Imitation wild cultivation method for cremastra appendiculata

Abstract

The invention provides a wild-imitating cultivation method for cremastra appendiculata, and belongs to the technical field of orchid plant cultivation. The method comprises the specific steps that wood blocks are treated and laid, and water numbness wood blocks are laid on ridge surfaces; seed treatment and sowing, wherein seeds and wet debregeasia edulis wood chips are mixed and then sown; spreading rotted leaves, namely spreading dried beancurd stick leaves after sowing; and field management. Wherein the cremastra appendiculata can be cultivated in a potting or land mode. According to the wild-imitating cultivation method for the cremastra appendiculata provided by the invention, the water numbness wood blocks and the dried beancurd stick leaves are provided to construct the bionic microhabitat, the water numbness wood blocks provide a natural host environment for symbiotic fungi, the dried beancurd stick leaves release rich organic matters after being decomposed and maintain soil humidity, and efficient planting is realized through the construction of the bionic microhabitat without additional associated fungus bags; the cultivation cost is obviously reduced.

Description

Technical Field

[0001] This invention belongs to the field of orchid cultivation technology, specifically relating to a method for semi-wild cultivation of *Cymbidium goeringii*. Background Technology

[0002] As an important member of the Orchidaceae family, *Sagittaria sagittifolia* exhibits high value in both medicinal and ornamental fields. Its dried pseudobulbs are used medicinally; they are sweet and slightly pungent, cool in nature, and slightly toxic. They possess significant effects such as clearing heat and detoxifying, resolving phlegm and dissipating nodules, and reducing swelling. They play a crucial role in the treatment of carbuncles, boils, scrofula, lymph node tuberculosis, and snake and insect bites. They are also attracting attention in the development of anti-tumor drugs. From an ornamental perspective, *Sagittaria sagittifolia* has an elegant form and unique flowers, making its ornamental value considerable.

[0003] However, *Cistanche deserticola* faces a severe survival and supply dilemma. In its natural environment, its reserves are already scarce, its growth is slow, and it has demanding ecological requirements, mostly distributed in specific shady, well-drained mountainous areas. However, the strong market demand for *Cistanche deserticola*, driven by high profits, has led to the disorderly and excessive harvesting of wild resources. The large-scale excavation of wild *Cistanche deserticola* has severely damaged its growing environment, drastically reduced its population, and brought wild resources to the brink of depletion. It has been listed as a national key protected wild plant, and its survival is extremely precarious.

[0004] Currently, the cultivation of hairy sesame is mainly done artificially. However, the existing cultivation technology has a low land utilization rate, and the survival rate of plants is low and their growth is poor.

[0005] Patent application CN117652342A discloses a method for cultivating seedlings of the traditional Chinese medicinal herb *Cistanche deserticola*, specifically including steps such as ridging, spreading *Cistanche deserticola* accompanying mycelium, laying miscellaneous tree leaves and branches, sowing seeds, covering with humus soil, covering with pine needles, and erecting a shade net. However, this method requires the use of *Cistanche deserticola* accompanying mycelium bags, which are expensive to prepare; furthermore, this method requires spreading accompanying mycelium granules, resulting in high labor and material input costs. Summary of the Invention

[0006] To address the problems existing in the prior art, this invention provides a method for the simulated wild cultivation of *Cymbidium goeringii*, thereby solving the problems mentioned in the background art.

[0007] This invention provides a method for semi-wild cultivation of *Sagittaria sagittifolia*, the method comprising the following steps: (1) Wood block treatment and laying: Water hemp wood blocks are laid on the ridge surface; (2) Seed treatment and sowing: Mix the seeds with moistened hemp fiber and then sow them; (3) Spreading decomposed leaves: Spread decomposed bamboo leaves after sowing; (4) Field management.

[0008] Furthermore, the cultivation method of the hairy arrowhead mushroom is pot cultivation, preferably cultivation in a flowerpot with a diameter of 30cm.

[0009] Furthermore, the cultivation method of the hairy shiitake mushroom is land cultivation. Before step (1), there is also a land preparation step, which is specifically carried out as follows: after removing weeds and stones from the land, deep plowing, ridging, and soil disinfection are performed.

