Microorganism sample automatic inspection device and inspection method

Abstract

The invention provides an automatic microbial sample inspection device and method, the device comprises a base, a support column vertically arranged on the base, a sliding sleeve slidably arranged on the support column and a first driving assembly used for driving the sliding sleeve to lift up and down, a rotating seat is rotatably arranged on the sliding sleeve, and a second driving assembly is arranged between the rotating seat and the sliding sleeve. A plurality of needle tubes which are uniformly distributed in the circumferential direction of the supporting column and are used for containing reagents are arranged on the rotating seat, a sample bottle which is vertically arranged on the base is arranged below one needle tube, a detection head is arranged below the sample bottle, and the detection head is connected with a control circuit board; a third driving assembly used for pushing a piston in a needle tube located over the sample bottle is further arranged on the supporting column, and the first driving assembly, the second driving assembly and the third driving assembly are connected with the control circuit board. Due to the fact that the needle tubes are arranged, and the reagents contained in the needle tubes are different, the multiple reagents can be injected into the sample bottles through the device respectively, and diversity of inspection functions is achieved.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of microbiological examination, and relates to a microbiological sample automatic examination device and an examination method. BACKGROUND

[0002] Clinical microbiological examination is an indispensable aid in the fields of disease diagnosis, rational use of antibiotics, hospital infection control and the like of medical institutions. The microbiological sample automatic examination device in the prior art has relatively low examination efficiency, and an examiner often needs to dip reagents multiple times to examine microbiological samples, which wastes a large amount of time, affects observation of the examiner on the microbiological samples, and greatly reduces examination efficiency.

[0003] Therefore, a microbiological sample automatic examination device (authorized publication number CN208414431U) is disclosed in a Chinese patent, which comprises a bottom plate, a support column, a circuit board, a fixing frame, a colorimetric sensor, a fermentation bottle, a bottle plug, a needle, a needle fixing plate, a needle tube, a piston, a nut plate and a screw rod and the like components. The device realizes automatic filling of reagents through a mechanical structure, which reduces the steps of manual multiple dipping of reagents to a certain extent.

[0004] However, the device still has obvious limitations: the design mainly aims at multiple addition of a single reagent, and cannot realize injection of multiple different reagents into a microbiological sample cup in the same examination process. This defect limits the diversity of examination functions, makes it difficult to adapt to complex or multi-step microbiological examination requirements, and greatly restricts the application range. SUMMARY

[0005] The application aims at the above problems existing in the prior art, and provides a microbiological sample automatic examination device supporting automatic and accurate filling of multiple reagents. An examination method for examining microbiological samples by using multiple reagents is also provided.

[0006] The object of the application can be realized by the following technical scheme:

[0007] The microbiological sample automatic examination device comprises a base, a support column vertically arranged on the base, a sliding sleeve slidingly arranged on the support column, and a first driving assembly for driving the sliding sleeve to move up and down, a rotating seat is rotationally arranged on the sliding sleeve, a second driving assembly for driving the rotating seat to rotate is arranged between the rotating seat and the sliding sleeve, a plurality of needle tubes for containing reagents are arranged on the rotating seat and are uniformly distributed in the circumferential direction of the support column, a sample bottle is arranged below one of the needle tubes and vertically arranged on the base, a detection head is arranged below the sample bottle, the detection head is connected with a control circuit board, a third driving assembly for pushing an inner piston of the needle tube located directly above the sample bottle is further arranged on the support column, and the first driving assembly, the second driving assembly and the third driving assembly are respectively connected with the control circuit board.

[0008] Due to the multiple needle tubes, and each needle tube contains different reagents, the device can inject multiple reagents into the sample bottle, and realize the diversity of testing function.

[0009] In the above-mentioned automatic microbial sample testing device, the rotating seat comprises a rotating sleeve sleeved on the sliding sleeve, an upper fixed plate arranged at the upper end of the rotating sleeve, and a lower fixed plate arranged at the lower end of the rotating sleeve, the periphery of the upper fixed plate is provided with a first clamping groove for clamping the flange at the tail end of the needle tube, the first clamping groove is multiple and uniformly distributed circumferentially along the supporting column, the periphery of the lower fixed plate is provided with a second clamping groove for clamping the needle head at the head end of the needle tube, the second clamping groove is multiple and arranged one by one corresponding to the first clamping groove, and the lower end of the needle tube installed in the first clamping groove and the second clamping groove abuts against the lower fixed plate.

