Method for calculating absorption efficiency of plant pigment

Abstract

The invention belongs to the technical field of plant lighting LEDs, and particularly relates to a plant pigment absorption efficiency calculation method, which comprises the following steps: step 1, determining three types of core calculation parameters including pigment parameters, spectral parameters and correction parameters; 2, calculating the basic absorption efficiency of a single target pigment; 3, calculating the final absorption efficiency of the single target pigment; and 4, if the target pigments are various, calculating the comprehensive absorption efficiency of the nine pigments. According to the invention, through systematic calculation logic, various spectrum parameters and different pigment absorption efficiencies form clear quantitative association, so that scientific researchers and industrial practitioners are helped to quickly establish a logic link of spectrum-pigment absorption-plant response; and a scientific and reliable decision basis is provided for practical applications such as facility agriculture light source research and development, plant factory light environment regulation and control, plant cultivation strategy formulation and the like.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of plant lighting LED, and particularly relates to a plant pigment absorption efficiency calculation method. BACKGROUND

[0002] In the fields of plant cultivation, plant physiology research and facility agriculture, the absorption efficiency of plant pigments to light is a key factor affecting plant photosynthesis, growth and development and quality formation. Photosynthetic pigments such as chlorophyll a, chlorophyll b, beta-carotene, lutein and lycopene in plants, and light-responsive substances such as photosensitive pigments (such as Pr type and Pfr type) and DIN standard crop absorption efficiency, each has specific absorption characteristics to light of different wavelengths.

[0003] The absorption efficiency of plant pigments is affected by multiple factors, mainly including:

[0004] Light quality factor: the degree of absorption of light of different wavelengths (such as ultraviolet light, red, blue and green light in visible light, etc.) by various pigments is significantly different, for example, chlorophyll a has strong absorption to blue-violet light and red light, and beta-carotene has prominent absorption to blue-violet light. Light power ratio, photon ratio, R:B (red light to blue light ratio), R:FR (red light to far red light ratio) and other spectral parameters directly determine the composition of available light resources for pigments.

[0005] Pigment characteristics: the molecular structure and absorption cross section of different pigments are different, for example, the absorption peak wavelengths of chlorophyll a and chlorophyll b are slightly different, which leads to natural differences in their absorption efficiency under the same light environment; at the same time, the content, distribution and binding state of pigments to proteins also affect their actual absorption efficiency.

[0006] Environmental and physiological factors: the growth stage, physiological state (such as nutrient level, stress response) and environmental conditions (such as temperature, humidity) of plants will indirectly affect the synthesis, degradation and activity of pigments, and thus change their absorption efficiency to light.

[0007] However, there are many deficiencies in the current application and research of plant light environment:

[0008] Low matching degree of light quality and pigment absorption: the existing light source design or light environment regulation lacks effective analysis of the absorption efficiency of various pigments of plants, so that the light spectrum of the light source does not match the absorption characteristics of plant pigments, causing waste of light energy and restricting the improvement of plant photosynthetic efficiency and growth quality.

[0009] Multi-pigment absorption efficiency comprehensive evaluation is lacking: there are various photosynthetic and light response pigments in plants, and traditional methods usually only analyze the absorption of a single or a few pigments, which cannot comprehensively and systematically evaluate the absorption efficiency of different pigments under a specific spectrum, and cannot meet the fine requirements of plant physiology research and precision cultivation for light environment optimization.

[0010] Insufficient correlation analysis between spectral parameters and pigment absorption: the quantitative correlation between spectral parameters such as light power proportion, photon proportion, R:B (red light to blue light ratio), R:FR (red light to far red light ratio), color coordinate (ccx, ccy), correlated color temperature (CCT), and color deviation index (Duv) and the absorption efficiency of each pigment is lacking, and researchers and industry practitioners cannot quickly and accurately establish the correlation logic of "spectrum-pigment absorption-plant response", which is not conducive to the optimization of light environment and the formulation of plant cultivation strategies.

