Microfluidic nucleic acid detector and detection method thereof

Abstract

The invention discloses a microfluidic nucleic acid detector and a detection method thereof in the technical field of nucleic acid detection.The microfluidic nucleic acid detector comprises a shell, a material suction rod is slidably connected to the top end of the shell, a pressing block is arranged on one side of the top end of the shell, an outer tube is slidably connected to the interior of the shell, an inner tube is arranged in the outer tube, the inner tube is fixedly connected with the shell, and the material suction rod is slidably connected to the top end of the shell. The bottom end of the inner pipe penetrates through the outer pipe and is arranged below the outer pipe, the bottom of the inner pipe is sleeved with a suction nozzle, and an auxiliary replacement assembly is arranged on the shell. Through the adoption of the auxiliary replacement assembly, the used suction nozzle is automatically taken down from the bottom of the inner pipe to be replaced after single-time suction and use are completed; in the prior art, accurate single-time butt joint is difficult during manual butt joint, and in order to guarantee the sterile effect, a suction nozzle cannot be held by a hand to be in butt joint with an inner tube, so that on the premise of a large cardinal number of nucleic acid detection reagents, the suction nozzle is automatically replaced through an auxiliary replacement assembly, and a lot of time can be saved for detection results.

Description

Technical Field

[0001] This invention relates to the field of nucleic acid detection technology, specifically to a microfluidic nucleic acid detector and its detection method. Background Technology

[0002] Nucleic acid testing is used to detect the presence of invading viral nucleic acid in respiratory specimens, blood, or feces from patients to determine whether they are infected with the virus. Therefore, a positive nucleic acid test proves that the virus is present in the patient's body.

[0003] When determining target cells in collected viral samples for nucleic acid testing, a pipette is needed to add the reaction solution and virus into the same test tube for reaction. Before use, a pipette tip needs to be installed at the bottom, and the tip needs to be replaced after each contact with the liquid. Since there are a large number of samples for nucleic acid testing, and the required testing time should be minimized, the frequent replacement of pipette tips will have a significant impact on the efficiency of nucleic acid testing.

[0004] Based on this, the present invention designs a microfluidic nucleic acid detector and its detection method to solve the problem of insufficient efficiency caused by frequent manual replacement of pipette tips in nucleic acid detection. Summary of the Invention

[0005] The purpose of this invention is to provide a microfluidic nucleic acid detector and its detection method to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a microfluidic nucleic acid detector, comprising a housing, a suction rod slidably connected to the top of the housing, a pressing block provided on one side of the top of the housing, an outer tube slidably connected inside the housing, an inner tube provided inside the outer tube, the inner tube being fixedly connected to the housing, the bottom end of the inner tube passing through the outer tube and positioned below the outer tube, a suction nozzle sleeved on the bottom of the inner tube, and an auxiliary replacement component provided on the housing, the auxiliary replacement component being used to remove the used suction nozzle from the bottom of the inner tube and replace it with a new suction nozzle for re-sleeving on the inner tube for use.

[0007] As a further embodiment of the present invention, the auxiliary replacement component includes a connecting rope, one end of which is fixedly connected to the inner tube. A sliding rod is slidably connected inside the bottom end of the first gear. A second spring for resetting the sliding rod is provided inside the first gear. A groove is provided on the side wall of the first gear. One end of the connecting rope passes through the groove and is fixedly connected to the sliding rod. A fixing plate is fixedly connected to the bottom end of the first gear. A clamping claw is slidably connected to one end of the fixing plate. A third spring for resetting the clamping claw is provided on the clamping claw. A cone block is fixedly connected to the bottom end of the sliding rod. A conical groove for engaging with the cone block is provided on the top of the clamping claw. A baffle is fixedly connected to one side of the bottom end of the first gear. A threaded groove is provided on the outer wall of the inner tube.

[0008] As a further embodiment of the present invention, a first gear is rotatably connected to the outer tube, and a mating block that mates with the threaded groove is fixedly connected inside the first gear. A threaded telescopic rod is fixedly connected to the bottom of the housing, and a second gear that meshes with the first gear is fixedly connected to the threaded telescopic rod. A fixed frame is rotatably connected to the output end of the threaded telescopic rod, and a stop bar is rotatably connected to each of the two side walls of the fixed frame. A torsion spring for resetting the stop bar is provided on the stop bar, and a toggle block for moving the stop bar is fixedly connected to the side wall of the first gear.

