Preparation and use method of chitosan oligosaccharide antifreeze agent

Abstract

The invention relates to the technical field of agricultural production, and particularly discloses a preparation and use method of a chitosan oligosaccharide antifreeze agent, the antifreeze agent comprises the following components by weight: 1-3 g / L of potassium dihydrogen phosphate, 0.01-0.03 mg / L of brassinolide, 0.5-1 g / L of amino acid, 25-50 mg / L of chitosan oligosaccharide powder (10%), 0.01-0.04 mg / L of glutamic acid, 0.01-0.04 mg / L of lysine, 0.2-0.4 mg / L of pyruvic acid, 0.1-0.2 mg / L of citric acid, 0.01-0.02 mg / L of glycerin, 20-50 g / L of an emulsifier, and the balance of water. 10-20 g / L of SiO2 nano-particles and a trace element fertilizer; according to the invention, through cooperation of the elements in the agent, on the basis of supplementing nutrient elements of plants, the lignification degree of the plants is enhanced, the freezing resistance of the plants is enhanced, the plants are induced to generate stress resistance, the SiO2 nanoparticles are added, so that the anti-freezing agent is adsorbed in the nanoparticles, slow release of the drug effect of the anti-freezing agent can be realized, and the anti-freezing effect of the plants is improved. The pesticide effect timeliness after single-time spraying of the anti-freezing agent is prolonged.

Description

Technical Field

[0001] This invention belongs to the field of agricultural production technology, specifically relating to the preparation and application method of a chitosan oligosaccharide antifreeze agent. Background Technology

[0002] Chitosan oligosaccharide, also known as chitosan oligosaccharide or chitosan oligosaccharide, has been the subject of extensive research in recent years. Research has shown that chitosan oligosaccharide can effectively increase the yield of fruits and vegetables, prevent and control pests and diseases, and enhance the resistance of fruits and vegetables. As a result, chitosan oligosaccharide antifreeze agents have emerged.

[0003] However, the existing chitosan oligosaccharide antifreeze agents have a relatively complicated preparation process and a short effective time, making them unsuitable for large-scale promotion and inconsistent with the significance of increasing production and sustainable development in the planting industry.

[0004] Therefore, there is an urgent need for a chitosan oligosaccharide antifreeze agent and its application method. Summary of the Invention

[0005] The purpose of this invention is to provide a method for preparing and using chitosan oligosaccharide antifreeze, in order to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] A chitosan oligosaccharide antifreeze agent, comprising: the antifreeze agent being composed of the following components in parts by weight: potassium dihydrogen phosphate 1-3 g / L, brassinolide 0.01-0.03 mg / L, amino acids 0.5-1 g / L, chitosan oligosaccharide powder (10%) 25-50 mg / L, glutamic acid 0.01-0.04 mg / L, lysine 0.01-0.04 mg / L, pyruvic acid 0.2-0.4 mg / L, citric acid 0.1-0.2 mg / L, glycerol 0.01-0.02 mg / L, emulsifier 20-50 g / L, SiO2 nanoparticles and trace element fertilizer 10-20 g / L.

[0008] Preferably, the micronutrient fertilizer includes one or more of boric acid, manganese carbonate, manganese nitride, integrated copper, and ferrous ammonium sulfate.

[0009] Preferably, the emulsifier includes one or more of white sugar, propylene glycol, sodium potassium tartrate, and diethylenetriaminepentaacetic acid.

[0010] A method for preparing a chitosan oligosaccharide antifreeze agent includes the following steps:

[0011] S1. According to the formula and in proportion to the amount of water per liter, add the following components to the reaction vessel: potassium dihydrogen phosphate, brassinolide, amino acids, chitosan oligosaccharide, glutamic acid, lysine, pyruvic acid, citric acid, and glycerol. Then add deionized water to dissolve them. Place the reaction vessel in a constant temperature water bath at 35-40℃. Stir the mixture inside the reaction vessel with a glass rod for 30-60 minutes to make the internal solution homogeneous and bring it to a fixed volume to obtain the antifreeze solution.

[0012] S2. Next, add the emulsifier to the reaction vessel in S1 and stir evenly with a stirring rod.

[0013] S3. Adding phosphoric acid will adjust the pH of the antifreeze solution to 5.0-6.5;

[0014] S4. Add trace element fertilizer and SiO2 nanoparticles to the antifreeze solution, and place the mixture of nanoparticles and drug solution in a constant temperature shaker for shaking to finally prepare a mixed solution.

[0015] S5. Observe the product's color and whether there are any impurities, and check the product's stability before packaging and storing it in the warehouse.

[0016] Preferably, in S1, the volume ratio of the composite chitosan oligosaccharide agent to deionized water is 2:3.