[0011] In summary, there is an urgent need for a method that can multi-dimensionally and accurately calculate the absorption efficiency of each pigment in plants, to integrate information such as light power proportion, photon proportion, absorption characteristic curve of each pigment, spectral distribution (SPD), photon spectral distribution (SQD), etc., to realize comprehensive calculation and analysis of the absorption efficiency of chlorophyll a, chlorophyll b, beta-carotene, lutein, lycopene, plant photosynthetic sensitive substances, DIN standard crop absorption efficiency, Pr-type photosensitive pigment, Pfr-type photosensitive pigment, etc., and to provide a scientific basis for plant light environment optimization, light source research and development, and cultivation strategy formulation. SUMMARY

[0012] The purpose of the present application is to overcome the many defects and deficiencies in existing plant pigment absorption efficiency calculation techniques, and to provide a scientific, efficient and practical plant pigment absorption efficiency calculation method.

[0013] To achieve the above purpose, the plant pigment absorption efficiency calculation method provided by the embodiments of the present application comprises:

[0014] Step 1: determining three types of core calculation parameters, the core calculation parameters comprising:

[0015] Pigment parameters: the type of target pigment, and the relative absorption coefficient a(λ) of the target pigment under different wavelengths λ;

[0016] Spectral parameters: the spectral distribution SPD of the target light environment, the spectral distribution SPD covering the wavelength range of 350-800 nm and containing the light radiation power P(λ) corresponding to each wavelength λ;

[0017] Correction parameters: plant growth stage, nitrogen content in plant leaves, plant stress status, ambient temperature, and relative humidity. The ambient temperature range is 20~30℃, and the relative humidity range is 50%~80%.

[0018] Step 2: Calculate the baseline uptake efficiency of a single target pigment, specifically including:

[0019] Step 2.1: Determine the effective wavelength range of the target pigment based on its type;

[0020] Step 2.2: Based on the spectral distribution SPD, sum the optical radiation power P(λ) corresponding to all wavelengths λ within the effective wavelength range to obtain the total optical radiation power. ,Right now ;

[0021] Step 2.3: Based on the pigment parameters and spectral parameters, calculate the actual absorption amount of the target pigment. ,Right now ;

[0022] Step 2.4: According to the formula: Basic absorption efficiency = The basic absorption efficiency of the single target pigment was calculated.

[0023] Step 3: Calculate the final absorption efficiency of the single target pigment, specifically including:

[0024] Step 3.1: Based on the aforementioned correction parameters, determine the physiological correction coefficient and the environmental correction coefficient:

[0025] The physiological correction coefficient C = growth stage coefficient × nitrogen content coefficient × stress coefficient, wherein the growth stage coefficient is taken as 0.95 for seedling stage, 1.0 for growth stage, 1.0 for flowering stage, and 1.05 for fruiting stage; the nitrogen content coefficient is taken as 1.0 for sufficient, 0.9 for deficient, and 0.8 for severe deficiency; and the stress coefficient is taken as 1.0 for no stress, 0.9 for mild stress, 0.8 for moderate stress, and 0.7 for severe stress.

[0026] The environmental correction factor K = temperature coefficient × humidity coefficient, wherein the temperature coefficient is set to 1.0 at 25℃ and linearly decreases to 0.9 when it deviates from the range of 20~30℃; the humidity coefficient is set to 1.0 at 60%~70% and decreases to 0.95 when it deviates from the range of 50%~80%.

[0027] Step 3.2: According to the formula: The final absorption efficiency of the single target pigment is calculated by multiplying the physiological correction factor C by the environmental correction factor K.

[0028] Step 4: if the target pigment is multiple, the comprehensive absorption efficiency of the nine pigments is calculated, including the following steps:

[0029] Step 4.1: determine the synergistic correlation coefficient between the nine target pigments;

[0030] Step 4.2: calculate according to the following formula:

[0031] Comprehensive absorption efficiency = .

[0032] Further, in the step 1, the types of the target pigments include chlorophyll a, chlorophyll b, beta-carotene, lutein, lycopene, Pr-type photosensitive pigment, Pfr-type photosensitive pigment, DIN, 9 photosensitive coefficients or plant pigments proposed by plant photosynthesis sensitivity.

