Soothing and repairing skin care composition as well as preparation method and application thereof

Abstract

The invention discloses a soothing and repairing skin care composition as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The skin care composition is prepared from the following components in parts by mass: 3 to 7 parts of herba centellae extract, 0.5 to 1 part of radix scutellariae extract, 2 to 4 parts of rhizoma polygoni cuspidati extract, 1 to 3 parts of radix gentianae extract and 15 to 20 parts of rhizoma atractylodis macrocephalae extract. Wherein the rhizoma atractylodis macrocephalae root extract is prepared by adopting a compound fermentation process of rhodotorula glutinis, lactobacillus brewer and lactobacillus paracasei, and active ingredients are richer. Through the synergistic effect of multiple components, the composition can significantly inhibit secretion of inflammatory factors IL-6 and activity of hyaluronidase and promote HaCaT cell migration, has excellent soothing, anti-inflammatory and repairing effects, and can effectively improve the problems of skin sensitivity, dryness and barrier injury.

Description

Technical Field

[0001] This invention belongs to the technical field of cosmetics, specifically relating to a soothing and repairing skincare composition, its preparation method, and its application. Background Technology

[0002] With environmental changes, increased life stress, and improper skincare habits, skin sensitivity, frequent inflammation, and damaged skin barriers are becoming increasingly common. Consumer demand for skincare products that combine soothing, anti-inflammatory, and repairing effects continues to rise. Current technologies for soothing and repairing skincare products often use single plant extracts or simple compound formulations, which suffer from poor ingredient synergy, limited efficacy, and low utilization of active ingredients. Some products rely on chemical anti-inflammatory ingredients, which may irritate the skin with long-term use. Furthermore, traditional plant extract preparation processes often involve simple extraction, failing to fully activate the active substances in the raw materials, resulting in limited soothing and repairing effects that fail to meet consumers' needs for highly effective and gentle skincare.

[0003] Therefore, developing a soothing and repairing skincare composition with synergistic components, outstanding activity, and safety and gentleness has significant market value. Summary of the Invention

[0004] To address the shortcomings of existing soothing and repairing skincare products, such as insufficient synergy of ingredients, low utilization rate of active ingredients, limited efficacy, and potential irritation, this invention aims to provide a soothing and repairing skincare composition. By screening five natural plant extracts and scientifically proportioning them, and optimizing the compound fermentation preparation process of Atractylodes macrocephala root extract, synergistic effects of each component are achieved. The result is a skincare composition with excellent anti-inflammatory, soothing, and skin barrier repair capabilities. This composition is safe and gentle, suitable for all skin types, especially sensitive skin.

[0005] To achieve the above objectives, the present invention discloses the following technical solutions: In a first aspect, the present invention provides a soothing and repairing skincare composition, wherein, by weight parts, the skincare composition contains the following components: Centella asiatica extract 3-7 parts; Scutellaria baicalensis root extract 0.5-1 part; Polygonum cuspidatum root extract 2-4 parts; 1-3 parts of gentian extract; 15-20 parts of Atractylodes macrocephala root extract.

[0006] Preferably, the skincare composition contains the following components in parts by weight: Centella asiatica extract 5 parts; 0.8 parts of Scutellaria baicalensis root extract; 3 portions of Polygonum cuspidatum root extract; Two portions of gentian extract; 17 portions of Atractylodes macrocephala root extract.

[0007] More preferably, the preparation method of the Atractylodes macrocephala root extract includes the following steps: Step 1: Crush the dried Atractylodes macrocephala root into 40-60 mesh to obtain Atractylodes macrocephala powder, then mix it with distilled water, add glucose, stir evenly and sterilize to obtain fermentation substrate; Step 2, First stage: Inoculate the Rhodotorula glutinis broth into the fermentation substrate at an inoculation rate of 5 v / v%, and ferment at 28-30℃ for 36-48 hours, with an aeration rate of 0.5-0.6 vvm during fermentation; Second stage: Mix the Lactobacillus sacchariformis broth and Lactobacillus paracasei broth, and add them to the first stage fermentation broth at an inoculation rate of 10 v / v%, and ferment at 37℃ for 72 hours, adjusting the aeration rate to 0.1-0.2 vvm during fermentation; Step 3: After fermentation, filter the fermentation liquid with a filter cloth to remove residue and collect the fermentation liquid. Sterilize the fermentation liquid, centrifuge, and concentrate under reduced pressure to obtain Atractylodes macrocephala root extract.

