Veterinary taurine powder for regulating body immunity and preparation process thereof

Abstract

The invention belongs to the technical field of veterinary medicines, and particularly relates to veterinary taurine powder for regulating body immunity and a preparation process of the veterinary taurine powder. The veterinary taurine powder for regulating body immunity is prepared from taurine, chicoric acid and glycyrrhiza polysaccharide in a mass ratio of (3-4): (0.5-1): (1-1.5). Through scientific compounding, the taurine, the chicoric acid and the glycyrrhiza polysaccharide in the formula can form a more effective synergistic effect of regulating body immunity, so that the drug effect and the function are more comprehensive. The powder is simple in preparation method, low in cost, high in safety, very suitable for large-scale industrial production and use and wide in clinical prospect.

Description

Technical Field

[0001] This invention belongs to the field of veterinary medicine technology, specifically relating to a veterinary taurine powder for regulating the body's immunity and its preparation process. Background Technology

[0002] Taurine is a semi-essential amino acid and one of the most abundant free amino acids in animal tissues. By enhancing the precise positioning of codons and anticodons, taurine can more effectively maintain the integrity of the electron transport chain structure and function, and prevent electron leakage caused by mistranslation of electron transport chain-related proteins, thus reducing the generation of reactive oxygen species. Taurine is synthesized in the liver from methionine or cysteine, and plays a crucial role in maintaining cellular homeostasis and metabolic balance. Newborns and young children cannot synthesize taurine like adults, or some tissues require exogenous supplementation under specific physiological conditions to meet their growth, development, and physiological functions; therefore, taurine is also known as a conditionally essential amino acid.

[0003] In the field of animal husbandry, taurine has gained widespread attention as a functional feed additive. Studies have confirmed that it has antioxidant, anti-inflammatory, lipid metabolism homeostasis regulation, and intestinal microecological balance improvement effects, which can improve animal growth performance, feed efficiency, intestinal barrier function and product quality, while effectively alleviating various stress responses.

[0004] The immune system is a crucial site for the body's immune response and immune function. In veterinary clinical practice, when an animal's immune system is damaged, immunosuppressive diseases emerge, such as infectious bursal disease in chickens, avian leukosis, Marek's disease, and porcine reproductive and respiratory syndrome (PRRS). Immunosuppressive diseases are a general term for diseases in which the animal's immune system is severely damaged under the influence of single or multiple pathogenic factors, resulting in immune dysregulation, temporary or permanent impairment of the body's immune response, and increased susceptibility to various pathogens. The most prominent clinical manifestations of immunosuppressive diseases are increased susceptibility to pathogens, higher morbidity and mortality rates, and decreased immune cell activity.

[0005] With the gradual implementation of my country's comprehensive ban on antibiotics, the pig farming industry faces the dual pressures of sub-health issues and disease risks. The ban on antibiotics has led to frequent problems such as gut microbiota imbalance and immunosuppression in pigs. Therefore, improving the immunity of pig herds has become a key task in current farming practices. A healthy immune function is the core mechanism for pigs to resist disease and a crucial factor in ensuring the health of the pig herd.

[0006] Therefore, it is urgent to find a veterinary drug with high safety, clearly defined active ingredients, and better ability to regulate the body's immunity. Summary of the Invention

[0007] To overcome the shortcomings and deficiencies of the prior art, this invention provides a veterinary taurine powder for regulating the body's immunity and its application. It has a reasonable formulation, good effect on regulating the body's immunity, no toxic side effects, and can effectively solve the problems existing in the prior art.

[0008] This invention provides a veterinary taurine composition for regulating the body's immunity, wherein the veterinary taurine composition for regulating the body's immunity is composed of the following raw materials: taurine, chicoric acid and glycyrrhizin.

[0009] Furthermore, the veterinary taurine composition for regulating the body's immunity is composed of taurine, chicoric acid, and glycyrrhizic acid in a mass ratio of 3-4:0.5-1:1-1.5.

