Indoor air virus rapid detection system and method

Abstract

The invention discloses an indoor air virus rapid detection system, which comprises: a trapping net membrane system, which comprises a trapping net membrane, a net membrane fixing assembly and a net membrane overturning assembly; the flushing system is used for being in butt joint with the overturned omentum fixing assembly and eluting viruses; the micro-fluidic system comprises a micro-fluidic chip, a sample inlet, a plurality of detection chambers and a micro-channel assembly, a virus specific recognition probe is fixed on the inner wall of each detection chamber, and the sample inlet is communicated with the flushing system; the electrochemical detection system is used for generating an electrochemical luminescence signal; the signal acquisition and amplification unit is used for capturing the electrochemical luminescence signal, converting the electrochemical luminescence signal into an electric signal and amplifying the electric signal; and the control system is respectively connected with the trapping net film system, the flushing system, the microfluidic system, the electrochemical detection system and the signal acquisition and amplification unit. The invention also discloses an indoor air virus rapid detection method. According to the invention, efficient, rapid and multi-target parallel detection of air viruses in an indoor environment is realized.

Description

Technical Field

[0001] This invention relates to the field of microbial detection technology, and in particular to a rapid indoor air virus detection system and method. Background Technology

[0002] Currently, airborne viruses such as influenza, COVID-19, and respiratory syncytial virus pose a persistent threat to public health. These viruses spread in indoor environments via aerosols, making early and rapid detection crucial for prevention and control. However, existing airborne virus detection technologies have several shortcomings: First, sampling relies on independent air pumps and other equipment, requiring separate sampling systems, resulting in bulky and costly equipment that is difficult to integrate into everyday scenarios such as homes and offices. Second, their virus enrichment efficiency is limited, and their ability to capture low-concentration viral aerosols is insufficient, making their detection sensitivity inadequate for early monitoring needs. Third, their detection modes are limited, with most systems only capable of detecting one type of virus, failing to address situations where multiple respiratory viruses co-circulate. Fourth, their operation procedures are complex, requiring professional personnel, and cross-contamination is prone to occur during sampling and testing. Fifth, their detection cycles are long, typically requiring several hours for integrated equipment and several days to obtain a test report, making real-time rapid monitoring impossible. Summary of the Invention

[0003] In view of the shortcomings of existing technologies, the purpose of this invention is to provide a rapid indoor air virus detection system and method.

[0004] To achieve the above objectives, an embodiment of the present invention provides the following technical solution:

[0005] An indoor air virus rapid detection system includes:

[0006] A net film catching system includes a net film catching device, a net film fixing component, and a net film flipping component. The net film catching device is disposed on the net film fixing component, and the net film flipping component is connected to the net film fixing component and drives the net film fixing component to flip.

[0007] A flushing system is used to dock with the flipped mesh fixing assembly and to wash away the viruses captured by the trapping mesh;

[0008] A microfluidic system, comprising a microfluidic chip, an inlet disposed on the microfluidic chip, multiple detection chambers, and a microchannel assembly connecting the inlet and the multiple detection chambers, wherein a virus-specific recognition probe is fixed on the inner wall of each detection chamber, and the inlet is connected to the flushing system;

[0009] Electrochemical detection system, used to generate electrochemiluminescence signals;

[0010] The signal acquisition and amplification unit is used to capture the electrochemiluminescence signal and convert it into an electrical signal, and amplify the electrical signal;

[0011] The control system is connected to the trapping mesh system, the rinsing system, the microfluidic system, the electrochemical detection system, and the signal acquisition and amplification unit, respectively.

[0012] As a further improvement of the present invention, the capturing mesh includes multiple mesh blocks, and the mesh fixing assembly includes a detachable adapter plate and multiple adjustable buckles, wherein the multiple mesh blocks are disposed on the detachable adapter plate by the multiple adjustable buckles.

[0013] As a further improvement of the present invention, the mesh block is made of PP meltblown fabric.

[0014] As a further improvement of the present invention, the mesh block has a size of 2cm×2cm and a pore size of 1-5 micrometers.

[0015] As a further improvement of the present invention, the mesh flipping assembly includes a stepper motor and a positioning sensor, the output end of the stepper motor is connected to a drive shaft, the mesh fixing assembly is connected to the drive shaft, and the positioning sensor is disposed in the rinsing system.

