Amino acid component detection device and detection method

Abstract

The invention discloses an amino acid component detection device and method, and relates to the technical field of amino acid detection.The amino acid component detection device comprises an amino acid analyzer body, a plurality of sets of insertion pipes are arranged on the amino acid analyzer body, every two insertion pipes form one set, each insertion pipe is communicated with a pipeline, and each pipeline is connected with the amino acid analyzer body; a plurality of reagent bottles with covers are arranged on the amino acid analyzer body; through operation of the auxiliary assembly, an adjustable limiting structure for the intubation tube is designed, and through three-point limiting of the intubation tube, accurate control and mechanical fixation of the perpendicularity and the insertion depth of the intubation tube are achieved, it is ensured that the intubation tube is in the optimal vertical state and the preset depth all the time, and it is ensured that the reagent drawing volume is accurate and consistent; the sampling error caused by inclination and displacement is avoided, the deviation of amino acid component detection data caused by sampling fluctuation is reduced, and the accuracy of the amino acid component detection data is ensured.

Description

Technical Field

[0001] This invention relates to the field of amino acid detection technology, specifically to an amino acid component detection device and detection method. Background Technology

[0002] In the food and pharmaceutical industries, amino acid detection is a key aspect of quality control. Amino acid analyzers, with their high sensitivity, high accuracy, and automated analytical capabilities, have become crucial equipment for achieving precise detection. Based on chromatographic separation principles, this instrument can perform qualitative and quantitative analysis of amino acids, primarily comprising modules for sample processing, injection, separation, detection, and reagent supply. Its workflow is as follows: after sample pretreatment, it enters the system, undergoes injection, chromatographic separation, derivatization reaction, and detection, ultimately outputting the analytical results. The reagent supply module is fundamental to ensuring the continuous and stable operation of the instrument. The pick-up tube and vent tube, as core components of this module, are responsible for accurately drawing reagents from the reagent bottle and balancing the pressure within the bottle, respectively. The reliable connection between these two components and the reagent bottle directly affects the stability of the analytical process.

[0003] Under the existing operating method, the operator needs to manually remove the reagent bottle cap, judge the insertion depth based on experience, and insert the aspiration tube and the venting tube into the reserved hole in the bottle cap respectively. Then, the external pipeline is connected to the instrument host to establish a complete reagent supply path.

[0004] However, this method has significant shortcomings: due to the lack of effective guiding and limiting structures, the cannula is unsupported after insertion, making it susceptible to tilting or displacement due to equipment vibration or tubing traction. This leads to changes in insertion depth, affecting sampling accuracy and causing fluctuations in test data, making it difficult to meet the stability requirements of amino acid detection. Furthermore, changing reagents requires repeated disassembly and reassembly of the cannula, which is cumbersome. In systems with multiple reagents supplied in parallel, exposed tubing is prone to tangling and becoming messy, increasing both operational difficulty and the risk of misoperation.

[0005] Therefore, an amino acid component detection device and detection method are proposed. Summary of the Invention

[0006] The purpose of this invention is to provide an amino acid component detection device and detection method to solve the problems mentioned in the background art.

[0007] To achieve the above objectives, the present invention provides the following technical solution: an amino acid component detection device and detection method, comprising an amino acid analyzer body, wherein the amino acid analyzer body is provided with multiple sets of insertion tubes, two insertion tubes per set, each insertion tube being connected to a conduit, each conduit being connected to the amino acid analyzer body, the amino acid analyzer body being provided with multiple capped reagent bottles, and the amino acid analyzer body being provided with multiple auxiliary components corresponding to the number of capped reagent bottles, the auxiliary components being divided into a limit adjustment component and a lifting and tidying component, both the limit adjustment component and the lifting and tidying component being located at the top of the amino acid analyzer body, the limit adjustment component being used to assist in providing vertical guidance for the insertion tubes and stabilizing the insertion state of the insertion tubes, the limit adjustment component including four pressure blocks and two protrusions, the pressure blocks having a semi-circular groove, the protrusions being configured as a raised shape with one side being flat, the lifting and tidying component being used to uniformly adjust the height position of the two insertion tubes and tidy the exposed parts of the two conduits, the lifting and tidying component including a support plate and two rubber rings, the support plate being used to simultaneously drive the two insertion tubes to move up and down synchronously, the rubber rings being used to prevent the conduits from moving due to external forces.

[0008] Furthermore, the limiting adjustment component includes a top cover, which is located on top of the amino acid analyzer body. A square groove is formed inside the top cover, and a bottom groove is formed at the bottom of the top cover. Four round holes are formed on the top cover, each containing a rubber ring. A rubber ring is fixedly connected to the top of the inner wall of the bottom groove. Two connecting rods are symmetrically inserted into the side wall of the top cover, each connecting rod penetrating the square groove. The four pressure blocks are divided into pairs, with the two pressure blocks in the same pair fixedly connected to the end of the connecting rod located inside the square groove. Two springs are symmetrically arranged, and the two springs are fixedly connected to the inner wall of the bottom groove and the adjacent pressure block. A spring is arranged in the center of the square groove, and the two ends of the spring are fixedly connected to the two adjacent pressure blocks. A connecting rod is fixedly connected to one end of each of the two connecting rods located outside the top cover. The bottom ends of the two connecting rods are inserted into the bottom groove, and a pressure block is fixedly connected to the bottom end of each of the two connecting rods. A rotating ring is rotatably connected to the side wall of the top cover, and two protrusions are symmetrically fixedly connected to the side wall of the rotating ring.

