Cell culture fluid and processing method thereof

Abstract

The invention relates to the field of cell culture fluids, in particular to a cell culture fluid and a processing method thereof, and the processing method comprises the following steps: S1, rapidly preparing triple distilled water through a distillation device; s2, after the three-distilled water is cooled, adding the mesenchymal stem cell cold freeze-dried powder; s3, after the dry powder is completely dissolved, adjusting the pH value; s4, adding an antibiotic solution; and S5, sterilizing the filter membrane, and packaging. The distillation unit comprises a liquid containing box, a heating device, a side pipe, an upper cover, a communicating mechanism and a cooling mechanism, the heating device is arranged at the lower end of the liquid containing box, the side pipe is obliquely arranged on the side face of the liquid containing box, the communicating mechanism is connected to the upper end of the side pipe, the upper cover is fixedly installed at the upper end of the liquid containing box, and one end of the cooling mechanism is connected to the upper cover; the other end of the cooling mechanism is connected with the communicating mechanism. The three-distilled water is quickly obtained by using the distillation device, so that the processing efficiency of the cell culture fluid is improved.

Description

Technical Field

[0001] This invention relates to the field of cell culture media, and in particular to a cell culture media and its processing method. Background Technology

[0002] After decades of development, cell culture technology has become a mature technology for producing many important proteins. In the cell culture process, the cell culture medium is an indispensable substance. During the preparation of the cell culture medium, sterile water is required, generally triple-distilled water. Currently, most methods for obtaining distilled water involve adding a certain amount of purified water to a distillation apparatus, heating and distilling it until the purified water is completely converted to distilled water, and then adding the distilled water back into the distillation apparatus, repeating this process to obtain triple-distilled water. This repetitive operation greatly reduces the efficiency of obtaining triple-distilled water. Summary of the Invention

[0003] The purpose of this invention is to provide a cell culture medium and its processing method, which rapidly obtains triple-distilled water by using a distillation apparatus, thereby improving the processing efficiency of the cell culture medium.

[0004] The objective of this invention is achieved through the following technical solution:

[0005] A method for processing cell culture medium includes the following steps:

[0006] S1. Rapidly prepare triple-distilled water using a distillation apparatus;

[0007] S2. After the triple-distilled water has cooled, add the mesenchymal stem cell freeze-dried powder.

[0008] S3. After the dry powder has completely dissolved, adjust the pH value.

[0009] S4. Add antibiotic solution;

[0010] S5. After sterilizing the filter membrane, seal it.

[0011] In step S2, dry powder is added slowly.

[0012] In step S2, the dry powder is added while stirring.

[0013] In step S3, the pH value is adjusted to 7.1-7.2.

[0014] When adjusting the pH value, add sodium bicarbonate solution.

[0015] HEPES solution is also used when adjusting the pH value.

[0016] The antibiotic solution is a solution of penicillin and streptomycin.

[0017] The distillation apparatus includes multiple distillation units that can be connected end-to-end to form continuous distillation.

[0018] The distillation unit includes a liquid tank, a heating device, a side tube, a top cover, a connecting mechanism, and a cooling mechanism. The heating device is located at the lower end of the liquid tank, the side tube is inclinedly located on the side of the liquid tank, the connecting mechanism is connected to the upper end of the side tube, the top cover is fixedly installed at the upper end of the liquid tank, one end of the cooling mechanism is connected to the top cover, and the other end of the cooling mechanism is connected to the connecting mechanism.

[0019] The cell culture medium processed by the aforementioned cell culture medium processing method comprises 1L of triple-distilled water, 20g of mesenchymal stem cell lyophilized powder, and 200U / mL of antibiotic solution. Attached Figure Description

[0020] Figure 1 This is a flowchart illustrating the cell culture medium processing method. Figure 1 ;

[0021] Figure 2 This is a flowchart illustrating the cell culture medium processing method. Figure 2 ;

[0022] Figure 3 This is a schematic diagram of the distillation apparatus;

[0023] Figure 4 This is a schematic diagram of the distillation unit;

[0024] Figure 5 This is a cross-sectional view of the liquid tank.

[0025] Figure 6 This is a schematic diagram of the structure of a connecting pipe;

[0026] Figure 7 This is a schematic diagram of the cooling mechanism;

[0027] Figure 8 This is a cross-sectional view of the cooling mechanism.

[0028] Figure 9 This is a structural diagram of the base plate.

