Multifunctional pipetting device and pipetting method

Abstract

The invention relates to the technical field of pipetting devices, in particular to a multifunctional pipetting device and a pipetting method. Comprising a pipetting gun nozzle, a small-capacity cylinder barrel, a small-capacity piston rod, a small-capacity pipetting power mechanism, a large-capacity cylinder barrel, a large-capacity piston rod, a large-capacity pipetting power mechanism, a small-capacity suction head, a large-capacity suction head and a filtering suction head, the pipetting gun nozzle comprises a small-capacity sleeve head part matched with the small-capacity suction head and a large-capacity sleeve head part matched with the large-capacity suction head or the filtering suction head; a filter screen is arranged in the filter suction head, and when the small-capacity suction head and the filter suction head are both arranged on the pipetting gun nozzle in a sleeving manner, the bottom of the small-capacity suction head is located above the filter screen; the multifunctional pipetting device can be well suitable for the situation that the multifunctional pipetting device needs to be used in cooperation with two suction heads of different specifications and needs to be used for pipetting of two different measuring ranges, and the two functions of pipetting and filtering are integrated.

Description

Technical Field

[0001] This invention relates to the field of pipetting apparatus technology, and more particularly to a multifunctional pipetting apparatus and pipetting method. Background Technology

[0002] Pipettes are key tools for precise liquid transfer in laboratory fields such as biology, chemistry, medicine, and food testing.

[0003] Existing pipetting devices are all single-tip pipetting devices, including a cylinder, a piston rod slidably connected to the cylinder, and a pipetting power mechanism for driving the piston rod. They can only be used with a single-size pipette tip and can only be used for pipetting in a single volume. They are not suitable for situations that require use with two different sizes of pipette tips or for pipetting in two different volume ranges, which has certain limitations. In addition, when performing pipetting operations that require filtration, the sample is often first drawn from the original container using a conventional pipetting device, then discharged into a filter tube with filtration function, and then the filtered liquid is drawn from the filter tube using a new pipetting tip and transferred to the target container such as a detection plate or reaction tube to complete the filtration pipetting. This process is not only cumbersome and inefficient, but also requires multiple transfers of the sample, increasing the risk of sample contamination and the possibility of sample loss. Summary of the Invention

[0004] The purpose of this invention is to provide a multifunctional pipetting device that addresses the shortcomings of existing technologies. This device enables dual-range composite pipetting, allowing for switching between small-volume and large-volume pipetting according to specific usage needs. It is well-suited for situations requiring the use of two different sizes of pipette tips or for pipetting at two different volumes. It offers greater overall flexibility and practicality, integrating pipetting and filtration into one unit. This not only significantly simplifies the operation steps and improves efficiency but also reduces the number of sample transfers, thereby lowering the risk of sample contamination and the possibility of sample loss. This invention also provides a multifunctional pipetting method, which not only has three pipetting modes to adapt to diverse experimental needs, but also significantly simplifies the operation steps and improves efficiency compared to existing filtration pipetting, and can reduce the number of sample transfers, thereby reducing the risk of sample contamination and the possibility of sample loss.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows: a multifunctional pipetting device, comprising a pipetting nozzle, a small-capacity cylinder connected to the pipetting nozzle, a small-capacity piston rod slidably connected in the small-capacity cylinder, a small-capacity pipetting power mechanism for driving the small-capacity piston rod to slide, a large-capacity cylinder connected to the pipetting nozzle, a large-capacity piston rod slidably connected in the large-capacity cylinder, a large-capacity pipetting power mechanism for driving the large-capacity piston rod to slide, a small-capacity pipette tip, a large-capacity pipette tip, and a filter pipette tip. The pipetting nozzle includes a small-capacity sleeve head for fitting the small-capacity pipette tip and a large-capacity sleeve head for fitting the large-capacity pipette tip or the filter pipette tip. The filter pipette tip is provided with a filter screen. When both the small-capacity pipette tip and the filter pipette tip are fitted onto the pipetting nozzle, the bottom of the small-capacity pipette tip is located above the filter screen.

