Multifunctional visual sampling device for white and green tissues of hydrangea serrata leaves

Abstract

The invention relates to the technical field of hydrangea hemsleyana leaf sampling, in particular to a hydrangea hemsleyana leaf white and green tissue multifunctional visual sampling device which comprises a machine body, an objective lens module capable of moving front and back is arranged on the back of the machine body, and a sampling module used for separating and sampling leaves is installed on the objective lens module. The sampling module comprises a sampling unit and an adjusting unit, the sampling unit comprises a cutter capable of being in contact with a blade and cutting the blade open and an extractor for sampling the blade, and the extractor can spray out low-temperature gas and freeze and fix a sample. According to the method, the taken-out sample is quickly frozen, so that the problem that RNA (Ribonucleic Acid) is degraded due to the fact that the sample cannot be frozen in time after being taken out is solved.

Description

Technical Field

[0001] This invention relates to the field of sampling technology for silver-edged hydrangea leaves, specifically a multifunctional visual sampling device for the white-green tissue of silver-edged hydrangea leaves. Background Technology

[0002] As a typical variegated foliage plant, the green part of the leaves of the silver-edged hydrangea is rich in chlorophyll, while the white edge lacks functional chloroplasts. This natural chimeric characteristic of white and green tissue makes it an ideal material for studying the regulation of chloroplast development, differences in photosynthetic physiology, and gene expression regulation mechanisms in plants. In related molecular biology and plant physiology experiments, samples of both white edge tissue and green central tissue need to be obtained from the same leaf.

[0003] Current manual sampling methods mostly involve direct cutting with a scalpel or tearing with tweezers. Operators need to visually locate the boundary between the white and green tissues. Because the white-green transition zone of silver-edged hydrangea leaves is narrow and the boundary of some areas is blurred, sampling errors are very likely to occur, resulting in green tissue mixed into the white tissue sample or vice versa, causing sample contamination. Furthermore, the sample cannot be frozen in time when it is taken out, which can easily cause RNA degradation and affect the accuracy of subsequent transcriptome (RNA-seq) and qRT-PCR data. Summary of the Invention

[0004] The purpose of this invention is to provide a multifunctional visual sampling device for the white-green tissue of silver-edged hydrangea leaves, which solves the problem of RNA degradation caused by the inability to freeze the sample in time after it is taken out by rapidly freezing the sample.

[0005] To address the problems of existing technologies, this invention provides a multifunctional visual sampling device for the white-green tissue of silver-edged hydrangea leaves, comprising a main body. An objective lens module capable of moving back and forth is located on the back of the main body. A sampling module for separating and sampling the leaves is mounted on the objective lens module. The sampling module includes a sampling unit and an adjustment unit. The sampling unit includes a cutter capable of contacting and cutting the leaves, and an aspirator for sampling the leaves. The aspirator can spray low-temperature gas to freeze and fix the sample.

[0006] Preferably, the sampling unit includes a movable arm that can approach or move away from the blade. A rotating disk is rotatably mounted at the end of the movable arm, and the rotating disk is fixed to the cutter by a clamp. The position of the rotating disk away from the cutter is fixed to the suction device. When the rotating disk rotates, it can drive the cutter and the suction device to switch for cutting or sampling the blade.

[0007] Preferably, the sampling unit includes a suction head disposed at the end of the suction device, and the end of the suction device is also circumferentially and uniformly provided with nozzles for spraying out freezing gas.

[0008] Preferably, the sampling unit further includes a rotary drive component installed on the side of the moving arm away from the rotating disk. A rotary sealing joint is installed on the outer shell of the rotary drive component. One end of the rotary sealing joint is connected to a T-shaped pipe. The rotary shaft of the rotary drive component is connected to the rotating disk, and the inside of the rotary shaft is hollow. The center of the rotating disk has a first flow channel communicating with the rotary shaft. The first flow channel is connected to the suction head, and the rotary sealing joint is connected to the rotary shaft.

[0009] Preferably, the rotating disk has a second flow channel that communicates with the nozzle, the moving arm has a pipe interface, and the rotating disk has a connecting hole that communicates with the second flow channel.

[0010] Preferably, the machine body includes a base, a microscope eyepiece is installed at the front of the base, a gas tank is in the base, and the gas outlet of the gas tank is connected to a pipeline interface through a hose. The gas tank has a first solenoid valve for controlling the gas flow.

