Portable multi-pathogen nucleic acid rapid detector based on CRISPR biosensing

Abstract

The invention discloses a portable multi-pathogen nucleic acid rapid detection method and detector based on CRISPR biosensing, and relates to the technical field of nucleic acid detection.The detector comprises a containing box, a bottom plate is fixed in the box, a first supporting seat, a second supporting seat and a computer module are arranged on the bottom plate, the first supporting seat is rotationally connected with a chip mounting assembly, and the chip mounting assembly is rotationally connected with a chip. The chip installation assembly is provided with a micro-fluidic chip, multi-state locking of the chip installation assembly is achieved through the locking piece and the second supporting base, and the computer module is provided with an adjustable display assembly in a matched mode. According to the detection method, multi-point transportation is achieved through the folded and stored detector, samples are processed through the micro-fluidic chip after the detector is unfolded, and nucleic acid amplification, detection and result display are completed in combination with the optical detection and heating module and the computer module. The device is regular in structure, convenient to transport, flexible to operate, stable in detection and suitable for on-site and multi-point nucleic acid detection scenes, and the detection efficiency and applicability are effectively improved.

Description

Technical Field

[0001] This invention relates to the field of nucleic acid detection technology, specifically to a portable rapid nucleic acid detection instrument for multiplex pathogens based on CRISPR biosensors. Background Technology

[0002] Nucleic acid testing, as the gold standard for pathogen detection, plays an irreplaceable role in infectious disease control, clinical diagnosis, and food safety monitoring. With the increasing frequency of global public health emergencies, higher demands are being placed on the speed, portability, and multi-site testing capabilities of nucleic acid testing. Traditional nucleic acid testing procedures typically consist of three core steps: sample pretreatment, nucleic acid extraction, and nucleic acid amplification, all of which must be completed in a specialized central laboratory. Sample pretreatment often uses chemical lysis to release nucleic acids, which is cumbersome and time-consuming. Nucleic acid extraction relies on large equipment such as magnetic bead-based nucleic acid extractors, resulting in high costs. Nucleic acid amplification primarily uses PCR technology, which usually takes more than an hour, and the entire process requires professional technicians, making it difficult to meet the needs of rapid on-site testing and multi-site mobile testing.

[0003] While some existing nucleic acid testing instruments attempt to simplify their structure and reduce their size—for example, the nucleic acid testing instrument disclosed in Chinese invention patent CN215833255U achieves a compact structure by integrating chip mounting components, heating devices, optical detection devices, and robotic arm components—significant drawbacks remain in practical applications. The overall shape of these instruments lacks a regular design, with exposed or scattered functional components, making them susceptible to damage during transportation when multi-point mobile testing is required. They are also inconvenient to carry and difficult to adapt to diverse testing scenarios such as disease control sites, community healthcare, and remote areas. Furthermore, the display components of existing instruments are mostly fixed structures, unable to adjust their angle according to the operating environment, affecting operator observation; the chip mounting components use a single fixing method with insufficient locking stability, potentially loosening during transportation or testing, affecting detection accuracy; simultaneously, the locking components are prone to displacement during transportation, potentially colliding with other components and causing equipment malfunction.

[0004] On the other hand, CRISPR biosensor technology, with its advantages of high specificity and high sensitivity, has been gradually applied in the field of nucleic acid detection. However, there is still room for optimization in its integration with portable detection devices. Existing CRISPR biosensor-based detection devices often fail to fully consider the balance between ease of transport and operational flexibility, resulting in either complex structures and large sizes or simplified functions and insufficient detection stability. Therefore, developing a portable, rapid nucleic acid detection instrument for multiplex pathogens based on CRISPR biosensors that is aesthetically pleasing, easy to transport, flexible in operation, and provides stable detection has become a key requirement for addressing current pain points in nucleic acid detection and improving detection efficiency and coverage. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a portable rapid nucleic acid detection instrument for multiplex pathogens based on CRISPR biosensors, which solves the problems mentioned in the background section.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a portable rapid nucleic acid detection instrument for multiplex pathogens based on CRISPR biosensors, comprising a container, a base plate fixedly disposed within the container, a support base one, a support base two, and a computer module on the base plate, a chip mounting assembly for mounting a microfluidic chip rotatably connected to the support base one, a pluggable locking element between the support base one and the chip mounting assembly, the support base two locking the chip mounting assembly in a horizontal position, and an adjustable-angle display component on the computer module; the chip mounting assembly enables the positioning and reaction control of the microfluidic chip, and the computer module and display component are combined to process and display nucleic acid detection data; during the detection process, the instrument can be easily transported by folding the chip mounting assembly and display component.