[0009] Furthermore, the lifting and sorting components also include a lifting groove, which is located on the top of the amino acid analyzer body. The support plate is fixedly connected to the bottom of the top cover. The top of the amino acid analyzer body has a lifting groove, and the support plate has an inner groove. The support plate has multiple slots arranged in a straight array, each slot penetrating the inner groove. Two rubber rings are symmetrically fixedly connected to the top of the support plate. A plug rod is placed on the top of the amino acid analyzer body.

[0010] Furthermore, the four round holes are arranged in pairs, with the two round holes in each pair corresponding vertically, meaning that the two round holes in each pair are on the same vertical axis. The two round holes in each pair are located at the top of the top cover and the top of the inner wall of the bottom groove, respectively. All four round holes are connected to the square groove, and the two round holes in the same pair are used to insert the same insertion tube.

[0011] Furthermore, both pressure block one and pressure block two are made of rubber, and pressure block one and pressure block two are provided with anti-slip texture. The four pressure blocks one are arranged in groups of two adjacent ones. The two pressure blocks one in the same group belong to two different connecting rods one. The two pressure blocks one in the same group are symmetrically distributed along the vertical axis of the adjacent circular holes.

[0012] Furthermore, both connecting rods are located on the rotation path of the two protrusions, which are configured to protrude outward from the rotating ring, and the side of the protrusion away from the rotating ring is configured as a plane.

[0013] Furthermore, the bottom end of the support plate is inserted into the lifting groove, the insertion rod is inserted into the slot, and the pipeline is inserted into the rubber ring three.

[0014] A method for detecting amino acid composition includes the following steps: Step 1: Rotate the ring on the top cover. The protrusion pushes the connecting rod 2 outward from the connecting rod 1, causing the pressure block 1 and pressure block 2 to separate synchronously. This causes the spring 1 and spring 2 to undergo elastic deformation, maintaining the structural separation state to unlock the tube installation channel.

[0015] Step 2: Insert the two cannulas into the corresponding round holes from the top of the top cover, pass through the rubber ring and extend out of the bottom of the top cover. Use the elastic squeezing force of the rubber ring to initially fix the cannulas and ensure that the length of their bottom extension is stable.

[0016] Further, in step three: place the capped reagent bottle in the corresponding position marked on the main body of the amino acid analyzer, pull out the insertion rod and push the top cover downwards to make the support plate slide down along the lifting groove, so that the insertion tube is inserted into the capped reagent bottle through the pre-set hole in the bottle cap. After the rubber ring two touches the bottle cap and deforms, insert the insertion rod into the corresponding slot to fix the height of the top cover.

[0017] Further, in step four: adjust the insertion depth of the cannula according to the volume and shape of the reagent bottle with the cap (the suction tube should be below the liquid surface without touching the bottom, and the venting tube should be above the liquid surface). Then, rotate the rotating ring in the opposite direction to disengage the protrusion from the connecting rod two. Under the reset action of spring one and spring two, the pressure block and the cannula form a three-point locking limit. The pressure block two abuts against the bottle cap to fix the top cap. At the same time, the exposed part of the tubing is tidied up and fixed by the rubber ring three.

[0018] Step 5: Rotate the ring to unlock the structure, pull the support plate upward to simultaneously pull out the capped reagent bottle with the insertion tube, insert the insertion rod into the bottom slot to fix the top cap height, and repeat steps 3 and 4 after replacing the new capped reagent bottle to restore the detection state. For reagent bottles of the same specifications, there is no need to readjust the insertion depth.

[0019] Compared with the prior art, the beneficial effects of the present invention are: Existing technology relies on human experience to determine the insertion depth of the cannula, and the cannula is in an unrestrained state after insertion.

[0020] By utilizing the auxiliary components, an adjustable limiting structure for the insertion tube was designed. Through three-point limiting of the insertion tube, precise control and mechanical fixation of the verticality and insertion depth of the insertion tube were achieved, ensuring that the insertion tube is always in the optimal vertical state and preset depth. This ensures the accurate and consistent volume of reagents drawn, avoids sampling errors caused by tilting or displacement, reduces deviations in amino acid component detection data caused by sampling fluctuations, and ensures the accuracy of amino acid component detection data.

[0021] The existing method requires manual insertion and removal of the tubing connection multiple times when changing reagents, which is cumbersome.

[0022] By utilizing the auxiliary components, a system was designed to enable rapid connection and separation of reagent bottles and tubes while preserving the preset insertion depth of the tubes. This eliminates the need for manual handling of each tube individually, simplifies the reagent replacement process, and improves the efficiency of amino acid component detection.

