Method and device for determining reference value of dissolved oxygen in water environment

Abstract

The invention provides a method and a device for determining a reference value of dissolved oxygen in a water environment. The method comprises the following steps: determining a species average acute value and a species average chronic value corresponding to each tested species, and a genus average acute value and a genus average chronic value of the genus of the tested species; the genus average acute values and the genus average chronic values are ranked according to the toxicity sequence from high to low, and ranks are distributed to the genus corresponding to the ranked genus average acute values and the genus corresponding to the ranked genus average chronic values; determining final acute values and final chronic values of all tested species; and respectively determining an acute dissolved oxygen reference concentration, an acute dissolved oxygen reference saturation, a chronic dissolved oxygen reference concentration and a chronic dissolved oxygen reference saturation based on the obtained final acute value and the final chronic value. The method can provide reference for scientific evaluation of water quality and aquatic organism protection.

Description

Technical Field

[0001] This invention belongs to the field of water quality protection technology, and in particular relates to a method and apparatus for determining the dissolved oxygen benchmark value in aquatic environments. Background Technology

[0002] Dissolved oxygen (DO) is molecular oxygen dissolved in water, and it plays a vital role in improving water quality, maintaining the health of aquatic ecosystems, and protecting biodiversity. When the DO concentration in water bodies falls below 2 mg / L, the survival of most organisms is threatened, leading to reduced biodiversity and blackened, odorous water bodies. With the continuous upgrading of water pollution control technologies, the quality of surface water in my country has improved significantly in recent years. However, due to differences in climate, geographical conditions, and pollutant sources in different regions / basins, DO concentrations vary considerably; furthermore, different aquatic organisms have different DO requirements.

[0003] Currently, my country lacks published DO benchmark values ​​(i.e., the minimum concentration or level of DO in freshwater environments that does not have harmful effects on organisms and their ecological functions) that conform to the characteristics of regional water environments and biota. The existing "Surface Water Environmental Quality Standard" (GB 3838—2002) uses foreign benchmark values ​​for DO and adopts a unified value nationwide, but it lacks relevant scientific basis for research on my country's aquatic biota, water environment characteristics and natural background, as well as water ecological effects.

[0004] Researching methods for determining the baseline value of dissolved oxygen in the aquatic environment will help guide the protection of aquatic life and coordinate the integrated management of regions / watersheds. Summary of the Invention

[0005] This invention provides a method and apparatus for determining the baseline value of dissolved oxygen in aquatic environments, in order to solve the above-mentioned technical problems.

[0006] According to a first aspect of the present invention, a method for determining a dissolved oxygen baseline value in an aquatic environment is provided, the method comprising the following steps: Step S1: Identify the test species in the aquatic environment. The test species reflect the characteristics of the aquatic flora and represent different ecological trophic levels. Obtain comprehensive environmental-aquatic organism toxicity data, which includes toxicity data and environmental data. Determine the species and genus corresponding to the test species. Step S2: Determine the species mean acute value and species mean chronic value for each tested species, and the genus mean acute value and genus mean chronic value for the genus to which each tested species belongs. Step S3: Sort the average acute value and average chronic value of the genera in descending order of toxicity, and assign ranks to the genera corresponding to the average acute value and the genera corresponding to the average chronic value of the genera. When ranking the genus-average acute values, the final acute value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average acute value; when ranking the genus-average chronic values, the final chronic value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average chronic value. Step S4: Determine the acute dissolved oxygen baseline concentration and the chronic dissolved oxygen baseline concentration based on the obtained final acute value and final chronic value, respectively; determine the acute dissolved oxygen baseline saturation based on the acute dissolved oxygen baseline concentration and the saturated dissolved oxygen concentration, and determine the chronic dissolved oxygen baseline saturation based on the chronic dissolved oxygen baseline concentration and the saturated dissolved oxygen concentration.

[0007] Preferably, in step S1, the test species include at least 8 species and cover the following biological groups: bony fish of the Cyprinidae family, bony fish of non-cyprinidae family, and benthic or planktonic crustaceans.

[0008] Preferably, step S2: determine the species-average acute value and species-average chronic value for each tested species, and the genus-average acute value and genus-average chronic value for the genus to which each tested species belongs, wherein: The formula for calculating the species mean acute value of the tested species is:

[0009] in, Let be the species-average acute toxicity value corresponding to the i-th tested species, k represent the acute toxicity effect category (dimensionless), and m represent the number of acute toxicity values ​​for species i. This represents the p-th acute toxicity value of species i, expressed in mg / L. ; The formula for calculating the genus-average acute value of the tested species is:

[0010] Where j is the genus number of the tested species. Let be the genus average acute value for the j-th genus, and 'a' be the number of species included in the j-th genus. Let be the species-average acute value corresponding to the q-th species of the j-th genus. ; The formula for calculating the chronic toxicity value of the test species is as follows:

[0011] in, denoted as the chronic toxicity value for the i-th tested species, and z represents the category of chronic toxicity effect, which is dimensionless. The highest observed concentration of dissolved oxygen for the i-th tested species is given in mg / L. This represents the lowest concentration of dissolved oxygen for the i-th test species with no observable effect, expressed in mg / L. The formula for calculating the species mean chronicity value of the tested species is:

[0012] in, is the species-average chronicity value corresponding to the i-th tested species; n represents the number of chronicity toxicity values ​​for species i. This represents the p1th chronic toxicity value of species i, expressed in mg / L. ; The formula for calculating the genus-average chronicity value of the tested species is as follows:

[0013] in, Let be the genus average chronicity value for the j-th genus. Let be the species-average chronicity value corresponding to the q-th species of the j-th genus. .

[0014] Preferably, in step S3, when ranking the genus-average acute values, the final acute value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average acute value; when ranking the genus-average chronic values, the final chronic value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average chronic value, including: Step S31: When ranking the genus-average acute values, calculate the cumulative acute frequency of each tested species within its genus:

[0015] in, R represents the acute cumulative frequency of the genus to which the tested species belongs, R represents the rank of the genus to which the tested species belongs, and N represents the total number of all genera. The genera containing the four tested species with the smallest distance at the 95% acute cumulative frequency were selected as the first candidate genera, and the acute cumulative frequency of the first candidate genera and the genus average acute value of the genera containing the first candidate genera were retained. Step S32: Calculate the final acute value based on the first candidate genus. :

[0016]

[0017]

[0018] and These are all intermediate quantities obtained from calculations. This is the average acute value; Step S33: When ranking the genus-average chronicity values, calculate the cumulative frequency of chronicity for each tested species within its genus:

[0019] in, The chronic cumulative frequency of the genus to which the tested species belong; The genera containing the four tested species with the smallest distance at the 95% chronic accumulation frequency were selected as second candidate genera. The chronic accumulation frequency of the second candidate genera and the genus average chronic value of the genera containing the second candidate genera were retained. Step S34: Calculate the final chronic value based on the second candidate genus. :

[0020]

[0021]

[0022] and Both 2 are intermediate quantities obtained from calculation. This is the average chronic value.