[0007] Optionally, the container includes a container body and a rotatably connected lid. The lid and the container body are fixed in a closed state by two threaded bolts. A base plate is fixedly connected to the bottom surface of the container body, and the base plate provides an installation reference surface for each component.

[0008] Optionally, the second support includes a seat body fixed to the base plate. The seat body is slidably connected to two sliding rods. One end of each sliding rod is fixed to a baffle, and the other end is fixed to a connecting block. A spring is fixed between the baffle and the seat body. The connecting block is fixed to two locking rods. Under normal conditions, the spring pushes the connecting block to be tightly pressed against the seat body.

[0009] Optionally, the chip mounting assembly includes a mounting plate with two locking holes at the upper end that are adapted to the locking rod. When the locking rod is inserted into the locking hole, the mounting plate is fixed in a horizontal position. A locking plate is fixed at the lower end of the mounting plate. The locking plate cooperates with the support base to fix the mounting plate in a vertical state.

[0010] Optionally, a placement box is fixed on the mounting plate, the microfluidic chip is slidably inserted into the placement box, the edge of the microfluidic chip is provided with several pistons, the placement box is provided with through holes corresponding to the positions of the pistons, the mounting plate is fixedly connected to a corner plate, and the corner plate is fixed with several electric telescopic rods corresponding to the pistons one by one. The electric telescopic rods can pass through the through holes to push the pistons to move.

[0011] Optionally, the optical detection and heating module is fixed together with the placement box and the mounting plate, and the microfluidic chip is located between the two components of the optical detection and heating module. The optical detection and heating module can heat and perform optical detection on the amplification part of the microfluidic chip.

[0012] Optionally, the locking component includes a connecting strip, which fixes three locking rods and the handle. When the locking plate is attached to the support base, the locking rods slide into the locking plate and the support base. The connecting strip has two mounting slots, and magnets are fixed in the mounting slots.

[0013] Optionally, the display component includes a touch screen rotatably connected to the computer module, with connecting rods rotatably connected to both sides of the touch screen, two guide rails symmetrically fixed to the computer module, sliders slidably connected to the guide rails, and the end of the connecting rod away from the touch screen rotatably connected to the slider.

[0014] Optionally, the guide rail is provided with a plurality of positioning holes evenly distributed, and the slider is provided with a second locking hole. When the positioning hole and the second locking hole are aligned, a locking pin is inserted inside to fix the position of the slider, thereby locking the tilt angle of the touch screen.

[0015] Optionally, a magnetic ring is fixed on the locking pin, and the magnetic ring can be attracted to the surface of the guide rail to prevent the locking pin from coming off the positioning hole and the locking hole during transportation.

