Brucella rapid separation and culture integrated device and method

Abstract

The invention discloses an integrated device and method for rapid separation and culture of brucellosis, and relates to the technical field of germ separation and culture. The Brucella rapid separation and culture integrated device comprises a bottom seat, the gas input pipeline is arranged in the bottom seat and extends to the outer side; the culture base is mounted at the top of the bottom seat; the germ culture medium is mounted at the top of the culture base; the sealing cover body is installed at the top of the culture base, a constant-temperature control structure extending into the bottom base is installed in the culture base, and the constant-temperature control structure comprises a tooth telescopic assembly installed in the center of the interior of the bottom base. According to the Brucella culture device disclosed by the invention, by arranging the recyclable gas capable of respectively carrying out temperature control treatment on the outer side and the inner side of the Brucella, the contact area of the Brucella and the temperature control gas is increased, and the culture effect of the Brucella is ensured. Meanwhile, the problem of accumulation of temperature control gas in a transmission state is avoided, and the brucellosis can be continuously cultured for multiple days.

Description

Technical Field

[0001] This invention relates to the field of pathogen isolation and culture technology, specifically to an integrated device and method for rapid isolation and culture of Brucella bacteria. Background Technology

[0002] Brucella is a Gram-negative bacillus that can infect livestock such as cattle, sheep, and pigs. Humans become infected through contact with infected animals or by consuming contaminated dairy or meat products. The main purpose of Brucella culture is to provide a diagnostic basis for brucellosis by directly isolating and identifying the pathogen, and to support clinical treatment decisions. Isolation of Brucella can only be achieved after culture.

[0003] Existing pathogen isolation and culture devices require special attention to the growth environment for culturing Brucella (within 5%–10% CO2 and a temperature range of 35–37°C). Traditional methods typically employ a flowing gas environment to meet the temperature requirements of Brucella, promoting heat exchange and reducing bacterial accumulation in warmer areas, thus improving cultivation efficiency. However, in practice, this method results in a small contact area between the flowing gas and the Brucella within the culture medium. Brucella bacteria located in the middle section, far from the gas inlet, struggle to come into contact with the suitable temperature airflow, negatively impacting the cultivation outcome. Summary of the Invention

[0004] The purpose of this invention is to provide an integrated device and method for rapid isolation and culture of Brucella bacteria, so as to solve the problems mentioned in the background art.

[0005] To achieve the above-mentioned objectives, the present invention adopts the following technical solution: This invention provides an integrated device for rapid isolation and culture of Brucella bacteria, comprising: a base; a gas input pipe disposed inside the base and extending to the outside; a culture medium holder installed on top of the base; a bacterial culture medium installed on top of the culture medium holder; and a sealing cover installed on top of the culture medium holder. A temperature control structure extending into the base is installed inside the culture medium holder. The constant temperature control structure includes: a toothed telescopic assembly installed at the center of the bottom seat; an intermediate temperature control assembly installed inside the culture medium seat and located on top of the toothed telescopic assembly; and a controllable exhaust assembly installed on top of the toothed telescopic assembly and extending into the intermediate temperature control assembly, wherein the controllable exhaust assembly is rotatably connected to the inside of the sealing cover.

[0006] As a preferred embodiment of the present invention, one end of the gas input pipeline is connected to a telescopic threaded hose extending into the interior of the bottom seat, a compensation pipeline is installed on the outside of the telescopic threaded hose, and a toothed telescopic assembly is installed on the outside of the compensation pipeline.

[0007] As a preferred embodiment of the present invention, the inner center of the culture medium base protrudes upward to form an upper convex ring, and the inner side of the upper convex ring is provided with a pathogen culture medium. The inner eccentric part of the culture medium base is provided with a plurality of through holes, and the through holes are located on the outer side of the upper convex ring.

[0008] As a preferred embodiment of the present invention, the center of the bacterial culture medium protrudes upwards to form a central bulge, and a central temperature control component is provided inside the central bulge. Multiple glass covers are provided at the eccentric part of the interior of the sealing cover, and the glass covers are positioned above the bacterial culture medium and on the side of the central bulge.

