Process method for extracting nucleic acid

Abstract

The invention provides a process method for extracting nucleic acid, which comprises a splitting area, a magnetic bead area, a rinsing area and a collecting area which are combined into a whole, and the splitting area is used for splitting a sample to be detected; the magnetic bead area is used for storing magnetic beads; the rinsing area is used for rinsing nucleic acid to be detected; the collecting area is used for collecting to-be-detected nucleic acid. The magnetic rod sleeve is controlled by the magnetic rod to sequentially enter the magnetic bead area, the cracking area, the rinsing area and the collecting area of the kit, so that the processes of magnetic bead adsorption, sample cracking, nucleic acid rinsing and elution collection are completed. The kit is simple in structure, high in integration level, capable of being used for nucleic acid extraction of one sample and position arrangement and placement of all the areas, capable of giving consideration to operation convenience, capable of keeping balance during placement, simple in structure, small in size, light in weight and suitable for mass production and transportation.

Description

Technical Field

[0001] The present invention belongs to the technical field of nucleic acid extraction, and particularly relates to a process method for nucleic acid extraction. Background Art

[0002] The nucleic acid extraction technology in molecular biology research has undergone a remarkable evolution process. Traditional manual operation methods are not only inefficient, but the experimental results are also easily affected by the technical differences of operators. The introduction of modern industrial automation technology has completely changed this situation - through the high repeatability of automated equipment, the reliability and operation efficiency of nucleic acid extraction have been significantly improved.

[0003] As a highly integrated automated instrument, the nucleic acid extractor realizes the fully enclosed automated operation of the nucleic acid extraction process. This integrated design not only greatly shortens the operation time and reduces human error, but also meets the portability requirements of primary medical care and on-site detection through the miniaturization design of the equipment, becoming an important research and development direction in this field.

[0004] Traditional nucleic acid extraction mainly relies on manual operation. Its typical process includes: cell lysis, separation of nucleic acid from impurities (such as proteins and cell debris), rinsing and purification of nucleic acid, and finally elution and collection. Although the manual method has a lower cost, it has significant limitations: First, the operation steps are cumbersome and inefficient, making it difficult to meet the requirements of high-throughput sample detection; second, the operation results highly depend on the proficiency of personnel, with poor repeatability and easy introduction of human error; third, during the operation process, the sample is easily exposed to the environment, posing a risk of aerosol contamination, which affects the specificity and sensitivity of detection.

[0005] In order to overcome the drawbacks of manual operation, automated nucleic acid extraction technology has emerged. The automated nucleic acid extractor realizes the enclosed automation of the nucleic acid extraction process through robotic arms, liquid path systems, and program control, greatly improving the standardization degree, throughput, and repeatability of operation, while reducing the biosafety risk.

[0006] However, the efficient operation of the automated nucleic acid extractor highly depends on its dedicated nucleic acid extraction kit. Currently, there are still many areas to be improved in the kit and its usage method that are配套 with automated equipment:

[0007] 1. Insufficient process integration and operation logic: The process design of some automated extraction methods is not optimized enough, resulting in complex and redundant movement paths of robotic arms or magnetic rod sleeves, and many redundant steps, which prolongs the total extraction time. If the transfer logic of magnetic beads in different functional intervals such as lysis, rinsing, and elution is not carefully designed, the extraction efficiency will be reduced.

[0008] 2. Stability and Sealing Issues: During automation, the reagent kit needs to withstand mechanical movement, liquid transfer, and temperature changes. If the kit itself is not placed stably or properly sealed within the instrument, liquid spillage, cross-contamination, or reagent evaporation may occur, directly affecting extraction efficiency and nucleic acid quality. Existing methods do not pay sufficient attention to the dynamic stability of the reagent kit within the instrument and the effective sealing during high-temperature steps.

[0009] 3. Method Flexibility and Purity Bottlenecks: For some difficult-to-lyse samples or downstream applications with extremely high requirements for nucleic acid purity, the lysis efficiency and rinsing effect of existing automated methods may become bottlenecks. Fixed, unoptimized rinsing times and mixing methods may not completely remove inhibitors, affecting subsequent analyses.