[0023] Preferably, step S4: determining the acute dissolved oxygen baseline concentration and the chronic dissolved oxygen baseline concentration based on the obtained final acute value and final chronic value, respectively; determining the acute dissolved oxygen baseline saturation based on the acute dissolved oxygen baseline concentration and the saturated dissolved oxygen concentration, and determining the chronic dissolved oxygen baseline saturation based on the chronic dissolved oxygen baseline concentration and the saturated dissolved oxygen concentration, includes: For the final acute value, Multiply by a correction factor to obtain the acute dissolved oxygen baseline concentration; where the correction factor is the LC5 / LC5 ratio of all tested species. 50 Mean; LC5 is the 5% lethal concentration, LC 50 This is the median lethal concentration; For the final chronic value, As a baseline concentration for chronic dissolved oxygen; The acute dissolved oxygen baseline saturation is determined based on the acute dissolved oxygen baseline concentration and the saturated dissolved oxygen concentration, and the chronic dissolved oxygen baseline saturation is determined based on the chronic dissolved oxygen baseline concentration and the saturated dissolved oxygen concentration.

[0024] According to a second aspect of the present invention, an apparatus for determining a dissolved oxygen baseline value in an aquatic environment is provided, the apparatus comprising: Data acquisition module: Configured to identify test species in the aquatic environment, which reflect the characteristics of aquatic flora and represent different ecological trophic levels; acquire integrated environmental-aquatic organism toxicity data, which includes toxicity data and environmental data; and determine the species and genus corresponding to the test species. Acute and chronic value calculation module: configured to determine the species average acute value, species average chronic value, genus average acute value, and genus average chronic value of each tested species. Sorting module: Configured to sort the average acute value and average chronic value of genera in descending order of toxicity, and assign ranks to the genera corresponding to the average acute value and the genera corresponding to the average chronic value of genera after sorting. When ranking the genus-average acute values, the final acute value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average acute value; when ranking the genus-average chronic values, the final chronic value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average chronic value. Dissolved oxygen baseline concentration determination module: configured to determine acute dissolved oxygen baseline concentration and chronic dissolved oxygen baseline concentration based on the obtained final acute value and final chronic value respectively; determine acute dissolved oxygen baseline saturation based on acute dissolved oxygen baseline concentration and saturated dissolved oxygen concentration; and determine chronic dissolved oxygen baseline saturation based on chronic dissolved oxygen baseline concentration and saturated dissolved oxygen concentration.

[0025] Preferably, the data acquisition module includes at least eight species and covers the following biological groups: cyprinid fish, non-cyprinid fish, and benthic or planktonic crustaceans.

[0026] Preferably, the determination of the species-average acute value, species-average chronic value, genus-average acute value, and genus-average chronic value of each tested species, wherein: The formula for calculating the species mean acute value of the tested species is:

[0027] in, Let be the species-average acute toxicity value corresponding to the i-th tested species, k represent the acute toxicity effect category (dimensionless), and m represent the number of acute toxicity values ​​for species i. This represents the p-th acute toxicity value of species i, expressed in mg / L. ; The formula for calculating the genus-average acute value of the tested species is:

[0028] Where j is the genus number of the tested species. Let be the genus average acute value for the j-th genus, and 'a' be the number of species included in the j-th genus. Let be the species-average acute value corresponding to the q-th species of the j-th genus. ; The formula for calculating the chronic toxicity value of the test species is as follows:

[0029] in, denoted as the chronic toxicity value for the i-th tested species, and z represents the category of chronic toxicity effect, which is dimensionless. The highest observed concentration of dissolved oxygen for the i-th tested species is given in mg / L. This represents the lowest concentration of dissolved oxygen for the i-th test species with no observable effect, expressed in mg / L. The formula for calculating the species mean chronicity value of the tested species is:

[0030] in, is the species-average chronicity value corresponding to the i-th tested species; n represents the number of chronicity toxicity values ​​for species i. This represents the p1th chronic toxicity value of species i, expressed in mg / L. ; The formula for calculating the genus-average chronicity value of the tested species is as follows:

[0031] in, Let be the genus average chronicity value for the j-th genus. Let be the species-average chronicity value corresponding to the q-th species of the j-th genus. .

[0032] Preferably, when ranking the genus-average acute values, the final acute value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average acute value; when ranking the genus-average chronic values, the final chronic value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average chronic value, including: When ranking the genus-average acute values, the cumulative frequency of acute values ​​for each tested species within its genus is calculated:

[0033] in, R represents the acute cumulative frequency of the genus to which the tested species belongs, R represents the rank of the genus to which the tested species belongs, and N represents the total number of all genera. The genera containing the four tested species with the smallest distance at the 95% acute cumulative frequency were selected as the first candidate genera, and the acute cumulative frequency of the first candidate genera and the genus average acute value of the genera containing the first candidate genera were retained. The final acute value was calculated based on the first candidate genus. :

[0034]

[0035]

[0036] and These are all intermediate quantities obtained from calculations. This is the average acute value; When ranking the genus-average chronicity values, the cumulative frequency of chronicity for each tested species within its genus is calculated:

[0037] in, The chronic cumulative frequency of the genus to which the tested species belong; The genera containing the four tested species with the smallest distance at the 95% chronic accumulation frequency were selected as second candidate genera. The chronic accumulation frequency of the second candidate genera and the genus average chronic value of the genera containing the second candidate genera were retained. The final chronicity value was calculated based on the second candidate genus. :

[0038]

[0039]

[0040] and Both 2 are intermediate quantities obtained from calculation. This is the average chronic value.

[0041] Preferably, the determination of acute dissolved oxygen baseline concentration and chronic dissolved oxygen baseline concentration based on the obtained final acute value and final chronic value, respectively; the determination of acute dissolved oxygen baseline saturation based on acute dissolved oxygen baseline concentration and saturated dissolved oxygen concentration, and the determination of chronic dissolved oxygen baseline saturation based on chronic dissolved oxygen baseline concentration and saturated dissolved oxygen concentration, include: For the final acute value, Multiply by a correction factor to obtain the acute dissolved oxygen baseline concentration; where the correction factor is the LC5 / LC5 ratio of all tested species. 50 Mean; LC5 is the 5% lethal concentration, LC 50 This is the median lethal concentration; For the final chronic value, As a baseline concentration for chronic dissolved oxygen; Acute dissolved oxygen baseline saturation is determined based on acute dissolved oxygen baseline concentration and saturated dissolved oxygen concentration; chronic dissolved oxygen baseline saturation is determined based on chronic dissolved oxygen baseline concentration and saturated dissolved oxygen concentration. According to a third aspect of the present invention, an electronic device is provided, comprising: A processor is used to execute multiple instructions; Memory, used to store multiple instructions; The plurality of instructions are to be stored in the memory and loaded and executed by the processor as described above.

[0042] According to a fourth aspect of the present invention, a computer-readable storage medium is provided, wherein a plurality of instructions are stored therein; the plurality of instructions are configured to be loaded by a processor and executed as described above.

[0043] This invention employs a toxicity percentage ranking method. By ranking the toxicity data of different species within their genera, the percentile of each toxicity value in the overall distribution is calculated. Data from the most sensitive genera is then selected to derive acute and chronic dissolved oxygen concentrations and saturation levels that meet the growth and metabolic needs of most (e.g., 95%) aquatic organisms as benchmark values.

[0044] The present invention has the following beneficial technical effects: Compared to existing standards (HJ 831—2022, HJ 1260—2022, etc.) and related technical documents, which either only address pollutants or are only applicable to local aquatic organisms for determining aquatic environmental benchmarks, this invention proposes a screening method for native test species sensitive to dissolved oxygen, and HOEC. DO NOEC DO The concept of effective concentration applicable to dissolved oxygen is used, and a more scientifically grounded method for deriving dissolved oxygen benchmarks in aquatic environments is proposed using the toxicity percentage ranking method. This method can provide guidance for the derivation of dissolved oxygen benchmarks in aquatic environments and provide a reference for the scientific evaluation of water quality and the protection of aquatic life.