[0010] Furthermore, the veterinary taurine composition for regulating the body's immunity is composed of taurine, chicoric acid, and glycyrrhizic acid in a mass ratio of 3:0.5:1.

[0011] Furthermore, the veterinary taurine composition for regulating the body's immunity is composed of taurine, chicoric acid, and glycyrrhizic acid in a mass ratio of 4:0.5:1.5.

[0012] Furthermore, the veterinary taurine composition for regulating the body's immunity is composed of taurine, chicoric acid, and glycyrrhizic acid in a mass ratio of 3:1:1.

[0013] The preparation method of the veterinary taurine composition for regulating the body's immunity according to the present invention includes the following steps: weighing each raw material component according to any one of the formulation components described in claims 1-5; mixing the weighed components to obtain the veterinary taurine composition for regulating the body's immunity.

[0014] This invention provides a veterinary traditional Chinese medicine preparation comprising the above-mentioned veterinary taurine composition.

[0015] Furthermore, the dosage form of the traditional Chinese medicine preparation is powder, ointment, or spray.

[0016] Furthermore, the particle size of the powder is less than 160 mesh.

[0017] This invention provides the use of the veterinary taurine composition for regulating the body's immunity and the veterinary traditional Chinese medicine preparation in the preparation of veterinary drugs for regulating the body's immunity.

[0018] As can be seen from the above technical solution, compared with the prior art, the present invention provides a veterinary taurine composition or taurine powder for regulating the body's immunity and its preparation process, which has the following beneficial effects: Taurine, as a nutrient, participates in the body's growth and metabolism, and also plays a vital role in maintaining immune function. It has important regulatory functions in the nervous, digestive, reproductive, cardiovascular, immune, and endocrine systems. As a conditionally semi-essential amino acid in animals, taurine participates in maintaining immune function and enhancing both specific and non-specific immunity.

[0019] Chicoric acid, a derivative of caffeic acid, is one of the most important immunomodulatory components in Echinacea purpurea. Pharmacological studies have shown that chicoric acid enhances immune function, has anti-inflammatory effects, inhibits hyaluronidase, and protects collagen I from free radicals that can lead to degradation.

[0020] The bioactivity of glycyrrhiza polysaccharides affects intercellular signal transduction, playing a significant role in antioxidation, immune regulation, antitumor activity, anti-inflammation, and regulation of gut microbiota. As a natural plant extract, glycyrrhiza polysaccharides have made significant progress in the application of poultry and pig production, improving growth performance, enhancing immunity, and improving feed conversion rate.

[0021] Immunoglobulin levels are important indicators for assessing an animal's immune status and health. IgA, IgM, and IgG, as three core immunoglobulins, work synergistically to construct a multi-layered immune defense system. This invention's veterinary taurine powder significantly increases the levels of IgA, IgG, and IgM in serum, effectively enhancing the immunity of fattening cattle.

[0022] This invention provides a veterinary taurine composition for regulating the body's immunity through scientific compounding. The taurine, chicoric acid, and glycyrrhizic acid in the formula form a more effective synergistic effect in regulating the body's immunity, resulting in a more comprehensive efficacy and function. The three components in the formula, combined in a certain proportion, exhibit a significant synergistic effect in treating and regulating the body's immunity. Reducing or altering the components significantly reduces the immune-enhancing activity of this veterinary taurine composition for regulating the body's immunity.

[0023] This invention uses feed-grade raw materials, has a simple preparation method, is safe to use, leaves no harmful residues, and does not cause environmental pollution or drug resistance. It can be used to replace antibiotics, making it very suitable for large-scale industrial production and application, and has broad clinical prospects. Attached Figure Description

[0024] Figure 1 For comparison of IgA levels in the serum of fattening cattle, note: compared with the CG group, P <0.05, P <0.01; compared with group A, # P <0.05, ## P<0.01.

[0025] Figure 2 For comparison of IgG content in the serum of fattening cattle, note: compared with the CG group, P <0.05, P <0.01; compared with group A, # P <0.05, ## P <0.01.

