Calibration system and calibration method for fluorescent temperature measurement demodulator

Abstract

The invention relates to the technical field of fluorescence temperature measurement, and discloses a calibration system and a calibration method for a fluorescence temperature measurement demodulator, which are used for generating an optical signal for simulating a fluorescence signal in a manner that the intensity of the emitted optical signal changes according to a specified function so as to replace an optical signal returned by a sensor. Therefore, a photoelectric diode of the demodulator receives a photoelectric signal of which the intensity changes according to a function and converts the photoelectric signal into an electric signal, and after the demodulator analyzes the electric signal, the demodulator corrects a direct error between a restored parameter and a standard parameter sent by the calibration device and takes the direct error as a deviation correction value. Therefore, the channel error of the demodulator is corrected by using the standard optical signal so as to calibrate the demodulator.

Description

Technical Field

[0001] This invention relates to the field of fluorescence thermometry, and more specifically to a calibration system and calibration method for a fluorescence thermometer demodulator. Background Technology

[0002] In existing fluorescence thermometry solutions, the calibration process for the fluorescence thermometer and demodulator involves connecting the demodulator and sensor, then placing the sensor in a constant-temperature oil bath, such as... Figure 1 As shown, the oil tank is set to the specified temperature, and then the host computer sends a command to the demodulator to notify the demodulator of the current oil tank temperature, calibrate the corresponding fluorescence lifetime value, and complete the calibration and matching of the sensor and demodulator.

[0003] In reality, the consistency of each batch of sensors is good, but the error between the channels of the demodulator is large. In fact, the calibration and matching process is mainly to complete the error calibration of the demodulator.

[0004] When mass-producing, each sensor and demodulator must be calibrated in this way. This results in significant production efficiency and costs. In addition, there is a substantial investment in temperature-controlled oil baths, and calibration requires a long time for heating and cooling, or multiple oil baths with different temperature points, increasing the workload of moving sensors between different oil baths. Summary of the Invention

[0005] In view of the deficiencies and shortcomings of existing technologies, the present invention aims to provide a calibration system and method for a fluorescence thermometer demodulator, employing a separate calibration approach for the demodulator and sensor. For demodulator calibration, a simulated fluorescence signal is generated by varying the intensity of the emitted light signal according to a specified function. This simulated fluorescence signal is then used to replace the light signal returned by the sensor. The demodulator's photodiode receives this photoelectric signal with varying intensity and converts it into an electrical signal. After analyzing the electrical signal, the demodulator corrects the error between the reconstructed parameters and the standard parameters emitted by the calibration device, using this correction value as a deviation correction value. This achieves demodulator calibration by correcting channel errors using a standard light signal. For sensor calibration, a single fixed constant-temperature oil bath is used to test the consistency within the calibrated demodulator, thus achieving paired calibration of the sensor and demodulator and temperature calibration.

[0006] According to a first aspect of the present invention, a calibration system for a fluorescence temperature demodulator is provided, comprising a fluorescence temperature demodulator to be calibrated, a calibration device, and an optical fiber guide beam. The fiber optic beam has a merging end and a branching end. The merging end is connected to the fiber optic interface of the fluorescence temperature demodulator, and the two branching ends are respectively connected to the optical signal transmitting interface and the optical signal receiving interface of the calibration device. The fluorescence temperature demodulator is configured to send optical pulses at preset time intervals after power-on. The pulses enter the calibration device through its optical fiber interface, the merging end of the optical fiber guide beam, the first branch end of the optical fiber guide beam, and the optical signal receiving interface. The calibration device performs synchronous control accordingly and triggers the emission of simulated fluorescence signals based on the pulse signal. The calibration device is equipped with a processing unit and an analog fluorescence signal transmitting unit connected to the processing unit; the processing unit is configured to generate digital instructions for analog fluorescence signals, and the analog fluorescence signal transmitting unit is configured to convert analog electrical signals into optical signals according to the digital instructions of the processing unit, output analog fluorescence signals with variable light intensity according to a preset function law, and reach the fluorescence temperature demodulator in sequence through the optical signal transmitting interface, the first branch end and the merging end of the optical fiber guide beam. The fluorescence temperature demodulator is configured to analyze the received light signal to obtain the fluorescence lifetime value and calculate the intensity of the light signal, and then return the intensity signal of the light signal to the calibration device through the communication interface. The processing unit of the calibration device determines whether the fluorescence temperature demodulator has stably obtained the optical signal based on the returned optical signal, and controls the generation and transmission of the standard fluorescence lifetime value and calibration command to the fluorescence temperature demodulator in response to the stable signal strength. The fluorescence temperature demodulator stores the difference between the obtained fluorescence lifetime value and the standard fluorescence lifetime value as a calibration value according to the received calibration command, which is used for compensation of the fluorescence temperature demodulator during actual temperature detection.

