is snapped into the interior of the lower shell (101). An absorbent layer (202) is fixedly connected to the inner wall of the connecting shell (201). A reaction layer (203) is fixedly connected to the upper surface of the absorbent layer (202). An adsorption layer (204) is fixedly connected to the outer surface of the reaction layer (203) near the sample application hole (103). A filter layer (205) is fixedly connected to the outer surface of the adsorption layer (204). The outer surface of the reaction layer (203) near the observation hole (104) is set as a color development area (206).

2. The saliva sugar test strip according to claim 1, characterized in that: The filter layer (205) is used to absorb and evenly diffuse saliva, and to trap large particulate impurities in the saliva.

3. The saliva sugar test strip according to claim 1, characterized in that: The adsorption layer (204) is used to capture and fix mucins and glycoproteins in saliva, and the adsorption layer (204) is used to reduce the influence of mucins and glycoproteins on glucose detection results in saliva.

4. The saliva sugar test strip according to claim 1, characterized in that: After the reaction layer (203) reacts with glucose in saliva, the reaction layer (203) can generate an insoluble colored precipitate, which will then be colored in the color development area (206).

5. The saliva sugar test strip according to claim 1, characterized in that: The absorbent layer (202) is a porous material layer with high liquid absorption capacity. The absorbent layer (202) is used to absorb and store excess liquid remaining after the detection reaction is completed.

6. The saliva sugar test strip according to claim 1, characterized in that: The thickness of the absorbent layer (202) is greater than the thickness of the filter layer (205), and the thickness of the reaction layer (203) is less than the thickness of the filter layer (205). The reaction layer (203) allows the reaction reagent to come into full contact with the saliva.

7. The saliva sugar test strip according to claim 1, characterized in that: The thickness of the adsorption layer (204) is less than the thickness of the reaction layer (203), and the adsorption layer (204) is used to avoid obstructing the flow rate of the saliva sample.

8. The saliva sugar test strip according to claim 1, characterized in that: The observation hole (104) has a through hole (106) on the outer surface of the side near the sample feeding hole (103), and the transparent plate (105) has a guide groove (107) on the inner wall of the side near the sample feeding hole (103).

9. The saliva sugar test strip according to claim 8, characterized in that: The through hole (106) and the guide groove (107) are connected. The guide groove (107) is used to guide saliva that is accidentally dripped into the observation hole (104) to one side of the filter layer (205).

10. The saliva sugar test strip according to claim 1, characterized in that: The color development area (206) completely covers the visible area below the observation hole (104), and the area of ​​the color development area (206) is larger than the opening area of ​​the observation hole (104). The color development area (206) is used to effectively observe the color development reaction results of the reaction layer (203).

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