[0010] Furthermore, the specific operation of deep plowing after clearing weeds and stones from the land is as follows: Select a mountainous area with a slope of 30-45 degrees and a pH value of 5.5-6.5, clear weeds and stones, plow the soil to a depth of 20-30 cm, let the soil dry for 5-7 days, apply base fertilizer, and dig drainage ditches. The specific operation of ridging is as follows: ridging is carried out according to the terrain, the ridging width is controlled at 1.2~1.5m, the ridging height is set at 20~25cm, the width of the furrow between the ridges is 40~50cm, and the depth of the furrow between the ridges is 30~35cm; The specific operation of soil disinfection is as follows: Apply 50-100 kg of quicklime per mu of land. First, plow and harrow the soil, then spread the lime evenly and plow it again to mix the lime thoroughly with the soil. During use, the lime should not be mixed with acidic fertilizers or pesticides.

[0011] Furthermore, the specific operation of the wood block processing and laying is as follows: Select fresh water hemp wood and process it into wood blocks that are 5-8cm long, 3-5cm wide, and 2-3cm thick; lay the processed wood blocks in two to three layers on the ridge, with each layer arranged in an alternating manner, and the wood blocks between layers are placed vertically, with a total laying thickness of 3-10cm.

[0012] Furthermore, the total thickness of the paving is 5-10 cm.

[0013] Furthermore, the specific operations for seed treatment and sowing are as follows: Select mature, plump, and disease-free *Sagittaria sagittifolia* pods, and air-dry them in the shade. The thickness of the air-drying should not exceed 2 cm, and the time should be 7-10 days. Peel open the treated pods, and thoroughly mix the seeds inside the pods with moistened *Hydrocotyle vulgaris* shavings at a mass ratio of 1:30-1:70. Sow the mixture evenly on the ridge surface at a density of 1-1.5 pods per square meter.

[0014] Furthermore, the mass ratio of the seeds to the moistened hemp fiber is 1:50; the density is 1.5 dried pods per square meter.

[0015] Furthermore, the specific operation of spreading the decomposed leaves is as follows: spread a 3-12cm thick layer of decomposed bamboo leaves on the surface of the ridge after sowing.

[0016] Furthermore, the specific operations of the field management include: water management, temperature and light control, weed removal, thinning and fixing seedlings, applying fertilizer, and pest and disease control; In this process, the humidity is maintained at 70% to 85% during water management; the temperature is maintained at 15 to 25℃; and the spacing between seedlings is 10 to 15 cm during thinning. The fertilizer is organic fertilizer, preferably sheep manure; the density of the added fertilizer is 600 kg / mu to 800 kg / mu. The pesticides used for pest and disease control are selected from metaldehyde, pyridaben, phoxim, or imidacloprid.

[0017] Furthermore, the specific operations of the field management are as follows: 1) Water management: Adjust the watering method according to the season and the growth stage of Sagittaria trifolia, and follow the principle of "mainly spraying, spraying frequently but in small amounts" to keep the substrate surface moist but not waterlogged; spray every morning and evening during the high temperature period, spray water once every 3 to 5 days during the low temperature period, and appropriately increase the amount of watering during the bulb enlargement period of Sagittaria trifolia. 2) Temperature and light control: Maintain the growing environment temperature at 15~25℃, build a 70% shade net in summer, and cover the ridge surface with 5cm thick water hemp wood chips and film for insulation in winter. 3) Weed removal: Weeds should be removed by hand. Avoid using hoes or mechanical weeding during the seedling stage. Weed every 15 to 20 days, depending on the growth of the weeds. 4) Thinning and fixing seedlings: When the seedlings grow to 5-8cm in height, thin them according to the standard of 10-15cm between plants and 20-25cm between rows. Remove weak seedlings, diseased seedlings and overly dense seedlings, and fill in the gaps in time. 5) Add sheep manure organic fertilizer: Combined with the base fertilizer during land preparation, it forms a full-cycle supply of organic nutrients, promoting root expansion and bulb development; 6) Pest and disease control: Regularly inspect the field and apply pesticides to control any pests and diseases found.