[0045] The above description is merely an overview of the technical solution of the present invention. In order to better understand the technical means of the present invention and to implement it in accordance with the contents of the specification, the preferred embodiments of the present invention are described in detail below with reference to the accompanying drawings. Attached Figure Description

[0046] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention, and the invention is illustrated by the following drawings. In the drawings: Figure 1 This is a schematic flowchart illustrating a method for determining the dissolved oxygen baseline value in aquatic environments according to one embodiment of the present invention.

[0047] Figure 2This is a schematic diagram of the acute toxicity-cumulative frequency distribution of aquatic organisms according to the present invention.

[0048] Figure 3 This is a schematic diagram of the cumulative frequency distribution of chronic toxicity to aquatic organisms according to the present invention. Detailed Implementation

[0049] First, combine Figure 1 This invention describes a method for determining the dissolved oxygen baseline value in aquatic environments according to one embodiment of the present invention. For example... Figure 1 As shown, the method includes the following steps: Step S1: Identify the test species in the aquatic environment. The test species reflect the characteristics of the aquatic flora and represent different ecological trophic levels. Obtain comprehensive environmental-aquatic organism toxicity data, which includes toxicity data and environmental data. Determine the species and genus corresponding to the test species. Step S2: Determine the species mean acute value and species mean chronic value for each tested species, and the genus mean acute value and genus mean chronic value for the genus to which each tested species belongs. Step S3: Sort the average acute value and average chronic value of the genera in descending order of toxicity, and assign ranks to the genera corresponding to the average acute value and the genera corresponding to the average chronic value of the genera. When ranking the genus-average acute values, the final acute value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average acute value; when ranking the genus-average chronic values, the final chronic value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average chronic value. Step S4: Determine the acute dissolved oxygen baseline concentration and the chronic dissolved oxygen baseline concentration based on the obtained final acute value and final chronic value, respectively; determine the acute dissolved oxygen baseline saturation based on the acute dissolved oxygen baseline concentration and the saturated dissolved oxygen concentration, and determine the chronic dissolved oxygen baseline saturation based on the chronic dissolved oxygen baseline concentration and the saturated dissolved oxygen concentration.

[0050] In this invention, environmental data includes altitude data, water temperature data, and salinity data.

[0051] Furthermore, in step S1, the test species include at least 8 species and cover the following biological groups: bony fish of the Cyprinidae family, bony fish of non-cyprinidae families, and benthic or planktonic crustaceans.

[0052] Aquatic environmental data refers to aquatic environmental data that meets the requirements of data quality specifications. These specifications are pre-defined and include experimental requirements for acquiring the aquatic environmental data, conditions for using test organisms, exposure conditions for test organisms, constraints on toxicity data, and priorities for toxicity data. The exposure conditions are as follows: for acute toxicity data, the exposure time for test organisms is 24–96 hours; for chronic toxicity data, the exposure time for test organisms is 14–21 days or covers a sensitive life stage (embryo, larva, etc.).

[0053] In this invention, step S1 includes: The selection of test species shall be carried out. Test species shall meet the following conditions: (1) They shall reflect the composition characteristics of the aquatic flora of the study area / basin, represent different ecological trophic levels, and include at least 8 species covering the following 3 biological groups: 1 species of cyprinid fish, 1 species of non-cyprinid cyprinid fish, and 1 species of benthic or planktonic crustacean; (2) They shall be relatively sensitive to dissolved oxygen and have standard or mature testing methods; (3) For rare and endangered species or endemic species in my country, they shall be selected selectively in accordance with the relevant laws and regulations on wildlife protection. Clearly define the data sources. Data sources mainly include: (1) toxicity data from toxicity databases or peer-reviewed publicly published literature or reports; (2) toxicity data obtained through supplementary testing using standard testing methods during the derivation of baseline values. Test methods refer to standard documents such as GB / T 13266—91, GB / T 13267—91, and GB / T 16125—2012. The toxicity data from the sources were screened. The toxicity data included two categories: acute toxicity data and chronic toxicity data. (1) Acute toxicity data were generally survival data, and the effect indicators included the 5% lethal concentration (LC5, the concentration that causes 5% of individuals in a group of test organisms to die) and the median lethal concentration (LC5). 50 (1) The concentration of an indicator that causes half of the individuals in a group of test organisms to die; (2) Chronic toxicity data are generally divided into two categories: growth (weight, body length, growth rate, etc.) and reproduction (hatching rate, hatching time, sex ratio, etc.). The effect indicators include the lowest no-observable concentration of dissolved oxygen (NOEC). DO The lowest dissolved oxygen concentration that did not produce a significant effect on the test organism, such as inhibition of growth rate or hatching rate, compared with the control, and the highest observed effect concentration of dissolved oxygen (HOEC). DO (3) Usually, at least 8 sets (including acute and chronic) toxicity data are required to conduct baseline value derivation in order to identify data distribution and trend of change to a minimum. Clearly define the content of data quality specifications. The following five aspects should be referenced: (1) Experimental design. The experimental design should be based on national or international standard toxicity test methods, or refer to relevant literature from other standard organizations or countries. Otherwise, the experimental design should be explained in detail. A blank control group should be set up in the experiment. A certain number of parallels should be set up for acute and chronic toxicity experiments. The number of parallels should generally be in accordance with the relevant provisions of the standard method for toxicity testing of test organisms (GB / T 13266-91, GB / T 13267-91, etc.). The relevant experimental process and experimental results should be recorded in detail. (2) Test organism, i.e. the conditions of use of test organisms. Information on test organisms should be provided, including Chinese scientific name, common name, Latin name, taxonomic status, living habits, geographical distribution area, habitat characteristics, source (laboratory, breeding base, wild), and life stage. Before the experiment begins, the test organisms should be domesticated under experimental conditions. The mortality rate of standard test organisms during domestication should meet the requirements of the test method. The domestication mortality rate of non-standard test organisms should be ≤10%. (3) Exposure conditions, i.e. the exposure conditions of test organisms. The water quality conditions of the experimental system should be kept stable within a certain range according to the survival requirements of the test organisms; acute toxicity experiments can be conducted using flow-through, semi-static, or static exposure methods, while chronic toxicity experiments generally use flow-through or semi-static exposure methods; different species have different life cycles. Under normal circumstances, for acute toxicity data, the exposure time of the test organisms is 24-96 h; for chronic toxicity data, the exposure time of the test organisms is 14-21 d or covers a certain sensitive life stage (embryo, larva, etc.); (4) Data analysis, i.e., toxicity data constraints. The range of variation of mortality rate, etc. of the control group should comply with the provisions of the standard toxicity test method, and the survival rate of the control group should generally be ≥90%; when the experimental data of the same toxicity endpoint (biological survival, growth, reproduction, etc.) of the same species differ greatly, outliers should be removed based on professional judgment or by using statistical methods; (5) Data priority, i.e., toxicity data priority. In terms of life cycle stage, toxicity data from relatively sensitive life cycle stages > toxicity data from relatively insensitive life cycle stages; in terms of exposure mode, toxicity data from continuous exposure > toxicity data from semi-static exposure > toxicity data from static exposure; generally, the priority of data is determined by comprehensively considering the order of life cycle stage and exposure mode, and the baseline value is derived using the data with higher priority.