[0007] According to a second aspect of the present invention, a calibration method for a fluorescence thermometer demodulator is provided, comprising the following steps: After the system is powered on, the fluorescent temperature demodulator sends light pulses at preset time intervals. The light pulses are transmitted into the calibration device through the optical fiber beam guide. The optical signal receiving circuit of the calibration device converts them into square wave trigger signals and transmits them to the processing unit. The processing unit generates an analog electrical signal with a preset function according to the end position of the square wave signal through the DAC interface. After being amplified by the light emission control circuit, the signal drives the light emitter to emit light, converting the analog electrical signal into an analog fluorescent signal, which is then transmitted into the fluorescent temperature demodulator through the fiber optic beam guide. The fluorescence temperature demodulator analyzes the received analog fluorescence signal to obtain the corresponding fluorescence lifetime value and the signal intensity of the received optical signal, and feeds it back to the calibration device through serial communication. The calibration device compares the returned signal strength with the preset adaptation range, and generates a light intensity adjustment command output through the DAC control of the processing unit to adjust the light intensity of the light emitter, forming a closed-loop regulation of repetitive signal generation and intensity feedback until the signal strength is stable; After the signal strength stabilizes, the calibration device sends the standard fluorescence lifetime value and calibration command to the fluorescence temperature demodulator via serial communication. The fluorescence temperature demodulator stores the difference between the obtained fluorescence lifetime value and the standard fluorescence lifetime value as a calibration value based on the received calibration command. This value is used to compensate for the fluorescence temperature demodulator during actual temperature detection.

[0008] As an optional implementation, the preset function law adopts one of the following: sine function, exponential function, triangular wave, and square wave.

[0009] The fluorescence thermometry demodulator system and calibration method described in the above embodiments of the present invention, by designing a calibration device capable of simulating the generation process of fluorescence light signals, allows the light intensity to simulate fluorescence signals according to a set functional law. This enables the calibration of fluorescence fiber optic temperature demodulator channels using simulated fluorescence thermometry signals. The system calibration method for the demodulator and fiber optic sensor in the temperature calibration process can be changed to component-by-component calibration, allowing for error correction in each channel of the demodulator and matching of identical parameters to the sensors, thereby reducing production costs and improving production efficiency. Simultaneously, using standard signals for channel calibration solves the problem of channel consistency and facilitates the separation of sensor errors and channel errors.

[0010] Compared with the prior art, the significant advantages of the fluorescence thermometer demodulator system and calibration method of the present invention are as follows: The calibration device simulates the generation process of fluorescence signals. Since the simulated light signal can be set with fixed parameters, the accuracy and consistency of the light signal can be guaranteed. This avoids the need to maintain the temperature of the sensor and the stability of the generated fluorescence signal in a standard oil bath when calibrating the demodulator using the sensor. The calibration device designed in this invention changes the intensity of the emitted light signal according to a specified function, so that the photodiode of the demodulator receives the photoelectric signal whose intensity changes according to the function and converts it into an electrical signal. The demodulator fits and restores the key parameters of the electrical signal, and records the error between the electrical signal and the standard parameters emitted by the calibration device as a correction for the deviation. This achieves the calibration of the demodulator by correcting the channel error of the demodulator using a standard light signal.

