Batch separation device for pine wood nematode wood samples

Abstract

The invention discloses a pine wood nematode wood sample batch separation device, and relates to the technical field of plant quarantine, the pine wood nematode wood sample batch separation device comprises a first base and a partition plate; the second base is fixedly installed on the rear side face of the first base, the height of the first base is 0.25 m, the height of the second base is 0.6 m, the first base and the second base are combined in a stepped mode, the partition plates are evenly fixed in the first base and the second base, round holes are evenly formed in the top face of the first base and the top face of the second base, and the partition plates are arranged in the round holes. According to the device for separating the wood samples of the pine wood nematodes in batches, the plurality of glass funnels can be placed in the plurality of round holes, so that the samples of different batches can be treated at the same time, the operation is simple, the operation is convenient, and the efficiency is high. By rotating the screw rod, the pine wood nematode wood sample can be tightly pressed in the glass funnel by the pressing plate, so that the pine wood nematode wood sample is prevented from floating upwards to influence the experiment effect.

Description

Technical Field

[0001] This invention relates to the field of plant quarantine technology, specifically to a device for the batch separation of pine wood nematode wood samples. Background Technology

[0002] Pine wilt nematode is transmitted to pine trees by vector insects such as the pine sawyer beetle. It parasitizes the pine tree, causing rapid death of the entire tree. Listed as a key managed invasive alien species, it is a Class II quarantine pest in my country. To examine pine trees infected with or suspected of having pine wilt nematode, it is generally necessary to isolate the nematodes from the wood sample. The current technique, the Bellman funnel method, typically involves placing a funnel on an iron stand, connecting a flexible tube to the funnel, and installing a stop valve on the tube. The diseased wood sample is then immersed in water while waiting for the nematodes to separate. The main problems with this technique are: the depth of commonly used laboratory funnels is insufficient, failing to completely submerge the wood sample, leading to experimental errors; the iron stand is easily knocked over in the laboratory; and it is inefficient, unable to process multiple samples simultaneously. The shallow dish funnel method, which uses small sieves to separate nematodes, often suffers from experimental errors due to wear and tear after repeated use. Therefore, it is necessary to develop a device that can separate pine wood nematodes in batches to improve separation efficiency while ensuring its stability and laboratory safety. To this end, we propose a device for the batch separation of pine wood nematode wood samples. Summary of the Invention

[0003] The technical problem to be solved by the present invention is to overcome the existing defects and provide a device for batch separation of pine wood nematode wood samples. The device has multiple round holes to place multiple glass funnels so as to process different batches of samples at the same time. Rotating the screw can press the pine wood nematode wood sample tightly inside the glass funnel by the pressure plate to prevent the pine wood nematode wood sample from floating and affecting the experimental results. This device can effectively solve the problems in the background technology.

[0004] To achieve the above objectives, the present invention provides the following technical solution: a device for batch separation of pine wood nematode samples, comprising a first base and a partition; First base: A second base is fixedly installed on the rear side. The first base has a layer height of 0.25m, and the second base has a layer height of 0.6m. The first base and the second base are combined in a stepped manner. The partition is evenly fixed inside the first base and the second base. The top surfaces of the first base and the second base are evenly provided with circular holes. A glass funnel is inserted into the inside of the circular holes. The bottom surface of the glass funnel is provided with a liquid outlet pipe. The inside of the glass funnel is provided with a filter assembly. The top surfaces of the first base and the second base are provided with an identification unit. This also includes a valve, which is installed on the outside of the outlet pipe.

[0005] The top surfaces of both the first and second bases have 20 round holes for processing different batches of samples. The glass funnel is installed inside the round hole, and pure water is added. After 2-3 hours, the pine wood nematode sample is separated. Finally, the valve is pressed to discharge 5-15 ml of water sample from the bottom of the glass funnel for subsequent experiments.

[0006] Furthermore, it also includes a housing and a temperature control device. The housing is disposed around the first base and the second base, completely enclosing the first base and the second base. The housing is provided to prevent heat loss, thereby shortening the heating time.

