Haematococcus culture solution heavy metal passivation device based on chelating agent

Abstract

The invention relates to the technical field of haematococcus culture, in particular to a chelating agent-based haematococcus culture solution heavy metal passivation device which comprises a buffer tank for collecting a culture solution, a power box arranged below the bottom of the buffer tank and a stirring barrel arranged in the buffer tank, a plurality of feeding barrels are arranged at the top of the buffer tank and are configured into an acid and alkali liquor feeding barrel, a chelating agent feeding barrel and a flocculating agent feeding barrel; the device further comprises a central control unit, and the central control unit controls the amount of the chelating agent released by the chelating agent feeding barrel according to detection information of the heavy metal concentration sensor. By arranging the integrated buffer tank and the step-by-step adding system, pretreatment and precise reaction of the culture solution are completed, and the effect of preventing heavy metal poison from the source is achieved; by arranging a linkage mechanism of the detection tank and the return pipeline, the technical effect of terminal verification and guarantee of the effluent quality is achieved.

Description

Technical Field

[0001] This invention relates to the field of Haematococcus pluvialis cultivation technology, specifically to a heavy metal passivation device for Haematococcus pluvialis culture medium based on a chelating agent. Background Technology

[0002] As the optimal source of natural astaxanthin, the purity and safety of large-scale cultivation technology for Haematococcus pluvialis are of paramount importance. During cultivation, the introduction of heavy metal ions such as Pb and Cd is an unavoidable risk. These heavy metals not only inhibit algal cell growth and astaxanthin accumulation but also ultimately accumulate in the algal powder, affecting product quality and safety.

[0003] The invention patent application with application number CN201210053019.3 discloses a method for controlling heavy metals in the cultivation of Haematococcus pluvialis, including the following steps: 1) mixing Haematococcus pluvialis mud and cleaning agent at a mass-volume ratio of 1:1 to 1:8; 2) placing the mixture obtained in step 1) on a shaker and washing for 1-15 hours; 3) centrifuging the mixture after washing in step 2), removing the supernatant, and collecting the algal mud; 4) washing the algal mud collected in step 3) with water 2-3 times; 5) centrifuging the algal mud after washing in step 4) and collecting the algal mud; 6) drying and pulverizing the algal mud collected in step 5) and detecting its Pb and Cd contents; the method of the present invention is applied to the production of Haematococcus pluvialis powder, which can control the Pb content of Haematococcus pluvialis powder to below 2.0 mg / Kg and the Cd content to below 0.1 mg / Kg; the astaxanthin content was not significantly affected by spectrophotometry, and the cis- and trans-astaxanthin structures were not significantly changed by HPLC.

[0004] Existing technologies offer a post-harvest treatment approach. This method involves repeatedly mixing, washing, and centrifuging the algal sludge with a cleaning agent after harvesting to remove heavy metals adsorbed on the algal surface and accumulated internally. However, this method cannot prevent the continued toxic effects of heavy metals on algal cell growth already occurring during cultivation, potentially leading to prolonged cultivation periods and reduced biomass.

[0005] Therefore, existing technologies lack a dedicated heavy metal passivation device that can be integrated into the front end of the Haematococcus pluvialis culture process to achieve automated, precise, and efficient processing while ensuring the initial purity of the culture medium. Summary of the Invention

[0006] In order to overcome the defects in the prior art, the purpose of this invention is to provide a heavy metal passivation device for Haematococcus pluvialis culture medium based on chelating agents. The core of this invention is to move the heavy metal removal process forward, purifying and passivating the culture medium before it is put into use, so as to solve the problems mentioned in the background art.