[0016] As a further improvement of the present invention, the rinsing system includes a rinsing chamber, a spray assembly, a rinsing fluid storage tank, and a guide pipe. The spray assembly includes a rotating telescopic arm disposed at the top edge of the rinsing chamber and a spray head connected to the rotating telescopic arm. A rinsing pipe is connected between the rinsing fluid storage tank and the rinsing chamber. A first micro peristaltic pump is disposed on the rinsing pipe. The guide pipe is connected to the bottom of the rinsing chamber and the sample inlet, respectively. A second micro peristaltic pump is disposed on the guide pipe.

[0017] As a further improvement of the present invention, the microfluidic system further includes a buffer storage bottle and multiple waste liquid chambers. The buffer storage bottle is connected to the guide tube through a buffer tube. A third micro peristaltic pump is provided on the buffer tube. The waste liquid chambers are located inside the microfluidic chip and are connected to the detection chamber.

[0018] As a further improvement of the present invention, the electrochemical detection system includes a three-electrode system and a potential control module in contact with the three-electrode system. The three-electrode system is correspondingly arranged with multiple detection chambers. The three-electrode system includes a working electrode, a reference electrode, and a counter electrode.

[0019] As a further improvement of the present invention, the signal acquisition and amplification unit includes an optical signal acquisition unit, a photomultiplier tube, a signal amplification circuit and a noise filtering unit connected in sequence, and the control system includes a power management unit, a drive control unit, a data processing unit and a data output unit.

[0020] A method for rapid detection of viruses in indoor air, using the aforementioned rapid indoor air virus detection system, includes the following steps:

[0021] (1) Adjust the position of the trapping mesh system at the air outlet of the ventilation equipment so that the trapping mesh is aligned with the air outlet;

[0022] (2) After the ventilation equipment is turned on, the airflow passes through the collection mesh, and virus-carrying aerosol particles are intercepted by the collection mesh;

[0023] (3) After the set capture time is reached, the control system drives the membrane flipping component to flip the capture membrane at a certain angle through the membrane fixing component and dock it with the rinsing system. After docking, the capture membrane stops flipping.

[0024] (4) The flushing system is automatically started. The side of the trapping membrane that is rich in viruses is flushed with the flushing solution to wash away the viruses. The eluent containing the viruses is used as a virus sample and introduced into the inlet of the microfluidic system.

[0025] (5) The virus sample is delivered to multiple detection chambers via the microchannel assembly. The virus in the virus sample binds to the virus-specific recognition probe on the inner wall of the detection chamber. At the same time, the detection antibody in the detection chamber binds to another antigen on the surface of the virus to form a sandwich immune complex.

[0026] (6) The electrochemical detection system generates an electrochemiluminescence signal, the signal acquisition and amplification unit captures the electrochemiluminescence signal and converts it into an electrical signal, amplifies the electrical signal, and transmits it to the control system for data analysis;

[0027] (7) The control system calculates the virus concentration, determines whether the virus is negative or positive, and outputs the test results.

[0028] The beneficial effects of this invention are:

[0029] (1) The detection system is small in size and low in cost, and is suitable for ventilation systems such as air purifiers or air conditioners in homes, offices, shopping malls, hospital waiting areas, etc. It uses the airflow of existing equipment to achieve virus sampling, simplifies the detection process, and lowers the threshold for use.

[0030] (2) It has a strong ability to capture low-concentration viral aerosol particles and has high detection sensitivity.

[0031] (3) By combining the trapping membrane interception, microfluidic processing and electrochemiluminescence detection technologies, and signal amplification technology, we can achieve efficient capture of airborne viruses and rapid parallel detection of multiple targets, providing technical support for early monitoring and prevention of airborne viruses.

[0032] (4) It is portable and easy to use, simple to operate, and there will be no cross-contamination during sampling and testing.

[0033] (5) Routine collection, short detection time, and results within minutes, achieving high efficiency and speed in detection. Attached Figure Description

[0034] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0035] Figure 1 This is a schematic diagram of the initial state of the preferred embodiment of the present invention in conjunction with the ventilation equipment;

[0036] Figure 2 This is a schematic diagram of the structure of the trapping mesh after it has been flipped over and is connected to the rinsing system according to a preferred embodiment of the present invention;

[0037] Figure 3 This is a top view schematic diagram of the assembly of the microfluidic system and the electrochemical detection system of the present invention;

[0038] Figure 4 A schematic diagram of the structure of the detection system of the present invention with an outer casing;

[0039] Figure 5 This is a schematic diagram illustrating virus capture and detection.

