Sample separation device and sample separation method

Abstract

A sample separation apparatus and a sample separation method, the sample separation apparatus comprising a fluid distribution assembly for communicating with a storage assembly for storing a sample having a plurality of sample layers, at least one extraction assembly, and a detection module; the extraction assembly is communicated with the fluid distribution assembly and is used for extracting one sample layer or sequentially extracting different sample layers; the detection module is arranged on an extraction pipeline between the extraction assembly and the fluid distribution assembly, and a sample layering state detector in the detection module is used for emitting a detection signal towards a sample layer flowing through the extraction pipeline and receiving the detection signal reflected by the sample layer; and the type of the sample layer flowing through the extraction pipeline is judged according to the difference value of the detection signals. The sample separation device is high in automation degree, does not depend on manual visual layering, can accurately extract layered liquid of different layers, improves the recovery purity and recovery rate of different sample layers, and can perform quantitative split charging.

Description

Technical Field

[0001] This application relates to the field of sample processing technology, and in particular to a sample separation device and a sample separation method. Background Technology

[0002] To facilitate accurate detection of specific indicators and treatment, whole blood is typically separated into three layers by centrifugation. Centrifugation is based on the density differences of blood components. The three layers are: an uppermost pale yellow, semi-transparent layer of anemic platelet-rich plasma; a middle, thin, grayish-white layer of platelet-rich plasma; and a lowermost, deep red layer of red blood cells. In various applications, blood products located in different layers need to be transferred and extracted for subsequent biochemical uses, such as clinical testing or component transfusion.

[0003] Currently, the separation of blood is mainly done manually by visual inspection, which has low accuracy. When extracting a certain layer, components from other layers are easily mixed in, resulting in low efficiency and loss of blood components from each layer. Summary of the Invention

[0004] In view of this, in order to solve at least one of the above technical problems, it is necessary to propose a sample separation device and a sample separation method.

[0005] In a first aspect, this application provides a sample separation apparatus, comprising: a fluid distribution component, at least one extraction component, and a detection module, wherein the fluid distribution component is configured to communicate with a storage component for storing a sample having multiple sample layers; the extraction component is configured to communicate with the fluid distribution component for extracting one sample layer from the storage component, or for sequentially extracting different sample layers from the storage component; the detection module is disposed on an extraction conduit between the extraction component and the fluid distribution component, and the detection module includes a sample layer status detector, which is configured to emit a detection signal toward the sample layer flowing through the extraction conduit and receive a detection signal reflected back by the sample layer, so as to determine the type of the sample layer flowing through the extraction conduit based on the difference between the emitted detection signal and the reflected detection signal.

[0006] In some possible embodiments, the detection module further includes a flow detector for detecting the flow rate of the sample layer flowing through the extraction conduit.

[0007] In some possible embodiments, the flow detector includes an ultrasonic flow sensor, wherein the direction of the ultrasonic waves emitted by the ultrasonic flow sensor is at an acute angle to the axis of the extraction conduit.

[0008] In some possible embodiments, the extraction assembly includes a power source and the extraction conduit, the power source being used to provide driving force for fluid transfer, the power source being connected to the fluid distribution assembly via the extraction conduit, or the power source being connected to a conduit on the fluid distribution assembly at an end away from the extraction conduit.

[0009] In some possible embodiments, the sample separation device further includes a storage component comprising a centrifuge and a storage container disposed on the centrifuge, the extraction component further comprising injecting the sample into the storage container, and the centrifuge comprising centrifuging and stratifying the sample in the storage container to form the plurality of sample layers.

[0010] In some possible embodiments, the sample dispensing system includes a plurality of extraction components, one of which is used to inject the sample into the storage component, and the other extraction components are used to extract the sample layer.

[0011] In some possible embodiments, the sample stratification state detector includes an infrared photoelectric sensor; and / or, the sample separation device further includes a user interaction module for displaying dispensing data and enabling user interaction.

[0012] Secondly, embodiments of this application provide a sample separation method, comprising: separating and recovering multiple sample layers, wherein, during the process of separating and recovering each of the sample layers, a sample layer status detector transmits a detection signal toward the sample layer flowing through the extraction pipeline and receives a detection signal reflected back by the sample layer, so as to determine the type of the sample layer flowing through the extraction pipeline based on the difference between the transmitted detection signal and the reflected detection signal.

