A soothing and repairing composition and its application in cosmetics

Abstract

This invention discloses a soothing and repairing composition and its application in cosmetics, belonging to the field of cosmetic technology. The soothing and repairing composition contains water lily polysaccharide, fibroin, and Paris polyphylla extract; the mass ratio of water lily polysaccharide, fibroin, and Paris polyphylla extract is (50~250):(100~500):(10~100). The above-mentioned soothing and repairing composition can synergistically inhibit the overexpression of key inflammatory factors (such as NO, IL-6, TNF-α) through multiple mechanisms, while promoting the repair of the skin barrier, thereby achieving excellent soothing and repairing effects with low irritation, and is particularly suitable for sensitive skin and acne-prone skin. Cosmetics containing the above-mentioned soothing and repairing composition can also achieve good soothing and repairing effects and are also suitable for sensitive skin and acne-prone skin.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and more specifically, to a soothing and repairing composition and its application in cosmetics. Background Technology

[0002] With increasing external environmental pressures and changing personal care habits, skin sensitivity, redness, stinging, and weakened skin barrier function are becoming increasingly prominent among consumers. Market research indicates that the demand for cosmetics with soothing and repairing functions continues to grow. Consumers not only focus on the immediate soothing effect of products but also value the gentleness and safety of the formula and its long-term suitability for sensitive, acne-prone, and other delicate skin types.

[0003] Currently, most mainstream soothing skincare products on the market rely on classic ingredients such as dipotassium glycyrrhizate, bisabolol, or centella asiatica extract. These ingredients have been proven in cosmetic applications to have certain anti-irritant and redness-relieving effects; however, their effects are often relatively singular: for example, dipotassium glycyrrhizate primarily relieves burning and itching; bisabolol helps reduce immediate discomfort caused by external stimuli; and centella asiatica extract focuses on supporting the skin's repair process. In actual formulations, these ingredients are often used alone or in simple combinations, making it difficult to achieve multi-dimensional and synergistic soothing and repair in complex skin stress states.

[0004] Furthermore, many existing products fail to achieve a good balance between relieving skin discomfort and repairing the skin barrier. While some products can alleviate irritation in the short term, they offer limited assistance in restoring the skin's own barrier function; others focus on physical repair but are insufficient in regulating inflammatory factors. For sensitive skin with multiple issues such as redness, fragility, and dryness, or acne-prone skin in an inflamed state, traditional single-ingredient or simple compound systems often appear to have insufficient overall efficacy, resulting in slow improvement. Moreover, due to issues with ingredient concentration or compatibility, they may cause potential discomfort to some highly sensitive individuals.

[0005] Therefore, the cosmetics industry still needs to develop a new type of soothing and repairing composition that should have the following characteristics: it can alleviate skin discomfort through the synergy of multiple active ingredients and multiple pathways; while soothing, it can effectively help restore the skin barrier function; and the overall formula is gentle and suitable for long-term use on a variety of fragile skin types, including sensitive skin and acne-prone skin.

[0006] In view of this, the present invention is proposed. Summary of the Invention

[0007] The purpose of this invention is to provide a soothing and repairing composition and its application in cosmetics, so as to solve or improve the above-mentioned technical problems.

[0008] This invention can be implemented as follows:

[0009] In a first aspect, the present invention provides a soothing and repairing composition containing water lily polysaccharide, silk protein and Paris polyphylla extract;

[0010] The mass ratio of water lily polysaccharide, silk protein and Paris polyphylla extract is (50~250):(100~500):(10~100).

[0011] In an optional embodiment, the mass ratio of water lily polysaccharide, fibroin and Paris polyphylla extract is (200~250):(100~300):(80~100).

[0012] In an optional embodiment, the mass ratio of water lily polysaccharide, silk protein, and Paris polyphylla extract is 200:300:80.

[0013] In an optional embodiment, the content of Paris saponin I in the Paris extract is ≥500 ppm.

[0014] Secondly, the present invention provides a cosmetic product containing the soothing and repairing composition of any of the foregoing embodiments.

[0015] In an optional implementation, the cosmetic includes a serum.