[0040] Figure 6 for Schematic diagram of electrochemical reaction mechanism;

[0041] Figure 7 This is a block diagram showing the connection of the control system of the present invention to the trapping mesh system, the rinsing system, the microfluidic system, the electrochemical detection system, and the signal acquisition and amplification unit.

[0042] Figure 8 This is a structural block diagram of the signal acquisition and amplification unit of the present invention;

[0043] Figure 9 This is a flowchart of the detection method of the present invention;

[0044] In the diagram: 1. Capturing membrane system; 11. Capturing membrane; 111. Membrane block; 12. Membrane fixing assembly; 121. Detachable adapter plate; 13. Membrane flipping assembly; 131. Stepper motor; 132. Positioning sensor; 133. Drive shaft; 2. Rinsing system; 21. Rinsing chamber; 22. Spray assembly; 221. Moving mechanism; 222. Spray head; 23. Rinsing fluid storage tank; 24. Guide tube; 25. Rinsing pipe; 26. First peristaltic pump; 27. Second peristaltic pump; 3. Microfluidic system; 31. Microfluidic chip; 32. Inlet; 33. Detection chamber; 34. Microchannel assembly; 341. Branching microchannel. 35. Channel, Virus-specific recognition probe, 36. Buffer storage bottle, 37. Waste liquid chamber, 38. Buffer tubing, 39. Third peristaltic pump, 4. Electrochemical detection system, 41. Three-electrode system, 411. Working electrode, 412. Reference electrode, 413. Counter electrode, 42. Potential control module, 5. Signal acquisition and amplification unit, 51. Optical signal acquisition unit, 52. Photomultiplier tube, 53. Signal amplification circuit, 54. Noise filtering unit, 6. Control system, 61. Power management unit, 62. Drive control unit, 63. Data processing unit, 64. Data output unit, 7. Housing, 8. Ventilation equipment. Detailed Implementation

[0045] To enable those skilled in the art to better understand the technical solutions of this invention, the technical solutions of the embodiments of this invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this invention, and not all embodiments. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of this invention.

[0046] Please see Figure 1 , Figure 2 , Figure 3 , Figure 7This application discloses an indoor airborne virus rapid detection system, including a capture mesh system 1, a flushing system 2, a microfluidic system 3, an electrochemical detection system 4, a signal acquisition and amplification unit 5, and a control system 6. Each system and unit works collaboratively to complete virus capture, sample processing, diversion, detection, and signal analysis. The capture mesh system 1 includes a capture mesh 11, a mesh fixing component 12, and a mesh flipping component 13. The capture mesh 11 is disposed on the mesh fixing component 12, and the mesh flipping component 13 is connected to the mesh fixing component 12 and drives the mesh fixing component 12 to flip. The flushing system 2 is used to dock with the flipped mesh fixing component 12 and wash away the viruses captured by the capture mesh 11. The microfluidic system 3 includes a microfluidic chip 31, an inlet 32 ​​located on the microfluidic chip 31, multiple detection chambers 33, and a microchannel assembly 34 connecting the inlet 32 ​​and the multiple detection chambers 33. Each detection chamber 33 has a virus-specific recognition probe 35 fixed to its inner wall. The inlet 32 ​​is connected to the rinsing system 2. The electrochemical detection system 4 generates an electrochemiluminescence signal. The signal acquisition and amplification unit 5 captures the electrochemiluminescence signal, converts it into an electrical signal, and amplifies the electrical signal. The control system 6 is connected to the trapping membrane system 1, the rinsing system 2, the microfluidic system 3, the electrochemical detection system 4, and the signal acquisition and amplification unit 5.