[0006] A further improvement to the above solution is that a multifunctional pipetting device of the present invention further includes a pipetting integrated base, wherein the small-capacity cylinder, the small-capacity pipetting power mechanism, the large-capacity cylinder, and the large-capacity pipetting power mechanism are respectively disposed on the pipetting integrated base, the output end of the small-capacity pipetting power mechanism is driven and connected to the small-capacity piston rod, and the output end of the large-capacity pipetting power mechanism is driven and connected to the large-capacity piston rod.

[0007] A further improvement to the above solution is that a multifunctional pipetting device of the present invention further includes a transfer mechanism, wherein the integrated pipetting base is connected to the end of the transfer mechanism.

[0008] A further improvement to the above solution is that a multifunctional pipetting device of the present invention further includes a pipette tip holder, wherein the pipette tip holder has multiple small-capacity insertion holes for inserting small-capacity pipette tips and multiple large-capacity insertion holes for inserting large-capacity pipette tips or filter pipette tips.

[0009] A further improvement to the above solution is that a multifunctional pipetting device of the present invention further includes a pipette tip retraction holder, wherein the pipette tip retraction holder is formed with a small-capacity notch for a small-capacity pipette tip to extend into in the left-right direction, a small-capacity baffle portion protruding and formed at the front and rear ends of the small-capacity notch portion in the front-back direction, a large-capacity notch for a large-capacity pipette tip or a filter pipette tip to extend into in the left-right direction, and a large-capacity baffle portion protruding and formed at the front and rear ends of the large-capacity notch portion in the front-back direction.

[0010] A further improvement to the above solution is that a multifunctional pipetting device of the present invention further includes a pipette tip collection box disposed below the small-capacity baffle and the large-capacity baffle.

[0011] A further improvement to the above solution is that the small-capacity sleeve head and the large-capacity sleeve head are coaxially arranged, with the small-capacity sleeve head located at the bottom of the large-capacity sleeve head.

[0012] A further improvement to the above solution is that the multifunctional pipetting device of the present invention also includes an air tube, the small-capacity cylinder and the large-capacity cylinder are arranged side by side in the front-to-back direction, the pipetting nozzle is located at the bottom of the small-capacity cylinder and is directly connected to the small-capacity cylinder, and the large-capacity cylinder is connected to the pipetting nozzle through the air tube.

[0013] A multifunctional pipetting method, based on a multifunctional pipetting device according to any of the above-mentioned solutions, includes a small-volume pipetting mode, a large-volume pipetting mode, and a filtration pipetting mode; The small-volume pipetting mode includes the following steps: The small volume pipette tip is fitted onto the small volume sleeve head of the pipette tip. The small volume pipetting power mechanism drives the small volume piston rod to slide upward to complete the aspiration of the small volume reagent. The small volume pipetting power mechanism drives the small volume piston rod to slide downward to complete the dispensing of the small volume reagent. The large-volume pipetting mode includes the following steps: The large-volume pipette tip is fitted onto the large-volume sleeve head of the pipette tip. The large-volume pipetting power mechanism drives the large-volume piston rod to slide upward and complete the aspiration of the large-volume reagent. The large-volume pipetting power mechanism drives the large-volume piston rod to slide downward and complete the dispensing of the large-volume reagent. The filtration and pipetting mode includes the following steps: First, place the small-volume pipette tip onto the small-volume sleeve head of the pipette tip. Drive the small-volume piston rod upward through the small-volume pipette power mechanism and aspirate the original sample through the small-volume pipette tip. Then, place the filter pipette tip onto the large-volume sleeve head of the pipette tip. Drive the small-volume piston rod downward through the small-volume pipette power mechanism and allow the original sample in the small-volume pipette tip to flow through the filter screen of the filter pipette tip and be discharged.

