Lipase catalyzed grease flavor enhancing device

Abstract

The invention relates to the technical field of grease processing, in particular to a lipase-catalyzed grease flavor enhancing device which comprises an enzyme catalysis tank, a continuous enzymolysis structure and a drainage mixing mechanism which are used for achieving a hydrolysis-esterification cascade reaction on grease are arranged in the enzyme catalysis tank, and a tank body supporting mechanism used for circularly receiving materials is arranged outside the enzyme catalysis tank. The drainage mixing mechanism can adjust the flowing speed of the grease emulsion in the enzyme catalysis tank, and the controllable feeding mechanism can adjust the enzymolysis speed of the continuous enzymolysis structure and the drainage mixing mechanism by monitoring the concentration of flavor substances. The continuous enzymolysis structure is arranged, efficient directional enhancement of the flavor of the grease is achieved through the synergistic effect of the multi-stage enzymolysis structure (non-specific hydrolysis-> 1, 3-site specific hydrolysis-> esterification) and the dynamic magnetic field control technology, the magnetic field adjustable design can significantly reduce the pressure drop, and can effectively improve the mass transfer efficiency, so that the flavor of the grease is improved. The contradiction between mass transfer limitation and high energy consumption of a traditional reactor is solved.

Description

Technical Field

[0001] This invention relates to the field of oil processing technology, and more particularly to an oil flavor enhancement device catalyzed by lipase. Background Technology

[0002] With the continuous development of the food industry, consumers have increasingly higher requirements for the flavor and quality of edible oils and oily foods. Although traditional oil processing (such as refining and deodorizing processes) can extend the shelf life, it will lead to the loss of natural flavor substances and problems such as monotonous flavor and bland taste. Therefore, in the current oil processing field, improving the flavor characteristics of oils through lipase catalytic reaction is a common and important technical means.

[0003] However, enhancing the flavor of oils and fats using lipase-catalyzed reactions requires completing three key steps in sequence: triglyceride hydrolysis, monoglyceride formation, and flavor ester synthesis. Most existing flavor enhancement devices use a scheme of multiple single-function reactors in series, which results in insufficient synergy of multi-stage reactions. Moreover, the enzyme carrier is in a static filling state, and high-viscosity oils are prone to forming a boundary layer effect when flowing through the carrier layer, which leads to a significant reduction in the contact efficiency between the substrate and the enzyme active site, resulting in obvious mass transfer limitations. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a lipase-catalyzed oil flavor enhancement device, which solves the technical problems of insufficient multi-stage synergy and significant mass transfer limitations in existing flavor enhancement devices. It has the advantages of effectively improving multi-stage synergy and increasing mass transfer efficiency.

[0005] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a lipase-catalyzed oil flavor enhancement device, comprising an enzyme catalytic tank, wherein the interior of the enzyme catalytic tank is provided with a continuous enzymatic hydrolysis structure for realizing a hydrolysis-esterification cascade reaction of oil and a flow guiding and mixing mechanism, both ends of the enzyme catalytic tank are provided with controllable feeding mechanisms, and the exterior of the enzyme catalytic tank is provided with a tank support mechanism for circulating material reception. The flow guiding and mixing mechanism can adjust the flow rate of the oil emulsion within the enzyme catalytic tank, and the controllable feeding mechanism can adjust the enzymatic hydrolysis rate of the continuous enzymatic hydrolysis structure and the flow guiding and mixing mechanism by monitoring the concentration of flavor compounds. After the emulsion enters the enzyme catalytic vessel, it undergoes hydrolysis and esterification reactions sequentially under the action of a continuous enzymatic hydrolysis structure and a flow-guiding mixing mechanism, thereby simultaneously enhancing the umami and fruity flavors of the oil. The continuous enzymatic hydrolysis structure includes a primary, secondary, and tertiary enzymatic hydrolysis structure, which are arranged vertically from top to bottom inside the enzyme catalytic vessel. The primary enzymatic hydrolysis structure includes a detachable catalyst bed assembly installed inside the enzyme catalytic vessel, with a carrier structure inside the catalyst bed assembly for enzymatically hydrolyzing the oil emulsion. After entering the enzyme catalytic vessel, the oil emulsion flows through the inside of the catalyst bed assembly, where the enzyme comes into contact with the oil emulsion.