[0029] In the picture:

[0030] Liquid container 101; heating device 102; side pipe 103; connecting box 104; ring plate 105; inner rod 106;

[0031] 201 connecting pipe; 202 connecting hole; 203 limiting bracket; 204 spring;

[0032] Top cover 301; Bend 302; Thin-walled tube 303; Serpentine tube 304; Adjusting screw 305;

[0033] Base plate 401; support leg tube 402; threaded shaft 403; adjusting shaft 404. Detailed Implementation

[0034] like Figure 1-2 The cell culture medium processing method is described in detail below:

[0035] A method for processing cell culture medium includes the following steps:

[0036] S1. Rapidly prepare triple-distilled water using a distillation apparatus;

[0037] S2. After the triple-distilled water has cooled, add the mesenchymal stem cell freeze-dried powder.

[0038] S3. After the dry powder has completely dissolved, adjust the pH value.

[0039] S4. Add antibiotic solution;

[0040] S5. After sterilizing the filter membrane, seal it.

[0041] In step S2, dry powder is added slowly.

[0042] In step S2, the dry powder is added while stirring.

[0043] In step S3, the pH value is adjusted to 7.1-7.2.

[0044] When adjusting the pH value, add sodium bicarbonate solution.

[0045] HEPES solution is also used when adjusting the pH value.

[0046] The antibiotic solution is a solution of penicillin and streptomycin.

[0047] like Figure 3-9 The distillation apparatus is described in detail below:

[0048] The distillation apparatus includes multiple distillation units that can be connected end-to-end to form continuous distillation.

[0049] The distillation unit includes a liquid tank 101, a heating device 102, a side tube 103, a top cover 301, a connecting mechanism, and a cooling mechanism. The heating device 102 is located at the lower end of the liquid tank 101, the side tube 103 is inclinedly located on the side of the liquid tank 101, the connecting mechanism is connected to the upper end of the side tube 103, the top cover 301 is fixedly installed at the upper end of the liquid tank 101, one end of the cooling mechanism is connected to the top cover 301, and the other end of the cooling mechanism is connected to the connecting mechanism.

[0050] During distillation, pure water is added to the liquid tank 101 of the first distillation unit, and the heating device 102 is activated. The water vapor generated after the water in the liquid tank 101 evaporates will enter the cooling mechanism through the top cover 301 and be cooled into distilled water in the cooling mechanism. Then, it flows along the cooling mechanism into the connecting mechanism of the second distillation unit, and then flows into the liquid tank 101 of the second distillation unit through the side pipe 103. After the liquid tank 101 of the second distillation unit stores an appropriate amount of distilled water, the heating device 102 of the second distillation unit can be activated to make the second distillation unit repeat the steps of the first distillation unit, so that the distilled water enters the third distillation unit, and so on, forming continuous distillation to obtain distilled water of different times. At the same time, the efficiency of obtaining distilled water is improved, and multiple distilled water can be obtained quickly.

[0051] like Figure 3-9 As shown:

[0052] The cooling mechanism includes a bent pipe 302, a thin-walled pipe 303, and a serpentine pipe 304. One end of the bent pipe 302 is slidably connected to the upper cover 301, the thin-walled pipe 303 slides inside the other end of the bent pipe 302, and the serpentine pipe 304 is disposed through the thin-walled pipe 303.

[0053] Water vapor drifts through the top cover 301 into the bent tube 302, and then into the thin-walled tube 303. The water vapor liquefies in the bent tube 302 and the thin-walled tube 303. Because the wall of the thin-walled tube 303 is thin, it is easily affected by the outside world and maintains a low temperature. As a result, the water vapor is more likely to liquefy in the thin-walled tube 303. At the same time, because the thin-walled tube 303 is inclined, the distillation flow flows down along the thin-walled tube 303 until it flows into the connecting mechanism.

[0054] The serpentine tube 304 is used to introduce cooling liquid, so that water vapor can be liquefied more quickly when it comes into contact with the thin-walled tube 303 and the serpentine tube 304, thereby improving the water vapor liquefaction efficiency.

[0055] Further:

[0056] The cooling mechanism also includes an adjusting screw 305, which rotates on the thin-walled tube 303 and is threadedly connected to the bent tube 302.

[0057] By rotating the adjusting screw 305, the overall length of the thin-walled tube 303 and the bend 302 can be adjusted, thereby increasing the path of water vapor flow in the thin-walled tube 303 and the bend 302. Thus, the overall length of the thin-walled tube 303 and the bend 302 can be adjusted according to the ambient temperature of the device, ensuring the maximum efficiency of distilling water.