[0008] More preferably, the Lactobacillus sacchariformis has the accession number GDMCC 1.1769 and was purchased from the Guangdong Provincial Microbial Culture Collection Center; The Rhodopsinium glutamicum was obtained from Guangdong Provincial Microbial Culture Collection Center under the accession number GDMCC 2.155. The Lactobacillus paracasei was obtained from the Guangdong Provincial Microbial Culture Collection Center under the accession number GDMCC 1.159.

[0009] More preferably, the viable count of the *Lactobacillus sacchariformis* bacterial solution is 10-1. 8 CFU / mL; The viable count of the Rhodotorula glutinis culture was 10. 8 CFU / mL; The viable count of the Lactobacillus paracasei culture was 10. 8 CFU / mL.

[0010] More preferably, in step 1, the Atractylodes macrocephala powder and distilled water are mixed at a mass-to-volume ratio of 1:8 g / mL.

[0011] More preferably, the amount of glucose added in step 1 is 1% of the total mass of Atractylodes macrocephala powder and distilled water.

[0012] More preferably, in step 3, the fermentation broth is heated in a water bath at 80°C for 20 minutes, then centrifuged at 4°C and 8000 rpm for 20 minutes, the supernatant is collected, and the supernatant is concentrated under reduced pressure at 60°C to 1 / 3 of the original volume to obtain Atractylodes macrocephala root extract.

[0013] Secondly, the present invention provides the application of the skin care composition described in the first aspect in the preparation of skin care products with soothing, anti-inflammatory and repairing effects.

[0014] Preferably, the skin care product includes creams, ointments, liquids, lotions, gels, and / or sprays.

[0015] Preferably, the skin care product includes rinse-off preparations and / or leave-on preparations.

[0016] Preferably, the amount of the skincare composition added to the skincare product is 0.5-1 wt%.

[0017] In this invention Centella asiatica extract, as a core anti-inflammatory and repairing ingredient, can inhibit the release of inflammatory factors, promote the synthesis of collagen in the skin, enhance the skin barrier's resilience, and relieve skin redness and sensitivity.

[0018] Scutellaria baicalensis root extract has significant antibacterial and anti-inflammatory activity, which can inhibit the growth of harmful microorganisms, reduce skin inflammation, and help stabilize skin condition.

[0019] Polygonum cuspidatum root extract is rich in antioxidants, which can eliminate free radicals, reduce damage to the skin from external stimuli, and at the same time enhance anti-inflammatory effects and soothe skin discomfort.

[0020] Gentian extract has calming and soothing effects, which can relieve burning and stinging sensations caused by skin sensitivity, regulate skin tolerance, and improve skin sensitivity.

[0021] After being fermented by a combination of Rhodotorula glutinis, Lactobacillus sacchariformis, and Lactobacillus paracasei, the active ingredients of Atractylodes macrocephala root extract are fully activated, which can enhance the skin's moisturizing ability, inhibit hyaluronidase activity to reduce moisture loss, and at the same time, work synergistically with other ingredients to enhance anti-inflammatory and repair effects. It is the core repair and synergistic ingredient in the composition.

[0022] The beneficial effects of this invention are: 1. This invention achieves excellent anti-inflammatory, soothing, and repairing effects through a scientific ratio of five plant extracts: Centella asiatica extract, Scutellaria baicalensis root extract, Polygonum cuspidatum root extract, Gentiana macrophylla extract, and Atractylodes macrocephala root extract. This is superior to compound solutions with single ingredients or those lacking any of the ingredients. 2. The skin care composition provided by this invention can significantly promote HaCaT cell migration, quickly repair skin barrier damage, improve skin dryness and sensitivity, and has outstanding repair capabilities.