[0006] Preferably, the carrier structure consists of a magnetic core, a core-shell, and an enzyme immobilization layer. The magnetic core is made of iron oxide to provide superparamagnetism, and the core-shell is made of silicon dioxide to protect the magnetic core from corrosion by the reaction solution and to provide -OH groups for enzyme immobilization. The enzyme immobilization layer is formed by immobilizing lipase using glutaraldehyde crosslinking.

[0007] Preferably, the catalyst bed assembly is provided with an electromagnetic component for providing a magnetic field. The electromagnetic component is provided with an adjustment knob for adjusting the magnetic field strength. The electromagnetic component consists of an electromagnetic coil and a PLC controller. The electromagnetic coil is wrapped around the outside of the catalyst bed assembly. The PLC controller can control the current intensity and direction on the electromagnetic coil.

[0008] Preferably, the flow mixing mechanism includes a flow-guiding ring shroud fixedly installed on the inner wall of the enzyme catalytic vessel, an electrically telescopic rod fixedly installed on the catalytic bed assembly, and a horn-shaped flow-guiding platform provided on the electrically telescopic rod, the horn-shaped flow-guiding platform being coaxially arranged with the flow-guiding ring shroud. The cross-sectional diameter of the flow-guiding ring shroud increases sequentially from bottom to top, and the cross-sectional diameter of the horn-shaped flow-guiding platform increases sequentially from top to bottom.

[0009] Preferably, the enzyme catalytic vessel is equipped with a detachable liquid distribution platform inside. The liquid distribution platform has rectangular channels at equal intervals, and the middle of the liquid distribution platform is recessed downward and has a drainage channel. After the oil emulsion falls from the funnel-shaped drainage platform, it will flow downward along the liquid distribution platform.

[0010] Preferably, the controllable feeding mechanism includes an upper closed end cap and a lower closed end cap symmetrically arranged at both ends of the enzyme catalytic tank. A feeding pipe is fixedly installed on the upper closed end cap, and a discharge valve assembly is fixedly installed on the lower closed end cap. The feeding pipe extends into the interior of the upper closed end cap and is fixedly installed with a dispersing nozzle. An aeration ring pipe is fixedly installed on the inner side of the upper closed end cap, and an air inlet pipe for supplying nitrogen to the aeration ring pipe is fixedly installed on the outer side of the upper closed end cap. When the oil emulsion enters the enzyme catalytic tank through the feeding pipe, it will fall evenly onto the primary enzymatic hydrolysis structure under the action of the dispersing nozzle.

[0011] Preferably, the discharge valve assembly is equipped with a GC sensor for detecting the concentration of flavor compounds. The GC sensor can provide feedback to adjust the aeration rate of the aeration ring pipe, thereby providing feedback to adjust the flow rate of the oil emulsion and ensuring the effect of flavor enhancement.

[0012] Preferably, the secondary and tertiary enzymatic hydrolysis structures are identical in structure to the primary enzymatic hydrolysis structure.

[0013] Preferably, the tank support mechanism includes a support frame for fixing the enzyme catalytic tank, a rotating bracket is movably mounted on the support frame, and a receiving round tank is fixedly mounted on the rotating bracket. After the flavor-enhanced oil flows out from the lower end of the enzyme catalytic tank, it will enter the interior of the receiving round tank. After the receiving round tank is full, the rotating bracket will automatically move the receiving round tank out.

[0014] Preferably, a drive motor for driving the rotating bracket is fixedly installed at the lower end of the support frame, and several receiving cylinders are provided to realize cyclic material receiving, which can effectively improve the material receiving efficiency.

[0015] By employing the above technical solution, the present invention provides a lipase-catalyzed oil flavor enhancement device, which has at least the following beneficial effects: 1. By setting up a continuous enzymatic hydrolysis structure, the present invention achieves efficient and targeted enhancement of oil flavor through the synergistic effect of multi-stage enzymatic hydrolysis structure (non-specific hydrolysis → 1, 3-position specific hydrolysis → esterification) and dynamic magnetic field control technology. Furthermore, the adjustable magnetic field design can significantly reduce pressure drop and effectively improve mass transfer efficiency, thus solving the contradiction between limited mass transfer and high energy consumption in traditional reactors.

