Radioactive skin injury model and preparation method thereof

Abstract

The invention relates to the technical field of biomedicine, in particular to a radioactive skin injury model and a preparation method thereof, and the preparation method comprises the following steps: preparing corium layer bio-ink by taking fibroblasts, a biodegradable high polymer material, a photoinitiator and a light absorbent as raw materials; preparing a skin corium layer model containing a blood vessel channel from the corium layer bio-ink; mixing the vascular endothelial cells with a gelatin solution, and cooling at 4 DEG C to obtain gelatin bio-ink; injecting gelatin bio-ink into a blood vessel channel of the skin corium layer model containing the blood vessel channel to obtain a skin model containing a blood vessel structure; the keratinocytes are planted on the surface of the skin model containing the vascular structure and then cultured, and the radioactive skin injury model is obtained. Bionic reconstruction of skin and vascular structures is achieved through the 3D printing technology, and the prepared radioactive skin injury model provides an ideal platform for screening of radioactive injury protection drugs and research of a repair mechanism.

Description

Technical Field

[0001] This invention relates to the field of biomedical technology, and in particular to a radiation-induced skin injury model and its preparation method. Background Technology

[0002] Radiation-induced skin injury is a common complication of radiotherapy and nuclear radiation exposure. Its pathogenesis is complex, mainly manifested as disruption of the skin barrier function, inflammatory response, vascular damage, cell apoptosis, and impaired repair. Clinically, radiation-induced skin injury can lead to erythema, edema, ulceration, necrosis, and even chronic, difficult-to-heal wounds, not only affecting patients' quality of life but also limiting the dosage and efficacy of radiotherapy. To further investigate the pathological mechanisms and dynamic processes of radiation-induced skin injury and to screen effective preventative and therapeutic drugs, it is crucial to establish an experimental model that highly simulates the actual damage process in the human body.

[0003] Currently, the main models used to study radiation-induced skin damage are animal models and in vitro skin models. Traditional animal models raise ethical concerns and are difficult to accurately reflect the differences in structure and response of human skin. To overcome the shortcomings of animal models, researchers have developed in vitro skin models. Currently, the main model used for skin damage is the two-dimensional cell culture model. This cell culture model usually uses a single type of skin cell (such as keratinocytes or fibroblasts) to be cultured in a monolayer in a culture dish and then irradiated.

[0004] Although this two-dimensional cell culture model reduces reliance on animals, it is difficult to accurately simulate the dynamic pathological process of radiation-induced skin damage, thus limiting its application. Summary of the Invention

[0005] To address the problem that existing skin models cannot accurately simulate the dynamic pathological process of radiation-induced skin damage, this invention provides a method for preparing a radiation-induced skin damage model. This method constructs biomimetic skin tissue containing vascular structures, enabling the prepared model to accurately simulate the occurrence and repair process of radiation-induced skin damage. This solves the problem that existing skin models cannot accurately simulate the dynamic pathological process of radiation-induced skin damage, providing an ideal platform for the screening of radiation-induced damage protective drugs and the study of repair mechanisms.

[0006] The technical solution adopted by this invention to solve its technical problem is: A method for preparing a radiation-induced skin injury model includes the following steps: S1: Cultivate fibroblasts and prepare dermal bio-ink using the fibroblasts, biodegradable polymer materials, photoinitiators, and light absorbers as raw materials; S2: The dermal bio-ink is used to prepare a skin dermal model containing vascular channels by 3D bioprinting; S3: Culture vascular endothelial cells, mix the vascular endothelial cells with gelatin solution, and cool at 4°C to obtain gelatin bio-ink; S4: Inject the gelatin bio-ink into the vascular channels of the dermal skin model containing vascular channels to obtain a skin model containing vascular structures; S5: Place the skin model containing the vascular structure in a 37°C, 5% CO2 incubator and add culture medium for incubation; S6: Cultivate keratinocytes and then seed the keratinocytes onto the surface of the skin model containing vascular structures and culture them to obtain a radiation-induced skin injury model.

[0007] Optionally, the diameter of the vascular channel ranges from 0.25 to 2 mm.

[0008] Optionally, the biodegradable polymer material is selected from at least one of the following: methacrylic acid modified collagen, methacrylic acid modified hyaluronic acid, methacrylic acid modified gelatin, methacrylic acid modified dextran, methacrylic acid derivative modified collagen, methacrylic acid derivative modified hyaluronic acid, methacrylic acid derivative modified gelatin, and methacrylic acid derivative modified dextran.