[0016] In an optional embodiment, the soothing and repairing composition is present in the cosmetic at a concentration of 0.5 wt% to 1.5 wt%.

[0017] In optional embodiments, the cosmetic also includes moisturizers, emulsifiers, skin-warming agents, thickeners, chelating agents, and antioxidants.

[0018] In an optional embodiment, the cosmetic contains 4 wt% to 5 wt% of a moisturizer, 1.6 wt% to 1.7 wt% of an emulsifier, 10 wt% to 11 wt% of a skin moisturizer, 0.15 wt% to 0.25 wt% of a thickener, 0.03 wt% to 0.05 wt% of a chelating agent, and 0.45 wt% to 0.55 wt% of an antioxidant, with the balance being water.

[0019] In an optional embodiment, the humectant includes butylene glycol and 1,2-hexanediol;

[0020] Emulsifiers include cetearyl oleate, sorbitan oleate, and sodium stearoyl glutamate;

[0021] Emollients include dimethicone, isononyl isononanoate, C15-19 alkyl, C10-18 triglycerides, shea butter esters, and squalane;

[0022] Thickeners include ammonium acryloyldimethyl taurate / VP copolymer;

[0023] Chelating agents include disodium EDTA;

[0024] Antioxidants include p-hydroxyacetophenone.

[0025] In an optional embodiment, the preparation of the cosmetic includes: mixing cetearyl oleate, sorbitan oleate, polydimethylsiloxane, isononyl isononanoate, C15-19 alkyl, C10-18 triglycerides, shea butter ester, and squalane at 80°C to 90°C until all components are completely dissolved to obtain an oil phase;

[0026] Butylene glycol, disodium EDTA, ammonium acryloyl dimethyl taurate / VP copolymer, sodium stearoyl glutamate, hydroxyacetophenone, 1,2-hexanediol and water were mixed at 80°C to 90°C until all components were completely dissolved to obtain an aqueous phase.

[0027] The oil phase is added to the aqueous phase to obtain a mixed liquid;

[0028] Cool the mixture to no more than 40°C and add the soothing and repairing composition.

[0029] The beneficial effects of this invention include:

[0030] The soothing and repairing composition provided by this invention contains water lily polysaccharide, silk protein, and Paris polyphylla extract in a mass ratio of (50~250):(100~500):(10~100). This composition can synergistically inhibit the overexpression of key inflammatory factors (such as NO, IL-6, and TNF-α) through multiple mechanisms, while simultaneously promoting skin barrier repair. Therefore, it achieves excellent soothing and repairing effects with low irritation, making it particularly suitable for sensitive and acne-prone skin. Cosmetics containing this soothing and repairing composition also provide good soothing and repairing effects and are similarly suitable for sensitive and acne-prone skin. Attached Figure Description

[0031] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly described below. It should be understood that the following drawings only show some embodiments of the present invention and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0032] Figure 1 This is a graph showing the results of the cytotoxicity test in Experiment Example 1;

[0033] Figure 2 The graph shows the NO content results in Experiment Example 2;

[0034] Figure 3The graph shows the results of IL-6 content in Experiment Example 2;

[0035] Figure 4 The graph shows the results of TNF-α content in Experiment Example 2;

[0036] Figure 5 The image shows the results of the repair efficacy in Experiment Example 3. Detailed Implementation

[0037] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased commercially.

[0038] The following is a detailed description of the soothing and repairing composition provided by the present invention and its application in cosmetics.

[0039] The present invention provides a soothing and repairing composition containing water lily polysaccharide, silk protein and Paris polyphylla extract.

[0040] Among them, water lily polysaccharides have good antioxidant capacity, effectively scavenging intracellular reactive oxygen species (ROS) and reducing oxidative stress damage to the skin. At the same time, they can inhibit the activity of collagenase and elastase, thereby reducing the degradation of collagen and elastin fibers, playing a key role in skin soothing and maintaining elasticity.

