A composition with anti-aging efficacy and its preparation method and application

Abstract

The application discloses a composition with anti-aging efficacy and a preparation method and application thereof, and belongs to the technical field of cosmetics. The composition is compounded by a traditional Chinese medicine active component, a moisturizing and penetration promoting component and a repair and anti-aging auxiliary component, wherein the traditional Chinese medicine active component is composed of three kinds of enzymolysis products and three kinds of fermentation extracts prepared respectively by differential complex enzymolysis and probiotic anaerobic fermentation process of six kinds of medicinal and edible traditional Chinese medicine raw materials. The application solves the pain points of low transdermal absorption rate, poor bioavailability and insufficient stability of the traditional Chinese medicine extract in the cosmetic system, realizes the small molecule of the active ingredient through process optimization, forms a multi-target point synergistic anti-aging system by matching the functional components, has excellent anti-aging repair efficacy, physical and chemical stability and skin safety, is suitable for various skin qualities, and can be widely applied to the preparation of anti-aging and repair cosmetics.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a composition with anti-aging effects, its preparation method, and its application. Background Technology

[0002] As we age, exposed to ultraviolet radiation, environmental pollution, and irregular lifestyles contribute to skin aging, including natural aging and photoaging. This manifests as collagen loss, elastin fiber breakage, and a decrease in hyaluronic acid levels, leading to wrinkles, sagging, dryness, and dullness. In recent years, active ingredients derived from natural plants have become a hot research topic in the anti-aging cosmetics field due to their high safety, gentle effects, and synergistic multi-target efficacy.

[0003] Cordyceps sinensis, ginseng, astragalus, rehmannia, angelica, and peony are all traditional medicinal and edible raw materials, rich in active ingredients such as polysaccharides, saponins, flavonoids, and alkaloids. They possess antioxidant, anti-inflammatory, and cell proliferation-promoting effects and have been widely used in cosmetics. However, current technologies for preparing extracts from these traditional Chinese medicine raw materials mostly employ traditional water extraction and alcohol precipitation processes, which present the following key challenges: First, the active ingredients in traditional water extracts are mostly large-molecule polysaccharides, proteins, and glycosides, with molecular weights generally greater than 10,000 Da. They have extremely poor penetration ability into the skin's stratum corneum barrier, low transdermal absorption rate, and insufficient bioavailability, resulting in actual anti-aging effects that are far lower than the results of in vitro experiments. Secondly, traditional water extracts have a wide molecular weight distribution range, and large molecules are prone to aggregation and flocculation in cosmetic systems, leading to problems such as product layering, precipitation, and discoloration. They also have poor storage stability, light stability, and cold and heat cycle stability, which seriously limits their high-dose addition and application in cosmetics. Third, existing technologies mostly use single enzymatic hydrolysis or single fermentation processes to process Chinese medicine raw materials. Although this can reduce the molecular weight to a certain extent, it does not differentiate the treatment based on the structural characteristics of the active ingredients of different Chinese medicine raw materials. It is impossible to simultaneously achieve efficient release of active ingredients, precise control of molecular weight and improvement of system stability, and it is also difficult to achieve synergistic anti-aging effects of multiple components.

[0004] Furthermore, existing traditional Chinese medicine anti-aging compositions often focus only on enhancing efficacy, neglecting the synergistic effect of combining active ingredients with moisturizing, penetration-enhancing, and repairing components in the cosmetic matrix. This fails to simultaneously achieve multiple effects of "deep anti-aging + barrier repair + system stability," making it difficult to meet consumers' high-quality demands for anti-aging cosmetics. Therefore, this paper proposes a composition with anti-aging effects, its preparation method, and its application. Summary of the Invention

[0005] The purpose of this invention is to overcome the above-mentioned defects of the prior art and provide a composition with anti-aging effects, its preparation method and application. By carrying out differentiated enzymatic hydrolysis and fermentation treatment on six kinds of Chinese herbal raw materials, and combining them with specific moisturizing and penetration-promoting components and repair and anti-aging auxiliary components, the core problems of low transdermal absorption rate, poor bioavailability and insufficient stability of cosmetic systems of traditional Chinese herbal extracts are solved. Multi-target synergistic anti-aging is achieved, while having excellent physicochemical stability and skin safety.

[0006] The objective of this invention can be achieved through the following technical solutions: To achieve the above-mentioned objectives, the present invention adopts the following technical solution: A composition with anti-aging effects, comprising, by weight, 10-50 parts of active Chinese herbal medicine components, 5-20 parts of moisturizing and penetration-promoting components, 0.5-10 parts of repairing and anti-aging auxiliary components, with the remainder being purified water, and a total weight of 100 parts.

