Portable microfluid bubble manual removing device

Abstract

The invention discloses a portable microfluid bubble manual removal device which comprises a first exhaust part, a second exhaust part, a sample pool and an ultrasonic bath, the sample pool is filled with a solution, and the sample pool is arranged in the ultrasonic bath; the first exhaust part comprises a first injector, a first pipeline and a first air collecting bag; the second exhaust part comprises a second injector, a second pipeline and a second air collecting bag; the first air collecting bag and the second air collecting bag each comprise a hollow bag cavity and a shell, each hollow bag cavity comprises an upper bag cavity part, a middle bag cavity part and a lower bag cavity part which are sequentially connected, and the upper bag cavity parts, the middle bag cavity parts and the lower bag cavity parts are of an integrated structure. In the structural design of the first air collecting bag and the second air collecting bag, the diameter of the middle bag cavity part obtained when the diameter of the middle bag cavity part serves as the characteristic length and the diameter of the middle bag cavity part obtained when the liquid level height in the hollow bag cavity serves as the characteristic length are met at the same time, and it is ensured that bubbles in a sealed fluid loop can be effectively discharged; and bubbles on the wall of each component of the fluid loop are eliminated.

Description

Technical Field

[0001] This invention relates to the field of microfluidics, and more specifically, to a portable microfluidic bubble manual removal device. The invention may also be titled "Portable Microfluidic Bubble Removal Device". Background Technology

[0002] Microfluidics is a rapidly developing interdisciplinary field with wide applications in biomedical engineering, chemical analysis, and materials science. However, the presence of bubbles often causes numerous problems during microfluidic experiments. Bubble formation is mainly due to several factors: desolvation of dissolved gases in the fluid, gas exchange between the fluid and the walls of the experimental equipment, gas infiltration at pipe connections, and shearing forces acting on the liquid as it flows through microchannels. Because the size and distribution of bubbles are highly random, and they easily become trapped in microchannels and fluid chambers, their impact on experimental results is significant.

[0003] First, the presence of bubbles significantly affects the accuracy of optical detection. In microscopic observation and laser scattering experiments, bubbles in the sample cell strongly scatter the incident light, causing fluctuations in the scattered light signal. This strong scattering signal interferes with and masks the particle's scattering signal, thus affecting the accuracy and reliability of experimental data. For example, in fluid particle analysis, bubble scattering can drown out the actual particle signal with noise, increasing the complexity of data processing. Furthermore, the movement and changes in bubbles can destabilize the laser scattering signal, further affecting the repeatability and accuracy of measurements.

[0004] Secondly, the impact of air bubbles on the quality of microscopic imaging cannot be ignored. During microscopic observation, air bubbles cause uneven light intensity distribution in the observation area, resulting in bright spots and shadows in the image. This uneven light intensity distribution blurs the details of the sample, reduces the resolution and contrast of the microscopic image, and affects the effectiveness of image analysis and processing. Especially in high-precision microscopic manipulation and image analysis, the presence of air bubbles can seriously affect the accuracy of observation results, and thus the reliability of experimental conclusions.

[0005] To address the need for bubble removal in microfluidic systems, existing solutions include: 1) designing the geometry of microfluidic channels so that bubbles are guided to specific areas and discharged from the system during flow.

[0006] 2) The inner walls of the microfluidic channels are treated with hydrophilic or hydrophobic agents to control bubble formation and movement. 3) Defoamers are added to the fluid to reduce the surface tension of bubbles, causing them to break down or merge into larger bubbles that are easier to expel. These methods require specialized structural design and chemical treatment of the fluid channels, increasing the complexity of the experiments and the uncertain impact of these treatments on the compatibility of experimental samples. On the other hand, conventional large-scale gas-liquid separation techniques, such as cyclone separation, filtration separation, and fiber coalescence separation, often require large equipment volumes and fluid throughput, making them ineffective when fluid pipelines are narrow and liquid volumes are small.

[0007] Prior art 1 (Application No.: 202411367910.3; Application Date: 2024.9.29) discloses a device and method for removing air bubbles from a micro-sealed pipeline. See [link to previous document]. Figure 1 As shown, Figure 1 This is a schematic diagram of a device and method for removing air bubbles from a micro-closed tubing, as provided in prior art 1. The solution includes a first air-collecting bladder 2', a second air-collecting bladder 4', and an electrically operated injection structure for fixing and pushing the syringe. A filter membrane or wire mesh is sandwiched between the two housing parts of the first air-collecting bladder 2' and the second air-collecting bladder 4' to remove gas from the solution. Each electrically operated injection structure includes a drive motor assembly, a lead screw, a guide rod, a push rod holder for fixing or releasing the push rod, and a syringe holder for fixing the syringe. The aforementioned first air-collecting bladder 2', second air-collecting bladder 4', and... The electric liquid injection structure has the following problems: First, it does not limit the diameter of the first air-collecting bladder 2' and the second air-collecting bladder 4' and the inner diameter of the pipeline, which cannot ensure the exhaust of the first air-collecting bladder 2' and the second air-collecting bladder 4', thus making it impossible to ensure that air bubbles in the micro-sealed pipeline can be effectively discharged; Second, the electric liquid injection structure involves many parts and occupies a large space. At the same time, because the first air-collecting bladder 2', the second air-collecting bladder 4' and the electric liquid injection structure involve many parts, once a failure occurs, the disassembly and replacement of the faulty parts requires breaking through multiple assembly steps, thus causing inconvenience in replacing parts.

[0008] Prior art 2 (Publication No.: US20220260181A1, Publication Date: August 18, 2022) discloses a self-sealing venting fluid system, including a fluid conduit with an inner surface and a fluid check valve. The fluid check valve is used to vent gas from the fluid system. The fluid check valve is installed inside the fluid conduit and forms a fluid seal with the inner surface of the fluid conduit, thereby dividing the fluid conduit into an upstream side that accommodates gas and liquid, and a downstream side that receives gas. This solution is suitable for scenarios requiring precise control of the rheological properties of fluids (such as blood) for measurement (such as coagulation function testing and early diagnosis of sepsis). In specific operation, when a liquid containing gas or a gas-liquid mixture is injected, the gas is automatically vented. After the liquid is filled, the material naturally forms a seal, and no further intervention is required, which is a "passive" function. This solution can only remove gas from gas-liquid mixtures, but cannot remove air bubbles on the inner walls of fluid system components (such as sample cells, connecting pipes, syringes, etc.). It cannot be applied to optical detection interference caused by air bubbles in microfluidic experiments, that is, it cannot be applied to sealing fluid loops.

[0009] 1. It cannot be guaranteed that air bubbles in the miniature sealed pipeline can be effectively discharged and that air bubbles on the walls of each component in the fluid circuit can be removed; 2. Because the first air collecting bladder 2', the second air collecting bladder 4' and the electric liquid injection structure in the prior art 1 involve a large number of components, once damage occurs, the disassembly and replacement of the faulty components requires breaking through multiple assembly steps, which leads to the problem of inconvenience in component replacement. Therefore, how to solve the above two problems has become a technical problem that urgently needs to be solved in this field. Summary of the Invention

[0010] In view of this, the present invention provides a portable microfluidic bubble manual removal device to solve the following technical problems: 1. It is impossible to ensure that bubbles in the micro-sealed pipeline can be effectively removed and bubbles on the walls of each component of the fluid circuit can be removed; 2. Because the first air collecting bladder 2', the second air collecting bladder 4' and the electric liquid injection structure in the prior art 1 involve a large number of components, once damaged, the disassembly and replacement of the faulty components requires breaking through multiple assembly steps, which leads to the problem of inconvenience in replacing components.

[0011] This application provides a portable microfluidic bubble manual removal device, including a first venting component, a second venting component, a sample cell, and an ultrasonic bath. The first venting component and the second venting component are arranged in a mirror-symmetrical manner. The sample cell contains a solution and is disposed in the ultrasonic bath.

[0012] The first exhaust device includes a first syringe, a first tubing, and a first gas collecting bladder. The first syringe and the first gas collecting bladder are placed vertically. The first tubing includes a first sub-tubing and a second sub-tubing that are connected. The second exhaust device includes a second syringe, a second tubing, and a second gas collecting bladder. The second syringe and the second gas collecting bladder are placed vertically. The second tubing includes a third sub-tubing and a fourth sub-tubing that are connected.

[0013] Both the first syringe and the second syringe include a hollow cylindrical syringe and a plunger that matches the syringe. The plunger is a stepped cylindrical rod structure. The plunger is sealed to the inner wall of the syringe through a piston. The rod body and piston connection end of the plunger are located inside the syringe. The pusher extension of the plunger extends to the outside of the syringe. The plunger drives the piston to slide along the axis of the syringe.

[0014] The syringe barrel of the first syringe is connected to the end of the first gas collecting bladder near the first syringe via a first sub-tube, the end of the first gas collecting bladder away from the first syringe is connected to a second sub-tube, and the end of the second sub-tube away from the first gas collecting bladder is inserted into the upper middle part of the solution in the sample cell; the syringe barrel of the second syringe is connected to the end of the second gas collecting bladder near the second syringe via a third sub-tube, the end of the second gas collecting bladder away from the second syringe is connected to a fourth sub-tube, and the end of the fourth sub-tube away from the second gas collecting bladder is inserted into the lower middle part of the solution in the sample cell;

[0015] Both the first and second air-collecting bladders include a hollow bladder cavity and a cylindrical shell surrounding the hollow solution portion. The hollow bladder cavity includes an upper bladder cavity, a middle bladder cavity, and a lower bladder cavity connected in sequence. The upper and lower bladder cavities are semi-circular or conical in shape, and the middle bladder cavity is circular in shape. The upper, middle, and lower bladder cavities are an integral structure. The first sub-tube near the first air-collecting bladder and the third sub-tube near the second air-collecting bladder are respectively threaded to the upper bladder cavity at the end away from the middle bladder cavity. The second sub-tube near the first air-collecting bladder and the fourth sub-tube near the second air-collecting bladder are respectively threaded to the lower bladder cavity at the end away from the middle bladder cavity.