[0054] Further, step S2: determining the species-average acute value and species-average chronic value for each tested species, and the genus-average acute value and genus-average chronic value for the genus to which each tested species belongs, wherein: The formula for calculating the species mean acute value of the tested species is:

[0055] in, Let be the species-average acute toxicity value corresponding to the i-th tested species, k represent the acute toxicity effect category (dimensionless), and m represent the number of acute toxicity values ​​for species i. This represents the p-th acute toxicity value of species i, expressed in mg / L. ; The formula for calculating the genus-average acute value of the tested species is:

[0056] Where j is the genus number of the tested species. Let be the genus average acute value for the j-th genus, and 'a' be the number of species included in the j-th genus. Let be the species-average acute value corresponding to the q-th species of the j-th genus. ; The formula for calculating the chronic toxicity value of the test species is as follows:

[0057] in, denoted as the chronic toxicity value for the i-th tested species, and z represents the category of chronic toxicity effect, which is dimensionless. The highest observed concentration of dissolved oxygen for the i-th tested species is given in mg / L. This represents the lowest concentration of dissolved oxygen for the i-th test species with no observable effect, expressed in mg / L. The formula for calculating the species mean chronicity value of the tested species is:

[0058] in, is the species-average chronicity value corresponding to the i-th tested species; n represents the number of chronicity toxicity values ​​for species i. This represents the p1th chronic toxicity value of species i, expressed in mg / L. ; The formula for calculating the genus-average chronicity value of the tested species is as follows:

[0059] in, Let be the genus average chronicity value for the j-th genus. Let be the species-average chronicity value corresponding to the q-th species of the j-th genus. .

[0060] In this invention, the calculation of acute toxicity values ​​distinguishes the effect category of the test species (hatching, growth rate inhibition, movement inhibition, and increased mortality, etc.) and uses LC... 50 As the acute toxicity value (ATV, i.e., the toxicity value of a certain indicator to produce a certain effect on the test organism under short-term exposure conditions).

[0061] Chronic toxicity value calculation. Based on the NOECs for a specific chronic toxicity effect (such as growth or reproductive inhibition) of a species obtained from the same toxicity exposure experiment. DO and HOEC DO The chronic toxicity value (CTV, the toxicity value of a certain indicator to the test organism under long-term exposure conditions) of this effect of the species is calculated.

[0062] Further, in step S3, the average acute value and average chronic value of genera are arranged in descending order of toxicity (i.e., dissolved oxygen concentration from low to high). Ranks are assigned to the genera corresponding to the sorted average acute value and the genera corresponding to the average chronic value. When arranging the average acute value of genera in descending order of toxicity, genera with the same average acute value are arranged in consecutive ranks. When arranging the average chronic value of genera in descending order of toxicity, genera with the same average chronic value are arranged in consecutive ranks. The rank is an integer greater than 0, and the higher the rank, the smaller the rank value.

[0063] Further, in step S3, when ranking the genus-average acute values, the final acute value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average acute value; when ranking the genus-average chronic values, the final chronic value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average chronic value, including: Step S31: When ranking the genus-average acute values, calculate the cumulative acute frequency of each tested species within its genus:

[0064] in, R represents the acute cumulative frequency of the genus to which the tested species belongs, R represents the rank of the genus to which the tested species belongs, and N represents the total number of all genera. The genera containing the four tested species with the smallest distance at the 95% acute cumulative frequency were selected as the first candidate genera, and the acute cumulative frequency of the first candidate genera and the genus average acute value of the genera containing the first candidate genera were retained. Step S32: Calculate the final acute value based on the first candidate genus. :

[0065]

[0066]

[0067] and These are all intermediate quantities obtained from calculations. This is the average acute value; Step S33: When ranking the genus-average chronicity values, calculate the cumulative frequency of chronicity for each tested species within its genus:

[0068] in, The chronic cumulative frequency of the genus to which the tested species belong; The genera containing the four tested species with the smallest distance at the 95% chronic accumulation frequency were selected as second candidate genera. The chronic accumulation frequency of the second candidate genera and the genus average chronic value of the genera containing the second candidate genera were retained. Step S34: Calculate the final chronic value based on the second candidate genus. :

[0069]

[0070]

[0071] and Both 2 are intermediate quantities obtained from calculation. This is the average chronic value.

[0072] In this invention, the first candidate genera and the second candidate genera are the most sensitive genera.

[0073] Further, step S4: determining the acute dissolved oxygen baseline concentration and the chronic dissolved oxygen baseline concentration based on the obtained final acute value and final chronic value, respectively; determining the acute dissolved oxygen baseline saturation based on the acute dissolved oxygen baseline concentration and the saturated dissolved oxygen concentration, and determining the chronic dissolved oxygen baseline saturation based on the chronic dissolved oxygen baseline concentration and the saturated dissolved oxygen concentration, including: For the final acute value, Multiply by a correction factor to obtain the acute dissolved oxygen baseline concentration; where the correction factor is the LC5 / LC5 ratio of all tested species. 50 Mean; LC5 is the 5% lethal concentration, LC 50 This is the median lethal concentration; For the final chronic value, As a baseline concentration for chronic dissolved oxygen; The acute dissolved oxygen baseline saturation is determined based on the acute dissolved oxygen baseline concentration and the saturated dissolved oxygen concentration, and the chronic dissolved oxygen baseline saturation is determined based on the chronic dissolved oxygen baseline concentration and the saturated dissolved oxygen concentration.

[0074] In this invention, This represents the concentration that is harmful to 50% of species, so it needs to be multiplied by the LC5 / LC5 ratio for all species. 50 The mean is adjusted.

[0075] Furthermore, for aquatic environments containing rare and endangered species, if the species' average acute threshold is greater than [a certain value], [further details are needed]. If the species-average chronic value of a rare and endangered species is greater than the final acute value (FCV), then a specific final acute value (FAV) is calculated for that rare and endangered species. 1 And / or the final chronic value FCV1, where:

[0076]

[0077] in, This represents the species-average acute value for this rare and endangered species. This represents the species-average chronicity value for this rare and endangered species. Will Multiply by a correction factor to obtain the specific acute dissolved oxygen baseline concentration for this rare and endangered species, where the correction factor is the LC5 / LC5 ratio of this rare and endangered species. 50 ; Will This serves as a specific chronic dissolved oxygen baseline concentration for this rare and endangered species.

[0078] In this invention, the LC5 / LC5 ratio of this rare and endangered species 50 The data are based on relevant toxicological experimental results from laboratory-bred populations. All experiments must be conducted in accordance with relevant national laws and regulations on wildlife protection and laboratory animal management, and the experimental design and operation must comply with animal ethics requirements.

[0079] In this invention, the baseline saturation is calculated based on the baseline dissolved oxygen concentration and the saturated dissolved oxygen concentration (the dissolved oxygen concentration when oxygen exchange between water and the atmosphere is in equilibrium). For general rivers, lakes, and reservoirs with high salinity, different formulas are used to calculate the saturated dissolved oxygen concentration:

[0080]

[0081] In the formula, DO satSaturated dissolved oxygen concentration (mg / L); h represents altitude (m); T represents multi-year average water temperature (°C); S represents salinity (dimensionless). The formula primarily references the saturated dissolved oxygen concentration calculation formula for rivers and low-salinity lakes and reservoirs in HJ 2.3, with atmospheric pressure corrections based on Henry's Law. Since the source of the formulas is not explicitly stated in either the 1993 or 2018 versions of HJ 2.3, the calculation formulas for saturated dissolved oxygen concentration are verified using data from HJ 506 regarding the functional relationship between oxygen solubility and water temperature / salinity, and the corresponding values ​​of average atmospheric pressure and altitude. The calculated coefficients of determination (R²) between the results of the two formulas and the laboratory measured data are shown. 2 The values ​​all reached 0.999, indicating the reliability of the formula. The percentage ratio of the acute and chronic dissolved oxygen baseline concentrations to the saturated dissolved oxygen concentrations was then calculated to obtain the acute and chronic dissolved oxygen baseline saturation.