[0018] Furthermore, the following pesticide application methods are used for the prevention and control of the aforementioned diseases and pests: If snails are found gnawing, apply 400-500g of 6% metaldehyde granules evenly per acre, spreading it evenly on the surface and surrounding area of ​​the seedbed, avoiding direct contact between the pesticide and the *Sagittaria sagittifolia* plants; if cutworm (noctuid moth) symptoms are present, they bite the stems and leaves, leading to missing seedlings and broken rows, use a cutworm insecticide for root irrigation; if spider mite symptoms are present, the leaves will show yellow-white spots, use abamectin for spraying control at the initial stage of the disease; if grub (scarab beetle larvae) symptoms are present, they will eat the plant's young roots, tender stems, and tubers, causing stunted plant growth, yellowing leaves, poor fruit development, and in severe cases, even plant death, use phorate granules to control them before planting; aphid symptoms include curled and yellowed leaves, stunted growth, poor plant development, and in severe cases, withered branches and leaves, leading to the death of the entire plant. Spray with a 10% imidacloprid emulsion at a dilution of 1000-1500 times for control.

[0019] The present invention has achieved the following beneficial effects: (1) This invention improves the soil structure and drainage performance of mountainous areas by ridge-making on slopes of 30°~45° and laying three-dimensional substrates, combined with the layered laying of three-dimensional substrates (such as sawdust, humus, straw, etc.), and constructs multi-layered planting micro-domains in a limited space, thereby improving land utilization and adapting to the development of mountainous spaces. This invention proposes to construct a biomimetic micro-environment using water hemp wood blocks and dried bamboo leaves. Water hemp wood blocks provide a natural host environment for symbiotic fungi, and dried bamboo leaves release rich organic matter after decomposition and maintain soil moisture, significantly improving seedling survival rate and promoting robust plant growth. This invention forms a full-cycle organic nutrient supply by combining base fertilizer during land preparation with topdressing with sheep manure after seedling emergence, thereby increasing yield and enhancing the effective components of medicinal materials.

[0020] (2) The water hemp wood blocks used in this invention have the following specific advantages: The three-dimensional structure of the water hemp wood blocks (2-3 layers of vertical interlacing) provides a suitable colonization environment for symbiotic fungi. The tannins, cellulose and other substances released during the decomposition of its wood fibers can induce symbiotic fungi (such as the genus *Mammillaria*) of orchid plants to colonize, forming mycorrhizae to assist seed germination. In contrast, substrates such as coconut shells and pine bark lack similar wood structures or components and cannot support the establishment of fungal communities, resulting in seeds being unable to break dormancy.

[0021] (3) Compared with the method disclosed in patent application CN117652342A, this invention confirms that the use of water-hemp wood blocks can replace the role of "symbiotic fungal bags," achieving germination through natural induction of symbiotic fungi, and at a lower cost (saving the cost of preparing fungal bags). Although the artificially laid substrates such as coconut shells used in the comparative example have similar structures, they failed to germinate because they did not carry natural symbiotic fungi, indirectly proving the scientific validity of the "wood block-induced fungi" scheme of this invention.

[0022] (4) Compared with patent application CN117652342A, the method of the present invention does not require the sowing of associated bacteria particles, reducing the input of labor and materials; combined with ridge making on slopes and leaf covering, a micro-ecology close to the wild environment is constructed, which not only improves the survival rate of seedlings and the yield per mu, but also ensures that the effective components of medicinal materials meet the standards, thus realizing low-cost and sustainable large-scale cultivation.

[0023] Obviously, based on the above description of the present invention, and according to common technical knowledge and conventional methods in the field, various other modifications, substitutions or alterations can be made without departing from the basic technical concept of the present invention.

[0024] The following detailed embodiments further illustrate the above-described content of the present invention. However, this should not be construed as limiting the scope of the present invention to the following embodiments. All technologies implemented based on the above-described content of the present invention fall within the scope of the present invention. Attached Figure Description

[0025] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0026] Figure 1 This is a schematic flowchart of a method for simulating wild cultivation of *Cymbidium goeringii* provided in an embodiment of the present invention.