[0016] The diameter of the central cystic cavity satisfies the following relationship:

[0017] and

[0018] In the formula, D DD represents the diameter of the central cyst cavity, obtained when the diameter of the central cyst cavity is used as the characteristic length. H Fr represents the diameter of the central cavity portion, calculated with the liquid level height H within the hollow cavity as the characteristic length. D Fr represents the Froude number calculated when the diameter of the central cystic portion is used as the characteristic length. H The Froude number is calculated with the liquid level height H inside the hollow cavity as the characteristic length, g represents the acceleration due to gravity, and π represents the acceleration due to gravity. 2 It is the square of pi, v1 represents the fluid velocity of the first, second, third and fourth sub-pipes, and A1 represents the cross-sectional area of ​​the first, second, third and fourth sub-pipes.

[0019] Compared with the prior art, the portable microfluidic bubble manual removal device provided by the present invention achieves at least the following beneficial effects:

[0020] First, in the structural design of the first and second gas collecting bladders, it is required that the diameter of the central bladder cavity, obtained when the diameter of the central bladder cavity is used as the characteristic length, and the diameter of the central bladder cavity, obtained when the liquid level height inside the hollow bladder cavity is used as the characteristic length, are simultaneously satisfied. This ensures the venting effect of the first and second gas collecting bladders and also allows them to be used in sealed fluid pipelines of different specifications. Through the synergy between the first venting component, the second venting component, the sample cell, and the ultrasonic bath, it is possible not only to ensure that air bubbles in the sealed fluid circuit can be effectively vented, but also to remove air bubbles from the walls of each component in the fluid circuit. This solves the problem of not being able to ensure that air bubbles in the micro-sealed pipeline can be effectively vented and to remove air bubbles from the walls of each component in the fluid circuit.

[0021] Secondly, compared to the prior art 1 which uses an electric injection structure to drive the syringe and separate first and second air-collecting bladders, the present invention eliminates the filter membrane or wire mesh of the first and second air-collecting bladders, as well as the electric injection structure used to fix and push the syringe. In this embodiment, the first and second air-collecting bladders are integrated structures, with the upper and lower ends of the first air-collecting bladder directly assembled to the first and second sub-tubules, respectively, and the upper and lower ends of the second air-collecting bladder assembled to the third and fourth sub-tubules, respectively. Compared to the prior art 1, this embodiment has fewer parts, making it easier to replace parts (e.g., no need to replace the filter, wire mesh, electric injection structure, etc.), and the overall space occupied is small. It can also be applied to sealed fluid circuits. In other words, it solves the problem in the prior art 1 where the first air-collecting bladder, second air-collecting bladder, and electric injection structure involve many parts, and once damaged, the disassembly and replacement of faulty parts requires breaking through multiple assembly steps, resulting in inconvenient parts replacement.

[0022] Of course, any product implementing this invention does not necessarily need to achieve all of the technical effects described above at the same time.

[0023] Other features and advantages of the invention will become clear from the following detailed description of exemplary embodiments of the invention with reference to the accompanying drawings. Attached Figure Description

[0024] The accompanying drawings, which are incorporated in and form part of this specification, illustrate embodiments of the invention and, together with their description, serve to explain the principles of the invention.

[0025] Figure 1 This is a schematic diagram of the structure of a device and method for removing air bubbles from a micro-closed pipeline provided by prior art 1;

[0026] Figure 2 This is a schematic diagram of the portable microfluidic bubble manual removal device provided by the present invention;

[0027] Figure 3 This is a schematic diagram of the structure of the first or second air collection bag provided by the present invention;

[0028] Figure 4 This is a cross-sectional schematic diagram of the first or second air-collecting bag provided by the present invention;

[0029] Figure 5 This is a schematic diagram of the portable microfluidic bubble manual removal device provided by the present invention in use;

[0030] Figure 6 This is a schematic diagram of the structure of the three-way valve provided by the present invention;

[0031] Figure 7 This is a schematic diagram of the structure of the first syringe or the second syringe provided by the present invention;

[0032] Figure 8 This is a schematic diagram of the structure of the syringe holder provided by the present invention. Detailed Implementation

[0033] Various exemplary embodiments of the present invention will now be described in detail with reference to the accompanying drawings. It should be noted that, unless otherwise specifically stated, the relative arrangement, numerical expressions, and values ​​of the components and steps set forth in these embodiments do not limit the scope of the invention.

[0034] The following description of at least one exemplary embodiment is merely illustrative and is in no way intended to limit the invention or its application or use.

[0035] Techniques, methods, and equipment known to those skilled in the art may not be discussed in detail, but where appropriate, such techniques, methods, and equipment should be considered part of the specification.

[0036] In all the examples shown and discussed herein, any specific values ​​should be interpreted as merely exemplary and not as limitations. Therefore, other examples of exemplary embodiments may have different values.

[0037] It should be noted that similar labels and letters in the following figures indicate similar items; therefore, once an item is defined in one figure, it does not need to be discussed further in subsequent figures.

[0038] See Figures 1 to 5 As shown, Figure 2 This is a schematic diagram of the portable microfluidic bubble manual removal device provided by the present invention; Figure 3 This is a schematic diagram of the structure of the first or second air collection bag provided by the present invention; Figure 4 This is a cross-sectional schematic diagram of the first or second air-collecting bag provided by the present invention; Figure 5 This is a schematic diagram illustrating the usage of the portable microfluidic bubble manual removal device provided by the present invention. Figure 2 This presents the structural components of a portable microfluidic bubble manual removal device. Figure 5 Supplementary Explanation Figure 2 In practical use, the assembly relationship between the second and fourth sub-pipelines and the sealed fluid circuit is as follows: Figure 2 and Figure 5 In the diagram, 'a' represents the ultrasonic bath level line. The ultrasonic bath level line not only ensures that the liquid completely submerges the sample cell, allowing ultrasonic energy to be uniformly transmitted to the sample and avoiding weakened ultrasonic cavitation effect and insufficient sample treatment due to insufficient liquid level, but also keeps the ultrasonic environment (liquid layer thickness, energy transfer conditions) stable for each sample treatment, reducing experimental / process errors. This embodiment provides a portable microfluidic bubble manual removal device, including a first venting component 1, a second venting component 2, a sample cell 3, and an ultrasonic bath 4. The first venting component 1 and the second venting component 2 are arranged in a mirror-symmetrical manner. The sample cell 3 contains a solution and is placed inside the ultrasonic bath 4.

[0039] The first exhaust component 1 includes a first syringe 11, a first tubing 12, and a first air collecting bladder 13. The first syringe 11 and the first air collecting bladder 13 are placed in a vertical position. The first tubing 12 includes a first sub-tubing 121 and a second sub-tubing 122 that are connected. The second exhaust component 2 includes a second syringe 21, a second tubing 22, and a second air collecting bladder 23. The second syringe 21 and the second air collecting bladder 23 are placed in a vertical position. The second tubing 22 includes a third sub-tubing 221 and a fourth sub-tubing 222 that are connected.

[0040] Both the first syringe 11 and the second syringe 21 include a hollow cylindrical syringe 110 and a push rod 111 that matches the syringe 110. The push rod 111 has a stepped cylindrical rod structure. The push rod 111 is sealed and fitted to the inner wall of the syringe 110 through the piston 112. The rod body and piston connection end of the push rod 111 and the piston 112 are all located inside the syringe. The push rod 111 extends to the outside of the syringe. The push rod 111 drives the piston 112 to slide along the axis of the syringe.

[0041] The syringe 110 of the first syringe 11 is connected to the end of the first gas collecting bladder 13 near the first syringe 11 via a first sub-tube 121. The end of the first gas collecting bladder 13 away from the first syringe 11 is connected to a second sub-tube 122. The end of the second sub-tube 122 away from the first gas collecting bladder 13 is inserted into the upper middle part of the solution in the sample cell 3. The syringe 110 of the second syringe 21 is connected to the end of the second gas collecting bladder 23 near the second syringe 21 via a third sub-tube 221. The end of the second gas collecting bladder 23 away from the second syringe 21 is connected to a fourth sub-tube 222. The end of the fourth sub-tube 222 away from the second gas collecting bladder 23 is inserted into the lower middle part of the solution in the sample cell 3.

[0042] Both the first airbag 13 and the second airbag 23 include a hollow cavity 131 and a cylindrical shell 132 surrounding the hollow solution portion. The hollow cavity 131 includes an upper cavity portion 1311, a middle cavity portion 1312, and a lower cavity portion 1313 connected in sequence. The upper cavity portion 1311 and the lower cavity portion 1313 are semi-circular or conical in shape, while the middle cavity portion 1312 is circular in shape. The parts 1313 are integrated into one structure; the first sub-tube 121 near the first air collecting bladder 13 and the third sub-tube 221 near the second air collecting bladder 23 are respectively threaded to the upper bladder 1311 at the end away from the middle bladder 1312; the second sub-tube 122 near the first air collecting bladder 13 and the fourth sub-tube 222 near the second air collecting bladder 23 are respectively threaded to the lower bladder 1313 at the end away from the middle bladder 1312.

[0043] The diameter of the central capsule 1312 satisfies the following relationship:

[0044] and

[0045] In the formula, D D D represents the diameter of the central capsule 1312 when the diameter of the central capsule 1312 is used as the characteristic length. H Fr represents the diameter of the central cavity portion 1312, with the liquid level height H within the hollow cavity 131 as the characteristic length. DFr represents the Froude number calculated when the diameter of the middle capsule (1312 mm) is taken as the characteristic length. H The Froude number is calculated with the liquid level H inside the hollow cavity 131 as the characteristic length, g represents the acceleration due to gravity, and π represents the acceleration due to gravity. 2 It is the square of pi, v1 represents the fluid velocity of the first sub-pipe 121, the second sub-pipe 122, the third sub-pipe 221 and the fourth sub-pipe 222, and A1 represents the cross-sectional area of ​​the first sub-pipe 121, the second sub-pipe 122, the third sub-pipe 221 and the fourth sub-pipe 222.