[0009] Optionally, the photoinitiator is lithium phenyl (2,4,6-trimethylbenzoyl)phosphinic acid.

[0010] Optionally, the volume ratio of the photoinitiator to the biodegradable polymer material is (1-2):(1-20).

[0011] Optionally, the light absorber is sodium 2,2'-dihydroxy-4,4'-dimethoxybenzophenone-5,5'-disulfonic acid.

[0012] Optionally, the volume ratio of the light absorber to the biodegradable polymer material is (1-2):(1-400).

[0013] Optionally, the concentration of the fibroblasts in the dermal bio-ink ranges from 2 × 10⁻⁶. 4 ~5×10 6 cells / mL.

[0014] Optionally, the concentration of the vascular endothelial cells in the gelatin bio-ink is in the range of 1×10⁻⁶. 4 ~8×10 6 cells / mL.

[0015] Another object of the present invention is to provide a radiation-induced skin injury model, which is prepared by the method described above for preparing a radiation-induced skin injury model.

[0016] The beneficial effects of this invention are: The method for preparing a radiation-induced skin injury model provided by this invention achieves biomimetic reconstruction of skin and vascular structures through 3D printing technology. The prepared radiation-induced skin injury model has a stable structure, high reproducibility, and combines structural biomimicry with functional controllability. It is suitable for research on radiation injury mechanisms and in vitro screening experiments of protective drugs and repair materials, providing an ideal platform for the screening of radiation injury protective drugs and the study of repair mechanisms. Attached Figure Description

[0017] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0018] Figure 1 This is a schematic diagram of the process for preparing the radiation-induced skin injury model in this invention; Figure 2 This is a three-dimensional model diagram of the radiation-induced skin injury model provided by the present invention; Figure 3 This is a staining result showing the cell viability of fibroblasts in the dermis of a radiation-induced skin injury model provided by this invention. Detailed Implementation

[0019] The present invention will now be described in further detail. The embodiments described below are exemplary and intended to explain the present invention, and should not be construed as limiting the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0020] To address the problem that existing skin models cannot accurately simulate the dynamic pathological process of radiation-induced skin injury, this invention provides a method for preparing a radiation-induced skin injury model. (See attached image.) Figure 1 As shown, the preparation method includes the following steps: S1: Culture fibroblasts and prepare dermal bio-ink using fibroblasts, biodegradable polymer materials, photoinitiators, and light absorbers as raw materials; Fibroblasts can be cultured according to the following process: Fibroblasts were revived and seeded in DMEM medium, and cultured at 37°C with 5% CO2, with the medium changed every 2 days. After the cells reached 80% confluence, they were passaged, centrifuged at 1000 rpm for 3 minutes, the supernatant was discarded, and the cell suspension was collected for later use.

[0021] The preparation process of dermal bio-ink is as follows: After digesting and centrifuging fibroblasts, a 1ml cell suspension was prepared using DMEM. After cell counting, the corresponding cell volume was centrifuged and mixed with biodegradable polymer materials. Photoinitiators and light absorbers were added to obtain dermal bio-ink.

[0022] S2: Use 3D bioprinting to prepare a skin dermal model containing vascular channels using dermal bio-ink; This step is performed as follows: After sterilizing the 3D bioprinter with ultraviolet light, dermal bio-ink is injected into a sterile ink cartridge, based on a pre-designed three-dimensional model containing vascular structures (see...). Figure 2 The process involves printing (as shown). During printing, the dermal bio-ink cross-links and solidifies under ultraviolet light, forming a biomimetic scaffold with a porous structure. Cells remain active and are evenly distributed throughout the material during printing, ultimately resulting in a dermal skin model containing vascular channels.

[0023] The preferred 3D modeling software for this invention is SOLIDWORKS and ZBRUSH.

[0024] S3: Culture vascular endothelial cells, mix the vascular endothelial cells with gelatin solution, and cool at 4°C to obtain gelatin bio-ink; Vascular endothelial cells can be cultured according to the following procedure: Resuscitate vascular endothelial cells and seed them in DMEM medium. Culture them at 37°C and 5% CO2, changing the medium every 2 days. Once the cells reach 80% confluence, passage them by centrifugation at 1000 rpm for 3 minutes, discarding the supernatant and collecting the cell suspension for later use.