[0017] Preferably, in step S4, the internal temperature of the constant temperature oscillator is 20-25°C, the shaking time is 10-12 hours, and the ratio between the antifreeze solution, trace element fertilizer and SiO2 nanoparticles is 1:0.3:0.5.

[0018] A method for using a chitosan oligosaccharide antifreeze agent includes the following steps: dilute it to a 150-200 times solution and spray it by spraying the entire plant through the stems and leaves. Before a cold wave arrives or before or after flowering in early spring, spray once every 7 days, for a total of 2-3 sprays. If there is a sudden drop in temperature, spray 1-2 times before the temperature drops, preferably around 10-12 noon.

[0019] Compared with the prior art, the beneficial effects of the present invention are:

[0020] (1) Through the combination of various elements in the agent, potassium dihydrogen phosphate can quickly replenish nutrients, maintain the nutrient balance of the plant, and improve the lignification degree of the plant. Phosphorus increases the sugar and phospholipid levels, potassium can regulate stomata to inhibit evaporation and enhance the cold resistance of the tree. Amino acids can increase the content of unsaturated fatty acids in membrane lipids, prevent the phase change of biological membranes, stabilize membrane structure, improve photosynthesis, etc., thereby enhancing cold resistance. Brassinolide and chitosan oligosaccharide can induce plants to produce resistant enzymes to resist cold. This antifreeze solution, on the basis of replenishing plant nutrients, enhances the lignification degree of the plant, not only enhancing its own frost resistance, but also inducing the plant to produce stress resistance.

[0021] (2) By adding SiO2 nanoparticles, the antifreeze agent is adsorbed inside the nanoparticles, which enables the slow release of the antifreeze agent's efficacy, prolongs the duration of the antifreeze agent's efficacy after a single spray, and improves the antifreeze effect. Attached Figure Description

[0022] Figure 1 This is a flowchart illustrating the preparation process of the chitosan oligosaccharide antifreeze agent of the present invention. Detailed Implementation

[0023] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0024] Example 1:

[0025] Please see Figure 1 As shown, a chitosan oligosaccharide antifreeze agent comprises the following components by weight: potassium dihydrogen phosphate 2 g / L, brassinolide 0.02 mg / L, amino acids 0.75 mg / L, chitosan oligosaccharide powder (10%) 35 mg / L, glutamic acid 0.03 mg / L, lysine 0.03 mg / L, pyruvic acid 0.3 mg / L, citric acid 0.1 mg / L, glycerol 0.01 mg / L, emulsifier 30 g / L, SiO2 nanoparticles and trace element fertilizer 15 g / L.

[0026] The micronutrient fertilizer includes one or more of boric acid, manganese carbonate, manganese nitride, integrated copper, and ferrous ammonium sulfate.

[0027] The emulsifier includes one or more of white sugar, propylene glycol, sodium potassium tartrate, and diethylenetriaminepentaacetic acid.

[0028] A method for preparing a chitosan oligosaccharide antifreeze agent includes the following steps:

[0029] S1. According to the formula and in proportion to the amount of water per liter, add the following components to the reaction vessel: potassium dihydrogen phosphate, brassinolide, amino acids, chitosan oligosaccharide, glutamic acid, lysine, pyruvic acid, citric acid, and glycerol. Then add deionized water to dissolve them. Place the reaction vessel in a constant temperature water bath at 35-40℃. Stir the mixture inside the reaction vessel with a glass rod for 30-60 minutes to make the internal solution homogeneous and bring it to a fixed volume to obtain the antifreeze solution.

[0030] S2. Next, add the emulsifier to the reaction vessel in S1 and stir evenly with a stirring rod.

[0031] S3. Adding phosphoric acid will adjust the pH of the antifreeze solution to 5.0-6.5;

[0032] S4. Add trace element fertilizer and SiO2 nanoparticles to the antifreeze solution, and place the mixture of nanoparticles and drug solution in a constant temperature shaker for shaking to finally prepare a mixed solution.

[0033] S5. Observe the product's color and whether there are any impurities, and check the product's stability before packaging and storing it in the warehouse.

[0034] In S1, the volume ratio of the complex chitosan oligosaccharide agent to deionized water is 2:3.

[0035] In S4, the internal temperature of the constant temperature oscillator is 20-25℃, the shaking time is 10-12 hours, and the ratio between the antifreeze solution, trace element fertilizer and SiO2 nanoparticles is 1:0.5:0.5.

[0036] A method for using a chitosan oligosaccharide antifreeze agent includes the following steps: dilute it to a 150-200 times solution and spray it by spraying the entire plant through the stems and leaves. Before a cold wave arrives or before or after flowering in early spring, spray it once every 4 days for a total of 3 sprays. If there is a sudden drop in temperature, spray it twice before the temperature drops, preferably around 10-12 noon.