[0007] The active components of the traditional Chinese medicine are composed of enzymatic hydrolysates of three kinds of traditional Chinese medicine raw materials and fermented extracts of three kinds of traditional Chinese medicine raw materials in a mass ratio of 1:1; the three kinds of traditional Chinese medicine raw materials used for enzymatic hydrolysis are Cordyceps sinensis, ginseng root, and peony root, and the three kinds of traditional Chinese medicine raw materials used for fermentation are Astragalus membranaceus root, Rehmannia glutinosa root, and Angelica sinensis root.

[0008] The moisturizing and penetration-enhancing components, by weight, include 3-15 parts of butylene glycol and 2-5 parts of panthenol.

[0009] The repair and anti-aging auxiliary components, by weight, include 0.3-6 parts of Centella asiatica root extract and 0.2-4 parts of ectoine.

[0010] Furthermore, the complex enzyme in the enzymatic hydrolysate is composed of cellulase, pectinase, and β-glucanase in a mass ratio of 2:1:1, and the amount of complex enzyme added is 0.2%-0.5% of the mass of the enzymatic hydrolysate suspension.

[0011] Furthermore, the complex microbial strain of the fermented extract is composed of Lactobacillus plantarum, Bifidobacterium, and Lactobacillus acidophilus in a mass ratio of 1:1:1, and the inoculation amount is 2%-4% of the mass of the fermentation medium.

[0012] The preparation method of the above-mentioned composition with anti-aging effects includes the following steps: S1. Preparation of enzymatic hydrolysate (1) Pretreatment: Cordyceps sinensis, ginseng root and peony root are washed and dried at 40℃, pulverized and passed through an 80-mesh sieve. They are weighed accurately at a mass ratio of 1:2:1, mixed evenly and obtained enzymatic hydrolysis raw material powder. (2) Preparation of the solution: Add the enzymatic hydrolysis raw material powder and purified water into the enzymatic hydrolysis tank at a mass ratio of 1:15, stir at 30 r / min for 30 min until completely dispersed, and obtain the raw material suspension; (3) Compound enzymatic hydrolysis: The pH of the raw material suspension was adjusted to 5.0-5.5 using citrate-sodium citrate buffer, the compound enzyme was added, and after stirring evenly, the temperature was raised to 45-50℃ and enzymatic hydrolysis was carried out in a sealed environment at a constant temperature for 2-4 hours, with low stirring at 15r / min during the process. (4) Enzyme inactivation and purification: After the enzymatic hydrolysis is completed, the enzyme hydrolysate is heated to 100℃ and kept in a boiling water bath for 10 min to inactivate the enzyme. It is then rapidly cooled to 25℃ and centrifuged at 4000 r / min for 15 min. The supernatant is then filtered through a 0.22 μm microporous membrane to remove bacteria, and the hydrolysate is sealed and stored at 4℃ for later use. S2. Preparation of Fermentation Extracts (1) Pretreatment: Wash the roots of Astragalus membranaceus, Rehmannia glutinosa, and Angelica sinensis, dry them at 40℃, pulverize them and pass them through an 80-mesh sieve. Weigh them accurately according to a mass ratio of 2:1:1, mix them evenly, and obtain fermentation raw material powder. (2) Culture medium preparation: Fermentation raw material powder and purified water were added to the fermenter at a mass ratio of 1:20, stirred at 30r / min for 30min until completely dispersed, sealed and autoclaved at 121℃ for 20min, and then naturally cooled to 37℃ to obtain the fermentation culture medium. (3) Activation of bacterial strains: Lactobacillus plantarum, Bifidobacterium, and Lactobacillus acidophilus were inoculated into sterile MRS liquid medium and activated in an anaerobic incubator at 37°C for 24 hours to obtain three activated bacterial strains, all with a viable count ≥1×10⁻⁶. 9 CFU / mL; (4) Anaerobic fermentation: Mix the three activated bacterial cultures in a mass ratio of 1:1:1 to obtain a compound bacterial culture, add 3% of the fermentation medium mass to the fermentation medium, stir at 15 r / min for 10 min to mix, and anaerobic ferment at 37℃ for 72 h. (5) Sterilization and purification: After fermentation, the fermentation liquid is heated to 100℃ and kept in a boiling water bath for 10 min for sterilization. It is then rapidly cooled to 25℃ and centrifuged at 4000r / min for 15 min. The supernatant is then filtered through a 0.22μm microporous membrane to remove bacteria, and the fermentation extract is obtained. It is then sealed and stored at 4℃ for later use. S3. Preparation of compounded compositions (1) Weigh out purified water, butanediol and panthenol according to the formula, add them to the emulsification tank, stir at 30 r / min, heat to 40℃, stir for 20 min until completely dissolved, and obtain the aqueous base solution. (2) After the aqueous base solution is cooled to 25°C, add the enzymatic hydrolysate and fermentation extract according to the formula amount, and stir at 30r / min for 30min until completely mixed. (3) Add Centella asiatica root extract and ectoine according to the formula amount, and continue stirring at 30 r / min for 20 min until completely dispersed and uniform. Filter through a 0.45 μm microporous membrane to obtain the composition with anti-aging effect.