[0047] In this embodiment, the capturing mesh 11 includes multiple mesh blocks 111, and the mesh fixing assembly 12 includes a detachable adapter plate 121 and multiple adjustable clips (not shown in the figure). The multiple mesh blocks 111 are mounted on the detachable adapter plate 121 via multiple adjustable clips. The adjustable clips can be easily removed from or attached to the detachable adapter plate 121, allowing for the removal and replacement of the mesh blocks 111 without tools. Preferably, the mesh blocks 111 are made of PP meltblown fabric, which can better adhere to and capture viruses. Preferably, the mesh blocks 111 have a size of 2cm × 2cm and a pore size of 1-5 micrometers. In this embodiment, 25 independent mesh blocks 111 are arranged in a 5×5 array. Both the detachable adapter plate 121 and the adjustable clips are injection molded from ABS plastic. The detachable adapter plate 121 has a grid structure, including multiple small grids, the number and size of which match the mesh blocks 111. In this embodiment, the detachable adapter plate 121 is a 3mm thick 5×5 plastic mesh. It is understood that the detachable adapter plate 121 can also be configured as a combination of an upper frame and a lower frame, with the capturing mesh 11 clamped between the upper and lower frames to fix the capturing mesh 11. The mesh flipping assembly 13 includes a stepper motor 131 and a positioning sensor 132. The output end of the stepper motor 131 is connected to a drive shaft 133, the mesh fixing assembly 12 is connected to the drive shaft 133, and the positioning sensor 132 is located in the rinsing system 2. When the stepper motor 131 operates, the drive shaft 133 rotates, causing the mesh fixing assembly 12 to rotate, thus flipping the capturing mesh 11. When it flips to align with the top of the rinsing system 2, the positioning sensor 132 triggers a signal, stopping the flipping.

[0048] Preferably, the rinsing system 2 includes a rinsing chamber 21, a spray assembly 22, a rinsing fluid storage tank 23, and a guide pipe 24. The spray assembly 22 includes a rotating telescopic arm 221 disposed at the top edge of the rinsing chamber 21 and a spray head 222 connected to the rotating telescopic arm 221. A rinsing pipe 25 connects the rinsing fluid storage tank 23 and the rinsing chamber 21, and a first micro peristaltic pump 26 is disposed on the rinsing pipe 25. The guide pipe 24 is connected to the bottom of the rinsing chamber 21 and the sample inlet 32, and a second micro peristaltic pump 27 is disposed on the guide pipe 24. A positioning sensor 132 is disposed on the inner side of the top of the rinsing chamber 21. The rinsing chamber 21 is made of transparent polypropylene (PP) material, and the inner wall of the rinsing chamber 21 is smooth to avoid sample residue. The top of the rinsing chamber 21 is open and sealed to the flipped detachable adapter plate 122. To improve sealing, a sealing strip can be provided on the inner side of the top of the rinsing chamber 21. Rotation and extension are achieved by rotating the telescopic arm 221, allowing the spray head 222 to move above any of the trapping mesh blocks 111. Preferably, the spray head 222 is a micro-spray head with an orifice diameter of 0.2 mm, and its coverage area matches that of a single trapping mesh block 111. The rinsing solution storage tank 23 stores PBS buffer containing 0.05% Tween-20, which effectively elutes viruses adhering to the trapping mesh 11 without destroying viral antigen activity. The guide tube 24 is made of polytetrafluoroethylene with an inner diameter of 1 mm, ensuring that the eluted virus-containing rinsing solution is introduced into the inlet 32 ​​of the microfluidic system 3.

[0049] The microfluidic system 3 is responsible for precisely diverting and delivering the virus-containing flushing solution to multiple detection chambers 33. The microfluidic chip 31, fabricated from PDMS material using soft photolithography, is 50-100 mm long. The number of detection chambers 33 corresponds to the number of branched microchannels in the microchannel assembly 34. The microchannel assembly 34 is bifurcated, facilitating the arrangement of multiple branched microchannels within the limited space of the microfluidic chip 31, enabling simultaneous multi-virus detection. Specifically, the microchannel assembly 34 includes eight branched microchannels 341. Preferably, the inner diameter of each branched microchannel 341 is 50-100 μm, and its surface is hydrophobically treated to ensure no liquid residue. In this embodiment, eight detection chambers 33 correspond one-to-one with eight branched microchannels 341. Each detection chamber 33 has a volume of 5-10 μL, and its inner wall is covalently fixed with virus-specific recognition probes 35. The virus-specific recognition probes 35 are monoclonal antibodies, nucleic acid aptamers, or antigen-binding fragments targeting different viruses. Different probes are fixed in different detection chambers 33. Figure 5 As shown, the virus is captured by the virus-specific recognition probe 35 of the detection chamber 33, forming a "fixed probe-virus" complex, which is then fixed to the inner wall surface of the detection chamber 33. Pre-labeled components are placed inside the detection chamber 33. The detection antibody, with an antibody concentration of 0.5-1 μg / mL, The conjugation ratio with the antibody is 1:5-1:10, and the co-reactant TPrA (tripropylamine) is included, labeled with... The detection antibody recognizes and pairs with the "fixed probe-virus" complex to form a "fixed probe-virus-detection antibody" sandwich immune complex, enabling parallel detection of multiple viruses. Figure 5 In the diagram, a represents the inner wall of the detection chamber, c represents the virus particles, d represents the detection antibody, and e represents... mark.