[0007] Furthermore, the filter assembly includes a rotating wheel, a fixed column, a pressure plate, a screw, and a filter screen. The filter screen is fixed inside the glass funnel. A screw is rotatably connected to the center of the top surface of the filter screen. The screw is threadedly connected to a screw hole in the center of the pressure plate. A fixed column is installed on the top surface of the screw. The fixed column is inserted into a fixed groove in the center of the rotating wheel. The fixed groove in the rotating wheel is engaged with the outside of the fixed column. Rotating the rotating wheel drives the screw to rotate, thereby pressing the pressure plate down on the pine nematode sample. The filter screen can prevent the pine nematode sample from clogging the glass funnel.

[0008] Furthermore, the identification unit includes a fixing frame, an information card, a placement slot, an insert, and a slot. The slots are evenly distributed on the rear side of the top surface of the first base and the second base. An insert is fixed to the bottom surface of the fixing frame, and the insert is inserted into the slot. Placement slots are provided on both the upper and lower sides inside the fixing frame. An information card is slidably installed inside the placement slot. The insert is inserted into the slot to install the fixing frame. The information card inside the placement slot can record information about the pine nematode wood sample inside the glass funnel for easy viewing by personnel.

[0009] Furthermore, the diameter of the circular holes is 8cm, and the distance between multiple circular holes is 6cm, which facilitates the handling of multiple glass funnels.

[0010] Furthermore, the glass funnel has a height of 10cm, a waist diameter of 8cm, and a top diameter of 10cm, and its large capacity facilitates the placement of larger pine nematode wood samples.

[0011] Furthermore, it also includes timers, two of which are respectively installed on the top left side of the first base and the second base. The timers are set to remind personnel to check when the separation is completed.

[0012] Furthermore, the filter assembly includes a filter basket and a handle. The filter basket is placed inside the glass funnel, and the top surface of the filter basket is fixed with a handle. When in use, the sample wood block is wrapped in gauze or non-woven fabric and placed in the filter basket to prevent sawdust from the pine wood nematode sample from clogging the glass funnel. The handle makes it easy to pick up the filter basket.

[0013] Furthermore, the temperature control device includes a microcontroller, a relay, an electric blanket, a temperature sensor, and a power supply. The microcontroller and relay are fixed to the back of the second base. The microcontroller is electrically connected to the relay, electric blanket, temperature sensor, and power supply. The electric blanket is fixed to the inside of the outer shell for heating the glass funnel. At least two temperature sensors are provided, respectively fixed to the inner sidewalls of the first and second bases. The temperature control device is used to control the heating temperature, on the one hand to prevent the temperature from being too high and causing accidents, and on the other hand to keep the temperature inside the glass funnel below or equal to the preset maximum temperature. This can both accelerate the separation of pine wood nematodes and prevent the pine wood nematodes from being scalded to death. The advantage of using an electric blanket for heating is that it is lightweight and easy to fix and disassemble, has low power consumption, and the maximum heating temperature is about 50 to 60 degrees Celsius. Therefore, even if the temperature control device malfunctions, it is not likely to cause accidents.

[0014] Compared with the prior art, the beneficial effects of the present invention are as follows: This apparatus for batch separation of pine wood nematode samples has the following advantages: 1. The 20 round holes on the top surfaces of the first and second bases can be used to process different batches of samples. The glass funnel is installed inside the round holes, and pure water is added. After 2-3 hours, the pine wood nematode sample is separated. Finally, the valve is pressed to discharge 5-15 ml of water sample from the bottom of the glass funnel for subsequent experiments.

[0015] 2. The rotating wheel is engaged with the outside of the fixed column through the fixing groove in the rotating wheel. Rotating the rotating wheel drives the screw to rotate, which presses the pressure plate down on the pine wood nematode wood sample. The filter screen can prevent the pine wood nematode wood sample from clogging the glass funnel.