[0009] As a further embodiment of the present invention, a storage frame is provided at the bottom of the housing, a connecting rod is fixedly connected to the top of the storage frame, a protrusion for docking with the housing is slidably connected to the top of the connecting rod, a sliding rod is fixedly connected inside the storage frame, a base plate is slidably connected to one end of the sliding rod, a first spring for resetting is fixedly connected to one end of the base plate, a push rod is fixedly connected to the bottom end of the base plate, and the bottom end of the push rod passes through the storage frame and is located below the storage frame.

[0010] As a further embodiment of the present invention, an alignment plate is fixedly connected to the top of the fixed frame, and the alignment plate is used to restrict the sliding of the base plate on the slide rod.

[0011] As a further embodiment of the present invention, the inner wall of the slide bar can fit against the outer wall of the suction nozzle, and an acrylic plate is provided on the side wall of the storage frame.

[0012] As a further embodiment of the present invention, the outer wall of the outer tube can be completely fitted with the inner wall of the inner tube, and the outer wall of the inner tube is a smooth wall.

[0013] A microfluidic nucleic acid detection method, comprising the following steps:

[0014] Step 1: Install the pipette tip to the bottom of the inner tube, insert the pipette tip into the nucleic acid test reagent beaker and draw aspirate according to the set value;

[0015] Step 2: Move the housing to the nozzle recycling and storage area. Use the auxiliary replacement component to detach the used nozzle from the inner tube and place it in the storage area. The new nozzle will be automatically installed at the bottom of the inner tube.

[0016] Step 3: Continue to draw the reagent into the nozzle for testing by pressing;

[0017] Step 4: After the test is completed, remove the suction nozzle from the bottom of the inner tube.

[0018] Compared with the prior art, the beneficial effects of the present invention are:

[0019] 1. This invention employs an auxiliary replacement component that automatically removes and replaces the used pipette tip from the bottom of the inner tube after each use, saving time spent manually replacing the tip after each use. This is because the tip has a small diameter, making it difficult to accurately connect manually in one go. Furthermore, to ensure sterility, the tip cannot be held by hand while connecting to the inner tube. Thus, given the large quantity of nucleic acid testing reagents, the automatic replacement of the tip using the auxiliary replacement component can save a significant amount of time in obtaining test results. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0021] Figure 2 for Figure 1 Enlarged structural diagram at point A in the middle;

[0022] Figure 3 This is a schematic diagram of the internal structure of the storage frame;

[0023] Figure 4 This is a schematic diagram of a partial cross-sectional structure of the outer tube;

[0024] Figure 5 for Figure 4 Enlarged structural diagram at point B;

[0025] Figure 6 This is a schematic diagram of the inner tube and the first gear structure;

[0026] Figure 7 Schematic diagram of threaded telescopic rod and fixing frame structure;

[0027] Figure 8 This is a flowchart of the method of the present invention.

[0028] The attached diagram lists the components represented by each number as follows:

[0029] 1. Pressing block; 2. Suction rod; 3. Housing; 4. Suction nozzle; 5. Storage frame; 6. Alignment plate; 7. Threaded telescopic rod; 8. Outer tube; 9. First gear; 10. Second gear; 11. Fixing frame; 12. Pulley; 13. Connecting rope; 14. Slide groove; 15. Inner tube; 16. Baffle; 17. First spring; 18. Push rod; 19. Base plate; 20. Slide rod; 21. Connecting rod; 22. Protrusion; 23. Second spring; 24. Sliding rod; 25. Conical block; 26. Fixing plate; 27. Third spring; 28. Gripper; 29. ​​Threaded groove; 30. Connecting block; 31. Stop bar. Detailed Implementation

[0030] Please see Figures 1-8 This invention provides a technical solution: a microfluidic nucleic acid detector, comprising a housing 3, a suction rod 2 slidably connected to the top of the housing 3, a pressing block 1 provided on one side of the top of the housing 3, an outer tube 8 slidably connected inside the housing 3, an inner tube 15 provided inside the outer tube 8, the inner tube 15 being fixedly connected to the housing 3, the bottom end of the inner tube 15 passing through the outer tube 8 and positioned below the outer tube 8, a suction nozzle 4 sleeved on the bottom of the inner tube 15, and an auxiliary replacement component provided on the housing 3, the auxiliary replacement component being used to remove the used suction nozzle 4 from the bottom of the inner tube 15 and replace it with a new suction nozzle 4 for re-sleeving on the inner tube 15 for use;