[0050] In this embodiment, the microfluidic system 3 further includes a buffer storage bottle 36 and multiple waste chambers 37. The buffer storage bottle 36 is connected to the guide tube 24 via a buffer tube 38. A third micro-peristaltic pump 39 is installed on the buffer tube 36. The waste chambers 37 are located within the microfluidic chip 31 and are connected to the detection chambers 33. The third micro-peristaltic pump 39 provides power for the liquid flow, controlling the flow rate to 10-20 μL / min to ensure that the sample or buffer solution is evenly distributed to each detection chamber 33 without retention or cross-contamination. The buffer storage bottle 36 is located below the microfluidic chip 31, has a volume of 5-10 mL, and contains PBS buffer solution. The buffer storage bottle 36 is connected to the guide tube 24 and stably delivers the buffer solution to each detection chamber 33 via the third micro-peristaltic pump 39. This buffer solution is used to clean the detection chambers 33, fill the detection chambers 33 with buffer solution, and discharge unbound impurities and antibodies to the waste chambers 37. The waste chambers 37 store excess waste solution overflowing from the detection chambers 33.

[0051] Electrochemical detection system 4 based on The electrochemiluminescence mechanism enables virus detection, including a three-electrode system 41 and a potential control module 42 in contact with the three-electrode system 41. The three-electrode system 41 is correspondingly set with multiple detection chambers 33. The three-electrode system 41 includes a working electrode 411, a reference electrode 412 and a counter electrode 413.

[0052] The three-electrode system 41 is an array structure, with the virus sample in each detection chamber 33 in contact with the three-electrode system 41. The working electrode 411 is a gold electrode or a glassy carbon electrode, the reference electrode 412 is an Ag / AgCl electrode, and the counter electrode 413 is a platinum wire electrode. The electrode spacing is 1-2 mm to ensure the stability of the electrochemical signal. The potential control module 42 provides a stable oxidation potential for the three-electrode system 41. Calibration is performed based on the stable potential of the reference electrode 412. The potential of the working electrode 411 is set to 1.1V-1.2V. This potential allows the "fixed probe-virus-detection antibody" sandwich immune complex to... Oxidized to And it causes the co-reactant TPRA to lose electrons to generate Free radicals are subsequently generated. , and The reaction is reduced to the excited state. excited state When it rapidly falls back to its ground state, it releases photons with wavelengths of 450-550 nm, generating an electrochemiluminescence signal, such as... Figure 6 As shown.

[0053] The signal acquisition and amplification unit 5 is used to detect, convert, and enhance signal strength, and filter noise interference. It includes an optical signal acquisition unit 51, a photomultiplier tube 52, a signal amplification circuit 53, and a noise filtering unit 54 connected in sequence. Figure 8 As shown.

[0054] The optical signal acquisition unit 51 employs a photodiode array, located below the microfluidic chip 31, and has acquisition channels corresponding to each detection chamber 33. The acquisition wavelength range is 400-600 nm, with a response time ≤1 ms. It captures electrochemiluminescence signals in real time and converts them into electrical signals, which are then transmitted to the photomultiplier tube 52. The photomultiplier tube 52 performs preliminary amplification of the electrical signals output from the optical signal acquisition unit 51, with an amplification factor of [missing value]. The signal amplification circuit 53 is an amplifier circuit composed of a two-stage operational amplifier, with an adjustable gain range of [number missing]. The signal amplification is adaptively adjusted according to signal strength, performing two-stage amplification to ensure that weak signals generated by low-concentration viruses can be identified. The noise filtering unit 54 uses a low-pass filter with a cutoff frequency of 50-100Hz to filter interference signals such as ambient light noise and circuit thermal noise, thereby improving the signal-to-noise ratio.