[0023] 3. The skin care composition provided by this invention can be added to various skin care formulations such as creams, ointments, water, lotions, gels, and sprays, covering both rinse-off and leave-on preparations, and is suitable for different skin care scenarios. Detailed Implementation

[0024] Those skilled in the art can refer to the content of this document to appropriately improve the process parameters. It should be particularly noted that all similar substitutions and modifications are obvious to those skilled in the art and are considered to be included in this invention.

[0025] The products and methods of the present invention have been described through preferred embodiments. Those skilled in the art will be able to make modifications or appropriate alterations and combinations to the products and methods described herein without departing from the content, spirit and scope of the present invention, so as to realize and apply the technology of the present invention.

[0026] Experimental methods in the following examples, unless otherwise specified, are generally performed under standard conditions or as recommended by the manufacturer. Materials, reagents, etc., used are commercially available unless otherwise specified. Unless otherwise stated, substances described in this invention are calculated as percentages and parts by mass.

[0027] Atractylodes macrocephala: The root of Atractylodes macrocephala, a plant belonging to the genus Atractylodes in the family Asteraceae, is commercially available. Lactobacillus sacchariformis: accession number GDMCC 1.1769, purchased from Guangdong Provincial Microbial Culture Collection Center; Rhodotorula glutinis: accession number GDMCC 2.155, purchased from Guangdong Provincial Microbial Culture Collection Center; Lactobacillus paracasei: accession number GDMCC 1.159, purchased from Guangdong Provincial Microbial Culture Collection Center;

[0028] Step 1: Activation of microbial strains ① Streak Rhodotorula glutinis on YPD agar plates and incubate at 28℃ for 48 hours. Pick a single colony and inoculate it into YPD liquid medium. Incubate at 28℃ with shaking at 180 rpm for 24-36 hours until the bacterial concentration reaches 10. 8 The concentration of approximately CFU / mL yielded a solution of *Rhodotorula glutinis*.

[0029] ② Streak *Lactobacillus sacchari* and *Lactobacillus paracasei* cultures onto MRS agar plates and incubate at 37°C for 48 hours. Single colonies are then picked and inoculated into MRS liquid medium and cultured at 37°C with shaking at 100 rpm for 18-24 hours, until the bacterial concentration reaches 10⁻⁶. 8 CFU / mL was used to obtain Lactobacillus sacchariformis and Lactobacillus paracasei bacterial cultures, respectively.

[0030] Step 2: Fermentation substrate treatment The dried Atractylodes macrocephala root was pulverized to 40-60 mesh to obtain Atractylodes macrocephala powder. Then, it was mixed with distilled water at a ratio of 1:8 (w / v, g / mL), and 1% glucose was added. After stirring evenly, it was sterilized by high pressure steam at 121℃ for 30 minutes to obtain the fermentation substrate, which was then cooled for later use.

[0031] Step 3: Fermentation treatment First stage: Inoculate the activated Rhodotorula glutinis broth into the cooled fermentation substrate at an inoculation rate of 5 v / v%, and ferment at 28-30℃ for 36-48 hours. The aeration rate during fermentation is 0.5-0.6 vvm. Second stage: The activated Lactobacillus sacchariformis culture and Lactobacillus paracasei culture were mixed at a volume ratio of 1:1 and added to the first stage fermentation broth at an inoculation rate of 10 v / v%. Fermentation was carried out at 37℃ for 72 hours, and the aeration rate was adjusted to 0.1-0.2 vvm during the fermentation process.

[0032] Step 4, Post-processing After fermentation, the residue was first removed by filtering with a 200-mesh filter cloth, and the fermentation broth was collected. The fermentation broth was heated in an 80℃ water bath for 20 minutes to inactivate microorganisms and enzymes. Then, it was centrifuged at 4℃ and 8000 rpm for 20 minutes, and the supernatant was collected. The bacterial cells and insoluble matter were discarded, and the supernatant was concentrated under reduced pressure at 60℃ to 1 / 3 of the original volume to obtain Atractylodes macrocephala root extract.