[0007] To achieve the above objectives, the present invention provides a heavy metal passivation device for Haematococcus pluvialis culture medium based on a chelating agent, comprising a buffer tank for collecting the culture medium, a power box disposed below the bottom of the buffer tank, and a stirring drum disposed inside the buffer tank; the power box is equipped with a motor and the motor is coaxially connected to the stirring drum; the stirring drum divides the inner cavity of the buffer tank into inner and outer chambers, wherein a spiral infusion plate is coaxially disposed inside the stirring drum to convect and stir the culture medium inside the buffer tank;

[0008] The bottom of the buffer tank is provided with a truncated cone-shaped slag collection platform. The cone surface of the slag collection platform has several slag discharge ports that are evenly spaced in a ring. The bottom surface of the slag collection platform is fitted with several movable caps in a ring shape for sealing and opening the slag discharge ports.

[0009] The top of the buffer tank is provided with several feeding buckets, which are configured as acid and alkali feeding buckets, chelating agent feeding buckets and flocculant feeding buckets.

[0010] The device also includes a central control unit, which is connected to a heavy metal concentration sensor and a flow sensor located at the inlet of the buffer tank. The central control unit controls the amount of chelating agent released from the chelating agent feeding tank based on the detection information from the heavy metal concentration sensor.

[0011] The above-described approach differs fundamentally from existing technologies, seeking a preventative solution. Its core lies in pre-emptively removing heavy metals, purifying and passivating the culture medium before its use. While chelating agents are widely used in industrial wastewater treatment to remove heavy metals, their equipment and processes are not designed for biological culture systems with extremely high purity requirements. They suffer from low processing precision, insufficient automation, and a tendency to cause secondary pollution, making them unsuitable for direct application in Haematococcus pluvialis cultivation. Unlike existing technologies that passively clean the algae after harvest, this invention purifies the culture medium from the outset, actively eliminating the inhibitory and toxic effects of heavy metals on Haematococcus pluvialis throughout its growth cycle. This provides an optimal growth environment for algal cells, fundamentally ensuring biomass and astaxanthin yield. This invention abandons the traditional experience-based manual dosing method, achieving on-demand, precise, and automated dosing of chelating agents and acids / bases. This not only ensures complete passivation of heavy metals but also eliminates potential secondary damage to algal cells due to excessive dosage, while simultaneously reducing operating costs.

[0012] As a further improvement to this technical solution, the central control unit is also connected to a pH sensor installed on the inner wall of the buffer tank, and controls the amount of acid and alkali added to the acid and alkali feeding tank according to a preset pH range.

[0013] As a further improvement to this technical solution, the size of the movable cover is larger than the size of the slag discharge port and is equal to the size of the conical surface between two adjacent slag discharge ports. A linkage frame is slidably engaged between several movable covers. The linkage frame is sleeved and movable with the motor output shaft. A pair of electric push rods are installed inside the top of the power box to drive the linkage frame to rise and fall and switch between engaging and disengaging with several movable covers.

[0014] As a further improvement to this technical solution, a scraper is vertically engaged between the slag accumulation platform and the side wall of the buffer tank, thereby dividing the bottom cavity of the buffer tank into several sedimentation cavities; the scraper is fixedly connected to the top surface of one side of the movable cover by a connecting rod, and the scraper has a structure that is wider at the bottom and narrower at the top.

[0015] As a further improvement to this technical solution, a locking block is provided in the middle of the concave arc surface of the movable cover. The locking block is engaged with the outer end of the linkage frame. The center of the linkage frame is provided with a ring and the inner wall of the ring is provided with several vertical slots. The outer wall of the output shaft of the motor is provided with several vertical ribs, which are correspondingly engaged with several slots.

[0016] As a further improvement to this technical solution, a rotating ring is fixedly provided on the bottom surface of the circular ring at the center of the linkage frame. An annular groove is opened on the bottom surface of the rotating ring, and the longitudinal section of the annular groove is a cavity that is wider at the top and narrower at the bottom. A top rod is fixedly connected to the top of the electric push rod, and the top of the top rod is adapted to slide and engage with the annular groove.

