Device and method for detecting active substances of browned Chinese wolfberry fruits

Abstract

The invention relates to the field of microbiological detection, in particular to a browning wolfberry active substance detection device, a plurality of flexible detection tubes are arranged in the middle of a machine body, the outer wall of each flexible detection tube is in extrusion contact with an eccentric wheel capable of vertically rolling, and the eccentric wheels roll to drive feeding balls mounted in inner cavities of the flexible detection tubes to vertically move; and the feeding ball and the flexible detection tube are internally provided with a glass tube for inputting wolfberry liquid to form a butt joint liquid supplementing mechanism. According to the device, the eccentric wheel wriggling mechanism can drive the feeding ball to perform dynamic feeding while enabling the flexible detection tube to simulate intestinal movement, the feeding ball can perform automatic supply with the glass tube filled with wolfberry liquid while moving up and down, and the glass tube is linked to perform material supply counting, so that the overall reaction efficiency of the device is improved, and the labor intensity of workers is reduced. And the utilization efficiency among the microbial components can be intuitively represented.

Description

Technical Field

[0001] This invention relates to the field of microbial detection technology, and in particular to a device and method for detecting active substances in browned wolfberry. Background Technology

[0002] When wolfberries undergo the Maillard reaction under specific conditions, they produce a substance called melanoidin, which enhances the antioxidant and anti-aging capabilities of the final product. Therefore, existing technologies conduct activity testing on browned wolfberries to analyze the relationship between browning products and specific microbial media.

[0003] Currently, the detection devices and methods for active substances in browned wolfberries generally involve mixing wolfberry liquid after Maillard reaction with a specific microbial medium, and then using a detection instrument to detect the reaction products and other data.

[0004] Especially for microbial detection steps, the traditional method involves mixing wolfberry juice and probiotics in a test tube using reaction equipment and then analyzing the prebiotic activity by measuring the turbidity. However, traditional methods often involve directly mixing the two liquids and letting them stand. Some instruments can perform rotational stirring, but overall, this type of structure lacks good physical dynamic assistance. The contact between active substances and microorganisms relies solely on molecular thermal motion, resulting in slow renewal of the reaction interface. At the same time, the one-time mixing mode leads to large fluctuations in the concentration of biomolecules in the system, making it difficult to intuitively understand the reaction results between wolfberry juice and the microbial medium, and making it difficult to obtain stable and uniform data, thus affecting the detection efficiency. Summary of the Invention

[0005] The purpose of this invention is to address the shortcomings of existing technologies by proposing a detection device and method for active substances in browned wolfberries.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: A device for detecting active substances in browned wolfberries includes a body with multiple flexible detection tubes in the middle. Each flexible detection tube has an eccentric wheel that can roll vertically pressed against its outer wall. The eccentric wheel rolls to drive a feeding ball installed in the inner cavity of the flexible detection tube to move vertically. The feeding ball and a glass tube for inputting wolfberry liquid inside the flexible detection tube form a docking and replenishment mechanism. The feeding chamber inside the feeding ball is equipped with a float rod. The top of the float rod is equipped with a magnetic trigger plate and extends into the flow port at the top of the feeding ball for liquid input. The float rod rises and falls synchronously with the liquid level inside the feeding chamber and drives the magnetic trigger plate to lock and release the plug-in nozzle that is movably sleeved on the outer wall of the flow port. The glass tube is fed by a feeding mechanism. The bottom of the glass tube is provided with an interface that is a connector to the spigot. The glass tube and the spigot are connected by a corrugated pipe and multiple flip plates. When the liquid level in the glass tube is low, the magnetic floating plate inside the tube will descend and attract the flip plates to tilt inward to release the spigot. When the spigot connects with the feeding ball, the counting mechanism is activated to count.

[0007] Preferably, toothed support rods are provided on both sides of the flexible detection tube to provide support. A feeding mechanism for introducing browned wolfberry liquid is provided above the flexible detection tube. The feeding mechanism is connected to the lifting mechanism inside the machine body to realize lifting operation. A laser turbidity sensor is installed at the bottom of the flexible detection tube and is electrically connected to the machine body.

[0008] Preferably, a telescopic rod is provided between the flexible detection tubes, and a support frame is provided at the top of the telescopic rod. Multiple pairs of eccentric wheels are rotatably installed on the outer side of the support frame. The eccentric wheels are in contact with the outer wall of the flexible detection tube. The side wall of the eccentric wheel is provided with a toothed groove and engages with the toothed support rod.