[0025] Gelatin bio-ink is prepared according to the following process: After counting and centrifuging the vascular endothelial cells, the mixture was thoroughly mixed with a gelatin solution and cooled at 4°C for 5 minutes to obtain gelatin bio-ink.

[0026] S4: Inject gelatin bio-ink into the vascular channels of the dermal skin model containing vascular channels, and use the thermal reverse reaction properties of gelatin to make cells attach and form the inner wall of blood vessels, thus obtaining a skin model with vascular structures. S5: Place the skin model containing vascular structures in a 37°C, 5% CO2 incubator, add culture medium for culture, so that the cells are evenly distributed in the porous structure and maintain their activity. This step is performed as follows: The printed skin model containing vascular structures was placed in a 37°C, 5% CO2 incubator and cultured in DMEM medium, with the medium being changed every 2 days.

[0027] S6: Cultivate keratinocytes and then seed them onto the surface of a skin model for further cultivation to obtain a radiation-induced skin injury model; The culturing of keratinocytes is carried out according to the following procedure: Resuscitate keratinocytes and seed them in DMEM medium. Culture them at 37°C and 5% CO2, changing the medium every 2 days. Once the cells reach 80% confluence, passage them by centrifugation at 1000 rpm for 3 minutes. Discard the supernatant and collect the cell suspension for later use.

[0028] The implantation of keratinocytes is carried out according to the following procedure: After counting the keratinocytes, the corresponding cell volume was centrifuged, mixed with DMEM medium, and directly seeded into the surface grooves of the skin model. The cells were then placed in a 37°C, 5% CO2 incubator for culture to obtain a radiation-induced skin injury model.

[0029] The preferred concentration of keratinocytes is 1×10⁻⁶. 4 ~5×10 6 Approximately 100 cells / mL are needed to form complete skin tissue.

[0030] The method for preparing a radiation-induced skin injury model provided by this invention achieves biomimetic reconstruction of skin and vascular structures through 3D printing technology. The prepared radiation-induced skin injury model has a stable structure, high reproducibility, and combines structural biomimicry with functional controllability. It is suitable for research on radiation injury mechanisms and in vitro screening experiments of protective drugs and repair materials, providing an ideal platform for the screening of radiation injury protective drugs and the study of repair mechanisms.

[0031] The thickness of the radiation-induced skin injury model is preferably 2.5 mm. The vascular structure in this invention is established based on the size and distribution of blood vessels in the human dermis and generated using three-dimensional modeling software. The dermis contains vascular structures, and the diameter of the internal vascular channels is preferably in the range of 0.25 to 2 mm. This ensures the permeability and mechanical stability of the biomimetic blood vessels while guaranteeing the nutrient supply to cells and the excretion of metabolic products, thus providing a stable tissue basis for subsequent radiation treatment.

[0032] To better mimic skin tissue, the dermal bio-ink of this invention comprises biodegradable polymer materials and fibroblasts. Preferably, the biodegradable polymer materials are selected from at least one of the following: methacrylic acid-modified collagen, methacrylic acid-modified hyaluronic acid, methacrylic acid-modified gelatin, methacrylic acid-modified dextran, methacrylic acid derivative-modified collagen, methacrylic acid derivative-modified hyaluronic acid, methacrylic acid derivative-modified gelatin, and methacrylic acid derivative-modified dextran. Preferably, the methacrylic acid derivative-modified collagen is selected from at least one of methacrylic acid derivative-modified type I collagen and methacrylic acid derivative-modified type II collagen.

[0033] Specifically, the present invention preferably uses methacrylamide gelatin as the biodegradable polymer material to simulate the mechanical properties and extracellular matrix characteristics of skin.

[0034] This invention introduces biodegradable polymer materials to form a scaffold with a three-dimensional porous network structure, which is beneficial for observing cell adhesion, growth, and the repair process after radiation damage.

[0035] The preferred photoinitiator of this invention is lithium phenyl (2,4,6-trimethylbenzoyl)phosphinic acid, and the volume ratio of the photoinitiator to the biodegradable polymer material is further preferred to be (1-2):(1-20).

[0036] The preferred light absorber of the present invention is sodium 2,2'-dihydroxy-4,4'-dimethoxybenzophenone-5,5'-disulfonic acid, and the volume ratio of the light absorber to the biodegradable polymer material is further preferred to be (1-2):(1-400).

[0037] In step S2, during the preparation of the dermal skin model containing vascular channels, a fibroblast suspension was directly added to the bio-ink used for printing the dermis. The concentration of fibroblasts in the dermal bio-ink ranged from 2 × 10⁻⁶. 4 ~5×10 6 cells / mL.