[0014] The beneficial effects of this invention are as follows: This invention provides a multifunctional pipetting device, comprising a pipetting nozzle, a small-capacity cylinder connected to the pipetting nozzle, a small-capacity piston rod slidably connected in the small-capacity cylinder, a small-capacity pipetting power mechanism for driving the small-capacity piston rod to slide, a large-capacity cylinder connected to the pipetting nozzle, a large-capacity piston rod slidably connected in the large-capacity cylinder, a large-capacity pipetting power mechanism for driving the large-capacity piston rod to slide, a small-capacity pipette tip, a large-capacity pipette tip, and a filter pipette tip. The pipetting nozzle includes a small-capacity sleeve head for adapting to the small-capacity pipette tip and a large-capacity sleeve head for adapting to the large-capacity pipette tip or the filter pipette tip. The filter pipette tip is provided with a filter screen. When both the small-capacity pipette tip and the filter pipette tip are fitted onto the pipetting nozzle, the bottom of the small-capacity pipette tip is located above the filter screen. The multifunctional pipetting device of this invention provides pipetting tips that can be adapted to both small-volume tips (e.g., 1ml tips) and large-volume tips (e.g., 10ml tips). Through the coordinated use of a small-volume cylinder, a small-volume piston rod, a small-volume pipetting power mechanism, and a large-volume cylinder, a large-volume piston rod, and a large-volume pipetting power mechanism, dual-volume composite pipetting can be achieved. This allows for switching between small-volume and large-volume pipetting according to specific usage needs. It is well-suited for situations requiring compatibility with two different tip sizes and for pipetting at two different volume ranges. The overall flexibility and practicality are significantly enhanced. This multifunctional pipetting device of the present invention, through its coordinated design… This device integrates pipetting and filtration functions into one unit, featuring small-volume pipette tips, filter pipette tips with built-in filters, and pipette nozzles. The small-volume pipette tip is used to aspirate the original sample, while the filter pipette tip has a built-in filter for filtration. During operation, simply place the small-volume pipette tip onto the small-volume head of the pipette nozzle to aspirate the sample, and then place the filter pipette tip onto the large-volume head. The sample is then filtered by draining the liquid through the filter. The entire process eliminates the need to transfer the sample to an additional filter tube, change pipetting devices, or repeatedly insert and remove different pipette tips. This significantly simplifies the operation, improves efficiency, and reduces the number of sample transfers, thereby reducing the risk of sample contamination and potential sample loss. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the structure of the present invention.

[0016] Figure 2 This is a schematic diagram of the pipette tip, small-capacity cylinder, small-capacity piston rod, large-capacity cylinder, and large-capacity piston rod of the present invention.

[0017] Figure 3 for Figure 1 Enlarged view of point A in the middle.

[0018] Explanation of reference numerals in the attached drawings: 1. Pipette assembly base; 2. Pipette tip; 21. Small-capacity sleeve head; 22. Large-capacity sleeve head; 3. Small-capacity cylinder; 4. Small-capacity piston rod; 5. Small-capacity pipetting power mechanism; 6. Large-capacity cylinder; 7. Large-capacity piston rod; 8. Large-capacity pipetting power mechanism; 9. Transfer mechanism; 10. Tip holder; 10. Small-capacity insertion port; 102. Large-capacity insertion port; 11. Tip retraction holder; 111. Small-capacity notch; 112. Small-capacity retaining edge; 113. Large-capacity notch; 114. Large-capacity retaining edge; 12. Tip collection box; 13. Air tube; 14. Small-capacity tip; 15. Large-capacity tip; 16. Filter tip; 17. Filter screen. Detailed Implementation