[0017] The above setup, through the unique design of a frustum-shaped slag accumulation platform, a movable cover, and a scraper, allows heavy metal precipitates to slide down naturally and concentrate at the slag discharge port. During slag discharge, the cover is automatically opened and the residue is scraped off through mechanical linkage, achieving easy and thorough discharge of precipitates. This overcomes the drawbacks of traditional tanks that require manual cleaning or the use of additional pumps, greatly improving the efficiency and convenience of continuous operation of the equipment.

[0018] As a further improvement to this technical solution, the outer wall of the stirring cylinder is provided with a number of stirring rods at equal intervals in a ring shape, and a number of liquid-repellent holes are opened on the side of the stirring rods. A fixing frame is fixedly provided on the top of the stirring cylinder, and the middle part of the fixing frame is fixedly connected to the top of the spiral infusion plate.

[0019] The above setup, through the design of the hydrophobic holes on the stirring rod, makes the mixing process gentler, avoiding damage to potentially beneficial components in the culture medium or subsequent algal cells to be inoculated by severe shear force, ensuring efficient mixing while taking into account the special requirements of biocompatibility.

[0020] As a further improvement to this technical solution, a drain nozzle is installed on the side wall of the buffer tank at the same height as the slag accumulation platform, and a solenoid valve is installed on the drain nozzle; the drain nozzle is connected to a detection tank, and a heavy metal concentration sensor is installed inside the detection tank, which is signal-connected to the central control unit.

[0021] As a further improvement to this technical solution, a reflux pipeline is provided between the detection tank and the buffer tank. One end of this reflux pipeline is connected to the bottom of the detection tank, and the other end is connected to the top side wall of the buffer tank. A reflux pump and a solenoid valve are provided on this reflux pipeline. The solenoid valve is controlled by the central control unit. When the detection value of the heavy metal concentration sensor in the detection tank exceeds the standard, the central control unit controls the opening of this solenoid valve and starts the reflux pump to pump the liquid in the detection tank back to the buffer tank for further processing.

[0022] The above setup, through the linkage mechanism between the testing tank and the return pipeline, completes the final verification and assurance of the effluent water quality. This invention adds a "quality inspection station" before the final effluent; if the test fails, the system can automatically return the liquid for reprocessing. This self-verification and correction mechanism ensures the absolute purity of the produced culture medium, providing the most reliable foundation for stable and high-quality Haematococcus pluvialis cultivation.

[0023] As a further improvement to this technical solution, an electromagnetic valve is installed on the connecting pipe between the feeding tank and the buffer tank, which is controlled to start and stop by the central control unit. The flocculant is added to the feeding tank and forms a precipitate in conjunction with the chelating agent. A liquid level sensor is installed on the inner wall of the buffer tank at a height such as the slag accumulation platform. The central control unit coordinates the start and stop of the electromagnetic valves on several feeding tanks, drain nozzles and return pipelines based on the signal from the liquid level sensor.

[0024] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0025] 1. This heavy metal passivation device for Haematococcus pluvialis culture medium based on chelating agents, through the setting of an integrated buffer tank and a step-by-step dosing system, completes the pretreatment and precise reaction of the culture medium, thereby achieving the technical effect of preventing heavy metal toxicity from the source and improving culture efficiency; through the setting of a detection tank and a return pipeline linkage mechanism, the terminal verification and guarantee of the effluent water quality are completed, thereby achieving the technical effect of preventing unqualified culture medium from flowing into the next stage.

[0026] 2. This heavy metal passivation device for Haematococcus pluvialis culture medium based on chelating agents achieves intelligent closed-loop control of reagent addition by linking heavy metal concentration sensors and pH sensors through a central control unit. This results in precise and efficient treatment while avoiding reagent waste and secondary pollution.

[0027] 3. This heavy metal passivation device for Haematococcus pluvialis culture medium based on chelating agents, through the unique design of a frustum-shaped slag accumulation platform, movable cover and slag scraper, automatically opens the cover and scrapes away the residue through mechanical linkage during slag discharge, realizing easy and thorough discharge of sediment. It overcomes the drawbacks of traditional tanks that require shutdown for manual cleaning or the use of additional pump suction equipment, and greatly improves the efficiency and convenience of continuous operation of the equipment.