[0026] Figure 3 For comparison of IgM levels in the serum of fattening cattle, note: compared with the CG group, P <0.05, P <0.01; compared with group A, # P <0.05, ## P <0.01.

[0027] Figure 4 For comparison of IgA levels in the serum of fattening pigs, note: compared with the CG group, P <0.05, P <0.01; compared with group A, # P <0.05, ## P <0.01.

[0028] Figure 5 For comparison of IgG content in the serum of fattening pigs, note: compared with the CG group, P <0.05, P <0.01; compared with group A, # P <0.05, ## P <0.01.

[0029] Figure 6 For comparison of IgM levels in the serum of fattening pigs, note: compared with the CG group, P <0.05, P <0.01; compared with group A, # P <0.05, ## P <0.01. Detailed Implementation

[0030] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0031] Furthermore, to better illustrate the present invention, numerous specific details are provided in the following detailed embodiments. Those skilled in the art should understand that the present invention can be practiced without certain specific details. In some embodiments, materials, elements, methods, and means well known to those skilled in the art are not described in detail in order to highlight the spirit of the invention.

[0032] In the following examples, all raw materials used are commercially available products that can be purchased directly or can be prepared using conventional techniques in the field.

[0033] The preparation methods in Examples 1-7 below are as follows: each raw material component is weighed according to the formula components; the weighed components are mixed to obtain the veterinary taurine powder that regulates the body's immunity.

[0034] Example 1 A veterinary taurine powder for regulating the body's immunity is composed of taurine, chicoric acid, and glycyrrhizin in a mass ratio of 3:0.5:1.

[0035] Example 2 A veterinary taurine powder for regulating the body's immunity is composed of taurine, chicoric acid, and glycyrrhizin in a mass ratio of 4:0.5:1.5.

[0036] Example 3 A veterinary taurine powder for regulating the body's immunity is composed of taurine, chicoric acid, and glycyrrhizin in a mass ratio of 3:1:1.

[0037] Example 4 A veterinary taurine powder for regulating the body's immunity is composed of taurine, chicoric acid, and glycyrrhizin in a mass ratio of 3:1:1.5.

[0038] Example 5 A veterinary taurine powder for regulating the body's immunity is composed of taurine, chicoric acid, and glycyrrhizin in a mass ratio of 4:1:1.5.

[0039] Example 6 A veterinary taurine powder for regulating the body's immunity is composed of taurine, chicoric acid, and glycyrrhizic acid in a mass ratio of 4:1:1.

[0040] Example 7 A veterinary taurine powder for regulating the body's immunity is composed of taurine, chicoric acid, and glycyrrhizic acid in a mass ratio of 3:0.5:1.5.

[0041] The preparation methods in Comparative Examples 1-6 are as follows: each raw material component is weighed according to the formula composition; the weighed components are mixed to obtain the powder that regulates the body's immunity.

[0042] Comparative Example 1 A powder for regulating the body's immunity is composed of chicoric acid and licorice polysaccharide in a mass ratio of 0.5:1.

[0043] Compared with Example 1, the difference is that it does not contain taurine, while the other components and their amounts remain the same.

[0044] Comparative Example 2 A powder that regulates the body's immunity is composed of taurine and glycyrrhiza polysaccharide in a mass ratio of 3:1.

[0045] Compared with Example 1, the difference is that it does not contain chicoric acid, while the other components and amounts remain the same.

[0046] Comparative Example 3 A powder for regulating the body's immunity is composed of taurine and chicoric acid in a mass ratio of 3:0.5.

[0047] Compared with Example 1, the difference is that it does not contain licorice polysaccharide, while the other components and amounts remain the same.

[0048] Comparative Example 4 A powder for regulating the body's immunity is composed of taurine, chlorogenic acid and glycyrrhizin in a mass ratio of 3:0.5:1.

[0049] The difference from Example 1 is that chlorogenic acid is used instead of chicoric acid.

[0050] Comparative Example 5 A powder for regulating the body's immunity is composed of taurine, chicoric acid and angelica polysaccharide in a mass ratio of 3:0.5:1.