[0016] 2. By setting up a continuous enzymatic hydrolysis structure and using a combination of modular, detachable catalyst bed components and magnetically immobilized enzyme carriers, this invention can not only effectively shorten the carrier replacement time, but also ensure that the enzyme retains more than 90% of its activity after being reused ten times. In addition, the through-hole anti-clogging design can be adapted to customized flavor enhancement of oils with different viscosities, and has the advantages of high-efficiency production and low-cost maintenance.

[0017] 3. By setting up a flow-guiding and mixing mechanism, this invention utilizes the synergistic effect between the flow-guiding ring and the trumpet-shaped flow-guiding platform to achieve bidirectional flow mixing of the oil emulsion in the enzyme catalytic tank (alternating radial inward and outward flow). This allows the enzymatically hydrolyzed products to fully contact and react. Moreover, as the oil emulsion falls through the separation platform, it is forcibly dispersed into multiple fine streams, ensuring its uniform distribution to the next enzymatic hydrolysis structure. This avoids the problem of uneven local concentrations common in traditional devices, thereby increasing the efficiency of the secondary and tertiary enzymatic hydrolysis reactions by more than 30%.

[0018] 4. By setting up a flow-guiding and mixing mechanism, and utilizing the cooperation between the horn-shaped flow-guiding platform and the electric telescopic rod, the present invention can flexibly adjust the mixing and distribution parameters according to the different viscosities of greases, and can also change the falling trajectory of the grease emulsion by adjusting the stroke of the electric telescopic rod, so that the device can adapt to the processing conditions of various greases.

[0019] 5. This invention, by setting up a controllable feeding mechanism, forms a microbubble flow through the uniform liquid distribution of the dispersing nozzle and the nitrogen injection of the aeration ring pipe. This enables the construction of a highly efficient gas-liquid-solid three-phase reaction system in the enzyme catalytic tank. This design not only effectively prevents scaling on the enzyme carrier surface, but also increases the mass transfer rate of hydrophobic substrates by 2.5 times due to the turbulence effect of the microbubbles. It is especially suitable for the deep enzymatic hydrolysis of high-viscosity oils, and the reaction efficiency can be increased by more than 40% compared with the traditional liquid phase system.

[0020] 6. By setting up a controllable feeding mechanism and utilizing the cooperation between the GC sensor and the aeration ring, when the concentration of key flavor compounds (such as hexanal) is detected to be below the threshold, the system will automatically reduce the aeration rate to prolong the substrate residence time, which can largely ensure the stability of the flavor enhancement effect (product flavor concentration fluctuation ≤5%).

[0021] 7. By setting up a tank support mechanism and through the coordinated design of a rotating bracket and multiple receiving round tanks, this invention achieves continuous collection and seamless production of flavor-enhancing oils. When a single receiving round tank is full, the rotating bracket will automatically move it out and switch to an empty tank to continue receiving. The entire process requires no manual intervention, and the receiving efficiency is increased by more than 60%. It avoids the production interruption problem caused by traditional single-tank collection and is particularly suitable for the needs of large-scale continuous industrial production. Attached Figure Description

[0022] The accompanying drawings, which are included to provide a further understanding of this application and form part of this application, illustrate exemplary embodiments of this application and are used to explain this application, but do not constitute an undue limitation of this application. In the drawings: Figure 1 This is a perspective view of the overall structure of the present invention; Figure 2 This is a schematic diagram of the enzyme catalytic vessel in this invention; Figure 3 This is a schematic diagram of the continuous enzymatic hydrolysis structure in this invention; Figure 4 This is a schematic diagram of the primary enzymatic hydrolysis structure in this invention; Figure 5 This is a schematic diagram of the catalytic bed assembly in this invention; Figure 6 This is a schematic diagram of the carrier structure in this invention; Figure 7 This is a schematic diagram of the flow mixing mechanism in this invention; Figure 8 This is a schematic diagram of the horn-shaped drainage platform in this invention; Figure 9 This is a schematic diagram of the liquid separation platform in this invention; Figure 10 This is a schematic diagram of the controllable feeding mechanism in this invention; Figure 11 This is a schematic diagram of the tank support mechanism in this invention.