[0013] In some possible embodiments, during the separation of each of the sample layers, the method further includes detecting the flow rate of the sample layer flowing through the extraction conduit using a flow detector.

[0014] In some possible embodiments, the flow detector includes an ultrasonic flow sensor, wherein the method for detecting the flow of the sample layer includes: emitting ultrasonic waves through the ultrasonic flow sensor toward the sample layer flowing through the extraction conduit, the direction of the ultrasonic waves being at an acute angle to the axis of the extraction conduit.

[0015] In some possible embodiments, prior to the step of separating and recovering the multiple sample layers, the method further includes centrifuging the samples to form the multiple sample layers.

[0016] The sample separation device and method provided in this application integrate various multifunctional modules to achieve one-click automated sample dispensing and recovery. The high degree of automation effectively reduces human intervention, minimizes manual operation errors, saves manpower, and lowers costs. Through the design of a sample stratification status detector, it can accurately detect whether the sample flowing through the extraction pipeline has changed, eliminating reliance on manual visual stratification. It can accurately extract different layers of the stratified liquid, improving the purity and recovery rate of different sample layers and ensuring optimal detection and treatment effects. The design of a flow detector allows for real-time online detection of sample volume, accurately controlling the amount of recovered sample. Users can customize the extraction volume, and quantitative dispensing can be achieved through the flow detector. Furthermore, the entire dispensing process is carried out within a sealed pipeline, avoiding environmental pollution and cross-contamination between samples, thus improving the quality of the dispensed samples. Attached Figure Description

[0017] Figure 1 This is a hardware architecture diagram of a sample separation device provided in an embodiment of this application.

[0018] Figure 2 This is a comparison image of whole blood before and after stratification.

[0019] Figure 3 This is a schematic diagram of the sample separation device provided in an embodiment of this application.

[0020] Figure 4 for Figure 3 A schematic diagram of the structure of the sample separation device for separating different sample layers.

[0021] Figure 5 This is a schematic diagram of the structure of a sample separation device provided in another embodiment of this application.

[0022] Figure 6 This is a schematic diagram illustrating the process by which a sample stratification state detector, provided in an embodiment of this application, detects different blood layers flowing through an extraction tube.

[0023] Figure 7 This is a schematic diagram of a flow detector used in an embodiment of this application to detect the flow rate of a sample flowing through an extraction pipeline.

[0024] Figure 8 This is a schematic diagram of the sample separation device provided in another embodiment of this application.

[0025] Figure 9 A flowchart of a sample separation method provided in an embodiment of this application.

[0026] Explanation of main component symbols Sample separation devices 100, 200; storage component 1; storage container 11; main pipeline 12; centrifuge 13; fluid distribution component 2; first orifice 21; second orifice 22; extraction components 3, 3-1, 3-2, 3-3, 3-4; extraction tubing 31; power source 32; drive mechanism 33; syringe 34; detection module 4; sample stratification status detector 5; transmitter 51; receiver 52; flow detector 6; controller 7; user interaction module 8; direction X; axis a.

[0027] The following detailed description, in conjunction with the accompanying drawings, will further illustrate this application. Detailed Implementation

[0028] The technical solutions in the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments.

[0029] It should be noted that when a component is described as "fixed to" or "mounted to" another component, it can be directly on the other component or may be interspersed with an intermediate component. When a component is described as "set to" another component, it can be directly set on the other component or may be interspersed with an intermediate component. The term "and / or" as used herein includes all and any combination of one or more of the associated listed items.

[0030] It should be noted that although a logical order is shown in the flowchart, in some cases, the steps shown or described may be performed in a different order than that shown in the flowchart. The methods disclosed in the embodiments of this application include one or more steps or actions for implementing the method. Method steps and / or actions may be interchanged with each other without departing from the scope of the claims. Unless a specific order of steps or actions is specified, the order and / or use of specific steps and / or actions may be modified without departing from the scope of the claims.

[0031] Please see Figure 1 As shown, one embodiment of this application provides a sample separation device 100, which can be used for the separation of biochemical samples, for example, but not limited to, the separation of blood samples, separating different layers of the stratified blood sample (from top to bottom: anemic platelet-rich plasma layer, platelet-rich plasma layer, and red blood cell layer, etc.). Figure 2 (As shown) are packaged for subsequent biochemical applications.