[0009] As a preferred embodiment of the present invention, the toothed telescopic assembly includes: a linear drive source installed at the center of the bottom of the base; a rotating rod connected to the output end of the linear drive source and rotatably connected to the center of the interior of the base; a rotating gear installed on the outside of the rotating rod and rotatably connected to the center of the interior of the base; a toothed rod meshing with the front and rear sides of the rotating gear and slidably connected to the interior of the base; and a fixing ring installed and fixed at the end of the toothed rod. The inner side of the fixing ring is fitted with a compensation pipeline.

[0010] As a preferred embodiment of the present invention, a controllable exhaust component extending into the interior of the intermediate temperature control component is installed at the top of the rotating rod, and the intermediate temperature control component is disposed above the rotating rod.

[0011] As a preferred embodiment of the present invention, the intermediate temperature control assembly includes: an intermediate exhaust pipe installed at the center of the culture medium holder and extending into the interior of the intermediate protrusion; a circulation port formed on the side wall of the intermediate exhaust pipe and located at the top of the culture medium holder; and an inlet connecting the left and right sides of the intermediate exhaust pipe and disposed inside the culture medium holder. The air inlet of the input nozzle is located at the bottom of the culture medium base, the internal part of the intermediate exhaust pipe is equipped with a controllable exhaust component, the input nozzle is located at the bottom of the circulation port, and there are two sets of circulation ports, with multiple circulation ports in each set.

[0012] As a preferred embodiment of the present invention, the controllable exhaust assembly includes: a telescopic electric cylinder installed at the bottom of the intermediate exhaust pipe and at the top of the rotating rod; a positioning protrusion connected to the output end of the telescopic electric cylinder; a sealing piston that matches the positioning protrusion and is rotatably connected to the top of the intermediate exhaust pipe; an exhaust port opened inside the sealing piston and matching the circulation port; and a positioning rotating rod connected to the center of the top of the sealing piston and rotatably connected to the center of the interior of the sealing cover. The sealing piston is disposed inside a set of circulation ports at the top.

[0013] This invention also provides a method for rapid isolation and culture of Brucella bacteria, comprising the following steps: S1. Preparation of bacterial culture medium: The extracted Brucella bacteria are injected into the bacterial culture medium through a syringe, ensuring that the pH value is maintained at 6.6–6.8, and aseptic operation is used. Samples with high bacterial count and timing are preferred. S2. Culture of Brucella: Brucella was cultured in a 5%–10% CO2 environment, and the culture temperature was set between 35–37℃ using a constant temperature control structure. During the culture period, the bacteria were observed daily, paying attention to changes in the colony morphology. S3. Temperature control of Brucella: The structure of the intermediate temperature control component and the culture medium seat allows the temperature gas to fill the outside and inside of the culture medium, ensuring that the temperature of Brucella bacteria in all locations inside the culture medium is consistent.

[0014] Compared with existing technologies, one or more of the above technical solutions have the following beneficial effects: 1. In the integrated device and method for rapid isolation and culture of Brucella, when culturing Brucella, temperature-controlled gas is continuously introduced into the outer and inner sides of the culture medium containing Brucella. This ensures sufficient contact between the temperature-controlled gas and the Brucella within the culture medium, increasing the contact area between the Brucella and the temperature-controlled gas and guaranteeing effective culturing. Simultaneously, the flowing gas maintains the culturing temperature and disperses the Brucella, reducing the probability of accumulation in warm areas. Furthermore, the temperature-controlled gas located at the center automatically transfers it to the top of the culture medium when accumulation occurs, preventing gas buildup during transfer and ensuring continuous culturing of Brucella for multiple days. 2. In the integrated device and method for rapid isolation and culture of Brucella, when transferring temperature-controlled gas for Brucella, the position of the exhaust port of the temperature-controlled gas can be adjusted by activating a gear structure. This allows for easy adjustment of the position of the exhaust gas, allowing it to enter either the inside or outside of the bacterial culture medium. Simultaneously, the gear structure, which adjusts the position of the exhaust gas, can also rotate the telescopic electric cylinder and the sealing piston, changing the position and angle of the exhaust port inside the sealing piston. This allows the exhaust port and circulation port to connect or close as needed, achieving automatic venting and circulation of excess temperature-controlled gas and preventing it from clogging the inside of the bacterial culture medium. 3. In the integrated device and method for rapid isolation and culture of Brucella, when Brucella is cultured inside the culture medium, a sealed culture environment is set up, and CO2 is added in a timely manner during the temperature-controlled gas transmission process, so that Brucella in the sealed culture environment is always kept within the range of 5%–10%, which meets the optimal culture state of Brucella. Attached Figure Description

[0015] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an improper limitation of the invention.