[0028] 4. This heavy metal passivation device for Haematococcus pluvialis culture medium based on chelating agent achieves gentle and efficient convection stirring of the culture medium through the unique structural design of the stirring tank and spiral infusion plate, thus achieving the technical effect of uniform mixing of reactants, full reaction and avoiding damage to liquid components. Attached Figure Description

[0029] The accompanying drawings described herein are for illustrative purposes only and are not intended to limit the scope of the invention in any way. Furthermore, the shapes and proportions of the components in the drawings are merely illustrative to aid in understanding the invention and do not specifically limit the shapes and proportions of the components. Those skilled in the art, guided by the teachings of this invention, will select various possible shapes and proportions to implement the invention according to specific circumstances.

[0030] Figure 1 This is a schematic diagram of the overall assembly structure of the present invention;

[0031] Figure 2 This is a schematic diagram of the internal assembly structure of the buffer container of the present invention;

[0032] Figure 3 This is a schematic diagram of the buffer tank structure of the present invention;

[0033] Figure 4 This is a schematic diagram of the overall assembly structure for removing the buffer container according to the present invention;

[0034] Figure 5 This is a schematic diagram of the spiral infusion plate assembly structure of the present invention;

[0035] Figure 6 This is a schematic diagram of the assembly structure of the stirring tank of the present invention;

[0036] Figure 7 This is a split view of several movable caps of the present invention;

[0037] Figure 8 This is a schematic diagram of the linkage frame assembly structure of the present invention;

[0038] Figure 9 This is a schematic diagram of the linkage frame structure of the present invention;

[0039] The meanings of the labels in the diagram are as follows:

[0040] 100. Buffer tank; 110. Slag collection platform; 111. Slag discharge port; 120. Feeding bucket; 130. Liquid discharge nozzle;

[0041] 200. Power box;

[0042] 300. Stirring drum; 310. Spiral infusion plate; 320. Vertical rib; 330. Stirring rod; 331. Drainage hole; 340. Fixing frame;

[0043] 400. Movable cover; 401. Locking block; 402. Connecting rod; 410. Slag scraper; 420. Linkage frame; 421. Locking groove; 422. Rotary ring; 4221. Annular groove; 423. Top rod; 424. Electric push rod; 430. Pressure ring; 440. Support ring. Detailed Implementation

[0044] The details of the present invention can be more clearly understood by referring to the accompanying drawings and the description of specific embodiments. However, the specific embodiments of the present invention described herein are for illustrative purposes only and should not be construed as limiting the invention in any way. Under the teachings of this invention, those skilled in the art will conceive of any possible variations of the invention, all of which should be considered within the scope of the invention. The terms "installation" and "connection" should be interpreted broadly, referring to direct connection as well as indirect connection through an intermediate medium.

[0045] The terms "central axis," "vertical," "horizontal," "front," "rear," "upper," "lower," "left," "right," "top," "bottom," "inner," and "outer" used herein to indicate orientation or positional relationships are based on the orientation or positional relationships shown in the accompanying drawings and are used only for the convenience of describing the invention and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the invention. Furthermore, in the description of the invention, "a number" means two or more, unless otherwise explicitly specified.