[0055] The control system 6 includes a power management unit 61, a drive control unit 62, a data processing unit 63, and a data output unit 64. The power management unit 61 provides stable 5V and 12V voltages, supporting external power supplies or a built-in lithium battery. The 5V voltage powers the drive control unit 62 and the signal acquisition and amplification unit 5, while the 12V voltage powers the stepper motor 131, the first micro peristaltic pump 26, the second micro peristaltic pump 27, and the third micro peristaltic pump 39. The drive control unit 62 includes a stepper motor drive circuit and a micro peristaltic pump drive circuit to start and stop the stepper motor 131, the first micro peristaltic pump 26, the second micro peristaltic pump 27, and the third micro peristaltic pump 39. The data processing unit 63 uses an STM32 series microcontroller. It sends a "start potential output" command to the potential control module 42 via a communication interface such as I2C / SPI. Based on preset detection parameters, such as a target potential of 1.15V, it outputs a corresponding digital signal. The high-precision digital-to-analog converter (DAC) within the potential control module 42 converts the digital signal into an analog voltage, which is output through the contact electrode. The reference electrode 412 monitors the actual potential of the working electrode 411 in real time and outputs a feedback signal, which is transmitted to the analog signal input port of the data processing unit 63. Using built-in algorithms such as PID control, it compares the "target potential" with the "actual potential fed back by the reference electrode," calculates the deviation, and adjusts the digital signal output to the DAC based on the deviation. The DAC synchronously corrects the analog voltage output. Simultaneously, the data processing unit 63 performs analog-to-digital conversion on the amplified electrical signal and calculates the virus concentration using preset algorithms such as threshold comparison and standard curve methods to determine the presence of the target virus. The data output unit 64 supports Bluetooth or Wi-Fi connectivity, allowing real-time transmission of detection results, such as virus type, concentration, and detection time, to mobile terminals such as smartphones and tablets, providing a clear and intuitive display of the detection report for easy information retrieval.

[0056] The preferred flushing system 2, microfluidic system 3, electrochemical detection system 4, signal acquisition and amplification unit 5, and control system 6 are all housed within a single housing 7, such as... Figure 4 As shown, this enables the integration of various systems and units, while also facilitating the movement of the detection system.

[0057] Please see Figure 9 This application also discloses a rapid indoor air virus detection method using the aforementioned rapid indoor air virus detection system, comprising the following steps:

[0058] S1: Adjust the position of the trapping mesh system 1 at the air outlet of the ventilation equipment 8 so that the trapping mesh 11 is aligned with the air outlet;

[0059] S2: After the ventilation equipment 8 is turned on, the airflow passes through the collection mesh 11, and virus-carrying aerosol particles are intercepted by the collection mesh 11.

[0060] S3: After the set capture time is reached, the control system 6 drives the membrane flipping assembly 13 to flip the capture membrane 11 at a certain angle through the membrane fixing assembly 12 and dock it with the rinsing system 2. After docking, the capture membrane 11 stops flipping.

[0061] S4: Automatically start the rinsing system 2, rinse the virus-rich side of the trapping mesh 11 with rinsing fluid, elute the virus, and introduce the virus-containing eluent as a virus sample into the inlet 32 ​​of the microfluidic system 3.

[0062] S5: The virus sample is delivered to multiple detection chambers 33 via the microchannel component 34. The virus in the virus sample binds to the virus-specific recognition probe 35 on the inner wall of the detection chamber 33. At the same time, the detection antibody in the detection chamber 33 binds to another antigen on the surface of the virus to form a sandwich immune complex.

[0063] S6: The electrochemical detection system 4 generates an electrochemiluminescence signal, the signal acquisition and amplification unit 5 captures the electrochemiluminescence signal and converts it into an electrical signal, amplifies the electrical signal, and transmits it to the control system 6 for data analysis;

[0064] S7: Control system 6 calculates virus concentration, determines whether the virus is negative or positive, and outputs the test results.

[0065] To better illustrate the rapid indoor air virus detection method of the present invention, the following are the specific steps.