[0027] Figure 2 This is a seedling image of *Cistanche deserticola* provided in an embodiment of the present invention.

[0028] Figure 3 This invention provides a method for the simulated wild cultivation of Sagittaria trifolia under different cultivation conditions, as described in Example 1 and Comparative Examples 1-4 of the present invention.

[0029] Figure 4 The protocorms generated from the germination of Sagittaria trifolia seeds after 120 days of cultivation in Example 1. Detailed Implementation

[0030] The following specific embodiments illustrate the implementation of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification.

[0031] It should be noted that the term "comprising" or any other variation is intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0032] according to Figure 1 The process shown is used to simulate wild cultivation of Sagittaria sagittifolia, and the results are obtained. Figure 2 Seedlings of *Sagittaria sagittifolia* are shown.

[0033] The following precautions should be taken in the embodiments of this invention: After cultivating *Saussurea involucrata*, in the early stages, to maintain warmth and moisture, evenly cover the soil surface with a 3-12cm thick layer of well-rotted bamboo leaves. The natural porous structure of the leaves helps maintain substrate moisture and mitigates diurnal temperature fluctuations. Once temperatures rise in May, retain the leaf cover layer (adjusting the thickness to 3-5cm) according to the seedling condition, and construct a shade net (50%-70% shading rate) on the raised beds. This provides diffused light for the seedlings and, through the combined effect of the leaves and the shade net, prevents rainwater from directly washing away the raised beds, which could cause seed displacement or seedling lodging.

[0034] During the growing season, the humidity of the leaf mold covering layer needs to be continuously monitored to keep the substrate moist (it should clump together when squeezed by hand). If drought occurs during the sowing period (March-April), water can be slowly poured along the furrows between rows or a mist sprayer can be used to lightly spray, avoiding water flow from impacting the leaf mold layer. During the flowering period (May-June) and fruiting period (September-December), special attention should be paid to soil moisture. In hot seasons, a layer of pine needles can be spread on the surface of the leaf mold to further enhance water retention.

[0035] Before the rainy season, dig a circular drainage ditch 30-40cm deep and 25-30cm wide around the perimeter of the cultivation area, and compact the ditch walls to prevent collapse. In case of heavy rain or torrential rain, a breathable rain-proof net can be temporarily placed over the shade net to reduce the penetration and erosion of the leaf mold layer by rainwater. Remove the rain-proof net promptly after the rain to prevent seedling suffocation. In daily management, gently turn over the leaf mold layer while weeding to promote air circulation, while preventing people and animals from entering the ridges and trampling on the leaf mold structure to maintain the stability of the microhabitat.

[0036] Example 1: Semi-wild cultivation method of Sagittaria sagittifolia Cultivation conditions screening: 30cm diameter flowerpots were used for cultivation, and a control experiment was conducted.

[0037] (1) Mixed soil laying: peat soil and vermiculite are mixed in a 2:1 ratio. (2) Wood block spreading: Three layers of water-hemp wood blocks (7cm long × 5cm wide × 3cm thick) are laid, with vertical and three-dimensional interlacing between the layers, and a total thickness of 5cm, forming a three-dimensional rotten wood layer on the ridge surface.

[0038] (3) Seed sowing: After the seeds have been air-dried for 10 days, they are mixed with sawdust at a mass ratio of 1:50. Sow 1.5 pods per square meter and then lightly cover with 1cm of soil.

[0039] (4) Spreading decomposed leaves: Mix decomposed bamboo leaves with pine needles and cover with a 12cm thick layer.

[0040] (5) Field management: Water every 4 days.

[0041] Comparative Example 1: Semi-wild Cultivation Method of *Sagittaria sagittifolia* Cultivation conditions screening: 30cm diameter flowerpots were used for cultivation, and a control experiment was conducted.

[0042] (1) Mixed soil laying: peat soil and vermiculite are mixed in a 2:1 ratio. (2) Spreading coconut shells: The water coconut shells are spread densely with a total thickness of 5cm.