[0011] Preferably, the base has a negative pressure pump and a positive pressure pump inside, which are connected to a three-way pipe through pipes. The negative pressure pump and the positive pressure pump are respectively equipped with a second solenoid valve and a third solenoid valve to control the gas flow.

[0012] Preferably, the objective lens module includes a movable column that can move back and forth on the back of the body, and the front of the movable column has a first moving mechanism, on which an electron microscope objective lens that can move up and down is disposed.

[0013] Preferably, the adjustment unit includes a mounting bracket installed on the objective lens module, and the mounting bracket has support members on both sides. The support members have sliding grooves along their length. A support block is slidably arranged on the support member. The support block is fixed to the support member by bolts. A first moving module is vertically arranged on the support block. A second moving module that can move up and down is arranged on the first moving module. A moving block is slidably arranged on the second moving module. One end of the moving block is connected to a support platform. A rotating gimbal is rotatably arranged at the bottom of the support platform. A third moving module that can drive the sampling unit to move back and forth is connected to the rotating gimbal.

[0014] Preferably, the machine body also has a platform assembly, which includes a movable platform that can move left and right, and a plurality of reserved holes are evenly provided on the movable platform. The platform assembly also includes a second moving mechanism disposed in the machine body for driving the movable platform to move.

[0015] The advantages of this invention compared to the prior art are:

[0016] 1. This application utilizes a sampling unit as the core operating module to achieve leaf tissue sampling. The sampling unit is equipped with a rotatable disc, on which a cutter can be detachably mounted via a clamping structure. During operation, the sampling unit's displacement brings the cutter into contact with the target area of ​​the leaf, completing the slicing action and separating the chlorophyll and leucopteric tissues. After slicing, the aspirator in the sampling unit moves to the sampling area to contact the sample, activating the aspiration function to pick up the sample. During this process, a gas tank simultaneously delivers freezing gas to the nozzle. The directional spray of freezing gas from the nozzle rapidly freezes and solidifies the picked-up sample, effectively inhibiting RNase activity within the sample and preventing RNA degradation due to untimely freezing, thus ensuring the molecular biological activity of the sample.

[0017] 2. To address the challenges of efficient separation and rapid collection of frozen samples from the aspirator, this application further optimizes the fluid control structure of the sampling unit by employing a three-way valve, a negative pressure pump, and a positive pressure pump to construct a collaboratively controlled fluid loop. During sample aspiration, the negative pressure pump provides negative pressure suction to the aspirator head via the three-way valve, achieving stable sample adsorption. When the sampling unit moves the frozen sample into the pre-cooling container, the positive pressure pump activates and delivers positive pressure gas to the aspirator head via the three-way valve. This positive pressure propels the frozen sample rapidly from the aspirator head, allowing it to fall into the pre-cooling container. This design eliminates the need for additional auxiliary tools for sample transfer, effectively avoiding potential secondary contamination and tissue damage during the transfer process, while significantly improving sample collection efficiency. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the first three-dimensional structure of a multifunctional visual sampling device for the white-green tissue of silver-edged hydrangea leaves according to the present invention.

[0019] Figure 2 This is a schematic diagram of the second three-dimensional structure of a multifunctional visual sampling device for the white-green tissue of silver-edged hydrangea leaves according to the present invention.

[0020] Figure 3 This is a three-dimensional structural diagram of the body, objective lens module, and stage assembly of a multifunctional visual sampling device for white-green tissue of silver-edged hydrangea leaves according to the present invention.

[0021] Figure 4 This is a side view structural schematic diagram of a multifunctional visual sampling device for the white-green tissue of silver-edged hydrangea leaves according to the present invention.

[0022] Figure 5 This is a schematic diagram of the first three-dimensional structure of the sampling module of a multifunctional visual sampling device for white and green tissue of silver-edged hydrangea leaves according to the present invention.

[0023] Figure 6This is a schematic diagram of the second three-dimensional structure of the sampling module of a multifunctional visual sampling device for white-green tissue of silver-edged hydrangea leaves according to the present invention.

[0024] Figure 7 This is a three-dimensional structural diagram of the sampling unit of a multifunctional visual sampling device for white-green tissue of silver-edged hydrangea leaves according to the present invention.