[0038] The bio-ink for printing the dermis contains a suspension of fibroblasts. Therefore, the UV crosslinking time during the printing process should be appropriately reduced to 5-20 seconds. Before and after printing, in order to ensure the survival of cells in the hydrogel, the experimental operation time should be shortened as much as possible to achieve large-scale production in small batches.

[0039] The preferred concentration range of vascular endothelial cells in gelatin bio-ink according to this invention is 1×10⁻⁶. 4~8×10 6 cells / mL.

[0040] Furthermore, the method for preparing the radiation-induced skin injury model provided by the present invention may also include the step of constructing the radiation-induced skin injury model by irradiation.

[0041] Specifically, in step S6, the model is cultured at 37°C and 5% CO2, with the culture medium changed every two days. After the cells reach stable growth, they are irradiated to induce radiation damage in skin cells and vascular endothelial cells, thereby constructing a radiation-induced skin injury model. This model can realistically simulate cell apoptosis, inflammatory response, and vascular repair processes in radiation-induced skin injury, exhibiting good reproducibility and controllability. The preferred radiation dose range is 10–50 Gy, used to induce different degrees of radiation damage to skin cells and tissues to simulate radiation-induced inflammation, necrosis, and repair responses in the skin. The vascular pathways constructed in the radiation-induced skin injury model provided by this invention can be used to observe vascular damage, changes in permeability, and the repair process after irradiation. This radiation-induced skin injury model can be used for mechanistic studies of radiation-induced skin injury, screening of radioprotective drugs, and in vitro evaluation of wound repair materials.

[0042] This invention uses 3D printing technology to prepare the dermal structure of skin, introduces vascular pathways into the dermis, and lays a gelatin solution containing vascular endothelial cells into the vascular channels through the principle of thermal reverse reaction of gelatin, so that the endothelial cells are evenly distributed on the surface of the vascular channels. Subsequently, keratinocytes are implanted on the surface of the model to construct a complete skin structure, and radiation damage is induced in the skin tissue and cells by radiation irradiation, thereby obtaining a three-dimensional model that can simulate radiation-induced skin damage.

[0043] In summary, this invention provides a method for preparing a three-dimensional radiation-induced skin injury model. First, a three-dimensional skin structure model is constructed through image digitization, and then 3D photopolymerization printing is performed using cell-containing bio-ink (composed of hydrogel, collagen, photoinitiator, light absorber, and cell suspension). A vascular network is incorporated into the biomimetic skin tissue structure, and the thermal reverse reaction principle of gelatin is used to implant vascular endothelial cells, thereby forming physiologically functional vascular channels. Subsequently, keratinocytes are implanted to reconstruct complete skin tissue. Then, the three-dimensional skin model is subjected to radiation irradiation to induce radiation damage responses in the skin tissue layers and cells, simulating the pathological process of real radiation-induced skin injury. This model can be used to study cell apoptosis, inflammatory responses, vascular damage, and repair mechanisms in radiation-induced skin injury, providing an ideal in vitro experimental platform for screening drugs for radiation protection and treatment.

[0044] Another object of the present invention is to provide a radiation-induced skin injury model, which is prepared by the method described above for preparing a radiation-induced skin injury model.

[0045] The radiation-induced skin injury model provided by this invention achieves biomimetic reconstruction of skin and blood vessel structures through 3D printing technology. The prepared radiation-induced skin injury model has a stable structure and high reproducibility, and combines structural biomimicry with functional controllability. It is suitable for research on radiation injury mechanisms and in vitro screening experiments of protective drugs and repair materials, providing an ideal platform for the screening of radiation injury protective drugs and the study of repair mechanisms.

[0046] The radiation-induced skin injury model provided by this invention can not only simulate the necrosis, inflammation, and repair process of skin cells after radiation injury, but also realistically reflect pathological features such as changes in vascular permeability and nutritional metabolic disorders. In particular, radiation irradiation on a three-dimensional skin model containing vascular structures can establish a controllable and highly repeatable radiation-induced skin injury model, providing an ideal in vitro experimental platform for elucidating its pathogenesis, screening protective drugs, and developing repair materials.

[0047] The three-dimensional radiation-induced skin injury model provided by this invention has a complete structure, biomimetic function, and controllable response. It can not only systematically study the occurrence and repair mechanism of radiation injury, but also be used for the screening and evaluation of radiation protection drugs and wound repair materials.