[0037] Example 2:

[0038] Please see Figure 1 As shown, a chitosan oligosaccharide antifreeze agent comprises the following components by weight: potassium dihydrogen phosphate 3g / L, brassinolide 0.03mg / L, amino acids 1g / L, chitosan oligosaccharide powder (10%) 50mg / L, glutamic acid 0.04mg / L, lysine 0.04mg / L, pyruvic acid 0.4mg / L, citric acid 0.2mg / L, glycerol 0.02mg / L, emulsifier 50g / L, SiO2 nanoparticles and trace element fertilizer 20g / L.

[0039] The micronutrient fertilizer includes one or more of boric acid, manganese carbonate, manganese nitride, integrated copper, and ferrous ammonium sulfate.

[0040] The emulsifier includes one or more of white sugar, propylene glycol, sodium potassium tartrate, and diethylenetriaminepentaacetic acid.

[0041] A method for preparing a chitosan oligosaccharide antifreeze agent includes the following steps:

[0042] S1. According to the formula and in proportion to the amount of water per liter, add the following components to the reaction vessel: potassium dihydrogen phosphate, brassinolide, amino acids, chitosan oligosaccharide, glutamic acid, lysine, pyruvic acid, citric acid, and glycerol. Then add deionized water to dissolve them. Place the reaction vessel in a constant temperature water bath at 35-40℃. Stir the mixture inside the reaction vessel with a glass rod for 30-60 minutes to make the internal solution homogeneous and bring it to a fixed volume to obtain the antifreeze solution.

[0043] S2. Next, add the emulsifier to the reaction vessel in S1 and stir evenly with a stirring rod.

[0044] S3. Adding phosphoric acid will adjust the pH of the antifreeze solution to 5.0-6.5;

[0045] S4. Add trace element fertilizer and SiO2 nanoparticles to the antifreeze solution, and place the mixture of nanoparticles and drug solution in a constant temperature shaker for shaking to finally prepare a mixed solution.

[0046] S5. Observe the product's color and whether there are any impurities, and check the product's stability before packaging and storing it in the warehouse.

[0047] In S1, the volume ratio of the complex chitosan oligosaccharide agent to deionized water is 2:3.

[0048] In S4, the internal temperature of the constant temperature oscillator is 20-25℃, the shaking time is 10-12 hours, and the ratio between the antifreeze solution, trace element fertilizer and SiO2 nanoparticles is 1:0.5:1.

[0049] A method for using a chitosan oligosaccharide antifreeze agent includes the following steps: dilute it to a 150-200 times solution and spray it by spraying the entire plant through the stems and leaves. Spray once every 7 days before a cold wave arrives or before or after flowering in early spring, for a total of 2 sprays; if there is a sudden drop in temperature, spray once before the temperature drops, preferably around 10-12 noon.

[0050] Example 3:

[0051] Please see Figure 1 As shown, a chitosan oligosaccharide antifreeze agent comprises the following components in parts by weight: potassium dihydrogen phosphate 1 g / L, brassinolide 0.013 mg / L, amino acids 0.5 g / L, chitosan oligosaccharide powder (10%) 25 mg / L, glutamic acid 0.01 mg / L, lysine 0.01 mg / L, pyruvic acid 0.2 mg / L, citric acid 0.1 mg / L, glycerol 0.01 mg / L, emulsifier 20 g / L, SiO2 nanoparticles and trace element fertilizer 10 g / L.

[0052] The micronutrient fertilizer includes one or more of boric acid, manganese carbonate, manganese nitride, integrated copper, and ferrous ammonium sulfate.

[0053] The emulsifier includes one or more of white sugar, propylene glycol, sodium potassium tartrate, and diethylenetriaminepentaacetic acid.

[0054] A method for preparing a chitosan oligosaccharide antifreeze agent includes the following steps:

[0055] S1. According to the formula and in proportion to the amount of water per liter, add the following components to the reaction vessel: potassium dihydrogen phosphate, brassinolide, amino acids, chitosan oligosaccharide, glutamic acid, lysine, pyruvic acid, citric acid, and glycerol. Then add deionized water to dissolve them. Place the reaction vessel in a constant temperature water bath at 35-40℃. Stir the mixture inside the reaction vessel with a glass rod for 30-60 minutes to make the internal solution homogeneous and bring it to a fixed volume to obtain the antifreeze solution.

[0056] S2. Next, add the emulsifier to the reaction vessel in S1 and stir evenly with a stirring rod.