[0082] When conducting relevant studies on regions / basins with large spatial spans or significant seasonal differences, it is recommended to use the baseline saturation as the benchmark value to eliminate background concentration differences caused by natural factors such as water temperature, altitude, and salinity.

[0083] By adopting the above technical solution, the main technical advancements of this invention compared to existing technologies are as follows: Compared to existing standards (HJ 831—2022, HJ 1260—2022, etc.) and related technical documents (US EPA's "Guidelines for deriving numerical national water quality criteria for the protection of aquatic organisms and their uses," etc.), which either only target pollutants or are only applicable to the derivation process of water environment benchmarks for local aquatic organisms, this invention proposes a screening method for native test species sensitive to dissolved oxygen, and HOEC... DO NOEC DO The concept of effective concentration applicable to dissolved oxygen is used, and a more scientifically grounded method for deriving dissolved oxygen benchmarks in aquatic environments (including a benchmark concentration derivation method and a benchmark saturation derivation method combining saturated dissolved oxygen concentration) is proposed using the toxicity percentage ranking method. This method can provide guidance for deriving dissolved oxygen benchmarks in aquatic environments and provide a reference for the scientific evaluation of water quality and the protection of aquatic life.

[0084] This invention expands the application of methods such as toxicity percentage ranking in the derivation of dissolved oxygen baseline values ​​(including baseline concentration and saturation), and proposes a method for calculating baseline saturation based on dissolved oxygen baseline concentration. It can provide a solution for the derivation of dissolved oxygen baseline values ​​in aquatic environments and provide a reference for aquatic organism protection, aquatic ecological environment management, and scientific evaluation of water quality.

[0085] In this invention, the method for calculating acute toxicity values ​​should distinguish between effect categories (hatching, growth, kinetic inhibition, and death, etc.) and LC values ​​should be used accordingly. 50 As the survival-based acute toxicity value (ATV, the toxicity value of a certain index that produces a certain effect on a test organism at a certain growth stage under short-term exposure conditions, such as increased mortality), the species-average acute toxicity value (SMAV) for each species and the genus-average acute toxicity value (GMAV) are calculated. The calculation of chronic toxicity values ​​should be based on the NOECs for a specific chronic toxicity effect (growth or reproduction) of a species obtained from the same toxicity exposure experiment. DO and HOEC DO Calculate the chronic toxicity value (CTV) for this effect in the species (CTV is the toxic value of a certain indicator producing a certain effect, such as growth rate inhibition, on a test organism at a certain growth stage under long-term exposure conditions). Differentiate the effect categories, calculate the species-average chronic toxicity value (SMCV) for each species using the CTV calculated from the chronic toxicity data, and then calculate the genus-average chronic toxicity value (GMCV).

[0086] Based on LC 50 The FAV value calculated from the data represents the concentration that is harmful to 50% of species, and needs to be multiplied by the LC5 / LC5 ratio for all species. 50 The mean was corrected to obtain the concentration that protects 95% of species as the acute dissolved oxygen baseline concentration; the calculated FCV does not need to be corrected and is regarded as the chronic dissolved oxygen baseline concentration. For areas / basins with rare and endangered species, if the SMAV (SMCV) of the rare and endangered species is higher than the calculated FAV (FCV), then a specific FAV (FCV) and acute and chronic dissolved oxygen baseline concentrations should be calculated for the rare and endangered species.

[0087] The acute and chronic dissolved oxygen baseline concentrations are combined with the saturated dissolved oxygen concentration, and their ratio percentages are calculated to obtain the acute and chronic dissolved oxygen baseline saturation. The final derived dissolved oxygen baseline value includes the acute baseline value and the chronic baseline value, and the acute and chronic baseline concentrations are generally taken as the baseline values. When conducting relevant studies in areas / basins with large spatial spans or seasonal differences, it is recommended to use the baseline saturation as the baseline value to eliminate background concentration differences caused by natural factors such as water temperature, altitude, and salinity.

[0088] This invention provides an embodiment illustrating a method for determining a dissolved oxygen baseline value in an aquatic environment.

[0089] S1. Determine the test species in the aquatic environment. The test species reflect the characteristics of the aquatic flora and represent different ecological trophic levels. Obtain comprehensive environmental-aquatic organism toxicity data, which includes toxicity data and environmental data. Determine the species and genus corresponding to the test species.

[0090] In this embodiment, according to relevant requirements, nine species (such as Daphnia magna, Amphipoda, and Silver Carp) that can reflect the characteristics of aquatic biota in the Yangtze and Yellow River basins and represent different ecological trophic levels were selected. Based on toxicity data reported in relevant literature and supplemented by testing toxicity data of important native species (while recording or inferring the life stage of the test organisms through weight, body length, etc.), 44 groups of acute (1-4 days) LC50 assays were finally selected. 50 ), 29 groups of chronic NOECs (minimum exposure time of 4 days [covering sensitive life stages) DO HOEC DO Data, and passed quality control. Among them, the LC of each species... 50 Data range: 0.35–3.26 mg / L; NOEC DO Data range: 0.70–8.40 mg / L; HOEC DO The data range is 0.5~3.0 mg / L.

[0091] S2. Determine the species-average acute value and species-average chronic value for each tested species, and the genus-average acute value and genus-average chronic value for the genus to which each tested species belongs.

[0092] In this embodiment, based on the screened toxicity data, the average acute and chronic toxicity values ​​for each species and genus were calculated according to the methods for calculating acute and chronic toxicity values, and then preprocessed. The nine organisms used to derive the average acute value include *Daphnia zoea*, *Daphnia macrocarpa*, Australian lobster, amphipods, amphipod gnats, grass carp, black carp, silver carp, and the migratory fish *Lycodon grandiflorus* (belonging to 8 genera); LC values ​​for each species were calculated. 50 As ATV, the calculated ranges for 9 SMAVs were 0.36–1.60 mg / L, and for 8 GMAVs were 0.36–1.60 mg / L. The nine organisms used to derive the mean chronic values ​​included *Eupolyphaga sinensis*, *Daphnia zonata*, *Daphnia macrocarpa*, *Chironomida heteromorpha*, *Chironomida extensi*, *Amphipoda*, *Rare gudgeon*, *Silver carp*, and *Striped wolf perch* (belonging to 8 genera); NOECs for each species were used. DO HOEC DO The calculated CTV ranged from 0.60 to 3.29 mg / L, the calculated SMCV ranges for 9 items ranged from 0.60 to 3.29 mg / L, and the calculated GMCV ranges for 8 items ranged from 0.60 to 3.29 mg / L.

[0093] S3. Arrange the average acute value and average chronic value of genera in descending order of toxicity, and assign ranks to the genera corresponding to the average acute value and the genera corresponding to the average chronic value of genera. When ranking the genus-average acute values, the final acute value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average acute value; when ranking the genus-average chronic values, the final chronic value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average chronic value.