[0009] Preferably, the upper end of the flexible detection tube is fitted with a glass tube, and a connecting nozzle is detachably installed on the upper part of the glass tube. The connecting nozzle is used to connect to the output end of the feeding mechanism to achieve feeding connection. A piston rod is vertically arranged in the center of the glass tube. A piston is installed at the bottom of the piston rod. The piston and the bottom chamber of the glass tube together form a sealed chamber for containing wolfberry liquid. A laser level gauge is installed on one side of the middle part of the piston. A lifting adjustment plate is rotatably installed on the upper part of the piston rod. The outer wall of the lifting adjustment plate is screwed to the internal thread of the upper middle part of the flexible detection tube.

[0010] Preferably, the bottom of the glass tube is flexibly connected to the insertion interface via a corrugated pipe. A magnetic floating plate is vertically and movably mounted inside the glass tube. The magnetic floating plate can slide vertically with the liquid level. Magnetic structures are provided on both sides of the magnetic floating plate. Multiple flip plates are rotatably mounted at the bottom of the glass tube. The flip plates and the magnetic floating plate are magnetically attracted to each other. Under natural working conditions, the flip plates are in a vertical position and abut against the bottom insertion interface to form a stable mechanical support structure. A torsion spring is installed at the rotatable connection between the flip plates and the glass tube.

[0011] Preferably, the counting mechanism includes a movable plate movably sleeved on the outer wall of the insertion interface. A vertically extending rod is connected to one side of the movable plate. The rod extends upward to the counting disk inside the flexible detection tube 3. The counting disk is installed on the upper side of the flexible detection tube. A multi-layer stepping staggered toothed disk is installed at the bottom of the counting disk. Each stepping staggered toothed disk is rotatably installed inside the flexible detection tube via a shaft. A coil spring is provided at the rotatable connection. The two layers of stepping staggered toothed disks adopt an staggered tooth design, and multiple wedge-shaped tooth blocks are distributed on their outer edges.

[0012] Preferably, the outer wall of the feeding ball is provided with an auxiliary plate, and the bottom side of the feeding ball is provided with a feeding cavity for containing wolfberry liquid. The outer wall of the feeding ball has multiple micro-holes for liquid to seep out. A float rod is vertically provided in the middle of the feeding cavity. A float is connected to the bottom end of the float rod, and the rod body extends upward into the inside of the feeding ball and is provided with a magnetic trigger plate at the top. The magnetic trigger plate can be vertically slidably arranged in the flow port. A plug is vertically sleeved on the outer wall of the flow port. The plug is embedded in the top of the feeding ball in the natural state. The plug and the plug interface are plug-in hooks to each other, and the outer wall of the plug is elastically connected to the inside of the feeding ball by a spring.

[0013] Preferably, the side wall of the flow port is provided with a ball groove, and a spring-loaded locking ball is installed in the ball groove. The spring-loaded locking ball and the magnetic trigger plate are magnetically attracted to each other. The spring-loaded locking ball is constrained in the ball groove and exposes half of the ball. In its natural state, the spring-loaded locking ball is pushed by the spring and inserted into the slot in the inner wall of the connector to complete the concave-convex locking.

[0014] Preferably, a rubber plug is installed in the middle of the flow port. When the insertion nozzle is inserted into the feeding ball, the insertion nozzle and the rubber plug are squeezed and contacted to achieve sealing.

[0015] A detection method for an active substance detection device in browned wolfberry includes the following steps: S1. Inject a preset dose of probiotic liquid into the flexible detection tube and introduce mixed gas to maintain an anaerobic microenvironment. At the same time, replenish the glass tube with wolfberry extract through the feeding mechanism. S2. Start the eccentric wheel to rotate and squeeze the tube body, simulate the physiological peristalsis of the intestine to generate physical power, and simultaneously drive the feeding ball inside the tube to slide up and down along the inner wall of the tube to feed the bacteria. Use a laser turbidity sensor to monitor the changes in the absorbance of the bacterial community in real time. S3. When the liquid level in the feeding ball drops, the magnetic attraction mechanism is triggered to release the spout, which then connects with the spout at the bottom of the glass tube during the upward stroke of the feeding ball to replenish the wolfberry liquid. S4. When the liquid in the glass tube is exhausted, the flip plate is deflected and the limit is released. The counting disk is driven to rotate and count by the vertical rod linkage stepping interlocking tooth disk.