[0023] By using auxiliary components, exposed tubing is neatly organized, avoiding tangled and messy tubing that could lead to complicated operations and misoperations. This improves the convenience and reliability of the amino acid analyzer in detecting amino acid components. Attached Figure Description

[0024] Figure 1 This is a three-dimensional schematic diagram of the overall device of the present invention; Figure 2 This is a schematic diagram showing the positions of the capped reagent bottle, cannula, tubing, and other structures of the present invention. Figure 3 This is a detailed schematic diagram of the structure of the cannula, tubing, and capped reagent bottle of the present invention. Figure 4 This is a cross-sectional schematic diagram of the structure of the amino acid analyzer body, the capped reagent bottle, the support plate, etc. of the present invention. Figure 5 This is a cross-sectional schematic diagram of the top cover, square groove, bottom groove, and other structures of the present invention; Figure 6 For the present invention Figure 5 Enlarged view of point A in the middle; Figure 7This is a partial cross-sectional schematic diagram of the top cover, pressure block, and other structures of the present invention; Figure 8 For the present invention Figure 7 Enlarged view of point B in the middle; Figure 9 This is an exploded view of the top cover, rubber ring II, swivel ring, and other structures of the present invention; Figure 10 This is a detailed schematic diagram of the structure of the present invention, including the pressure block 1, spring 2, and connecting rod 1.

[0025] In the picture: 11. Amino acid analyzer body; 12. Intubation tube; 13. Tubing; 14. Reagent bottle with cap; The auxiliary components include the following: Limit adjustment components: 21. Top cover; 22. Square groove; 23. Bottom groove; 24. Round hole; 25. Rubber ring one; 26. Rubber ring two; 27. Connecting rod one; 28. Pressure block one; 29. ​​Spring one; 210. Spring two; 211. Connecting rod two; 212. Pressure block two; 213. Rotary ring; 214. Protrusion; Lifting and straightening components: 215, support plate; 216, lifting groove; 217, inner groove; 218, slot; 219, rubber ring three; 220, insertion rod. Detailed Implementation

[0026] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present invention.

[0027] The embodiments provided by this invention: Example 1: Please see Figures 1 to 10 As shown, an amino acid component detection device and detection method include an amino acid analyzer body 11. The amino acid analyzer body 11 is provided with multiple sets of tubes 12, with two tubes 12 forming a group. Each tube 12 is connected to a conduit 13, and each conduit 13 is connected to the amino acid analyzer body 11. The amino acid analyzer body 11 is provided with multiple capped reagent bottles 14, and each capped reagent bottle 14 corresponds to a set of tubes 12 and conduits 13.

[0028] Among them, the amino acid analyzer body 11, the insertion tube 12, the tubing 13, and the capped reagent bottle 14 are all existing known technologies. The amino acid analyzer body 11 is based on the principle of chromatographic separation and can perform qualitative and quantitative analysis of amino acids. After sample injection, chromatographic separation, derivatization reaction and detection, the amino acid analyzer body 11 finally outputs the analysis results of the amino acid component detection.

[0029] In this set, the two insertion tubes 12 are the existing draw tube and vent tube, respectively. In the prior art, the cap of the reagent bottle 14 has two pre-set holes corresponding to the insertion tubes 12, facilitating direct insertion of the insertion tubes 12 into the bottle. It should be noted that in the prior art, when the two insertion tubes 12 are inserted into the capped reagent bottle 14, the draw tube needs to be inserted to a depth below the liquid surface but not to the bottom, while the vent tube needs to be inserted to a position above the liquid surface. However, the insertion positions of the draw tube and vent tube will vary depending on factors such as the volume and shape of the capped reagent bottle 14. That is, the insertion depth of the draw tube and vent tube needs to be adjusted according to the actual capped reagent bottle 14 used.

[0030] In the prior art, the amino acid analyzer body 11 is provided with a mark (not shown) for indicating the placement position of the capped reagent bottle 14.

[0031] The amino acid analyzer body 11 is equipped with multiple auxiliary components corresponding to the number of capped reagent bottles 14. The auxiliary components are divided into limit adjustment components and lifting and tidying components, both of which are located on the top of the amino acid analyzer body 11.

[0032] The limit adjustment component is used to assist in providing vertical guidance for the cannula 12 and to stabilize the insertion state of the cannula 12.

[0033] The limit adjustment component includes a top cover 21, which is located on top of the amino acid analyzer body 11. A square groove 22 is formed inside the top cover 21, and a bottom groove 23 is formed at the bottom of the top cover 21. Four round holes 24 are formed on the top cover 21, and a rubber ring 25 is fixedly connected to each hole 24. A rubber ring 26 is fixedly connected to the top of the inner wall of the bottom groove 23. Two connecting rods 27 are symmetrically inserted into the side wall of the top cover 21, each connecting rod 27 penetrating into the square groove 22. Two pressure blocks 28 are fixedly connected to the ends of the two connecting rods 27 inside the square groove 22. Two springs 28 are symmetrically arranged inside the square groove 22. 9. Two springs 29 are fixedly connected to the inner wall of the bottom groove 23 and the adjacent pressure block 28 respectively. A spring 210 is set in the center of the square groove 22. The two ends of the spring 210 are fixedly connected to the side of the two adjacent pressure blocks 28 that are close to each other. One end of each of the two connecting rods 27 located outside the top cover 21 is fixedly connected to a connecting rod 211. The bottom ends of the two connecting rods 211 are inserted into the bottom groove 23. The bottom ends of the two connecting rods 211 are fixedly connected to a pressure block 212. A rotating ring 213 is rotatably connected to the side wall of the top cover 21. Two protrusions 214 are symmetrically fixedly connected to the side wall of the rotating ring 213.