[0033] I. Compositions 1-3 According to the mass ratio in Table 1, the soothing and repairing skin care compositions 1-3 were prepared. Table 1. Mass ratio of each raw material in compositions 1-3 Raw material name Composition 1 Composition 2 Composition 3 Centella Asiatica extract 7 5 3 Scutellaria baicalensis root extract 0.5 0.8 1 Polygonum cuspidatum root extract 2 3 4 Gentian extract 1 2 3 Atractylodes macrocephala root extract 15 17 20 II. Composition 4-9 Compositions 4-9 were obtained by adjusting the formulation of composition 2, as shown in Table 2. Table 2. Mass ratio of each raw material in compositions 4-9 Raw material name Composition 4 Composition 5 Composition 6 Composition 7 Composition 8 Composition 9 Centella Asiatica extract / 5 5 5 / 5 Scutellaria baicalensis root extract 0.8 / 0.8 0.8 / 0.8 Polygonum cuspidatum root extract 3 3 / 3 / 3 Gentian extract 2 2 2 / / 2 Atractylodes macrocephala root extract 17 17 17 17 17 / III. Composition 10-11 Composition 10: Based on the formulation of Composition 2, replace Atractylodes macrocephala root extract with Atractylodes macrocephala root extract ①. The preparation method of Atractylodes macrocephala root extract ① is as follows: refer to the preparation method of Atractylodes macrocephala root extract, except that the Rhodotorula glutinis liquid in step 3 is replaced with Rhodotorula glutinis (ACCC 21436, purchased from China Agricultural Microbial Culture Collection Center) liquid, with a viable count of 10. 8 CFU / mL, all other values ​​remain unchanged; Composition 11: Based on the formulation of Composition 2, replace Atractylodes macrocephala root extract with Atractylodes macrocephala root extract ②. The preparation method of Atractylodes macrocephala root extract ② is the same as that of Atractylodes macrocephala root extract, except that the second stage in step 3 is adjusted to fermentation treatment with Lactobacillus sacchariculture liquid alone, and the rest remains unchanged. Composition 12: Based on the formulation of Composition 2, replace Atractylodes macrocephala root extract with Atractylodes macrocephala root extract ③. The preparation method of Atractylodes macrocephala root extract ③ is as follows: refer to the preparation method of Atractylodes macrocephala root extract, except that the second stage in step 3 is adjusted to fermentation treatment with Lactobacillus paracasei liquid alone, and the rest remains unchanged. The specifics are shown in Table 3.

[0034] Table 3. Mass ratio of each raw material in composition 10-12 Raw material name Composition 10 Composition 11 Composition 12 Centella Asiatica extract 5 5 5 Scutellaria baicalensis root extract 0.8 0.8 0.8 Polygonum cuspidatum root extract 3 3 3 Gentian extract 2 2 2 Atractylodes macrocephala root extract ① 17 / / Atractylodes macrocephala root extract ② / 17 / Atractylodes macrocephala root extract ③ / / 17

[0035] I. Experimental Materials 1.1 Test Sample Compositions 1-12.

[0036] 1.2 Cells and Reagents Cells: Mouse macrophages (RAW264.7) and human immortalized epidermal cells (HaCaT) were purchased from BeiNa Biotechnology Co., Ltd.

[0037] Reagents: LPS (lipopolysaccharide), dexamethasone (positive control), MTT, DMSO, PBS buffer, DMEM basal medium, fetal bovine serum (FBS), IL-6 ELISA kit, hyaluronidase, sodium hyaluronate, acetate buffer (pH=5.6), CaCl2, NaOH, acetylacetone, P-DAB reagent, potassium glycyrrhizate (positive control).

[0038] II. Experimental Methods 2.1 MTT Experiment Cell seeding: RAW264.7 cells in logarithmic growth phase were adjusted to a density of 8 × 10⁻⁶ cells. 4 The fractions were inoculated at a rate of 1 / mL into 200 μL per well of a 96-well plate and incubated at 37°C with 5% CO2 for 24 h.

[0039] Test substance treatment: The composition was diluted 1-12 with culture medium to set 5 gradients: 0.005%, 0.01%, 0.02%, 0.05%, and 0.1%.