[0041] Silk-core protein exhibits excellent biocompatibility and biodegradability. In this invention, silk-core protein exerts its core function through multiple mechanisms: firstly, it can directly eliminate inflammatory mediators; secondly, it can inhibit the excessive secretion of pro-inflammatory factors (such as IL-6 and TNF-α). Furthermore, silk-core protein can stimulate fibroblasts to synthesize collagen and hyaluronic acid, thereby directly promoting the repair and regeneration of the skin barrier while simultaneously combating inflammation.

[0042] Paris polyphylla extract can significantly inhibit the release of inflammatory factors (such as NO, IL-6, and TNF-α) induced by lipopolysaccharide (LPS), thereby effectively alleviating skin inflammation. The Paris polyphylla extract used in this invention contains ≥500 ppm of Paris saponin I for better efficacy.

[0043] In some optional embodiments, the mass ratio of water lily polysaccharide, silk protein and Paris polyphylla extract in the above-mentioned soothing and repairing composition is (50~250):(100~500):(10~100), such as 200:300:80, 50:500:80, 250:100:100, 250:300:10, 200:100:100 or 100:500:50, etc., or other values ​​within the range of (50~250):(100~500):(10~100).

[0044] In some preferred embodiments, the mass ratio of water lily polysaccharide, fibroin and Paris polyphylla extract is (200~250):(100~300):(80~100), such as 200:30:80, 250:100:100, or 200:100:10, etc., or other values ​​within the range of (200~250):(100~300):(80~100).

[0045] In some preferred embodiments, the mass ratio of water lily polysaccharide, silk protein, and Paris polyphylla extract is 200:300:80.

[0046] Continuing on the above, the soothing and repairing composition provided by this invention, by combining water lily polysaccharide, silk protein, and Paris polyphylla extract in the above proportions, can achieve a synergistic effect in soothing and repairing. This soothing and repairing composition can reduce the expression of inflammatory factors, inhibit the occurrence of inflammation, promote cell proliferation and repair, and improve skin inflammation problems to a greater extent.

[0047] In addition, the present invention also provides a cosmetic product containing the above-mentioned soothing and repairing composition.

[0048] The cosmetic product may, by way of example, include serums, and may also include creams, masks, sprays, toners, etc.

[0049] In some optional embodiments, the soothing and repairing composition is present in the cosmetic at a concentration of 0.5 wt% to 1.5 wt%, such as 0.5 wt%, 1 wt%, or 1.5 wt%, or other values ​​within the range of 0.5 wt% to 1.5 wt%. In some preferred embodiments, the soothing and repairing composition is present in the cosmetic at a concentration of 1 wt%.

[0050] In some alternative embodiments, cosmetics may also include ingredients such as moisturizers, emulsifiers, skin-nourishing agents, thickeners, chelating agents, and antioxidants.

[0051] In some alternative embodiments, the cosmetic product may contain 4 wt% to 5 wt% of a moisturizer, 1.6 wt% to 1.7 wt% of an emulsifier, 10 wt% to 11 wt% of a skin moisturizer, 0.15 wt% to 0.25 wt% of a thickener, 0.03 wt% to 0.05 wt% of a chelating agent, and 0.45 wt% to 0.55 wt% of an antioxidant, with the balance being water.

[0052] In some preferred embodiments, the cosmetic product may contain 1 wt% of a soothing and repairing composition, 4.5 wt% of a moisturizer, 1.65 wt% of an emulsifier, 10.5 wt% of a skin moisturizer, 0.2 wt% of a thickener, 0.04 wt% of a chelating agent, and 0.5 wt% of an antioxidant, with the balance being water.

[0053] The humectants may include, for example, butylene glycol and 1,2-hexanediol.

[0054] Emulsifiers may include, for example, cetearyl oleate, sorbitan oleate and sodium stearoyl glutamate.

[0055] Emollients may include, for example, dimethicone, isononyl isononanoate, C15-19 alkyl, C10-18 triglycerides, shea butter esters, and squalane.

[0056] Thickeners may include, for example, ammonium acryloyldimethyl taurate / VP copolymers.

[0057] Chelating agents may include, for example, disodium EDTA.

[0058] Antioxidants may include, for example, p-hydroxyacetophenone.