[0016] Furthermore, the terms "installation," "setup," "equipped with," "connection," "linking," and "socketing" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral structure; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium, or an internal connection between two devices, components, or parts. Those skilled in the art can understand the specific meaning of these terms in this application based on the specific circumstances.

[0017] Figure 1 This is a schematic cross-sectional view of the entire structure of the present invention; Figure 2 This is a schematic diagram of the structure of the present invention after the overall sealing cover is removed; Figure 3 This is a schematic diagram of the overall main view of the present invention; Figure 4 This is a schematic diagram of the overall frontal cross-section of the present invention; Figure 5 This is an exploded view of the connection between the bottom seat and the bacterial culture medium of the present invention. Figure 6 This is a cross-sectional structural diagram showing the connection between the bottom base and the constant temperature control structure of the present invention; Figure 7 This is the present invention. Figure 6Enlarged structural diagram of region A in the middle; Figure 8 This is a cross-sectional structural diagram showing the connection between the intermediate temperature control component and the controllable exhaust component of the present invention; In the picture: 10. Base seat; 20. Gas inlet pipeline; 201. Telescopic threaded hose; 202. Compensation pipeline; 30. Culture medium stand; 301. Upper convex ring; 302. Through hole; 40. Bacterial culture medium; 401. Central bulge; 50. Sealed cap; 501. Glass cap; 60. Constant temperature control structure; 601. Tooth telescopic assembly; 602. Intermediate temperature control assembly; 603. Controllable exhaust assembly; 6011 Linear drive source; 6012 Rotating rod; 6013 Rotating gear; 6014 Toothed rod; 6015 Fixed ring; 6021, Intermediate exhaust pipe; 6022, Circulation port; 6023, Inlet nozzle; 6031, Telescopic electric cylinder; 6032, Positioning protrusion; 6033, Sealing piston; 6034, Exhaust port; 6035, Positioning rotating rod. Detailed Implementation

[0018] To enable those skilled in the art to better understand the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present application, and not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative effort should fall within the scope of protection of the present application.

[0019] Example 1

[0020] Please see Figures 1-8 The integrated rapid isolation and culture device for Brucella includes a base 10; a gas input pipe 20 disposed inside the base 10 and extending to the outside; a culture medium seat 30 installed on top of the base 10; a bacterial culture medium 40 installed on top of the culture medium seat 30; and a sealing cover 50 installed on top of the culture medium seat 30. A constant temperature control structure 60 extending into the base 10 is installed inside the culture medium seat 30. The constant temperature control structure 60 includes: a toothed telescopic assembly 601 installed at the center inside the base 10; an intermediate temperature control assembly 602 installed inside the culture medium seat 30 and located on top of the toothed telescopic assembly 601; and a controllable exhaust assembly 603 installed on top of the toothed telescopic assembly 601 and extending into the intermediate temperature control assembly 602. The controllable exhaust assembly 603 is rotatably connected to the inside of the sealing cover 50.

[0021] It should be noted that one end of the gas input pipe 20 is connected to a telescopic threaded hose 201 extending into the bottom seat 10. A compensation pipe 202 is installed on the outside of the telescopic threaded hose 201, and a toothed telescopic assembly 601 is installed on the outside of the compensation pipe 202.

[0022] The working principle described above is as follows: During the cultivation and subsequent isolation of Brucella, the Brucella inoculum is first injected into the bacterial culture medium 40 and placed on top of the culture medium base 30. Then, temperature-controlled gas is injected into the culture medium base 30 through the gas input pipe 20. The position of the gas input is adjusted by activating the toothed telescopic component 601, ensuring that the gas can enter the outer or inner side of the bacterial culture medium 40 as needed, fully contacting the Brucella inoculum inside the culture medium 40. This increases the contact area between the Brucella inoculum and the gas, ensuring effective cultivation of the Brucella inoculum. Simultaneously, the controllable exhaust component 603 can be activated to transfer the gas located in the middle section to the top of the bacterial culture medium 40, effectively preventing the temperature-controlled gas from accumulating inside the bacterial culture medium 40.