[0031] When the above solution is put into practical use, the pipette 4 is installed at the bottom of the inner tube 15. The pipette 4 is inserted into the nucleic acid test reagent beaker and aspirated according to the set value. The shell 3 is moved to the pipette 4 recycling and storage area. The used pipette 4 is detached from the inner tube 15 and placed in the storage area by the auxiliary replacement component, and a new pipette 4 is automatically installed at the bottom of the inner tube 15. The reagent is then drawn into the pipette 4 by pressing the suction rod 2 for testing. After the test is completed, the pipette 4 at the bottom of the inner tube 15 can be removed. The purpose of this is to automatically remove and replace the used pipette 4 from the bottom of the inner tube 15 after each use, which can save the time of manually replacing the pipette 4 after each use. Because the diameter of the pipette 4 is small, it is difficult to accurately connect it manually in one go. Moreover, in order to ensure the sterility effect, the pipette 4 cannot be held by hand to connect with the inner tube 15. Thus, under the premise of a large number of nucleic acid test reagents, the automatic replacement of the pipette 4 by the auxiliary replacement component can save a lot of time for the test results.

[0032] As a further embodiment of the present invention, the auxiliary replacement component includes a connecting rope 13, one end of which is fixedly connected to the inner tube 15. A sliding rod 24 is slidably connected inside the bottom end of the first gear 9. A second spring 23 for resetting the sliding rod 24 is provided inside the first gear 9. A groove 14 is provided on the side wall of the first gear 9. One end of the connecting rope 13 passes through the groove 14 and is fixedly connected to the sliding rod 24. A fixing plate 26 is fixedly connected to the bottom end of the first gear 9. A clamp 28 is slidably connected to one end of the fixing plate 26. A third spring 27 for resetting the clamp 28 is provided on the clamp 28. A cone block 25 is fixedly connected to the bottom end of the sliding rod 24. A conical groove for engaging with the cone block 25 is provided on the top of the clamp 28. A baffle 16 is fixedly connected to one side of the bottom end of the first gear 9. A threaded groove 29 is provided on the outer wall of the inner tube 15.

[0033] A first gear 9 is rotatably connected to the outer tube 8. A mating block 30 that mates with the threaded groove 29 is fixedly connected inside the first gear 9. A threaded telescopic rod 7 is fixedly connected to the bottom of the housing 3. A second gear 10 that meshes with the first gear 9 is fixedly connected to the threaded telescopic rod 7. A fixed frame 11 is rotatably connected to the output end of the threaded telescopic rod 7. A stop bar 31 is rotatably connected to each of the two side walls of the fixed frame 11. A torsion spring for resetting is provided on the stop bar 31. A toggle block 12 for moving the stop bar 31 is fixedly connected to the side wall of the first gear 9.

[0034] When the above solution is put into actual use, when replacing the suction nozzle 4, the housing 3 is moved to the top of the collection box for collecting the suction nozzle 4, and the pressing block 1 is pressed down. The pressing block 1 drives the outer tube 8 to press down, and the suction nozzle 4 is disengaged from the bottom of the inner tube 15 by the gripper 28 at the bottom of the outer tube 8. During the downward movement of the outer tube 8, the connecting rope 13 is gradually straightened. When the connecting rope 13 is completely straightened, the outer tube 8 continues to slide down. The connecting rope 13 will drive the sliding rod 24 to compress the second spring 23 and slide relative to the outer tube 8, so that the cone 25 at the bottom of the sliding rod 24 is disengaged from the top of the gripper 28. After being freed from the restriction of the cone 25, the third spring 27 will drive the gripper 28 to slide, so that the gripping effect of the gripper 28 on the suction nozzle 4 is released, and the suction nozzle 4 is disengaged from the bottom of the gripper 28. At this time, the collection action of the used suction nozzle 4 is completed.