[0059] In some typical embodiments, the cosmetic may contain 1 wt% of a soothing and repairing composition, 4 wt% of butylene glycol, 0.5 wt% of 1,2-hexanediol, 0.15 wt% of sodium stearoyl glutamate, 0.9 wt% of cetearyl oleate, 0.6 wt% of sorbitan oleate, 2 wt% of polydimethylsiloxane, 2 wt% of isononyl isononanoate, 2 wt% of C15-19 alkyl, 2 wt% of C10-18 triglycerides, 0.5 wt% of shea butter, 2 wt% of squalane, 0.2 wt% of ammonium acryloyl dimethyl taurate / VP copolymer, 0.04 wt% of disodium EDTA, 0.5 wt% of p-hydroxyacetophenone, and the balance being water.

[0060] In some alternative embodiments, the cosmetic can be prepared in the following manner:

[0061] Step 1: Cetearyl oleate, sorbitan oleate, polydimethylsiloxane, isononyl isononanoate, C15-19 alkyl, C10-18 triglycerides, shea butter ester, and squalane are mixed at 80℃~90℃ (e.g., 85℃) until all components are completely dissolved to obtain the oil phase;

[0062] Step 2: Mix butanediol, disodium EDTA, ammonium acryloyldimethyl taurate / VP copolymer, sodium stearoyl glutamate, hydroxyacetophenone, 1,2-hexanediol and water at 80℃~90℃ (e.g., 85℃) until all components are completely dissolved to obtain an aqueous phase;

[0063] Step 3: Add the oil phase to the aqueous phase and homogenize it at a speed of 7800 rpm to 8200 rpm to obtain a mixed liquid.

[0064] Step 4: Cool the mixture to no more than 40°C (e.g., cool to 40°C), add the soothing and repairing composition, stir well, and obtain the cosmetic.

[0065] The features and performance of the present invention will be further described in detail below with reference to embodiments.

[0066] The water lily polysaccharide in the following examples and comparative examples was obtained from the South China Botanical Garden, Chinese Academy of Sciences; the silk heart protein was obtained from SNATE (Suzhou) Biotechnology Co., Ltd.; and the Paris polyphylla extract was obtained from Kunming Kangersun Bioengineering Co., Ltd.

[0067] Example 1 (denoted as S1)

[0068] This embodiment provides a soothing and repairing composition, which, by weight, consists of 200 parts of water lily polysaccharide, 300 parts of silk protein, and 80 parts of Paris polyphylla extract.

[0069] Examples 2-6

[0070] Examples 2-6 (denoted as S2-S6)

[0071] The difference from Example 1 is that the content of water lily polysaccharide, silk protein and Paris polyphylla extract in the soothing and repairing composition is different, as shown in Table 1.

[0072] Comparative Examples 1-5 (denoted as D1-D5)

[0073] The difference between Comparative Examples 1-5 and Example 1 is that the contents of water lily polysaccharide, silk protein and Paris polyphylla extract in the soothing and repairing composition are different, as shown in Table 1.

[0074] Comparative Example 6 (denoted as D6)

[0075] The difference between this comparative example and Example 1 is that the water lily polysaccharide was replaced with an equal amount of aloe polysaccharide.

[0076] Comparative Example 7 (denoted as D7)

[0077] The difference between this comparative example and Example 1 is that the fibronectin was replaced with an equal amount of fibronectin extract.

[0078] Comparative Example 8 (denoted as D8)

[0079] The difference between this comparative example and Example 1 is that the Paris polyphylla extract was replaced with an equal amount of Centella asiatica extract.