[0010] 4. Dependence on Consumables: The core of automated methods lies in the synergy of "instruments, reagent kits, and processes." An excellent extraction process must be closely integrated with a reagent kit of a specific structure (such as the spatial layout of each zone, the location of the heating zone, and the sealing method). Existing technologies often fail to optimize the physical structure of the reagent kit and the operational steps of the extraction method from a system-level perspective, resulting in suboptimal overall performance.

[0011] Therefore, there is an urgent need in this field for a nucleic acid extraction method that is not only highly automated but also highly optimized in its workflow and closely integrated with specialized reagent kits. This method should feature simple steps, a rational pathway, high stability, good sealing, high extraction purity, and applicability to multiple sample types, in order to better meet the requirements of modern molecular detection for efficiency, accuracy, and reliability. Summary of the Invention

[0012] The present invention proposes a nucleic acid extraction process method that solves the above-mentioned problems existing in the prior art.

[0013] To solve the above-mentioned technical problems, the present invention is achieved through the following technical solution:

[0014] A method for nucleic acid extraction, characterized by comprising a lysis zone, a magnetic bead zone, a rinsing zone, and a collection zone integrated into one unit; the lysis zone is used for lysis of the sample to be tested; the magnetic bead zone is used for magnetic bead preservation; the rinsing zone is used for rinsing the nucleic acid to be tested; and the collection zone is used for collecting the nucleic acid to be tested; the following steps are performed during automated nucleic acid extraction:

[0015] Place the reagent kit in the automated nucleic acid extractor and fix it in place;

[0016] A magnetic rod sleeve is placed on the magnetic rod;

[0017] The preset nucleic acid extraction program is started, the instrument performs a self-test and begins heating the lysis zone;

[0018] The magnetic rods are used to control the magnetic rod sleeves to sequentially enter the magnetic bead area, lysis area, rinsing area and collection area of ​​the kit, completing the magnetic bead adsorption, sample lysis, nucleic acid rinsing and elution collection process.

[0019] Preferably, the magnetic bead adsorption step includes:

[0020] The magnetic rod drives the magnetic rod sleeve into the liquid surface of the magnetic bead zone;

[0021] Magnetic beads are attracted to the magnetic rod sleeve by magnetic force;

[0022] Raise the magnetic rod and magnetic rod sleeve to above the liquid surface.

[0023] Preferably, the sample lysis step includes:

[0024] Move the magnetic rod sleeve with adsorbed magnetic beads to the liquid surface in the pyrolysis zone;

[0025] The magnetic rod sleeve remains in the pyrolysis solution for heating and pyrolysis;

[0026] After the pyrolysis is complete, the magnetic rod attracts the magnetic beads again and lifts them above the liquid surface.

[0027] Preferably, the nucleic acid washing step includes:

[0028] The magnetic rod sleeves with adsorbed magnetic beads are sequentially moved into multiple rinsing solution tanks in the rinsing area;

[0029] The washing process is performed in each rinsing tank, including magnetic bead release, mixing, and re-adsorption.

[0030] After the final rinse, the magnetic beads are dried in the rinsing zone.

[0031] Preferably, the elution and collection step includes:

[0032] The dried magnetic beads are then transferred to the eluent tank in the collection area.

[0033] Elution is performed under heating conditions to dissolve the nucleic acids in the eluent;

[0034] After elution is complete, the magnetic rod attracts the magnetic beads and removes them from the collection area.

[0035] Preferably, the magnetic rod sleeve is for single use, and the adsorption and release of the magnetic beads are controlled by magnetic force each time it enters and leaves the liquid surface.

[0036] Preferably, both the pyrolysis zone and the collection zone are equipped with heating modules during operation to control the reaction temperature.

[0037] Preferably, the rinsing area is provided with at least three rinsing tanks for multiple rinsings to improve nucleic acid purity.