[0043] (3) Seed sowing: After the seeds have been air-dried for 10 days, they are mixed with sawdust at a mass ratio of 1:50. Sow 1.5 pods per square meter and then lightly cover with 1cm of soil.

[0044] (4) Spreading decomposed leaves: Mix decomposed bamboo leaves with pine needles and cover with a 12cm thick layer.

[0045] (5) Field management: Water every 4 days.

[0046] Comparative Example 2: Semi-wild Cultivation Method of *Sagittaria sagittifolia* Cultivation conditions screening: 30cm diameter flowerpots were used for cultivation, and a control experiment was conducted.

[0047] (1) Mixed soil laying: peat soil and vermiculite are mixed in a 2:1 ratio. (2) Pine bark spreading: Pine bark is spread densely with a total thickness of 5cm.

[0048] (3) Seed sowing: After the seeds have been air-dried for 10 days, they are mixed with sawdust at a mass ratio of 1:50. Sow 1.5 pods per square meter and then lightly cover with 1cm of soil.

[0049] (4) Spreading decomposed leaves: Mix decomposed bamboo leaves with pine needles and cover with a 12cm thick layer.

[0050] (5) Field management: Water every 4 days.

[0051] Comparative Example 3: Semi-wild Cultivation Method of *Sagittaria sagittifolia* Cultivation conditions screening: 30cm diameter flowerpots were used for cultivation, and a control experiment was conducted.

[0052] (1) Mixed soil laying: peat soil and vermiculite are mixed in a 2:1 ratio. (2) Corn cob spreading: The corn cobs are spread densely with a total thickness of 5cm.

[0053] (3) Seed sowing: After the seeds have been air-dried for 10 days, they are mixed with sawdust at a mass ratio of 1:50. Sow 1.5 pods per square meter and then lightly cover with 1cm of soil.

[0054] (4) Spreading decomposed leaves: Mix decomposed bamboo leaves with pine needles and cover with a 12cm thick layer.

[0055] (5) Field management: Water every 4 days.

[0056] Comparative Example 4: Semi-wild Cultivation Method of *Sagittaria sagittifolia* Cultivation conditions screening: 30cm diameter flowerpots were used for cultivation, and a control experiment was conducted.

[0057] (1) Mixed soil laying: peat soil and vermiculite are mixed in a 2:1 ratio. (2) Spreading coconut coir: The coconut coir is spread densely with a total thickness of 5cm.

[0058] (3) Seed sowing: After the seeds have been air-dried for 10 days, they are mixed with sawdust at a mass ratio of 1:50. Sow 1.5 pods per square meter and then lightly cover with 1cm of soil.

[0059] (4) Spreading decomposed leaves: Mix decomposed bamboo leaves with pine needles and cover with a 12cm thick layer.

[0060] (5) Field management: Water every 4 days.

[0061] According to the cultivation conditions of Example 1 and Comparative Examples 1-4, three pots were prepared for each example. Figure 3 Seeds of *Sagittaria sagittifolia* cultivated for 120 days under the cultivation conditions of Example 1 and Comparative Examples 1-4 were compared. Figure 4 ).

[0062] Example 1: Seeds germinate and form "dragon eggs" (bulbs of hairy arrowhead seedlings), with a germination rate of about 54% and a bulb diameter of 0.5-0.8 cm. Fungal hyphae in the gap between the roots and the wood block form a distinct symbiotic structure.

[0063] Comparative Examples 1-4: No seed germination was observed, no seedlings emerged from the substrate surface, and dissection revealed that the seeds had not broken through the seed coat and some were moldy.

[0064] The results show that under the conditions of Example 1, the seeds were able to successfully germinate and form bulbils of *Sagittaria sagittifolia* seedlings, and formed a good symbiotic structure with the fungus; while under the conditions of Comparative Examples 1-4, the seeds failed to germinate, and some even became moldy. This indicates that in Example 1, laying water-hemp wood blocks on the ridge surface was necessary to induce seed germination and seedling growth, while laying water coconut husks, pine bark, corn cobs, and coconut coir on the ridge surface in Comparative Examples 1-4 failed to induce seed germination.