[0035] As the outer tube 8 slides down, it drives the first gear 9 to slide down as well. This causes the mating block 30 inside the first gear 9 to rotate along the threaded groove 29 as the first gear 9 slides down. The rotation of the first gear 9 drives the second gear 10 to rotate as well. The rotation of the second gear 10 causes the output shaft inside the threaded telescopic rod 7 to extend downward, thereby causing the fixed frame 11 to slide downward. The downward speed of the fixed frame 11 is faster than that of the outer tube 8. When the suction nozzle 4 is about to detach from the bottom of the inner tube 15, the fixed frame 11 has already slid to the bottom of the inner tube 15. After the suction nozzle 4 detaches from the bottom of the inner tube 15, releasing the pressing block 1 causes the first gear 9 to rise. The toggle block 12 on the side wall of the outer tube 8 will contact the stop bar 31 and actuate the stop bar 31. Then, the entire housing 3 is tilted at a certain angle. After it is released from the limit of the stop bar 31, the suction nozzle 4 will slide along the fixed frame 11 to the bottom of the inner tube 15 under the action of gravity. Since the entire outer tube 8 has begun to move upward at this time, the opened gripper 28 will also slowly return to its original position. During the reset process, the suction nozzle 4 inside the fixed frame 11 enters the two grippers 28 and is prevented from falling further by the two grippers 28 and the baffle 16. As the outer tube 8 moves upward, the two grippers 28 gradually clamp and fix the suction nozzle 4 onto the inner tube 15, thus completing the replacement of the suction nozzle 4. During this process, the suction nozzle 4 will only fall off when the grippers 28 are fully open. At this time, directly installing the suction nozzle 4 will not connect it to the grippers 28 and it will fall off. When replacing the suction nozzle 4 during the clamping process of the grippers 28, the suction nozzle 4 can be hung on the grippers 28 but will not be clamped tightly. With the limiting of the baffle 16, the suction nozzle 4 will not fall off the grippers 28. As the grippers 28 clamp, the suction nozzle 4 can be fixed. The advantage of this is that after removing the used suction nozzle 4, a new suction nozzle 4 can be immediately installed at the bottom of the inner tube 15 for continued use, saving the time of manually replacing and connecting the suction nozzle 4, and effectively improving the detection efficiency.

[0036] As a further embodiment of the present invention, a storage frame 5 is provided at the bottom of the housing 3, a connecting rod 21 is fixedly connected to the top of the storage frame 5, a protrusion 22 for docking with the housing 3 is slidably connected to the top of the connecting rod 21, a sliding rod 20 is fixedly connected inside the storage frame 5, a base plate 19 is slidably connected to one end of the sliding rod 20, a first spring 17 for resetting is fixedly connected to one end of the base plate 19, a push rod 18 is fixedly connected to the bottom end of the base plate 19, the bottom end of the push rod 18 passes through the storage frame 5 and is located below the storage frame 5, and an alignment plate 6 is fixedly connected to the top of the fixed frame 11, the alignment plate 6 is used to restrict the sliding of the base plate 19 on the sliding rod 20;

[0037] When the above solution is put into actual use, the unused suction nozzle 4 is installed inside the storage frame 5. The storage frame 5 is fixed to the housing 3 by connecting rod 21. The outlet end of the storage frame 5 is aligned with the fixed frame 11. The storage frame 5 functions like a magazine. When the fixed frame 11 slides down to replenish the suction nozzle 4 inside to the bottom of the inner tube 15, when the fixed frame 11 slides up and resets, the suction nozzle 4 inside the storage frame 5 can be transferred to the inside of the fixed frame 11 under the elastic force of the first spring 17. Due to the restriction of the alignment plate 6 at the top of the fixed frame 11, when the fixed frame 11 slides down from one side of the storage frame 5, the alignment plate 6 can still block the first spring 17 from continuing to push the suction nozzle 4 from one end of the storage frame 5. Only after the suction nozzle 4 is reset will the first spring 17 continue to push the suction nozzle 4 into the inside of the fixed frame 11.

[0038] As a further embodiment of the present invention, the inner wall of the slide bar 20 can fit against the outer wall of the suction nozzle 4, and an acrylic plate is provided on the side wall of the storage frame 5.

[0039] When the above solution is put into actual use, the slide bar 20, when fully fitted together, can effectively limit the suction nozzle 4 inside the storage frame 5, preventing the suction nozzle 4 from tipping over inside the storage frame 5. The number of remaining suction nozzles 4 inside the storage frame 5 can be observed through the acrylic plate on the storage frame 5, making it easy to replenish them at any time.

[0040] As a further embodiment of the present invention, the outer wall of the outer tube 8 can be completely fitted with the inner wall of the inner tube 15, and the outer wall of the inner tube 15 is a smooth wall.