[0019] Example 1, as Figure 1-3As shown, a multifunctional pipetting device of the present invention includes a pipetting nozzle 2, a small-capacity cylinder 3 connected to the pipetting nozzle 2, a small-capacity piston rod 4 slidably connected in the small-capacity cylinder 3, a small-capacity pipetting power mechanism 5 for driving the small-capacity piston rod 4 to slide, a large-capacity cylinder 6 connected to the pipetting nozzle 2, a large-capacity piston rod 7 slidably connected in the large-capacity cylinder 6, a large-capacity pipetting power mechanism 8 for driving the large-capacity piston rod 7 to slide, a small-capacity pipette tip 14, a large-capacity pipette tip 15, and a filter pipette tip 16. The pipetting nozzle 2 includes components adapted to fit the small-capacity pipette tip 14. The device includes a small-capacity pipette head 21 and a large-capacity pipette head 22 for fitting with a large-capacity pipette tip 15 or a filter pipette tip 16. The filter pipette tip 16 has a filter screen 17 inside. When both the small-capacity pipette tip 14 and the filter pipette tip 16 are fitted onto the pipette nozzle 2, the bottom of the small-capacity pipette tip 14 is positioned above the filter screen 17. The pipette nozzle 2 of this multifunctional pipetting device can be adapted to two different specifications of pipette tips: a small-capacity pipette tip 14 (e.g., a 1ml tip) and a large-capacity pipette tip 15 (e.g., a 10ml tip). This is achieved through a small-capacity cylinder 3, a small-capacity piston rod 4, a small-capacity pipetting power mechanism 5, and a large-capacity cylinder. The combination of the cylinder 6, the large-capacity piston rod 7, and the large-capacity pipetting power mechanism 8 enables dual-range composite pipetting, allowing switching between small-volume and large-volume pipetting according to specific usage needs. It is well-suited for situations requiring use with two different sizes of pipette tips or for pipetting at two different volume ranges, offering greater overall flexibility and practicality. This multifunctional pipetting device integrates pipetting and filtration functions into one unit by setting up a cooperating small-volume pipette tip 14, a filter pipette tip 16 with a filter screen 17, and a pipetting nozzle 2. The small-volume pipette tip 14 is used for aspiration... Take the original sample, and use the built-in filter screen 17 of the filter tip 16 for filtration. During operation, simply put the small volume tip 14 on the small volume sleeve head 21 of the pipette tip 2 to complete the sample aspiration, and then put the filter tip 16 on the large volume sleeve head 22. The sample can be filtered by draining the liquid and letting it flow through the filter screen 17. The whole process does not require transferring the sample to an additional filter tube, nor does it require changing the pipette or repeatedly inserting and removing different tips. This not only significantly simplifies the operation steps and improves efficiency, but also reduces the number of sample transfers, thereby reducing the risk of sample contamination and the possibility of sample loss.

[0020] The multifunctional pipetting device of the present invention further includes a pipetting integrated base 1. The small-capacity cylinder 3, the small-capacity pipetting power mechanism 5, the large-capacity cylinder 6, and the large-capacity pipetting power mechanism 8 are respectively disposed on the pipetting integrated base 1. The output end of the small-capacity pipetting power mechanism 5 is driven and connected to the small-capacity piston rod 4, and the output end of the large-capacity pipetting power mechanism 8 is driven and connected to the large-capacity piston rod 7.

[0021] The multifunctional pipetting device of the present invention further includes a transfer mechanism 9. The pipetting integrated base 1 is connected to the end of the transfer mechanism 9. The transfer mechanism 9 is a mechanism with a running track in any one, two, or three directions of the X-axis, Y-axis, and Z-axis. The transfer mechanism 9 is a well-known technology and will not be described in detail here. The transfer mechanism 9 can drive the entire multifunctional pipetting device of the present invention to move and realize active pipetting. Of course, in other embodiments, the transfer mechanism 9 may not be required. The multifunctional pipetting device of the present invention is relatively fixed and passive pipetting is realized by driving the sample of the liquid to be aspirated to move.