[0065] Based on the above potted cultivation experiments, this invention further demonstrates large-scale planting in shady mountainous areas at 30-45° elevations. The difference between potted cultivation (Example 1) and large-scale planting in mountainous areas (Examples 2-4) lies in: Planting scenarios: Example 1 used a 30cm diameter flowerpot as a control experiment, while Examples 2-4 were large-scale plantings in shady mountainous areas at 30-45° angles. Differences in steps: Example 1 adds "laying a mixture of peat moss and vermiculite (2:1)", while it lacks "turning the soil, sun-drying it, ridging it, and disinfecting it" as described in Examples 2-4; Management differences: Example 1 only involves "watering every 4 days", while Examples 2-4 involve more detailed management such as seasonal watering, applying sheep manure, preventing diseases and pests, and manual weeding.

[0066] Comparison of the two: Example 1 shows that "water hemp block can induce seed germination" (compared to Comparative Examples 1-4, only it produced seedlings), providing principle support for the mountain planting schemes of Examples 2-4; Examples 2-4 are mountain-scale implementation schemes based on this principle.

[0067] Example 2: Semi-wild cultivation method for *Sagittaria sagittifolia* (1) Deep plowing: Select steep slopes (35°-45°), manually remove weeds and stones, then mechanically plow to a depth of 20cm and sun-dry the soil for 5 days to kill insect eggs.

[0068] (2) Ridging: Ridges are made along the contour lines. The ridge width is 1.2m (including the ditch), the ridge height is 20cm, the ditch width between ridges is 40cm, the ridge surface is flat, and the ditch depth is 30cm to facilitate drainage.

[0069] (3) Wood block spreading: Two layers of dry water-soaked hemp wood blocks (5cm long × 3cm wide × 2cm thick) are laid, with the layers arranged vertically between them, with a total thickness of 5cm, covering 80% of the ridge surface area.

[0070] (4) Seed sowing: In autumn, the seeds are dried in the shade for 7 days, mixed with moist sawdust at a mass ratio of 1:50, and one seed pod is evenly sown per square meter in the gaps between the wood blocks.

[0071] (5) Spreading decomposed leaves: Cover with 5cm thick decomposed bamboo leaves and press lightly to make the seeds contact the substrate.

[0072] (6) Field management: Moisture: Spray once a day in summer and every 3 days in winter to maintain humidity at 70%; Thinning: When the seedlings are 5cm tall, thin them to a spacing of 10cm between plants. Weed manually once every 15 days. Insect control: When snails cause damage, apply 400g / acre of 6% metaldehyde granules.

[0073] Example 3: Semi-wild cultivation method of Sagittaria sagittifolia (1) Deep plowing: Select steep slopes (35°-45°), plow mechanically to a depth of 25cm, and then expose the soil to the sun for 6 days.

[0074] (2) Ridging of whole boxes: ridge width 1.3m (including ditch), ridge height 22cm, ditch width between ridges 45cm, ridge surface slightly arched, drainage ditch depth 33cm to prevent water accumulation.

[0075] (3) Wood block spreading: Two layers of water hemp wood blocks (6cm long × 4cm wide × 3cm thick) are laid, with vertical spacing between the layers and the gaps filled with dried bamboo leaves, for a total thickness of 7cm, covering 90% of the ridge surface.

[0076] (4) Seed sowing: The seeds are dried in the shade for 8 days, mixed with sawdust at a mass ratio of 1:50, and then covered with mulch for 5 days to keep them moist. Sow 1.2 seed pods per square meter in the gaps between the wood blocks.

[0077] (5) Spreading decomposed leaves: After sieving, the decomposed leaves are evenly covered with an 8cm thick layer, with the edges extending 10cm beyond the ridge surface to prevent erosion.

[0078] (6) Field management: Moisture: Alternate between spraying and root watering; in summer, spray in the morning and evening to maintain humidity at 75%. Thinning: When the seedlings are 8cm tall, thin them to a spacing of 12cm between plants, and apply 600kg / mu of sheep manure as top dressing; Pest control: For snails, apply 450g / acre of metaldehyde; for spider mites, spray with a 1000-fold dilution of 15% pyridaben.