[0066] 1. System Installation and Preparation: Secure the collecting mesh 11 to the detachable adapter plate 121 using adjustable clips. Adjust the detachable adapter plate 121 to the air purifier's outlet position. Remove or install the individual mesh blocks 111 on the detachable adapter plate 121 according to the outlet size to ensure the overall size of the collecting mesh 11 matches the outlet. Adjust the angle of the detachable adapter plate 121 using the mesh flipping assembly 13 to ensure the collecting mesh 11 is perpendicular to the airflow direction. Check the detection antibodies installed in the detection chamber 33 of the microfluidic system 3 to ensure sufficient antibody levels. Power on all systems and units and perform self-tests. It is understood that the ventilation equipment 8 is not limited to an air purifier; it can also be an air conditioner. In the initial state, the collecting mesh 11 of the detection system can be positioned facing the air conditioner's outdoor unit outlet using a support assembly.

[0067] 2. Virus capture: After the air purifier is turned on, the airflow passes through the capture mesh 11. Virus-carrying aerosol particles smaller than 5 micrometers are intercepted by the capture mesh 11 and adhere to the capture mesh 11. The capture time is set to 30-120 minutes, which can be adjusted according to the size of the indoor space or the monitoring frequency.

[0068] 3. Flipping and docking: After the set capture time is reached, the control system 6 starts the stepper motor 131 through the drive control unit 62, drives the mesh fixing assembly 12 to flip, flips the capture mesh 11 to a certain angle, and seals and docks it with the rinsing chamber 21 of the rinsing system 2. The positioning sensor 132 controls the flipping to stop.

[0069] 4. Sample elution and introduction: The rinsing system 2 is automatically started, the first peristaltic pump 26 works, the rinsing solution is evenly sprayed through the spray head 222 onto the side of the trapping mesh 11 that is rich in viruses, the virus is eluted into the rinsing chamber 21, and the elution solution is introduced into the sample inlet 32 ​​of the microfluidic system 3 through the guide tube 24.

[0070] 5. Sample and buffer delivery: The second peristaltic pump 27 is activated, delivering the virus sample in the eluent through 8 branched microchannels 341 to 8 detection chambers 33, respectively corresponding to the detection of influenza A virus H1N1, influenza A virus H3N2, influenza B virus, novel coronavirus SARS-CoV-2, respiratory syncytial virus RSV, human rhinovirus HRV, adenovirus AdV, and human parainfluenza virus type 3 HPIV-3. The virus in the sample binds to the virus-specific recognition probe 35 on the inner wall of the detection chamber 33, and the detection antibody simultaneously binds to another antigen on the surface of the virus to form a sandwich immune complex. Subsequently, the buffer in the buffer storage bottle 36 is delivered to the detection chamber 33 through the third peristaltic pump 39, and the waste liquid overflows into the waste liquid chamber 37.

[0071] 6. Electrochemical detection and signal processing: The potential control module 42 applies an oxidation potential of 1.15V to the three-electrode system 41 to excite... The light is oxidized, generating an electrochemiluminescence signal. The light signal acquisition unit 51 captures the signal and converts it into an electrical signal. After initial amplification by the photomultiplier tube 52, secondary amplification by the signal amplification circuit 53, and filtering by the noise filtering unit 54, the signal is transmitted to the control system 6 for data analysis.

[0072] 7. Result Output: The data processing unit 63 of the control system 6 calculates the virus concentration through an algorithm, determines whether the virus is negative or positive, and transmits the test results, such as "negative for influenza A virus H1N1, negative for influenza A virus H3N2, negative for influenza B virus, positive for novel coronavirus SARS-CoV-2, negative for respiratory syncytial virus RSV, negative for human rhinovirus HRV, negative for adenovirus AdV, and negative for human parainfluenza virus type 3 HPIV-3", to a mobile terminal, such as a user's mobile phone or tablet with a matching APP, and provides the user with an early warning notification. The user can check the test results at any time.

[0073] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0074] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A rapid indoor air virus detection system, characterized in that, include: A net film catching system includes a net film catching device, a net film fixing component, and a net film flipping component. The net film catching device is disposed on the net film fixing component, and the net film flipping component is connected to the net film fixing component and drives the net film fixing component to flip. A flushing system is used to dock with the flipped mesh fixing assembly and to wash away the viruses captured by the trapping mesh; A microfluidic system, comprising a microfluidic chip, an inlet disposed on the microfluidic chip, multiple detection chambers, and a microchannel assembly connecting the inlet and the multiple detection chambers, wherein a virus-specific recognition probe is fixed on the inner wall of each detection chamber, and the inlet is connected to the flushing system; Electrochemical detection system, used to generate electrochemiluminescence signals; The signal acquisition and amplification unit is used to capture the electrochemiluminescence signal and convert it into an electrical signal, and amplify the electrical signal; The control system is connected to the trapping mesh system, the rinsing system, the microfluidic system, the electrochemical detection system, and the signal acquisition and amplification unit, respectively.