[0040] Set up groups: Set up blank control group (cells + culture medium), zeroing well (cell-free + culture medium), and test group (cells + test solution), with 3 replicates for each group.

[0041] Culture and detection: After adding the test solution, continue to culture for 24 h. Add 20 μL of MTT working solution (5 mg / mL) to each well, incubate for 4 h, discard the supernatant, add 150 μL of DMSO and shake for 10 min. Measure the absorbance (OD value) at 490 nm using a microplate reader.

[0042] Cell viability calculation: Cell viability % = (OD value of experimental group - OD value of zeroing well) / (OD value of blank control group - OD value of zeroing well) × 100%. Viability ≥ 90% is considered a safe working concentration.

[0043] Determination of safe working concentration: When the concentration is ≤0.1%, the cell viability is >90%, and the concentration of 0.1% is determined as the working concentration for subsequent cell experiments.

[0044] 2.2 RAW264.7 cell IL-6 secretion inhibition experiment Cell seeding: RAW264.7 cells were adjusted to a density of 8.0 × 10⁻⁶. 4 The fractions were inoculated at a rate of 1 / mL into 200 μL per well of a 96-well plate and incubated at 37°C with 5% CO2 for 24 h.

[0045] Grouping was set up as follows: blank control group (no cells + culture medium), negative control group (cells + LPS + culture medium), positive control group (cells + LPS + 100 μg / mL dexamethasone), and test group (cells + LPS + 0.1% test solution), with 4 replicates for each group.

[0046] Induction and culture: After incubation with the test solution for 1 h, LPS (final concentration 1 μg / mL) was added to each well to induce inflammation, and culture was continued for 24 h.

[0047] Detection: Collect cell supernatant, centrifuge at 1000 rpm for 10 min, and use an ELISA kit to detect the specific concentration of IL-6, strictly following the kit instructions.

[0048] Inhibition rate calculation: Inhibition rate (%) = [(IL-6 concentration in negative control group - IL-6 concentration in test group) / (IL-6 concentration in negative control group - IL-6 concentration in blank control group)] × 100%.

[0049] 2.3 Hyaluronidase Activity Inhibition Experiment Sample preparation: Set up 4 sets of test tubes (A, B, C, D). Add 50 μL of 0.5% test solution to tubes A and B (dilute composition 1-12 to 0.5% with buffer solution respectively), add 50 μL of distilled water to tubes C and D; add 50 μL of 500 U / mL hyaluronidase solution to tubes A and C, and add 50 μL of acetate buffer to tubes B and D. Incubate at 37℃ for 20 min.

[0050] Activation and substrate reaction: Add 10 μL of 2.5 mol / L CaCl2 solution to each tube and incubate at 37℃ for 20 min; add 50 μL of 0.5 mg / mL sodium hyaluronate solution to tubes A and C, and add 50 μL of acetate buffer to tubes B and D, incubate at 37℃ for 40 min, and then let stand at room temperature for 10 min.

[0051] Color development and detection: Add 50 μL of distilled water, 10 μL of 5 mol / L NaOH solution, and 50 μL of acetylacetone solution to each tube. Boil in water for 30 min, then in an ice bath for 10 min, and let stand at room temperature for 10 min. Add 100 μL of P-DAB reagent and measure the absorbance (OD value) at 530 nm using an ELISA reader.

[0052] Inhibition rate calculation: Inhibition rate (%) = [(CD) - (AB)] / (CD) × 100% (A = OD value of sample, B = OD value of blank sample, C = OD value of control, D = OD value of blank control).

[0053] 2.4 Cell migration experiment 2.4.1 Test Sample Two concentration groups were set up using composition 2: 50 μg / mL and 100 μg / mL, wherein the solvent was EGF-free keratinocyte culture medium containing DMSO, and the final concentration of DMSO was ≤0.1%; Solvent control group: EGF-free keratinocyte culture medium containing only 0.1% DMSO.