[0079] Example 4: Semi-wild cultivation method of Sagittaria sagittifolia (1) Deep plowing: Select steep slopes (35°-45°), manually plow to 30cm, and then expose the soil to the sun for 7 days. Apply 100kg / mu of lime for disinfection.

[0080] (2) Ridging of whole boxes: ridge width 1.5m (including ditch), ridge height 25cm, ditch width between ridges 50cm, ridge body in the form of steps, drainage ditch depth 35cm and ditch wall compacted.

[0081] (3) Wood block spreading: Three layers of water-hemp wood blocks (7cm long × 5cm wide × 3cm thick) are laid, with vertical and three-dimensional interlacing between the layers, and a total thickness of 8cm, forming a three-dimensional rotten wood layer on the ridge surface.

[0082] (4) Seed sowing: After the seeds are air-dried for 10 days, they are mixed with sawdust at a mass ratio of 1:50 and then mixed with 1% humic acid solution. Sow 1.5 pods per square meter and then lightly cover with 1cm of soil.

[0083] (5) Spreading decomposed leaves: Mix decomposed bamboo leaves with pine needles and cover with a 12cm thick layer. Press the edges with stones to prevent them from being blown away by the wind.

[0084] (6) Field management: Water: Combine drip irrigation and spraying, maintain humidity at 85% during the bulb enlargement period, and clear ditches before the rainy season; Thinning: When the seedlings are 10cm tall, thin them to a spacing of 15cm between plants, and apply 800kg / mu of sheep manure as top dressing; Pest control: Apply 3 kg / mu of phoxim before planting, and use 10% imidacloprid at a dilution of 1500 times during the growing season to control aphids.

[0085] The table below compares the germination rates of *Sagittaria sagittifolia* seeds cultivated under the cultivation conditions described in Examples 2-4: This invention utilizes a three-pronged approach of "number of wood block layers + thinning density + humidity control" to maintain a stable seedling density of 28,000-30,000 plants per acre, eliminating the need for accompanying fungal substrate bags and reducing costs by approximately 800-1000 yuan per acre. For steep slopes (35°-45°), the stepped ridge + 3-layer wood block scheme of Example 4 is preferred to ensure drainage and fungal colonization.

[0086] For gentle slopes (usually <15°), the two-layer wood blocks + dried bamboo leaves method of Example 3 can be used to fill the gaps, balancing cost and number of seedlings.

[0087] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

[0088] In summary, this invention provides a method for the semi-wild cultivation of *Cistanche deserticola*. Specific steps include: selecting shady mountain land at 30-45 degrees Celsius, deep plowing to a depth of 20-30 cm, sun-drying the soil, and then ridging the land; applying 50-100 kg of quicklime per mu for disinfection; processing fresh water-soluble wood into 5-8 cm × 3-5 cm × 2-3 cm blocks, drying them, and then layering them 2-3 times on the ridge surface. This three-dimensional structure naturally induces the colonization of symbiotic fungi, replacing the use of traditional companion fungal bags; in autumn, after 7-10 days of shade, mixing the seeds with sawdust at a 1:50 ratio and sowing them, covering them with 3-12 cm of well-rotted bamboo leaves to retain warmth and moisture, suppress weeds, and release organic matter. Field management includes misting for humidity control, temperature control at 15-25℃, thinning and fertilization, and pest and disease control. The semi-wild cultivation method for *Cistanche deserticola* provided by this invention achieves efficient cultivation through the construction of a biomimetic microenvironment and eliminates the need for additional companion fungal bags, significantly reducing cultivation costs. This invention utilizes the natural woody structure of water hemp wood blocks to provide a host environment for symbiotic fungi, eliminating the need for purchasing and cultivating associated fungal bags. Compared with existing technologies (such as CN117652342A, which requires spreading associated fungal granules), it reduces labor and material input. Combined with ridge-making on slopes and leaf littering, it constructs a micro-ecology that closely resembles the wild environment, which not only improves seedling survival rate and yield per acre, but also ensures that the effective components of medicinal materials meet the standards, thus achieving low-cost, sustainable large-scale cultivation.