[0080] Table 1 Composition

[0081]

[0082] Application examples

[0083] This application example provides a soothing and repairing essence lotion containing 1 wt% of the soothing and repairing composition provided in any one of Examples 1 to 6, 4 wt% of butylene glycol, 0.5 wt% of 1,2-hexanediol, 0.15 wt% of sodium stearoyl glutamate, 0.9 wt% of cetearyl oleate, 0.6 wt% of sorbitan oleate, 2 wt% of polydimethylsiloxane, 2 wt% of isononyl nonanoate, 2 wt% of C15-19 alkyl, 2 wt% of C10-18 triglycerides, 0.5 wt% of shea butter, 2 wt% of squalane, 0.2 wt% of ammonium acryloyl dimethyl taurate / VP copolymer, 0.04 wt% of disodium EDTA, 0.5 wt% of p-hydroxyacetophenone, and the balance being water;

[0084] The preparation of the above-mentioned soothing and repairing essence includes:

[0085] Step 1: Add cetearyl oleate, sorbitan oleate, polydimethylsiloxane, isononyl isononanoate, C15-19 alkyl, C10-18 triglycerides, shea butter ester, and squalane to the oil phase pot according to the formula, heat to 85°C, and stir until all components are completely dissolved to obtain the oil phase;

[0086] Step 2: Add butanediol, disodium EDTA, ammonium acryloyl dimethyl taurate / VP copolymer, sodium stearoyl glutamate, hydroxyacetophenone, 1,2-hexanediol and water to the aqueous phase pot according to the formula, heat to 85°C, and stir until all components are completely dissolved to obtain the aqueous phase;

[0087] Step 3: Add the oil phase to the aqueous phase and homogenize at 8000 rpm to obtain a mixed liquid.

[0088] Step 4: Cool the mixture to 40°C, add the soothing and repairing composition, stir well, and obtain the soothing and repairing essence.

[0089] Experimental Example 1

[0090] The cytotoxicity tests of water lily polysaccharide, filocule protein, and Paris polyphylla extract were performed using the following methods:

[0091] (1) Take the incubated cells, digest the cells with 0.25% trypsin, add serum-containing culture medium to stop digestion after the cells are fully digested, add culture medium and PBS and mix well;

[0092] (2) Centrifuge the cells from step (1) at 1000 r / min for about 5 min, remove the cell supernatant, add fresh culture medium and mix well to make the RAW264.7 cell density 5 × 10⁻⁶. 4 The cells were seeded at 100 μL of cell suspension per well in a 96-well plate and incubated at 37°C in a 5% CO2 incubator.

[0093] (3) Remove the culture medium, wash the cells twice with PBS solution, and add culture medium with sample concentrations of 1000 μg / mL, 500 μg / mL, 250 μg / mL, 100 μg / mL and 10 μg / mL respectively as sample groups. At the same time, control group and blank group are set up. The control group is the group with cells inoculated and full culture medium, and the blank group is the group with no cells inoculated and only full culture medium added. Place the culture plate in a 37℃, 5% CO2 incubator for 24h.

[0094] (4) Remove the cell supernatant, wash the cells three times with PBS solution, add 100 μL of culture medium containing 5 mg / mL MTT to each well, and continue culturing at 37ºC for 4 days. Add 150 μL of DMSO solution and shake for 10 min. Calculate the cell viability (%) using the following formula: (OD value of sample group - OD value of blank group) / (OD value of control group - OD value of blank group) × 100%.

[0095] Cell viability results of water lily polysaccharide, filocule protein and Paris polyphylla extract are as follows: Figure 1 As shown, the cell viability of water lily polysaccharide is >90% in the concentration range of 50 μg / mL to 250 μg / mL; the cell viability of filocule protein is >90% in the concentration range of 100 μg / mL to 500 μg / mL; and the cell viability of Paris polyphylla extract is >90% in the concentration range of 10 μg / mL to 100 μg / mL. Therefore, the compositions of the present invention only need to meet the above safety ranges when used.

[0096] Experimental Example 2

[0097] The samples from each embodiment and comparative example were subjected to experiments to inhibit cellular inflammatory factors.