[0051] The difference from Example 1 is that Angelica polysaccharide is used instead of Glycyrrhiza polysaccharide.

[0052] Comparative Example 6 A powder for regulating the body's immunity is composed of taurine, chicoric acid and glycyrrhizin in a mass ratio of 2:3:4.

[0053] The difference compared to Example 1 is that the amount of each component is changed.

[0054] The effect of the veterinary taurine powder of this invention on the immune function of fattening cattle 1. Experimental Methods This experiment was conducted at a beef cattle farm. Eighty healthy fattening cattle weighing approximately 400 kg were selected and randomly divided into 10 groups of 8 cattle each. The groups were: control group (CG group), Example 1 group (A group), Example 2 group (B group), Example 3 group (C group), Comparative example 1 group (D group), Comparative example 2 group (E group), Comparative example 3 group (F group), Comparative example 4 group (G group), Comparative example 5 group (H group), and Comparative example 6 group (I group).

[0055] Group CG was fed a basal diet. The experimental groups were fed a basal diet supplemented with the corresponding products. Group A was fed a basal diet supplemented with 45 mg / kg of Example 1 powder, Group B was fed a basal diet supplemented with 45 mg / kg of Example 2 powder, Group C was fed a basal diet supplemented with 45 mg / kg of Example 3 powder, Group D was fed a basal diet supplemented with 45 mg / kg of Comparative Example 1 powder, Group E was fed a basal diet supplemented with 45 mg / kg of Comparative Example 2 powder, Group F was fed a basal diet supplemented with 45 mg / kg of Comparative Example 3 powder, Group G was fed a basal diet supplemented with 45 mg / kg of Comparative Example 4 powder, Group H was fed a basal diet supplemented with 45 mg / kg of Comparative Example 5 powder, and Group I was fed a basal diet supplemented with 45 mg / kg of Comparative Example 6 powder.

[0056] According to the "Beef Cattle Feeding Standard" (NY / T 815-2004), the basic feed for fattening cattle should be formulated based on their nutritional requirements. The main concentrates of the basic feed should be corn, wheat bran, soybean meal and cottonseed meal, while the roughage should be whole-plant silage corn, wheat straw and corn stalks.

[0057] This experiment selected a pre-feeding period of 10 days and an experimental period of 60 days, and the feeding amount was recorded on time.

[0058] Before feeding on the morning following the end of the feeding trial, blood was collected from the tail vein using vacuum blood collection tubes. 20 mL was collected from each pig and left to stand in a 4°C refrigerator for 2 hours. The blood was then centrifuged at 1000×g for 10 minutes, and the upper serum layer was collected. The serum was then quickly frozen in liquid nitrogen and sent to the laboratory for storage at -80°C for later use.

[0059] The levels of serum immune factors IgA, IgM, and IgG were determined using an enzyme-linked immunosorbent assay (ELISA).

[0060] 2. Statistical Analysis Experimental data were statistically analyzed using GraphPad Prism 8.0 software, expressed as mean ± standard deviation. The results are expressed as mean ± standard deviation (±s), and one-way ANOVA was used for comparisons between groups.

[0061] 3. Experimental Results The effect of the veterinary taurine powder of this invention on the content of immune factors in the serum of fattening cattle As shown in Table 1, Figure 1 , Figure 2 , Figure 3 As shown, compared with the CG group, the serum levels of IgA, IgG, and IgM in each treatment group were significantly increased. The serum levels of IgA, IgG, and IgM in group A were significantly higher than those in group DI. This indicates that the taurine powder of this invention can effectively enhance the humoral immunity of fattening cattle.

[0062] Table 1. Effects of the herbal composition of the present invention on the serum immune factor content of fattening cattle. Note: Compared with the CG group, P <0.05, P <0.01; compared with group A, # P <0.05, ## P <0.01.