[0094] In this embodiment, the cumulative frequency is calculated and a cumulative frequency distribution map is plotted (see...). Figure 2 and Figure 3 Based on the toxicity percentage ranking method, the four genera closest to the 95% cumulative frequency were selected as the most sensitive genera for acute toxicity data (the species involved include amphipods, grass carp, amphipods, and striped wolf bass, with GMAVs of 0.70, 0.83, 1.49, and 1.60 mg / L, respectively, P...). 1R The values ​​were 0.56, 0.67, 0.78, and 0.89, respectively. The values ​​were 0.75, 0.82, 0.88, and 0.94, respectively, with corresponding lnGMAV values ​​of -0.36, -0.19, 0.40, and 0.47. 2 The values ​​were 0.13, 0.03, 0.16 and 0.22 respectively, and the final acute value was calculated to be 2.03 mg / L using the relevant formula.

[0095] Similarly, the genera most sensitive to chronic toxicity data were selected (species including striped wolf bass, amphipod, rare gudgeon, and silver carp, with GMCVs of 1.90, 1.99, 2.96, and 3.29 mg / L, respectively). 2R The values ​​were 0.56, 0.67, 0.78, and 0.89, respectively. The values ​​are 0.75, 0.82, 0.88, and 0.94, respectively, with corresponding lnGMCV values ​​of 0.64, 0.69, 1.09, and 1.19. (lnGMCV) 2 The values ​​were 0.41, 0.47, 1.18 and 1.42 respectively. The final chronic value was calculated to be 3.74 mg / L using the same steps as above.

[0096] S4. Determine the acute dissolved oxygen baseline concentration and the chronic dissolved oxygen baseline concentration based on the obtained final acute value and final chronic value, respectively; determine the acute dissolved oxygen baseline saturation based on the acute dissolved oxygen baseline concentration and the saturated dissolved oxygen concentration, and determine the chronic dissolved oxygen baseline saturation based on the chronic dissolved oxygen baseline concentration and the saturated dissolved oxygen concentration.

[0097] In this embodiment, due to insufficient LC5 data for freshwater organisms, the LC5 (1.95 mg / L) and LC5 of the striped wolf perch (euryhaline fish) were used. 50The ratio of 1.23 (1.58 mg / L) was corrected by multiplying the final acute value to obtain an acute baseline concentration of 2.50 mg / L; the final chronic value did not need to be corrected, i.e., the chronic baseline concentration was 3.74 mg / L.

[0098] Since the relevant toxicity data comes from aquatic species widely distributed in the Yangtze and Yellow River basins, a temperature-water temperature prediction model (corrected R²) is established based on the monthly average water temperature monitoring data of national control sections in the Yangtze and Yellow River basins from 2020 to 2022 released by the National Environmental Monitoring Network and the monthly average air temperature data of CN05.1 (https: / / www.climatechange-data.cn / resource / detail?id=228). 2 =0.879, p-value <0.001):

[0099] In the formula, T w Water temperature, in °C; T a The temperature is expressed in °C. This is used to simulate the water temperature in the Yangtze and Yellow River basins from 1980 to 2022.

[0100] Based on the monthly average temperature data of CN05.1 from 1980 to 2022 and the elevation data (spatial resolution 0.25°) used to generate the CN05.1 dataset, and according to the air temperature-water temperature prediction model, as well as the calculation formula for saturated dissolved oxygen concentration in rivers and low-salinity lakes and reservoirs, combined with Henry's Law and pressure correction, the saturated dissolved oxygen concentration in the Yangtze and Yellow River basins was calculated to be 8.30 mg / L. The percentage ratios of acute and chronic dissolved oxygen baseline concentrations to saturated dissolved oxygen concentrations were then calculated, yielding acute and chronic dissolved oxygen baseline saturations of 30.0% and 45.0%, respectively.

[0101] The specific data for the above embodiments are as follows.

[0102] Table 1 Acute toxicity data of dissolved oxygen in freshwater organisms

[0103] Table 2 Chronic toxicity data of dissolved oxygen in freshwater organisms

[0104] Note: S, G, and R represent the effects of a certain DO concentration on the survival, growth, and reproduction of organisms, respectively.

[0105] Perform toxicity data preprocessing, including calculation of acute and chronic toxicity values.

[0106] In this embodiment, based on the screened toxicity data, the average acute and chronic toxicity values ​​for each species and genus were calculated according to the calculation methods for acute and chronic toxicity values, and then preprocessed. The nine organisms used to derive the average acute value include *Daphnia zoea*, *Daphnia macrocarpa*, Australian lobster, amphipods, amphipod gnats, grass carp, black carp, silver carp, and the migratory fish *Lycodon grandiflorus* (belonging to 8 genera; relevant data are shown in Table 3). Taking *Daphnia zoea* and *Daphnia macrocarpa* as examples, using LC... 50 As an ATV, the following is calculated:

[0107]

[0108]

[0109] The nine organisms used to derive the average chronic value include *Eupolyphaga sinensis*, *Daphnia zonata*, *Daphnia macrocarpa*, *Chironomida heteromorpha*, *Chironomida extensa*, amphipods, rare gudgeon, silver carp, and striped wolf perch (belonging to 8 genera; relevant data are shown in Table 4). Taking silver carp (of the genus *Cetacea*) as an example, the specific calculation is basically the same as the above process:

[0110]

[0111]

[0112] Table 3. Calculation results of acute toxicity values ​​of dissolved oxygen in freshwater organisms (mg / L)

[0113] Table 4. Calculation results of chronic DO toxicity values ​​(mg / L) for freshwater organisms

[0114] In this embodiment, the cumulative frequency was calculated and a cumulative frequency distribution map was plotted. Based on the toxicity percentage ranking method, the four genera closest to the 95% cumulative frequency were selected as the most sensitive genera for acute toxicity data (the species involved include amphipods, grass carp, amphipods, and striped wolf bass, with GMAVs of 0.70, 0.83, 1.49, and 1.60 mg / L, respectively). 1R The values ​​were 0.56, 0.67, 0.78, and 0.89, respectively. The values ​​were 0.75, 0.82, 0.88, and 0.94, respectively, with corresponding lnGMAV values ​​of -0.36, -0.19, 0.40, and 0.47. 2The values ​​were 0.13, 0.03, 0.16, and 0.22 respectively. The final acute value was calculated to be 2.03 mg / L using the following formula (inconsistencies between the listed and actual values ​​are due to differences in the number of decimal places retained between the listed and original data):

[0115]

[0116]

[0117]

[0118]

[0119]

[0120]

[0121]

[0122]

[0123]

[0124] Similarly, the genera most sensitive to chronic toxicity data were selected (species including striped wolf bass, amphipod, rare gudgeon, and silver carp, with GMCVs of 1.90, 1.99, 2.96, and 3.29 mg / L, respectively, P). 2R The values ​​were 0.56, 0.67, 0.78, and 0.89, respectively. The values ​​are 0.75, 0.82, 0.88, and 0.94, respectively, with corresponding lnGMCV values ​​of 0.64, 0.69, 1.09, and 1.19. (lnGMCV) 2 The values ​​were 0.41, 0.47, 1.18 and 1.42 respectively. The final chronic value was calculated to be 3.74 mg / L using the same steps as above.