[0098] (1) NO test: Macrophages were collected, and the density of the cell suspension was approximately 5 × 10⁻⁶. 4 Cells / mL. Add 2 mL of cell suspension to each well of a 6-well plate, resulting in a cell density of 10-1. 5 Cells / well. Set up normal control, model group, and sample group. The normal control group was inoculated with cells in complete culture medium; the model group was inoculated with cells in complete culture medium and subsequently treated with LPS; the sample group was inoculated with cells in culture medium containing different concentrations of the sample and subsequently treated with LPS. Aspirate the culture medium from each well of the 6-well plate. Add 2 mL of culture medium to the normal control and model groups, and 2 mL of sample culture medium to the sample group. Place the 6-well plate in an incubator and continue culturing for 24 hours. After approximately 24 hours of cell culture, aspirate the culture medium from each well of the 6-well plate. Add complete culture medium to the normal control group, and 2 mL of 5 μg / mL LPS culture medium to the model and sample groups. Place the 6-well plate in an incubator and continue culturing for 24 hours. Aspirate 50 μL of supernatant into a 96-cell plate, add Gliese reagent to detect NO content, and measure the absorbance (OD) at 540 nm using a microplate reader. Calculate the OD values ​​of cells after treatment with different concentrations of the test sample, construct a standard curve, and calculate the NO content of the treatment groups.

[0099] The formula for calculating the NO inhibition rate is as follows:

[0100] ;

[0101] In the formula, E represents the NO expression level.

[0102] The effects of different groups on the NO expression levels and inhibition rates in macrophages are shown in Table 2. Figure 2 As shown.

[0103] Table 2 NO content and inhibition rate

[0104]

[0105] From Table 2 and Figure 2 It can be seen that, compared with the blank group, the NO content secreted by cells in the model group was significantly increased. Compared with the model group, the samples in both the comparative and example samples significantly inhibited NO expression and had anti-inflammatory effects.

[0106] (2) IL-6 and TNF-α test: RAW264.7 cells were collected, centrifuged and counted, and 2×10⁻⁶ cells were prepared. 4100 μL of cell suspension was seeded into each well of a 96-well plate; cells were cultured until adherence. Normal control, model, and sample groups were set up. The normal control group consisted of cells seeded with complete culture medium; the model group consisted of cells seeded with complete culture medium and subsequently treated with LPS; the sample groups consisted of cells seeded with culture medium containing different concentrations of samples and subsequently treated with LPS. The culture medium was aspirated from each well of the 96-well plate. 2 mL of culture medium was added to the normal control and model groups, and 2 mL of complete culture medium containing 10% FBS was added to the sample groups. The plates were placed in an incubator and cultured for 24 hours. After approximately 24 hours of cell culture, the culture medium was aspirated from each well of the 96-well plate. Complete culture medium containing 10% FBS was added to the normal control group, and 2 mL of 1 μg / mL LPS culture medium was added to the model and sample groups. The plates were placed in an incubator and cultured for another 24 hours. 50 μL of the supernatant was centrifuged at 1000g for 20 minutes, and the supernatant was used for assay according to the ELISA kit instructions.

[0107] The formulas for calculating the inhibition rates of IL-6 and TNF-α are as follows:

[0108] ;

[0109] In the formula, E represents the expression level of IL-6 or TNF-α.

[0110] The effects of different groups on the expression levels and inhibition rates of IL-6 in macrophages are shown in Table 3. Figure 3 As shown in Table 4, the effects of different groups on the expression level and inhibition rate of TNF-α in macrophages are as follows. Figure 4 As shown.

[0111] Table 3. IL-6 content results

[0112]

[0113] From Table 3 and Figure 3 The results showed that, compared with the control group, the level of IL-6 secreted by cells in the model group was significantly increased. Compared with the model group, both the comparative and experimental groups significantly inhibited IL-6 expression, demonstrating anti-inflammatory effects.

[0114] Table 4. Results of TNF-α content

[0115]

[0116] From Table 4 and Figure 4 The results showed that, compared with the control group, the level of TNF-α secreted by cells in the model group was significantly increased. Compared with the model group, both the comparative and experimental groups significantly inhibited the expression of TNF-α, demonstrating anti-inflammatory effects.

