Colloidal gold immunochromatographic test strip for detecting melilotus suaveolens and its preparation method and application
By using Ycf1 protein characteristic markers and colloidal gold immunochromatography, a colloidal gold test strip for rice flower honey was prepared, which solved the problems of complex and costly identification of rice flower honey in the existing technology, and realized rapid and convenient identification of honey types.
CN121499799BActive Publication Date: 2026-05-08BEE RES INST CHINESE ACAD OF AGRI SCI +1
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Patent Information
- Application Number
- CN202610037081.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2026-01-13
- Publication Date
- 2026-05-08
- Estimated Expiration
- 2046-01-13
AI Technical Summary
Technical Problem
Existing technologies cannot quickly and conveniently identify the types of rice honey, and the testing equipment is expensive and the analysis process is complex, which cannot meet the needs of rapid screening and large-scale testing.
Method used
Using Ycf1 protein or its truncated form as a characteristic biomarker, and combined with colloidal gold immunochromatography, a colloidal gold immunochromatographic test strip for rice honey was prepared, and Ycf1 protein-specific monoclonal antibody was used for detection.
Benefits of technology
It enables rapid and accurate identification of rice flower honey, is easy to operate, suitable for on-site testing, and reduces testing costs and time requirements.
✦ Generated by Eureka AI based on patent content.
Abstract
The present application relates to the fields of biotechnology and food detection technology, and discloses a rice flower honey colloidal gold immunochromatographic test strip as well as a preparation method and application thereof. Through proteomic analysis, the Ycf1 protein of chloroplast origin is identified as a specific marker of the plant source in rice flower honey, and a rice flower honey colloidal gold immunochromatographic test strip is developed. The test strip comprises a sample pad, a gold label pad, a nitrocellulose membrane and a water absorption pad which are connected in sequence. The gold label pad is coated with gold-labeled antibodies formed by the combination of a monoclonal antibody against a specific segment of the Ycf1 protein and colloidal gold. The nitrocellulose membrane is provided with a detection line and a quality control line. The detection line is coated with a monoclonal antibody against the Ycf1 protein, and the quality control line is coated with a goat anti-mouse IgG. The test strip is simple to operate, and the detection can be completed within 15 minutes. The test strip has high sensitivity, strong specificity, and the results are intuitive and reliable, and is suitable for the on-site rapid screening and identification of the authenticity of rice flower honey.
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