[0125] In this embodiment, due to insufficient LC5 data for freshwater organisms, the LC5 (1.95 mg / L) and LC5 of the striped wolf perch (euryhaline fish) were used. 50 The ratio of 1.23 (1.58 mg / L) was corrected by multiplying the final acute value to obtain an acute concentration of 2.50 mg / L; the final chronic value did not need to be corrected, i.e., the chronic concentration was 3.74 mg / L.

[0126] This invention discloses an apparatus for determining a dissolved oxygen baseline value in aquatic environments, the apparatus comprising: Data acquisition module: Configured to identify test species in the aquatic environment, which reflect the characteristics of aquatic flora and represent different ecological trophic levels; acquire integrated environmental-aquatic organism toxicity data, which includes toxicity data and environmental data; and determine the species and genus corresponding to the test species. Acute and chronic value calculation module: configured to determine the species average acute value, species average chronic value, genus average acute value, and genus average chronic value of each tested species. Sorting module: Configured to sort the average acute value and average chronic value of genera in descending order of toxicity, and assign ranks to the genera corresponding to the average acute value and the genera corresponding to the average chronic value of genera after sorting. When ranking the genus-average acute values, the final acute value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average acute value; when ranking the genus-average chronic values, the final chronic value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average chronic value. Dissolved oxygen baseline concentration determination module: configured to determine acute dissolved oxygen baseline concentration and chronic dissolved oxygen baseline concentration based on the obtained final acute value and final chronic value respectively; determine acute dissolved oxygen baseline saturation based on acute dissolved oxygen baseline concentration and saturated dissolved oxygen concentration; and determine chronic dissolved oxygen baseline saturation based on chronic dissolved oxygen baseline concentration and saturated dissolved oxygen concentration.

[0127] The present invention further provides an electronic device, comprising: A processor is used to execute multiple instructions; Memory, used to store multiple instructions; The plurality of instructions are to be stored in the memory and loaded and executed by the processor as described above.

[0128] The present invention further provides a computer-readable storage medium storing a plurality of instructions; the plurality of instructions are used by a processor to load and execute the method as described above.

[0129] It should be noted that, unless otherwise specified, the embodiments and features described in the present invention can be combined with each other.

[0130] In the embodiments provided by this invention, it should be understood that the disclosed systems, apparatuses, and methods can be implemented in other ways. For example, the apparatus embodiments described above are merely illustrative; for instance, the division of units is only a logical functional division, and in actual implementation, there may be other division methods. For example, multiple units or components may be combined or integrated into another system, or some features may be ignored or not executed. Furthermore, the coupling or direct coupling or communication connection shown or discussed may be an indirect coupling or communication connection between devices or units through some interfaces, and may be electrical, mechanical, or other forms.

[0131] The units described as separate components may or may not be physically separate. The components shown as units may or may not be physical units; that is, they may be located in one place or distributed across multiple network units. Some or all of the units can be selected to achieve the purpose of this embodiment according to actual needs.

[0132] Furthermore, the functional units in the various embodiments of the present invention can be integrated into one processing unit, or each unit can exist physically separately, or two or more units can be integrated into one unit. The integrated unit can be implemented in hardware or in the form of hardware plus software functional units.

[0133] The integrated unit, implemented as a software functional unit, can be stored in a computer-readable storage medium. This software functional unit, stored in a storage medium, includes several instructions to cause a computer device (which may be a personal computer, a physical server, or a cloud server, etc., requiring the Ubuntu operating system to be installed) to execute some steps of the methods described in the various embodiments of this invention. The aforementioned storage medium includes various media capable of storing program code, such as a USB flash drive, a portable hard drive, a read-only memory (ROM), a random access memory (RAM), a magnetic disk, or an optical disk.

[0134] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Any simple modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present invention shall still fall within the scope of the technical solution of the present invention.

Claims

1. A method for determining a baseline value of dissolved oxygen in aquatic environments, characterized in that the method... include: Step S1: Identify the test species in the aquatic environment. The test species reflect the characteristics of the aquatic flora and represent different ecological trophic levels. Obtain comprehensive environmental-aquatic organism toxicity data, which includes toxicity data and environmental data. Determine the species and genus corresponding to the test species. Step S2: Determine the species mean acute value and species mean chronic value for each tested species, and the genus mean acute value and genus mean chronic value for the genus to which each tested species belongs. Step S3: Sort the average acute value and average chronic value of the genera in descending order of toxicity, and assign ranks to the genera corresponding to the average acute value and the genera corresponding to the average chronic value of the genera. When ranking the genus-average acute values, the final acute value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average acute value; when ranking the genus-average chronic values, the final chronic value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average chronic value. Step S4: Determine the acute dissolved oxygen baseline concentration and the chronic dissolved oxygen baseline concentration based on the obtained final acute value and final chronic value, respectively; The acute dissolved oxygen baseline saturation is determined based on the acute dissolved oxygen baseline concentration and the saturated dissolved oxygen concentration, and the chronic dissolved oxygen baseline saturation is determined based on the chronic dissolved oxygen baseline concentration and the saturated dissolved oxygen concentration.

2. The method as described in claim 1, characterized in that, In step S1, the test species include at least 8 species and cover the following biological groups: bony fish of the Cyprinidae family, bony fish of non-cyprinidae family, and benthic or planktonic crustaceans.

3. The method as described in claim 1, characterized in that, Step S2: Determine the species-average acute and chronic values ​​for each tested species, and the genus-average acute and chronic values ​​for the genus to which each tested species belongs, wherein: The formula for calculating the species mean acute value of the tested species is: ,in, Let be the species-average acute toxicity value corresponding to the i-th tested species, k represent the acute toxicity effect category (dimensionless), and m represent the number of acute toxicity values ​​for species i. This represents the p-th acute toxicity value of species i, expressed in mg / L. ; The formula for calculating the genus-average acute value of the tested species is: Where j is the genus number of the tested species. Let be the genus average acute value for the j-th genus, and 'a' be the number of species included in the j-th genus. Let be the species-average acute value corresponding to the q-th species of the j-th genus. ; The formula for calculating the chronic toxicity value of the test species is as follows: ,in, denoted as the chronic toxicity value for the i-th tested species, and z represents the category of chronic toxicity effect, which is dimensionless. The highest observed concentration of dissolved oxygen for the i-th tested species is given in mg / L. This represents the lowest concentration of dissolved oxygen for the i-th test species with no observable effect, expressed in mg / L. The formula for calculating the species mean chronicity value of the tested species is: ,in, is the species-average chronicity value corresponding to the i-th tested species; n represents the number of chronicity toxicity values ​​for species i. This represents the p1th chronic toxicity value of species i, expressed in mg / L. ; The formula for calculating the genus-average chronicity value of the tested species is as follows: ,in, Let be the genus average chronicity value for the j-th genus. Let be the species-average chronicity value corresponding to the q-th species of the j-th genus. .