[0016] This invention provides a portable rapid nucleic acid detection instrument for multiplex pathogens based on CRISPR biosensor, which has the following beneficial effects: This portable multipathogen nucleic acid rapid detection instrument based on CRISPR biosensors integrates the chip mounting component, computer module, display component, and other functional components into a neatly designed container, resulting in a streamlined structure that greatly facilitates transportation and carrying between disease control centers, communities, and remote areas. Furthermore, the chip mounting component is rotatable and foldable, and the display component is adjustable for storage, effectively reducing the device's size and further enhancing transport convenience. The container is secured to the lid and body with bolts, and the internal locking mechanism uses magnets and locking pins to firmly attach the components, preventing accidental movement or collisions during transport, ensuring the safety of the equipment and reducing the risk of damage. Secondly, the chip mounting assembly rotates via support base one, allowing for flexible switching between horizontal and vertical locking states: the horizontal state facilitates the installation and removal of the microfluidic chip, while the vertical state facilitates observation and operation during testing. The dual locking design of the locking element and support base two ensures stable fixation in different states, making operation simple and efficient. The display assembly, through the cooperation of linkages, sliders, and guide rails, allows for flexible adjustment of the touch screen's tilt angle. Operators can adjust to the optimal viewing angle based on ambient light, operating posture, and other practical factors, enhancing the user experience. The locking pin and positioning hole work together to quickly fix the adjusted position of the display assembly, preventing angle shift during use. Furthermore, the second support base adopts a spring-driven locking rod design. When the chip mounting assembly is in a horizontal state, the spring force ensures that the locking rod is tightly inserted into the locking hole, achieving a stable lock and preventing the chip mounting assembly from loosening during the detection process. The magnetic block of the locking component allows the locking rod to be tightly attracted to the first support base and the locking plate, further improving the fixation reliability of the chip mounting assembly in the vertical state and providing a guarantee for the stable response of the microfluidic chip. The precise alignment design of the optical detection and heating module with the microfluidic chip, combined with the stable drive of the piston by the electric telescopic rod, ensures the orderly progress of sample processing, nucleic acid amplification, and optical detection, improving the accuracy and repeatability of the detection results. In addition, the equipment has a simple overall structure, and can be deployed, chip installed, and tested without the need for professional technicians. It is suitable for various testing scenarios such as community healthcare, disease control sites, and remote areas, effectively expanding the coverage of nucleic acid testing. The closed design of the container can protect the internal components from dust, moisture, and collisions, extending the service life of the equipment. The connection method of each component is simple and reliable, and maintenance is convenient, reducing the cost of use. Meanwhile, this invention enables rapid detection of multiple pathogens. Combining the high specificity and high sensitivity of CRISPR biosensor technology, it can quickly and accurately identify the nucleic acids of different pathogens, providing timely and effective data support for infectious disease prevention and control, clinical diagnosis, etc., and has important practical application value. Attached Figure Description

[0017] Figure 1 This is an overall schematic diagram of the present invention; Figure 2 This is a schematic diagram of the structure of the container box after it is opened; Figure 3 This is a schematic diagram of the structure of the container of the present invention after it is fully closed; Figure 4 This is a schematic diagram of the structure of the second support base of the present invention; Figure 5 This is a schematic diagram of the chip mounting assembly of the present invention. Figure 1 ; Figure 6 This is a schematic diagram of the chip mounting assembly of the present invention. Figure 2 ; Figure 7 This is a schematic diagram of the locking component of the present invention; Figure 8 This is a schematic diagram of the display mechanism of the present invention.

[0018] In the diagram: 1. Holding box; 101. Box body; 102. Box lid; 103. Bolt; 2. Base plate; 3. Support base one; 4. Support base two; 401. Base body; 402. Sliding rod; 403. Baffle; 404. Spring; 405. Connecting block; 406. Locking rod one; 5. Chip mounting assembly; 501. Mounting plate; 502. Locking hole one; 503. Placement box; 504. Connecting corner plate; 505. Electric telescopic rod; 506. Optical inspection... Measurement and heating module; 507, locking plate; 6, microfluidic chip; 601, piston; 7, locking component; 701, connecting bar; 702, locking lever two; 703, handle; 704, mounting slot; 705, magnet block; 8, computer module; 9, display assembly; 901, touch screen; 902, connecting rod; 903, guide rail; 904, slider; 905, positioning hole; 906, locking hole two; 907, locking pin; 908, magnet ring. Detailed Implementation