[0010] The needle tube is positioned by the upper fixed plate and the lower fixed plate, which ensures that the needle tube can rotate with the rotating seat, and at the same time ensures that the needle tube can rise and fall with the sliding sleeve, and the needle tip on the needle head can pierce the bottle plug at the top end of the sample bottle when the upper needle tube descends with the sliding sleeve.

[0011] In the above-mentioned automatic microbial sample testing device, the first driving structure comprises a first electric push rod fixed on the base, the telescopic end of the first electric push rod extends vertically upward and the upper end is connected with the lower end of the sliding sleeve, and the signal input end of the first electric push rod is connected with the first signal output end of the control circuit board.

[0012] When the telescopic end of the first electric push rod extends, it can drive the sliding sleeve to rise, and when the telescopic end of the first electric push rod retracts, it can drive the sliding sleeve to descend.

[0013] In the above-mentioned automatic microbial sample testing device, the second driving structure comprises a driven gear coaxially fixed on the sliding sleeve, a stepping motor fixed on the rotating seat, and a driving gear arranged on the output shaft of the stepping motor, the driving gear is engaged with the driven gear, and the signal input end of the stepping motor is connected with the second signal output end of the control circuit board.

[0014] When the stepping motor works, it can drive the driving gear to rotate, since the driving gear is engaged with the driven gear and the driven gear is fixed, when the driving gear rotates, it can drive the rotating seat to rotate around the sliding sleeve.

[0015] In the above-mentioned automatic microbial sample testing device, the third driving structure comprises a second electric push rod fixed on the supporting rod, the telescopic end of the second electric push rod extends vertically downward and is located directly above the sample bottle, and the signal input end of the second electric push rod is connected with the third signal output end of the control circuit board.

[0016] When the telescopic end of the second electric push rod extends, it can press the piston rod of the needle tube, so that the piston rod descends, thereby injecting the reagent in the needle tube into the sample bottle.

[0017] In the automatic microbiological sample testing device, a distance sensor is installed in a mounting hole at the lower end of the second electric push rod, and the signal output end of the distance sensor is connected to the signal input end of the control circuit board.

[0018] The distance sensor can detect whether the telescopic end of the second electric push rod abuts against the piston rod of the needle tube, thereby improving the injection accuracy of the reagent.

[0019] In the automatic microbiological sample testing device, a ring-shaped illumination lamp is arranged at the lower part of the rotating seat, and the sample bottle is located below the illumination lamp.

[0020] In the automatic microbiological sample testing device, a positioning recess is arranged on the base for positioning the sample bottle, and a slot is arranged at the bottom of the positioning recess, and the detection head is arranged in the slot. The sample bottle is prevented from directly abutting against the detection head.

[0021] The microbiological sample testing method uses the automatic microbiological sample testing device, and comprises the following steps:

[0022] S1, the first electric push rod raises the sliding sleeve to the highest position, and the telescopic end of the second electric push rod is retracted upward to the highest position;

[0023] S2, the sample bottle containing the microbiological sample is placed in the positioning recess;

[0024] S3, the needle tube containing the reagent is fixed on the rotating seat;

[0025] S4, the stepping motor works, and after the specified needle tube is sent to the upper side of the sample bottle, the stepping motor stops working;

[0026] S5, the first electric push rod drives the sliding sleeve to descend, and after the needle tip pierces the sample bottle, the first driving structure stops working;

[0027] S6, the telescopic end of the second electric push rod descends, and the reagent in the needle tube is accurately dropped into the microbiological sample in the sample bottle;

[0028] S7, the illumination lamp and the detection head are turned on, and the microbiological sample is tested.

[0029] In the microbiological sample testing method, when multiple different reagents need to be injected into the same sample bottle, the following steps are further needed:

[0030] S8, after the first reagent is injected, the telescopic end of the second electric push rod rises to separate from the pressing of the piston rod of the needle tube;

[0031] S9, the first electric push rod drives the sliding sleeve to rise, and the needle tip is withdrawn from the sample bottle.