[0032] Please see Figures 1 to 3As shown, the sample separation device 100 includes: a storage component 1, a fluid distribution component 2, at least one extraction component 3, and a detection module 4. The storage component 1 stores the stratified sample, which includes multiple sample layers. The extraction component 3 is connected to the storage component 1 via the fluid distribution component 2 and is used to extract the sample layers from the storage component 1. The detection module 4 is disposed on an extraction conduit 31 between the extraction component 3 and the fluid distribution component 2. The detection module 4 includes a sample stratification status detector 5, which emits detection signals toward different sample layers flowing through the extraction conduit 31 and receives detection signals reflected back by the sample layers. The detector determines the type of sample layer flowing through the extraction conduit 31 based on the difference between the emitted and reflected detection signals. When the detected sample belongs to the same layer type, the extraction component 3 can continue extraction and packaging; when the detected sample type changes, the extraction component 3 stops extraction, thereby achieving precise packaging of different sample layers. In addition, the sample separation device 100 also includes a controller 7, which can control the coordinated operation of the fluid dispensing component 2, the extraction component 3 and the detection module 4 to achieve automated sample separation.

[0033] The storage component 1 includes a storage container 11 in which a sample (e.g., whole blood) is placed after being processed into layers. The storage container 11 can be connected to the fluid distribution component 2 via a main pipeline 12. It is understood that the storage component 1 may not be part of the sample separation device 100. When sample layers need to be separated, the fluid distribution component 2 is connected to the storage container 11 via the main pipeline 12, and the corresponding sample layer can be extracted by inserting a sip needle from the main pipeline 12 into the storage container 11. It is also understood that the sip needle on the main pipeline 12 can be controlled by a robotic arm to change the extraction position, thereby uniformly extracting the same sample layer.

[0034] In some embodiments, the storage component 1 may include a centrifuge 13, and the storage container 11 may be rotatably mounted on the centrifuge 13. The centrifuge 13 separates the sample in the storage container 11 into layers. That is, the storage component 1 itself is a centrifuge device, and the centrifuge device can be directly connected to the sample separation device 100. This eliminates the need to move the separated sample layers to avoid shaking and affecting the separation effect.

[0035] When the storage component 1 is capable of centrifuging and separating samples, the extraction component 3 can also be used to inject samples into the storage component 1, specifically into the storage container 11. After injecting samples into the storage container 11 via the extraction component 3, the centrifuge 13 can be started to centrifuge and separate the samples in the storage container 11. This design simplifies the complexity of the liquid circuit connections, simplifies the device structure, and reduces the difficulty of operation.

[0036] Please see Figure 3 As shown, the fluid distribution component 2 is used to select the flow direction of the sample, enabling switching between different fluid paths. The fluid distribution component 2 includes multiple orifices, specifically a first orifice 21 and a second orifice 22. The storage component 1 is connected to the first orifice 21 via a main pipeline 12, and the extraction component 3 is connected to the second orifice 22 via an extraction pipeline 31. Multiple second orifices 22 can be provided depending on the number of extraction components 3. The sample transport direction can be selected by choosing which orifice to open.

[0037] In some embodiments, the fluid distribution assembly 2 may be an electrically operated rotary valve, the rotation of which can be controlled by a controller 7 to open the corresponding orifice. The fluid distribution assembly 2 may also be a solenoid valve, a pinch valve, or other device for controlling the opening and closing of pipelines.

[0038] Please see Figure 3 As shown, the extraction component 3 includes a power source 32 and an extraction pipeline 31. The power source 32 is used to provide driving force for fluid transfer, and the power source 32 is connected to the fluid distribution component 2 through the extraction pipeline 31.

[0039] In some embodiments, the power source 32 may be an injection pump, including a drive mechanism 33 and a syringe 34. The drive mechanism 33 can drive the injection and aspiration actions of the syringe 34 to provide power for sample transfer.

[0040] In some embodiments, the drive mechanism 33 may be a screw motor.

[0041] Please see Figures 3 to 5 As shown, the sample separation device 100 may include one or more extraction components 3. When one extraction component 3 is provided, it can be used to inject the sample or to extract different sample layers sequentially from top to bottom. When multiple extraction components 3 are provided, one extraction component 3 is used for sample injection, and the other extraction components 3 are used to recover the extracted sample layers. For example, for the aliquoting of three components of blood, the sample separation device 100 may include 1 to 4 extraction components 3.