[0019] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0020] In the description of this invention, unless otherwise stated, "a plurality of" means two or more; the terms "upper," "lower," "left," "right," "inner," "outer," "front end," "rear end," "head," "tail," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the invention. Furthermore, the terms "first," "second," "third," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0021] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0022] Please see Figures 1 to 8 This invention provides a technical solution: a portable rapid nucleic acid detection instrument for multiplex pathogens based on CRISPR biosensor, comprising a container 1, a base plate 2 fixedly disposed inside the container 1, a support base 3, a support base 4, and a computer module 8 on the base plate 2, a chip mounting assembly 5 for mounting a microfluidic chip 6 rotatably connected to the support base 3, a pluggable locking element 7 between the support base 3 and the chip mounting assembly 5, the support base 4 locking the chip mounting assembly 5 in a horizontal state, and an adjustable display assembly 9 on the computer module 8; the positioning and reaction control of the microfluidic chip 6 are achieved through the chip mounting assembly 5, and the processing and display of nucleic acid detection data are completed by combining the computer module 8 and the display assembly 9; during the detection process, the instrument can be conveniently transported by folding the chip mounting assembly 5 and the display assembly 9.

[0023] In this embodiment, the container 1 includes a container body 101 and a container cover 102 that is rotatably connected. The container cover 102 and the container body 101 are fixedly closed by two bolts 103 that are threadedly connected. The bottom surface of the container body 101 is fixedly connected to a base plate 2, which provides an installation reference surface for each component.

[0024] In this embodiment, the second support 4 includes a seat body 401 fixed to the base plate 2. The seat body 401 is slidably connected to two slide rods 402. One end of the two slide rods 402 is fixed to a baffle 403, and the other end is fixed to a connecting block 405. A spring 404 is fixed between the baffle 403 and the seat body 401. The connecting block 405 fixes two locking rods 406. Under normal conditions, the spring 404 pushes the connecting block 405 to be tightly pressed against the seat body 401.

[0025] In this embodiment, the chip mounting assembly 5 includes a mounting plate 501. The upper end of the mounting plate 501 has two locking holes 502 that are adapted to the locking rod 406. When the locking rod 406 is inserted into the locking hole 502, the mounting plate 501 is fixed in a horizontal position. The lower end of the mounting plate 501 is fixed with a locking plate 507. The locking plate 507 cooperates with the support base 3 to fix the mounting plate 501 in a vertical state.

[0026] In this embodiment, a placement box 503 is fixed on the mounting plate 501, and the microfluidic chip 6 is slidably inserted into the placement box 503. The edge of the microfluidic chip 6 is provided with several pistons 601. The placement box 503 has through holes corresponding to the positions of the pistons 601. The mounting plate 501 is fixedly connected to a corner plate 504. The corner plate 504 is fixed with several electric telescopic rods 505 corresponding to the pistons 601. The electric telescopic rods 505 can pass through the through holes to push the pistons 601 to move.

[0027] In this embodiment, the optical detection and heating module 506 is fixed together on the placement box 503 and the mounting plate 501. The microfluidic chip 6 is located between the two components of the optical detection and heating module 506. The optical detection and heating module 506 can heat and perform optical detection on the amplification part of the microfluidic chip 6.

[0028] In this embodiment, the locking component 7 includes a connecting strip 701, which fixes three locking rods 702 and a handle 703. When the locking plate 507 is attached to the support base 3, the locking rods 702 slide into the locking plate 507 and the support base 3. The connecting strip 701 has two mounting grooves 704, and a magnet block 705 is fixed in the mounting groove 704.

[0029] In this embodiment, the display component 9 includes a touch screen 901 rotatably connected to the computer module 8. Both sides of the touch screen 901 are rotatably connected to connecting rods 902. The computer module 8 is symmetrically fixed with two guide rails 903. The guide rails 903 are slidably connected to sliders 904. The end of the connecting rod 902 away from the touch screen 901 is rotatably connected to the slider 904.

[0030] In this embodiment, a plurality of positioning holes 905 are evenly provided on the guide rail 903, and a second locking hole 906 is provided on the slider 904. When the positioning hole 905 and the second locking hole 906 are aligned, a locking pin 907 is inserted inside to fix the position of the slider 904, thereby locking the tilt angle of the touch screen 901.