[0016] The beneficial effects of this invention are as follows: In this invention, the eccentric wheel peristaltic mechanism enables the flexible detection tube to simulate intestinal movement while driving the feeding ball to dynamically feed. As the feeding ball moves up and down, it automatically replenishes the glass tube containing wolfberry liquid and is linked with the glass tube to count the replenishment. This not only improves the overall reaction efficiency of the device, but also allows for a direct representation of the utilization efficiency between microbial components. Attached Figure Description

[0017] Figure 1This is a schematic diagram of the overall structure of a browning wolfberry active substance detection device proposed in this invention; Figure 2 This is a schematic diagram of the flexible detection tube installation structure proposed in this invention; Figure 3 This is a schematic diagram of the eccentric wheel mounting structure proposed in this invention; Figure 4 This is a schematic diagram of the flexible detection tube and eccentric wheel structure proposed in this invention; Figure 5 This is a cross-sectional view of the internal structure of the flexible detection tube proposed in this invention; Figure 6 This is a partial schematic diagram of the internal structure of the flexible detection tube proposed in this invention; Figure 7 This is a schematic diagram of the external structure of the counting disk proposed in this invention; Figure 8 This is a schematic diagram of the magnetic floating plate structure proposed in this invention; Figure 9 This is a schematic diagram of the feeding ball structure proposed in this invention; Figure 10 This is a partial schematic diagram of the internal structure of the feeding ball proposed in this invention; Figure 11 This is a partial structural diagram of the connector proposed in this invention; Figure 12 This is a schematic diagram of the protruding connector structure proposed in this invention.

[0018] In the diagram: 1. Machine body; 2. Feeding mechanism; 3. Flexible detection tube; 31. Toothed support rod; 4. Eccentric wheel; 5. Telescopic rod; 51. Support frame; 6. Magnetic floating plate; 7. Movable plate; 71. Vertical rod; 8. Laser turbidity sensor; 9. Connecting nozzle; 10. Counting disc; 11. Feeding ball; 111. Auxiliary plate; 112. Feeding chamber; 12. Glass tube; 121. Insertion interface; 13. Piston rod; 131. Lifting adjustment disc; 132. Laser level gauge; 14. Tilting plate; 15. Corrugated pipe; 16. Float rod; 161. Magnetic trigger disc; 17. Insertion nozzle; 171. Slot; 18. Rubber stopper; 19. Spring; 20. Flow port; 21. Rebound locking ball; 22. Stepping staggered toothed disc; 23. Coil spring. Detailed Implementation

[0019] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0020] Reference Figure 1-4A device for detecting active substances in browned wolfberry includes a body 1. Multiple flexible detection tubes 3 are installed in the middle of the body 1 to form multiple reaction units. Rigid support tubes are provided at the upper and lower ends of the flexible detection tubes 3 to achieve a support effect. The upper and lower support tubes are rigidly connected and axially positioned by a pair of toothed support rods 31.

[0021] The inner cavity of the flexible detection tube 3 is equipped with a reaction chamber specifically for filling probiotic liquid. Above the flexible detection tube 3, there is a feeding mechanism 2 for introducing browning wolfberry liquid. The feeding mechanism 2 is connected to the lifting mechanism inside the machine body 1 to realize the lifting operation. The lifting mechanism can be any one of the screw lifting mechanism, pneumatic lifting mechanism or hydraulic lifting mechanism. This is a conventional configuration in the field and will not be described in detail.

[0022] Telescopic rods 5 are arranged between these flexible detection tubes 3. The telescopic rods 5 can be either electric or pneumatic. A support frame 51 is provided at the end of the telescopic rod 5. Multiple pairs of eccentric wheels 4 are rotatably installed on the outer side of the support frame 51. The sidewalls of the eccentric wheels 4 are provided with tooth grooves, and an eccentric wheel body structure is provided in the middle.

[0023] Adjacent eccentric wheels 4 are mirror-distributed and firmly clamp the flexible detection tube 3. The wheel surfaces make contact with the side walls of the flexible detection tube 3 through compression. Since the flexible detection tube 3 is made of highly elastic flexible material, it can effectively respond to the compressive load of the eccentric wheels 4. At the same time, the tooth grooves on the outer wall of the eccentric wheel 4 will form a meshing pair with the tooth grooves on the side wall of the toothed support rod 31. As the telescopic rod 5 drives the support frame 51 to make vertical displacement, the meshing action drives the eccentric wheel 4 to generate rotation. Thus, its eccentric structure is used to implement periodic asymmetric compression on the tube body, simulating the physiological peristalsis of the intestine, achieving effective physical power assistance and improving reaction efficiency.