[0013] The beneficial effects of this invention are: (1) This invention employs differentiated enzymatic hydrolysis and fermentation processes to address the structural characteristics of active ingredients in different Chinese herbal raw materials: Through compound enzymatic hydrolysis, macromolecular cordyceps polysaccharides, ginsenosides, and paeoniflorin in Cordyceps sinensis, ginseng root, and peony root are degraded into small-molecule oligosaccharides, oligopeptides, and secondary saponins with a molecular weight less than 2000 Da; through probiotic fermentation, astragalus polysaccharides, rehmannia oligosaccharides, ferulic acid, and other components in Astragalus root, Rehmannia root, and Angelica root are fully released and transformed to obtain small-molecule active substances with concentrated molecular weight distribution. When the enzymatic hydrolysate and fermented extract are combined, the active ingredients can quickly penetrate the stratum corneum of the skin, with a transdermal absorption rate more than 3 times higher than that of traditional water extracts. This effectively scavenges free radicals and significantly promotes the synthesis of type I collagen and elastin and the secretion of hyaluronic acid in human skin fibroblasts, fundamentally improving aging symptoms such as wrinkles, sagging, and dryness, thus achieving deep anti-aging.

[0014] (2) Through precise control of enzymatic hydrolysis and fermentation processes, this invention controls the molecular weight of active ingredients in traditional Chinese medicine within the range of 500-2000 Da, with a molecular weight distribution coefficient (PDI) close to 1, significantly reducing the risk of aggregation and flocculation of large-molecule polysaccharides and proteins. At the same time, the small-molecule active ingredients have excellent compatibility with butylene glycol, panthenol, Centella asiatica root extract, and ectoine, which can significantly improve the storage stability, light stability, thermal cycling stability, and centrifugal stability of the composition system. After 6 months of storage at room temperature, there is no stratification, precipitation, or discoloration, and the pH value change rate is ≤3%. This solves the industry pain points of traditional Chinese medicine extracts being prone to stratification, precipitation, and discoloration in cosmetics, and allows for high-dose addition without affecting the stability of the product system.

[0015] (3) This invention combines the active ingredients of traditional Chinese medicine (TCM) through enzymatic hydrolysate and fermentation extract, along with moisturizing and penetration-promoting components and repairing and anti-aging auxiliary components, to form a multi-target synergistic system: the active TCM components are responsible for scavenging free radicals and promoting collagen synthesis, thus achieving core anti-aging; butylene glycol and panthenol have both moisturizing and penetration-promoting effects, which can further enhance the transdermal efficiency of active ingredients, while replenishing skin moisture and repairing the stratum corneum; Centella asiatica root extract can promote skin barrier repair and reduce inflammatory reactions; ectoine can protect skin cells from damage caused by ultraviolet rays and external stimuli, and delay photoaging. After the five components are combined, a full-chain anti-aging effect of "antioxidant-collagen-strong moisturizing-barrier repair-anti-photoaging" is achieved, with efficacy far superior to compositions with single ingredients or single processes.

[0016] (4) The present invention adopts a green preparation process of biological enzymatic hydrolysis and probiotic fermentation, with no organic solvent residue. No preservatives, fragrances, pigments or other irritating ingredients are added in the entire preparation process. It has been verified by skin irritation test to be non-irritating and is suitable for various skin types such as dry, oily, combination and sensitive skin. It has extremely high cosmetic application value. Detailed Implementation

[0017] To further illustrate the technical means and effects adopted by the present invention to achieve its intended purpose, the following detailed description, in conjunction with embodiments, provides a detailed explanation of the specific implementation methods, structures, features, and effects of the present invention. All raw materials used in this embodiment are cosmetic-grade raw materials, all bacterial strains used are probiotic strains permitted for use in cosmetics, and all instruments used are standard testing instruments used in the cosmetics industry.