[0041] When the above solution is put into actual use, the two parts fit together perfectly, resulting in less friction when the outer tube 8 slides outside the inner tube 15, and less wear between the outer tube 8 and the inner tube 15.

[0042] A microfluidic nucleic acid detection method, comprising the following steps:

[0043] Step 1: Install the pipette tip 4 to the bottom of the inner tube 15, insert the pipette tip 4 into the nucleic acid test reagent beaker and draw aspirate according to the set value;

[0044] Step 2: Move the housing 3 to the nozzle 4 recycling and storage area. Use the auxiliary replacement component to detach the used nozzle 4 from the inner tube 15 to the storage area, and automatically install the new nozzle 4 to the bottom of the inner tube 15.

[0045] Step 3: Continue to draw the reagent into the nozzle 4 by pressing 2 for testing;

[0046] Step 4: After the test is completed, remove the suction nozzle 4 from the bottom of the inner tube 15.

[0047] Working principle: Install the nozzle 4 to the bottom of the inner tube 15, insert the nozzle 4 into the nucleic acid test reagent beaker and draw aspirate according to the set value. When replacing the nozzle 4, move the shell 3 to the top of the recycling box for recycling the nozzle 4, press down the pressing block 1, and the pressing block 1 will drive the outer tube 8 to press down. The clamp 28 at the bottom of the outer tube 8 will drive the nozzle 4 to detach from the bottom of the inner tube 15. As the outer tube 8 slides down, the connecting rope 13 is gradually straightened. When the connecting rope 13 is completely straightened, the outer tube 8 continues to slide down. The connecting rope 13 will drive the sliding rod 24 to compress the second spring 23 and slide relative to the outer tube 8, so that the cone 25 at the bottom of the sliding rod 24 will detach from the top of the clamp 28. After being freed from the restriction of the cone 25, the third spring 27 will drive the clamp 28 to slide, so that the clamping effect of the clamp 28 on the nozzle 4 is released, and the nozzle 4 will detach from the bottom of the clamp 28. At this time, the recycling action of the used nozzle 4 is completed.

[0048] As the outer tube 8 slides down, it drives the first gear 9 to slide down as well. This causes the mating block 30 inside the first gear 9 to rotate along the threaded groove 29 as the first gear 9 slides down. The rotation of the first gear 9 drives the second gear 10 to rotate as well. The rotation of the second gear 10 causes the output shaft inside the threaded telescopic rod 7 to extend downward, thereby causing the fixed frame 11 to slide downward. The downward speed of the fixed frame 11 is faster than that of the outer tube 8. When the suction nozzle 4 is about to detach from the bottom of the inner tube 15, the fixed frame 11 has already slid to the bottom of the inner tube 15. After the suction nozzle 4 detaches from the bottom of the inner tube 15, releasing the pressing block 1 causes the first gear 9 to rise. The toggle block 12 on the side wall of the outer tube 8 will contact the stop bar 31 and actuate the stop bar 31. Then, the entire housing 3 is tilted at a certain angle. After it is released from the limit of the stop bar 31, the suction nozzle 4 will slide along the fixed frame 11 to the inner tube 15 under the action of gravity. At the bottom, as the outer tube 8 begins to move upward, the open grippers 28 will slowly return to their original position. During the return of the grippers 28, the nozzle 4 inside the fixed frame 11 enters the two grippers 28 and is prevented from falling further by the two grippers 28 and the baffle 16. As the outer tube 8 moves upward, the two grippers 28 gradually clamp and fix the nozzle 4 to the inner tube 15, thus completing the replacement of the nozzle 4. During this process, the nozzle 4 will only fall off when the grippers 28 are fully open. At this time, directly installing the nozzle 4 will not connect it to the grippers 28 and it will fall off. When replacing the nozzle 4 during the return of the grippers 28, the nozzle 4 can be hung on the grippers 28 but will not be clamped tightly. With the limit of the baffle 16, the nozzle 4 will not fall off the grippers 28, and the clamping of the grippers 28 will fix the nozzle 4.