[0023] In the diagram: 1. Enzyme catalytic vessel; 2. Continuous enzymatic hydrolysis structure; 201. Primary enzymatic hydrolysis structure; 2011. Catalytic bed assembly; 2012. Electromagnetic assembly; 2013. Adjustment knob; 2014. Carrier structure; A. Magnetic core; B. Core-shell; C. Enzyme immobilization layer; 202. Secondary enzymatic hydrolysis structure; 203. Tertiary enzymatic hydrolysis structure; 3. Inlet mixing mechanism; 301. Inlet ring hood; 302. Horn-shaped inlet stage; 303. Separation platform; 304, electric telescopic rod; 305, rectangular through-channel; 4, controllable feeding mechanism; 401, upper closed end cap; 402, feeding pipe; 403, dispersion nozzle; 404, aeration ring pipe; 405, air inlet pipe; 406, discharge valve assembly; 407, GC sensor; 408, lower closed end cap; 5, tank support mechanism; 501, support frame; 502, rotating bracket; 503, receiving round tank. Detailed Implementation

[0024] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0025] Example 1

[0026] Most existing flavor enhancement devices employ a series of multiple single-function reactors, resulting in insufficient synergy in multi-stage reactions. Furthermore, the enzyme carrier is in a statically packed state, and high-viscosity oils easily form a boundary layer effect as they flow through the carrier layer, significantly reducing the contact efficiency between the substrate and the enzyme's active site, leading to obvious mass transfer limitations. To address this technical deficiency in existing technologies, such as... Figures 1-6 As shown in the figure, this embodiment proposes a lipase-catalyzed oil flavor enhancement device. Through the synergistic effect of a multi-stage enzymatic hydrolysis structure (non-specific hydrolysis → 1, 3-position specific hydrolysis → esterification) and dynamic magnetic field control technology, efficient and directional enhancement of oil flavor is achieved. The device has an enzyme catalysis tank 1 with a continuous enzymatic hydrolysis structure 2 and a flow mixing mechanism 3 for realizing the hydrolysis-esterification cascade reaction of oil. The enzyme catalysis tank 1 has controllable feeding mechanisms 4 at both ends. The enzyme catalysis tank 1 has a tank support mechanism 5 for circulating material reception on the outside. The flow mixing mechanism 3 can adjust the flow rate of the oil emulsion in the enzyme catalysis tank 1. The controllable feeding mechanism 4 can adjust the enzymatic hydrolysis rate of the continuous enzymatic hydrolysis structure 2 and the flow mixing mechanism 3 by monitoring the concentration of flavor compounds. After the oil emulsion enters the interior of the enzyme catalysis tank 1, it will undergo hydrolysis and esterification reactions in sequence under the action of the continuous enzymatic hydrolysis structure 2 and the flow mixing mechanism 3, thereby simultaneously enhancing the umami and fruity flavor of the oil.

[0027] Specifically, the continuous enzymatic hydrolysis structure 2 includes a primary enzymatic hydrolysis structure 201, a secondary enzymatic hydrolysis structure 202, and a tertiary enzymatic hydrolysis structure 203. The secondary and tertiary enzymatic hydrolysis structures 202 and 203 have the same structure as the primary enzymatic hydrolysis structure 201. The primary, secondary, and tertiary enzymatic hydrolysis structures 201, 202, and 203 are arranged vertically from top to bottom inside the enzyme catalytic vessel 1. The primary enzymatic hydrolysis structure 201 includes a catalytic bed assembly 2011 that is detachably installed inside the enzyme catalytic vessel 1. The catalytic bed assembly 2011 is equipped with an electromagnetic component 2012 for providing a magnetic field. The electromagnetic component 2012 is equipped with an adjustment knob 2013 for adjusting the magnetic field strength. The electromagnetic component 2012 consists of an electromagnetic coil and a PLC controller. An electromagnetic coil is wound around the outside of the catalytic bed assembly 2011. The PLC controller can control the current intensity and direction on the electromagnetic coil. The interior of the catalytic bed assembly 2011 is equipped with a carrier structure 2014 for enzymatic hydrolysis of the oil emulsion. The carrier structure 2014 consists of a magnetic core A, a core-shell B, and an enzyme immobilization layer C. The magnetic core A is made of iron oxide to provide superparamagnetism. The core-shell B is made of silicon dioxide to protect the magnetic core A from corrosion by the reaction solution and to provide -OH groups for enzyme immobilization. The enzyme immobilization layer C is formed by immobilizing lipase through glutaraldehyde crosslinking. After the oil emulsion enters the interior of the enzyme catalytic tank 1, it flows through the interior of the catalytic bed assembly 2011. During the flow through the catalytic bed assembly 2011, the enzyme comes into contact with the oil emulsion.