[0018] Example 1 This embodiment provides a composition with anti-aging effects, with a total mass of 100 parts by weight, and the formula is as follows: Thirty active components of traditional Chinese medicine: including 15 parts of enzymatic hydrolysate of Cordyceps sinensis-ginseng root-peony root and 15 parts of fermented extract of Astragalus membranaceus root-Rehmannia glutinosa root-Angelica sinensis root. 12 parts of moisturizing and penetration-enhancing components: 8 parts butylene glycol and 4 parts panthenol Repairing and anti-aging auxiliary components (5 parts): including 3 parts Centella asiatica root extract and 2 parts ectoine. 53 parts purified water The composition preparation method of this embodiment is as follows: Preparation of S1 enzyme hydrolysate (1) Pretreatment: Cordyceps sinensis, ginseng root and peony root are washed and dried at 40℃, pulverized and passed through an 80-mesh sieve. They are weighed accurately at a mass ratio of 1:2:1, mixed evenly and obtained enzymatic hydrolysis raw material powder. (2) Preparation of the solution: Add the enzymatic hydrolysis raw material powder and purified water into the enzymatic hydrolysis tank at a mass ratio of 1:15, stir at 30 r / min for 30 min until completely dispersed, and obtain the raw material suspension; (3) Compound enzymatic hydrolysis: The pH of the raw material suspension was adjusted to 5.2 using citrate-sodium citrate buffer. A compound enzyme was added, which consisted of cellulase, pectinase and β-glucanase in a mass ratio of 2:1:1. The total amount of compound enzyme added was 0.3% of the total mass of the raw material suspension. After stirring evenly, the temperature was raised to 48℃ and enzymatic hydrolysis was carried out in a sealed environment at a constant temperature for 3 hours, with low-speed stirring at 15r / min during the process. (4) Enzyme inactivation and purification: After the enzymatic hydrolysis is completed, the enzymatic hydrolysate is heated to 100℃ and kept in a boiling water bath for 10 min to complete the enzyme inactivation. Then, it is rapidly cooled to 25℃. The cooled enzymatic hydrolysate is transferred to a centrifuge and centrifuged at 4000 r / min for 15 min. The supernatant is taken and filtered through a 0.22 μm microporous membrane for sterilization to obtain the enzymatic hydrolysate. It is then sealed and stored at 4℃ for later use. Preparation of S2 fermentation extract (1) Pretreatment: Wash the roots of Astragalus membranaceus, Rehmannia glutinosa, and Angelica sinensis, dry them at 40℃, pulverize them and pass them through an 80-mesh sieve. Weigh them accurately according to a mass ratio of 2:1:1, mix them evenly, and obtain fermentation raw material powder. (2) Culture medium preparation: Fermentation raw material powder and purified water were added to the fermenter at a mass ratio of 1:20, stirred at 30 r / min for 30 min until completely dispersed, sealed and heated to 121℃, autoclaved for 20 min, and then naturally cooled to 37℃ to obtain fermentation culture medium; (3) Activation of bacterial strains: Lactobacillus plantarum, Bifidobacterium, and Lactobacillus acidophilus were inoculated into sterile MRS liquid culture medium and activated in an anaerobic incubator at 37℃ for 24 hours to obtain three activated bacterial strains. The viable count of the three activated bacterial strains was detected by plate count method, and the viable count of the three activated bacterial strains was ≥1×10⁻⁶. 9 CFU / mL; (4) Anaerobic fermentation: Mix the three activated bacterial cultures in a mass ratio of 1:1:1 to obtain a compound bacterial culture. Add the compound bacterial culture to the fermentation medium at an inoculation rate of 3% of the total mass of the fermentation medium. Stir at 15 r / min for 10 min until uniform. After sealing, anaerobic ferment at 37℃ for 72 h. (5) Sterilization and purification: After fermentation, the fermentation liquid is heated to 100°C and sterilized by keeping it in a boiling water bath for 10 minutes. Then it is rapidly cooled to 25°C. The cooled fermentation liquid is transferred to a centrifuge and centrifuged at 4000 r / min for 15 minutes. The supernatant is taken and filtered through a 0.22 μm microporous membrane to remove bacteria, and the fermentation extract is obtained. It is sealed and stored at 4°C for later use. Preparation of S3 composition (1) Accurately weigh purified water, butanediol and panthenol according to the formula, add them to the emulsification tank, stir at 30 r / min, heat to 40℃, stir for 20 min until completely dissolved, and obtain the aqueous base solution. (2) After the aqueous base solution is cooled to 25°C, add the enzymatic hydrolysate and fermentation extract according to the formula amount, stir at 30r / min, and stir for 30min until completely homogeneous. (3) Add Centella asiatica root extract and ectoine according to the formula amount, keep the stirring speed at 30 r / min, continue stirring for 20 min until completely dispersed and uniform, filter through a 0.45 μm microporous membrane to obtain the composition with anti-aging effect, the dosage form of which is an essence.

[0019] Example 2 This embodiment provides a composition with anti-aging effects, which differs from Example 1 only in the content of the formulation components. The total mass parts are 100 parts, and the formulation is as follows: Twenty active components from traditional Chinese medicine: including 10 parts of enzymatic hydrolysate of Cordyceps sinensis-ginseng root-peony root and 10 parts of fermented extract of Astragalus membranaceus root-Rehmannia glutinosa root-Angelica sinensis root. Moisturizing and penetration-enhancing components (8 parts): 5 parts butylene glycol and 3 parts panthenol Repairing and anti-aging auxiliary components (3 parts): Centella asiatica root extract (2 parts) and ectoine (1 part) 69 parts purified water The preparation method of the composition in this embodiment is completely consistent with that in Example 1.