[0046] Specifically, this portable microfluidic bubble manual removal device can also be a portable microfluidic bubble removal device, suitable for bubble removal in general microfluidic experiments, such as bubble removal for sealed fluid pipelines.

[0047] Continue to refer to Figure 2 As shown, the portable microfluidic bubble manual removal device includes a first venting component 1, a second venting component 2, a sample cell 3, and an ultrasonic bath 4. The first venting component 1 and the second venting component 2 are used for alternating venting, and the initial liquid injection must first enter through the second venting component 2. The sample cell 3 can be a quartz flow cell with an optical path of 10 mm. Depending on the actual observation requirements, other types of sample cells 3 can also be used, and no specific limitation is made for this embodiment.

[0048] The ultrasonic bath 4 is used for auxiliary exhaust and can be a KQ3200DB type CNC ultrasonic cleaner. This KQ3200DB type CNC ultrasonic cleaner has a volume of 3.2 liters, an ultrasonic frequency of 40kHz, a maximum power between 600W and 1200W, a heating power of approximately 1000W, and an inner tank material of stainless steel with dimensions of approximately 400mm × 250mm × 250mm. Of course, other models of ultrasonic bath 4 can be used depending on the actual situation; this embodiment does not impose specific limitations on this. The sample cell 3 is placed entirely within the ultrasonic bath 4.

[0049] Continue to refer to Figure 2As shown, the first exhaust component 1 includes a first syringe 11, a first tubing 12, and a first gas collecting bladder 13. The second exhaust component 2 includes a second syringe 21, a second tubing 22, and a second gas collecting bladder 23. The first syringe 11 and the second syringe 21 have the same structure and material. The first tubing 12 and the second tubing 22 also have the same structure and material. For example, both the first tubing 12 and the second tubing 22 are made of transparent composite materials, such as polyvinyl chloride thermoplastic elastomer, which has the strength, durability, and dimensional stability of polyvinyl chloride, while also possessing the flexibility, elasticity, and sealing properties of rubber. While achieving high transparency and easy observation, it maintains excellent weather resistance, chemical corrosion resistance, and tear resistance. The inner diameter of the first tubing 12 and the second tubing 22 are the same, for example, both the inner diameter of the first tubing 12 and the inner diameter of the second tubing 22 can be 1mm to 2mm. The first gas collecting bladder 13 and the second gas collecting bladder 23 have the same structure and material.

[0050] The syringe 110 is hollow inside and cylindrical on the outside. The wall thickness is uniform and the inner wall is smooth. The syringe 110 is not only a "container" for the solution, but also provides axial guidance for the movement of the push rod 111 and piston 112, ensuring that the piston 112 slides in a straight line and avoiding leakage caused by deflection.

[0051] The push rod 111 is not a single-diameter cylindrical rod, but a stepped structure with a diameter varying according to the functional segment. It includes a rod body, a piston connecting end, and a handle. The rod body is the main body of the push rod, with the smallest diameter, used to transmit thrust. The piston connecting end is a tapered / expanded structure at the end of the rod body, used to fix the piston 112. The handle is an expanded structure (such as a disc or fin) at the top of the push rod, with the largest diameter, facilitating the user to apply thrust or pull force. The "stepped cylindrical" design ensures the structural strength of the push rod 111 while achieving precise matching with the piston 112 and the syringe 110.

[0052] The piston 112 is made of an elastic material (such as rubber or silicone), and is in the shape of a ring or a disc. Its outer diameter is slightly larger than the inner diameter of the syringe, and it is sealed by interference fit.

[0053] The piston connecting end of the push rod 111 is fixedly connected to the piston 112 (e.g., by snap-fit ​​or adhesive bonding). The two are non-movable assemblies, ensuring that the push rod 111 can synchronously drive the piston 112 when it moves. The rod body of the push rod 111, the piston connecting end, and the piston 112 are all placed in the hollow cavity of the syringe 110. The piston 112 fits tightly against the inner wall of the syringe, forming a sealed structure to prevent the liquid from leaking out of the gap. The push rod's manual extension extends to the outside of the syringe 110, which is key to enabling manual operation.

[0054] The first pipeline 12 includes a first sub-pipeline 121 and a second sub-pipeline 122, which are connected by a first air-collecting bag 13. The second pipeline 22 includes a third sub-pipeline 221 and a fourth sub-pipeline 222, which are connected by a second air-collecting bag 23.

[0055] Appropriate amounts of solution are drawn into the syringe 110 of the first syringe 11 and the syringe 110 of the second syringe 21, respectively. Both the first syringe 11 and the second syringe 21 are vertically arranged in the position of syringe 110. Both syringe 110 of the first syringe 11 and the second syringe 21 are fixed. Optionally, both syringe 110 of the first syringe 11 and the second syringe 21 are fixed on the syringe holder 6. The first gas collecting bladder 13 and the second gas collecting bladder 23 are vertically arranged at both ends. The syringe 110, the first sub-tube 121, the first gas collecting bladder 13 and the second sub-tube 122 of the first syringe 11 are sequentially connected. The syringe 110, the third sub-tube 221 and the second gas collecting bladder 23 and the fourth sub-tube 222 of the second syringe 21 are sequentially connected. The second sub-tube 122 and the fourth sub-tube 222 are both connected to the sample cell 3. The end of the second sub-tube 122 away from the first gas collecting bladder 13 is inserted into the upper middle part of the solution in the sample cell 3, and the end of the fourth sub-tube 222 away from the second gas collecting bladder 23 is inserted into the lower middle part of the solution in the sample cell 3. The sample cell 3 is placed in the ultrasonic bath 4 to construct an integrated exhaust device, which simplifies the overall structure of the portable microfluidic bubble manual removal device and reduces additional requirements.

[0056] It should be noted that the end of the fourth sub-tube 222 furthest from the second gas collecting bladder 23 is inserted into the sample cell 3 near the bottom. By precisely controlling the extremely low inlet flow rate of the fourth sub-tube 222, the fluid is driven to gently wet and rise along the wall of the sample cell 3, forming a gradually rising meniscus. Capillary action is preferentially utilized to guide the liquid forward, allowing the gas in the sample cell 3 to be smoothly and directionally displaced and discharged, thereby reducing bubble formation at the source.

[0057] Combination Figure 2 , Figure 3 and Figure 4As shown, the first gas collecting bladder 13 and the second gas collecting bladder 23 are used to assist in the separation of gases in the solution. Both the first gas collecting bladder 13 and the second gas collecting bladder 23 include a hollow bladder cavity 131 and a shell 132. The shell 132 surrounds the hollow bladder cavity 131, and the hollow bladder cavity 131 and the shell 132 are an integral structure. The upper and lower parts of the hollow bladder cavity 131 are connected. The hollow bladder 131 includes an upper bladder portion 1311, a middle bladder portion 1312, and a lower bladder portion 1313 connected in sequence. The upper bladder portion 1311, the middle bladder portion 1312, and the lower bladder portion 1313 are all interconnected cavities. The upper bladder portion 1311 of the first gas collecting bladder 13 is closer to the first syringe 11, and the lower bladder portion 1313 of the first gas collecting bladder 13 is closer to the sample cell 3. The upper bladder portion 1311 of the second gas collecting bladder 23 is closer to the first syringe 11, and the lower bladder portion 1313 of the second gas collecting bladder 23 is closer to the sample cell 3. The upper bladder portion 1311, the middle bladder portion 1312, and the lower bladder portion 1313 of the first gas collecting bladder 13 and the second gas collecting bladder 23 are arranged vertically and parallel to the direction of gravity.

[0058] The upper bladder cavity 1311 and lower bladder cavity 1313 are semi-circular or conical in shape, while the middle bladder cavity 1312 is circular in shape. The upper bladder cavity 1311 and lower bladder cavity 1313 are replaced from the traditional "cylindrical structure" with a "semi-circular or conical structure." When fluid in the syringe 110 of the first syringe 11 flows from the first sub-tube 121 into the upper bladder cavity 1311 and the lower bladder cavity 1313 of the first air-collecting bladder 13 and then into the second sub-tube 122, or when fluid in the syringe 110 of the second syringe 21 flows from the second sub-tube 122... When the fluid flows into the upper cavity 1311 and lower cavity 1313 of the second air collecting bladder 23 and flows into the fourth sub-pipeline 222, the upper cavity 1311 of the first air collecting bladder 13 and the second air collecting bladder 23 provides a smooth transition of gradual expansion, while the lower cavity 1313 of the first air collecting bladder 13 and the second air collecting bladder 23 provides a smooth transition of gradual contraction. This eliminates the dead angle of the cylindrical right-angle edge, reduces the probability of bubble formation, and facilitates the rise and convergence of bubbles, avoiding the formation of local bubble accumulation areas. This embodiment only uses a semi-circular structure as an example.

[0059] The upper bladder cavity 1311, the middle bladder cavity 1312, and the lower bladder cavity 1313 are integrated into a single structure. This integrated structure not only eliminates the connection gaps and assembly tolerances between the upper bladder cavity 1311, the middle bladder cavity 1312, and the lower bladder cavity 1313, enabling it to withstand greater loads, vibrations, and impacts, but also avoids gaps between the upper bladder cavity 1311, the middle bladder cavity 1312, and the lower bladder cavity 1313, as well as between the shell 132 and the upper bladder cavity 1311, the middle bladder cavity 1312, and the lower bladder cavity 1313. There is a risk of air leakage at the connection between 1313. Compared to the separate first and second air-collecting bladders 23 in existing technology 1, this design avoids problems such as loose connections, deformation, and misalignment. It eliminates the need for fasteners, flanges, and corresponding assembly processes between the upper bladder cavity 1311, middle bladder cavity 1312, and lower bladder cavity 1313, as well as between the shell 132 and the upper, middle, and lower bladder bladder 1311, middle, and lower bladder cavity 1313, reducing component procurement costs and weight. It also shortens the production cycle and reduces the error rate of manual assembly.