[0025] Figure 8 This is a three-dimensional structural diagram of the rotating disk, cutter, and aspirator of a multifunctional visual sampling device for the white-green tissue of silver-edged hydrangea leaves according to the present invention.

[0026] Figure 9 This is a top view schematic diagram of a multifunctional visual sampling device for the white-green tissue of silver-edged hydrangea leaves according to the present invention.

[0027] Figure 10 This is a schematic diagram of the back view of a multifunctional visual sampling device for the white-green tissue of silver-edged hydrangea leaves according to the present invention.

[0028] The diagram is labeled as follows: 1. Main body; 11. Base; 12. Microscope eyepiece; 2. Objective lens module; 21. Moving column; 22. First moving mechanism; 23. Electron microscope objective lens; 3. Stage assembly; 31. Moving stage; 311. Pre-drilled hole; 32. Second moving mechanism; 4. Sampling module; 41. Mounting bracket; 42. Support component; 421. Slide groove; 43. Support block; 431. First moving module; 44. Second moving module; 441. Moving block; 442. Support platform; 443. Rotating gimbal; 45. Third moving module; 46. Moving arm; 461. Rotary disk; 462. Cutter; 463. Suction device; 4631. Suction head; 4632. Spray nozzle; 464. Rotation drive component; 465. T-connector; 466. Pipe interface; 5. Gas tank; 6. Negative pressure pump; 7. Positive pressure pump. Detailed Implementation

[0029] To further understand the features, technical means, and specific objectives and functions achieved by the present invention, the present invention will be described in further detail below with reference to the accompanying drawings and specific embodiments.

[0030] Reference Figures 1 to 8As shown, this invention provides a multifunctional visual sampling device for the white-green tissue of silver-edged hydrangea leaves, comprising a main body 1. An objective lens module 2 capable of moving back and forth is mounted on the back of the main body 1. A sampling module 4 for separating and sampling the leaves is mounted on the objective lens module 2. The main body 1 serves as the foundation and core component of the entire sampling device, primarily providing a stable mounting reference and support platform for other functional modules (objective lens module 2, sampling module 4, etc.). The main body 1 can integrate core components such as a power supply module and a control module (e.g., drive circuit, signal processing unit), providing power support and command transmission for the back-and-forth movement of the objective lens module 2 and the sampling action of the sampling module 4, ensuring the coordinated operation of all components of the entire device. The core function of the objective lens module 2 is to achieve visual observation and precise positioning of the sampling process, overcoming the drawbacks of manual sampling relying on visual positioning. It features a forward and backward movement function, allowing for clear focusing by adjusting the distance to the leaf sample. This facilitates clear observation of the boundary of the white-green tissue in the silver-edged hydrangea leaf by the operator, enabling precise locking of the target sampling area. The sampling module 4 includes a sampling unit and an adjustment unit. The adjustment unit primarily adjusts the spatial orientation and position parameters of the sampling unit. The sampling unit includes a cutter 462 that contacts and cuts the leaf, and an aspirator 463 that samples the leaf. The aspirator 463 can spray low-temperature gas to freeze and fix the sample. The cutter 462 primarily contacts the target area of ​​the leaf and performs a cutting action, achieving precise separation of the white-green tissue. The cutter 462 can be a scribing needle, a micro-cutting knife, or any device capable of cutting the leaf. The cutter 462, in conjunction with the positioning function of the adjustment unit and the objective lens module 2, precisely cuts along the visually locked white-green boundary, avoiding positioning deviations caused by manual operation. The aspirator 463's core function is to pick up and freeze-fix the target sample after cutting, solving the problems of contamination and damage during sample transfer after manual sampling. It possesses dual functions: firstly, a sampling function, which can stably pick up the diced target sample through negative pressure adsorption and other methods; secondly, a cryo-fixation function, which can rapidly lower the sample temperature by spraying low-temperature gas (such as liquid nitrogen) through an internal channel, quickly fixing the sample tissue cells, inhibiting RNase activity, and preventing RNA degradation due to untimely subsequent processing, thus ensuring the molecular biological activity of the sample and providing high-quality samples for subsequent experiments. The aspirator 463 can also be used in other forms, such as electric tweezers, miniature grippers, etc., as long as it has a gas spraying function.