[0020] Example 3 This embodiment provides a composition with anti-aging effects, which differs from Example 1 only in the content of the formulation components. The total mass parts are 100 parts, and the formulation is as follows: Forty active components of traditional Chinese medicine: including 20 parts of enzymatic hydrolysate of Cordyceps sinensis-ginseng root-peony root and 20 parts of fermented extract of Astragalus membranaceus root-Rehmannia glutinosa root-Angelica sinensis root. Moisturizing and penetration-enhancing components (15 parts): including 10 parts butylene glycol and 5 parts panthenol. Repairing and anti-aging auxiliary components (8 parts): including 5 parts Centella asiatica root extract and 3 parts ectoine. 37 parts purified water The preparation method of the composition in this embodiment is completely consistent with that in Example 1.

[0021] Comparative Example 1 The difference between this comparative example and Example 1 is that the extracts of the six Chinese herbal raw materials were prepared by the traditional water extraction and alcohol precipitation method, without enzymatic hydrolysis and fermentation. The remaining components, contents, and preparation steps were completely consistent with those of Example 1.

[0022] Traditional water extraction and alcohol precipitation process: Cordyceps sinensis, ginseng root, peony root, astragalus root, rehmannia root, and angelica root are pulverized and passed through an 80-mesh sieve. They are mixed according to the mass ratio in Example 1, and mixed with purified water at a ratio of 1:15. The mixture is extracted twice in a 90°C water bath for 2 hours each time. The extracts are combined, centrifuged at 4000 r / min for 15 min, and the supernatant is collected. The supernatant is concentrated to a relative density of 1.10, and ethanol is added until the alcohol precipitation concentration reaches 70%. The mixture is allowed to stand at 4°C for 24 hours. The supernatant is collected to recover the ethanol, and the mixture is filtered through a 0.22 μm microporous membrane to obtain the water extract of traditional Chinese medicine, which replaces the enzymatic hydrolysate and fermentation extract in Example 1.

[0023] Comparative Example 2 The difference between this comparative example and Example 1 is that all six kinds of Chinese herbal raw materials were treated with the enzymatic hydrolysis process of Example 1 without fermentation. The remaining components, contents, and preparation steps are completely consistent with those of Example 1.

[0024] Comparative Example 3 The difference between this comparative example and Example 1 is that all six kinds of Chinese herbal raw materials were processed using the fermentation process of Example 1 without enzymatic hydrolysis, while the remaining components, contents, and preparation steps were completely consistent with Example 1.

[0025] Comparative Example 4 The difference between this comparative example and Example 1 is that only two kinds of Chinese herbal raw materials (Cordyceps sinensis and ginseng root) are enzymatically hydrolyzed, while the remaining four kinds (peony root, astragalus root, rehmannia root, and angelica root) are fermented. The other components, contents, and preparation steps are completely consistent with Example 1.

[0026] Comparative Example 5 The difference between this comparative example and Example 1 is that: four kinds of Chinese herbal raw materials (Cordyceps sinensis, ginseng root, peony root, and astragalus root) were enzymatically hydrolyzed, and the remaining two kinds (Rehmannia root and Angelica root) were fermented. The other components, contents, and preparation steps were completely consistent with Example 1.

[0027] Comparative Example 6 The difference between this comparative example and Example 1 is that the Chinese herbal raw materials used for enzymatic hydrolysis are replaced with wolfberry root, Ganoderma lucidum, and licorice root, while the raw materials used for fermentation, the remaining components, their contents, and the preparation steps are completely consistent with Example 1.

[0028] Comparative Example 7 The difference between this comparative example and Example 1 is that butanediol is removed from the formula and replaced with an equal amount of purified water. The remaining components, contents, and preparation steps are completely consistent with Example 1.

[0029] Comparative Example 8 The difference between this comparative example and Example 1 is that panthenol is removed from the formulation and replaced with an equal amount of purified water, while the remaining components, contents, and preparation steps are completely consistent with Example 1.

[0030] Comparative Example 9 The difference between this comparative example and Example 1 is that the Centella Asiatica root extract was removed from the formula and replaced with an equal amount of purified water. The remaining components, contents, and preparation steps are completely consistent with Example 1.

[0031] Comparative Example 10 The difference between this comparative example and Example 1 is that ectoine is removed from the formula and replaced with an equal amount of purified water, while the remaining components, contents, and preparation steps are completely consistent with Example 1.