[0046] Please see Figures 1-6 As shown, the present invention provides a heavy metal passivation device for Haematococcus pluvialis culture medium based on chelating agents, including a buffer tank 100 for collecting the culture medium, a power box 200 disposed below the bottom of the buffer tank 100, and a stirring drum 300 disposed inside the buffer tank 100; the power box 200 is equipped with a motor and is coaxially connected to the stirring drum 300; the stirring drum 300 divides the inner cavity of the buffer tank 100 into inner and outer chambers, wherein a spiral infusion plate 310 is coaxially disposed inside the stirring drum 300 to convect and stir the culture medium inside the buffer tank 100, so as to achieve thorough stirring and save time;

[0047] The top of the buffer tank 100 is equipped with several feeding tanks 120, which are configured as acid and alkali feeding tanks, chelating agent feeding tanks, and flocculant feeding tanks. The acid and alkali feeding tanks are used to adjust the pH value of the culture medium to a pH range suitable for the optimal function of the chelating agent. The chelating agent reacts strongly with heavy metal ions to form insoluble and stable chelates, which can then be separated from the culture medium. The flocculant is added after the heavy metals have been passivated, causing the fine chelate precipitates in the water to aggregate into larger flocs, which then settle rapidly, achieving efficient solid-liquid separation.

[0048] The device also includes a central control unit, which is connected to a heavy metal concentration sensor and a flow sensor located at the inlet of the buffer tank 100. The central control unit controls the amount of chelating agent released from the chelating agent feeding tank according to the detection information of the heavy metal concentration sensor, so that it completely passivates and precipitates the heavy metals in the culture medium.

[0049] Specifically, the bottom of the buffer tank 100 is provided with a truncated cone-shaped slag accumulation platform 110, which allows the precipitated heavy metals to accumulate in the space between the slag accumulation platform 110 and the bottom of the buffer tank 100. The cone surface of the slag accumulation platform 110 is provided with several slag discharge ports 111 at equal intervals in a ring shape. Since the precipitated heavy metals accumulate on the inclined cone surface of the slag accumulation platform 110, the slag discharge ports 111 can be opened after the supernatant of the buffer tank 100 is released, and the precipitated heavy metals can be discharged by their own weight without the need for suction discharge, which has the effect of reducing costs and increasing efficiency.

[0050] Furthermore, the central control unit is also connected to a pH sensor installed on the inner wall of the buffer tank 100, and controls the amount of acid and alkali added to the acid and alkali feeding tanks according to the preset pH range; the pH value of the culture medium is precisely stabilized within the optimal range for the chelating agent to play its role, usually a weakly alkaline environment, such as 7.5-9.0, thereby ensuring the efficient progress of the chelation reaction.

[0051] Furthermore, a drain nozzle 130 is installed on the side wall of the buffer tank 100 at the same height as the slag accumulation platform 110, and a solenoid valve is installed on the drain nozzle 130; the drain nozzle 130 is connected to a detection tank, and a heavy metal concentration sensor is installed inside the detection tank, which is connected to the central control unit.

[0052] Specifically, to ensure that the purified culture medium discharged from the buffer tank 100 meets the standards, this quality monitoring and assurance system is implemented. A reflux pipeline is installed between the testing tank and the buffer tank 100. One end of this reflux pipeline connects to the bottom of the testing tank, and the other end connects to the top side wall of the buffer tank 100. This reflux pipeline is equipped with a reflux pump and a solenoid valve. This solenoid valve 72 is controlled by the central control unit. When the heavy metal concentration sensor in the testing tank exceeds the standard, the central control unit controls the opening of this solenoid valve and starts the reflux pump to return the liquid in the testing tank to the buffer tank 100 for further processing. After purification in the buffer tank 100, the water quality is tested again. If the detection value exceeds the standard, the central control unit will immediately activate the reflux pipeline to pump the unqualified culture medium back to the buffer tank 100 for secondary treatment, eliminating the risk of unqualified culture medium entering the next production stage.

[0053] Furthermore, a solenoid valve is installed on the connecting pipe between the feeding tank 120 and the buffer tank 100. The valve is controlled to start and stop by the central control unit. The flocculant is added to the feeding tank and forms a precipitate in conjunction with the chelating agent. After the chelation reaction is completed, the flocculant is added to the liquid flow, causing the small chelate precipitate particles in the water to agglomerate into larger flocs, which then settle rapidly on the conical surface of the slag collection platform 110, achieving efficient solid-liquid separation. A liquid level sensor is installed on the inner wall of the buffer tank 100 at the same height as the slag collection platform 110. The central control unit coordinates the start and stop of the solenoid valves on several feeding tanks 120, drain nozzles 130, and return pipelines based on the signal from the liquid level sensor.