[0054] 2.4.2 Test Method Inoculation: Adjust the density of HaCaT cells in logarithmic growth phase to 5 × 10⁻⁶. 5 Inoculate 2 mL per well into a 6-well plate and incubate overnight at 37°C with 5% CO2 until the cell layering rate reaches 80%.

[0055] Solution preparation: Prepare the solvent control group and test solutions of various concentrations of composition 2 according to the experimental design, with 3 replicates for each group.

[0056] Scratching: Using a sterile 200μL pipette tip perpendicular to the surface of the well plate, make three parallel scratches in each well of the cell layer, ensuring that the scratches are of uniform width.

[0057] Washing: Gently wash 3 times with PBS to remove dead cells and cell debris caused by the scratches.

[0058] Drug administration and culture: Add 2 mL of the corresponding test solution to each well (add an equal amount of solvent to the solvent control group), and incubate at 37°C in a 5% CO2 incubator for 24 h.

[0059] Photography: Images of the scratched areas of each well were taken at 0h and 24h after scratching using an inverted microscope under 4x magnification (three different fields of view were selected for each well).

[0060] Data analysis: Use Image Pro Plus software to measure scratch width and calculate migration rate and relative migration rate. III. Experimental Data Table 4 Results of the Influence of Inflammatory Factors Group IL-6 concentration (pg / mL) Inhibition rate / % Blank control 12.26±2.31 / negative control 320.56±10.25 / Positive control 135.67±4.52 59.97% Composition 1 167.32±5.89 49.70% Composition 2 147.25±4.31 56.21% Composition 3 159.67±6.23 52.19% Composition 4 224.39±8.79 31.19% Composition 5 215.88±8.21 33.95% Composition 6 208.51±7.98 36.34% Composition 7 202.14±7.56 38.41% Composition 8 274.89±9.67 14.81% Composition 9 248.76±6.54 23.29% Composition 10 172.45±5.98 48.04% Composition 11 182.34±6.12 44.83% Composition 12 179.38±6.05 45.79% Table 5 Hyaluronidase Inhibition Rate Group Inhibition rate (%) Blank control / negative control 0.00 Positive control 66.57 Composition 1 73.75 Composition 2 77.61 Composition 3 71.73 Composition 4 46.86 Composition 5 49.58 Composition 6 51.94 Composition 7 54.34 Composition 8 27.61 Composition 9 31.49 Composition 10 63.43 Composition 11 69.55 Composition 12 62.34 Table 6 Effects on cell migration Group Migration rate (%) Relative migration rate (%) P-value (vs solvent control) Solvent control group (DMSO) 12.09±0.87 100.00±3.56 / Composition 2 (50 μg / mL) 20.62±1.05 170.56±4.21 ＜0.05 Composition 2 (100 μg / mL) 35.92±1.23 297.11±5.17 ＜0.01 IV. Results Analysis IL-6 is a core factor mediating skin inflammation, and its decreased concentration directly reflects the anti-inflammatory and soothing ability of the test substance. According to Table 4, after LPS induction, the IL-6 concentration in the negative control group significantly increased to 320.56 pg / mL, while the positive control group showed an IL-6 concentration reduced to 135.67 pg / mL, verifying the effectiveness of the experimental system. Among compositions 1-3, composition 2 exhibited the highest IL-6 inhibition rate, indicating that its formulation maximizes the synergistic anti-inflammatory effect. The IL-6 inhibition rates of compositions 4-9 showed varying degrees of decrease compared to composition 2, demonstrating that the five raw materials provided in this invention—Centella asiatica extract, Scutellaria baicalensis root extract, Polygonum cuspidatum root extract, Gentiana scabra extract, and Atractylodes macrocephala root extract—are indispensable, and their combined use has a certain synergistic effect in anti-inflammation. The Atractylodes macrocephala root extracts in compositions 10-12 were obtained through different preparation processes, and their anti-inflammatory properties were not as good as those in composition 2. This proves that the Atractylodes macrocephala root extract obtained by the compound fermentation of specific strains Rhodotorula glutinis, Lactobacillus sacchariformis and Lactobacillus paracasei in this invention has richer anti-inflammatory active ingredients and better effects.