[0057] S3. Adding phosphoric acid will adjust the pH of the antifreeze solution to 5.0-6.5;

[0058] S4. Add trace element fertilizer and SiO2 nanoparticles to the antifreeze solution, and place the mixture of nanoparticles and drug solution in a constant temperature shaker for shaking to finally prepare a mixed solution.

[0059] S5. Observe the product's color and whether there are any impurities, and check the product's stability before packaging and storing it in the warehouse.

[0060] In S1, the volume ratio of the complex chitosan oligosaccharide agent to deionized water is 2:3.

[0061] In S4, the internal temperature of the constant temperature oscillator is 20-25℃, the shaking time is 10-12 hours, and the ratio between the antifreeze solution, trace element fertilizer and SiO2 nanoparticles is 1:0.5:0.5.

[0062] A method for using a chitosan oligosaccharide antifreeze agent includes the following steps: dilute it to a 150-200 times solution and spray it by spraying the entire plant through the stems and leaves. Before a cold wave arrives or before or after flowering in early spring, spray it once every 3 days for a total of 3 sprays. If there is a sudden drop in temperature, spray it twice before the temperature drops, preferably around 10-12 noon.

[0063] In summary, by combining the various elements within the antifreeze agent, the liquid not only replenishes the plant's nutrients but also enhances the plant's lignification, thereby increasing its frost resistance and inducing stress resistance. Furthermore, the addition of nano-slow-release agents enables the slow release of the antifreeze agent's efficacy, extending the duration of its effect after a single spray.

[0064] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A chitosan oligosaccharide antifreeze agent, characterized in that, include: The antifreeze agent is composed of the following components by weight: potassium dihydrogen phosphate 1-3 g / L, brassinolide 0.01-0.03 mg / L, amino acids 0.5-1 g / L, chitosan oligosaccharide powder (10%) 25-50 mg / L, glutamic acid 0.01-0.04 mg / L, lysine 0.01-0.04 mg / L, pyruvic acid 0.2-0.4 mg / L, citric acid 0.1-0.2 mg / L, glycerol 0.01-0.02 mg / L, emulsifier 20-50 g / L, SiO2 nanoparticles and trace element fertilizer 10-20 g / L.

2. The chitosan oligosaccharide antifreeze agent according to claim 1, characterized in that: The micronutrient fertilizer includes one or more of boric acid, manganese carbonate, manganese nitride, integrated copper, and ferrous ammonium sulfate.

3. The chitosan oligosaccharide antifreeze agent according to claim 1, characterized in that: The emulsifier includes one or more of white sugar, propylene glycol, sodium potassium tartrate, and diethylenetriaminepentaacetic acid.

4. A method for preparing a chitosan oligosaccharide antifreeze agent, characterized in that, Includes the following steps: S1. According to the formula and in proportion to the amount of water per liter, add the following components to the reaction vessel: potassium dihydrogen phosphate, brassinolide, amino acids, chitosan oligosaccharide, glutamic acid, lysine, pyruvic acid, citric acid, and glycerol. Then add deionized water to dissolve them. Place the reaction vessel in a constant temperature water bath at 35-40℃. Stir the mixture inside the reaction vessel with a glass rod for 30-60 minutes to make the internal solution homogeneous and bring it to a fixed volume to obtain the antifreeze solution. S2. Next, add the emulsifier to the reaction vessel in S1 and stir evenly with a stirring rod. S3. Adding phosphoric acid will adjust the pH of the antifreeze solution to 5.0-6.5; S4. Add trace element fertilizer and SiO2 nanoparticles to the antifreeze solution, and place the mixture of nanoparticles and drug solution in a constant temperature shaker for shaking to finally prepare a mixed solution. S5. Observe the product's color and whether there are any impurities, and check the product's stability before packaging and storing it in the warehouse.

5. The method for preparing a chitosan oligosaccharide antifreeze agent according to claim 4, characterized in that: In S1, the volume ratio of the complex chitosan oligosaccharide agent to deionized water is 2:

3.

6. The method for preparing a chitosan oligosaccharide antifreeze agent according to claim 4, characterized in that: In S4, the internal temperature of the constant temperature oscillator is 20-25℃, the shaking time is 10-12 hours, and the ratio between the antifreeze solution, trace element fertilizer and SiO2 nanoparticles is 1:0.5:0.

5.

7. A method of using a chitosan oligosaccharide antifreeze agent, comprising the following steps: Dilute to 150-200 times and spray, spraying the entire plant by spraying the stems and leaves. Before a cold wave or before or after flowering in early spring, spray once every 7 days, for a total of 2-3 times. If there is a sudden drop in temperature, spray 1-2 times before the temperature drops, preferably between 10 am and 12 pm.

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