[0063] Immunoglobulin levels are important indicators for assessing an animal's immune status and health. IgA, IgM, and IgG, as three core immunoglobulins, work synergistically to construct a multi-layered immune defense system. This invention's veterinary taurine powder significantly increases the levels of IgA, IgG, and IgM in serum, effectively enhancing the immunity of fattening cattle.

[0064] Compared to Example 1 of the present invention, Comparative Example 1 does not contain taurine, and its effect on enhancing the body's immunity is significantly weakened. Comparative Example 2, compared to Example 1 of the present invention, does not contain chicoric acid, and its effect on enhancing the body's immunity is significantly weakened. Comparative Example 3, compared to Example 1 of the present invention, does not contain glycyrrhizin, and its effect on enhancing the body's immunity is significantly weakened. The difference between Comparative Examples 4 and 5 and Example 1 is that chicoric acid and glycyrrhizin were replaced with other substances in equal amounts. The effect of Comparative Examples 4 and 5 on enhancing the body's immunity is significantly worse than that of Example 1. Therefore, the combination of taurine, chicoric acid, and glycyrrhizin has special properties; only the combination of these three components can better enhance the body's immunity.

[0065] Compared with Example 1 of the present invention, Comparative Example 6 showed a significant decrease in the efficacy of enhancing the body's immunity by changing the dosage of each component. Therefore, it can be concluded that only when taurine, chicoric acid, and glycyrrhizic acid are combined in a specific ratio can they achieve a synergistic effect and better enhance the body's immunity.

[0066] This invention provides a veterinary taurine powder for enhancing the body's immunity. The formulation is rationally balanced, with each ingredient synergistically enhancing its effect, resulting in a more comprehensive efficacy and function. Taurine, chicoric acid, and glycyrrhizin in the formula exhibit significant synergistic effects in treating and enhancing the body's immunity. Reducing or altering the composition of the components significantly reduces the immune-enhancing effect.

[0067] The effect of the veterinary taurine powder of this invention on the immune function of fattening pigs 1. Experimental Methods This experiment was conducted at a pig farm. Forty-eight healthy fattening pigs of similar weight were selected and randomly divided into six groups of eight pigs each. The groups were: control group (CG group), Example 1 group (A group), comparative example 4 group (G group), comparative example 5 group (H group), comparative example 6 group (I group), and compound acid group (J group).

[0068] The CG group was fed a basal diet. The experimental groups were fed the basal diet supplemented with the corresponding products: Group A received 46 mg / kg of Example 1 powder, Group G received 46 mg / kg of Comparative Example 4 powder, Group H received 46 mg / kg of Comparative Example 5 powder, and Group I received 46 mg / kg of Comparative Example 6 powder. Compound acid was added at 2 g / kg to the basal diet. The compound acid consisted of lactic acid, citric acid, and phosphoric acid in a mass ratio of 3:1:6.

[0069] The composition of the basal diet is shown in Table 2.

[0070] Table 2. Composition of basal diet (air-dried basal) (%) The premix provides the following per kilogram of feed: Vitamin A 8500 IU, Vitamin D 2800 IU, Vitamin E 20 IU, Vitamin K 35 mg, Vitamin B1 4 mg, Vitamin B2 5.5 mg, Vitamin B6 3.5 mg, and Vitamin B2 4 mg. 12 0.28mg, Fe 80mg, Zn 100mg, Cu 35mg, Mn 60mg, I 0.3 mg, Se0.25mg.

[0071] The pigs have free access to feed and water. The pigsty is cleaned and disinfected daily to keep it dry and well-ventilated. The pigs are fed three times a day, and other feeding and management practices are carried out as usual.

[0072] This experiment selected a pre-feeding period of 10 days and an experimental period of 58 days, and the feeding amount was recorded on time.

[0073] On the morning following the end of the feeding trial, blood was collected from the tail vein using a vacuum blood collection tube. 10 mL was collected from each pig and left to stand in a 4°C refrigerator for 2 hours. The blood was then centrifuged at 3000×g for 5 minutes, and the upper serum layer was collected and stored at -20°C for later use.