[0048] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0049] Unless otherwise specified, all reagents and materials used in the embodiments of this invention can be purchased from the market.

[0050] Example 1 This embodiment provides a method for preparing a radiation-induced skin injury model, including the following steps: 1. Preparation of a three-dimensional skin model containing vascular structures The basic skin construction process of the three-dimensional radiation-induced skin damage model is as follows: Figure 1 As shown, the specific steps are as follows: 1) Culture of fibroblasts: Fibroblasts were revived and seeded in DMEM medium, and cultured at 37°C with 5% CO2, with the medium changed every 2 days. After the cells reached 80% confluence, they were passaged, centrifuged at 1000 rpm for 3 minutes, the supernatant was discarded, and the cell suspension was collected for later use.

[0051] 2) Preparation of dermal bio-ink: After digesting and centrifuging fibroblasts, a 1ml cell suspension was prepared using DMEM. After cell counting, the corresponding cell volume was centrifuged and mixed with biodegradable polymer materials. Photoinitiators and light absorbers were added to obtain dermal bio-ink.

[0052] The biodegradable polymer material was methacrylated gelatin, the photoinitiator was lithium phenyl(2,4,6-trimethylbenzoyl)phosphine, and the light absorber was sodium 2,2'-dihydroxy-4,4'-dimethoxybenzophenone-5,5'-disulfonate. The volume ratio of the photoinitiator to the biodegradable polymer material was 1:15, and the volume ratio of the light absorber to the biodegradable polymer material was 1:200. The concentration of fibroblasts in the dermal bio-ink was 5 × 10⁻⁶. 5 cells / mL; 3) Printing of the dermal layer model of the skin: After the 3D bioprinter is sterilized with ultraviolet light, bio-ink is injected into a sterile ink cartridge and printed according to a pre-designed three-dimensional model containing a vascular structure. The ultraviolet cross-linking time during the printing process is 15 seconds. The bio-ink cross-links and solidifies under ultraviolet light to form a biomimetic scaffold with a porous structure. Cells remain active and are evenly distributed in the material during the printing process.

[0053] 2. Introduction and culture of vascular endothelial cells 1) Culture of vascular endothelial cells: Resuscitate vascular endothelial cells and seed them in DMEM medium. Culture them at 37°C and 5% CO2, changing the medium every 2 days. Once the cells reach 80% confluence, passage them by centrifugation at 1000 rpm for 3 minutes, discarding the supernatant and collecting the cell suspension for later use.

[0054] 2) Formulation of gelatin bio-ink: After counting and centrifuging the vascular endothelial cells, they were mixed evenly with a gelatin solution and cooled at 4°C for 5 minutes. The resulting ink was then injected into the printed vascular channel, utilizing the thermal reverse reaction properties of gelatin to allow the cells to attach and form the inner wall of the blood vessel.

[0055] The concentration of vascular endothelial cells in gelatin bio-ink was 8 × 10⁻⁶. 5 cells / mL.

[0056] 3) Cultivation conditions: The printed skin model was placed in a 37°C, 5% CO2 incubator and cultured in DMEM medium, which was changed every two days. The model was 2.5 mm thick with an internal vascular pathway of 1 mm, ensuring adequate nutrient supply and metabolic waste removal for the cells, thus providing a stable tissue basis for subsequent radiation treatment.

[0057] 3. Keratinocyte implantation 1) Culture of keratinocytes: Resuscitate keratinocytes and seed them in DMEM medium. Culture them at 37°C and 5% CO2, changing the medium every 2 days. Once the cells reach 80% confluence, passage them by centrifugation at 1000 rpm for 3 minutes. Discard the supernatant and collect the cell suspension for later use.

[0058] 2) Cultivation of keratinocytes: After counting the keratinocytes, the corresponding cell volume was centrifuged, mixed with DMEM medium, and directly seeded into the surface grooves of the 3D model. Then, it was placed in a 37°C, 5% CO2 incubator for culture.

[0059] The concentration of keratinocytes is 5×10 5 cells / mL.

[0060] 4. Constructing a radiation-induced skin injury model through radiation irradiation Once the skin model has stabilized in culture and the cells are growing well, it is placed in an X-ray irradiation device for radiation treatment. The irradiation dose is controlled between 10 and 50 Gy (set according to experimental requirements) to induce different degrees of radiation damage. After irradiation, the model is cultured again to observe cell activity, inflammatory response, changes in vascular permeability, and the repair process.