[0011] It should be understood that all combinations of the foregoing concepts and the additional concepts described in more detail below may be considered part of the inventive subject matter of this disclosure, provided that such concepts do not contradict each other. Furthermore, all combinations of the claimed subject matter are considered part of the inventive subject matter of this disclosure.

[0012] The foregoing and other aspects, embodiments, and features of the teachings of the present invention will be more fully understood from the following description in conjunction with the accompanying drawings. Other additional aspects of the invention, such as features and / or beneficial effects of exemplary embodiments, will become apparent from the following description or may be learned through practice of specific embodiments according to the teachings of the present invention. Attached Figure Description

[0013] The accompanying drawings are not intended to be drawn to scale. In the drawings, each identical or nearly identical component shown in the various figures may be denoted by the same reference numeral. For clarity, not every component is labeled in each figure. Embodiments of various aspects of the invention will now be described by way of example and with reference to the accompanying drawings.

[0014] Figure 1 This is a schematic diagram illustrating the calibration principle of the fluorescence temperature sensor and demodulator in a traditional fluorescence temperature measurement scheme.

[0015] Figure 2 This is a schematic diagram of a calibration system for a fluorescence thermometer demodulator according to an embodiment of the present invention.

[0016] Figure 3 This is a schematic diagram of the principle of a fluorescence thermometer demodulator according to an embodiment of the present invention.

[0017] Figure 4 This is a schematic flowchart of a fluorescence thermometer demodulator calibration method according to an embodiment of the present invention. Detailed Implementation

[0018] To better understand the technical content of the present invention, specific embodiments are described below in conjunction with the accompanying drawings.

[0019] Various aspects of the invention are described in this disclosure with reference to the accompanying drawings, which illustrate numerous illustrative embodiments. The embodiments of this disclosure are not necessarily intended to encompass all aspects of the invention. It should be understood that the various concepts and embodiments described above, as well as those described in more detail below, can be implemented in any of many ways, because the concepts and embodiments disclosed herein are not limited to any particular implementation. Furthermore, some aspects of the invention disclosed may be used alone or in any suitable combination with other aspects of the invention disclosed.

[0020] {Example 1} Combination Figure 2 As shown, a calibration system for a fluorescence temperature demodulator according to an exemplary embodiment of the present invention includes a fluorescence temperature demodulator to be calibrated, a calibration device, and an optical fiber guide beam.

[0021] like Figure 2 As shown, an optical transmission path between the fluorescence temperature demodulator and the calibration device is constructed using an optical fiber guide beam.

[0022] As an optional embodiment, the fiber optic beam guide has a merging end and a branching end. The merging end is connected to the fiber optic interface of the fluorescence temperature demodulator, and the two branching ends are respectively connected to the optical signal transmitting interface and the optical signal receiving interface of the calibration device.

[0023] The fluorescence temperature demodulator is configured to send optical pulses at preset time intervals after power-on. The pulses enter the calibration device through its fiber optic interface, the merging end of the fiber optic beam, the first branch end of the fiber optic beam, and the optical signal receiving interface. The calibration device performs synchronous control accordingly and simulates fluorescence signal emission based on the pulse signal trigger.

[0024] The calibration device is equipped with a processing unit and an analog fluorescence signal transmitting unit connected to the processing unit. The processing unit is configured to generate digital instructions for analog fluorescence signals. The analog fluorescence signal transmitting unit is configured to convert analog electrical signals into optical signals according to the digital instructions of the processing unit, output analog fluorescence signals with variable light intensity according to a preset function law, and reach the fluorescence temperature demodulator in sequence through the optical signal transmitting interface, the first branch end of the optical fiber guide beam and the merging end.

[0025] like Figure 3 The diagram illustrates a typical principle of a fluorescence temperature demodulator. The fluorescence temperature demodulator is configured to analyze the received optical signal to obtain a fluorescence lifetime value and calculate the intensity of the optical signal, then return the intensity signal to a calibration device via a communication interface.