[0054] Furthermore, the outer wall of the stirring tank 300 is provided with a number of stirring rods 330 at equal intervals in a ring shape. The sides of the stirring rods 330 are provided with a number of hygroscopic holes 331, so that when the stirring rods 330 stir the culture medium, they will not damage the liquid components by impact, but will flow and mix through the hygroscopic holes 331. The top of the stirring tank 300 is fixedly provided with a fixing frame 340, and the middle part of the fixing frame 340 is fixedly connected to the top of the spiral infusion plate 310, so that the stirring tank 300 and the spiral infusion plate 310 rotate synchronously and are stirred when the culture medium flows up and down, so that the culture medium is fully mixed with the chelating agent, achieving rapid reaction and precipitation of heavy metals.

[0055] like Figures 7-9 As shown, in order to enable automatic control of the closure and opening of the slag discharge port 111, the bottom surface of the slag accumulation platform 110 is ring-shaped with several movable covers 400 for sealing and opening the slag discharge port 111. The size of the movable cover 400 is larger than the size of the slag discharge port 111 and is equal to the size of the conical surface between two adjacent slag discharge ports 111, so that the movable cover 400 completely seals the slag discharge port 111. At the same time, when the movable cover 400 is rotated to overlap with the outside of the conical surface of the slag accumulation platform 110, the slag discharge port 111 is fully opened.

[0056] Furthermore, in order to completely remove the sediment retained on the inner side of the slag accumulation platform 110, a scraper 410 is vertically engaged between the slag accumulation platform 110 and the side wall of the buffer tank 100, dividing the bottom cavity of the buffer tank 100 into several sedimentation cavities so that the culture medium and chelating agent can be fully and evenly mixed, and the heavy metal deposits can be evenly distributed, facilitating subsequent cleaning. The scraper 410 is fixedly connected to the top surface of one side of the movable cover 400 by a connecting rod 402. The scraper 410 has a structure that is wider at the bottom and narrower at the top, so that when the slag discharge port 111 is fully opened, the scraper 410 moves to the side of the slag discharge port 111 along with the movable cover 400, thereby scraping off the heavy metal deposits on the conical surface of the slag accumulation platform 110.

[0057] Specifically, to allow the movable covers 400 to automatically switch between opening and closing, a linkage frame 420 is slidably engaged between several movable covers 400. The linkage frame 420 is sleeved with the motor output shaft. A pair of electric push rods 424 are installed inside the top of the power box 200 to drive the linkage frame 420 to rise and fall, switching between engaging and disengaging with the several movable covers 400. Pressure rings 430 and support rings 440 are fixedly installed on the upper and lower sides of the slag accumulation platform 110, respectively, to support the upper and lower ends of the several movable covers 400 to fit against the outer wall of the slag accumulation platform 110. By embedding sealing strips on the inner side of the movable covers 400, the buffer tank 100 is kept sealed when the movable covers 400 block the slag discharge port 111.

[0058] Furthermore, a locking block 401 is provided in the middle of the concave arc surface of the movable cover 400. The locking block 401 is engaged with the outer end of the linkage frame 420. The center of the linkage frame 420 is provided with a circular ring and the inner wall of the circular ring is provided with several vertically oriented slots 421. The outer wall of the motor output shaft is provided with several vertical ribs 320, which are correspondingly sleeved with several slots 421, so that the linkage frame 420 is driven by the motor and deflects to open the movable cover 400 after being engaged with the movable cover 400.