[0074] The levels of serum immune factors IgA, IgM, and IgG were determined using an enzyme-linked immunosorbent assay (ELISA).

[0075] 2. Statistical Analysis Experimental data were statistically analyzed using GraphPad Prism 8.0 software, expressed as mean ± standard deviation. The results are expressed as mean ± standard deviation (±s), and one-way ANOVA was used for comparisons between groups.

[0076] 3. Experimental Results The effect of the veterinary taurine powder of this invention on the content of immune factors in the serum of fattening pigs like Figure 4 , Figure 5 , Figure 6 As shown, compared with group CG, the levels of IgA, IgG, and IgM in the serum of group A were significantly increased. The levels of IgA, IgG, and IgM in the serum of group A were significantly higher than those in groups G, H, and I. This indicates that the taurine powder of the present invention can effectively enhance the humoral immunity of fattening pigs.

[0077] The levels of IgA, IgG, and IgM in the serum of group A were significantly higher than those in group J, demonstrating more significant technical effects compared to existing products.

[0078] The veterinary taurine powder of this invention significantly increases the levels of IgA, IgG and IgM in serum, and can effectively enhance the immunity of fattening pigs.

[0079] The difference between Comparative Examples 4 and 5 and Example 1 is that chicoric acid and glycyrrhizin were replaced with other substances in equal amounts. The immune-enhancing effects of Comparative Examples 4 and 5 on fattening pigs were significantly worse than those of Example 1. Therefore, the combination of taurine, chicoric acid, and glycyrrhizin has unique properties; only the combination of these three components can better enhance the body's immunity.

[0080] Compared with Example 1 of the present invention, Comparative Example 6 showed a significant decrease in the efficacy of enhancing the immunity of fattening pigs by changing the dosage of each component. Therefore, it can be concluded that only when taurine, chicoric acid, and glycyrrhizic acid are combined in a specific ratio can they achieve a synergistic effect and better enhance the body's immunity.

[0081] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A veterinary taurine composition for regulating the body's immunity, characterized in that, The veterinary taurine composition for regulating the body's immunity is composed of taurine, chicoric acid, and glycyrrhizin.

2. The veterinary taurine composition for regulating the body's immunity according to claim 1, characterized in that, The veterinary taurine composition for regulating the body's immunity consists of taurine, chicoric acid, and glycyrrhizin in a mass ratio of 3-4:0.5-1:1-1.

5.

3. The veterinary taurine composition for regulating the body's immunity according to claim 2, characterized in that, The veterinary taurine composition for regulating the body's immunity consists of taurine, chicoric acid, and glycyrrhizin in a mass ratio of 3:0.5:

1.

4. The veterinary taurine composition for regulating the body's immunity according to claim 2, characterized in that, The veterinary taurine composition for regulating the body's immunity consists of taurine, chicoric acid, and glycyrrhizin in a mass ratio of 4:0.5:1.

5.

5. The veterinary taurine composition for regulating the body's immunity according to claim 2, characterized in that, The veterinary taurine composition for regulating the body's immunity consists of taurine, chicoric acid, and glycyrrhizin in a mass ratio of 3:1:

1.

6. A method for preparing a veterinary taurine composition for regulating the body's immunity, characterized in that, The process includes the following steps: weighing each raw material component according to any one of the formulation components described in claims 1-5; mixing the weighed components to obtain the veterinary taurine composition for regulating the body's immunity.

7. A veterinary traditional Chinese medicine preparation comprising the veterinary taurine composition according to any one of claims 1-5.

8. The veterinary traditional Chinese medicine preparation according to claim 7, characterized in that, The dosage form of the traditional Chinese medicine preparation is powder, ointment, or spray.

9. The veterinary traditional Chinese medicine preparation according to claim 8, characterized in that, The particle size of the powder is less than 160 mesh.

10. The use of the veterinary taurine composition for regulating the body's immunity as described in any one of claims 1-5, or the veterinary traditional Chinese medicine preparation as described in claim 7 or 8, in the preparation of veterinary drugs for regulating the body's immunity.

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