Claims

1. A microfluidic nucleic acid detector, comprising a housing (3), wherein a suction rod (2) is slidably connected to the top of the housing (3), a pressing block (1) is provided on one side of the top of the housing (3), an outer tube (8) is slidably connected inside the housing (3), an inner tube (15) is provided inside the outer tube (8), the inner tube (15) is fixedly connected to the housing (3), the bottom end of the inner tube (15) passes through the outer tube (8) and is located below the outer tube (8), and a suction nozzle (4) is sleeved on the bottom of the inner tube (15), characterized in that: The housing (3) is provided with an auxiliary replacement component, which is used to remove the used nozzle (4) from the bottom of the inner tube (15) and replace it with a new nozzle (4) and reattach it to the inner tube (15) for use.

2. The microfluidic nucleic acid detection instrument according to claim 1, characterized in that: The auxiliary replacement component includes a connecting rope (13), one end of which is fixedly connected to the inner tube (15). A sliding rod (24) is slidably connected inside the bottom end of the first gear (9). A second spring (23) for resetting the sliding rod (24) is provided inside the first gear (9). A groove (14) is provided on the side wall of the first gear (9). One end of the connecting rope (13) passes through the groove (14) and is fixedly connected to the sliding rod (24). 9) A fixing plate (26) is fixedly connected to the bottom end. A gripper (28) is slidably connected to one end of the fixing plate (26). A third spring (27) for resetting is provided on the gripper (28). A cone block (25) is fixedly connected to the bottom end of the sliding rod (24). A conical groove for docking with the cone block (25) is opened on the top of the gripper (28). A baffle (16) is fixedly connected to one side of the bottom end of the first gear (9). A threaded groove (29) is opened on the outer wall of the inner tube (15).

3. A microfluidic nucleic acid detection instrument according to claim 2, characterized in that: A first gear (9) is rotatably connected to the outer tube (8). A mating block (30) that mates with the threaded groove (29) is fixedly connected inside the first gear (9). A threaded telescopic rod (7) is fixedly connected to the bottom of the housing (3). A second gear (10) that meshes with the first gear (9) is fixedly connected to the threaded telescopic rod (7). A fixed frame (11) is rotatably connected to the output end of the threaded telescopic rod (7). A stop bar (31) is rotatably connected to each of the two side walls of the fixed frame (11). A torsion spring for resetting the stop bar (31) is provided on the stop bar (31). A paddle block (12) for moving the stop bar (31) is fixedly connected to the side wall of the first gear (9).

4. A microfluidic nucleic acid detection instrument according to claim 3, characterized in that: The bottom of the housing (3) is provided with a storage frame (5), and the top of the storage frame (5) is fixedly connected with a connecting rod (21). The top of the connecting rod (21) is slidably connected with a protrusion (22) for docking with the housing (3). The storage frame (5) is fixedly connected with a slide rod (20). One end of the slide rod (20) is slidably connected with a base plate (19). One end of the base plate (19) is fixedly connected with a first spring (17) for its reset. The bottom end of the base plate (19) is fixedly connected with a push rod (18). The bottom end of the push rod (18) passes through the storage frame (5) and is located below the storage frame (5).

5. A microfluidic nucleic acid detection instrument according to claim 4, characterized in that: The top of the fixed frame (11) is fixedly connected to an alignment plate (6), which is used to restrict the sliding of the bottom plate (19) on the slide rod (20).

6. A microfluidic nucleic acid detection instrument according to claim 4, characterized in that: The inner wall of the slide bar (20) can fit against the outer wall of the suction nozzle (4), and an acrylic plate is provided on the side wall of the storage frame (5).

7. A microfluidic nucleic acid detection instrument according to claim 2, characterized in that: The outer wall of the outer tube (8) can be completely fitted with the inner wall of the inner tube (15), and the outer wall of the inner tube (15) is a smooth wall.

8. A microfluidic nucleic acid detection method, applicable to any of the microfluidic nucleic acid detection instruments described in claims 1-7, characterized in that, The method includes the following steps: Step 1: Install the pipette tip (4) to the bottom of the inner tube (15), insert the pipette tip (4) into the nucleic acid test reagent beaker and draw up the sample according to the set value; Step 2: Move the housing (3) to the nozzle (4) recycling and storage area. Use the auxiliary replacement component to remove the used nozzle (4) from the inner tube (15) to the storage area, and automatically install the new nozzle (4) to the bottom of the inner tube (15). Step 3: Continue to draw the reagent into the nozzle (4) by pressing (2) for testing; Step 4: After the test is completed, remove the suction nozzle (4) at the bottom of the inner tube (15).

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