[0060] The first sub-tube 121 of the first tube 12 is located between the end of the first air collecting bladder 13 near the first air collecting bladder 13 and the end of the upper bladder cavity 1311 of the first air collecting bladder 13 away from the middle bladder cavity 1312; the second sub-tube 122 of the first tube 12 is located between the end of the first air collecting bladder 13 near the first air collecting bladder 13 and the end of the lower bladder cavity 1313 of the first air collecting bladder 13 away from the middle bladder cavity 1312; the third sub-tube 221 of the second tube 22 is located between the end of the second air collecting bladder 23 near the upper bladder cavity 1311 of the second air collecting bladder 23 and the middle bladder cavity 1311 of the second air collecting bladder 23. The end away from the middle bladder cavity 1312 and the end of the fourth sub-tube 222 in the second tube 22 near the second air collecting bladder 23 are all connected by threads to the end of the lower bladder cavity 1313 in the second air collecting bladder 23 away from the middle bladder cavity 1312. The meshing surfaces of the threads can form a reliable seal. At the same time, with the mechanical locking effect of the threads, the first sub-tube 121, the second sub-tube 122, the third sub-tube 221 and the fourth sub-tube 222 can be prevented from falling off when the pressure is overloaded.

[0061] The diameter D of the central cavity 1312 and the liquid level H of the hollow cavity 131 in the first and second airbags 13 and 23 need to be adapted to the diameters of the first sub-tube 121, the second sub-tube 122, the third sub-tube 221, and the fourth sub-tube 222. In principle, the Froude number Fr should be less than or equal to 1. In the formula, v represents the flow velocity inside the hollow cavity 131 of the first air collecting bladder 13 and the second air collecting bladder 23, g represents the gravitational acceleration, and L represents the characteristic length, which can be the diameter of the hollow cavity 131 of the first air collecting bladder 13 and the second air collecting bladder 23 or the liquid level height of the hollow cavity 131 of the first air collecting bladder 13 and the second air collecting bladder 23.

[0062] If the characteristic length L is selected as the diameter D of the hollow cavity 131 of the first air-collecting bladder 131 and the second air-collecting bladder 23, and Fr << 1, then the diameter D of the hollow cavity 131 of the first air-collecting bladder 131 and the second air-collecting bladder 23 is... D The expression is:

[0063]

[0064] In the formula, D D Fr represents the diameter of the central capsule 1312 calculated using the diameter of the central capsule 1312 as the characteristic length. D The Froude number is calculated with the diameter of the central sac cavity (1312 mm) as the characteristic length, g represents the acceleration due to gravity, and π represents the acceleration due to gravity. 2 It is the square of pi. v1 represents the fluid velocity of the first sub-pipe 121, the second sub-pipe 122, the third sub-pipe 221, and the fourth sub-pipe 222, which can be understood as the fluid velocity of the first pipe 12 and the second pipe 22. A1 represents the cross-sectional area of ​​the first sub-pipe 121, the second sub-pipe 122, the third sub-pipe 221, and the fourth sub-pipe 222, which can be understood as the cross-sectional area of ​​the first pipe 12 and the second pipe 22.

[0065] If the characteristic length L is selected as the liquid level height H of the hollow cavity 131 of the first air collecting sac 13 and the second air collecting sac 23, and Fr << 1, then the diameter of the middle cavity portion 1312 is calculated using the liquid level height H inside the hollow cavity 131 as the characteristic length.

[0066] In the formula, D H Fr represents the diameter of the central cavity portion 1312 calculated with the liquid level height H inside the hollow cavity 131 as the characteristic length. H The Froude number is calculated with the liquid level H inside the hollow cavity 131 as the characteristic length, g represents the acceleration due to gravity, and π represents the acceleration due to gravity. 2 It is the square of pi. v1 represents the fluid velocity of the first sub-pipe 121, the second sub-pipe 122, the third sub-pipe 221, and the fourth sub-pipe 222, which can be understood as the fluid velocity of the first pipe 12 and the second pipe 22. A1 represents the cross-sectional area of ​​the first sub-pipe 121, the second sub-pipe 122, the third sub-pipe 221, and the fourth sub-pipe 222, which can be understood as the cross-sectional area of ​​the first pipe 12 and the second pipe 22.

[0067] In the structural design of the first airbag 13 and the second airbag 23, it is required that the above-mentioned D be satisfied simultaneously. D and D HThis ensures the exhaust effect of the first airbag 13 and the second airbag 23. Subsequently, the reciprocating push of the push rod 111 of the first syringe 11 and the push rod 111 of the second syringe 21, as well as the ultrasonic vibration of the ultrasonic bath 4, ensure that the air bubbles in the sealed fluid circuit can be effectively discharged.

[0068] Combination Figures 2 to 5 As shown, assuming Fr = 0.01 (satisfying Fr << 1), the diameters of the first sub-pipe 121, the second sub-pipe 122, the third sub-pipe 221, and the fourth sub-pipe 222 (such as the first pipe 12 and the second pipe 22) are 1.6 mm. The total internal height of the hollow cavities 131 in the first and second air collecting bladders 13 and 23 is 50 mm. The liquid level H in the hollow cavities 131 of the first and second air collecting bladders 13 and 23 is estimated as 90% of the total internal height of the hollow cavities 131. The peristaltic pump 8 in the sealed fluid circuit 7 and the first syringe 11 and the second syringe 21 are used to make the fluid flow in the first pipe 12 and the second pipe 22. The flow rate under different conditions is measured (the error is large when using manual flow). Substitute these values ​​into the above D. D and D H The formula was used for calculation, and the results are as follows:

[0069]

[0070] It should be noted that the relationship between the diameters of the cystic cavity 1312 is as follows: and It can also be simplified to the following relationship:

[0071] D1 / D2 < (gD2) 0.5 / v1 and D1 / D2 < (gH) 0.5 / v1,

[0072] In the formula, D1 represents the inner diameter of the first sub-pipe 121, the second sub-pipe 122, the third sub-pipe 221, and the fourth sub-pipe 222; D2 represents the inner diameter of the first air collecting bladder 13 and the second air collecting bladder 23; g represents the acceleration due to gravity; H represents the liquid level height in the hollow bladder 131 of the first air collecting bladder 13 and the second air collecting bladder 23; and v1 represents the fluid velocity of the first sub-pipe 121, the second sub-pipe 122, the third sub-pipe 221, and the fourth sub-pipe 222.

[0073] Continue to refer to Figures 2 to 5 As shown, the specific operation process of this portable microfluidic bubble manual removal device is as follows:

[0074] Step 1: Draw appropriate amounts of solution into the syringe 110 of the first syringe 11 and the syringe 110 of the second syringe 21 respectively. The syringe 110 of the first syringe 11 is connected to the sample cell 3 in sequence through the first sub-tube 121, the first gas collecting bladder 13, the second sub-tube 122, and the sample cell 3 in sequence. The syringe 110 of the second syringe 21 is connected to the sample cell 3 in sequence through the third sub-tube 221, the second gas collecting bladder 23, the fourth sub-tube 222, and the sample cell 3. The entire sample cell 3 is placed in the ultrasonic bath 4 to construct an integrated exhaust device.

[0075] Step 2: Fix the plunger 111 of the second syringe 21 and release the plunger 111 of the first syringe 11 so that it can move freely; slowly push the plunger 111 of the second syringe 21 so that the solution enters the sample cell 3 through the second gas collecting bladder 23. The inlet of the fourth sub-tube 222 is close to the bottom of the sample cell 3. Control the low flow rate so that the solution gently wets the cell wall of the sample cell 3 and the liquid level gradually rises, thereby reducing the generation of bubbles from the source.

[0076] Step three: The liquid flows out of the sample cell 3 from the second sub-tube 122, and then flows sequentially through the first gas collecting bladder 13 and the first sub-tube 121 into the syringe 110 of the first syringe 11 in the release state. Utilizing the density difference between gas and liquid and the principle of gravity separation, residual bubbles in the solution are automatically separated and collected in the first gas collecting bladder 13, preventing gas from re-entering the circulation and improving the thoroughness of venting. Specifically, based on the inherent density difference between the gas and liquid phases, the vertically placed first gas collecting bladder 13 can achieve a spontaneous gas-liquid separation process by utilizing the gravitational field. This is because: on the one hand, the buoyancy of the bubbles is much greater than their own weight; on the other hand, the flow cross-sectional size of the first gas collecting bladder 13 is larger than the cross-sectional size of the first sub-tube 121 and the second sub-tube 122. When the gas-liquid mixture flows into the first gas collecting bladder 13 from the second sub-tube 122, the flow velocity drops sharply, and the fluid (including gas and liquid) can no longer transport bubbles. The force governing the fluid behavior changes from inertial force to gravity and buoyancy, creating conditions for gravity separation. Meanwhile, the denser liquid phase settles naturally under gravity, eventually forming a stable gas phase region in the upper cavity 1311 (top) of the first gas collecting bladder 13, while the lower cavity 1313 (bottom) forms a liquid phase region. Furthermore, because the gas-liquid interface within the first gas collecting bladder 13 is far from the openings of the first sub-pipe 121 and the second sub-pipe 122, it experiences less disturbance from newly flowing fluid, which is beneficial for stable gas-liquid separation. The diameter of the middle cavity 1312 of the hollow cavity 131 in the first gas collecting bladder 13 and the liquid level need to be adapted to the fluid pipeline diameter to satisfy the Froude number Fr << 1. This includes the following two design requirements: First, the Froude number Fr calculated based on the diameter of the middle cavity 1312 as the characteristic length. D Using Froude number Fr DThe diameter D of the central capsule cavity 1312 was calculated. D The diameter D of the cystic cavity 1312 D First, it is used to fully suppress fluid inertial forces, so that the flow behavior becomes dominated by gravity; second, it is based on the Froude number Fr calculated when the liquid level height H inside the hollow cavity 131 is taken as the characteristic length. H The Froude number Fr H The diameter D of the central capsule cavity 1312 was calculated. H The diameter D of the cystic cavity 1312 H This ensures that the bubbles have sufficient time to float to the surface due to density differences within the liquid inside the capsule, thus achieving gas-liquid separation.