[0022] The multifunctional pipetting device of the present invention also includes a pipette tip holder 10, which has multiple small-capacity insertion holes 101 for inserting small-capacity pipette tips 14 and multiple large-capacity insertion holes 102 for inserting large-capacity pipette tips 15 or filter pipette tips 16. The pipette tip holder 10 can better realize the centralized and orderly storage of various small-capacity pipette tips 14, large-capacity pipette tips 15 and filter pipette tips 16, thereby making it easier to retrieve the small-capacity pipette tips 14, large-capacity pipette tips 15 and filter pipette tips 16 in the future.

[0023] A multifunctional pipetting device of the present invention further includes a pipette tip holder 11, wherein the pipette tip holder 11 is formed with a small-capacity notch 111 for a small-capacity pipette tip 14 to be inserted in the left-right direction, a small-capacity baffle 112 protruding from the front and rear ends of the small-capacity notch 111 in the front-back direction, a large-capacity notch 113 for a large-capacity pipette tip 15 or a filter pipette tip 16 to be inserted in the left-right direction, and a large-capacity baffle 114 protruding from the front and rear ends of the large-capacity notch 113 in the front-back direction; when a pipette tip 2 fitted with a small-capacity pipette tip 14 is inserted into the small-capacity notch 111 in the left-right direction and then moved upward, the small-capacity baffle 112... The small-volume pipette tip 14, fitted onto the pipette tip 2, is pressed downwards. As the pipette tip 2 moves upwards, the small-volume pipette tip 14 is pulled off the pipette tip 2, thus completing the removal of the small-volume pipette tip 14. Similarly, the pipette tip 2, fitted with a large-volume pipette tip 15 or a filter pipette tip 16, is inserted into the large-volume notch 113 in the left-right direction and then moved upwards. The large-volume baffle 114 presses downwards against the large-volume pipette tip 15 or the filter pipette tip 16 fitted onto the pipette tip 2. As the pipette tip 2 moves upwards, the large-volume pipette tip 15 or the filter pipette tip 16 is pulled off the pipette tip 2, thus completing the removal of the large-volume pipette tip 15 or the filter pipette tip 16.

[0024] The multifunctional pipetting device of the present invention also includes a pipette tip collection box 12 disposed below the small-capacity baffle portion 112 and the large-capacity baffle portion 114. The pipette tip collection box 12 can collect the small-capacity pipette tip 14, the large-capacity pipette tip 15, and the filter pipette tip 16 that are removed, thereby preventing them from being scattered everywhere and keeping the workbench clean.

[0025] The small-capacity nozzle head 21 and the large-capacity nozzle head 22 are coaxially arranged, with the small-capacity nozzle head 21 located at the bottom of the large-capacity nozzle head 22. This not only ensures that the installation and unloading directions of various nozzles are consistent, but also makes the overall structure more compact and reasonable.

[0026] The multifunctional pipetting device of the present invention also includes an air tube 13. The small-capacity cylinder 3 and the large-capacity cylinder 6 are arranged side by side in the front-to-back direction. The pipetting nozzle 2 is located at the bottom of the small-capacity cylinder 3 and is directly connected to the small-capacity cylinder 3. The large-capacity cylinder 6 is connected to the pipetting nozzle 2 through the air tube 13. The overall structure layout is more compact and reasonable.