[0023] For details, please refer to the following: Figure 5 The culture medium base 30 has a protrusion at the center of its interior, forming an upper convex ring 301. The inner side of the upper convex ring 301 is provided with a pathogen culture medium 40. Multiple through holes 302 are opened at the eccentric part of the interior of the culture medium base 30, and the through holes 302 are located on the outer side of the upper convex ring 301.

[0024] In this design, the center of the bacterial culture medium 40 protrudes upwards to form a central protrusion 401. A central temperature control component 602 is installed inside the central protrusion 401. Multiple glass covers 501 are provided at the eccentric part of the interior of the sealing cover 50. The glass covers 501 are positioned above the bacterial culture medium 40 and on the side of the central protrusion 401.

[0025] In the integrated rapid isolation and culture device for Brucella of the present invention, the design of the through-hole 302 allows temperature-controlled gas to be transmitted to the outside of the bacterial culture medium 40. The design of the glass cover 501 facilitates observation of the cultured Brucella.

[0026] For details, please refer to the following: Figure 4 , Figure 6 and Figure 7The toothed telescopic assembly 601 includes: a linear drive source 6011 installed at the center of the bottom of the base 10; a rotating rod 6012 connected to the output end of the linear drive source 6011 and rotatably connected to the center of the interior of the base 10; a rotating gear 6013 installed on the outside of the rotating rod 6012 and rotatably connected to the center of the interior of the base 10; a toothed rod 6014 meshing with the front and rear sides of the rotating gear 6013 and slidably connected inside the base 10; and a fixing ring 6015 installed and fixed at the port of the toothed rod 6014, wherein a compensation pipe 202 is installed and fixed on the inner side of the fixing ring 6015.

[0027] In this design, a controllable exhaust assembly 603 extending into the interior of the intermediate temperature control assembly 602 is installed on the top of the rotating rod 6012, and the intermediate temperature control assembly 602 is located above the rotating rod 6012.

[0028] In the integrated rapid isolation and culture device for Brucella of the present invention, when the angle of the temperature-controlled gas transmission is adjusted, the linear drive source 6011 is activated, driving the rotating rod 6012 connected to the output end of the linear drive source 6011 to rotate, and driving the rotating gear 6013 mounted on the outside of the rotating rod 6012 to rotate. When the rotating gear 6013 rotates, the toothed rods 6014 connected to its front and rear sides move towards each other, causing the fixed ring 6015 connected to the toothed rod 6014 to move, stretching the compensation pipe 202 connected to the fixed ring 6015, and changing the position of the outlet of the compensation pipe 202.

[0029] When the compensation pipe 202 is located at the bottom of the intermediate temperature control component 602, the gas will drift downwards and enter the interior of the intermediate temperature control component 602; when the compensation pipe 202 is not located at the bottom of the intermediate temperature control component 602, the gas will enter the outside of the pathogen culture medium 40 through the through hole 302.

[0030] For details, please refer to the following: Figure 6 , Figure 7 and Figure 8 The intermediate temperature control component 602 includes: an intermediate exhaust pipe 6021 installed at the center of the culture medium holder 30 and extending into the interior of the intermediate protrusion 401; a circulation port 6022 located on the side wall of the intermediate exhaust pipe 6021 and at the top of the culture medium holder 30; and an inlet 6023 connected to the left and right sides of the intermediate exhaust pipe 6021 and located inside the culture medium holder 30. The air inlet of the inlet 6023 is located at the bottom of the culture medium holder 30. A controllable exhaust component 603 is installed inside the intermediate exhaust pipe 6021. The inlet 6023 is located at the bottom of the circulation port 6022. There are two sets of circulation ports 6022, and each set of circulation ports 6022 has multiple ports.

[0031] In the integrated rapid isolation and culture device for Brucella of the present invention, when gas enters the inlet 6023, the inlet 6023 will drive the gas transported inside into the intermediate exhaust pipe 6021, and then transport it to the center of the bacterial culture medium 40 through the circulation port 6022 opened inside the intermediate exhaust pipe 6021. When there is a large amount of gas in the center of the bacterial culture medium 40, the gas can enter the intermediate exhaust pipe 6021 through the circulation port 6022 opened at the top, which facilitates the venting and circulation of the gas through the intermediate exhaust pipe 6021.