[0024] Furthermore, an input gas pipe and an output gas pipe are respectively provided at the top and bottom of one side of the flexible detection tube 3, so as to realize the input of anaerobic and microaerophilic gases and the timely discharge of metabolic waste gas. In addition, a water pipe interface for replenishing probiotic liquid is provided on one side of the top of the flexible detection tube 3. The water pipe interface is connected to the reaction chamber of the flexible detection tube 3. The input ends of the above-mentioned gas pipe and water pipe are connected to the internal supply mechanism of the body 1. The body 1 has an integrated supply mechanism for supplying gas and liquid. This mechanism is a conventional configuration in the art. The above-mentioned specific pipeline connection methods are common knowledge to those skilled in the art and will not be explained further. Next, a laser turbidity sensor 8 is installed at the bottom of the flexible detection tube 3, with the sensing end facing the inside of the flexible detection tube 3. The laser turbidity sensor 8 realizes quantitative evaluation of the degree of reaction and biological activity by monitoring the changes in absorbance of the bacterial community in the cavity in real time.

[0025] Reference Figure 5-8 A glass tube 12 is installed at the upper end of the flexible detection tube 3. The glass tube 12 has a chamber for inputting wolfberry liquid. At the same time, a connecting nozzle 9 is detachably installed on the upper part of the flexible detection tube 3. The connecting nozzle 9 is used to connect to the output end of the feeding mechanism 2 to realize the feeding connection. The connecting nozzle 9 is connected to the glass tube 12, and the wolfberry liquid is input through this.

[0026] A piston rod 13 is vertically installed at the center of the glass tube 12. A piston is installed at the bottom of the piston rod 13. A laser level gauge 132 is installed on one side of the middle of the piston to detect the liquid level below without contact. The piston and the bottom chamber of the glass tube 12 together form a sealed chamber for containing wolfberry liquid. A lifting adjustment plate 131 is rotatably installed on the upper part of the piston rod 13. The outer wall of the lifting adjustment plate 131 is screwed to the internal thread at the middle of the upper end of the flexible detection tube 3. By rotating the lifting adjustment plate 131, the piston rod 13 can be vertically displaced as a whole by means of the thread engagement, thereby changing the position of the piston and adjusting the volume of the bottom chamber.

[0027] Inside this chamber, a magnetic floating plate 6 is vertically mounted. The magnetic floating plate 6 can slide vertically with the liquid level. Its bottom is conical. When there is no liquid in the chamber, the conical bottom contacts the bottom of the chamber of the glass tube 12. In addition, magnetic structures are provided on both sides of the magnetic floating plate 6. The magnetic structures can be either permanent magnet blocks or magnetic coatings.

[0028] Multiple flip plates 14 are rotatably mounted in a circumferential manner at the bottom end of the glass tube 12. Under normal working conditions, the flip plates 14 are in a vertical position and abut against the bottom insertion interface 121 to form a stable mechanical support structure. In addition, a torsion spring is installed at the rotatable connection between the flip plates 14 and the glass tube 12. The torsion spring realizes the flip plate 14 flipping and resetting operation and makes the flip plate 14 always generate a force to rotate in a vertical position. The glass tube 12 and the insertion port 121 are connected by a corrugated pipe 15. The expansion and contraction characteristics of the corrugated pipe 15 allow the insertion port 121 to make a certain vertical displacement. When the flip plate 14 deflects and releases the limit, the insertion port 121 can be pushed upward by external force. The flip plate 14 and the magnetic structure on the outside of the magnetic floating plate 6 attract each other. When the magnetic floating plate 6 drops to the position of the flip plate 14 with the liquid level, the flip plate 14 is attracted by magnetic force and deflects towards the magnetic floating plate 6 and abuts against the conical structure at the bottom of the floating plate. At this time, the flip plate 14 releases the limit on the structure of the insertion port 121 below.

[0029] Furthermore, a movable plate 7 is vertically slidably installed on the outer wall of the insertion interface 121. A vertically extending rod 71 is connected to one side of the movable plate 7. The rod 71 extends upward into the interior of the flexible detection tube, and a seal is provided at the connection between the rod 71 and the flexible detection tube 3. The rod 71 extends upward to the counting disk 10, which is installed on the upper side of the flexible detection tube 3. Part of its wheel surface is exposed from the notch opened on the outside of the flexible detection tube 3 for personnel to read. Multiple numbers are distributed circumferentially on the wheel surface of the counting disk 10. Two layers of stepping staggered toothed disks 22 are coaxially installed at its bottom. The stepping staggered toothed disks 22 are rotatably installed inside the flexible detection tube 3 via a shaft, and a coil spring 23 is provided at the rotatable connection to provide continuous reset torque. The two layers of stepping staggered toothed disks 22 adopt an interlaced tooth design, and wedge-shaped tooth blocks are distributed on their outer edges.