[0027] Example 2: A multifunctional pipetting method of the present invention, based on a multifunctional pipetting device described in Example 1, includes a small-volume pipetting mode, a large-volume pipetting mode, and a filtration pipetting mode; The small-volume pipetting mode includes the following steps: The small volume pipette tip 14 is fitted onto the small volume sleeve head 21 of the pipette tip 2. The small volume piston rod 4 is driven to slide upward by the small volume pipetting power mechanism 5 to complete the aspiration of the small volume reagent. The small volume piston rod 4 is driven to slide downward by the small volume pipetting power mechanism 5 to complete the discharge of the small volume reagent. The large-volume pipetting mode includes the following steps: The large-volume pipette tip 15 is fitted onto the large-volume sleeve head 22 of the pipette tip 2. The large-volume piston rod 7 is driven to slide upward by the large-volume pipetting power mechanism 8 to complete the aspiration of the large-volume reagent. The large-volume piston rod 7 is driven to slide downward by the large-volume pipetting power mechanism 8 to complete the discharge of the large-volume reagent. The filtration and pipetting mode includes the following steps: First, the small-volume pipette tip 14 is fitted onto the small-volume sleeve head 21 of the pipette tip 2. The small-volume piston rod 4 is driven upward by the small-volume pipetting power mechanism 5 and the original sample is aspirated through the small-volume pipette tip 14. Then, the filter pipette tip 16 is fitted onto the large-volume sleeve head 22 of the pipette tip 2. The small-volume piston rod 4 is driven downward by the small-volume pipetting power mechanism 5 and the original sample in the small-volume pipette tip 14 flows through the filter screen 17 of the filter pipette tip 16 and is discharged. The present invention provides a multifunctional pipetting method that not only has three pipetting modes to adapt to diverse experimental needs, but also significantly simplifies the operation steps and improves efficiency compared to existing filtration pipetting, and reduces the number of sample transfers, thereby reducing the risk of sample contamination and the possibility of sample loss.

[0028] Working principle: Small-volume reagent pipetting: The pipette tip 2 is moved to the position of the small-volume pipette head 14 by the transfer mechanism 9 and the small-volume pipette head 14 is inserted. The small-volume pipette head 14 is fitted onto the small-volume head 21 of the pipette tip 2, completing the installation of the small-volume pipette head 14; The pipette tip 2 with the small-volume pipette head 14 is moved to the position where the small-volume reagent is to be aspirated by the transfer mechanism 9; The small-volume piston rod 4 is driven to slide upward by the small-volume pipetting power mechanism 5 to aspirate the small-volume reagent; The pipette tip 2 with the aspirated small-volume reagent is moved to the position where it is to be added by the transfer mechanism 9; The small-volume pipetting power mechanism 5 is then used to slide the small-volume piston rod 4 upward and aspirate the small-volume reagent; Finally, the pipette tip 2 with the aspirated small-volume reagent is moved to the position where it is to be added by the small-volume pipetting power mechanism 5. The force mechanism 5 drives the small-volume piston rod 4 to slide downward and discharge the small-volume reagent, completing the pipetting of the small-volume reagent. The transfer mechanism 9 drives the pipette nozzle 2, which is equipped with the small-volume tip 14, to move to the tip holder 11 to complete the removal of the small-volume tip 14. Specifically, the pipette nozzle 2, which is fitted with the small-volume tip 14, is inserted into the small-volume notch 111 in the left and right direction and then moved upward. The small-volume baffle 112 presses down on the small-volume tip 14 fitted on the pipette nozzle 2. As the pipette nozzle 2 moves upward, the small-volume tip 14 is pulled off the pipette nozzle 2, thereby completing the removal of the small-volume tip 14. Large-volume reagent pipetting: The pipette tip 2 is moved to the position of the large-volume pipette head 15 by the transfer mechanism 9 and the large-volume pipette head 15 is inserted. The large-volume pipette head 15 is fitted onto the large-volume head 22 of the pipette tip 2, completing the installation of the large-volume pipette head 15. The pipette tip 2 with the large-volume pipette head 15 is moved to the position where the large-volume reagent is to be aspirated by the transfer mechanism 9. The large-volume pipetting power mechanism 8 drives the large-volume piston rod 7 to slide upward and aspirate the large-volume reagent. The pipette tip 2 with the aspirated large-volume reagent is moved to the position where it is to be added by the transfer mechanism 9. The force mechanism 8 drives the large-capacity piston rod 7 to slide downward and discharge the large-capacity reagent, completing the pipetting of the large-capacity reagent. The transfer mechanism 9 drives the pipette tip 2, which is equipped with the large-capacity pipette tip 15, to move to the pipette tip holder 11 to complete the removal of the large-capacity pipette tip 15. Specifically, the pipette tip 2, which is fitted with the large-capacity pipette tip 15, is inserted into the large-capacity notch 113 in the left and right direction and then moved upward. The large-capacity baffle 114 presses the large-capacity pipette tip 15 fitted on the pipette tip 2 downward. As the pipette tip 2 moves upward, the large-capacity pipette tip 15 is pulled off the pipette tip 2, thereby completing the removal of the large-capacity pipette tip 15. Filtration and pipetting: The pipette tip 2 is moved to the position of the small-volume pipette tip 14 by the transfer mechanism 9 and the small-volume pipette tip 14 is inserted. The small-volume pipette tip 14 is fitted onto the small-volume sleeve head 21 of the pipette tip 2, completing the installation of the small-volume pipette tip 14; The pipette tip 2 with the small-volume pipette tip 14 installed is moved to the position of the original sample to be aspirated by the transfer mechanism 9, and the small-volume piston rod 4 is driven upward by the small-volume pipetting power mechanism 5 to aspirate the original sample, completing the aspiration of the original sample; The pipette tip 2 is moved to the position of the filter pipette tip 16 by the transfer mechanism 9 and the filter pipette tip 16 is inserted. The filter pipette tip 16 is fitted onto the large-volume sleeve head 22 of the pipette tip 2, completing the installation of the filter pipette tip 16; The transfer mechanism 9 is driven... The pipette tip 2 moves above the target container, and the small-volume piston rod 4 is driven downward by the small-volume pipetting power mechanism 5 to discharge the original sample. The original sample in the small-volume pipette tip 14 flows through the filter screen 17 in the filter pipette tip 16 and is transferred to the target container, completing the filtration and pipetting. The pipette tip 2 with the filter pipette tip 16 is driven to move to the pipette tip holder 11 by the transfer mechanism 9 to complete the removal of the filter pipette tip 16. Specifically, the pipette tip 2 with the filter pipette tip 16 is inserted into the large-volume notch 113 in the left and right direction and then moved upward. The large-volume baffle 114 presses the filter pipette tip 16 on the pipette tip 2 downward. As the pipette tip 2 moves upward, the filter pipette tip 16 is pulled off the pipette tip 2, thus completing the removal of the filter pipette tip 16.