[0031] In this embodiment, a magnetic ring 908 is fixed on the locking pin 907. The magnetic ring 908 can be attracted to the surface of the guide rail 903 to prevent the locking pin 907 from falling off the positioning hole 905 and the second locking hole 906 during transportation. Example

[0032] This embodiment provides a portable rapid nucleic acid detection instrument for multiplex pathogens based on CRISPR biosensor, the specific structure of which is as follows: The container 1 includes a container body 101 and a container cover 102. The container cover 102 and the container body 101 are fixed in a closed state by two bolts 103. The bottom plate 2 is fixedly connected to the inner bottom surface of the container body 101. The base plate 2 is fixed with support base 1 3, support base 2 4 and computer module 8. Support base 1 3 is rotatably connected to chip mounting assembly 5. Chip mounting assembly 5 includes mounting plate 501. The upper end of mounting plate 501 has two locking holes 502 and the lower end is fixed with locking plate 507. The mounting plate 501 is fixed with placement box 503. Microfluidic chip 6 is slidably inserted into placement box 503. Several pistons 601 are provided on the edge of microfluidic chip 6. Placement box 503 has through holes corresponding to pistons 601. Mounting plate 501 is fixed with angle plate 504. The angle plate 504 is fixed with several electric telescopic rods 505 corresponding to pistons 601. Optical detection and heating module 506 is fixed on placement box 503 and mounting plate 501. Microfluidic chip 6 is located between two components of optical detection and heating module 506.

[0033] Support base 2 4 includes a base body 401, with two sliding rods 402 slidably connected to the base body 401. One end of each sliding rod 402 is fixed to a baffle 403, and the other end is fixed to a connecting block 405. A spring 404 is fixed between the baffle 403 and the base body 401. The connecting block 405 fixes two locking rods 406. Locking component 7 includes a connecting strip 701, which fixes three locking rods 702 and a handle 703. The connecting strip 701 has two mounting slots 704, and a magnet block 705 is fixed in each mounting slot 704.

[0034] Display component 9 includes a touch screen 901, with connecting rods 902 rotatably connected to both sides of the touch screen 901. Computer module 8 fixes two guide rails 903, with sliders 904 slidably connected to the guide rails 903. The connecting rods 902 are rotatably connected to the sliders 904. The guide rails 903 have several positioning holes 905, and the sliders 904 have a second locking hole 906. Locking pins 907 are inserted into the positioning holes 905 and the second locking hole 906, and the locking pins 907 fix a magnetic ring 908.

[0035] The working principle is as follows: First, rotate the chip mounting assembly 5 to a horizontal position. The spring 404 of the support base 2 4 pushes the connecting block 405, causing the locking rod 1 406 to insert into the locking hole 1 502 of the mounting plate 501, thus achieving horizontal locking of the chip mounting assembly 5. Adjust the slider 904 of the display assembly 9 so that the touch screen 901 is folded close to the surface of the computer module 8, and insert the locking pin 907 to fix the slider 904. Insert the locking rod 2 702 of the locking component 7 into the support base 1 3 and the locking plate 507. The magnet block 705 attracts and fixes it, preventing the locking component 7 from shifting during transportation. Close the box cover 102 and tighten the bolts 103 to fix the box body 101 and the box cover 102. At this time, the detector is a regular box-shaped structure and can be transported safely. After transportation to the testing location, unscrew bolt 103 and open box cover 102; pull out locking pin 907, push slider 904 to slide along guide rail 903, drive touch screen 901 to rotate to a suitable angle through connecting rod 902, align positioning hole 905 and locking hole 2 906, insert locking pin 907, magnet ring 908 adsorbs guide rail 903 to fix; pull baffle 403 of support base 2 4, drive slide rod 402 and connecting block 405 to move, so that locking rod 1 406 disengages from locking hole 1 502, rotate chip mounting assembly 5 to vertical position, insert locking rod 2 702 of locking component 7 into support base 1 3 and locking plate 507, magnet block 705 adsorbs and fixes, realize vertical locking of chip mounting assembly 5; The microfluidic chip 6, pre-injected with the sample, is inserted into the placement box 503. The computer module 8 sends a control signal to drive the electric telescopic rod 505 to push the piston 601 of the microfluidic chip 6 in a preset sequence, realizing the pre-processing procedures such as sample lysis and nucleic acid purification. After the pre-processing is completed, the optical detection and heating module 506 heats the amplification site of the microfluidic chip 6 to provide a suitable temperature environment for nucleic acid amplification and accelerate the CRISPR biosensor-mediated nucleic acid amplification reaction. After amplification, the optical detection and heating module 506 emits light of a specific wavelength to irradiate the amplification product, exciting the product to emit fluorescence. The optical detection component receives the fluorescence signal and transmits it to the computer module 8. The computer module 8 analyzes and processes the fluorescence signal, determines the type of pathogen by comparing it with a preset standard, and displays the detection result on the touch screen 901 in real time. After the test is completed, fold and store each component in reverse order of the above steps, close container 1, and then transport it to the next test point. Example