[0058] like Figure 3-9 As shown:

[0059] The communication mechanism includes a communication box 104, a ring plate 105, a communication pipe 201, a communication hole 202, a limiting frame 203, and a spring 204. The communication box 104 is fixedly connected to the upper end of the side pipe 103, the ring plate 105 is fixedly connected to the upper end of the communication box 104, the communication pipe 201 slides inside the ring plate 105, the communication hole 202 is provided on the side of the communication pipe 201, the limiting frame 203 is fixedly connected to the communication pipe 201 and is limited and slidably connected to the outside of the communication box 104, and the spring 204 is provided between the limiting frame 203 and the communication box 104.

[0060] When the upper end of the connecting pipe 201 is not connected to the thin-walled pipe 303, that is, when the connecting pipe 201 is not under force and slides down in the ring plate 105, the connecting hole 202 on the connecting pipe 201 is located in the ring plate 105, so that the connecting pipe 201 and the connecting box 104 are not connected, thereby preventing water vapor from drifting out from the side pipe 103.

[0061] When the thin-walled tube 303 is connected to the upper end of the connecting tube 201, the thin-walled tube 303 will press down on the connecting tube 201, causing the connecting tube 201 to overcome the elastic force of the spring 204 and drive the connecting hole 202 to slide into the connecting box 104, so that the connecting tube 201 is connected to the connecting box 104, thereby allowing distilled water to flow from the thin-walled tube 303 into the connecting tube 201, then into the connecting box 104, and then into the liquid holding tank 101 through the side tube 103.

[0062] like Figure 3-9 As shown:

[0063] The distillation unit also includes an inner rod 106, a support tube 402, a base plate 401, a threaded shaft 403, and an adjusting shaft 404; multiple inner rods 106 are fixed to the lower end of the heating device 102, multiple support tubes 402 are respectively sleeved on multiple inner rods 106, the base plate 401 is fixed on multiple support tubes 402, the threaded shaft 403 rotates on the heating device 102 and is threadedly connected to the base plate 401, and the adjusting shaft 404 rotates at the lower end of the heating device 102 and is drively connected to the threaded shaft 403.

[0064] By rotating the adjusting shaft 404, the threaded shaft 403 can be driven to drive the base plate 401 through the threaded transmission, thereby causing the base plate 401 to drive the support tube 402 to slide on the inner rod 106, thus forming the support tube 402 to contact the ground to support the distillation unit. At the same time, the height of the unit can be adjusted so that the distillation device can adapt to different ground environments. In addition, the connection between two adjacent distillation units can be adapted to different lengths of the bent tube 302 and the thin-walled tube 303 by adjusting the height difference between the two liquid tanks 101.

[0065] Further:

[0066] The cell culture medium processed by the aforementioned cell culture medium processing method comprises 1L of triple-distilled water, 20g of mesenchymal stem cell lyophilized powder, and 200U / mL of antibiotic solution.

Claims

1. A method for processing a cell culture fluid, the method comprising: Includes the following steps: ​ S1. Rapidly prepare triple-distilled water using a distillation apparatus; S2. After the triple-distilled water has cooled, add the mesenchymal stem cell freeze-dried powder. S3. After the dry powder has completely dissolved, adjust the pH value. S4. Add antibiotic solution; S5. After sterilizing the filter membrane, seal it.

2. The cell culture fluid processing method of claim 1, wherein: In step S2, dry powder is added slowly.

3. The cell culture fluid processing method of claim 2, wherein: In step S2, the dry powder is added while stirring.

4. The cell culture fluid processing method of claim 1, wherein: In step S3, the pH value is adjusted to 7.1-7.

2.

5. The cell culture fluid processing method of claim 4, wherein: When adjusting the pH value, add sodium bicarbonate solution.

6. The cell culture fluid processing method of claim 5, wherein: HEPES solution is also used when adjusting the pH value.

7. The cell culture fluid processing method of claim 1, wherein: The antibiotic solution is a solution of penicillin and streptomycin.

8. The cell culture fluid processing method of claim 1, wherein: The distillation apparatus includes multiple distillation units that can be connected end-to-end to form continuous distillation.

9. The cell culture fluid processing method of claim 8, wherein: The distillation unit includes a liquid tank (101), a heating device (102), a side tube (103), a top cover (301), a connecting mechanism, and a cooling mechanism. The heating device (102) is located at the lower end of the liquid tank (101), the side tube (103) is inclinedly located on the side of the liquid tank (101), the connecting mechanism is connected to the upper end of the side tube (103), the top cover (301) is fixedly installed at the upper end of the liquid tank (101), one end of the cooling mechanism is connected to the top cover (301), and the other end of the cooling mechanism is connected to the connecting mechanism.

10. The cell culture fluid processed by the method of claim 1, wherein: The cell culture medium includes 1L of triple-distilled water, 20g of mesenchymal stem cell lyophilized powder, and 200U / mL of antibiotic solution.

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