[0077] Step four: If a small number of bubbles remain in sample cell 3, turn on the power and ultrasonic waves of ultrasonic bath 4. High-frequency mechanical vibration (e.g., using a KQ3200DB CNC ultrasonic cleaner, the ultrasonic working frequency is approximately 40kHz, meaning the mechanical vibration frequency of the internal vibrator is 40,000 times / second) breaks down and drives the bubbles. Simultaneously, the second syringe 21 continues to inject liquid, utilizing the synergistic effect of flow shear and transport to enhance bubble removal, significantly shortening processing time and reducing energy consumption. Specifically, the high-frequency ultrasonic degassing mechanism is as follows: When high-intensity ultrasonic waves propagate in a liquid, alternating compressive and rarefied phases are generated. In the rarefied phase, liquid molecules are stretched, forming tiny vacuum cavities or causing existing tiny bubbles in the solution to rapidly expand, generating cavitation bubbles. These cavitation bubbles collapse at extremely high speeds in the subsequent compressive phase, generating enormous instantaneous local stress, destroying the bubbles and their attachment points, causing the bubbles to detach from the container wall.

[0078] The mechanism by which flow shear causes bubbles to detach is as follows: Bubbles attached to the wall of the third sub-pipe 221 are in a state of equilibrium under the influence of various forces such as surface tension and buoyancy. Flow shear disrupts this mechanical equilibrium. When the fluid flows within the third sub-pipe 221, due to the viscosity of the fluid, a boundary layer with a velocity gradient forms near the wall. The shear stress generated by the velocity gradient causes the bubbles to be simultaneously subjected to a drag force pulling them away from their attachment point and a torque that causes the bubbles to deform and roll. When the velocity gradient is large enough, the bubbles deform and become unstable, beginning to slide or roll away from the wall.

[0079] Fluid flow transport carries away air bubbles: Once a bubble detaches from the wall, it is captured by the fluid flow, changing from a fixed state to a floating state suspended in the fluid, and thus carried by the flow into the second air collection bladder 23 instead of reattaching elsewhere. Therefore, the two stages of flow shearing desorption and fluid flow transport constitute an efficient and continuous exhaust chain. The continuous flow maintains the necessary wall shearing to detach the bubbles, while also acting as a transport medium to continuously transport the detached bubbles to the second air collection bladder 23. This tandem mechanism of shearing desorption and flow transport constitutes a purely physical exhaust scheme that does not require an external complex medium, which not only improves exhaust efficiency but also significantly reduces system complexity and processing costs.

[0080] Step five: For adhering air bubbles that are difficult to remove from the vessel walls or corners, the direction of fluid flow is switched by alternately fixing and releasing the first syringe 11 and the second syringe 21, thereby inducing fluid oscillation. This operation can induce unsteady flow and local turbulence, enhancing the bubble removal effect. It achieves efficient venting without the need for a complex external drive mechanism, balancing low system cost and high reliability.

[0081] Step six: If the above steps still do not completely remove all air bubbles, all solution in the portable microfluidic bubble manual removal device can be reinjected into the second syringe 21, and steps two through five can be repeated. This cyclical operation process has high repeatability and fault tolerance, avoiding sample waste and rework costs caused by incomplete venting, and improving overall operating efficiency.

[0082] Steps one through six above are active operations, which require sequentially completing the following steps: solution extraction, liquid injection and venting, ultrasonic assistance, fluid oscillation, and repeated optimization. The first syringe 11 and the second syringe 21 are manually controlled to alternate actions, which is an "active" method for removing air bubbles from the sealed fluid circuit.

[0083] It should be noted that the second sub-tube 122 and the fourth sub-tube 222 are inserted into the sample cell 3 on both sides in the direction from the second sub-tube 122 to the fourth sub-tube 222. The top surface of the sample cell 3 can be an inclined surface, and the side where the second sub-tube 122 is inserted is at a lower position, while the side where the fourth sub-tube 222 is inserted is at a higher position, which further enhances the self-expulsion capability of bubbles and effectively improves the filling efficiency.

[0084] The technical features described in the following are not conventional in this field: "The first syringe 11, the first tubing 12, and the first gas collecting bladder 13 are placed vertically; the second exhaust component 2 includes a second syringe 21, a second tubing 22, and a second gas collecting bladder 23, which are also placed vertically; both the first gas collecting bladder 13 and the second gas collecting bladder 23 include a hollow bladder cavity 131 and a cylindrical shell 132 surrounding the hollow solution portion; the hollow bladder cavity 131 includes an upper bladder cavity 1311, a middle bladder cavity 1312, and a lower bladder cavity 1313 connected sequentially; the upper bladder cavity 1311 and the lower bladder cavity 1313 are semi-circular or conical in shape, the middle bladder cavity 1312 is circular in shape, and the upper bladder cavity 1311, the middle bladder cavity 1312, and the lower bladder cavity 1313 are an integral structure."

[0085] First, in prior art 1, the first airbag 2' and the second airbag 4' are separate units, which need to be reassembled before use; prior art 2 does not use an airbag design.

[0086] Secondly, under normal circumstances, if there is gas in the pipeline of a sealed fluid circuit, those skilled in the art typically use the following two methods to handle it: Method 1, to make the fluid in the pipeline of the sealed fluid circuit flow or accelerate the flow to carry away the air bubbles. However, if the air bubbles are adsorbed on the inner wall or in a weak flow area, flow alone cannot remove them, especially in some special cases where it is inconvenient to apply flow (such as in narrow operating spaces, or when the sample solution is particularly expensive and cannot be prepared in large quantities); Method 2, it is necessary to completely empty the solution and refill it, controlling the flow rate during refilling. The alternating driving of the first syringe 11 and the second syringe 21, the repeated changes in the fluid flow direction to oscillate the fluid for exhaust, and the spontaneous gas-liquid separation and gas collection under gravity in the first gas collecting bladder 13 and the second gas collecting bladder 23 used in this embodiment have the advantages of simple operation, strong adaptability, small sample requirement (focusing on observing microfluidics, such as the existence state, distribution position and removal effect of air bubbles), and can be used in sealed fluid circuits (no pump drive required and no reliance on external mother liquor for solution exchange).

[0087] The diameter of the central capsule cavity 1312 satisfies the following relationship: and "This is also not a conventional design in this field, as detailed below."

[0088] Prior art 1 does not address the matching relationship between the diameters of the first air-collecting bladder 2' and the second air-collecting bladder 4' and the pipeline, and prior art 2 also does not disclose the limitation of the diameter of the bladder cavity 1312 in this embodiment. This embodiment ensures the discharge of air bubbles from the sealed fluid pipeline by limiting the diameter of the bladder cavity 1312.

[0089] Compared with the prior art, the portable microfluidic bubble manual removal device provided in this embodiment achieves at least the following beneficial effects:

[0090] First, in the structural design of the first airbag 13 and the second airbag 23, it is required that the diameter D of the middle airbag cavity 1312, obtained when the diameter of the middle airbag cavity 1312 is used as the characteristic length, be simultaneously satisfied. D The diameter D of the central cyst portion 1312, obtained when the liquid level height H inside the hollow cyst 131 is a characteristic length. H This ensures the effective venting of the first air-collecting bladder 13 and the second air-collecting bladder 23, and can also be used in sealed fluid pipelines of different specifications. Through the synergistic interaction between the first venting component 1, the second venting component 2, the sample cell 3, and the ultrasonic bath 4, not only can the air bubbles in the sealed fluid circuit be effectively vented, but also the air bubbles on the walls of each component of the fluid circuit can be eliminated. This solves the problem of not being able to ensure that the air bubbles in the micro-sealed pipeline can be effectively vented and the air bubbles on the walls of each component of the fluid circuit can be eliminated.

[0091] Secondly, compared to the prior art 1 which uses an electric injection structure to drive the syringe and separate first and second air-collecting bladders 2' and 4', this embodiment eliminates the filter membrane or mesh of the first and second air-collecting bladders 13 and 23, as well as the electric injection structure used to fix and push the syringe. In this embodiment, the first and second air-collecting bladders 13 and 23 are integrated structures. The upper and lower ends of the first air-collecting bladder 13 are directly assembled with the first sub-tube 121 and the second sub-tube 122, respectively, and the upper and lower ends of the second air-collecting bladder 23 are assembled with the third sub-tube 221 and the fourth sub-tube 222, respectively. Compared to the prior art 1, this embodiment has fewer parts, making it easier to replace parts (e.g., no need to replace the filter, mesh, electric injection structure, etc.). Moreover, it occupies less space overall and can also be applied to sealed fluid circuits. This solves the problem in the prior art 1 where the first air-collecting bladder 2', the second air-collecting bladder 4', and the electric injection structure involve many parts, and once damaged, the disassembly and replacement of faulty parts requires breaking through multiple assembly steps, resulting in inconvenient parts replacement.

[0092] Third, the upper bladder cavity 1311, the middle bladder cavity 1312, and the lower bladder cavity 1313 are integrated into a single structure. This integrated structure not only eliminates the connection gaps and assembly tolerances between the upper bladder cavity 1311, the middle bladder cavity 1312, and the lower bladder cavity 1313, enabling it to withstand greater loads, vibrations, and impacts, but also avoids gaps between the upper bladder cavity 1311, the middle bladder cavity 1312, and the lower bladder cavity 1313, as well as between the shell 132 and the upper bladder cavity 1311, the middle bladder cavity 1312, and the lower bladder cavity. There is a risk of air leakage at the connection between parts 1313. Compared to the separate first and second air-collecting bladders 13 and 23 in the prior art 1, this design avoids problems such as loose connections, deformation, and misalignment. It eliminates the need for fasteners, flanges, and corresponding assembly processes between the upper bladder cavity 1311, middle bladder cavity 1312, and lower bladder cavity 1313, as well as between the shell 132 and the upper, middle, and lower bladder cavity 1313, reducing component procurement costs and weight. It also shortens the production cycle and reduces the error rate of manual assembly.