[0033] Further, in the step 2.1, the effective wavelength range is determined according to the following rules: chlorophyll a is 350-800 nm, chlorophyll b is 350-800 nm, beta-carotene is 350-800 nm, lutein is 350-800 nm, lycopene is 350-8000 nm, Pr-type photosensitive pigment is 350-800 nm, Pfr-type photosensitive pigment is 350-800 nm, DIN is 400-700 nm, and plant photosynthesis sensitivity is 350-800 nm.

[0034] Further, the plant pigment absorption efficiency calculation method further comprises step 5: establishing a spectrum-efficiency correlation library, storing the spectrum parameters in step 1 and the final absorption efficiency in step 3 and the comprehensive absorption efficiency in step 4, the spectrum parameters including red light and blue light ratio R:B, correlated color temperature CCT.

[0035] Further, in the step 5, the spectrum parameters further include at least one of light power ratio, photon ratio, color coordinate ccx and ccy, color deviation index Duv, and red light and far red light ratio R:FR.

[0036] The plant pigment absorption efficiency calculation method provided in the embodiments of the present application has at least one of the following technical effects:

[0037] Firstly, the single plant pigment absorption efficiency is accurately calculated to solve the problems of low matching degree of light quality and pigment absorption and lack of accurate calculation means in the prior art. By integrating key information such as light power ratio, photon ratio and pigment absorption characteristic curve, errors caused by incomplete parameter consideration and fuzzy calculation logic are eliminated, and the absorption efficiency of single pigments such as chlorophyll a, chlorophyll b, beta-carotene, lutein and lycopene in a specific spectral environment is accurately quantified to provide basic data support for subsequent light environment optimization.

[0038] Secondly, the problem that the traditional method cannot comprehensively evaluate the multi-pigment absorption efficiency is solved, and the simultaneous analysis of the absorption efficiency of multiple photosynthetic and light-responsive pigments in plants is realized. Considering that chlorophyll, carotenoids, phytochrome (Pr type, Pfr type) and DIN and other pigments in plants act synergistically in the process of photosynthesis and growth and development, the present application aims to break the limitation of single pigment analysis, build a comprehensive calculation system of multi-pigment absorption efficiency, and comprehensively reflect the absorption state of various pigments under different spectral conditions to meet the demand of multi-dimensional data for plant physiology research.

[0039] Thirdly, the short board of insufficient correlation analysis between spectral parameters and pigment absorption efficiency is made up, and a clear corresponding relationship between "spectral parameters-pigment absorption efficiency" is established. In view of the problem that the spectral parameters such as light power ratio, photon ratio, red light to blue light ratio R:B, red light to far red light ratio R:FR, color coordinate (ccx, ccy), correlated color temperature CCT and Duv are disconnected with pigment absorption efficiency in the prior art, the present application forms a clear quantitative correlation between various spectral parameters and different pigment absorption efficiency through systematic calculation logic, helps researchers and industry practitioners quickly establish the logical link of "spectrum-pigment absorption-plant response", and provides scientific and reliable decision basis for practical applications such as facility agriculture light source research and development, plant factory light environment regulation, and plant cultivation strategy formulation. BRIEF DESCRIPTION OF DRAWINGS

[0040] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed in the embodiments or prior art description will be briefly introduced as follows. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0041] Figure 1 The calculation output interface diagram of the plant pigment absorption efficiency calculation method of the embodiments of the present application. DETAILED DESCRIPTION

[0042] In an embodiment of the present application, a plant pigment absorption efficiency calculation method is provided, which comprises:

[0043] Step 1: Determine three types of core calculation parameters, including:

[0044] Pigment parameters: type of target pigment, relative absorption coefficient α(λ) of the target pigment at different wavelengths λ; for example, Tables 1-9 below:

[0045]

[0046]

[0047]

[0048]

[0049] Table 1

[0050]

[0051]

[0052]

[0053] Table 2

[0054]