[0028] As can be seen from the above, after the oil emulsion enters the interior of the enzyme catalytic tank 1, it will pass through the interior of the primary enzymatic hydrolysis structure 201, the secondary enzymatic hydrolysis structure 202, and the tertiary enzymatic hydrolysis structure 203 in sequence.

[0029] like Figure 4 , Figure 5 As shown, the catalytic bed assembly 2011 is hollow inside and contains several carrier structures 2014. Multiple circular through holes are opened on both the upper and lower sides. Therefore, the oil emulsion will enter the interior of the catalytic bed assembly 2011 through the circular through holes. Then, the oil emulsion will rapidly hydrolyze triglycerides under the action of highly active non-specific lipases (such as Lipase AY).

[0030] Subsequently, the oil emulsion flowing out from the primary enzymatic hydrolysis structure 201 enters the interior of the secondary enzymatic hydrolysis structure 202. At this time, the 1,3-position specific lipase on the enzyme immobilization layer C inside the secondary enzymatic hydrolysis structure 202 reacts with the oil emulsion again, thereby directionally generating monoglycerides (MAG).

[0031] Next, the oil emulsion flowing out from the secondary enzymatic hydrolysis structure 202 will enter the interior of the tertiary enzymatic hydrolysis structure 203. At this time, the esterification enzyme on the enzyme immobilization layer C inside the secondary enzymatic hydrolysis structure 202 will catalyze the reaction between FFA and alcohol to generate flavor esters, thereby realizing the hydrolysis-esterification cascade reaction, which can simultaneously enhance the umami and fruity flavor of the oil.

[0032] Moreover, during the feeding stage, the electromagnetic component 2012 applies a weak magnetic field to the outer periphery of the catalytic bed component 2011. At this time, the carrier structure 2014 is magnetically adsorbed and fixed, forming a quasi-fixed bed with a relatively low pressure drop. During the enzymatic reaction stage, the electromagnetic component 2012 intermittently applies strong magnetic field pulses under the action of the PLC controller. At this time, the carrier structure 2014 will briefly detach from the adsorption site and roll in the fluid, so that the oil and water droplets can fully contact the enzyme.

[0033] This embodiment achieves highly efficient and targeted enhancement of oil flavor through the synergistic effect of a continuous enzymatic hydrolysis structure 2 and a multi-stage enzymatic hydrolysis structure (non-specific hydrolysis → 1, 3-position specific hydrolysis → esterification) and dynamic magnetic field control technology. Furthermore, the adjustable magnetic field design significantly reduces pressure drop and effectively improves mass transfer efficiency, resolving the contradiction between limited mass transfer and high energy consumption in traditional reactors. In addition, by setting up the continuous enzymatic hydrolysis structure 2 and using a combination of modular and detachable catalyst bed assembly 2011 and magnetically immobilized enzyme carrier, this embodiment not only effectively shortens the carrier replacement time but also ensures that the enzyme retains more than 90% of its activity after ten reuses. Combined with the through-hole anti-clogging design, it can be adapted to customized flavor enhancement of oils with different viscosities, offering the advantages of high-efficiency production and low-cost maintenance.

[0034] Example 2

[0035] To enable bidirectional flow mixing (alternating radial inward and outward flow) of the oil emulsion within enzyme catalytic tank 1, and to ensure sufficient contact and reaction of the enzymatically hydrolyzed products, based on Example 1, as follows... Figure 3 as well as Figures 7-9 As shown, this embodiment includes a flow-guiding and mixing mechanism 3. Specifically, the flow-guiding and mixing mechanism 3 includes a flow-guiding ring cover 301 fixedly installed on the inner wall of the enzyme catalytic tank 1. An electric telescopic rod 304 is fixedly installed on the catalytic bed assembly 2011. A trumpet-shaped flow-guiding platform 302 is provided on the electric telescopic rod 304. The trumpet-shaped flow-guiding platform 302 is coaxially arranged with the flow-guiding ring cover 301. The cross-sectional diameter of the flow-guiding ring cover 301 increases from bottom to top, and the cross-sectional diameter of the trumpet-shaped flow-guiding platform 302 increases from top to bottom. A liquid-dispensing platform 303 is detachably installed inside the enzyme catalytic tank 1. Rectangular through grooves 305 are evenly spaced on the liquid-dispensing platform 303. The middle of the liquid-dispensing platform 303 is recessed downward and has a drainage channel. After the oil emulsion falls from the trumpet-shaped flow-guiding platform 302, it will flow downward along the liquid-dispensing platform 303.