[0038] Preferably, the reagent kit is a disposable consumable, and its position and layout in the automated nucleic acid extractor are optimized to ensure the stability of the reagent kit and the convenience of operation during the process.

[0039] Preferably, the method is applicable to nucleic acid extraction from environmental samples, clinical samples, or biological samples.

[0040] The present invention has the following beneficial effects:

[0041] 1. The placement and arrangement of each section within the reagent kit should not only ensure ease of operation and prevent errors, but also maintain the balance of the reagent kit when placed individually, preventing it from tipping over and thus preventing reagent bottles or tools from falling out or becoming misplaced.

[0042] 2. It has a simple structure, small size and light weight, making it suitable for mass production and transportation. Attached Figure Description

[0043] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0044] Figure 1 A schematic diagram of the nucleic acid extraction kit provided in Example 1;

[0045] Figure 2 An exploded view of the nucleic acid extraction kit provided in Example 1;

[0046] Figure 3 An exploded view (viewed from a lower angle) of the nucleic acid extraction kit provided in Example 1;

[0047] Figure 4 A top view of the nucleic acid extraction kit provided in Example 1;

[0048] Figure 5 A front view of the nucleic acid extraction kit provided in Example 1;

[0049] Figure 6 Rear view of the nucleic acid extraction kit provided in Example 1;

[0050] Figure 7 Left view of the nucleic acid extraction kit provided in Example 1;

[0051] Figure 8 Right view of the nucleic acid extraction kit provided in Example 1;

[0052] Figure 9A bottom view of the nucleic acid extraction kit provided in Example 1;

[0053] Figure 10 This is a schematic diagram of the magnetic rod sleeve provided in Example 1. Detailed Implementation

[0054] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0055] Please see Figure 1-10 As shown, the present invention is an automated nucleic acid extraction kit, an acid extraction kit, comprising a lysis zone, a magnetic bead zone, a rinsing zone, and a collection zone, wherein the lysis zone, magnetic bead zone, rinsing zone, and collection zone are integrated into a single structure; the lysis zone is used for lysis of the sample to be tested; the magnetic bead zone is used for magnetic bead preservation; the rinsing zone is used for rinsing the nucleic acid to be tested; and the collection zone is used for collecting the nucleic acid to be tested.

[0056] Furthermore, the upper surface of the reagent kit is arranged in a horizontal rectangle, and the space below the reagent kit is set according to the actual needs of each area.

[0057] In some designs, the space below the reagent kit is set according to the actual needs of each area, presenting a state where the height is the same but the width is different. The magnetic bead area, rinsing area and collection area are the same, while the lysis area is wider than the other areas, which can make the reagent kit stand stably.

[0058] Furthermore, each zone provides space for the lysis of the sample to be tested and the collection of nucleic acid. The lysis zone is located near the left wide side of the rectangular surface of the kit and is designated as hole 1, which is a rectangular opening. The magnetic bead zone is located near the lysis zone and is designated as hole 2, which is a circular opening. The rinsing zone is located near the magnetic bead zone and is designated as holes 3-5, which are circular openings. The collection zone is separated from the rinsing zone by one hole and is designated as hole 7, which is a circular opening. The four zones are arranged in parallel and are all upward-opening grooves.

[0059] Furthermore, the opening on the upper surface of the reagent kit is covered with an aluminum film.

[0060] Since the reagent kit contains various reaction reagents pre-stored, it must be strictly sealed, and silicone gaskets cannot be used for sealing, in order to ensure a better sealing effect.

[0061] Furthermore, the reagent area includes a first reagent area, a second reagent area, a third reagent area, and a fourth reagent area; the first reagent area is located on the left wide side of the reagent kit and contains lysis buffer reagent; the second reagent area is located on the left-middle position of the upper rectangular surface of the reagent kit and contains magnetic bead solution reagent; the third reagent area is located in the middle position of the upper rectangular surface of the reagent kit and contains one part of wash solution 1 and two parts of wash solution 2; the fourth reagent area is located on the right wide side of the upper rectangular surface of the reagent kit and contains elution buffer reagent.