[0016] 3. The mounting bracket is installed by inserting the insert block into the slot. The information card placed inside the slot can record information about the pine nematode wood sample inside the glass funnel for easy viewing by personnel. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of the present invention; Figure 2 This is a schematic diagram of the filter component structure of the present invention; Figure 3 This is a schematic diagram of the identification unit structure of the present invention; Figure 4This is a schematic diagram of another filter component structure according to the present invention; Figure 5 A schematic diagram of the temperature control device of the present invention.

[0018] In the diagram: 1 First base, 2 Filter assembly, 21 Rotary wheel, 22 Fixed column, 23 Pressure plate, 24 Screw, 25 Filter screen, 3 Identification unit, 31 Fixing frame, 32 Information card, 33 Placement slot, 34 Insert block, 35 Slot, 4 Second base, 5 Partition plate, 6 Round hole, 7 Glass funnel, 8 Liquid outlet pipe, 9 Valve, 10 Timer, 11 Basket, 12 Handle. Detailed Implementation

[0019] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0020] Please see Figure 1-5 This embodiment provides a technical solution: a device for batch separation of pine wood nematode samples, including a first base 1 and a partition 5; First base 1: A second base 4 is fixedly installed on the rear side. The height of the first base 1 is 0.25m, and the height of the second base 4 is 0.6m. The first base 1 and the second base 4 are combined in a stepped manner. Partition plates 5 are evenly fixed inside the first base 1 and the second base 4. Circular holes 6 are evenly opened on the top surface of the first base 1 and the second base 4. A glass funnel 7 is inserted into the circular hole 6. The bottom surface of the glass funnel 7 is provided with a liquid outlet pipe 8. A filter assembly 2 is provided inside the glass funnel 7. The filter assembly 2 includes a rotating wheel 21, a fixing column 22, a pressure plate 23, a screw 24, and a filter screen 25. The filter screen 25 is fixed inside the glass funnel 7. The screw 24 is rotatably connected to the middle of the top surface of the filter screen 25. The screw 24 is threadedly connected to the screw hole in the middle of the pressure plate 23. The fixing column 22 is installed on the top surface of the screw 24. The fixing column 22 is inserted into the fixing groove in the middle of the rotating wheel 21. The fixing groove in the rotating wheel 21 is engaged with the fixing column 22. On the outside of column 22, rotating wheel 21 drives screw 24 to rotate to press the pressure plate 23 down on the pine nematode wood sample. The filter screen 25 can prevent the pine nematode wood sample from clogging the glass funnel 7. The top surface of the first base 1 and the second base 4 is provided with identification unit 3. The identification unit 3 includes a fixing frame 31, information card 32, placement groove 33, insert block 34 and slot 35. The slots 35 are evenly opened on the rear side of the top surface of the first base 1 and the second base 4. The bottom surface of the fixing frame 31 is fixed with insert block 34, which is inserted into the slot 35. The upper and lower sides of the inside of the fixing frame 31 are provided with placement groove 33. The information card 32 is slidably installed inside the placement groove 33. The insert block 34 is inserted into the inside of the slot 35 to install the fixing frame 31. The information card 32 inside the placement groove 33 can record the information of the pine nematode wood sample inside the glass funnel 7 for easy viewing by personnel. The system also includes a valve 9, which is installed on the outside of the outlet pipe 8. The top surfaces of the first base 1 and the second base 4 each have 20 round holes 6 for processing different batches of samples. A glass funnel 7 is installed inside the round hole 6, and pure water is added. After 2-3 hours, the pine wood nematode sample is separated. Finally, the valve 9 is pressed to discharge 5-15 ml of water from the bottom of the glass funnel 7 for subsequent experiments. The diameter of the round hole 6 is 8 cm, and the spacing between multiple round holes 6 is 6 cm, facilitating the handling of multiple glass funnels 7. The height of the glass funnel 7 is 10 cm, the waist diameter is 8 cm, and the top diameter is 10 cm. The large capacity allows for the placement of larger pine wood nematode samples. The system also includes a timer 10, with two timers installed on the top left side of the first base 1 and the second base 4 respectively. The timers 10 are designed to remind personnel to check when the separation is complete. The filter assembly 2 includes a filter basket 11 and a handle 12. The filter basket 11 is placed inside the glass funnel 7. The handle 12 is fixed on the top surface of the filter basket 11. When in use, the sample wood block is wrapped in gauze or non-woven fabric and placed in the filter basket 11 to prevent sawdust from the pine wood nematode sample from clogging the glass funnel 7. The handle 12 makes it easy to pick up the filter basket 11.

