A fluorescent protein selection marker, staygold-arnF r Genes and their applications
By replacing the L-leucine codon with the rare codon TTA in the Staygold and arnF genes and linking it with a flexible protein peptide, a fluorescent protein screening marker, staygold-arnFr, was constructed. This solved the problem of low screening efficiency in the L-leucine production process in existing technologies and enabled efficient and low-cost strain screening.
Patent Information
- Application Number
- CN202610225874.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2026-02-26
- Publication Date
- 2026-05-26
- Estimated Expiration
- 2046-02-26
AI Technical Summary
In the existing technology, the production process of L-leucine lacks efficient and specific screening markers, resulting in low screening efficiency, high cost, and difficulty in obtaining high-yield mutant strains with industrial application potential.
A fluorescent protein screening marker, the staygold-arnFr gene, was designed. By replacing the L-leucine codon with the rare codon TTA in the Staygold and arnF genes and linking it with a flexible protein peptide, a recombinant vector and recombinant bacteria were constructed, enabling efficient screening of L-leucine-producing strains.
It improved the screening efficiency and accuracy of high-yield L-leucine strains, reduced screening costs, and enhanced the sensitivity and specificity of screening.