[0093] Fourth, the upper bladder cavity 1311 and the lower bladder cavity 1313 are semi-circular or conical in shape, while the middle bladder cavity 1312 is circular in shape. Replacing the traditional "cylindrical structure" of the upper bladder cavity 1311 and the lower bladder cavity 1313 with a "semi-circular or conical structure" allows for fluid flow from the first sub-tube 121 into the upper bladder cavity 1311 and the lower bladder cavity 1313 of the first air-collecting bladder 13, and then into the second sub-tube 122. Alternatively, when fluid in the second syringe 21 flows from the second sub-tube... When the fluid flows from the upper cavity 1311 and lower cavity 1313 of the second air collecting bladder 23 into the fourth sub-pipe 222 via the first air collecting bladder 13 and the second air collecting bladder 23, the upper cavity 1311 provides a smooth transition for the fluid to gradually expand, while the lower cavity 1313 provides a smooth transition for the fluid to gradually contract. This eliminates the dead angles at the right-angled edges of the cylinder, reduces the probability of bubble formation, and facilitates the rise and convergence of bubbles, avoiding the formation of local bubble accumulation areas.

[0094] Fifth, compared to the prior art 1 which uses an electric injection structure to drive the syringe and separate first and second air collection bags 2' and 4', in this embodiment, the upper bag cavity 1311, the middle bag cavity 1312 and the lower bag cavity 1313, as well as the shell 132 and the upper bag cavity 1311, the middle bag cavity 1312 and the lower bag cavity 1313 are all integrated structures. Furthermore, the electric injection structure in the prior art 1 is eliminated, which significantly reduces the price cost and the time cost of assembling various components.

[0095] In one alternative embodiment, combined with Figure 2 and Figure 6 As shown, Figure 6 This is a schematic diagram of the structure of the three-way valve provided by the present invention; both the second sub-pipeline 122 and the fourth sub-pipeline 222 include a first sub-pipe section 1221 and a second sub-pipe section 1222 connected to the first sub-pipe section 1221. The length extension direction of the first sub-pipe section 1221 is perpendicular to the length extension direction of a portion of the second sub-pipe section 1222. The side of the first sub-pipe section 1221 away from the second sub-pipe section 1222 is threadedly connected to the end of the lower bladder section 1313 away from the middle bladder section 1312. The side of the second sub-pipe section 1222 away from the first sub-pipe section 1221 is inserted into the upper middle part of the solution in the sample cell 3. The side of the second sub-pipe section 1222 away from the first sub-pipe section 1221 is inserted into the lower middle part of the solution in the sample cell 3.

[0096] The side of the first sub-pipe section 1221 near the second sub-pipe section 1222 is connected to the side of the second sub-pipe section 1222 near the first sub-pipe section 1221 via a three-way valve 5.

[0097] Specifically, continue to refer to Figure 2 and Figure 6 As shown, both the second sub-channel 122 and the fourth sub-channel 222 include a first sub-channel portion 1221 and a second sub-channel portion 1222. The first sub-channel portion 1221 is straight, and the second sub-channel portion 1222 is L-shaped. The right angle of the second sub-channel portion 1222 faces the sample cell 3. The length extension direction of the first sub-channel portion 1221 in the second sub-channel 122 is the same as the length extension direction of the first sub-channel 121. The length extension direction of the first sub-tube 1221 in the second sub-tube 122 is perpendicular to the length extension direction of the other part of the second sub-tube 1222. The second sub-tube 1222 parallel to the length extension direction of the first sub-tube 1221 in the second sub-tube 122 is inserted into the upper middle part of the solution in the sample cell 3. Preferably, the second sub-tube 1222 parallel to the first sub-tube 1221 in the second sub-tube 122 is inserted into the top of the solution in the sample cell 3.

[0098] The length extension direction of the first sub-tube 1221 in the fourth sub-tube 222 is the same as that of the third sub-tube 221. The length extension direction of the first sub-tube 1221 in the fourth sub-tube 222 is perpendicular to the length extension direction of a portion of the second sub-tube 1222. The length extension direction of the first sub-tube 1221 in the fourth sub-tube 222 is parallel to the length extension direction of another portion of the second sub-tube 1222. The second sub-tube 1222 parallel to the first sub-tube 1221 in the fourth sub-tube 222 is inserted into the lower middle part of the solution in the sample cell 3. Preferably, the second sub-tube 1222 parallel to the first sub-tube 1221 in the second sub-tube 1222 is inserted into the bottom of the solution in the sample cell 3.

[0099] The side of the first sub-pipe section 1221 near the second sub-pipe section 1222 is connected to the side of the second sub-pipe section 1222 near the first sub-pipe section 1221 via a three-way valve 5. The three-way valve 5 includes a three-way cavity 51, a valve core, and a knob. The three-way cavity 51 has three ports: the first port is connected to the first sub-pipe section 1221, the second port is connected to the second sub-pipe section 1222, and the third port is connected to the sealed fluid circuit 7. The interiors of the three ports form a T-shaped intersection. The sealing valve core 52 is located at the center of the intersection inside the three-way cavity 51 and is the core component for switching the flow path. The knob is located outside the three-way cavity 51 and is coaxially connected to the sealing valve core 52. By rotating the knob (the rotation angle can be 0°, 90°, 180°, or 360°), the position of the valve core can be controlled to achieve the switching of the flow path.

[0100] The second sub-pipe 122 in the first pipe 12 and the fourth sub-pipe 222 in the second pipe 22 are respectively connected to the sealed fluid circuit 7 via a three-way valve 5. When the sample is pretreated in the sealed fluid circuit 7, it is treated using the sealed fluid circuit 7. After pretreatment, the sealed fluid circuit 7 needs to be vented. This is done by switching to a portable microfluidic bubble manual venting device via the three-way valve 5. After venting, experimental observations are performed. Specifically, when pretreatment of the sealed fluid circuit 7 is required, the knob is rotated to connect the first sub-pipe 1221, the second sub-pipe 1222, and the sealed fluid circuit 7. The body circuits 7 are not interconnected. When it is necessary to vent the sealed fluid pipeline, rotate the knob to connect the first sub-pipe 1221, the second sub-pipe 1222 and the sealed fluid circuit 7. The gas in the sealed fluid circuit 7 enters the first sub-pipe 1221 and the second sub-pipe 1222. Then rotate the knob to connect the first sub-pipe 1221 and the second sub-pipe 1222. The first sub-pipe 1221 and the second sub-pipe 1222 are disconnected from the sealed fluid circuit 7, and the sealed fluid circuit 7 is vented. After venting, the sealed fluid circuit 7 is experimentally observed.

[0101] It should be noted that the pretreatment circuit of the above sample in the sealed fluid circuit 7 can refer to the research content of Xu, S.; Zhou, H.; Sun, Z.; Xie, J., Formation of an fcc phase through a bcc metastable state incrystallization of charged colloidal particles. Phys. Rev. E 2010, 82, 010401 [This paper, published by Xu et al. in Physical Review E in 2010, entitled "Formation of a face-centered cubic phase through a body-centered cubic metastable state during the crystallization of charged colloidal particles"]. This embodiment does not make specific limitations on this.

[0102] By adopting the above scheme, not only can the venting of the sealed fluid circuit 7 be achieved, but the pretreatment of the sealed fluid circuit 7 can also be achieved, and the two do not interfere with each other.

[0103] In one optional embodiment, the upper bladder cavity 1311 is provided with a first internal threaded hole 13110 on the side away from the middle bladder cavity 1312, and the first sub-tube 121 is provided with a first external threaded interface matching the first internal threaded hole 13110 on the side of the first air collecting bladder 13 and the third sub-tube 221 is provided with a first external threaded interface matching the first internal threaded hole 13110 on the side of the third sub-tube 221 near the second air collecting bladder 23. The first external threaded interface is threadedly connected to the first internal threaded hole 13110.

[0104] The lower bladder cavity 1313 has a second internal threaded hole 13130 ​​on the side away from the middle bladder cavity 1312. The second sub-tube 122 is provided with a second external threaded interface that matches the second internal threaded hole 13130 ​​on the side near the first air collecting bladder 13 and the fourth sub-tube 222 is provided with a second external threaded interface that matches the second internal threaded hole 13130. The second external threaded interface is threadedly connected to the second internal threaded hole 13130.

[0105] Specifically, continue to refer to Figure 3 and Figure 4 As shown, a first internal threaded hole 13110 is provided on the side of the upper cavity 1311 of the hollow cavity 131 in the first air collecting bladder 13 and the second air collecting bladder 23 away from the middle cavity 1312. The first internal threaded hole 13110 penetrates the shell 132 and the upper cavity 1311 in the direction from the upper cavity 1311 to the lower cavity 1313. The first internal threaded hole 13110 extends in the direction from the upper cavity 1311 to the lower cavity 1313. A first external threaded interface is provided on the side of the first sub-tube 121 near the first air collecting bladder 13 and the side of the third sub-tube 221 near the second air collecting bladder 23. The first external threaded interface matches the first internal threaded hole 13110 and is screwed into the first internal threaded hole 13110.

[0106] A second internally threaded hole 13130 ​​is provided on the side of the lower cavity 1313 of the hollow cavity 131 in the first airbag 13 and the second airbag 23 away from the middle cavity 1312. The second internally threaded hole 13130 ​​penetrates the shell 132 and the lower cavity 1313 in a direction from the upper cavity 1311 to the lower cavity 1313.