[0117] (3) Calculation of synergy coefficient: Calculate the rate of change of inflammatory factor expression levels in different groups relative to the model group, and calculate the synergy coefficient according to the King's method. The formula is as follows:

[0118] ;

[0119] Among them, E AB E represents the inhibition rate of inflammatory factors by the combination of the two drugs (Examples S1 and S2 of this application). A E represents the inhibition rate of inflammatory factors by a single dose of A (comparative examples D1 and D2 of this application). B The inflammatory factor inhibition rate of single-dose B (comparative examples D4 and D5 of this application);

[0120] If Q < 0.85, it means E A Ingredients and E B The components have antagonistic effects; if Q > 1.15, it indicates that E A Ingredients and E B The components have a synergistic effect; if 0.85 ≤ Q ≤ 1.15, it means E A Ingredients and E B The components have a normal additive effect.

[0121] Based on the data in Tables 2 to 4, and combined with the above formula for calculating the synergy coefficient, the results of the synergy coefficient for the inhibition of inflammatory factors are shown in Table 5.

[0122] Table 5. Coefficients of Inhibition of Inflammatory Factors

[0123]

[0124] As shown in Table 5, the synergistic coefficients of S1 and S2, which consist of water lily polysaccharide, filoin, and Paris polyphylla extract, are all greater than 1.15, proving that under the ratio of water lily polysaccharide, filoin, and Paris polyphylla extract provided by S1 and S2, water lily polysaccharide, filoin, and Paris polyphylla extract can play a synergistic role.

[0125] Referring to Tables 1 to 4, in all embodiments, S1 showed the best effect in inhibiting the expression of NO, IL-6, and TNF-α, while S2 showed the worst effect. Regarding the inhibition of NO expression, there were no significant differences between S1, S3, and S5, and between S2, S4, and S6; that is, S1, S3, and S5 had essentially the same effect on inhibiting NO expression, and S2, S4, and S6 had essentially the same effect on inhibiting NO expression. Similarly, regarding the inhibition of IL-6 expression, there were no significant differences between S1 and S3, and between S2, S4, S5, and S6; that is, S1 and S3 had essentially the same effect on inhibiting IL-6 expression, and S2, S4, S5, and S6 had essentially the same effect on inhibiting IL-6 expression. Likewise, regarding the inhibition of TNF-α expression, there were no significant differences between S2 and S5; that is, S2 and S5 had essentially the same effect on inhibiting TNF-α expression.

[0126] Therefore, within the dosage range of water lily polysaccharide, silk protein, and Paris polyphylla extract provided by this invention, water lily polysaccharide, silk protein, and Paris polyphylla extract can all play a synergistic effect.

[0127] Experimental Example 3

[0128] According to the published methods in the "Test Methods for Seven Efficacy Items of Cosmetics: Anti-wrinkle, Firming, Moisturizing, Oil Control, Repairing, Nourishing, and Soothing", the samples were evaluated for their repairing effects by measuring the transepidermal water loss rate of the skin; and for their soothing effects by conducting a lactic acid stinging test.

[0129] (1) Transepidermal water loss (TEWL) value test

[0130] Number of participants evaluated: 20 in total, 5 males and 15 females, meeting the criteria for voluntary inclusion of participants;

[0131] Assessment areas: left and right cheeks;

[0132] Assessment instrument: Tewameter skin moisture loss test probe ® TM 300;

[0133] Evaluation time points: before using the sample, 15 minutes after using the sample, and 2 hours after using the sample;

[0134] Evaluation sample: an essence lotion containing 1 wt% of the S1 composition, the formulation of which is shown in Table 6.

[0135] Table 6 Composition

[0136]

[0137] The evaluation results are shown in Table 7 and Figure 5 As shown.

[0138] Table 7. Transepidermal water loss (TEWL) values ​​of skin (g / h / m²) 2 Evaluation results

[0139]

[0140] (2) Lactic acid stinging test

[0141] Number of participants evaluated: 20 in total, 5 males and 15 females, meeting the criteria for voluntary inclusion of participants;

[0142] Assessment method: At room temperature, 50 μL of 10% lactic acid solution was dropped onto the nasolabial fold on either side. Subjects were asked about their subjective symptoms at 2.5 min and 5 min, respectively, and scored on a scale of 0 to 3. The scores at the two questioning points were then added together to obtain the result of the lactic acid stinging test.