[0032] Comparative Example 11 The difference between this comparative example and Example 1 is that the mass ratio of enzymatic hydrolysate to fermentation extract is 3:1 (22.5 parts of enzymatic hydrolysate and 7.5 parts of fermentation extract), the total mass of active components of traditional Chinese medicine remains unchanged, and the remaining components, contents, and preparation steps are completely consistent with Example 1.

[0033] Comparative Example 12 The difference between this comparative example and Example 1 is that the mass ratio of the enzymatic hydrolysate to the fermentation extract is 1:3 (7.5 parts of enzymatic hydrolysate and 22.5 parts of fermentation extract), the total mass of the active components of traditional Chinese medicine remains unchanged, and the remaining components, contents, and preparation steps are completely consistent with Example 1.

[0034] Performance testing For the composition samples of the above 3 examples and 12 comparative examples, four categories of performance tests were conducted: molecular weight distribution, transdermal absorption rate, anti-aging efficacy, and physicochemical stability. All tests were conducted in 3 parallel experiments, and the average value of the results was taken. The specific test methods and results are as follows.

[0035] 1. Molecular weight distribution detection High-performance gel permeation chromatography (GPC) was used for detection. Chromatographic conditions: TSKgel G2000SWxl gel column (7.8 mm × 300 mm, 5 μm); mobile phase: 0.1 mol / L sodium sulfate aqueous solution (containing 0.05% sodium azide); flow rate: 1.0 mL / min; column temperature: 35℃; detection wavelength: 280 nm; injection volume: 20 μL. Standard curves were plotted using dextran standards with molecular weights of 10000, 5000, 2000, 1000, and 500 Da. The number-average molecular weight (Mn) and weight-average molecular weight (Mw) of the samples were determined, and the molecular weight distribution coefficient (PDI) was calculated (PDI = Mw / Mn; the closer the value is to 1, the more uniform the molecular weight distribution).

[0036] 2. In vitro transdermal absorption rate detection A modified Franz diffusion cell method was used, with SPF-grade Kunming mouse abdominal skin as the transdermal barrier. The effective permeability area was 2.8 cm², the receiving cell volume was 7 mL, and the receiving solution was physiological saline containing 20% ​​ethanol. The stirring speed was 300 r / min, and the water bath temperature was 32 ± 0.5℃. The skin was fixed in the diffusion cell with the stratum corneum facing the supply cell and the dermis facing the receiving cell. 1 mL of sample was added to the supply cell, and 0.5 mL samples were taken at 2 h, 4 h, 8 h, 12 h, and 24 h, and an equal volume of isothermal receiving solution was added. The total content of ginsenoside Rg1, paeoniflorin, and verbascoside in the receiving solution was determined by HPLC. The cumulative transdermal absorption rate over 24 h was calculated using the formula: Cumulative transdermal absorption rate (%) = (Total active ingredients in the receiving cell / Initial total active ingredients in the supply cell) × 100%.

[0037] 3. Free radical scavenging ability test (1) DPPH free radical scavenging rate: Take 2 mL of sample (diluted 10 times with anhydrous ethanol), add 2 mL of 0.2 mmol / L DPPH anhydrous ethanol solution, let stand at room temperature in the dark for 30 min, measure the absorbance at 517 nm, and calculate the scavenging rate; (2) ABTS free radical scavenging rate: Prepare ABTS working solution (absorbance at 734nm 0.70±0.02), take 0.2mL of sample (diluted 10 times with PBS) and add 3.8mL of ABTS working solution, let stand at room temperature in the dark for 6min, measure absorbance at 734nm and calculate scavenging rate; (3) Hydroxyl radical scavenging rate: The reaction system was prepared by o-phenanthroline-Fe²+ oxidation method, kept in a water bath at 37℃ for 60 min, and the absorbance was measured at 536 nm to calculate the scavenging rate.

[0038] 4. Cellular level anti-aging efficacy detection Using human skin fibroblasts HFF-1, the non-cytotoxic concentration of the sample was first determined by the CCK-8 assay. The cells were then cultured at a concentration of 1×10⁻⁶. 5 Cells were seeded per well in 6-well plates and cultured for 24 hours. Then, culture medium containing 10% non-cytotoxic sample was added. The blank control group was given an equal volume of culture medium without the sample. After 48 hours of culture, the cell supernatant was collected. The contents of type I collagen, elastin, and hyaluronic acid were detected using human COL-I, ELN, and HAELISA kits, respectively. The secretion rate / synthesis rate was calculated using the formula: secretion rate (%) = (content of sample group / content of blank control group - 1) × 100%.