[0062] Nucleic acid extraction kits are typically single-use consumables that require mass production and transportation. They must be small and lightweight, while maintaining balance when placed individually to prevent tipping. They must be designed to prevent falling or misalignment during placement, be easily accessible during use without jamming, and maintain overall balance to prevent tipping.

[0063] Therefore, the placement of each section within the reagent kit requires comprehensive consideration of various factors and must be determined through rigorous testing and screening. The placement of each reagent within the reagent section has been selected after numerous repeated experiments to best simplify pipetting procedures, minimize errors, and maintain the overall balance of the kit, preventing tipping. Furthermore, the reagent section must be divided into four reagent zones, each placed in a different location within the kit. This ensures better balance while maintaining ease of operation and preventing errors.

[0064] For example, the lysis buffer needs to be placed in the first reagent area, and the eluent reagent needs to be placed in the fourth reagent area. The main reason is that both the lysis buffer and the eluent need to be heated during use.

[0065] In some embodiments, the nucleic acid extraction kit provided by the present invention can be manufactured as a low-cost disposable consumable or as a device that can be repeatedly sterilized, either wholly or partially.

[0066] Example 1: Nucleic acid extraction kit provided by the present invention

[0067] The nucleic acid extraction kit provided in this embodiment is as follows: Figures 1-10 As shown, Figure 1 This is a schematic diagram of the overall structure of the nucleic acid extraction kit. Figure 2 An exploded view of a nucleic acid extraction kit; Figure 3 An exploded view of the nucleic acid extraction kit (viewed from a lower angle); Figure 4 Top view of the nucleic acid extraction kit; Figure 5 This is a front view of the nucleic acid extraction kit; Figure 6 This is a rear view of the nucleic acid extraction kit; Figure 7Left view of the nucleic acid extraction kit; Figure 8 Right view of the nucleic acid extraction kit; Figure 9 This is a bottom view of the nucleic acid extraction kit; Figure 10 This is a schematic diagram of the magnetic rod sleeve.

[0068] The nucleic acid extraction kit 1 (hereinafter referred to as kit 1) provided in this embodiment needs to be installed in an automated nucleic acid extractor to complete the extraction and detection of nucleic acid.

[0069] like Figures 1-3 As shown, kit 1 includes a lysis zone, a magnetic bead zone, a rinsing zone, and a collection zone; the lysis zone is used for lysis of the sample to be tested; the magnetic bead zone is used for magnetic bead preservation; the rinsing zone is used for rinsing the nucleic acid to be tested; and the collection zone is used for collecting the nucleic acid to be tested.

[0070] like Figure 4 and 9 As can be seen, the upper surface of reagent kit 1 is arranged in a rectangular, horizontal pattern. (For example...) Figure 4 and 5 Reagent Kit 1

[0071] The bottom is set according to the actual space required by each area, presenting a state where the height is the same but the width is different. The magnetic bead area, rinsing area and collection area are the same, and the lysis area is wider than the other areas, which can make the reagent kit 1 stand stably vertically.

[0072] like Figures 1-10 The lysis zone 1 is an upward-opening groove that provides space for the lysis of the sample. The bottom of the groove protrudes downwards, forming a space. When the kit 1 is placed inside the automated nucleic acid extractor, the lysis zone is located above the heating module and near the lower end of the magnet. During sample lysis, the magnetic rod is inserted into the lysis zone for mixing. After mixing, it is raised and moved directly above the lysis zone, making it easier to remove the solution from the lysis zone. The lysis zone is positioned near the wide left side of the rectangular surface of the kit.

[0073] like Figures 1-10 The magnetic bead area 2 is an upward-opening tank that provides space for storing the magnetic bead solution. The bottom of the tank protrudes downwards, forming a space. When the reagent kit 1 is placed in the automated nucleic acid extractor, the magnetic bead area is located near the lower end of the magnet inside the extractor. After sample lysis is complete, the magnetic rod is moved to the top of the magnetic bead area. The magnetic attraction of the magnet causes the magnetic beads to gather on the magnetic rod, making it easier to remove the reagents from the lysis zone.