[0026] The processing unit of the calibration device determines whether the fluorescence temperature demodulator has stably obtained the optical signal based on the returned optical signal, and controls the generation and transmission of the standard fluorescence lifetime value and calibration command to the fluorescence temperature demodulator in response to the stable signal intensity. The fluorescence temperature demodulator stores the difference between the obtained fluorescence lifetime value and the standard fluorescence lifetime value as a calibration value based on the received calibration command. This value is used to compensate for the fluorescence temperature demodulator during actual temperature detection.

[0027] like Figure 2 In the example shown, the calibration device is equipped with a first collimating lens and a second collimating lens. The first collimating lens is used to collimate the emitted analog fluorescence signal and send it into the optical signal transmitting interface, and the second collimating lens is used to collimate the optical signal returned from the optical signal receiving interface and send it into the photodiode.

[0028] As an optional implementation method, such as Figure 2 As shown, the calibration device has a first serial port module, which is connected to the serial port of the fluorescence temperature demodulator via a communication cable to achieve serial communication.

[0029] like Figure 2 The calibration device has a second serial port module for connecting to a serial port display to achieve status monitoring and visualization. For example, real-time data (signal strength, standard value, calibration status, etc.) can be sent to the serial port display via serial communication, allowing operators to intuitively monitor the calibration progress.

[0030] like Figure 2 As shown, the simulated fluorescence signal transmitting unit includes a light-emitting control circuit and a light emitter. The light emitter is a laser generator or an LED light emitter, such as a blue LED. The processing unit drives the light emitter to emit a simulated fluorescence signal with variable intensity by controlling the light-emitting control circuit. The light intensity is configured to change according to a preset function. Optionally, the preset function can be one of a sine function, an exponential function, a triangular wave, or a square wave.

[0031] Of course, in another embodiment, the aforementioned calibration device can also be configured to generate light emission control with different functions, including but not limited to the aforementioned sine function, exponential function, triangular wave, square wave, or any irregularly changing light signal control.

[0032] In an embodiment of the present invention, the processing unit is implemented using a microcontroller system. The emission intensity of the light emitter is controlled by a DAC-controlled driving light-emitting circuit to simulate a simulated fluorescence signal with variable light intensity.

[0033] Combination Figure 2 As shown, the calibration device is equipped with an optical signal receiving circuit, including a photodiode and an operational amplifier circuit. The photodiode is used to perform photoelectric conversion on the optical signal received from the optical signal receiving interface to obtain an electrical signal, and the operational amplifier circuit generates a square wave signal, which serves as a synchronization signal for triggering the processing unit. After receiving the square wave signal, the processing unit outputs an analog fluorescent electrical signal through the DAC interface at the end of the square wave signal. The signal is then driven by the light-emitting control circuit to generate an analog fluorescent signal, which is then guided by an optical fiber to the fluorescent temperature demodulator for analysis.

[0034] {Example 2} Combination Figure 4 As shown, the calibration system design for the fluorescence thermometer demodulator in the above embodiments includes the following steps in its calibration process: After the system is powered on, the fluorescent temperature demodulator sends light pulses at preset time intervals. The light pulses are transmitted into the calibration device through the optical fiber beam guide. The optical signal receiving circuit of the calibration device converts them into square wave trigger signals and transmits them to the processing unit. The processing unit generates an analog electrical signal with a preset function according to the end position of the square wave signal through the DAC interface. After being amplified by the light emission control circuit, the signal drives the light emitter to emit light, converting the analog electrical signal into an analog fluorescent signal, which is then transmitted into the fluorescent temperature demodulator through the fiber optic beam guide. The fluorescence temperature demodulator analyzes the received analog fluorescence signal to obtain the corresponding fluorescence lifetime value and the signal intensity of the received optical signal, and feeds it back to the calibration device through serial communication. The calibration device compares the returned signal strength with the preset adaptation range, and generates a light intensity adjustment command output through the DAC control of the processing unit to adjust the light intensity of the light emitter, forming a closed-loop regulation of repetitive signal generation and intensity feedback until the signal strength is stable; After the signal strength stabilizes, the calibration device sends the standard fluorescence lifetime value and calibration command to the fluorescence temperature demodulator via serial communication. The fluorescence temperature demodulator stores the difference between the obtained fluorescence lifetime value and the standard fluorescence lifetime value as a calibration value based on the received calibration command. This value is used to compensate for the fluorescence temperature demodulator during actual temperature detection.