[0107] The second sub-pipeline 122 is provided with a second external threaded interface on the side near the first air collecting bag 13 and the fourth sub-pipeline 222 is provided with a second external threaded interface on the side near the second air collecting bag 23. The second external threaded interface matches the second internal threaded hole 13130 ​​and is screwed into the second internal threaded hole 13130.

[0108] In practical use, the first sub-tube 121 is connected to the upper cavity 1311 of the hollow cavity 131 in the first air collecting bag 13, and the third sub-tube 221 is connected to the upper cavity 1311 of the hollow cavity 131 in the second air collecting bag 23 through the first external threaded interface and the first internal threaded hole 13110; the second sub-tube 122 is connected to the lower cavity 1313 of the hollow cavity 131 in the first air collecting bag 13, and the fourth sub-tube 222 is connected to the lower cavity 1313 of the hollow cavity 131 in the second air collecting bag 23 through the second external threaded interface and the second internal threaded hole 13130.

[0109] By adopting the above solution, on the one hand, the axial clamping force generated by the threaded engagement achieves a reliable seal between the first external thread interface and the second external thread interface, effectively preventing leakage of the microfluidic fluid during the exhaust process; on the other hand, its inherent mechanical locking structure can withstand the internal pressure fluctuations of the portable microfluidic bubble manual removal device, fundamentally avoiding the risk of accidental detachment between the first sub-tube 121 and the first air collecting bladder 13, between the first air collecting bladder 13 and the second sub-tube 122, between the third sub-tube 221 and the second air collecting bladder 23, and between the second air collecting bladder 23 and the fourth sub-tube 222 due to excessive pressure.

[0110] In one alternative embodiment, continue to refer to Figure 2 As shown, the syringe barrel 110 of the first syringe 11 is threadedly connected to the first sub-tube 121 near the syringe barrel 11; the syringe barrel 110 of the second syringe 21 is threadedly connected to the third sub-tube 221 near the syringe barrel 21. Specifically, both the syringe barrel 110 of the first syringe 11 near the first sub-tube 121 and the syringe barrel 110 of the second syringe 21 near the third sub-tube 221 are provided with a third internal threaded hole, which extends in the direction from the upper bladder portion 1311 to the lower bladder portion 1313; the first sub-tube 121 near the syringe barrel 110 of the first syringe 11 and the third sub-tube 221 near the syringe barrel 110 of the second syringe 21 are provided with a third external threaded interface, which matches the third internal threaded hole and is screwed into the third internal threaded hole.

[0111] By adopting the above solution, on the one hand, the axial clamping force generated by the thread engagement achieves a reliable seal of the third external thread interface, effectively preventing leakage of the microfluidic fluid during the exhaust process; on the other hand, its inherent mechanical locking structure can withstand the internal pressure fluctuations of the portable microfluidic bubble manual removal device, fundamentally avoiding the risk of accidental detachment between the first syringe 11 and the first sub-tube 121, and between the second syringe 21 and the third sub-tube 221 due to excessive pressure.

[0112] In one alternative embodiment, continue to refer to Figure 2 and Figure 7 As shown, Figure 7 This is a schematic diagram of the structure of the first or second syringe provided by the present invention. In this embodiment, the syringe 110 includes a hollow cylindrical body 1101 and a conical head 1102 connected to the body 1101. The push rod 111 is axially movable and accommodated in the body 1101. The conical head 1102 is conical in shape and is located at one end of the body 1101 along its length. In the first syringe 11, the conical head 1102 is threadedly connected to the first sub-tube 121 near the side of the first syringe 11. In the second syringe 21, the conical head 1102 is threadedly connected to the third sub-tube 221 near the side of the second syringe 21.

[0113] Specifically, continue to refer to Figure 2 and Figure 7 As shown, the first syringe 11 and the second syringe 21 have the same structure, both including a syringe barrel 110 and a push rod 111 adapted to and connected to the syringe barrel 110. The syringe barrel 110 is used to contain a solution. The syringe barrel 110 includes a barrel body 1101 and a cone head 1102 disposed at one end of the barrel body 1101. The barrel body 1101 and the cone head 1102 are coaxially arranged. The internal channel of the cone head 1102 communicates with the hollow cavity of the barrel body 1101. The push rod 111 is movably housed inside the barrel body 1101 along the axial direction of the barrel body 1101. A piston 112 is fixedly assembled at its piston connection end. The piston 112 is tightly fitted with the inner wall of the barrel body 1101 to form a sealing structure. The push rod 111 drives the piston 112 to reciprocate linearly along the axial direction of the barrel body to assist in venting.

[0114] Both the cone 1102 of the first syringe 11 and the cone 1102 of the second syringe 21 are provided with a third internal thread hole. The third internal thread hole extends in the direction from the upper bladder portion 1311 to the lower bladder portion 1313. The first sub-tube 121 near the first syringe 11 and the third sub-tube 221 near the second syringe 21 are provided with a third external thread interface. The third external thread interface matches the third internal thread hole and is screwed into the third internal thread hole.

[0115] The above solution not only has a simple overall structure and low price, but also achieves a reliable seal for the third external thread interface, effectively preventing leakage of the microfluidic fluid during the exhaust process. At the same time, it fundamentally avoids the risk of accidental detachment between the cone 1102 of the first syringe 11 and the first sub-tube 121, and between the cone 1102 of the second syringe 21 and the third sub-tube 221 due to excessive pressure.

[0116] In one alternative embodiment, continue to refer to Figure 2 , Figure 7 and Figure 8 As shown, Figure 8 This is a schematic diagram of the structure of the syringe holder provided by the present invention; the syringes 110 in the first syringe 11 and the second syringe 21 are respectively fixed on the syringe holder 6;

[0117] The syringe holder 6 includes a hollow cylindrical clamping member 61, a long strip-shaped connecting member 62, and a rectangular conical fixing member 63. The clamping member 61 is connected to the conical fixing member 63 through the connecting member 62. The clamping member 61, the connecting member 62, and the conical fixing member 63 are an integral structure. The length extension direction of the connecting member 62 is perpendicular to the length extension direction of the conical fixing member 63. The clamping member 61 is used to clamp the upper middle part of the syringe 110.

[0118] The cone head fixing member 63 has a cone head fixing hole 631 that matches the cone head 1102. The cone head fixing hole 631 penetrates the cone head fixing member 63 along its thickness direction, and the cone head 1102 is inserted into the cone head fixing hole 631.

[0119] Specifically, continue to refer to Figure 2 , Figure 7 and Figure 8 As shown, the syringes 110 in the first syringe 11 and the second syringe 21 are respectively fixed on the syringe holder 6, and the syringe holder 6 is placed in a vertical position.

[0120] The syringe holder 6 includes a hollow cylindrical clamping member 61, a long strip-shaped connector 62, and a rectangular conical fixing member 63. The clamping member 61, the connector 62, and the conical fixing member 63 are integrally formed. The connector 62 is located between the clamping member 61 and the conical fixing member 63. The length extension direction of the connector 62 is the same as the length extension direction of the syringe 110 of the first syringe 11 or the second syringe 21, and the length extension direction of the connector 62 is perpendicular to the length extension direction of the conical fixing member 63. The clamping member 61 is used to clamp the upper middle part of the syringe 110.

[0121] The cone head fixing member 63 has a cone head fixing hole 631 that matches the cone head 1102. The cone head fixing hole 631 passes through the cone head fixing member 63 along the length direction of the syringe 110. The cone head fixing hole 631 matches the cone head 1102, and the cone head 1102 passes through the cone head fixing hole 631.

[0122] The aforementioned clamping member 61 includes a semi-circular first clamping part 611 and a second clamping part 612, and the semi-circular first clamping part 611 and the second clamping part 612 form a hollow cylindrical structure, and the syringe 110 is placed inside the hollow cylindrical structure.

[0123] The first clamping part 611 and the second clamping part 612 each include a fixed end and a free end. The fixed end of the first clamping part 611 and the fixed end of the second clamping part 612 are integrally connected. A rectangular locking block 6110 is connected to the free end of the first clamping part 611 and the second clamping part 612 away from the connector 62. The locking block 6110 has a locking hole. The locking hole passes through the locking block 6110 in the direction from the first clamping part 611 to the second clamping part 612. The locking block 6110 in the first clamping part 611 and the locking block 6110 in the second clamping part 612 are connected together by a locking bolt 64 and a locking hole. By rotating the locking bolt 64, the clamping force between the first clamping part 611 and the second clamping part 612 can be adjusted.

[0124] The connector 62 is used to connect to the surface of an object. The connector 62 has at least four connecting holes 621, which are arranged in a cross or matrix. The connecting holes 621 penetrate the connector 62 along its thickness direction. Of course, the number of connecting holes can be increased or decreased according to the actual situation. This embodiment does not make a specific limitation on this. This embodiment uses 7 connecting holes 621 as an example.

[0125] By adopting the above solution, the syringe 110 of the first syringe 11 and the second syringe 21 can be effectively fixed, so that the operator can push the push rod 111 of the first syringe 11 or the second syringe 21.

[0126] In one optional embodiment, both the first gas collecting bladder 13 and the second gas collecting bladder 23 are made of transparent material. Using transparent material to fabricate the first gas collecting bladder 13 and the second gas collecting bladder 23 enables in-situ visual monitoring of the gas-liquid separation process and bubble discharge, facilitating observation of the flow field state and separation efficiency. Optionally, the transparent material includes plexiglass or polypropylene. Plexiglass has high light transmittance (approximately 92%) and good surface hardness, making it suitable for precision observation. Polypropylene is transparent, low in cost, and resistant to chemical corrosion, making it suitable for specific fluid environments.