[0032] S10, the stepper motor starts working, and then stops working after the next syringe is delivered directly above the sample vial;

[0033] S11. Repeat S5-S6 until the next reagent is injected;

[0034] S12. Turn on the light and the detection head to test for microorganisms.

[0035] Compared with the prior art, the present invention has the following advantages:

[0036] By setting up a rotatable multi-needle structure, the device achieves fully automated switching and sample addition of various reagents, significantly expanding the types and scope of testing items and effectively improving the versatility and testing efficiency of the device. It also enables automated and quantitative reagent addition, ensuring precise operation and effectively reducing human error and reagent waste. The entire testing process is programmed and controlled by a control circuit board, with a high degree of automation, greatly reducing manual intervention. This not only makes the operation simple but also reduces the risk of sample contamination. Attached Figure Description

[0037] Figure 1 This is a schematic diagram of an automated microbial sample testing device.

[0038] Figure 2 This is a cross-sectional view of an automated microbial sample testing device.

[0039] Figure 3 This is a partial structural diagram of an automated microbial sample testing device.

[0040] In the diagram, 1 is the base; 2 is the support column; 3 is the sliding sleeve; 41 is the rotating sleeve; 42 is the upper fixing plate; 43 is the lower fixing plate; 44 is the first slot; 45 is the second slot; 5 is the syringe; 6 is the sample bottle; 7 is the detection head; 8 is the first electric push rod; 91 is the driven gear; 92 is the stepper motor; 93 is the driving gear; and 10 is the second electric push rod. Detailed Implementation

[0041] The following are specific embodiments of the present invention, which are described in conjunction with the accompanying drawings. However, the present invention is not limited to these embodiments.

[0042] like Figure 1 The automated microbial sample testing device shown includes a base 1, a support column 2 vertically mounted on the base 1, a sliding sleeve 3 slidably mounted on the support column 2, and a first drive assembly for driving the sliding sleeve 3 to move up and down. Figures 1-3As shown, the first drive structure includes a first electric push rod 8 fixed on the base 1. The telescopic end of the first electric push rod 8 extends vertically upward and its upper end is connected to the lower end of the sliding sleeve 3. When the first electric push rod 8 is working, it can drive the sliding sleeve 3 to move up and down.

[0043] like Figure 2 As shown, a rotating seat is rotatably provided on the sliding sleeve 3. The rotating seat includes a rotating sleeve 41 rotatably sleeved on the sliding sleeve 3, an upper fixing plate 42 located at the upper end of the rotating sleeve 41, and a lower fixing plate 43 located at the lower end of the rotating sleeve 41. Figure 1 and Figure 3 As shown, the upper fixing plate 42 is provided with a first slot 44 for inserting the tail flange of the needle tube 5 around its periphery. There are multiple first slots 44 and they are evenly distributed around the support column 2. The lower fixing plate 43 is provided with a second slot 45 for inserting the needle tip of the needle tube 5 around its periphery. There are multiple second slots 45 and they are set one-to-one with the first slots 44. The lower end of the needle tube 5 installed in the first slots 44 and the second slots 45 abuts against the lower fixing plate 43.

[0044] like Figure 1 and Figure 3 As shown, a total of 8 needles are installed on the rotating seat.

[0045] A second drive assembly for driving the rotating seat to rotate is provided between the rotating seat and the sliding sleeve 3, such as... Figures 1-3 As shown, the second drive structure includes a driven gear 91 coaxially fixed on the sliding sleeve 3, a stepper motor 92 fixed on the rotary seat, and a drive gear 93 located on the output shaft of the stepper motor 92. The drive gear 93 meshes with the driven gear 91. When the stepper motor 92 is working, it can drive the drive gear 93 to rotate. Since the drive gear 93 meshes with the driven gear 91 and the driven gear 91 is fixed, when the drive gear 93 rotates, it can drive the rotary seat to rotate around the sliding sleeve 3.

[0046] Below one of the syringes 5 is a sample vial 6 erected on a base 1. The sample vial 6 is made of transparent material and has a stopper at the top. Below the sample vial 6 is a detection head 7, and the signal output terminal of the detection head 7 is connected to the first signal input terminal of the control circuit board. To improve the detection effect, a ring-shaped illumination lamp (not shown in the figure) is provided at the bottom of the rotating seat, and the sample vial 6 is located below the illumination lamp.