[0039] 5. Physicochemical stability testing (1) Stability of room temperature storage: The sample was sealed in a transparent vial and stored at 25°C and 60% relative humidity for 6 months. The appearance, color, layering / precipitation were observed at 0, 1, 3 and 6 months. The pH value was measured and the absorbance was measured at 450 nm. The pH value change rate and absorbance change rate were calculated. (2) Light stability: The sample was sealed in a transparent vial and continuously irradiated at 25°C for 30 days in a 4500±500lx UV light box. The changes in appearance and color were observed, and the rate of change of absorbance at 450nm was detected. (3) Stability of thermal cycling: The sample was placed in a thermal cycling test chamber. One cycle was frozen at -15℃ for 12 hours and baked at 45℃ for 12 hours. Ten consecutive cycles were performed to observe whether stratification, precipitation, or flocculation occurred and to detect the pH change rate. 6. Skin irritation test Referring to the rabbit skin irritation / corrosion test method in the "Cosmetic Safety Technical Specifications", three healthy New Zealand white rabbits were selected. 0.5 mL of the sample was applied to the intact skin after hair removal and left on for 4 hours. Skin erythema and edema were observed at 1 hour, 24 hours, 48 ​​hours and 72 hours, and scores were calculated to evaluate irritation.

[0040] Table 1. Molecular weight distribution detection results of each embodiment and comparative sample. Example 1 1268 1421 1.12 Example 2 1305 1474 1.13 Example 3 1243 1398 1.12 Comparative Example 1 12856 28543 2.22 Comparative Example 2 2156 2890 1.34 Comparative Example 3 1987 2643 1.33 Comparative Example 4 1682 2052 1.22 Comparative Example 5 1593 1943 1.22 Comparative Example 6 1876 2458 1.31 Comparative Example 7 1271 1425 1.12 Comparative Example 8 1269 1423 1.12 Comparative Example 9 1267 1420 1.12 Comparative Example 10 1266 1419 1.12 Comparative Example 11 1352 1581 1.17 Comparative Example 12 1327 1546 1.16 Table 2. Results of 24-hour cumulative transdermal absorption rate for each example and comparative sample. Example 1 89.37 Comparative Example 7 72.58 Example 2 86.52 Comparative Example 8 74.16 Example 3 91.25 Comparative Example 9 82.43 Comparative Example 1 26.15 Comparative Example 10 81.79 Comparative Example 2 58.63 Comparative Example 11 76.82 Comparative Example 3 59.74 Comparative Example 12 77.15 Comparative Example 4 71.29 Comparative Example 5 70.85 Comparative Example 6 62.37 Table 3. Free radical scavenging ability test results of each embodiment and comparative sample. Example 1 95.24 96.38 91.75 Example 2 92.16 93.57 88.42 Example 3 97.31 98.02 94.26 Comparative Example 1 62.38 65.74 58.63 Comparative Example 2 78.53 80.26 74.15 Comparative Example 3 79.64 81.35 75.28 Comparative Example 4 83.27 84.69 79.36 Comparative Example 5 82.85 84.12 78.94 Comparative Example 6 75.42 77.15 71.23 Comparative Example 7 88.63 89.74 84.15 Comparative Example 8 87.95 89.03 83.62 Comparative Example 9 90.12 91.26 86.37 Comparative Example 10 89.78 90.85 85.94 Comparative Example 11 84.36 85.71 80.29 Comparative Example 12 84.59 85.93 80.51 Table 4. Results of cellular-level anti-aging efficacy tests for each embodiment and comparative sample. Example 1 138.62 112.47 95.38 Example 2 121.35 98.53 82.64 Example 3 156.79 126.82 108.75 Comparative Example 1 42.37 28.65 31.29 Comparative Example 2 76.52 59.38 52.46 Comparative Example 3 78.64 61.27 54.18 Comparative Example 4 92.15 74.62 68.35 Comparative Example 5 91.78 73.85 67.92 Comparative Example 6 68.39 52.14 46.73 Comparative Example 7 105.26 86.35 78.42 Comparative Example 8 103.78 85.41 77.69 Comparative Example 9 115.43 94.26 84.57 Comparative Example 10 114.69 93.58 83.91 Comparative Example 11 98.52 79.63 72.18 Comparative Example 12 99.17 80.25 72.64 Table 5. Stability test results of each example and comparative sample after 6 months of storage at room temperature. Example 1 Clear and transparent, without layering or sedimentation. No obvious discoloration 2.16 2.38 excellent Example 2 Clear and transparent, without layering or sedimentation. No obvious discoloration 2.34 2.57 excellent Example 3 Clear and transparent, without layering or sedimentation. No obvious discoloration 2.08 2.29 excellent Comparative Example 1 A large amount of sedimentation occurred, resulting in stratification. Severe browning 18.72 42.65 Unqualified Comparative Example 2 Slightly cloudy with a small amount of sediment Obvious browning 8.63 15.72 Unqualified Comparative Example 3 Slightly cloudy with a small amount of sediment Obvious browning 8.47 14.93 Unqualified Comparative Example 4 Clear and transparent, without layering or sedimentation. Slight discoloration 4.28 6.35 qualified Comparative Example 5 Clear and transparent, without