[0029] Of course, the above description is only a preferred embodiment of the present invention. Therefore, all equivalent changes or modifications made to the structure, features and principles described in the claims of this patent application are included in the scope of this patent application.

Claims

1. A multifunctional pipetting device, characterized in that: It includes a pipette tip (2), a small-capacity cylinder (3) connected to the pipette tip (2), a small-capacity piston rod (4) slidably connected in the small-capacity cylinder (3), a small-capacity pipetting power mechanism (5) for driving the small-capacity piston rod (4) to slide, a large-capacity cylinder (6) connected to the pipette tip (2), a large-capacity piston rod (7) slidably connected in the large-capacity cylinder (6), a large-capacity pipetting power mechanism (8) for driving the large-capacity piston rod (7) to slide, and a small-capacity pipette tip (14). Large-volume pipette tip (15) and filter pipette tip (16). The pipette tip (2) includes a small-volume sleeve head (21) for matching with a small-volume pipette tip (14) and a large-volume sleeve head (22) for matching with a large-volume pipette tip (15) or a filter pipette tip (16). The filter pipette tip (16) is provided with a filter screen (17). When both the small-volume pipette tip (14) and the filter pipette tip (16) are fitted on the pipette tip (2), the bottom of the small-volume pipette tip (14) is located above the filter screen (17).