[0047] The detection head 7 can be a color sensor, a camera, etc.

[0048] In order to insert the needle tip into the bottle stopper, such as Figures 1-3 As shown, a third drive assembly is also provided on the support column 2 for pushing the piston inside the needle tube 5 located directly above the sample bottle 6. The third drive structure includes a second electric push rod 10 fixed on the support rod. The telescopic end of the second electric push rod 10 extends vertically downward and is located directly above the sample bottle 6.

[0049] To improve the injection accuracy of the reagent, a mounting hole is provided at the lower end of the telescopic end of the second electric push rod 10. A distance sensor is installed in the mounting hole. The signal output terminal of the distance sensor is connected to the signal input terminal of the control circuit board. The distance sensor can detect whether the telescopic end of the second electric push rod 10 is against the piston rod of the syringe 5.

[0050] The signal input terminal of the first electric push rod 8 is connected to the first signal output terminal of the control circuit board, the signal input terminal of the stepper motor 92 is connected to the second signal output terminal of the control circuit board, and the signal input terminal of the second electric push rod 10 is connected to the third signal output terminal of the control circuit board.

[0051] Since sample bottle 6 is a transparent bottle, for convenient direct testing, such as Figure 2 As shown, a positioning cavity for positioning sample bottle 6 is provided on the base 1, and a groove is provided at the bottom of the positioning cavity, and the detection head 7 is located in the groove.

[0052] Because it has multiple syringes 5, and each syringe 5 contains different reagents, this device can inject various reagents into the sample bottle 6, thereby achieving a variety of testing functions.

[0053] The microbial sample testing method, using this automated microbial sample testing device, includes the following steps:

[0054] S1. The first electric push rod 8 raises the sliding sleeve 3 to the highest position, and the telescopic end of the second electric push rod 10 retracts upward to the highest position.

[0055] S2. Place the sample bottle 6 containing the microbial sample into the positioning cavity;

[0056] S3. Fix the syringe 5 containing the reagent onto the rotating seat;

[0057] S4. Stepper motor 92 operates, and after the designated needle 5 is delivered directly above the sample vial 6, stepper motor 92 stops operating.

[0058] S5. The first electric push rod 8 drives the sliding sleeve 3 to descend, so that the first drive structure stops working after the needle tip pierces the sample bottle 6.

[0059] S6. The telescopic end of the second electric push rod 10 descends, precisely dripping the reagent in the syringe 5 into the microbial sample in the sample bottle 6.

[0060] S7. Turn on the light and the detection head 7 to test for microorganisms.

[0061] When multiple different reagents need to be injected into the same sample bottle 6, the following steps are also required:

[0062] S8. After the first drug is injected, the telescopic end of the second electric push rod 10 rises, so that it is released from the pressure on the piston rod of the needle tube 5.

[0063] S9. The first electric push rod 8 drives the sliding sleeve 3 to rise, causing the needle tip to exit from the sample bottle 6;

[0064] S10, Stepper motor 92 starts working, so that the next syringe 5 is delivered directly above the sample bottle 6, and then stepper motor 92 stops working;

[0065] S11. Repeat S5-S6 until the next reagent is injected;

[0066] S12. Turn on the light and the detection head 7 to test for microorganisms.

[0067] The specific embodiments described herein are merely illustrative of the spirit of the invention. Those skilled in the art to which this invention pertains may make various modifications or additions to the described specific embodiments or use similar methods to substitute them, without departing from the spirit of the invention or exceeding the scope defined by the appended claims.

Claims

1. An automated microbial sample testing device, characterized in that, The device includes a base (1), a support column (2) vertically mounted on the base (1), a sliding sleeve (3) slidably mounted on the support column (2), and a first drive assembly for driving the sliding sleeve (3) to move up and down. A rotating seat is rotatably mounted on the sliding sleeve (3). A second drive assembly for driving the rotating seat to rotate is provided between the rotating seat and the sliding sleeve (3). The rotating seat is provided with a plurality of syringes (5) for holding reagents evenly distributed around the support column (2). A sample bottle (6) is mounted on the base (1) below one of the syringes (5). A detection head (7) is provided below the sample bottle (6). The detection head (7) is connected to a control circuit board. A third drive assembly for pushing the piston inside the syringe (5) located directly above the sample bottle (6) is also provided on the support column (2). The first drive assembly, the second drive assembly, and the third drive assembly are respectively connected to the control circuit board.