[0031] In operation, the silver-edged hydrangea leaf to be sampled is fixed at the designated position. The operator starts the device via the control module of the main unit 1 (existing technology) and adjusts the objective lens module 2 to move back and forth until the boundary of the white-green tissue of the leaf is clearly observed through the objective lens module 2, thus locking the target sampling area. The cutting depth of the cutter 462 and the position of the aspirator 463 are adjusted via the adjustment unit of the sampling module 4 to ensure that the cutter 462 is aligned with the white-green tissue boundary and that the aspirator 463 is in a position that allows for rapid docking with the cut sample. The sampling module 4 moves, bringing the cutter 462 into contact with the target area of ​​the leaf, and precisely cuts along the locked white-green boundary, achieving separation of the target tissue (white edge or green central tissue) from the rest of the leaf. After cutting, the adjustment unit drives the aspirator 463 to move above the separated target sample, activating the aspiration function to stably adsorb the sample; simultaneously, the aspirator 463 sprays low-temperature gas to rapidly freeze and fix the sample, inhibiting RNA degradation. After cryo-fixation, the sampling unit includes a movable arm 46 that can approach or move away from the blade. A rotating disk 461 is rotatably mounted at the end of the movable arm 46, and the rotating disk 461 is fixed to a cutter 462 by a clamp. The position of the rotating disk 461 away from the cutter 462 is fixed to a suction device 463. When the rotating disk 461 rotates, it can drive the cutter 462 and suction device 463 to switch between cutting or sampling the blade. The suction device 463 carrying the sample is transferred to a pre-cooled sample storage container, the sample is released, and the entire sampling process is completed.

[0032] The sampling unit includes a movable arm 46 that can move closer to or further away from the blade. The movable arm 46 serves as the displacement drive component of the sampling unit, its core function being to move the rotating disk 461, cutter 462, and suction device 463 closer to or further away from the blade. Its displacement accuracy directly determines the docking accuracy between the sampling unit and the target area. Smooth movement can be achieved through electric drive, ensuring that the cutter 462 reaches the boundary of the white-green tissue and that the suction device 463 is aligned with the cut target sample. A rotating disk 461 is rotatably mounted at the end of the movable arm 46, and is fixed to the cutter 462 by a clamp. The position of the rotating disk 461 away from the cutter 462 is fixed to the suction device 463. When the rotating disk 461 rotates, it can drive the cutter 462 and suction device 463 to switch between cutting or sampling the blade. The rotating disk 461 is a rotatable structure and is the core component for switching between the functions of the cutter 462 and the suction device 463. The rotating motion can drive the cutter 462 and the extractor 463 to switch alternately to the working position, and the process of "cutting and sampling" can be completed without disassembling or replacing components, which greatly improves sampling efficiency.

[0033] The sampling unit includes a suction head 4631 located at the end of the suction device 463, and the end of the suction device 463 is also circumferentially and uniformly provided with nozzles 4632 for spraying freezing gas. The rotating disk 461 has a second flow channel communicating with the nozzles 4632. The moving arm 46 has a pipe interface 466, and the rotating disk 461 has a connecting hole communicating with the second flow channel. The body 1 includes a base 11, which contains a gas tank 5. The gas tank 5 is a storage component for freezing gas, providing a power source for the freezing function of the sampling unit. The gas tank 5 is filled with a low-temperature gas (such as liquid nitrogen) suitable for freezing plant tissue, and the outlet of the gas tank 5 is connected to the pipe interface 466 via a hose. The gas tank 5 has a first solenoid valve for controlling the gas flow. The first solenoid valve is the core control component for controlling the flow of freezing gas, and through linkage with the control module (existing technology) of the device, the timing control of the freezing gas injection is achieved. During the sampling process, after the suction head 4631 completes sample adsorption, the control module triggers the opening of the first solenoid valve, and the refrigeration gas in the gas tank 5 is delivered to the nozzle 4632 through the hose, pipe interface 466, connecting hole, and second flow channel to achieve sample freezing; after the sample freezing is completed, the first solenoid valve closes and stops gas injection. In order to prevent cold gas from leaking from the gap between the rotating disk 461 and the moving arm 46, a sealed bearing can be used for sealing.