2. The indoor air virus rapid detection system according to claim 1, characterized in that, The capturing mesh includes multiple mesh blocks, and the mesh fixing assembly includes a detachable adapter plate and multiple adjustable buckles. The multiple mesh blocks are set on the detachable adapter plate by the multiple adjustable buckles.

3. The indoor air virus rapid detection system according to claim 2, characterized in that, The mesh block is made of PP meltblown fabric.

4. The indoor air virus rapid detection system according to claim 2 or 3, characterized in that, The mesh block has a size of 2cm × 2cm and a pore size of 1-5 micrometers.

5. The indoor air virus rapid detection system according to claim 1, characterized in that, The membrane flipping assembly includes a stepper motor and a positioning sensor. The output end of the stepper motor is connected to a drive shaft. The membrane fixing assembly is connected to the drive shaft. The positioning sensor is located in the rinsing system.

6. The indoor air virus rapid detection system according to claim 1, characterized in that, The rinsing system includes a rinsing chamber, a spray assembly, a rinsing fluid storage tank, and a guide pipe. The spray assembly includes a rotating telescopic arm disposed at the top edge of the rinsing chamber and a spray head connected to the rotating telescopic arm. A rinsing pipe is connected between the rinsing fluid storage tank and the rinsing chamber. A first micro peristaltic pump is disposed on the rinsing pipe. The guide pipe is connected to the bottom of the rinsing chamber and the sample inlet, respectively. A second micro peristaltic pump is disposed on the guide pipe.

7. The indoor air virus rapid detection system according to claim 1, characterized in that, The microfluidic system also includes a buffer storage bottle and multiple waste liquid chambers. The buffer storage bottle is connected to the guide tube through a buffer tube. A third micro peristaltic pump is installed on the buffer tube. The waste liquid chambers are located inside the microfluidic chip and are connected to the detection chamber.

8. The indoor air virus rapid detection system according to claim 1, characterized in that, The electrochemical detection system includes a three-electrode system and a potential control module in contact with the three-electrode system. The three-electrode system is correspondingly arranged with multiple detection chambers. The three-electrode system includes a working electrode, a reference electrode, and a counter electrode.

9. The indoor air virus rapid detection system according to claim 1, characterized in that, The signal acquisition and amplification unit includes an optical signal acquisition unit, a photomultiplier tube, a signal amplification circuit, and a noise filtering unit connected in sequence. The control system includes a power management unit, a drive control unit, a data processing unit, and a data output unit.

10. A rapid detection method for indoor airborne viruses, characterized in that, Using the indoor air virus rapid detection system as described in any one of claims 1-9, the following steps are included: (1) Adjust the position of the trapping mesh system at the air outlet of the ventilation equipment so that the trapping mesh is aligned with the air outlet; (2) After the ventilation equipment is turned on, the airflow passes through the collection mesh, and virus-carrying aerosol particles are intercepted by the collection mesh; (3) After the set capture time is reached, the control system drives the membrane flipping component to flip the capture membrane at a certain angle through the membrane fixing component and dock it with the rinsing system. After docking, the capture membrane stops flipping. (4) The flushing system is automatically started. The side of the trapping membrane that is rich in viruses is flushed with the flushing solution to wash away the viruses. The eluent containing the viruses is used as a virus sample and introduced into the sample inlet of the microfluidic system. (5) The virus sample is delivered to multiple detection chambers via the microchannel assembly. The virus in the virus sample binds to the virus-specific recognition probe on the inner wall of the detection chamber. At the same time, the detection antibody in the detection chamber binds to another antigen on the surface of the virus to form a sandwich immune complex. (6) The electrochemical detection system generates an electrochemiluminescence signal, the signal acquisition and amplification unit captures the electrochemiluminescence signal and converts it into an electrical signal, amplifies the electrical signal, and transmits it to the control system for data analysis; (7) The control system calculates the virus concentration, determines whether the virus is negative or positive, and outputs the test results.

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