[0021] This embodiment describes a device for batch separation of pine wood nematode samples, further comprising an outer shell and a temperature control device. The outer shell is disposed around a first base and a second base, completely enclosing the first and second bases. The outer shell is used to prevent heat loss, thereby shortening the heating time. The temperature control device includes a microcontroller, a relay, an electric blanket, a temperature sensor, and a power supply. The microcontroller and relay are fixed to the back of the second base. The microcontroller is electrically connected to the relay, electric blanket, temperature sensor, and power supply. The microcontroller uses an AT89C51 microcontroller, the temperature sensor uses a DS18B20 microcontroller, and the relay uses a Chint miniature intermediate relay NXJ / 2ZH4Z(D). The electric blanket is fixed to the inside of the outer shell for heating the glass funnel. At least two temperature sensors are provided, fixed to the inner side walls of the first and second bases respectively. The temperature control device is used to control the heating temperature, preventing accidents caused by excessive temperature and maintaining the temperature inside the glass funnel below or equal to the preset maximum temperature. This accelerates the separation of pine wood nematodes while preventing them from being scalded to death. The advantage of using an electric blanket for heating is that it is lightweight, easy to fix and disassemble, has low power consumption, and the maximum heating temperature is approximately 50 to 60 degrees Celsius. Therefore, even if the temperature control device malfunctions, it is unlikely to cause accidents.

[0022] In this embodiment, in order to improve the safety performance of a device for batch separation of pine wood nematode samples, the temperature control device also includes an alarm module, a display device, and a button module. The alarm module uses a buzzer, which starts to work when the temperature inside the casing is higher than the preset maximum temperature.

[0023] The entire system uses the AT89C51 microcontroller as its core component, along with resistors, capacitors, and a crystal oscillator, forming the minimum microcontroller-based system. Other modules revolve around the microcontroller. These include a DS18B20 temperature sensor responsible for collecting temperature data and sending it to the microcontroller; a 4-digit common-cathode LED display showing the detected temperature value; and a button module primarily for setting alarm thresholds. The alarm module uses a buzzer system; if the temperature exceeds the set range, an alarm is triggered, and the microcontroller simultaneously controls a relay to power on or off, keeping the temperature within the set range.

[0024] The working principle of the device for batch separation of pine wood nematode wood samples provided by the present invention is as follows: First, a suitable number of glass funnels 7 are installed inside the round holes 6. The top surfaces of the first base 1 and the second base 4 each have 20 round holes 6 to process different batches of samples. The pine wood nematode wood sample is placed inside the glass funnel 7. The fixing groove in the rotating wheel 21 is engaged with the outside of the fixing column 22. Rotating the rotating wheel 21 drives the screw 24 to rotate, pressing the pressure plate 23 down on the pine wood nematode wood sample. The filter screen 25 prevents the pine wood nematode wood sample from clogging the glass funnel 7. Simultaneously, pure water is added and left for 2-3 hours to separate the pine wood nematode. The wood samples are separated, and finally, valve 9 is pressed to drain 5-15ml of water from the bottom of the glass funnel 7 for subsequent experiments. At the same time, the sample wood block can be wrapped in gauze or non-woven fabric and placed in the basket 11 to prevent sawdust from the pine nematode wood sample from clogging the glass funnel 7. The handle 12 makes it easy to pick up the basket 11, and the insert 34 is inserted into the slot 35 to install the fixing frame 31. The information card 32 inside the slot 33 can record the information of the pine nematode wood sample inside the glass funnel 7. The timer 10 is set up to remind personnel to check when the separation is completed. In order to accelerate the separation of pine nematodes, an outer shell is set on the outside of the pine nematode wood sample batch separation device. A temperature control device is set inside the outer shell to heat the glass funnel, thereby raising the water temperature inside the glass funnel and accelerating the separation of pine nematodes.