[0030] In its natural state, the top of the vertical rod 71 extends vertically and horizontally, and abuts against the tooth block area of ​​the lower stepping staggered toothed disk 22. When the vertical rod 71 moves vertically upward, the top of the vertical rod 71 will act on the two layers of staggered tooth blocks in sequence, restricting the stepping staggered toothed disk 22 to rotate step by step at a predetermined angle, thereby driving the counting disk 10 to rotate and display the corresponding digital scale.

[0031] Reference Figure 9-12 Inside the flexible detection tube 3, a feeding ball 11 is installed via an auxiliary plate 111. The feeding ball 11 can slide vertically along the inner wall of the flexible detection tube 3. The auxiliary plate 111 has a cone-shaped structure with the tip pointing downwards to effectively balance the extrusion force of the eccentric wheel 4.

[0032] Inside the feeding ball 11, on the bottom side, there is a feeding chamber 112 for containing wolfberry liquid. Multiple micro-holes are opened on its outer wall to allow liquid to seep out. A float rod 16 is vertically arranged in the middle of the feeding chamber 112. A float is connected to the bottom end of the float rod 16, and the rod body is inserted into the inside of the feeding ball 11 and a magnetic trigger plate 161 is provided at the top. The magnetic trigger plate 161 can be vertically slidably arranged in the flow port 20. The bottom end of the flow port 20 is provided with a notch communicating with the feeding chamber 112, and the top end is provided with an outlet. A plug 17 is covered at the outlet. A rubber plug 18 is installed in the middle of the flow port 20 to block the inlet of the plug 17.

[0033] The flow port 20 is vertically fitted with a connector 17 on its outer periphery. The connector 17 is embedded inside the top of the feeding ball 11 in its natural state. Its outer surface has a conical structure to fit the insertion interface 121 at the bottom of the glass tube 12. The insertion interface 121 and the connector 17 are a mutual insertion and docking mechanism. The outer wall of the connector 17 is provided with a stepped surface and is elastically connected to the inside of the feeding ball 11 through a spring 19, so that the spring 19 deforms synchronously when it is in a vertical position.

[0034] When the feeding chamber 112 is filled with liquid, the float rod 16 is driven by buoyancy to move the magnetic trigger plate 161 to float up until it is stopped by the upper wall of the flow port 20. At this time, the magnetic mechanism of the magnetic trigger plate 161 is in a high position. Next, a ball groove is provided on the side wall of the flow port 20. A spring-loaded locking ball 21 is installed in the ball groove and is pre-tightened by a spring. The spring-loaded locking ball 21 is made of magnetic material and is magnetically engaged with the magnetic trigger plate 161. The spring-loaded locking ball 21 is constrained in the ball groove and exposes half of the ball. In its natural state, the spring-loaded locking ball 21 is pushed by the spring and inserted into the slot 171 on the inner wall of the connector 17. The connector 17 is locked in the retracted position by the concave-convex engagement.

[0035] When the liquid level drops due to a decrease in the feeding chamber 112, the float rod 16 moves downward and drives the magnetic trigger plate 161 to descend synchronously. When the magnetic trigger plate 161 descends to a horizontal height that is coplanar with the spring-loaded locking ball 21, the magnetic attraction mechanism on its outer periphery generates a magnetic attraction force, driving the spring-loaded locking ball 21 to move radially inward and disengage from the slot 171. At this time, the insertion nozzle 17 is released from axial locking and pops up and extends out of the feeding ball 11 under the restoring force of the spring 19, thus changing to a trigger state that can be connected with the insertion interface 121.

[0036] In this embodiment, when the device is performing a test, the supply mechanism inside the body 1 introduces a preset dose of probiotic liquid into the reaction chamber of multiple flexible detection tubes 3 through a water pipe. Subsequently, the lifting mechanism inside the body 1 drives the feeding mechanism 2 to descend, so that its output end is connected to the connecting nozzle 9 at the upper end of the flexible detection tube 3.

[0037] During this process, the feeding mechanism 2 initially fills the sealed chamber inside the glass tube 12 with browning wolfberry liquid. At the same time, before the feeding mechanism 2 completes the docking, the personnel can rotate the lifting adjustment plate 131 to drive the piston rod 13 to move vertically to change the bottom volume, so as to adjust the initial volume of the bottom of the glass tube 12 according to the experimental requirements and realize the quantitative control of multi-gradient parallel experiments.