[0034] After sample adsorption is complete, the control module triggers the opening of the first solenoid valve on the gas tank 5. The freezing gas in the gas tank 5 is delivered to the pipe interface 466 of the moving arm 46 through the hose, enters the second flow channel through the connecting hole on the rotating disk 461, and is finally directionally sprayed onto the sample adsorbed by the aspirator head 4631 through the circumferentially evenly distributed nozzles 4632 at the end of the aspirator 463. This achieves all-round uniform and rapid freezing of the sample, inhibits RNase activity, and avoids RNA degradation.

[0035] The sampling unit also includes a rotary drive 464 installed on the side of the moving arm 46 away from the rotating disk 461. A rotary sealing joint is mounted on the outer shell of the rotary drive 464, and one end of the rotary sealing joint is connected to a three-way pipe 465. The rotation shaft of the rotary drive 464 is connected to the rotating disk 461, and the inside of the rotation shaft is hollow. The center of the rotating disk 461 has a first flow channel communicating with the rotation shaft, which is connected to the suction head 4631. The rotary sealing joint is connected to the rotation shaft. The base 11 contains a negative pressure pump 6 and a positive pressure pump 7, which are connected to the three-way pipe 465 via pipes. The negative pressure pump 6 and the positive pressure pump 7 each have a second solenoid valve and a third solenoid valve for controlling the gas flow.

[0036] After the blade is cut, the rotary drive 464 drives the rotating disk 461 to rotate, switching the suction device 463 above the cut target sample. The moving arm 46 fine-tunes the distance between the suction device 463 and the sample to ensure precise alignment between the suction head 4631 and the sample. The control module triggers the second solenoid valve to open, and the negative pressure pump 6 starts to generate negative pressure. The negative pressure is transmitted through the pipeline to the three-way pipe 465, enters the hollow rotating shaft of the rotary drive 464 through the rotary sealing joint, and is then transmitted to the suction head 4631 through the first flow channel in the center of the rotating disk 461. The suction head 4631 generates negative pressure suction to stably adsorb the target sample. After adsorption, the sample is rapidly frozen and fixed. The moving arm 46 moves the aspirator 463 (carrying the frozen sample) into the pre-cooled sample container. The control module triggers the second solenoid valve to close and the third solenoid valve to open. The positive pressure pump 7 starts to generate positive pressure. The positive pressure is transmitted to the aspirator head 4631 through the pipe, tee 465, rotary sealing joint, hollow rotating shaft, and first flow channel. The positive pressure thrusts the sample to be quickly separated from the aspirator head 4631, and the sample falls accurately into the pre-cooled container, completing the entire sampling process. The negative pressure pump 6 and the positive pressure pump 7 can be of other forms; any device capable of generating positive and negative pressure is applicable.

[0037] The objective module 2 includes a movable column 21 that can reciprocate back and forth on the back of the body 1. The body 1 has a moving structure inside that drives the movable column 21. This moving structure can be driven by a motor and lead screw, or other methods. The front of the movable column 21 has a first moving mechanism 22, which can also be driven by a motor and lead screw, or other methods. An electron microscope objective 23 that can move up and down is mounted on the first moving mechanism 22. A microscope eyepiece 12 is also mounted on the front of the base 11.

[0038] After the operator places the silver-edged hydrangea leaf sample to be sampled at the preset sampling position, the moving column 21 moves back and forth on the back of the machine body 1 to initially adjust the distance between the electron microscope objective 23 and the leaf sample. Simultaneously, the first moving mechanism 22 moves the electron microscope objective 23 up and down to adjust its height, ensuring it is roughly aligned with the target observation area of ​​the leaf sample. The operator observes the leaf image captured by the electron microscope objective 23 through the microscope eyepiece 12 and sends adjustment commands to the control module based on the image clarity. The control module, according to the commands, drives the moving column 21 and the first moving mechanism 22 to perform fine-tuning adjustments until the electron microscope objective 23 achieves precise focus. The operator can then clearly observe the white-green tissue boundary of the silver-edged hydrangea leaf through the microscope eyepiece 12, especially the narrow, blurred transition area.