[0055]

[0056]

[0057]

[0058] Table 3

[0059]

[0060]

[0061]

[0062]

[0063] Table 4

[0064]

[0065]

[0066]

[0067]

[0068] Table 5

[0069]

[0070]

[0071]

[0072] Table 6

[0073]

[0074]

[0075]

[0076] Table 7

[0077]

[0078]

[0079]

[0080]

[0081] Table 8

[0082]

[0083]

[0084]

[0085] Table 9

[0086] Spectral parameters: spectral distribution SPD of the target light environment, which covers a wavelength range of 350~800nm ​​and includes the optical radiation power P(λ) corresponding to each wavelength λ.

[0087] Correction parameters: plant growth stage, nitrogen content in plant leaves, plant stress status, ambient temperature (range 20~30℃), ambient relative humidity (range 50%~80%).

[0088] Step 2: Calculate the baseline uptake efficiency of a single target pigment, specifically including:

[0089] Step 2.1: Determine the effective wavelength range of the target pigment based on the type of target pigment;

[0090] Step 2.2: Based on the spectral distribution SPD, sum the optical radiation power P(λ) corresponding to all wavelengths λ within the effective wavelength range to obtain the total optical radiation power. ,Right now ;

[0091] Step 2.3: Based on the pigment parameters and spectral parameters, calculate the actual absorption amount of the target pigment, i.e., the actual absorption amount. ， Right now ;

[0092] Step 2.4: According to the formula The basic absorption efficiency of the single target pigment was calculated.

[0093] Step 3: Calculate the final absorption efficiency of the single target pigment, specifically including:

[0094] Step 3.1: Based on the aforementioned correction parameters, determine the physiological correction coefficient and the environmental correction coefficient:

[0095] The physiological correction coefficient C = growth stage coefficient × nitrogen content coefficient × stress coefficient, wherein the growth stage coefficient is taken as 0.95 for seedling stage, 1.0 for growth stage, 1.0 for flowering stage, and 1.05 for fruiting stage; the nitrogen content coefficient is taken as 1.0 for sufficient, 0.9 for deficient, and 0.8 for severe deficiency; and the stress coefficient is taken as 1.0 for no stress, 0.9 for mild stress, 0.8 for moderate stress, and 0.7 for severe stress.

[0096] The environmental correction factor K = temperature coefficient × humidity coefficient, wherein the temperature coefficient is set to 1.0 at 25℃ and linearly decreases to 0.9 when it deviates from the range of 20~30℃; the humidity coefficient is set to 1.0 at 60%~70% and decreases to 0.95 when it deviates from the range of 50%~80%.

[0097] Step 3.2: According to the formula The final absorption efficiency of the single target pigment is calculated.

[0098] Step 4: If there are multiple target pigments, calculate the overall absorption efficiency of the nine pigments, specifically including:

[0099] Step 4.1: Determine the synergistic correlation coefficients among the nine target pigments;

[0100] Step 4.2: Calculate using the following formula:

[0101] .

[0102] Further, in step 1, the target pigment types include chlorophyll a, chlorophyll b, β-carotene, xanthophyll, lycopene, Pr-type photosensitive pigment, Pfr-type photosensitive pigment, DIN, and the nine photosynthetic sensitivity coefficients or plant pigments proposed by the plant photosynthetic sensitivity assay.

[0103] Further, in step 2.1, the effective wavelength range is determined according to the following rules: chlorophyll a is 350~800nm, chlorophyll b is 350~800nm, β-carotene is 350~800nm, xanthophyll is 350~800nm, lycopene is 350~8000nm, Pr-type phytochrome is 350~800nm, Pfr-type phytochrome is 350~800nm, DIN is 400~700nm, and the photosynthetic sensitivity of plants is 350-800nm.

[0104] Furthermore, the plant pigment absorption efficiency calculation method of the present invention embodiment further includes step 5: establishing a spectrum-efficiency correlation library, and storing the spectral parameters in step 1 in association with the final absorption efficiency in step 3 and the comprehensive absorption efficiency in step 4. The spectral parameters include the red light to blue light ratio R:B and the correlated color temperature CCT.