4. The method as described in claim 2, characterized in that, In step S3, when ranking the genus-average acute values, the final acute value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average acute value; when ranking the genus-average chronic values, the final chronic value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average chronic value, including: Step S31: When ranking the genus-average acute values, calculate the cumulative acute frequency of each tested species within its genus: ,in, R represents the acute cumulative frequency of the genus to which the tested species belongs, R represents the rank of the genus to which the tested species belongs, and N represents the total number of all genera. The genera containing the four tested species with the smallest distance at the 95% acute cumulative frequency were selected as the first candidate genera, and the acute cumulative frequency of the first candidate genera and the genus average acute value of the genera containing the first candidate genera were retained. Step S32: Calculate the final acute value based on the first candidate genus. : , , , and These are all intermediate quantities obtained from calculations. This is the average acute value; Step S33: When ranking the genus-average chronicity values, calculate the cumulative frequency of chronicity for each tested species within its genus: ,in, The chronic cumulative frequency of the genus to which the tested species belong; The genera containing the four tested species with the smallest distance at the 95% chronic accumulation frequency were selected as second candidate genera. The chronic accumulation frequency of the second candidate genera and the genus average chronic value of the genera containing the second candidate genera were retained. Step S34: Calculate the final chronic value based on the second candidate genus. : , , , and Both 2 are intermediate quantities obtained from calculation. This is the average chronic value.

5. The method as described in claim 4, characterized in that, Step S4: Determine the acute dissolved oxygen baseline concentration and the chronic dissolved oxygen baseline concentration based on the obtained final acute value and final chronic value, respectively; Acute dissolved oxygen baseline saturation is determined based on acute dissolved oxygen baseline concentration and saturated dissolved oxygen concentration, and chronic dissolved oxygen baseline saturation is determined based on chronic dissolved oxygen baseline concentration and saturated dissolved oxygen concentration, including: For the final acute value, Multiply by a correction factor to obtain the acute dissolved oxygen baseline concentration; where the correction factor is the LC5 / LC5 ratio of all tested species. 50 Mean; LC5 is the 5% lethal concentration, LC 50 This is the median lethal concentration; For the final chronic value, As a baseline concentration for chronic dissolved oxygen; The acute dissolved oxygen baseline saturation is determined based on the acute dissolved oxygen baseline concentration and the saturated dissolved oxygen concentration, and the chronic dissolved oxygen baseline saturation is determined based on the chronic dissolved oxygen baseline concentration and the saturated dissolved oxygen concentration.

6. A device for determining the baseline value of dissolved oxygen in aquatic environments, characterized in that, The device includes: Data acquisition module: Configured to identify test species in the aquatic environment, which reflect the characteristics of aquatic flora and represent different ecological trophic levels; acquire integrated environmental-aquatic organism toxicity data, which includes toxicity data and environmental data; and determine the species and genus corresponding to the test species. Acute and chronic value calculation module: configured to determine the species average acute value, species average chronic value, genus average acute value, and genus average chronic value of each tested species. Sorting module: Configured to sort the average acute value and average chronic value of genera in descending order of toxicity, and assign ranks to the genera corresponding to the average acute value and the genera corresponding to the average chronic value of genera after sorting. When ranking the genus-average acute values, the final acute value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average acute value; when ranking the genus-average chronic values, the final chronic value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average chronic value. Dissolved oxygen baseline concentration determination module: configured to determine acute dissolved oxygen baseline concentration and chronic dissolved oxygen baseline concentration based on the obtained final acute value and final chronic value, respectively; The acute dissolved oxygen baseline saturation is determined based on the acute dissolved oxygen baseline concentration and the saturated dissolved oxygen concentration, and the chronic dissolved oxygen baseline saturation is determined based on the chronic dissolved oxygen baseline concentration and the saturated dissolved oxygen concentration.

7. The apparatus as claimed in claim 6, characterized in that, The data acquisition module includes at least eight species and covers the following biological groups: cyprinid fish, non-cyprinid fish, and benthic or planktonic crustaceans.

8. The apparatus as claimed in claim 6, characterized in that, The determination of the species-average acute and chronic values ​​for each tested species, and the genus-average acute and chronic values ​​for the genus to which each tested species belongs, wherein: The formula for calculating the species mean acute value of the tested species is: ,in, Let be the species-average acute toxicity value corresponding to the i-th tested species, k represent the acute toxicity effect category (dimensionless), and m represent the number of acute toxicity values ​​for species i. This represents the p-th acute toxicity value of species i, expressed in mg / L. ; The formula for calculating the genus-average acute value of the tested species is: Where j is the genus number of the tested species. Let be the genus average acute value for the j-th genus, and 'a' be the number of species included in the j-th genus. Let be the species-average acute value corresponding to the q-th species of the j-th genus. ; The formula for calculating the chronic toxicity value of the test species is as follows: ,in, denoted as the chronic toxicity value for the i-th tested species, and z represents the category of chronic toxicity effect, which is dimensionless. The highest observed concentration of dissolved oxygen for the i-th tested species is given in mg / L. This represents the lowest concentration of dissolved oxygen for the i-th test species with no observable effect, expressed in mg / L. The formula for calculating the species mean chronicity value of the tested species is: ,in, is the species-average chronicity value corresponding to the i-th tested species; n represents the number of chronicity toxicity values ​​for species i. This represents the p1th chronic toxicity value of species i, expressed in mg / L. ; The formula for calculating the genus-average chronicity value of the tested species is as follows: ,in, Let be the genus average chronicity value for the j-th genus. Let be the species-average chronicity value corresponding to the q-th species of the j-th genus. .

9. The apparatus as claimed in claim 8, characterized in that, When ranking genus-average acute values, the final acute value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average acute value; when ranking genus-average chronic values, the final chronic value of all tested species is determined based on the rank of the genus to which each tested species belongs and the genus-average chronic value, including: When ranking the genus-average acute values, the cumulative frequency of acute values ​​for each tested species within its genus is calculated: ,in, R represents the acute cumulative frequency of the genus to which the tested species belongs, R represents the rank of the genus to which the tested species belongs, and N represents the total number of all genera. The genera containing the four tested species with the smallest distance at the 95% acute cumulative frequency were selected as the first candidate genera, and the acute cumulative frequency of the first candidate genera and the genus average acute value of the genera containing the first candidate genera were retained. The final acute value was calculated based on the first candidate genus. : , , , and These are all intermediate quantities obtained from calculations. This is the average acute value; When ranking the genus-average chronicity values, the cumulative frequency of chronicity for each tested species within its genus is calculated: ,in, The chronic cumulative frequency of the genus to which the tested species belong; The genera containing the four tested species with the smallest distance at the 95% chronic accumulation frequency were selected as second candidate genera. The chronic accumulation frequency of the second candidate genera and the genus average chronic value of the genera containing the second candidate genera were retained. The final chronicity value was calculated based on the second candidate genus. : , , , and Both 2 are intermediate quantities obtained from calculation. This is the average chronic value.

10. The apparatus as claimed in claim 9, characterized in that, The acute dissolved oxygen baseline concentration and the chronic dissolved oxygen baseline concentration are determined based on the obtained final acute value and final chronic value, respectively. Acute dissolved oxygen baseline saturation is determined based on acute dissolved oxygen baseline concentration and saturated dissolved oxygen concentration, and chronic dissolved oxygen baseline saturation is determined based on chronic dissolved oxygen baseline concentration and saturated dissolved oxygen concentration, including: For the final acute value, Multiply by a correction factor to obtain the acute dissolved oxygen baseline concentration; where the correction factor is the LC5 / LC5 ratio of all tested species. 50 Mean; LC5 is the 5% lethal concentration, LC 50 This is the median lethal concentration; For the final chronic value, As a baseline concentration for chronic dissolved oxygen; The acute dissolved oxygen baseline saturation is determined based on the acute dissolved oxygen baseline concentration and the saturated dissolved oxygen concentration, and the chronic dissolved oxygen baseline saturation is determined based on the chronic dissolved oxygen baseline concentration and the saturated dissolved oxygen concentration.

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