layering or sedimentation. Slight discoloration 4.35 6.42 qualified Comparative Example 6 Slightly turbid, with trace amounts of sediment Noticeable color change 7.16 12.84 Unqualified Comparative Example 7 Clear and transparent, without layering or sedimentation. No obvious discoloration 2.21 2.43 excellent Comparative Example 8 Clear and transparent, without layering or sedimentation. No obvious discoloration 2.25 2.46 excellent Comparative Example 9 Clear and transparent, without layering or sedimentation. No obvious discoloration 2.19 2.41 excellent Comparative Example 10 Clear and transparent, without layering or sedimentation. No obvious discoloration 2.20 2.40 excellent Comparative Example 11 Clear and transparent, without layering or sedimentation. Slight discoloration 3.57 4.62 excellent Comparative Example 12 Clear and transparent, without layering or sedimentation. Slight discoloration 3.52 4.58 excellent Table 6. Results of light stability and thermal cycling stability tests for each example and comparative sample. Example 1 3.27 Clarified and transparent, with no abnormalities. 2.84 Example 2 3.46 Clarified and transparent, with no abnormalities. 2.92 Example 3 3.19 Clarified and transparent, with no abnormalities. 2.76 Comparative Example 1 58.39 Severe stratification, large amount of sediment 22.47 Comparative Example 2 21.64 Turbid, with a small amount of sediment 10.35 Comparative Example 3 20.87 Turbid, with a small amount of sediment 10.12 Comparative Example 4 8.53 Clarified and transparent, with no abnormalities. 5.16 Comparative Example 5 8.61 Clarified and transparent, with no abnormalities. 5.23 Comparative Example 6 18.25 Slightly turbid, with trace amounts of sediment 8.74 Comparative Example 7 3.32 Clarified and transparent, with no abnormalities. 2.89 Comparative Example 8 3.35 Clarified and transparent, with no abnormalities. 2.91 Comparative Example 9 3.29 Clarified and transparent, with no abnormalities. 2.86 Comparative Example 10 3.30 Clarified and transparent, with no abnormalities. 2.85 Comparative Example 11 5.72 Clarified and transparent, with no abnormalities. 4.18 Comparative Example 12 5.68 Clarified and transparent, with no abnormalities. 4.15 The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A composition having an anti-aging efficacy, characterized in that, It includes active ingredients from traditional Chinese medicine, moisturizing and penetration-enhancing ingredients, and repairing and anti-aging auxiliary ingredients; The active components of the traditional Chinese medicine are composed of enzymatic hydrolysates of three kinds of traditional Chinese medicine raw materials and fermentation extracts of three kinds of traditional Chinese medicine raw materials; The raw materials for the enzymatic hydrolysate are Cordyceps sinensis, ginseng root, and peony root; The raw materials for the fermented extract are Astragalus root, Rehmannia root, and Angelica root; The enzymatic hydrolysate was prepared by enzymatic hydrolysis using a complex enzyme consisting of cellulase, pectinase, and β-glucanase. The fermentation extract was prepared by anaerobic fermentation using a complex strain of Lactobacillus plantarum, Bifidobacterium, and Lactobacillus acidophilus. The moisturizing and penetration-enhancing component consists of butylene glycol and panthenol; the repairing and anti-aging auxiliary component consists of Centella asiatica root extract and ectoine. By weight, it includes 10-50 parts of active Chinese medicine components, 5-20 parts of moisturizing and penetration-promoting components, 0.5-10 parts of repairing and anti-aging auxiliary components, and the remainder is purified water, with a total weight of 100 parts. By weight, the moisturizing and penetration-enhancing components include 3-15 parts of butylene glycol and 2-5 parts of panthenol; By weight, the repair and anti-aging auxiliary components include 0.3-6 parts of Centella asiatica root extract and 0.2-4 parts of ectoine.

2. The composition having anti-aging efficacy according to claim 1, characterized by, The dosage form of the composition is any one of serum, lotion, cream, toner, and facial mask.

3. The method of preparing the composition having anti-aging efficacy according to claim 1, characterized in that, Includes the following steps: S1. Preparation of enzymatic hydrolysates of traditional Chinese medicine raw materials; S2. Preparation of fermentation extracts from traditional Chinese medicine raw materials; S3. Mix the enzymatic hydrolysate, fermentation extract, moisturizing and penetration-enhancing components, repairing and anti-aging auxiliary components, and purified water in a uniform ratio to obtain the composition.

4. The use of the anti-aging composition according to claim 1 in the preparation of anti-aging and repair cosmetics.