[0074] like Figures 1-10The rinsing zone 3 is an upward-opening tank divided into three slots, providing more convenient and thorough space for rinsing the nucleic acids to be tested. When the reagent kit 1 is placed inside the automated nucleic acid extractor, the rinsing zone is located near the lower end of the magnet inside the automated nucleic acid extractor. The magnetic rod enters the rinsing zone to mix the solution, and after completion, it is raised and moved to the top of the rinsing zone, making it easier to remove the solution in the lysis zone. The rinsing zone is located in the middle of the rectangular surface of the reagent kit.

[0075] like Figures 1-10 Collection area 4 is an upward-facing tank. When reagent kit 1 is placed inside the automated nucleic acid extractor, the collection area is located above the heating module and near the lower right side of the magnet. The magnetic rod is inserted into the collection area for mixing, and then lifted and moved directly above the collection area, making it easier to remove the solution. The collection area is positioned on the right side of the rectangular surface of the reagent kit.

[0076] Since reagents are pre-stored in reagent sections 5-10, they must be strictly sealed; silicone gaskets cannot be used for sealing to ensure a better seal. Figure 4 Reagent zone 5 contains lysis buffer, reagent zone 6 contains magnetic bead solution, reagent zone 7 contains rinsing solution 1, reagent zones 8 and 9 contain rinsing solution 2, and reagent zone 10 contains elution solution. Since the reagent kit is typically a single-use consumable requiring mass production and transportation, it must be small and lightweight, while maintaining balance when placed individually to prevent the reagents in kit 1 from tipping over. The placement of each zone within the kit requires comprehensive consideration and must be determined through rigorous testing and screening. The placement of each reagent bottle 18 in reagent bottle zone 5 was selected after numerous repeated experiments to best simplify the magnetic rod application process, minimize errors, and maintain the overall balance of kit 1. Furthermore, reagent bottle zones 5 and 10 must be divided into two separate zones, placed at opposite ends of kit 1, to better balance ease of operation and prevent errors. For example, the lysis reagent in reagent zone 5 and the elution reagent in reagent bottle 10 need to be placed above the heating module during use. The main reason is that both the lysis buffer and the elution buffer need to be heated during use, and the lysis zone needs to be preheated to 65°C in advance.

[0077] Example 2: Nucleic Acid Extraction Process

[0078] This embodiment uses the nucleic acid extraction kit 1 provided in Example 1 for nucleic acid extraction, including the following steps:

[0079] Place the sample into the automated nucleic acid extractor for fixation. Place a disposable magnetic rod sleeve on the magnetic rod of the automated nucleic acid extractor. Follow the prompts to select the appropriate automated extraction program, click "Start Detection," and the instrument will perform a self-check. The lysis zone will then begin heating.

[0080] The magnetic rod of the automated nucleic acid extractor controls the magnetic rod sleeve to enter the liquid surface of reagent zone 6 in the magnetic bead area. The magnetic attraction of the magnet causes the magnetic beads to gather on the magnetic rod sleeve. Then the magnetic rod and the magnetic rod sleeve with the adsorbed magnetic beads leave the liquid surface of the magnetic bead area (reagent zone 6) and go directly above it.

[0081] The magnetic rod and the magnetic rod sleeve with the magnetic beads adsorbed move to the left and are directly above the reagent area 5 of the pyrolysis zone. Then they move down together and enter the liquid surface of the pyrolysis zone. Subsequently, the magnetic rod sleeve remains in the liquid surface and rises away from the liquid surface. The magnetic beads on the magnetic rod sleeve enter the pyrolysis zone for dissolution by heating.

[0082] After the pyrolysis zone is completely dissolved, the magnetic rod moves down into the magnetic rod sleeve. Through magnetic attraction, the magnetic beads gather on the magnetic rod sleeve. Then, the magnetic rod and the magnetic rod sleeve with the adsorbed magnetic beads leave the liquid surface at position 5 of the reagent zone of the pyrolysis zone and return to the top.