[0034] Where: Reference Figure 6 and Figure 9 As shown, the four circular holes 24 are arranged in pairs, with the two holes 24 in each pair corresponding vertically, meaning they are on the same vertical axis. The two holes 24 in each pair are located at the top of the top cover 21 and the top of the inner wall of the bottom groove 23, respectively. All four holes 24 are connected to the square groove 22. The two holes 24 in the same pair are used to insert the same insertion tube 12. The function of the rubber ring 25 is to press and compress the insertion tube 12, and through the deformation of the rubber material, it can adapt to insertion tubes 12 of different diameters.

[0035] Where: Reference Figure 8 and Figure 10 As shown, both pressure block 1 28 and pressure block 2 212 are made of rubber, and both have anti-slip textures. The four pressure blocks 1 28 are grouped in pairs, but the two pressure blocks 1 28 in the same group belong to two different connecting rods 1 27. A semi-circular groove is provided on the side of each pair of pressure blocks 1 28 in the same group, and the two pressure blocks 1 28 in the same group are symmetrically distributed along the vertical axis of adjacent circular holes 24. The function of pressure block 1 28 is to abut against the limiting insertion tube 12. The function of pressure block 2 212 is to abut against the cap of the capped reagent bottle 14.

[0036] Specifically, both connecting rods 211 are located on the rotation path of the two protrusions 214. The protrusions 214 are designed to protrude outwards from the rotating ring 213, and the side of the protrusion 214 away from the rotating ring 213 is designed as a plane. The function of the protrusions 214 is to push the two connecting rods 27 outwards from the two connecting rods 211, and the planar design ensures that when the plane of the protrusion 214 contacts the connecting rod 211, the separation distance between the two connecting rods 27 is maximized.

[0037] The lifting and straightening component is used to uniformly adjust the height of the two insertion tubes 12 and straighten the exposed parts of the two pipes 13.

[0038] The lifting and adjusting components include a support plate 215, which is fixedly connected to the bottom of the top cover 21. The top of the amino acid analyzer body 11 has a lifting groove 216, and the support plate 215 has an inner groove 217. The support plate 215 has multiple slots 218 arranged in a linear array, and each slot 218 passes through the inner groove 217. The top of the support plate 215 is symmetrically fixedly connected to two rubber rings 219, and the top of the amino acid analyzer body 11 has a plug rod 220.

[0039] Where: Reference Figure 2 and Figure 4 As shown, the bottom end of the support plate 215 is inserted into the lifting groove 216. The function of the lifting groove 216 is to provide vertical guidance for the support plate 215. The function of the support plate 215 is to simultaneously drive the two insertion tubes 12 to move up and down synchronously.

[0040] The inner groove 217 serves to accommodate the portion of the pipe 13 that is not exposed above the top of the support plate 215.

[0041] Among them, the insertion rod 220 is inserted into the slot 218.

[0042] Wherein: Pipe 13 is inserted into rubber ring 219. The function of rubber ring 219 is to resist and compress pipe 13 through the deformation capacity and friction of rubber material, thereby preventing pipe 13 from moving due to external force.

[0043] The function of slot 218 is as follows: Through the array of multiple slots 218, the user can select to insert the rod 220 into a corresponding slot 218, and make the bottom of the rod 220 fit against the top surface of the amino acid analyzer body 11, thereby fixing the height of the support plate 215 inside the lifting groove 216.

[0044] In the initial state of the auxiliary components, i.e., when the amino acid analyzer body 11 is not performing amino acid component detection, the capped reagent bottle 14 is not placed on top of the amino acid analyzer body 11. The two insertion tubes 12 are not yet inserted into the capped reagent bottle 14 through its cap. The two protrusions 214 on the top cover 21 are not in contact with the connecting rod 211. Under the elastic force of the spring 210 and the two springs 29, the four pressure blocks 28 are in contact with each other, and the insertion rod 220 is inserted into the bottommost slot 218. The bottom surface of the insertion rod 220 is in contact with the top surface of the amino acid analyzer body 11, meaning that the support plate 215 is at its maximum height extending from the lifting groove 216. The two insertion tubes 12 are not inserted into their corresponding rubber rings 25.

[0045] When the auxiliary components are running, i.e., when the amino acid analyzer body 11 needs to perform amino acid component detection, the user needs to connect the two tubing 12 to the capped reagent bottle 14 so that the amino acid analyzer body 11 can receive reagent supply from the capped reagent bottle 14 through the tubing 12 and tubing 13. The specific operation is as follows: The user first rotates the rotating ring 213. As the rotating ring 213 rotates on the top cover 21, the two protrusions 214 rotate synchronously with it. With the rotation of the protrusions 214, they gradually contact the two connecting rods 211. Since the protrusions 214 protrude outwards on the rotating ring 213, as they contact the corresponding connecting rods 211, the two connecting rods 211 are pushed away from the top cover 21, meaning they separate from each other. During this separation, when the two connecting rods 211 contact the flat portion of their respective protrusions 214, the separation distance between them is at its maximum, which is also the maximum separation distance between the two connecting rods 211.