[0038] After the experiment begins, the telescopic rod 5 drives the support frame 51 to move the two sets of eccentric wheels 4 vertically back and forth between the flexible detection tubes 3. Due to the meshing of the toothed grooves on the side wall of the eccentric wheel 4 and the toothed support rod 31, the eccentric wheel 4 generates controlled spin synchronously during the displacement process. The geometric characteristics of the eccentric wheel 4 are used to perform periodic asymmetric compression on the flexible detection tube 3. This compression action not only simulates the physiological peristalsis of the intestine and generates complex fluid shear force to activate the active substances of browning wolfberry, but also drives the feeding ball 11 inside the reaction chamber to achieve synchronous vertical sliding motion along the inner wall of the tube.

[0039] As the feeding ball 11 moves up and down with peristalsis, the wolfberry liquid contained in its internal feeding chamber 112 is evenly dispersed into the probiotic liquid through the micropores on the outer wall in a single quantitative and micropore seepage structure. This effectively avoids the biofeedback inhibition caused by excessively high local concentrations in traditional devices, allowing wolfberry active substances (such as melanoidins, polysaccharides, etc.) to produce rapid and sufficient biochemical reactions with the probiotic population.

[0040] At this time, the laser turbidity sensor 8 at the bottom monitors the changes in absorbance of the bacterial community in real time. The dynamic growth curve is used to quantitatively evaluate the effect of browning wolfberry on the proliferation of the bacterial community. The laser turbidity sensor 8 is a conventional accessory of the active substance detection device and is a conventional technical means. Its specific structure and principle will not be explained further.

[0041] Then, when the liquid level in the feeding chamber 112 inside the feeding ball 11 drops due to continuous reaction, the float rod 16 inside moves down, driving the magnetic trigger plate 161 to move to the rebound locking ball 21. Attracted by the magnetic force, the rebound locking ball 21 radially displaces and disengages from the slot 171, releasing the lock on the plug nozzle 17. The plug nozzle 17 pops up under the action of the spring 19 and enters the docking state. As the feeding ball 11 moves to the top of the stroke again under the drive of the peristaltic mechanism, the popped plug nozzle 17 automatically completes physical docking with the plug interface 121 at the bottom of the glass tube 12, and the wolfberry liquid in the glass tube 12 is replenished into the feeding chamber 112. If there is enough medicine in the feeding chamber 112, the float rod 16 moves upward, and the insertion nozzle 17 is driven upward by the eccentric wheel 4 and connects with the glass tube 12. The resulting clamping force will drive it to retract again. Once there is enough medicine in the feeding chamber 112, the float rod 16 moves upward and locks the insertion nozzle 17, which was originally retracted, again through the spring-loaded locking ball 21. Under normal operating conditions, the connector 17 remains in the retracted state (with sufficient liquid level) and will not engage with the connector 121 for replenishment when it moves up and down with the peristalsis, thus achieving on-demand replenishment.

[0042] As the reaction continues, when the goji berry liquid in the glass tube 12 is depleted, the magnetic floating plate 6 descends to the bottom with the liquid level. Its side wall magnetic structure attracts the flip plate 14 to deflect inward, releasing the vertical restriction on the insertion port 121. At this time, the insertion port 121 gains vertical freedom of movement under the support of the corrugated pipe 15. When the feeding ball 11 below rises again to support the insertion port 121, the insertion port 121 drives the vertical rod 71 to move upward momentarily through the movable plate 7. The top of the vertical rod 71 acts sequentially on the toothed structure on the outside of the two-layer stepping staggered toothed disc 22. Since the stepping staggered toothed disc 22 achieves continuous rotational driving force through the coil spring 23, the counting disc 10 will rotate one scale step to complete the automatic counting of one feeding cycle. By recording the value of the counting disc 10 and the feeding frequency, the operator can intuitively quantify the consumption rate and bioavailability of specific browned goji berry active substances by probiotics.

[0043] In addition, throughout the reaction process, the inlet trachea continuously injects anaerobic mixed gas supplied by the body's supply mechanism 1, while the outlet trachea simultaneously expels waste gases such as carbon dioxide produced by metabolism, maintaining a highly biomimetic anaerobic microenvironment. Compared with traditional static mixed detection, this physical peristalsis and quantitative reaction mechanism significantly improves the sensitivity and physiological simulation of detection, and can accurately reveal the reaction state of active substances in browned wolfberry in a dynamic intestinal environment.