[0035] {Example 3} In one specific embodiment, we combine Figure 2 , 3 As shown, the logic and process of the aforementioned calibration method will be further explained.

[0036] Combination Figure 2 , Figure 4 After the calibration system is powered on, it initializes. The microcontroller system loads a preset program, which, in conjunction with the aforementioned embodiments, includes preset function waveform parameters, signal adaptation intensity ranges, temperature-fluorescence lifetime mapping tables, etc. The demodulator enters a signal transmission-reception ready state, and the serial port display initializes and indicates that calibration is ready. The system performs timing synchronization and parameter presets in its initial state, providing a foundation for calibration.

[0037] After initialization, the demodulator automatically sends the optical pulse signal (trigger signal) required for fluorescent fiber optic temperature measurement at preset intervals (e.g., 10ms / time), triggering the calibration device to synchronize. The optical pulse is transmitted through the first branch of the fiber optic guide beam to the optical signal receiving interface of the calibration device and is received by the internal photodiode. The photodiode converts the optical pulse into an electrical signal through the photoelectric effect. After being amplified by the operational amplifier circuit of the optical signal receiving circuit and filtered to remove clutter, a square wave trigger signal with clear edges is formed. The square wave trigger signal is transmitted to the I / O trigger interface of the microcontroller system through the control circuit. After the microcontroller system recognizes the signal, it confirms that the demodulator is ready and starts the analog signal generation to achieve timing synchronization between the calibration device and the demodulator.

[0038] Then, at the timing node where the square wave trigger signal ends, the microcontroller system of the calibration device calls the temperature-fluorescence lifetime mapping table according to the preset target calibration temperature to determine the corresponding fluorescence lifetime parameter. The microcontroller system generates a discrete digital sequence according to the exponential decay function (this example uses the exponential decay function as an example, but it is not a limitation, but is used to match the decay law of the real fluorescence signal). The digital sequence is converted into a continuous analog electrical signal through the DAC interface. The analog electrical signal is transmitted to the light emission control circuit, and after power amplification (adapting to the laser tube / LED driving requirements), it drives the laser tube / LED to convert the electrical signal into an optical signal, simulating the output of the fluorescence optical signal. Then, it is collimated into parallel light by the first collimating lens and transmitted to the photoelectric receiving module of the demodulator through the second branch of the optical fiber guide beam.

[0039] Therefore, by using a simulated fluorescence light signal with precisely controllable parameters to replace the real fluorescence signal of the sensor in traditional calibration, a standard and traceable calibration signal source is provided for the demodulator. This eliminates the dependence on constant temperature oil baths and sensors, enabling individual calibration of the demodulator, correcting the comprehensive errors of the optical path and circuit connected to the sensor, achieving consistency of the sensor interface, and thus achieving rapid calibration and saving in terms of production capacity, manpower, and material resources.

[0040] During the calibration process, the microcontroller system compares the received signal strength value with a preset adaptation range (e.g., 100-180) to determine the signal status. For example: Signal strength < 100 (too weak): Generate a command to increase drive current; Signal strength > 180 (too strong): Generate a command to reduce drive current; Signal strength between 100-180 (adapted): Generate a command to maintain the current.

[0041] Accordingly, the adjustment command is transmitted to the light-emitting control circuit through the control circuit. The light-emitting control circuit changes the output current, thereby adjusting the light-emitting power of the laser tube / LED emitter. The greater the current, the stronger the light intensity.