[0039] The adjustment unit includes a mounting bracket 41 mounted on the objective lens module 2, and the mounting bracket 41 has support members 42 on both sides. The support members 42 have a sliding groove 421 along their length. A support block 43 is slidably mounted on the support member 42. The support block 43 is fixed to the support member 42 by bolts. A first moving module 431 is vertically mounted on the support block 43. A second moving module 44 that can move up and down is mounted on the first moving module 431. A moving block 441 is slidably mounted on the second moving module 44. One end of the moving block 441 is connected to a support platform 442. A rotating gimbal 443 is rotatably mounted on the bottom of the support platform 442. A third moving module 45 that can drive the sampling unit to move back and forth is connected to the rotating gimbal 443.

[0040] Based on the lateral position of the silver-edged hydrangea leaf sample, the operator loosens the fixing bolts on the support block 43, pushes the support block 43 to move, and adjusts it to a suitable lateral position. Then, the bolts are tightened to fix the support block 43, completing the lateral coarse adjustment of the adjustment unit and the sampling unit. The first moving module 431 is activated, driving the second moving module 44 and all subsequent components to move vertically. Based on the thickness of the leaf sample and the vertical height of the target sampling area, the second moving module 44 is activated, driving the moving block 441 and all subsequent components to slide along the length of the second moving module 44. Based on the longitudinal position of the leaf sample, after adjusting to a suitable longitudinal position, the electric control rotating gimbal 443 is used to rotate horizontally, adjusting the working angle of the third moving module 45 and the sampling unit to ensure that the cutter 462 and the absorber 463 can dock with the target area in the best posture. The third moving module 45 is activated, driving the sampling unit to perform reciprocating fine movement, moving the cutter 462 closer to the leaf sample to complete the cutting action, or moving the absorber 463 closer to the cut target sample to complete the adsorption action. The first moving module 431, the second moving module 44, and the third moving module 45 can all adopt a motor-driven screw structure to achieve position adjustment of the sampling unit. This collaborative adjustment logic fundamentally solves the technical defects of manual sampling, which relies on visual positioning and lacks a precise adjustment structure. Through a multi-dimensional, hierarchical adjustment structure, it achieves precise positioning and flexible posture adjustment of the sampling unit, effectively avoiding sampling deviation and improving the accuracy, stability, and reliability of sampling operations.

[0041] The body 1 also has a platform assembly 3, which includes a movable platform 31 that moves left and right. The movable platform 31 has several pre-drilled holes 311 evenly distributed on it. The pre-drilled holes 311 are mainly used to install a fixing structure to fix the blade. The fixing structure can be a glass plate. The glass plate has a plug-in post that can be inserted into the pre-drilled hole 311. The blade is fixed by the glass plate to prevent the blade from moving when cutting. The platform assembly 3 also includes a second moving mechanism 32 disposed in the body 1 to drive the movable platform 31 to move.

[0042] The operator places the silver-edged hydrangea leaf to be sampled onto the movable stage 31 and secures the leaf edge using a fixing structure. This structure consists of a glass plate and a connecting post. The connecting post inserts into pre-drilled holes 311, while the glass plate directly fixes the leaf. Its core function is to ensure the silver-edged hydrangea leaf is flat and stably fixed, preventing displacement during sampling. The glass plate is made of transparent material with good light transmittance, ensuring it does not obstruct the imaging of the electron microscope objective 23 of the objective lens module 2. The precise fit between the connecting post and the pre-drilled holes 311 allows for quick installation and removal of the fixing structure, facilitating leaf placement and removal and improving operational efficiency. The second moving mechanism 32 moves the movable stage 31 left and right, precisely adjusting the lateral position of the leaf and placing the target sampling area within the observation field of objective lens module 2 and the working range of sampling module 4. The second moving mechanism 32 can be driven by a motor and lead screw, or other mechanisms capable of moving the movable stage 31.

[0043] The above embodiments only illustrate one or more implementations of the present invention, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of the present invention should be determined by the appended claims.

Claims

1. A multifunctional visual sampling device for the white-green tissue of silver-edged hydrangea leaves, characterized in that: The device includes a body (1), on the back of which is a lens module (2) that can move back and forth. The lens module (2) is equipped with a sampling module (4) for separating and sampling the leaf. The sampling module (4) includes a sampling unit and an adjustment unit. The sampling unit includes a cutter (462) that can contact the leaf and cut it open, and an aspirator (463) that can sample the leaf. The aspirator (463) can spray low-temperature gas and freeze and fix the sample.