[0059] It is worth noting that a rotating ring 422 is fixedly provided on the bottom surface of the circular ring at the center of the linkage frame 420. The bottom surface of the rotating ring 422 has an annular groove 4221, and the longitudinal section of the annular groove 4221 is a cavity that is wider at the top and narrower at the bottom, such as a T-shape or an inverted triangle shape. The top of the electric push rod 424 is fixedly connected to a top rod 423. The top of the top rod 423 is adapted to slide and engage with the annular groove 4221, so that the top rod 423 does not rotate with the rotating ring 422, while it can drive the rotating ring 422 and the linkage frame 420 to rise and fall as a whole, so as to facilitate the engagement and disengagement of the movable cover 400, thereby controlling the closing and opening of the movable cover 400 and the slag discharge port 111.

[0060] The method of using the heavy metal passivation device for Haematococcus pluvialis culture medium based on chelating agents of the present invention includes the following steps:

[0061] S1. Pump the culture medium to be processed into the buffer tank 100 through the feeding tank 120;

[0062] S2. The central control unit calculates the theoretical total dosage of the chelating agent based on the reading of the heavy metal concentration sensor at the inlet of the buffer tank 100, and releases it.

[0063] S3. The central control unit controls the feeding tank 120 containing acid and alkali solutions to release the acid and alkali solutions based on the feedback of the pH sensor on the inner wall of the buffer tank 100, thereby adjusting the pH value to a weakly alkaline range of 7.5-9.0, which is suitable for the optimal pH range for the chelating agent to function.

[0064] S4. The central control unit controls the motor to start and drive the stirring drum 300 and the spiral infusion plate 310 to rotate synchronously. Stirring is carried out when the culture medium is in convection, so that the culture medium is fully mixed with the chelating agent, and heavy metals are rapidly reacted and settled.

[0065] S5. After the chelation reaction is completed, the flocculant is added to the liquid through the flocculant feeding tank by the central control unit, so that the small chelate precipitate particles in the water agglomerate into larger flocs, and then settle rapidly on the cone-shaped surface of the slag collection platform 110 to achieve efficient solid-liquid separation.

[0066] S6. Open the drain nozzle 130 to drain the supernatant and transfer it to the purified storage tank for later use.

[0067] It should be noted that the fixed connections and fixing methods of the present invention are achieved using conventional fixing means such as bolt connections or welding. The above embodiments are only for illustrating the technical concept and features of the present invention, and are intended to enable those skilled in the art to understand the content of the present invention and implement it accordingly, and should not be used to limit the scope of protection of the present invention. All equivalent changes or modifications made according to the spirit and essence of the present invention should be covered within the scope of protection of the present invention.

Claims

1. A heavy metal passivation device for Haematococcus pluvialis culture medium based on chelating agents, characterized in that, It includes a buffer tank for collecting culture medium, a power box located below the bottom of the buffer tank, and a stirring drum located inside the buffer tank; the power box is equipped with a motor and is coaxially connected to the stirring drum; the stirring drum divides the inner cavity of the buffer tank into inner and outer chambers, wherein a spiral infusion plate is coaxially provided inside the stirring drum to convect and stir the culture medium inside the buffer tank; The bottom of the buffer tank is provided with a truncated cone-shaped slag collection platform. The cone surface of the slag collection platform has several slag discharge ports that are evenly spaced in a ring. The bottom surface of the slag collection platform is fitted with several movable caps in a ring shape for sealing and opening the slag discharge ports. The top of the buffer tank is provided with several feeding buckets, which are configured as acid and alkali feeding buckets, chelating agent feeding buckets and flocculant feeding buckets. The device also includes a central control unit, which is connected to a heavy metal concentration sensor and a flow sensor located at the inlet of the buffer tank. The central control unit controls the amount of chelating agent released from the chelating agent feeding tank based on the detection information from the heavy metal concentration sensor.

2. The heavy metal passivation device for Haematococcus pluvialis culture medium based on chelating agents according to claim 1, characterized in that: The central control unit is also connected to a pH sensor installed on the inner wall of the buffer tank, and controls the amount of acid and alkali added to the acid and alkali feeding tank according to the preset pH range.