2. The multifunctional pipetting device according to claim 1, characterized in that: It also includes a pipetting assembly (1), wherein the small-capacity cylinder (3), the small-capacity pipetting power mechanism (5), the large-capacity cylinder (6), and the large-capacity pipetting power mechanism (8) are respectively disposed on the pipetting assembly (1), the output end of the small-capacity pipetting power mechanism (5) is driven to connect to the small-capacity piston rod (4), and the output end of the large-capacity pipetting power mechanism (8) is driven to connect to the large-capacity piston rod (7).

3. A multifunctional pipetting device according to claim 2, characterized in that: It also includes a transfer mechanism (9), wherein the pipetting assembly (1) is connected to the end of the transfer mechanism (9).

4. The multifunctional pipetting device according to claim 1, characterized in that: It also includes a suction head holder (10), which has multiple small-capacity insertion holes (101) for inserting small-capacity suction heads (14) and multiple large-capacity insertion holes (102) for inserting large-capacity suction heads (15) or filter suction heads (16).

5. A multifunctional pipetting device according to claim 1, characterized in that: It also includes a suction head retraction frame (11), which is formed with a small capacity notch (111) for a small capacity suction head (14) to extend into in the left and right direction, a small capacity baffle (112) protruding in the front and rear ends of the small capacity notch (111) respectively, a large capacity notch (113) for a large capacity suction head (15) or a filter suction head (16) to extend into in the left and right direction, and a large capacity baffle (114) protruding in the front and rear ends of the large capacity notch (113) respectively.

6. A multifunctional pipetting device according to claim 5, characterized in that: It also includes a suction head collection box (12) located below the small-capacity baffle (112) and the large-capacity baffle (114).

7. A multifunctional pipetting device according to claim 1, characterized in that: The small capacity sleeve head (21) and the large capacity sleeve head (22) are coaxially arranged, and the small capacity sleeve head (21) is located at the bottom of the large capacity sleeve head (22).

8. A multifunctional pipetting device according to claim 1, characterized in that: It also includes an air tube (13), the small capacity cylinder (3) and the large capacity cylinder (6) are arranged side by side in the front-to-back direction, the pipette nozzle (2) is located at the bottom of the small capacity cylinder (3) and is directly connected to the small capacity cylinder (3), and the large capacity cylinder (6) is connected to the pipette nozzle (2) through the air tube (13).

9. A multifunctional pipetting method, characterized in that: A multifunctional pipetting device according to any one of claims 1-8 includes a small-volume pipetting mode, a large-volume pipetting mode, and a filtration pipetting mode; The small-volume pipetting mode includes the following steps: The small volume pipette tip (14) is fitted onto the small volume sleeve head (21) of the pipette tip (2). The small volume piston rod (4) is driven to slide upward by the small volume pipetting power mechanism (5) to complete the aspiration of the small volume reagent. The small volume piston rod (4) is driven to slide downward by the small volume pipetting power mechanism (5) to complete the discharge of the small volume reagent. The large-volume pipetting mode includes the following steps: The large-volume pipette tip (15) is fitted onto the large-volume sleeve head (22) of the pipette tip (2). The large-volume piston rod (7) is driven upward by the large-volume pipetting power mechanism (8) to complete the aspiration of the large-volume reagent. The large-volume piston rod (7) is driven downward by the large-volume pipetting power mechanism (8) to complete the discharge of the large-volume reagent. The filtration and pipetting mode includes the following steps: First, place the small volume pipette tip (14) onto the small volume sleeve head (21) of the pipette tip (2). Drive the small volume piston rod (4) upward through the small volume pipetting power mechanism (5) and aspirate the original sample through the small volume pipette tip (14). Then, place the filter pipette tip (16) onto the large volume sleeve head (22) of the pipette tip (2). Drive the small volume piston rod (4) downward through the small volume pipetting power mechanism (5) and discharge the original sample in the small volume pipette tip (14) after it flows through the filter screen (17) of the filter pipette tip (16).

Images