2. The silver-edged, white-green tissue multifunctional visualized sampling device for leaves of a blade according to claim 1, characterized in that: The sampling unit includes a movable arm (46) that can approach or move away from the blade. A rotating disk (461) is rotatably mounted at the end of the movable arm (46), and the rotating disk (461) is fixed to the cutter (462) by a clamp. The position of the rotating disk (461) away from the cutter (462) is fixed to the suction device (463). When the rotating disk (461) rotates, it can drive the cutter (462) and the suction device (463) to switch for cutting or sampling the blade.

3. The silver-edged, white-green tissue multifunctional visualized sampling device of claim 2, wherein: The sampling unit includes a suction head (4631) disposed at the end of the suction device (463), and the end of the suction device (463) is also circumferentially and uniformly provided with a nozzle (4632) for spraying out freezing gas.

4. The multifunctional visual sampling device for the white-green tissue of silver-edged hydrangea leaves according to claim 3, characterized in that: The sampling unit also includes a rotary drive (464) installed on the side of the moving arm (46) away from the rotating disk (461). A rotary sealing joint is installed on the outer shell of the rotary drive (464). One end of the rotary sealing joint is connected to a three-way pipe (465). The rotating shaft of the rotary drive (464) is connected to the rotating disk (461), and the inside of the rotating shaft is hollow. The center of the rotating disk (461) has a first flow channel communicating with the rotating shaft. The first flow channel is connected to the suction head (4631), and the rotary sealing joint is connected to the rotating shaft.

5. A multifunctional visual sampling device for the white-green tissue of silver-edged hydrangea leaves according to claim 4, characterized in that: The rotating disk (461) has a second flow channel that communicates with the nozzle (4632). The moving arm (46) has a pipe interface (466), and the rotating disk (461) has a connecting hole that communicates with the second flow channel.

6. A multifunctional visual sampling device for the white-green tissue of silver-edged hydrangea leaves according to claim 5, characterized in that: The body (1) includes a base (11), and a microscope eyepiece (12) is installed at the front of the base (11). The base (11) has a gas tank (5), and the gas outlet of the gas tank (5) is connected to a pipe interface (466) through a hose. The gas tank (5) has a first solenoid valve for controlling the gas flow.

7. A multifunctional visual sampling device for the white-green tissue of silver-edged hydrangea leaves according to claim 6, characterized in that: The base (11) has a negative pressure pump (6) and a positive pressure pump (7) inside. The negative pressure pump (6) and the positive pressure pump (7) are connected to a three-way pipe (465) through pipes respectively. The negative pressure pump (6) and the positive pressure pump (7) have a second solenoid valve and a third solenoid valve for controlling the gas flow.

8. A multifunctional visual sampling device for the white-green tissue of silver-edged hydrangea leaves according to claim 1, characterized in that: The objective module (2) includes a movable column (21) that can move back and forth on the back of the body (1), and the front part of the movable column (21) has a first moving mechanism (22), on which an electron microscope objective (23) that can move up and down is provided.

9. A multifunctional visual sampling device for the white-green tissue of silver-edged hydrangea leaves according to claim 1, characterized in that: The adjustment unit includes a mounting bracket (41) mounted on the objective lens module (2), and the mounting bracket (41) has support members (42) on both sides. The support members (42) have a sliding groove (421) along their length direction. A support block (43) is slidably arranged on the support member (42). The support block (43) is fixed to the support member (42) by bolts. A first moving module (431) is vertically arranged on the support block (43). A second moving module (44) that can move up and down is arranged on the first moving module (431). A moving block (441) is slidably arranged on the second moving module (44). One end of the moving block (441) is connected to a support platform (442). A rotating gimbal (443) is rotatably arranged at the bottom of the support platform (442). A third moving module (45) that can drive the sampling unit to move back and forth is connected to the rotating gimbal (443).

10. A multifunctional visual sampling device for the white-green tissue of silver-edged hydrangea leaves according to claim 1, characterized in that: The body (1) also has a platform assembly (3), which includes a movable platform (31) that moves left and right, and a number of reserved holes (311) are evenly provided on the movable platform (31). The platform assembly (3) also includes a second moving mechanism (32) provided in the body (1) for driving the movable platform (31) to move.

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