[0044] In addition, it should be noted that the supply mechanism inside the body 1, including components such as the gas source pump, liquid phase metering pump and pressure regulating valve, is a standard configuration in microbial culture devices. Its specific pipeline layout and automatic control program are common knowledge to those skilled in the art and will not be explained further.

[0045] In addition, it should be noted that "body 1" refers to the active substance detection body. The aforementioned insertion and docking mechanism between the insertion nozzle 17 and the insertion interface 121, as well as the insertion and docking end between the feeding mechanism 2 and the connecting nozzle 9, are all standard configurations in laboratories and in this field. In addition, in this embodiment, all fluid transmission, air circulation and dynamic sealing junctions, such as the penetration of the vertical rod 71 and the sliding connection between the plug nozzle 17 and the feeding ball 11, are provided with seals. These seals include, but are not limited to, O-rings, lip rings, mechanical seals or flexible corrugated sleeves, which are all conventional configurations and necessary sealing means in the art, and therefore will not be explained further.

[0046] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A device for detecting active substances in browned wolfberry, comprising a body (1), characterized in that, The body (1) is provided with multiple flexible detection tubes (3) in the middle. Each flexible detection tube (3) has an eccentric wheel (4) that can roll vertically pressed against its outer wall. The eccentric wheel (4) rolls to drive the feeding ball (11) installed in the inner cavity of the flexible detection tube (3) to move vertically. The feeding ball (11) and the glass tube (12) for inputting wolfberry liquid are provided inside the flexible detection tube (3) to form a docking liquid replenishment mechanism. The feeding chamber (112) inside the feeding ball (11) is provided with a float rod (16). The top of the float rod (16) is provided with a magnetic trigger plate (161) and extends into the flow port (20) at the top of the feeding ball (11) for inputting liquid. The float rod (16) rises and falls synchronously with the liquid level inside the feeding chamber (112) and drives the magnetic trigger plate (161) to lock and release with the plug (17) that is movably sleeved on the outer wall of the flow port (20). The glass tube (12) is fed by the feeding mechanism (2). The bottom of the glass tube (12) is provided with an interface (121) that is a mutual insertion mechanism with the insertion nozzle (17). The glass tube (12) and the insertion nozzle (17) are supported and connected by a corrugated pipe (15) and multiple flip plates (14). When the glass tube (12) is at a low liquid level, the magnetic floating plate (6) set inside the tube will descend and attract the flip plate (14) to tilt inward to release the insertion nozzle (17) from moving. When the insertion nozzle (17) is connected with the feeding ball (11), the counting mechanism is activated to count.

2. The device for detecting active substances in browned wolfberry according to claim 1, characterized in that, Toothed support rods (31) are provided on both sides of the flexible detection tube (3) to provide support. A feeding mechanism (2) for introducing browning wolfberry liquid is provided above the flexible detection tube (3). The feeding mechanism (2) is connected to the lifting mechanism inside the machine body (1) to realize the lifting operation. A laser turbidity sensor (8) is installed at the bottom of the flexible detection tube (3). The laser turbidity sensor (8) is electrically connected to the machine body (1).

3. The device for detecting active substances in browned wolfberry according to claim 1, characterized in that, A telescopic rod (5) is provided between the flexible detection tubes (3). A support frame (51) is provided at the top of the telescopic rod (5). Multiple pairs of eccentric wheels (4) are rotatably installed on the outer side of the support frame (51). The eccentric wheels (4) are pressed against the outer wall of the flexible detection tube (3). The side wall of the eccentric wheel (4) is provided with a toothed groove and meshes with the toothed support rod (31).

4. The device for detecting active substances in browned wolfberry according to claim 1, characterized in that, The upper part of the flexible detection tube (3) is equipped with a glass tube (12). A connecting nozzle (9) is detachably installed on the upper part of the glass tube (12). The connecting nozzle (9) is used to connect to the output end of the feeding mechanism (2) to realize the feeding connection. A piston rod (13) is vertically arranged in the center of the glass tube (12). A piston is installed at the bottom of the piston rod (13). The piston and the bottom chamber of the glass tube (12) together form a sealed chamber for containing wolfberry liquid. A laser level gauge (132) is installed on one side of the middle part of the piston. A lifting adjustment plate (131) is rotatably installed on the upper part of the piston rod (13). The outer wall of the lifting adjustment plate (131) is screwed to the internal thread of the upper middle part of the flexible detection tube (3).