[0083] The magnetic rod and the magnetic rod sleeve with the magnetic beads adsorbed move to the right and are directly above the reagent area 7 in the rinsing zone. Then they move down together and enter the liquid surface. The magnetic rod sleeve remains in the liquid surface, the magnetic rod rises and leaves the liquid surface, and the magnetic beads on the magnetic rod sleeve enter the rinsing zone hole 1 for washing.

[0084] After rinsing in the rinsing zone, the magnetic rod moves down into the magnetic rod sleeve, and the magnetic beads gather on the magnetic rod sleeve through magnetic attraction. Then the magnetic rod and the magnetic rod sleeve with the magnetic beads adsorbed together leave the reagent area 7 position of the rinsing zone and return to the top.

[0085] The magnetic rod and the magnetic rod sleeve with the magnetic beads adsorbed move to the right and are directly above the reagent area 8 in the rinsing zone. Then they move down together and enter the liquid surface. The magnetic rod sleeve remains in the liquid surface, the magnetic rod rises and leaves the liquid surface, and the magnetic beads on the magnetic rod sleeve enter the reagent area 8 in the rinsing zone for washing.

[0086] After rinsing in the rinsing zone, the magnetic rod moves down into the magnetic rod sleeve, and the magnetic beads gather on the magnetic rod sleeve through magnetic attraction. Then the magnetic rod and the magnetic rod sleeve with the magnetic beads adsorbed together leave the reagent area 8 position of the rinsing zone and return to the top.

[0087] The magnetic rod and the magnetic rod sleeve with the magnetic beads adsorbed move to the right and move directly above the reagent area 9 in the rinsing zone, then move down together and enter the liquid surface. Subsequently, the magnetic rod sleeve remains in the liquid surface, the magnetic rod rises and leaves the liquid surface, and the magnetic beads on the magnetic rod sleeve enter the reagent area 9 in the rinsing zone for washing.

[0088] After rinsing in the rinsing zone, the magnetic rod moves down into the magnetic rod sleeve, and the magnetic beads gather on the magnetic rod sleeve through magnetic attraction. Then the magnetic rod and the magnetic rod sleeve with the magnetic beads adsorbed together leave the reagent area 9 position of the rinsing zone and return to the top.

[0089] The magnetic rod and the magnetic rod sleeve with the magnetic beads adsorbed move to the right and are directly above position 9 of the reagent area in the rinsing zone. Then they move down together and enter the hole. The magnetic rod sleeve remains in the hole, the magnetic rod rises and leaves the liquid surface, and the magnetic beads on the magnetic rod sleeve are dried.

[0090] After drying, the magnetic rod moves down into the magnetic rod sleeve, and the magnetic beads are attracted to the magnetic rod sleeve by magnetic attraction. Then the magnetic rod and the magnetic rod sleeve with the magnetic beads adsorbed together leave the reagent area 9 of the rinsing area.

[0091] The magnetic rod and the magnetic rod sleeve with the magnetic beads adsorbed move to the right and are directly above the reagent zone 10 of the collection area, then move down together and enter the hole. The magnetic rod sleeve remains in the hole, the magnetic rod rises and leaves the liquid surface, and the magnetic beads on the magnetic rod sleeve enter the liquid surface at the reagent zone 10 of the collection area for heating and elution.

[0092] After elution, the magnetic rod moves down into the magnetic rod sleeve, where magnetic beads are attracted to the sleeve. Then, the magnetic rod and the sleeve with the beads leave the reagent zone 10 of the collection area and return to the top position. Next, the magnetic rod and the sleeve with the beads move to the left and are directly above the reagent zone 8 of the rinsing area before moving down into the liquid surface. The sleeve remains in the liquid surface, while the magnetic rod rises and leaves the liquid surface. The beads on the sleeve enter the reagent zone 8 of the rinsing area, completing the experiment.