[0032] For details, please refer to the following: Figure 6 , Figure 7 and Figure 8 The controllable exhaust assembly 603 includes: a telescopic electric cylinder 6031 installed at the bottom of the intermediate exhaust pipe 6021 and at the top of the rotating rod 6012; a positioning protrusion 6032 connected to the output end of the telescopic electric cylinder 6031; a sealing piston 6033 matched with the positioning protrusion 6032 and rotatably connected to the top of the intermediate exhaust pipe 6021; an exhaust port 6034 opened inside the sealing piston 6033 and matched with the circulation port 6022; and a positioning rotating rod 6035 connected to the top center of the sealing piston 6033 and rotatably connected to the inside center of the sealing cover 50, wherein the sealing piston 6033 is disposed inside a set of circulation ports 6022 at the top.

[0033] In the integrated rapid isolation and culture device for Brucella of the present invention, when it is necessary to vent and circulate the gas transferred to the center of the bacterial culture medium 40, the telescopic electric cylinder 6031 is first activated, driving the positioning protrusion 6032 connected to the output end of the telescopic electric cylinder 6031 to move up and down, and connecting the positioning protrusion 6032 to the sealing piston 6033. At this time, the rotation of the rotating rod 6012 will drive the telescopic electric cylinder 6031 and the positioning protrusion 6032 to rotate, and drive the sealing piston 6033 to rotate, so that the exhaust port 6034 and the circulation port 6022 inside the sealing piston 6033 are connected. The gas transferred to the circulation port 6022 can be transferred to the outside of the intermediate exhaust pipe 6021 through the exhaust port 6034, realizing the venting and circulation operation of the temperature-controlled gas.

[0034] Example 2

[0035] Please see Figures 1-8 A rapid isolation and culture method for Brucella bacteria includes the following steps: S1. Preparation of bacterial culture medium 40: The extracted Brucella bacteria are injected into the bacterial culture medium 40 through a syringe, ensuring that the pH value is maintained at 6.6–6.8, and aseptic operation is used, giving priority to specimens with high bacterial count and timing; S2. Culture of Brucella: Brucella was cultured in a 5%–10% CO2 environment, and the culture temperature was set between 35–37℃ using a constant temperature control structure 60. Observation was performed daily during the culture period, paying attention to changes in the colony morphology of Brucella. S3. Temperature control of Brucella bacteria: The structure of the intermediate temperature control component 602 and the culture medium seat 30 allows the temperature gas to fill the outside and inside of the bacterial culture medium 40, ensuring that the temperature of Brucella bacteria in all positions inside the bacterial culture medium 40 is consistent.

[0036] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely preferred examples and are not intended to limit the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

[0037] The terms “center,” “longitudinal,” “lateral,” “front,” “rear,” “left,” “right,” “vertical,” “horizontal,” “top,” “bottom,” “inner,” and “outer,” etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are merely simplified descriptions for the convenience of describing the present invention and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting the scope of protection of the present invention.

[0038] Therefore, any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in this invention, based on the technical solution and inventive concept of this invention, should be covered within the protection scope of this invention.

Claims

1. An integrated device for rapid isolation and culture of Brucella bacteria, characterized in that, include: Bottom seat (10); gas inlet pipe (20) disposed inside the bottom seat (10) and extending to the outside; culture medium seat (30) installed on top of the bottom seat (10); pathogen culture medium (40) installed on top of the culture medium seat (30); sealing cover (50) installed on top of the culture medium seat (30), wherein a constant temperature control structure (60) extending into the bottom seat (10) is installed inside the culture medium seat (30). The constant temperature control structure (60) includes: a toothed telescopic assembly (601) installed at the center inside the bottom seat (10); an intermediate temperature control assembly (602) installed inside the culture medium seat (30) and located on top of the toothed telescopic assembly (601); and a controllable exhaust assembly (603) installed on top of the toothed telescopic assembly (601) and extending into the intermediate temperature control assembly (602), wherein the controllable exhaust assembly (603) is rotatably connected to the inside of the sealing cover (50).

2. The integrated device for rapid isolation and culture of Brucella bacteria according to claim 1, characterized in that: One end of the gas input pipe (20) is connected to a telescopic threaded hose (201) extending into the bottom seat (10). A compensation pipe (202) is installed on the outside of the telescopic threaded hose (201), and a toothed telescopic assembly (601) is installed on the outside of the compensation pipe (202).