[0046] During the separation of the two connecting rods 27 and 211, each connecting rod 27 drives its respective two pressure blocks 28 to move synchronously. This allows the two pressure blocks 28, which were initially in contact with each other, to separate and move away from each other. Simultaneously, the two pressure blocks 212 are also moved away from each other by the separation of the two connecting rods 211. As all four pressure blocks 28 move, spring 210 and spring 29 undergo elastic deformation, tending to pull the corresponding pressure blocks 28 back to their contact state. However, because the planar portion of the protrusion 214 contacts the connecting rod 211, the two connecting rods 211 and 27 separate to their maximum distance. This fixes the positional state of the separated structures (connecting rods 211, 27, pressure blocks 28, and pressure blocks 212) and the elastic deformation state of spring 210 and spring 29.

[0047] After completion, the user inserts the two cannulas 12 into the two round holes 24 located at the top of the top cover 21 from the top direction, and pushes the cannulas 12 downwards until they pass through the two round holes 24 located at the bottom. During this process, the cannulas 12 pass through the inner ring surface of the rubber ring 25 and compress the rubber ring 25 to produce elastic deformation. At this time, the bottom end of the cannulas 12 is located at the bottom of the top cover 21. The compressive force and friction provided by the elastic deformation of the rubber material of the rubber ring 25 can initially fix the cannulas 12. That is, without the influence of external force, the rubber ring 25 can fix the length of the bottom end of the cannulas 12 extending out of the bottom surface of the top cover 21. Subsequently, the user places the capped reagent bottle 14 at the corresponding position on the amino acid analyzer body 11 and pulls out the insertion rod 220 from the slot 218, so that the support plate 215 is no longer limited. Then, the user pushes the top cover 21 downward to move it, so that the support plate 215 slides downward in the lifting groove 216. During this process, the top cover 21 drives the two insertion tubes 12, which have been initially fixed, to move downward synchronously, so that the two insertion tubes 12 are inserted into the capped reagent bottle 14 through the reserved hole on the cap of the capped reagent bottle 14. At the same time, the rubber ring 26 contacts the top of the cap of the reagent bottle 14 and undergoes elastic deformation. When the top cover 21 can no longer move downward, the user inserts the rod 220 into the slot 218 closest to the top of the amino acid analyzer body 11, and completely extends the other end of the rod 220 through the support plate 215, so that the bottom of the rod 220 is in contact with the top surface of the amino acid analyzer body 11. Then, the support plate 215 can no longer move downward from the lifting groove 216, thus realizing the height adjustment of the top cover 21.

[0048] At this point, the user can adjust the length of the two insertion tubes 12 extending from the bottom according to the size of the reagent bottle 14 with the cap. That is, the user can push the insertion tubes 12 up and down within the top cover 21. Although the single insertion tube 12 is pressed against the two rubber rings 25, the pushing force provided by the rubber rings 25 is not enough to completely limit the insertion tube 12. The user can still push the insertion tube 12 up and down within the top cover 21. That is, the user can freely adjust the position of the bottom of the insertion tube 12 within the reagent bottle 14 with the cap. When the user inserts the suction tube of the two insertion tubes 12 into the reagent bottle 14 below the liquid surface but not touching the bottom, and inserts the vent tube into the reagent bottle 14 above the liquid surface, the user no longer needs to adjust the position of the insertion tubes 12.

[0049] After adjustment, the user continues to rotate the ring 213, causing the two protrusions 214 to rotate until they no longer contact the two connecting rods 211. Then, under the elastic restoring action of the spring 210 and the two springs 29, the previously separated structures are brought into a contact limit state. Then, the two connecting rods 27 and the two connecting rods 211 move towards each other, causing the two pressure blocks 28 of the same group to move towards each other. Then, the arc groove side of the two pressure blocks 28 of the same group gradually contacts the insertion tube 12. With the continuous gathering action provided by the elastic force of the springs 29 and 210, both insertion tubes 12 are pressed by the two sets of pressure blocks 28. At this time, under the anti-slip texture of the pressure block 28 and the pushing and locking action provided by the springs 29 and 210, the two insertion tubes 12 are locked and limited, so that they cannot be moved further.

[0050] Simultaneously, the two connecting rods 211 move towards each other, causing the two pressure blocks 212 to move towards each other synchronously. During this process, the inner surfaces of the two pressure blocks 212 contact the cap of the reagent bottle 14. As the two pressure blocks 212 are squeezed, they undergo elastic deformation. At this time, under the action of the contact and squeezing between the pressure blocks 212 and the cap of the reagent bottle 14, as well as the friction provided by the rubber material of the pressure blocks 212, the position of the top cover 21 can be fixed.