[0042] The regulated optical signal is transmitted again to the demodulator via optical fiber. The demodulator repeats the parsing-feedback operation, and the microcontroller system continuously compares the signal strength until the signal stabilizes within the adaptation range, for example, by determining that the fluctuation of three consecutive feedback values ​​is ≤±5. Thus, closed-loop feedback stabilizes the optical signal strength received by the demodulator.

[0043] Once the intensity of the optical signal fed back by the demodulator stabilizes, the microcontroller system confirms that the calibration conditions are met and extracts the standard fluorescence lifetime value corresponding to the analog signal from the preset parameters. The microcontroller system then sends the calibration command and the standard fluorescence lifetime value to the demodulator via the serial communication module.

[0044] After receiving the command, the demodulator calculates the difference between its own resolved fluorescence lifetime value and the standard value to obtain the channel error calibration value. The calibration value = standard value - resolved value.

[0045] The demodulator stores the standard fluorescence lifetime value, resolution value, and error calibration value together in its internal memory, preferably non-volatile memory such as EEPROM / flash, to ensure that the data is not lost when power is off.

[0046] Therefore, the inherent error of this channel of the demodulator is recorded, providing a calibration value for error compensation during subsequent actual temperature measurement, thus completing the individual calibration of the demodulator.

[0047] Furthermore, after the demodulator finishes storing the data, it sends a calibration completion confirmation signal to the calibration device via a serial port. After receiving the confirmation signal, the microcontroller system sends the calibration result to the serial port display via a serial port, and the display shows the calibration result intuitively. After the operator confirms the calibration result, they can choose to continue calibrating the next channel or stop the calibration, thereby completing the single-channel / multi-channel calibration process.

[0048] While the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the invention. Those skilled in the art can make various modifications and refinements without departing from the spirit and scope of the invention. Therefore, the scope of protection of the present invention shall be determined by the claims.

Claims

1. A calibration system for a fluorescence thermometer demodulator, characterized in that, This includes a calibrated fluorescence temperature demodulator, a calibration device, and an optical fiber beamguide; The fiber optic beam has a merging end and a branching end. The merging end is connected to the fiber optic interface of the fluorescence temperature demodulator, and the two branching ends are respectively connected to the optical signal transmitting interface and the optical signal receiving interface of the calibration device. The fluorescence temperature demodulator is configured to send optical pulses at preset time intervals after power-on. The pulses enter the calibration device through its optical fiber interface, the merging end of the optical fiber guide beam, the first branch end of the optical fiber guide beam, and the optical signal receiving interface. The calibration device performs synchronous control accordingly and triggers the emission of simulated fluorescence signals based on the pulse signal. The calibration device is equipped with a processing unit and an analog fluorescence signal transmitting unit connected to the processing unit; the processing unit is configured to generate digital instructions for analog fluorescence signals, and the analog fluorescence signal transmitting unit is configured to convert analog electrical signals into optical signals according to the digital instructions of the processing unit, output analog fluorescence signals with variable light intensity according to a preset function law, and reach the fluorescence temperature demodulator in sequence through the optical signal transmitting interface, the first branch end and the merging end of the optical fiber guide beam. The fluorescence temperature demodulator is configured to analyze the received light signal to obtain the fluorescence lifetime value and calculate the intensity of the light signal, and then return the intensity signal of the light signal to the calibration device through the communication interface. The processing unit of the calibration device determines whether the fluorescence temperature demodulator has stably obtained the optical signal based on the returned optical signal, and controls the generation and transmission of the standard fluorescence lifetime value and calibration command to the fluorescence temperature demodulator in response to the stable signal strength. The fluorescence temperature demodulator stores the difference between the obtained fluorescence lifetime value and the standard fluorescence lifetime value as a calibration value according to the received calibration command, which is used for compensation of the fluorescence temperature demodulator during actual temperature detection.