[0105] Furthermore, the spectral parameters in step 5 also include at least one of the following: light power ratio, photon ratio, chromaticity coordinates ccx and ccy, color deviation index Duv, and red light to far-red light ratio R:FR.

[0106] in: .

[0107] The above , , It is a discrete CIE 1931 standard observer function, with a summation range of 380nm-780nm, and wavelength intervals. It can be 1nm, 5nm, or 10nm; that is:

[0108] .

[0109] The values ​​of K6 to K0 are shown in Table 10 below:

[0110]

[0111] Table 10

[0112] Formula for calculating the proportion of optical power:

[0113] .

[0114] The formula for calculating the photon percentage is as follows:

[0115] .

[0116] Where NA is Avogadro's constant. h is Planck's constant = .

[0117] Example 1:

[0118] The spectral distribution SPD of the target light environment is shown in Table 11 below. The spectral distribution SPD covers a wavelength range of 350~800nm ​​and includes the optical radiation power P(λ) corresponding to each wavelength λ.

[0119]

[0120]

[0121]

[0122]

[0123] Table 11

[0124] The corrected parameters are shown in Table 12 below:

[0125]

[0126] Table 12

[0127] in:

[0128] 1. Temperature 22℃;

[0129] 2. Humidity 65%RH;

[0130] 3. Growth stage: Seedling stage;

[0131] 4. Nitrogen content: Deficient;

[0132] 5. Coercion: No coercion.

[0133] According to the calculation principle: the physiological correction coefficient C = 0.81, the environmental correction coefficient K = 1.00, and the physiological-environmental coordination coefficient is 81%.

[0134] Overall absorption rate: 31.46%.

[0135]

[0136] Table 13

[0137] Table 13 above shows the calculated utilization rate (absorption efficiency) of nine plant pigments.

[0138] Table 14 below shows some calculation tables for light source parameters:

[0139]

[0140] Table 14

[0141] The calculation output interface of the plant pigment absorption efficiency calculation method of this invention is as follows:Figure 1 As shown. Thus, it can be concluded that:

[0142] The advantage of the plant pigment absorption efficiency calculation method in this invention is that it achieves multi-dimensional and accurate calculation of plant pigment absorption efficiency, solving the problems of low matching degree, single evaluation, and insufficient correlation in traditional methods. Specifically, it achieves:

[0143] 1. Precise absorption efficiency of single pigments.

[0144] It integrates pigment parameters, spectral parameters, and correction parameters to eliminate errors caused by incomplete parameter considerations and ambiguous calculation logic. It can accurately quantify the absorption efficiency of single pigments such as chlorophyll a and β-carotene under specific spectra, providing reliable basic data for light environment optimization.

[0145] 2. Comprehensive absorption efficiency of multiple pigments.

[0146] Breaking away from the limitations of traditional single or limited pigment analysis, this study covers nine key photosynthetic and light-responsive pigments. It constructs a multi-pigment synergistic absorption efficiency calculation system to comprehensively reflect the absorption status of various pigments under different spectra, meeting the multi-dimensional data needs of plant physiological research.

[0147] 3. The correlation between spectrum and efficiency is clarified.

[0148] Establish a quantitative correlation between spectral parameters (such as R:B, CCT, and light power ratio) and pigment absorption efficiency. This will help researchers and practitioners quickly build a logical link between "spectrum-pigment absorption-plant response," providing a basis for decision-making in light source development and light environment regulation.

[0149] 4. Adapt to real-world application scenarios.

[0150] The revised parameters incorporate actual environmental and physiological factors such as plant growth stage, nitrogen content, temperature, and humidity, making the calculation results more consistent with actual cultivation practices.

[0151] It supports the establishment of a spectrum-efficiency correlation library, which facilitates continuous optimization of light environment and cultivation strategies.