[0036] As can be seen from the above, after the oil emulsion flows downward from the catalyst bed assembly 2011, the oil emulsion far from the axis of the enzyme catalytic tank 1 will flow inward under the guidance of the guide ring 301 and fall onto the funnel-shaped guide platform 302, while the oil emulsion close to the axis of the enzyme catalytic tank 1 will gradually flow outward under the action of the funnel-shaped guide platform 302, so that the enzymatically hydrolyzed oil emulsion is fully mixed between the guide ring 301 and the funnel-shaped guide platform 302.

[0037] Next, the mixed oil emulsion will drip down along the lower edge of the funnel-shaped drainage platform 302, as... Figure 9 As shown, after the oil emulsion falls, it flows from the periphery of the separation platform 303 towards the center. During the flow, part of the oil emulsion falls downward through the rectangular channel 305, and another part of the oil emulsion falls downward through the drainage channel, so that the oil emulsion can fall more dispersedly into the interior of the secondary enzymatic hydrolysis structure 202 or the tertiary enzymatic hydrolysis structure 203.

[0038] Moreover, the height of the horn-shaped drainage platform 302 can be freely adjusted by the electric telescopic rod 304. When encountering grease with high viscosity, the staff will control the electric telescopic rod 304 to extend downward, thereby increasing the space between the horn-shaped drainage platform 302 and the drainage ring cover 301 to avoid blockage.

[0039] This embodiment, by setting up a flow-guiding and mixing mechanism 3, utilizes the synergistic effect between the flow-guiding ring 301 and the trumpet-shaped flow-guiding platform 302 to achieve bidirectional flow mixing of the oil emulsion within the enzyme catalytic tank 1 (alternating radial inward and outward flow). This ensures that the enzymatically hydrolyzed products can fully contact and react. Furthermore, as the oil emulsion falls through the separation platform 303, it is forcibly dispersed into multiple fine streams, ensuring its uniform distribution to the next enzymatic hydrolysis structure. This avoids the problem of uneven local concentrations common in traditional devices, improving the efficiency of the secondary and tertiary enzymatic hydrolysis reactions by more than 30%. In addition, by setting up the flow-guiding and mixing mechanism 3, this embodiment utilizes the cooperation between the trumpet-shaped flow-guiding platform 302 and the electric telescopic rod 304. This allows for flexible adjustment of mixing and distribution parameters according to oils of different viscosities, and also allows for changing the falling trajectory of the oil emulsion by adjusting the stroke of the electric telescopic rod 304, enabling the device to adapt to various oil processing conditions.

[0040] Example 3

[0041] To enable the device to be applied to the deep enzymatic hydrolysis of high-viscosity oils and to broaden its applicability, based on the above embodiments, as follows: Figure 1 , Figure 2 , Figure 3 , Figure 7 as well as Figure 10 As shown, this embodiment proposes a controllable feeding mechanism 4. Specifically, the controllable feeding mechanism 4 includes an upper closed end cap 401 and a lower closed end cap 408 symmetrically arranged at both ends of the enzyme catalytic tank 1. A feed pipe 402 is fixedly installed on the upper closed end cap 401, and a discharge valve assembly 406 is fixedly installed on the lower closed end cap 408. The feed pipe 402 extends into the interior of the upper closed end cap 401 and a dispersion nozzle 403 is fixedly installed thereon. An aeration ring pipe 404 is fixedly installed on the inner side of the upper closed end cap 401. An air inlet pipe 405 is fixedly installed on the outside of the cover 401 to supply nitrogen into the aeration ring pipe 404. When the oil emulsion enters the enzyme catalysis tank 1 through the feed pipe 402, it will fall evenly onto the primary enzymatic hydrolysis structure 201 under the action of the dispersion nozzle 403. The discharge valve assembly 406 is equipped with a GC sensor 407 for detecting the concentration of flavor compounds. The GC sensor 407 can provide feedback to adjust the aeration rate of the aeration ring pipe 404, thereby providing feedback to adjust the flow rate of the oil emulsion and ensuring the effect of flavor enhancement.