2. The calibration system for a fluorescence thermometer demodulator according to claim 1, characterized in that, The calibration device has a first serial port module, which is connected to the serial port of the fluorescence temperature demodulator via a communication cable to achieve serial communication.

3. The calibration system for a fluorescence thermometer demodulator according to claim 1, characterized in that, The simulated fluorescence signal transmitting unit includes a light emission control circuit and a light emitter. The processing unit drives the light emitter to emit a simulated fluorescence signal with variable intensity by controlling the light emission control circuit. The light intensity is configured to change according to a preset function.

4. The calibration system for a fluorescence thermometer demodulator according to claim 3, characterized in that, The preset function follows a pattern that can be one of the following: sine function, exponential function, triangular wave, or square wave.

5. The calibration system for a fluorescence thermometer demodulator according to claim 3, characterized in that, The light emitter is either a laser generator or an LED light emitter.

6. The calibration system for a fluorescence thermometer demodulator according to claim 3, characterized in that, The processing unit is implemented using a microcontroller system. It controls the emission intensity adjustment of the light emitter by controlling the light-emitting circuit through a DAC, thereby simulating a simulated fluorescence signal with variable light intensity.

7. The calibration system for a fluorescence thermometer demodulator according to any one of claims 1-6, characterized in that, The calibration device is equipped with an optical signal receiving circuit, including a photodiode and an operational amplifier circuit. The photodiode is used to perform photoelectric conversion on the optical signal received from the optical signal receiving interface to obtain an electrical signal, and the operational amplifier circuit generates a square wave signal, which serves as a synchronization signal for triggering the processing unit. After receiving the square wave signal, the processing unit outputs an analog fluorescent electrical signal through the DAC interface at the end of the square wave signal. The signal is then driven by the light-emitting control circuit to generate an analog fluorescent signal, which is then guided by an optical fiber to the fluorescent temperature demodulator for analysis.

8. The calibration system for a fluorescence thermometer demodulator according to any one of claims 1-6, characterized in that, The calibration device is equipped with a first collimating lens and a second collimating lens. The first collimating lens is used to collimate the emitted analog fluorescence signal and then send it into the optical signal transmitting interface. The second collimating lens is used to collimate the optical signal returned from the optical signal receiving interface and then send it into the photodiode.

9. A calibration method for a fluorescence thermometer based on the calibration system for a fluorescence thermometer according to any one of claims 1-8, characterized in that, The calibration method includes the following steps: After the system is powered on, the fluorescent temperature demodulator sends light pulses at preset time intervals. The light pulses are transmitted into the calibration device through the optical fiber beam guide. The optical signal receiving circuit of the calibration device converts them into square wave trigger signals and transmits them to the processing unit. The processing unit generates a pre-defined analog electrical signal based on the end position of the square wave signal through the DAC interface. After being amplified by the light emission control circuit, the signal drives the light emitter to emit light, converting the analog electrical signal into an analog fluorescent signal. The signal is then transmitted through an optical fiber beam guide into the fluorescent temperature demodulator. The fluorescence temperature demodulator analyzes the received analog fluorescence signal to obtain the corresponding fluorescence lifetime value and the signal intensity of the received optical signal, and feeds it back to the calibration device through serial communication. The calibration device compares the returned signal strength with the preset adaptation range, and generates a light intensity adjustment command output through the DAC control of the processing unit to adjust the light intensity of the light emitter, forming a closed-loop regulation of repetitive signal generation and intensity feedback until the signal strength is stable; After the signal strength stabilizes, the calibration device sends the standard fluorescence lifetime value and calibration command to the fluorescence temperature demodulator via serial communication. The fluorescence temperature demodulator stores the difference between the obtained fluorescence lifetime value and the standard fluorescence lifetime value as a calibration value based on the received calibration command. This value is used to compensate for the fluorescence temperature demodulator during actual temperature detection.

10. The calibration method for a fluorescence thermometer demodulator according to claim 9, characterized in that, The preset function follows a pattern that can be one of the following: sine function, exponential function, triangular wave, or square wave.

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