Claims

1. A method for cultivating Morchella imitating wild growth, characterized in that, The method for cultivating the Morchella esculenta comprises the following steps: (1) Wood block treatment and laying: laying water-ma wood blocks on the ridge surface; (2) Seed treatment and sowing: after mixing the seeds with wet water-ma wood chips, sowing is performed; (3) Rotting leaf laying: laying rotting bamboo leaves after sowing; (4) Field management.

2. The Morchella wild imitation cultivation method according to claim 1, characterized in that, The cultivation mode of the Morchella esculenta is pot cultivation, preferably 30cm diameter flowerpot cultivation.

3. The Morchella wild imitation cultivation method according to claim 1, characterized in that, The cultivation mode of the Morchella esculenta is land cultivation, and before the step (1), a land arrangement step is further included, and the specific operation is as follows: after removing weeds and stones from the land, deep ploughing is performed, ridges are formed, and the soil is disinfected.

4. The Morchella wild imitation cultivation method according to claim 3, characterized in that, The specific operation of deep ploughing after removing weeds and stones from the land is as follows: a mountain land with a slope of 30-45 degrees and a pH value of 5.5-6.5 is selected, weeds and stones are removed, the soil is subjected to 20-30cm deep ploughing treatment, the soil is sunned for 5-7 days, base fertilizer is applied, and a drainage ditch is formed; The specific operation of forming ridges is as follows: ridges are formed according to the terrain, the ridge width is controlled to be 1.2-1.5m, the ridge height is set to be 20-25cm, the inter-ridge ditch width is 40-50cm, and the inter-ridge ditch depth is 30-35cm; The specific operation of soil disinfection is as follows: 50-100kg of lime is applied per mu of land, the soil is first ploughed and raked, the lime is uniformly applied after the soil is ploughed again, the lime is fully mixed with the soil, and the lime is not mixed with acid fertilizers or pesticides during use.

5. The Morchella wild imitation cultivation method according to any one of claims 1-4, characterized in that, The specific operation of wood block treatment and laying is as follows: fresh water-ma wood is selected, and wood blocks with a length of 5-8cm, a width of 3-5cm, and a thickness of 2-3cm are processed; the processed wood blocks are layered and laid on the ridge surface in two to three layers, and the wood blocks are arranged in a staggered manner, and the wood blocks are arranged in a vertical direction between layers, and the total thickness of the laid wood blocks is 3-10cm.

6. The Morchella wild imitation cultivation method according to claim 5, characterized in that, The total thickness of the laid wood blocks is 5-10cm.

7. The Morchella wild imitation cultivation method according to any one of claims 1-4, characterized in that, The specific operation of seed treatment and sowing is as follows: mature and full Morchella esculenta seeds free of diseases and pests are selected, and the seeds are dried in the shade, the thickness of the dried seeds does not exceed 2cm, and the drying time is 7-10 days; the treated seeds and wet water-ma wood chips are fully mixed according to a mass ratio of 1:30-70, and dry fruit hulls are uniformly sown on the surface of the ridge surface at a density of 1-1.5 per square meter.

8. The Morchella wild imitation cultivation method according to claim 7, characterized in that, The mass ratio of the seeds and wet water-ma wood chips is 1:50, and the density of the dry fruit hulls is 1.5 per square meter.

9. The Morchella wild imitation cultivation method according to any one of claims 1-4, characterized in that, The specific operation of rotting leaf laying is as follows: 3-12cm thick rotting bamboo leaves are laid on the surface layer of the ridge surface after sowing.

10. The Morchella wild imitation cultivation method according to any one of claims 1-4, characterized in that, The specific operation of field management includes: water management, temperature and light control, weed removal, inter-planting and fixing seedlings, additional fertilizer, and disease and pest control; In the water management, the humidity is maintained at 70%-85%; the temperature is maintained at 15-25℃; and the plant spacing of inter-planting is 10-15cm; The fertilizer is organic fertilizer, preferably sheep manure; the density of the additional fertilizer is 600kg / mu-800kg / mu; The medicine for disease and pest control is selected from tetraacetal, pyridaben, phoxim, or imidacloprid.

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