[0042] As can be seen from the above, during the feeding process, the oil emulsion will fall evenly onto the primary enzymatic hydrolysis structure 201 through the feed pipe 402 and the dispersion nozzle 403. At the same time, the nitrogen gas inside the air inlet pipe 405 will be sprayed downward through the aeration ring pipe 404, thereby forming a gas-liquid-solid three-phase flow inside the enzyme catalytic tank 1, which can prevent scaling on the surface of the enzyme carrier and promote the transfer of hydrophobic substrates.

[0043] Furthermore, during the discharge process, the GC sensor 407 can detect the concentration of key flavor compounds (hexanal / ethyl acetate) in the product. When the concentration is low, the aeration rate of the aeration ring 404 will be reduced under the action of the PLC controller, thereby reducing the flow rate of the oil emulsion and increasing the contact reaction time between the oil emulsion and the enzyme.

[0044] This embodiment, by setting up a controllable feeding mechanism 4, forms a microbubble flow through the uniform liquid distribution of the dispersing nozzle 403 and the nitrogen injection of the aeration ring pipe 404. This enables the construction of a highly efficient gas-liquid-solid three-phase reaction system within the enzyme catalytic tank 1. This design not only effectively prevents scaling on the enzyme carrier surface, but also increases the mass transfer rate of hydrophobic substrates by 2.5 times due to the turbulence effect of the microbubbles. It is particularly suitable for the deep enzymatic hydrolysis of high-viscosity oils, and the reaction efficiency can be improved by more than 40% compared to traditional liquid-phase systems. In addition, by setting up a controllable feeding mechanism 4, this embodiment utilizes the interaction between the GC sensor 407 and the aeration ring pipe 404. When the concentration of key flavor compounds (such as hexanal) is detected to be below the threshold, the system will automatically reduce the aeration rate to prolong the substrate residence time, which can largely ensure the stability of the flavor enhancement effect (product flavor concentration fluctuation ≤5%).

[0045] Example 4

[0046] To improve the material receiving efficiency of the device and achieve continuous production, based on the above embodiments, such as... Figure 1 and Figure 11 As shown, this embodiment includes a tank support mechanism 5. Specifically, the tank support mechanism 5 includes a support frame 501 for fixing the enzyme catalytic tank 1. A rotating bracket 502 is movably mounted on the support frame 501, and a receiving round tank 503 is fixedly mounted on the rotating bracket 502. After the flavor-enhanced oil flows out from the lower end of the enzyme catalytic tank 1, it enters the interior of the receiving round tank 503. When the receiving round tank 503 is full, the rotating bracket 502 will automatically move the receiving round tank 503 out. A drive motor for driving the rotating bracket 502 is fixedly mounted at the lower end of the support frame 501. Several receiving round tanks 503 are provided to realize cyclic receiving, which can effectively improve the receiving efficiency.

[0047] This embodiment achieves continuous collection and seamless production of flavor-enhancing oils by setting up a tank support mechanism 5 and through the coordinated design of a rotating bracket 502 and multiple receiving round tanks 503. When a single receiving round tank 503 is full, the rotating bracket 502 will automatically move it out and switch to an empty tank to continue receiving. The whole process does not require manual intervention, and the receiving efficiency is improved by more than 60%. It avoids the production interruption problem caused by traditional single-tank collection and is particularly suitable for the needs of large-scale continuous industrial production.

[0048] The control method of this invention is automatic control through a controller. The control circuit of the controller can be implemented by simple programming by those skilled in the art. The power supply is also common knowledge in the art. Furthermore, since this invention is mainly used to protect mechanical devices, the control method and circuit connection will not be explained in detail here.