[0051] It should be noted that by tightening and fixing the top cap 21 to the capped reagent bottle 14, the top cap 21 and the capped reagent bottle 14 are connected as a whole, which can ensure the stability of the position of the two tubes 12 inserted into the capped reagent bottle 14.

[0052] It should be noted that as the two sets of pressure blocks 28 lock and limit the two insertion tubes 12 respectively, the insertion tubes 12 are in a three-point limiting position formed by being pressed and squeezed by the upper and lower rubber rings 25 and locked and limited by the two pressure blocks 28 in the middle. This prevents the insertion tubes 12 from tilting and ensures that the insertion tubes 12 are inserted vertically into the capped reagent bottle 14.

[0053] It should be noted that in the above process, the rubber ring 26 acts as a sealing gasket, which can prevent the reagent in the capped reagent bottle 14 from evaporating outward from the insertion part of the tube 12 and the capped reagent bottle 14.

[0054] It should be noted that if the user needs to adjust the insertion position of the cannula 12 when the reagent bottle 14 with the cap is inserted into the cannula 12, the user only needs to rotate the rotating ring 213 to make the protrusion 214 push the separation connecting rod 27 and connecting rod 211 again to achieve the contact locking state of the cannula 12. At this time, the cannula 12 is only abutted by the upper and lower rubber rings 25. The user can adjust the bottom position of the cannula 12 according to the desired state. After the adjustment is completed, rotate the rotating ring 213 again to reset the two pressure blocks 28 and re-lock the cannula 12.

[0055] It should be noted that during the above process, the user can adjust the portion of the tube 13 exposed at the top of the rubber ring 219 so that the part of the tube 13 exposed at the top of the rubber ring 219 and connected to the insertion tube 12 is taut but does not affect the internal channel. The adjustment method is to directly pull or insert the tube 13 into the inner groove 217. After adjustment, the rubber ring 219, through the deformation ability and friction of the rubber material, can resist and limit the tube 13, thereby preventing the tube 13 from moving due to external forces when no adjustment is needed, and ensuring the neat state of the exposed portion of the tube 13.

[0056] Since the reagent in the capped reagent bottle 14 is a consumable, when the reagent in the capped reagent bottle 14 is exhausted and needs to be replaced, the user rotates the rotating ring 213 to separate the protrusion 214 from the connecting rod 1 27 and the connecting rod 211, etc. At this time, the pressure block 212 no longer abuts against the cap of the capped reagent bottle 14, and the top cover 21 and the capped reagent bottle 14 are in contact. The user directly pulls the support plate 215 upward, so that the support plate 215 moves upward within the support plate 215. As the support plate 215 moves upward, the support plate 215 drives the two insertion tubes 12 squeezed by the rubber ring 1 25 to move upward synchronously through the top cover 21, so that the insertion tubes 12 are pulled out of the capped reagent bottle 14. Then, the insertion rod 220 is aligned with the bottom slot 218 and inserted, thereby adjusting the position of the top cover 21 to the highest and limiting position. After completion, the user replaces the capped reagent bottle 14 and places it in the corresponding position on the amino acid analyzer body 11. The user then pulls out the insertion rod 220 and pushes the top cover 21 down, causing the two insertion tubes 12 to move down and be inserted into the newly replaced capped reagent bottle 14 through the holes in the cap. If the volume and specifications of the replaced capped reagent bottle 14 are the same as the previously used one, the user does not need to adjust the positions of the two insertion tubes 12 and can continue using their current positions. Only the rotating ring 213 needs to be rotated to reset the pressure blocks 28 and 212, thus locking the insertion tubes 12 and the top cover 21 to the capped reagent bottle 14. Then, the insertion rod 220 is inserted into the corresponding slot 218. If the volume and specifications are different, the user can directly adjust the height of the insertion tubes 12 without rotating the rotating ring 213.

[0057] It should be noted that during the process of replacing the new capped reagent bottle 14, the two tubes 12 are disassembled and reassembled synchronously. Since the exposed part of the tubing 13 has been pre-tightened, the overall process is simple and fast and can avoid misoperation.

[0058] In summary, the following beneficial effects can be achieved by running the auxiliary components: The existing technology relies on human experience to determine the insertion depth of the cannula 12, and the cannula 12 is in an unrestrained state after insertion.

[0059] By utilizing the auxiliary components, an adjustable limiting structure for the insertion tube 12 was designed. Through the three-point limiting of the insertion tube 12, precise control and mechanical fixation of the verticality and insertion depth of the insertion tube 12 were achieved, ensuring that the insertion tube 12 is always in the optimal vertical state and preset depth. This ensures the accurate and consistent volume of reagents drawn, avoids sampling errors caused by tilting or displacement, reduces deviations in amino acid component detection data caused by sampling fluctuations, and ensures the accuracy of amino acid component detection data.

[0060] The existing method requires manual plugging and unplugging of the tube 12 multiple times when changing reagents, which is cumbersome.

[0061] By utilizing the auxiliary components, a system was designed to enable rapid connection and separation of the reagent bottle and the tube 12 while maintaining the preset insertion depth of the tube 12. This eliminates the need for manual handling of each tube 12, simplifies the reagent replacement process, and improves the efficiency of amino acid component detection.