[0036] This embodiment is basically the same as the first embodiment in structure, except that the angle adjustment structure of the display component 9 is optimized, as follows: Display component 9 includes a touch screen 901. The bottom of the touch screen 901 is rotatably connected to the computer module 8 via a damping pivot. Hydraulic telescopic rods are respectively installed on both sides of the touch screen 901 and the computer module 8, with their ends rotatably connected to the side wall of the touch screen 901 and the surface of the computer module 8, respectively. By extending and retracting the hydraulic telescopic rods, the touch screen 901 rotates around the damping pivot, achieving angle adjustment. Once adjusted to the desired position, the self-locking function of the hydraulic telescopic rods secures the touch screen 901, eliminating the need for an additional locking pin 907. This structure offers easier operation and smoother angle adjustment, making it suitable for scenarios requiring high ease of operation.

[0037] The working principle is as follows: The core working principle of the storage, transportation, and testing stages in this embodiment is the same as that in Embodiment 1, with the only difference being the angle adjustment principle of component 9: During the angle adjustment phase of component 9: When unfolding the testing instrument, activate the control switch of the hydraulic telescopic rod. The hydraulic telescopic rod extends and retracts, causing the touch screen 901 to rotate around the damping shaft. After the operator observes and determines the appropriate angle, the control switch is turned off. The hydraulic telescopic rod then uses its self-locking function to fix its length, thereby fixing the position of the touch screen 901. After the test is completed, activate the hydraulic telescopic rod to extend and retract in the reverse direction, causing the touch screen 901 to fold close to the surface of the computer module 8, completing the storage. This process does not require manual insertion and removal of the locking pin 907, making operation more convenient and angle adjustment more accurate, suitable for testing scenarios requiring frequent angle adjustments.

[0038] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A portable rapid nucleic acid detection instrument for multiplex pathogens based on CRISPR biosensor, comprising a container (1), characterized in that, The container (1) is fixedly provided with a base plate (2). The base plate (2) is provided with a support seat one (3), a support seat two (4) and a computer module (8). The support seat one (3) is rotatably connected to a chip mounting assembly (5) for mounting a microfluidic chip (6). A pluggable locking element (7) is provided between the support seat one (3) and the chip mounting assembly (5). The support seat two (4) can lock the chip mounting assembly (5) in a horizontal state. The computer module (8) is provided with an adjustable angle display assembly (9). The positioning and reaction control of the microfluidic chip (6) are realized through the chip mounting assembly (5). The processing and display of nucleic acid detection data are completed by combining the computer module (8) and the display assembly (9). During the detection process, the detector can be portable and transported by folding the chip mounting assembly (5) and the display assembly (9).

2. The portable rapid nucleic acid detection method and instrument for multiplex pathogens based on CRISPR biosensor as described in claim 1, characterized in that, The container (1) includes a container body (101) and a container cover (102) that is rotatably connected. The container cover (102) and the container body (101) are fixed in a closed state by two bolts (103) that are threadedly connected. The bottom surface of the container body (101) is fixedly connected to a base plate (2), which provides an installation reference surface for each component.