[0143] Assessment criteria: 0 points for no stinging sensation, 1 point for mild stinging sensation, 2 points for moderate stinging sensation, and 3 points for severe stinging sensation.

[0144] Evaluation time points: before using the sample, and 2 hours after using the sample;

[0145] Evaluation sample: serum containing 1 wt% of the S1 composition (same as above);

[0146] The evaluation results are shown in Table 8.

[0147] Table 8. Evaluation Results of Lactic Acid Sting Test

[0148]

[0149] As can be seen from Tables 7 and 8, the serum containing 1 wt% of the S1 composition has a good repairing and soothing effect.

[0150] In summary, the soothing and repairing composition provided by this invention can synergistically inhibit the overexpression of key inflammatory factors (such as NO, IL-6, and TNF-α) through multiple mechanisms, while promoting the repair of the skin barrier. Thus, it achieves excellent soothing and repairing effects with low irritation, making it particularly suitable for sensitive and acne-prone skin. Cosmetics containing the above-mentioned soothing and repairing composition can also achieve good soothing and repairing effects and are similarly suitable for sensitive and acne-prone skin.

[0151] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A soothing repair composition characterized in that, The soothing and repairing composition consists of water lily polysaccharide, silk protein, and Paris polyphylla extract; The mass ratio of the water lily polysaccharide, the silk heart protein, and the Paris polyphylla extract is (50~250):(100~500):(10~100); The content of Paris saponin I in the Paris extract is ≥500ppm.

2. The soothing repair composition of claim 1, wherein The mass ratio of the water lily polysaccharide, the silk protein, and the Paris polyphylla extract is (200~250):(100~300):(80~100).

3. The soothing repair composition of claim 1, wherein The mass ratio of the water lily polysaccharide, the silk protein, and the Paris polyphylla extract is 200:300:

80.

4. A cosmetic product, characterized by, The cosmetic contains the soothing and repairing composition according to any one of claims 1 to 3.

5. The cosmetic product according to claim 4, characterized in that, The soothing and repairing composition is present in the cosmetic at a concentration of 0.5 wt% to 1.5 wt%.

6. The cosmetic product according to claim 4 or 5, characterized in that, The cosmetics also include moisturizers, emulsifiers, skin-nourishing agents, thickeners, chelating agents, and antioxidants.

7. The cosmetic product according to claim 6, characterized in that, The cosmetic contains 4 wt% to 5 wt% of the moisturizer, 1.6 wt% to 1.7 wt% of the emulsifier, 10 wt% to 11 wt% of the skin moisturizer, 0.15 wt% to 0.25 wt% of the thickener, 0.03 wt% to 0.05 wt% of the chelating agent, and 0.45 wt% to 0.55 wt% of the antioxidant, with the balance being water.

8. The cosmetic product according to claim 7, characterized in that, The humectant includes butylene glycol and 1,2-hexanediol; The emulsifiers include cetearyl oleate, sorbitan oleate and sodium stearoyl glutamate; The emollients include polydimethylsiloxane, isononyl isononanoate, C15-19 alkyl, C10-18 triglycerides, shea butter esters, and squalane. The thickener includes ammonium acryloyldimethyl taurate / VP copolymer; The chelating agent includes disodium EDTA; The antioxidants include p-hydroxyacetophenone.

9. The cosmetic product according to claim 8, characterized in that, The preparation of the cosmetic comprises: mixing the cetearyl oleate, the sorbitan oleate, the polydimethylsiloxane, the isononyl isononanoate, the C15-19 alkyl, the C10-18 triglyceride, the shea butter ester, and the squalane at 80°C to 90°C until all components are completely dissolved to obtain an oil phase; The butanediol, disodium EDTA, ammonium acryloyldimethyl taurate / VP copolymer, sodium stearoyl glutamate, hydroxyacetophenone, 1,2-hexanediol, and water are mixed at 80°C to 90°C until all components are completely dissolved to obtain an aqueous phase. The oil phase is added to the aqueous phase to obtain a mixed liquid; Cool the mixture to no more than 40°C and add the soothing and repairing composition.

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