[0062] By using auxiliary components, the exposed pipes 13 are gathered together, avoiding the cumbersome operation and misoperation caused by the tangled pipes 13, thereby improving the convenience and reliability of the amino acid analyzer body 11 in detecting amino acid components.

[0063] Example 2: A method for detecting amino acid composition includes the following steps: Step 1: Rotate the rotating ring 213 on the top cover 21. The protrusion 214 pushes the connecting rod 211 and the connecting rod 27 outward, causing the pressure block 28 and the pressure block 212 to separate synchronously. This causes the spring 29 and the spring 210 to undergo elastic deformation, maintaining the separation state of the structure to unlock the installation channel of the insertion tube 12.

[0064] Step 2: Insert the two insertion tubes 12 into the corresponding round holes 24 from the top of the top cover 21, pass through the rubber ring 25 and extend out of the bottom of the top cover 21. Use the elastic squeezing force of the rubber ring 25 to initially fix the insertion tubes 12 and ensure that the length of their bottom extension is stable.

[0065] Step 3: Place the capped reagent bottle 14 in the corresponding position marked on the main body 11 of the amino acid analyzer. After pulling out the insertion rod 220, push the top cover 21 downward so that the support plate 215 slides down along the lifting groove 216, driving the insertion tube 12 to be inserted into the capped reagent bottle 14 through the pre-set hole in the bottle cap. After the rubber ring 26 touches the bottle cap and deforms, insert the insertion rod 220 into the corresponding slot 218 to fix the height of the top cover 21.

[0066] Step 4: Adjust the insertion depth of the cannula 12 according to the volume and shape of the reagent bottle 14 with the cap. The suction tube should be below the liquid surface without touching the bottom, and the vent tube should be above the liquid surface. Then, rotate the rotating ring 213 in the opposite direction to disengage the protrusion 214 from the connecting rod 211. Under the reset action of the spring 1 29 and the spring 2 210, the pressure block 1 28 forms a three-point locking limit on the cannula 12. The pressure block 2 212 abuts against the bottle cap to fix the top cover 21. At the same time, tidy up the exposed part of the pipeline 13 and fix it by limiting it with the rubber ring 3 219.

[0067] Step 5: Rotate the ring 213 to unlock the structure, pull the support plate 215 upward to drive the insertion tube 12 to simultaneously pull out the capped reagent bottle 14, insert the insertion rod 220 into the bottom slot 218 to fix the height of the top cover 21, and after replacing the new capped reagent bottle 14, repeat steps 3 and 4 to restore the detection state. For reagent bottles of the same specifications, there is no need to readjust the depth of the insertion tube 12.

[0068] It should be noted that the terms “comprising,” “including,” or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0069] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An amino acid component detection device, comprising an amino acid analyzer body (11), a plurality of sets of insertion tubes (12) are arranged on the amino acid analyzer body (11), two insertion tubes (12) form a set, each insertion tube (12) is respectively connected with a pipeline (13), each pipeline (13) is connected with the amino acid analyzer body (11), a plurality of reagent bottles (14) with covers are arranged on the amino acid analyzer body (11), characterized in that: The amino acid analyzer body (11) is provided with a plurality of auxiliary assemblies corresponding to the number of reagent bottles (14) with lids, the auxiliary assemblies are divided into limiting adjustment components and lifting arrangement components, the limiting adjustment components and the lifting arrangement components are located at the top of the amino acid analyzer body (11), the limiting adjustment components are used for assisting in providing vertical guidance for the cannulas (12) and stabilizing the insertion state of the cannulas (12), the limiting adjustment components include four pressing blocks one (28) and two protrusions (214), the pressing blocks one (28) are provided with semicircular grooves, the protrusions (214) are provided in the form of protrusions with one side being flat, the lifting arrangement components are used for uniformly adjusting the height positions of the two cannulas (12) and arranging the exposed parts of the two pipelines (13), the lifting arrangement components include a support plate (215) and two rubber rings three (219), the support plate (215) is used for simultaneously driving the two cannulas (12) to move up and down synchronously, and the rubber rings three (219) are used for preventing the pipelines (13) from moving due to external force. ​ 2. The amino acid composition detection device according to claim 1, characterized in that: The limiting adjustment components include a top cover (21), the top cover (21) is arranged at the top of the amino acid analyzer body (11), the top cover (21) is provided with a square groove (22) in the inside, the bottom of the top cover (21) is provided with a bottom groove (23), the top cover (21) is provided with four circular holes (24), one rubber ring one (25) is fixedly connected in each circular hole (24), a rubber ring two (26) is fixedly connected to the top of the inner wall of the bottom groove (23), two connecting rods one (27) are inserted into the sidewall of the top cover (21) in a staggered and symmetrical manner, the two connecting rods one (27) penetrate into the inside of the square groove (22), the four pressing blocks one (28) are divided into two groups, the two pressing blocks one (28) in the same group are fixedly connected to one end of the connecting rod one (27) located in the inside of the square groove (22), two springs one (29) are symmetrically arranged in the inside of the square groove (22), the two springs one (29) are fixedly connected with the inner wall of the bottom groove (23) and the adjacent pressing blocks one (28) respectively, a spring two (210) is arranged at the central position in the square groove (22), the two ends of the spring two (210) are fixedly connected with the sides of the adjacent two pressing blocks one (28) close to each other, one connecting rod two (211) is fixedly connected to one end of each of the two connecting rods one (27) located outside the top cover (21), the bottom ends of the two connecting rods two (211) penetrate into the bottom groove (23), one pressing block two (212) is fixedly connected to the bottom end of each of the two connecting rods two (211), a rotating ring (213) is rotatably connected to the sidewall of the top cover (21), and the two protrusions (214) are fixedly connected to the sidewall of the rotating ring (213) in a symmetrical manner.