[0049] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0050] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A lipase-catalyzed oil flavor enhancement device, comprising an enzyme catalytic tank (1), wherein the enzyme catalytic tank (1) is externally provided with a tank support mechanism (5) for circulating material reception, characterized in that: The enzyme catalytic tank (1) is equipped with a continuous enzymatic hydrolysis structure (2) and a flow mixing mechanism (3) for realizing the hydrolysis-esterification cascade reaction of oils and fats. The flow mixing mechanism (3) can adjust the flow rate of the oil emulsion in the enzyme catalytic tank (1). The enzyme catalytic tank (1) is equipped with a controllable feeding mechanism (4) at both ends. The controllable feeding mechanism (4) can adjust the enzymatic hydrolysis rate of the continuous enzymatic hydrolysis structure (2) and the flow mixing mechanism (3) by monitoring the concentration of flavor compounds. The continuous enzymatic hydrolysis structure (2) includes a primary enzymatic hydrolysis structure (201), a secondary enzymatic hydrolysis structure (202), and a tertiary enzymatic hydrolysis structure (203). The primary enzymatic hydrolysis structure (201), the secondary enzymatic hydrolysis structure (202), and the tertiary enzymatic hydrolysis structure (203) are arranged vertically from top to bottom inside the enzyme catalytic tank (1). The primary enzymatic hydrolysis structure (201) includes a catalytic bed assembly (2011) that can be detachably installed inside the enzyme catalytic tank (1). The catalytic bed assembly (2011) has a carrier structure (2014) for enzymatic hydrolysis of oil emulsions inside.

2. The lipase-catalyzed oil flavor enhancement device according to claim 1, characterized in that: The carrier structure (2014) consists of a magnetic core A, a nucleoshell B, and an enzyme immobilization layer C.

3. The lipase-catalyzed oil flavor enhancement device according to claim 1, characterized in that: The catalyst bed assembly (2011) is provided with an electromagnetic component (2012) for providing a magnetic field, and the electromagnetic component (2012) is provided with an adjustment knob (2013) for adjusting the magnetic field strength.

4. The lipase-catalyzed oil flavor enhancement device according to claim 1, characterized in that: The flow mixing mechanism (3) includes a flow ring cover (301) fixedly installed on the inner wall of the enzyme catalytic tank (1), an electric telescopic rod (304) fixedly installed on the catalytic bed assembly (2011), and a horn-shaped flow platform (302) provided on the electric telescopic rod (304). The horn-shaped flow platform (302) is coaxially arranged with the flow ring cover (301).

5. The lipase-catalyzed oil flavor enhancement device according to claim 4, characterized in that: The enzyme catalytic vessel (1) is equipped with a detachable liquid separation platform (303), and rectangular through slots (305) are provided at equal intervals on the liquid separation platform (303).

6. The lipase-catalyzed oil flavor enhancement device according to claim 1, characterized in that: The controllable feeding mechanism (4) includes an upper closed end cap (401) and a lower closed end cap (408) symmetrically arranged at both ends of the enzyme catalytic tank (1). A feed pipe (402) is fixedly installed on the upper closed end cap (401), and a discharge valve assembly (406) is fixedly installed on the lower closed end cap (408). The feed pipe (402) extends into the interior of the upper closed end cap (401) and a dispersion nozzle (403) is fixedly installed thereon. An aeration ring pipe (404) is fixedly installed on the inner side of the upper closed end cap (401), and an air inlet pipe (405) for supplying nitrogen to the aeration ring pipe (404) is fixedly installed on the outer side of the upper closed end cap (401).

7. The lipase-catalyzed oil flavor enhancement device according to claim 6, characterized in that: The discharge valve assembly (406) is equipped with a GC sensor (407) for detecting the concentration of flavorings. The GC sensor (407) can provide feedback to adjust the aeration rate of the aeration ring pipe (404).

8. The lipase-catalyzed oil flavor enhancement device according to claim 1, characterized in that: The secondary enzymatic hydrolysis structure (202), the tertiary enzymatic hydrolysis structure (203) have the same structure as the primary enzymatic hydrolysis structure (201).

9. The lipase-catalyzed oil flavor enhancement device according to claim 1, characterized in that: The tank support mechanism (5) includes a support frame (501) for fixing the enzyme catalytic tank (1), a rotating bracket (502) is movably mounted on the support frame (501), and a receiving round tank (503) is fixedly mounted on the rotating bracket (502).

10. The lipase-catalyzed oil flavor enhancement device according to claim 9, characterized in that: The lower end of the support frame (501) is fixedly equipped with a drive motor for driving the rotating bracket (502), and several receiving round tanks (503) are provided.

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