3. The portable rapid nucleic acid detection method and instrument for multiplex pathogens based on CRISPR biosensor as described in claim 1, characterized in that, The second support (4) includes a seat (401) fixed to the base plate (2). The seat (401) is slidably connected to two slide rods (402). One end of the two slide rods (402) is fixed to a baffle (403), and the other end is fixed to a connecting block (405). A spring (404) is fixed between the baffle (403) and the seat (401). The connecting block (405) is fixed to two locking rods (406). Under normal conditions, the spring (404) pushes the connecting block (405) to be tightly pressed against the seat (401).

4. The portable rapid nucleic acid detection method and instrument for multiplex pathogens based on CRISPR biosensor according to claim 3, characterized in that, The chip mounting assembly (5) includes a mounting plate (501). The upper end of the mounting plate (501) is provided with two locking holes (502) that are adapted to the locking rod (406). When the locking rod (406) is inserted into the locking hole (502), the mounting plate (501) is fixed in a horizontal position. The lower end of the mounting plate (501) is fixed with a locking plate (507). The locking plate (507) cooperates with the support base (3) to fix the mounting plate (501) in a vertical state.

5. The portable rapid nucleic acid detection method and instrument for multiplex pathogens based on CRISPR biosensor according to claim 4, characterized in that, The mounting plate (501) is fixed with a placement box (503), and the microfluidic chip (6) is slidably inserted into the placement box (503). The edge of the microfluidic chip (6) is provided with several pistons (601). The placement box (503) is provided with through holes corresponding to the positions of the pistons (601). The mounting plate (501) is fixed with a connecting angle plate (504). The connecting angle plate (504) is fixed with several electric telescopic rods (505) corresponding one-to-one with the pistons (601). The electric telescopic rods (505) can pass through the through holes to push the pistons (601) to move.

6. The portable rapid nucleic acid detection method and instrument for multiplex pathogens based on CRISPR biosensor according to claim 5, characterized in that, The optical detection and heating module (506) is fixed together on the placement box (503) and the mounting plate (501). The microfluidic chip (6) is located between the two components of the optical detection and heating module (506). The optical detection and heating module (506) can heat and perform optical detection on the amplification part of the microfluidic chip (6).

7. The portable rapid nucleic acid detection method and instrument for multiplex pathogens based on CRISPR biosensor according to claim 1, characterized in that, The locking component (7) includes a connecting strip (701), which fixes three locking rods (702) and a handle (703). When the locking plate (507) is in contact with the support base (3), the locking rods (702) slide into the locking plate (507) and the support base (3). The connecting strip (701) has two mounting slots (704), and a magnet block (705) is fixed in the mounting slot (704).

8. The portable rapid nucleic acid detection method and instrument for multiplex pathogens based on CRISPR biosensor according to claim 1, characterized in that, The display component (9) includes a touch screen (901) rotatably connected to the computer module (8). Both sides of the touch screen (901) are rotatably connected to connecting rods (902). The computer module (8) is symmetrically fixed with two guide rails (903). The guide rails (903) are slidably connected to sliders (904). The end of the connecting rod (902) away from the touch screen (901) is rotatably connected to the slider (904).

9. The portable rapid nucleic acid detection method and instrument for multiplex pathogens based on CRISPR biosensor as described in claim 8, characterized in that, The guide rail (903) is provided with a plurality of positioning holes (905) evenly distributed. The slider (904) is provided with a second locking hole (906). When the positioning hole (905) and the second locking hole (906) are aligned, a locking pin (907) is inserted inside to fix the position of the slider (904) and thereby lock the tilt angle of the touch screen (901).

10. The portable rapid nucleic acid detection method and instrument for multiplex pathogens based on CRISPR biosensor according to claim 9, characterized in that, A magnet ring (908) is fixed on the locking pin (907). The magnet ring (908) can be attracted to the surface of the guide rail (903) to prevent the locking pin (907) from coming off the positioning hole (905) and the second locking hole (906) during transportation.

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