3. The amino acid composition detection device of claim 1, wherein: The lifting arrangement component further comprises a lifting groove (216) formed in the top of the amino acid analyzer body (11), the support plate (215) is fixedly connected to the bottom of the top cover (21), the top of the amino acid analyzer body (11) is provided with the lifting groove (216), the support plate (215) is provided with an inner groove (217), a plurality of insertion grooves (218) are linearly arranged on the support plate (215), each insertion groove (218) penetrates the inner groove (217), two rubber ring threes (219) are symmetrically fixed to the top of the support plate (215), and the insertion rod (220) is placed on the top of the amino acid analyzer body (11).

4. The amino acid composition detection device of claim 1, wherein: The four round holes (24) are grouped into two groups, the two round holes (24) in one group are arranged in a corresponding upper and lower position, that is, the two round holes (24) in one group are on the same vertical axis, and the two round holes (24) in one group are located at the top of the top cover (21) and the inner wall top of the bottom groove (23) respectively, the four round holes (24) are all communicated with the square groove (22), and the two round holes (24) in the same group are used for inserting a same insertion tube (12).

5. The amino acid composition detection device of claim 1, wherein: The pressing block one (28) and the pressing block two (212) are both made of rubber material, and the pressing block one (28) and the pressing block two (212) are provided with anti-skid textures, the four pressing block ones (28) are grouped into two adjacent groups, the two pressing block ones (28) in the same group respectively belong to two different connecting rods one (27), and the two pressing block ones (28) in the same group are symmetrically distributed along the vertical axis of the adjacent round hole (24).

6. The amino acid composition detection device of claim 1, wherein: The two connecting rods two (211) are located on the rotation path of the two protrusions (214), the protrusion (214) is provided in a shape protruding outward from the rotating ring (213), and the side of the protrusion (214) away from the rotating ring (213) is provided in a plane.

7. The amino acid composition detection device of claim 2, wherein: The bottom end of the support plate (215) is inserted into the lifting groove (216), the insertion rod (220) is inserted into the insertion groove (218), and the pipeline (13) is inserted into the rubber ring three (219).

8. A method of detecting an amino acid composition, characterized by: An amino acid component detection device as claimed in claims 1-7, Step one: rotate the rotating ring (213) on the top cover (21), push the connecting rod two (211) and the connecting rod one (27) outward by the protrusion (214), drive the pressing block one (28) and the pressing block two (212) to separate synchronously, make the spring one (29) and the spring two (210) elastically deform, and keep the structure in a separated state to unlock the insertion tube (12) installation channel; Step two: insert the two insertion tubes (12) into the corresponding round holes (24) from the top of the top cover (21), pass through the rubber ring one (25) and extend out of the bottom of the top cover (21), and utilize the elastic extrusion force of the rubber ring one (25) to realize preliminary fixation of the insertion tube (12) and ensure that the bottom end has a stable extension length.

9. The method of claim 8, wherein: Step three: Put the reagent bottle (14) with cover in the corresponding position of the amino acid analyzer body (11), pull out the insertion rod (220) and push down the top cover (21), so that the support plate (215) slides down along the lifting groove (216), driving the insertion tube (12) to insert into the reagent bottle (14) through the pre-set hole of the bottle cover. After the second rubber ring (26) contacts the bottle cover and deforms, insert the insertion rod (220) into the corresponding slot (218) to fix the height of the top cover (21).

10. The method of claim 8, wherein: Step four: Adjust the insertion depth of the insertion tube (12) according to the volume and type of the reagent bottle (14) (the burette does not touch the bottom below the liquid surface, and the exhaust pipe is above the liquid surface), then rotate the rotating ring (213) in the opposite direction to make the protrusion (214) disengage from the second connecting rod (211). Under the reset action of spring one (29) and spring two (210), the first pressing block (28) forms a three-point locking limit for the insertion tube (12), and the second pressing block (212) contacts the bottle cover to fix the height of the top cover (21). At the same time, arrange the exposed part of the pipeline (13) and fix it by the third rubber ring (219); Step five: Rotate the rotating ring (213) to unlock the structure, pull up the support plate (215) to drive the insertion tube (12) to withdraw from the reagent bottle (14) with cover, insert the insertion rod (220) into the bottommost slot (218) to fix the height of the top cover (21), and replace the new reagent bottle (14) with cover. Repeat steps three and four to restore the detection state, and no need to adjust the depth of the insertion tube (12) for the same specification reagent bottle.

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