3. The integrated device for rapid isolation and culture of Brucella bacteria according to claim 1, characterized in that: The culture medium base (30) protrudes upward from the center of the interior and forms an upper convex ring (301). The inner side of the upper convex ring (301) is provided with a pathogen culture medium (40). Multiple through holes (302) are opened at the eccentric part of the interior of the culture medium base (30). The through holes (302) are located on the outer side of the upper convex ring (301).

4. The integrated device for rapid isolation and culture of Brucella bacteria according to claim 2, characterized in that: The bacterial culture medium (40) has a central protrusion that extends upwards and forms a central protrusion (401). A central temperature control component (602) is provided inside the central protrusion (401). Multiple glass covers (501) are provided at the eccentric part of the interior of the sealing cover (50). The glass covers (501) are positioned above the bacterial culture medium (40) and on the side of the central protrusion (401).

5. The integrated device for rapid isolation and culture of Brucella bacteria according to claim 2, characterized in that: The toothed telescopic assembly (601) includes: a linear drive source (6011) installed at the center of the bottom of the base (10); a rotating rod (6012) connected to the output end of the linear drive source (6011) and rotatably connected to the center of the interior of the base (10); a rotating gear (6013) installed on the outside of the rotating rod (6012) and rotatably connected to the center of the interior of the base (10); a toothed rod (6014) meshing with the front and rear sides of the rotating gear (6013) and slidably connected inside the base (10); and a fixing ring (6015) installed and fixed at the port of the toothed rod (6014). The inner side of the fixing ring (6015) is fitted with a compensation pipe (202).

6. The integrated device for rapid isolation and culture of Brucella bacteria according to claim 5, characterized in that: The top of the rotating rod (6012) is equipped with a controllable exhaust assembly (603) that extends into the interior of the intermediate temperature control assembly (602), and the intermediate temperature control assembly (602) is disposed above the rotating rod (6012).

7. The integrated device for rapid isolation and culture of Brucella bacteria according to claim 6, characterized in that: The intermediate temperature control assembly (602) includes: an intermediate exhaust pipe (6021) installed at the center inside the culture medium holder (30) and extending into the interior of the intermediate protrusion (401); a circulation port (6022) opened on the side wall of the intermediate exhaust pipe (6021) and located at the top of the culture medium holder (30); and an inlet (6023) connecting the left and right sides of the intermediate exhaust pipe (6021) and disposed inside the culture medium holder (30). The air inlet of the input nozzle (6023) is located at the bottom of the culture medium base (30), and a controllable exhaust assembly (603) is installed inside the intermediate exhaust pipe (6021). The input nozzle (6023) is located at the bottom of the circulation port (6022), and there are two sets of circulation ports (6022), with multiple circulation ports (6022) in each set.

8. The integrated device for rapid isolation and culture of Brucella bacteria according to claim 7, characterized in that: The controllable exhaust assembly (603) includes: a telescopic electric cylinder (6031) installed at the bottom inside the intermediate exhaust pipe (6021) and at the top inside the rotating rod (6012); a positioning protrusion (6032) connected to the output end of the telescopic electric cylinder (6031); a sealing piston (6033) matching the positioning protrusion (6032) and rotatably connected to the top inside the intermediate exhaust pipe (6021); an exhaust port (6034) opened inside the sealing piston (6033) and matching the circulation port (6022); and a positioning rotating rod (6035) connected at the top center of the sealing piston (6033) and rotatably connected at the inside center of the sealing cover (50). The sealing piston (6033) is disposed inside a set of circulation ports (6022) at the top.

9. A method for rapid isolation and culture of Brucella, specifically the integrated device for rapid isolation and culture of Brucella as described in any one of claims 1-8, characterized in that, Includes the following steps: S1. Preparation of bacterial culture medium (40): The extracted Brucella bacteria are injected into the bacterial culture medium (40) through a syringe, ensuring that the pH value is maintained at 6.6–6.8, and aseptic operation is used, giving priority to specimens with high bacterial count and timing; S2. Culture of Brucella: Brucella was cultured in a 5%–10% CO2 environment, and the culture temperature was set between 35–37℃ using a constant temperature control structure (60). During the culture period, the bacteria were observed daily, and attention was paid to the changes in the colony morphology of Brucella. S3. Temperature control of Brucella: The temperature gas is filled to the outside and inside of the bacterial culture medium (40) through the structure of the intermediate temperature control component (602) and the culture medium seat (30), so as to ensure that the temperature of Brucella in all positions inside the bacterial culture medium (40) is consistent.

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