5. The device for detecting active substances in browned wolfberry according to claim 1, characterized in that, The bottom of the glass tube (12) is flexibly connected to the insertion interface (121) through a corrugated pipe (15). A magnetic floating plate (6) is vertically and movably installed inside the glass tube (12). The magnetic floating plate (6) can slide vertically with the liquid level. Magnetic structures are provided on both sides of the magnetic floating plate (6). Multiple flip plates (14) are rotatably installed at the bottom of the glass tube (12). The flip plates (14) and the magnetic floating plate (6) are magnetically attracted to each other. Under natural working conditions, the flip plates (14) are in a vertical position and abut against the bottom insertion interface (121) to form a stable mechanical support structure. A torsion spring is installed at the rotatable connection between the flip plates (14) and the glass tube (12).

6. The device for detecting active substances in browned wolfberry according to claim 1, characterized in that, The counting mechanism includes a movable plate (7) that is movably sleeved on the outer wall of the insertion interface (121). A vertical rod (71) extending vertically upward is connected to one side of the movable plate (7). The vertical rod (71) extends upward to the counting disk (10) inside the flexible detection tube (3). The counting disk (10) is installed on the upper side of the flexible detection tube (3). A multi-layer stepping staggered toothed disk (22) is installed at the bottom of the counting disk (10). Each stepping staggered toothed disk (22) is rotatably installed inside the flexible detection tube (3) through a shaft. A coil spring (23) is provided at the rotatable connection. The two layers of stepping staggered toothed disks (22) adopt a staggered tooth design. Multiple wedge-shaped tooth blocks are distributed on their outer edges.

7. The device for detecting active substances in browned wolfberry according to claim 1, characterized in that, The feeding ball (11) has an auxiliary plate (111) on its outer wall and a feeding chamber (112) for containing wolfberry liquid on its inner bottom side. The outer wall of the feeding ball (11) has multiple micro-holes for liquid to seep out. A float rod (16) is vertically provided in the middle of the feeding chamber (112). A float is connected to the bottom of the float rod (16), and the rod is inserted into the inside of the feeding ball (11) and a magnetic trigger plate (161) is provided at the top. The magnetic trigger plate (161) can be vertically slidably arranged in the flow port (20). A plug-in nozzle (17) is vertically sleeved on the outer wall of the flow port (20). The plug-in nozzle (17) is embedded in the top of the feeding ball (11) in its natural state. The plug-in nozzle (17) and the plug-in interface (121) are plug-in hooks to each other. The outer wall of the plug-in nozzle (17) is elastically connected to the inside of the feeding ball (11) by a spring (19).

8. The device for detecting active substances in browned wolfberry according to claim 1, characterized in that, The side wall of the flow port (20) is provided with a ball groove, and a spring-loaded locking ball (21) is installed in the ball groove. The spring-loaded locking ball (21) and the magnetic trigger plate (161) are magnetically attracted to each other. The spring-loaded locking ball (21) is constrained in the ball groove and exposes half of the ball. In its natural state, the spring-loaded locking ball (21) is pushed by the spring and inserted into the slot (171) on the inner wall of the plug (17) to complete the concave-convex locking.

9. The device for detecting active substances in browned wolfberry according to claim 7, characterized in that, A rubber plug (18) is installed in the middle of the flow port (20). When the insertion nozzle (17) is inserted into the feeding ball (11), the insertion nozzle (17) and the rubber plug (18) are squeezed and contacted to achieve sealing.

10. The detection method of the browning wolfberry active substance detection device according to any one of claims 1-9, characterized in that, Includes the following steps: S1. Inject a preset dose of probiotic liquid into the flexible detection tube and introduce mixed gas to maintain an anaerobic microenvironment. At the same time, replenish the glass tube with wolfberry extract through the feeding mechanism. S2. Start the eccentric wheel to rotate and squeeze the tube body, simulate the physiological peristalsis of the intestine to generate physical power, and simultaneously drive the feeding ball inside the tube to slide up and down along the inner wall of the tube to feed the bacteria. Use a laser turbidity sensor to monitor the changes in the absorbance of the bacterial community in real time. S3. When the liquid level in the feeding ball drops, the magnetic attraction mechanism is triggered to release the spout, which then connects with the spout at the bottom of the glass tube during the upward stroke of the feeding ball to replenish the wolfberry liquid. S4. When the liquid in the glass tube is exhausted, the flip plate is deflected and the limit is released. The counting disk is driven to rotate and count by the vertical rod linkage stepping interlocking tooth disk.

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