[0127] As can be seen from the above embodiments, the portable microfluidic bubble manual removal device provided by the present invention achieves at least the following beneficial effects:

[0128] First, in the structural design of the first and second gas collecting bladders, it is required that the diameter of the central bladder cavity, obtained when the diameter of the central bladder cavity is used as the characteristic length, and the diameter of the central bladder cavity, obtained when the liquid level height inside the hollow bladder cavity is used as the characteristic length, are simultaneously satisfied. This ensures the venting effect of the first and second gas collecting bladders and also allows them to be used in sealed fluid pipelines of different specifications. Through the synergy between the first venting component, the second venting component, the sample cell, and the ultrasonic bath, it is possible not only to ensure that air bubbles in the sealed fluid circuit can be effectively vented, but also to remove air bubbles from the walls of each component in the fluid circuit. This solves the problem of not being able to ensure that air bubbles in the micro-sealed pipeline can be effectively vented and to remove air bubbles from the walls of each component in the fluid circuit.

[0129] Secondly, compared to the prior art 1 which uses an electric injection structure to drive the syringe and separate first and second air-collecting bladders, the present invention eliminates the filter membrane or mesh of the first and second air-collecting bladders, as well as the electric injection structure used to fix and push the syringe. In this embodiment, the first and second air-collecting bladders are integrated into one unit. The upper and lower ends of the first air-collecting bladder are directly assembled with the first and second sub-tubules, respectively, and the upper and lower ends of the second air-collecting bladder are assembled with the third and fourth sub-tubules, respectively. Compared to the prior art 1, this embodiment has fewer parts, making it easier to replace parts (e.g., no need to replace the filter, mesh, electric injection structure, etc.). Moreover, it occupies less space overall and can also be applied to sealed fluid circuits. This solves the problem in the prior art 1 where the first air-collecting bladder, second air-collecting bladder, and electric injection structure involve many parts, and once damaged, the disassembly and replacement of faulty parts requires breaking through multiple assembly steps, resulting in inconvenient parts replacement.

[0130] Third, the upper, middle, and lower air chambers are integrated into a single structure. This integrated structure not only eliminates connection gaps and assembly tolerances between the upper, middle, and lower air chambers, allowing them to withstand greater loads, vibrations, and impacts, but also avoids the risk of air leakage at the joints between the upper, middle, and lower air chambers, as well as between the shell and the upper, middle, and lower air chambers. Furthermore, compared to the separate first and second air chambers in existing technology 1, it avoids problems such as loose connections, deformation, and misalignment. It eliminates the need for fasteners, flanges, and corresponding assembly processes between the upper, middle, and lower air chambers, as well as between the shell and the upper, middle, and lower air chambers, reducing component procurement costs and weight. It also shortens the production cycle and reduces the error rate of manual assembly.

[0131] Fourth, the upper and lower bladder cavities are semi-circular or conical in shape, while the middle bladder cavity is circular. By replacing the traditional "cylindrical structure" with a "semi-circular or conical structure," when the fluid in the first syringe flows from the first sub-tube into the upper and lower bladder cavities of the first gas collecting bladder and then into the second sub-tube, or when the fluid in the second syringe flows from the second sub-tube into the upper and lower bladder cavities of the second gas collecting bladder and then into the fourth sub-tube, the upper bladder cavities of the first and second gas collecting bladders provide a smooth, gradually expanding transition for the fluid, while the lower bladder cavities provide a smooth, gradually contracting transition. This eliminates the dead angles of the cylindrical right-angled edges, reduces the probability of bubble formation, and facilitates the rise and convergence of bubbles, avoiding the formation of localized bubble accumulation areas.

[0132] While specific embodiments of the invention have been described in detail by way of examples, those skilled in the art should understand that the examples are for illustrative purposes only and not intended to limit the scope of the invention. Those skilled in the art should understand that modifications can be made to the above embodiments without departing from the scope and spirit of the invention. The scope of the invention is defined by the appended claims.

Claims

1. A portable microfluidic bubble manual removal device, characterized in that, It includes a first exhaust component, a second exhaust component, a sample cell, and an ultrasonic bath. The first exhaust component and the second exhaust component are arranged in a mirror-symmetrical manner. The sample cell contains a solution and is disposed inside the ultrasonic bath. The first exhaust device includes a first syringe, a first tubing, and a first gas collecting bladder. The first syringe and the first gas collecting bladder are placed vertically. The first tubing includes a first sub-tubing and a second sub-tubing that are connected. The second exhaust device includes a second syringe, a second tubing, and a second gas collecting bladder. The second syringe and the second gas collecting bladder are placed vertically. The second tubing includes a third sub-tubing and a fourth sub-tubing that are connected. Both the first syringe and the second syringe include a hollow cylindrical syringe and a plunger that matches the syringe. The plunger is a stepped cylindrical rod structure. The plunger is sealed to the inner wall of the syringe through a piston. The rod body and piston connection end of the plunger are located inside the syringe. The pusher extension of the plunger extends to the outside of the syringe. The plunger drives the piston to slide along the axis of the syringe. The syringe barrel of the first syringe is connected to the end of the first gas collecting bladder near the first syringe via a first sub-tube, the end of the first gas collecting bladder away from the first syringe is connected to a second sub-tube, and the end of the second sub-tube away from the first gas collecting bladder is inserted into the upper middle part of the solution in the sample cell; the syringe barrel of the second syringe is connected to the end of the second gas collecting bladder near the second syringe via a third sub-tube, the end of the second gas collecting bladder away from the second syringe is connected to a fourth sub-tube, and the end of the fourth sub-tube away from the second gas collecting bladder is inserted into the lower middle part of the solution in the sample cell; Both the first and second air-collecting bladders include a hollow bladder cavity and a cylindrical shell surrounding the hollow solution portion. The hollow bladder cavity includes an upper bladder cavity, a middle bladder cavity, and a lower bladder cavity connected in sequence. The upper and lower bladder cavities are semi-circular or conical in shape, and the middle bladder cavity is circular in shape. The upper, middle, and lower bladder cavities are an integral structure. The first sub-tube near the first air-collecting bladder and the third sub-tube near the second air-collecting bladder are respectively threaded to the upper bladder cavity at the end away from the middle bladder cavity. The second sub-tube near the first air-collecting bladder and the fourth sub-tube near the second air-collecting bladder are respectively threaded to the lower bladder cavity at the end away from the middle bladder cavity. The diameter of the central cystic cavity satisfies the following relationship: and In the formula, D D D represents the diameter of the central cyst cavity, obtained when the diameter of the central cyst cavity is used as the characteristic length. H Fr represents the diameter of the central cavity portion, calculated with the liquid level height H within the hollow cavity as the characteristic length. D Fr represents the Froude number calculated when the diameter of the central cystic portion is used as the characteristic length. H The Froude number is calculated with the liquid level height H inside the hollow cavity as the characteristic length, g represents the acceleration due to gravity, and π represents the acceleration due to gravity. 2 It is the square of pi, v1 represents the fluid velocity of the first, second, third and fourth sub-pipes, and A1 represents the cross-sectional area of ​​the first, second, third and fourth sub-pipes.

2. The portable microfluidic bubble manual removal device according to claim 1, characterized in that, Both the second and fourth sub-channels include a first sub-channel and a second sub-channel connected to the first sub-channel. The length extension direction of the first sub-channel is perpendicular to the length extension direction of a portion of the second sub-channel. The side of the first sub-channel away from the second sub-channel is threadedly connected to the end of the lower sac cavity away from the middle sac cavity. In the second sub-channel, the side of the second sub-channel away from the first sub-channel is inserted into the upper middle part of the solution in the sample cell. In the fourth sub-channel, the side of the second sub-channel away from the first sub-channel is inserted into the lower middle part of the solution in the sample cell. The side of the first sub-tube section closest to the second sub-tube section is connected to the side of the second sub-tube section closest to the first sub-tube section via a three-way valve.

3. The portable microfluidic bubble manual removal device according to claim 1, characterized in that, The upper bladder cavity is provided with a first internal threaded hole on the side away from the middle bladder cavity. The first sub-pipeline is provided with a first external threaded interface matching the first internal threaded hole on the side near the first air collection bladder and the third sub-pipeline is provided with a first external threaded interface on the side near the second air collection bladder. The first external threaded interface is threadedly connected to the first internal threaded hole. The lower bladder cavity is provided with a second internal threaded hole on the side away from the middle bladder cavity. The second sub-pipeline is provided with a second external threaded interface that matches the second internal threaded hole on the side near the first air collection bladder and the fourth sub-pipeline is provided with a second external threaded interface on the side near the second air collection bladder. The second external threaded interface is threadedly connected to the second internal threaded hole.

4. The portable microfluidic bubble manual removal device according to claim 1, characterized in that, The syringe barrel of the first syringe is threadedly connected to the side of the first sub-tube near the syringe barrel of the first syringe; the syringe barrel of the second syringe is threadedly connected to the side of the third sub-tube near the syringe barrel of the second syringe.

5. The portable microfluidic bubble manual removal device according to claim 4, characterized in that, The syringe includes a hollow cylindrical body and a conical head connected to the body. The push rod is axially movable and accommodated within the body. The conical head is conical in shape and is located at one end of the body along its length. In the first syringe, the conical head is threadedly connected to the first sub-tube on the side near the first syringe. In the second syringe, the conical head is threadedly connected to the third sub-tube on the side near the second syringe.

6. The portable microfluidic bubble manual removal device according to claim 1, characterized in that, The syringes in the first syringe and the second syringe are respectively fixed on syringe holders; The syringe holder includes a hollow cylindrical clamping member, a long strip-shaped connecting member, and a rectangular conical fixing member. The clamping member is connected to the conical fixing member through the connecting member. The clamping member, the connecting member, and the conical fixing member are an integral structure. The length extension direction of the connecting member is perpendicular to the length extension direction of the conical fixing member. The clamping member is used to clamp the upper middle part of the syringe. The cone head fixing member has a cone head fixing hole that matches the cone head. The cone head fixing hole penetrates the cone head fixing member along its thickness direction, and the cone head is inserted into the cone head fixing hole.

7. The portable microfluidic bubble manual removal device according to claim 1, characterized in that, Both the first and second airbags are made of transparent material.

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