2. The automated microbial sample testing device according to claim 1, characterized in that, The rotating seat includes a rotating sleeve (41) rotatably mounted on a sliding sleeve (3), an upper fixing plate (42) located at the upper end of the rotating sleeve (41), and a lower fixing plate (43) located at the lower end of the rotating sleeve (41). The upper fixing plate (42) has a first slot (44) around its periphery for inserting the tail flange of the needle tube (5). There are multiple first slots (44) and they are evenly distributed around the support column (2). The lower fixing plate (43) has a second slot (45) around its periphery for inserting the needle tip of the needle tube (5). There are multiple second slots (45) and they are arranged one-to-one with the first slots (44). The lower end of the needle tube (5) installed in the first slot (44) and the second slot (45) abuts against the lower fixing plate (43).

3. The automated microbial sample testing device according to claim 1, characterized in that, The first drive structure includes a first electric push rod (8) fixed on the base (1). The telescopic end of the first electric push rod (8) extends vertically upward and its upper end is connected to the lower end of the sliding sleeve (3). The signal input end of the first electric push rod (8) is connected to the first signal output end of the control circuit board.

4. The automated microbial sample testing device according to claim 1, characterized in that, The second drive structure includes a driven gear (91) coaxially fixed on a sliding sleeve (3), a stepper motor (92) fixed on a rotating base, and a drive gear (93) located on the output shaft of the stepper motor (92). The drive gear (93) meshes with the driven gear (91), and the signal input terminal of the stepper motor (92) is connected to the second signal output terminal of the control circuit board.

5. The automated microbial sample testing device according to claim 1, characterized in that, The third drive structure includes a second electric push rod (10) fixed on a support rod. The telescopic end of the second electric push rod (10) extends vertically downward and is located directly above the sample bottle (6). The signal input end of the second electric push rod (10) is connected to the third signal output end of the control circuit board.

6. The automated microbial sample testing device according to claim 5, characterized in that, The lower end of the telescopic end of the second electric push rod (10) is provided with a mounting hole, and a distance sensor is installed in the mounting hole. The signal output end of the distance sensor is connected to the signal input end of the control circuit board.

7. The automated microbial sample testing device according to claim 1, characterized in that, The lower part of the rotating seat is provided with a ring-shaped lighting lamp, and the sample bottle (6) is located below the lighting lamp.

8. The automated microbial sample testing device according to claim 1, characterized in that, The base (1) is provided with a positioning cavity for positioning the sample bottle (6), and the bottom of the positioning cavity is provided with a groove, and the detection head (7) is located in the groove.

9. A method for testing microbial samples, characterized in that, The automated microbial sample testing device according to any one of claims 1-8 includes the following steps: S1. The first electric push rod (8) raises the sliding sleeve (3) to the highest position, and the telescopic end of the second electric push rod (10) retracts upward to the highest position; S2. Place the sample bottle (6) containing the microbial sample into the positioning cavity; S3. Fix the syringe (5) containing the reagent onto the rotating seat; S4. The stepper motor (92) operates, and the designated syringe (5) is delivered directly above the sample bottle (6), after which the stepper motor (92) stops operating. S5. The first electric push rod (8) drives the sliding sleeve (3) to descend, so that the needle tip pierces the sample bottle (6) and the first drive structure stops working. S6. The telescopic end of the second electric push rod (10) descends, and the reagent in the syringe (5) is accurately dripped into the microbial sample in the sample bottle (6); S7. Turn on the light and the detection head (7) to test for microorganisms.

10. The method for testing microbial samples according to claim 9, characterized in that, When multiple different reagents need to be injected into the same sample bottle (6), the following steps are also required: S8. After the first drug is injected, the telescopic end of the second electric push rod (10) rises, so that it is released from the pressure on the piston rod of the syringe (5). S9. The first electric push rod (8) drives the sliding sleeve (3) to rise, causing the needle tip to exit from the sample bottle (6); S10, the stepper motor (92) works, and the next syringe (5) is delivered directly above the sample bottle (6) before the stepper motor (92) stops working; S11. Repeat S5-S6 until the next reagent is injected; S12. Turn on the light and the detection head (7) to test for microorganisms.

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