[0061] After radiation exposure, the model exhibited typical characteristics of radiation-induced skin damage: increased apoptosis, decreased activity of fibroblasts in the dermis, partial shedding and structural collapse of vascular endothelial cells, and the overall color of the model changed from light to dark, with mild cracks appearing inside the tissue, simulating the early pathological changes of real radiation-induced skin damage.

[0062] 5. Cell viability detection and morphological observation Cells in the model before and after radiation irradiation were stained and analyzed using live / dead staining reagents, such as... Figure 3 As shown in the figure. The results indicate that before irradiation, the cells in the model were uniformly distributed with a survival rate of approximately 90%; after irradiation, the cell survival rate decreased to approximately 60%, and the cells morphologically shrank and became disordered. This demonstrates that the model can effectively reflect the cellular changes of radiation-induced skin injury and has good bioreproducibility.

[0063] In summary, the three-dimensional radiation-induced skin injury model provided by this invention achieves biomimetic reconstruction of skin and vascular structures through 3D printing technology, and, combined with X-ray irradiation-induced damage, successfully simulates the main pathological features of radiation-induced skin injury. This model is structurally stable and highly reproducible, making it suitable for research on radiation damage mechanisms and in vitro screening experiments for protective drugs and repair materials.

[0064] Based on the above-described preferred embodiments of the present invention, and through the foregoing description, those skilled in the art can make various changes and modifications without departing from the inventive concept. The technical scope of this invention is not limited to the contents of the specification, but must be determined according to the scope of the claims.

Claims

1. A method for preparing a radiation-induced skin injury model, characterized in that, Includes the following steps: S1: Cultivate fibroblasts and prepare dermal bio-ink using the fibroblasts, biodegradable polymer materials, photoinitiators, and light absorbers as raw materials; S2: The dermal bio-ink is used to prepare a skin dermal layer model containing vascular channels by 3D bioprinting; S3: Culture vascular endothelial cells, mix the vascular endothelial cells with gelatin solution, and cool at 4°C to obtain gelatin bio-ink; S4: Inject the gelatin bio-ink into the vascular channels of the dermal skin model containing vascular channels to obtain a skin model containing vascular structures; S5: Place the skin model containing the vascular structure in a 37°C, 5% CO2 incubator and add culture medium for incubation; S6: Cultivate keratinocytes and then seed the keratinocytes onto the surface of the skin model containing vascular structures and culture them to obtain a radiation-induced skin injury model.

2. The method for preparing a radiation-induced skin injury model as described in claim 1, characterized in that, The diameter of the vascular channel ranges from 0.25 to 2 mm.

3. The method for preparing a radiation-induced skin injury model as described in claim 1, characterized in that, The biodegradable polymer material is selected from at least one of the following: methacrylic acid modified collagen, methacrylic acid modified hyaluronic acid, methacrylic acid modified gelatin, methacrylic acid modified dextran, methacrylic acid derivative modified collagen, methacrylic acid derivative modified hyaluronic acid, methacrylic acid derivative modified gelatin, and methacrylic acid derivative modified dextran.

4. The method for preparing a radiation-induced skin injury model as described in claim 1, characterized in that, The photoinitiator is lithium phenyl (2,4,6-trimethylbenzoyl)phosphinic acid.

5. The method for preparing a radiation-induced skin injury model as described in claim 1, characterized in that, The volume ratio of the photoinitiator to the biodegradable polymer material is (1-2):(1-20).

6. The method for preparing a radiation-induced skin injury model as described in claim 1, characterized in that, The light absorber is sodium 2,2'-dihydroxy-4,4'-dimethoxybenzophenone-5,5'-disulfonic acid.

7. The method for preparing a radiation-induced skin injury model as described in claim 1, characterized in that, The volume ratio of the light absorber to the biodegradable polymer material is (1-2):(1-400).

8. The method for preparing a radiation-induced skin injury model as described in claim 1, characterized in that, The concentration of the fibroblasts in the dermal bio-ink is in the range of 2 × 10⁻⁶. 4 ~5×10 6 cells / mL.

9. The method for preparing a radiation-induced skin injury model as described in claim 1, characterized in that, The concentration range of the vascular endothelial cells in the gelatin bio-ink is 1×10⁻⁶. 4 ~8×10 6 cells / mL.

10. A radiation-induced skin injury model, characterized in that, The radiation-induced skin injury model is prepared using the method described in any one of claims 1-9.

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