3. The heavy metal passivation device for Haematococcus pluvialis culture medium based on chelating agents according to claim 2, characterized in that: The size of the movable cover is larger than the size of the slag discharge port and is equal to the size of the conical surface between two adjacent slag discharge ports. A linkage frame is slidably engaged between several movable covers. The linkage frame is sleeved and movable with the motor output shaft. A pair of electric push rods are installed in the top of the power box to drive the linkage frame to rise and fall and switch between engaging and disengaging with several movable covers.

4. The heavy metal passivation device for Haematococcus pluvialis culture medium based on chelating agents according to claim 3, characterized in that: A scraper is vertically engaged between the slag accumulation platform and the side wall of the buffer tank, dividing the bottom cavity of the buffer tank into several sedimentation cavities. The scraper is fixedly connected to the top surface of one side of the movable cover by a connecting rod, and the scraper has a structure that is wider at the bottom and narrower at the top.

5. The heavy metal passivation device for Haematococcus pluvialis culture medium based on chelating agents according to claim 4, characterized in that: The movable cover has a locking block in the middle of its concave arc surface. The locking block engages with the outer end of the linkage frame. The linkage frame has a circular ring at its center and several vertical slots on the inner wall of the ring. The output shaft of the motor has several vertical ribs on its outer wall, which are correspondingly engaged with the slots.

6. The heavy metal passivation device for Haematococcus pluvialis culture medium based on chelating agents according to claim 5, characterized in that: A rotating ring is fixedly provided on the bottom surface of the circular ring at the center of the linkage frame. An annular groove is provided on the bottom surface of the rotating ring, and the longitudinal section of the annular groove is a cavity that is wider at the top and narrower at the bottom. A top rod is fixedly connected to the top of the electric push rod, and the top of the top rod is adapted to slide and engage with the annular groove.

7. The heavy metal passivation device for Haematococcus pluvialis culture medium based on chelating agents according to claim 6, characterized in that: The outer wall of the stirring cylinder is provided with a number of stirring rods at equal intervals in a ring. The sides of the stirring rods are provided with a number of liquid-repellent holes. A fixing frame is fixedly provided on the top of the stirring cylinder, and the middle part of the fixing frame is fixedly connected to the top of the spiral infusion plate.

8. The heavy metal passivation device for Haematococcus pluvialis culture medium based on chelating agent according to claim 7, characterized in that: A drain nozzle is installed on the side wall of the buffer tank at the same height as the slag accumulation platform, and a solenoid valve is installed on the drain nozzle; the drain nozzle is connected to a detection tank, and a heavy metal concentration sensor is installed inside the detection tank, which is signal-connected to the central control unit.

9. The heavy metal passivation device for Haematococcus pluvialis culture medium based on chelating agents according to claim 8, characterized in that: A reflux pipeline is provided between the detection tank and the buffer tank. One end of the reflux pipeline is connected to the bottom of the detection tank, and the other end is connected to the top side wall of the buffer tank. A reflux pump and a solenoid valve are provided on the reflux pipeline. The solenoid valve is controlled by the central control unit. When the detection value of the heavy metal concentration sensor in the detection tank exceeds the standard, the central control unit controls the opening of the solenoid valve and starts the reflux pump to pump the liquid in the detection tank back to the buffer tank for further processing.

10. The heavy metal passivation device for Haematococcus pluvialis culture medium based on chelating agents according to claim 9, characterized in that: A solenoid valve is installed on the connecting pipe between the feeding tank and the buffer tank. The valve is controlled to start and stop by the central control unit. The flocculant is added to the feeding tank and forms a precipitate in conjunction with the chelating agent. A liquid level sensor is installed on the inner wall of the buffer tank at a height such as the slag accumulation platform. The central control unit coordinates the start and stop of the solenoid valves on several feeding tanks, drain nozzles and return pipelines based on the signal from the liquid level sensor.

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