[0093] The nucleic acid extraction method provided in this embodiment achieves a high degree of integration and automation by working in conjunction with a structurally optimized dedicated reagent kit. The movement path of the magnetic rod sleeve is carefully designed with clear logic, avoiding unnecessary movement and improving extraction efficiency. The entire process is completed within a closed reagent kit, effectively preventing cross-contamination and aerosol diffusion. The final obtained nucleic acid has high purity and good integrity, fully meeting the requirements of downstream molecular biology experiments.

[0094] The preferred embodiments of the present invention disclosed above are merely illustrative of the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. A process method for nucleic acid extraction, characterized by, The kit comprises a lysis area, a magnetic bead area, a rinsing area and a collection area which are combined into one; the lysis area is used for lysis treatment of the sample to be tested; the magnetic bead area is used for magnetic bead storage; the rinsing area is used for rinsing the nucleic acid to be tested; the collection area is used for collecting the nucleic acid to be tested; the following steps are performed in the nucleic acid automatic extraction: Place the kit in the nucleic acid automatic extraction instrument and fix it; Put the magnetic rod sleeve on the magnetic rod; Start the preset nucleic acid extraction program, and the instrument self-checks and starts heating the lysis area; Control the magnetic rod sleeve to enter the magnetic bead area, the lysis area, the rinsing area and the collection area of the kit in sequence through the magnetic rod, complete the magnetic bead adsorption, sample lysis, nucleic acid rinsing and elution collection process.

2. The process method of nucleic acid extraction as claimed in claim 1 wherein, The magnetic bead adsorption step includes: Drive the magnetic rod sleeve into the liquid surface of the magnetic bead area by the magnetic rod; Make the magnetic beads gather on the magnetic rod sleeve by magnetic adsorption; Lift the magnetic rod and the magnetic rod sleeve above the liquid surface.

3. The process method of nucleic acid extraction as claimed in claim 1 wherein, The sample lysis step includes: Move the magnetic rod sleeve with adsorbed magnetic beads to the liquid surface of the lysis area; Stop the magnetic rod sleeve in the lysis liquid for heating lysis; After lysis is completed, the magnetic rod adsorbs the magnetic beads again and lifts them above the liquid surface.

4. The process method of nucleic acid extraction as claimed in claim 1 wherein, The nucleic acid rinsing step includes: Move the magnetic rod sleeve with adsorbed magnetic beads to the multiple rinsing liquid tanks of the rinsing area in sequence; Perform washing operation in each rinsing tank, including magnetic bead release, mixing, and re-adsorption; After the last rinsing, perform magnetic bead drying in the rinsing area.

5. The process method of nucleic acid extraction as claimed in claim 1 wherein, The elution collection step includes: Move the dried magnetic beads to the elution liquid tank of the collection area; Perform elution under heating conditions to make the nucleic acid dissolve in the elution liquid; After elution is completed, the magnetic rod adsorbs the magnetic beads and moves out of the collection area.

6. The process method of nucleic acid extraction according to any one of claims 1 to 5, wherein, The magnetic rod sleeve is disposable, and the adsorption and release of the magnetic beads are controlled by magnetic force when entering and leaving the liquid surface each time.

7. The process method of nucleic acid extraction according to any one of claims 1 to 5, wherein, The lysis area and the collection area are provided with heating modules for controlling the reaction temperature during operation.

8. The process method of nucleic acid extraction according to any one of claims 1 to 5, wherein, The rinsing area is provided with at least three rinsing tanks for multiple rinsing to improve the purity of the nucleic acid.

9. The process method of nucleic acid extraction according to any one of claims 1 to 5, wherein, The kit is a disposable consumable, and its position layout in the nucleic acid automatic extraction instrument is optimized and designed to ensure the stability of the kit and the convenience of operation during operation.

10. The process method of nucleic acid extraction according to any one of claims 1 to 5, wherein, The method is suitable for nucleic acid extraction in environmental samples, clinical samples or biological samples.

Images