[0152] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for calculating the absorption efficiency of plant pigments, characterized in that, include: Step 1: Determine three types of core calculation parameters, including: Pigment parameters: type of target pigment, relative absorption coefficient α(λ) of target pigment at different wavelengths λ. Spectral parameters: spectral distribution SPD of the target light environment, which covers a wavelength range of 350~800nm ​​and includes the optical radiation power P(λ) corresponding to each wavelength λ. Correction parameters: plant growth stage, nitrogen content in plant leaves, plant stress status, ambient temperature, and relative humidity. The ambient temperature range is 20~30℃, and the relative humidity range is 50%~80%. Step 2: Calculate the baseline uptake efficiency of a single target pigment, specifically including: Step 2.1: Determine the effective wavelength range of the target pigment based on its type; Step 2.2: Based on the spectral distribution SPD, sum the optical radiation power P(λ) corresponding to all wavelengths λ within the effective wavelength range to obtain the total optical radiation power. ,Right now ; Step 2.3: Based on the pigment parameters and spectral parameters, calculate the actual absorption amount of the target pigment. ,Right now ; Step 2.4: According to the formula: Basal absorption efficiency = The basic absorption efficiency of the single target pigment was calculated. Step 3: Calculate the final absorption efficiency of the single target pigment, specifically including: Step 3.1: Based on the aforementioned correction parameters, determine the physiological correction coefficient and the environmental correction coefficient: The physiological correction coefficient C = growth stage coefficient × nitrogen content coefficient × stress coefficient, wherein the growth stage coefficient is taken as 0.95 for seedling stage, 1.0 for growth stage, 1.0 for flowering stage, and 1.05 for fruiting stage; the nitrogen content coefficient is taken as 1.0 for sufficient, 0.9 for deficient, and 0.8 for severe deficiency; and the stress coefficient is taken as 1.0 for no stress, 0.9 for mild stress, 0.8 for moderate stress, and 0.7 for severe stress. The environmental correction factor K = temperature coefficient × humidity coefficient, wherein the temperature coefficient is set to 1.0 at 25℃ and linearly decreases to 0.9 when it deviates from the range of 20~30℃; the humidity coefficient is set to 1.0 at 60%~70% and decreases to 0.95 when it deviates from the range of 50%~80%. Step 3.2: According to the formula: The final absorption efficiency of the single target pigment is calculated by multiplying the physiological correction factor C by the environmental correction factor K. Step 4: If there are multiple target pigments, calculate the overall absorption efficiency of the nine pigments, specifically including: Step 4.1: Determine the synergistic correlation coefficients among the nine target pigments; Step 4.2: Calculate using the following formula: Overall absorption efficiency = .

2. The method for calculating the absorption efficiency of plant pigments according to claim 1, characterized in that, In step 1, the types of target pigments include chlorophyll a, chlorophyll b, β-carotene, xanthophyll, lycopene, Pr-type photosensitive pigment, Pfr-type photosensitive pigment, DIN, or plant photosynthesis sensitive pigment.

3. The method for calculating the absorption efficiency of plant pigments according to claim 2, characterized in that, In step 2.1, the effective wavelength range is determined according to the following rules: chlorophyll a is 350~800nm, chlorophyll b is 350~800nm, β-carotene is 350~800nm, xanthophyll is 350~800nm, lycopene is 350~8000nm, Pr-type phytochrome is 350~800nm, Pfr-type phytochrome is 350~800nm, DIN is 400~700nm, and plant photosynthesis sensitivity is 350-800nm.

4. The method for calculating the absorption efficiency of plant pigments according to claim 1, characterized in that, It also includes step 5: establishing a spectrum-efficiency correlation library, which links and stores the spectral parameters in step 1 with the final absorption efficiency in step 3 and the comprehensive absorption efficiency in step 4. The spectral parameters include the red to blue light ratio R:B and the correlated color temperature CCT.

5. The method for calculating the absorption efficiency of plant pigments according to claim 4, characterized in that, In step 5, the spectral parameters further include at least one of the following: light power ratio, photon ratio, chromaticity coordinates ccx and ccy, color deviation index Duv, and red light to far-red light ratio R:FR.

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