[0025] The above description is merely an embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural or procedural transformations made based on the content of the present invention specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of the present invention.

Claims

1. A device for bulk isolation of pinewood nematode wood, characterized by: It include first base (1) and partition (5); First base (1): the rear side is fixedly installed with second base (4), the partition (5) is uniformly fixed in the inside of first base (1) and second base (4), the top surface of first base (1) and second base (4) is uniformly provided with round hole (6), the inside of round hole (6) is inserted with glass funnel (7), the bottom surface of glass funnel (7) is provided with liquid outlet pipe (8), the inside of glass funnel (7) is provided with filter assembly (2), the top surface of first base (1) and second base (4) is provided with identification unit (3); It also includes valve (9), which is installed on the outside of liquid outlet pipe (8).

2. The device for bulk isolation of pine wood nematode wood samples according to claim 1, wherein: It also includes a shell and a temperature control device, the shell is arranged on the periphery of the first base and the second base, and the first base and the second base are completely covered in the shell.

3. The device for bulk isolation of pinewood nematode wood according to claim 1, wherein: The filter assembly (2) includes a rotating wheel (21), a fixed column (22), a pressing plate (23), a screw rod (24) and a filter screen (25), the filter screen (25) is fixed in the inside of the glass funnel (7), the top surface of the filter screen (25) is rotatably connected with the screw rod (24) in the middle, the screw rod (24) is threadedly connected with the screw hole in the middle of the pressing plate (23), the top surface of the screw rod (24) is provided with the fixed column (22), and the fixed column (22) is correspondingly inserted into the fixed groove in the middle of the rotating wheel (21).

4. The device for bulk isolation of pine wood nematode wood samples according to claim 1, wherein: The identification unit (3) includes a fixed frame (31), an information card (32), a placing groove (33), an insertion block (34) and an insertion slot (35), the insertion slot (35) is uniformly provided on the rear side of the top surface of the first base (1) and the second base (4), the bottom surface of the fixed frame (31) is fixedly provided with the insertion block (34), the insertion block (34) is correspondingly inserted into the insertion slot (35), and the placing groove (33) is formed in the inside of the fixed frame (31) on the upper side and the lower side. The information card (32) is slidably installed in the inside of the placing groove (33).

5. The device for bulk isolation of pinewood nematode wood according to claim 1, wherein: The diameter of the round hole (6) is 8cm, and the spacing between the plurality of round holes (6) is 6cm.

6. The device for bulk isolation of pinewood nematode wood according to claim 1, wherein: The height of the glass funnel (7) is 10cm, the waist diameter of the glass funnel (7) is 8cm, and the top diameter of the glass funnel (7) is 10cm.

7. The device for bulk isolation of pinewood nematode wood according to claim 1, wherein: It also includes a timer (10), and the timer (10) has two and is installed at the top of the left side of the first base (1) and the second base (4) respectively.

8. The device for bulk isolation of pinewood nematode wood according to claim 1, wherein: The height of the first base (1) is 0.25m, the height of the second base (4) is 0.6m, and the first base (1) and the second base (4) are combined into a stepped type.

9. The device for bulk isolation of pinewood nematode wood according to claim 1, wherein: The filter assembly (2) includes a filter basket (11) and a handle (12), the filter basket (11) is placed in the inside of the glass funnel (7), and the handle (12) is fixed to the top surface of the filter basket (11).

10. The device for bulk isolation of pinewood nematode wood according to claim 2, wherein: The temperature control device comprises a single-chip microcomputer, a relay, an electric blanket, a temperature sensor and a power supply, the single-chip microcomputer and the relay are fixed on the back of the second base, the single-chip microcomputer is electrically connected with the relay, the electric blanket, the temperature sensor and the power supply, the electric blanket is fixed on the inner side of the shell for heating the glass funnel, and the temperature sensor is provided with at least two and is fixed on the side walls in the interiors of the first base and the second base.

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