[0061] Overactivation of hyaluronidase degrades hyaluronic acid in the skin, leading to dryness and sensitivity. Its inhibition rate is a core evaluation indicator of soothing efficacy. According to Table 5, composition 2 showed the highest inhibition rate at a concentration of 0.5%, superior to other compositions, indicating that the optimal formulation can effectively inhibit hyaluronidase activity, reduce skin moisture loss, and provide a significant soothing effect. Compositions 4-9 exhibited inhibition rates ranging from 27.61% to 54.34%, verifying the synergistic soothing effect of all five ingredients. Compositions 10-12 showed lower inhibition rates than composition 2, consistent with the IL-6 experimental results, demonstrating that the specific compound microbial fermentation process provided by this invention can maximize the soothing activity of Atractylodes macrocephala root extract.

[0062] Cell migration rate is a core indicator reflecting skin repair efficacy. HaCaT cells, as epidermal keratinocytes, have a migration ability directly related to the repair speed after skin damage. As shown in Table 6, the composition provided by this invention has outstanding repair efficacy, exhibiting a certain concentration dependence; higher concentrations result in better barrier repair of the skin.

[0063] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A soothing and repairing skincare composition, characterized in that, The skincare composition contains the following components in parts by weight: Centella asiatica extract 3-7 parts; Scutellaria baicalensis root extract 0.5-1 part; Polygonum cuspidatum root extract 2-4 parts; 1-3 parts of gentian extract; 15-20 parts of Atractylodes macrocephala root extract.

2. The skincare composition according to claim 1, characterized in that, The skincare composition contains the following components in parts by weight: Centella asiatica extract 5 parts; 0.8 parts of Scutellaria baicalensis root extract; 3 portions of Polygonum cuspidatum root extract; Two portions of gentian extract; 17 portions of Atractylodes macrocephala root extract.

3. The skincare composition according to claim 2, characterized in that, The preparation method of the Atractylodes macrocephala root extract includes the following steps: Step 1: Crush the dried Atractylodes macrocephala root into 40-60 mesh to obtain Atractylodes macrocephala powder, then mix it with distilled water, add glucose, stir evenly and sterilize to obtain fermentation substrate; Step 2, First stage: Inoculate the Rhodotorula glutinis broth into the fermentation substrate at an inoculation rate of 5 v / v%, and ferment at 28-30℃ for 36-48 hours, with an aeration rate of 0.5-0.6 vvm during fermentation; Second stage: Mix the Lactobacillus sacchariformis broth and Lactobacillus paracasei broth, and add them to the first stage fermentation broth at an inoculation rate of 10 v / v%, and ferment at 37℃ for 72 hours, adjusting the aeration rate to 0.1-0.2 vvm during fermentation; Step 3: After fermentation, filter the fermentation liquid with a filter cloth to remove residue and collect the fermentation liquid. Sterilize the fermentation liquid, centrifuge, and concentrate under reduced pressure to obtain Atractylodes macrocephala root extract.

4. The skincare composition according to claim 3, characterized in that, In step 1, Atractylodes macrocephala powder and distilled water are mixed at a mass-volume ratio of 1:8 g / mL.

5. The skincare composition according to claim 3, characterized in that, In step 1, the amount of glucose added is 1% of the total mass of Atractylodes macrocephala powder and distilled water.

6. The skincare composition according to claim 3, characterized in that, In step 3, the fermentation broth is heated in a water bath at 80°C for 20 minutes, and then centrifuged at 4°C and 8000 rpm for 20 minutes. The supernatant is collected and concentrated under reduced pressure at 60°C to 1 / 3 of the original volume to obtain Atractylodes macrocephala root extract.

7. The use of the skin care composition according to any one of claims 1-6 in the preparation of skin care products with soothing, anti-inflammatory and repairing effects.

8. The application according to claim 7, characterized in that, The skincare products include creams, ointments, lotions, emulsions, gels, and / or sprays.

9. The application according to claim 7, characterized in that, The skin care products include rinse-off preparations and / or leave-on preparations.

10. The application according to claim 7, characterized in that, The amount of the skincare composition added to the skincare product is 0.5-1 wt%.