[0042] like Figure 3As shown, the sample separation device 100 can be equipped with four extraction components 3, one extraction component 3-1 for injecting samples into the storage component 1, and the other three extraction components (3-2, 3-3, and 3-4) for extracting one sample layer from it. Figure 4 As shown, specifically, the sample is first injected into the storage container 11 using extraction component 3-1. Then, the centrifuge 13 is started, separating the sample in the storage container 11 into three layers. Afterward, extraction components 3-2, 3-3, and 3-4 are started sequentially, extracting and recovering the three sample layers from top to bottom. Additionally, the extraction components (3-2, 3-3, and 3-4) can be connected to a recovery container (not shown), allowing the extracted sample layers to be transferred to their respective recovery containers. By separating sample injection and recovery, and simultaneously separating the recovery of different layers, the separation purity of a single sample layer can be improved, preventing the separated sample layer from being contaminated with components from other layers, thus avoiding a decrease in separation purity.

[0043] like Figure 5 As shown, the sample separation device 100 can also be equipped with only one extraction component 3. This extraction component 3 is used to inject samples into the storage component 1 and to separate and extract three different sample layers sequentially. Specifically, the extraction component 3 is first used to inject samples into the storage container 11. Then, the centrifuge 13 is started, causing the samples in the storage container 11 to separate into three layers. The extraction component 3 is then used to separate and extract the three sample layers sequentially from the storage container 11. In this case, the extraction component 3 also needs to be connected to different recovery containers (not shown). After separating one sample layer, it needs to be injected into the corresponding recovery container for subsequent sample layer separation. This design simplifies the complexity of the device and the fluid circuit connections, reduces the number of components, and lowers costs.

[0044] Understandably, the number of extraction components 3 can also be two or three. One extraction component 3 can be used for sample injection, while another one or two extraction components 3 are used for recovery. In this case, depending on the purpose of separation and recovery, it is also possible to select which extraction components 3 are used to recover which sample layers. For example, for relatively rare and precious sample layers (such as the middle platelet-rich plasma layer), a single extraction component 3 can be used for recovery to improve the recovery purity.

[0045] Please see Figure 1 and Figure 3As shown, the sample layering state detector 5 determines the composition of the layered liquid by detecting the absorbance of an optical sensor, such as an infrared photoelectric sensor. The sample layering state detector 5 includes a transmitter 51 and a receiver 52, located on opposite sides of the extraction tube 31. The transmitter 51 emits a light signal towards the receiver 52, and the infrared photoelectric sensor converts the intensity of the received light signal into an output detection voltage V. When the transmitter 51 emits a light signal towards the receiver 52, the light signal passes through the extraction tube 31. When the composition of the sample layer flowing through the extraction tube 31 is different (e.g., the three components in blood), the light signal received by the receiver 52 will change (the received signal intensity will decrease) because different components have different absorption values ​​for the light signal, thus changing the output voltage.

[0046] Combination Figure 2 and Figure 6 As shown, when the extraction tube 31 is not flowing with a sample, it is empty, and the output detection voltage is V0. When the extraction tube 31 flows through the first layer of anemic platelet plasma, the output detection voltage is V1. When the extraction tube 31 flows through the second layer of platelet-rich plasma, the output detection voltage is V2. When the extraction tube 31 flows through the third layer of red blood cells, the output detection voltage is V3. Because the absorption of light signals by air, the anemic platelet plasma layer, the platelet-rich plasma layer, and the red blood cell layer is different, the values ​​of detection voltages V0, V1, V2, and V3 are different, which is used to distinguish different sample layers. When a change in the detected voltage value is detected, for example, when recovering the anemic platelet plasma layer, the process stops after detecting the platelet-rich plasma layer, indicating that the anemic platelet plasma layer has been completely recovered. This process is repeated, so that each recovery can accurately recover the sample of a single sample layer.

[0047] In some embodiments, the direction of the light signal emitted by the transmitting end 51 is perpendicular to the flow direction of the sample in the extraction component 3.

[0048] Please see Figure 1 and Figure 3 As shown, the detection module 4 also includes a flow detector 6, which is used to detect the flow rate of the sample flowing through the extraction pipeline 31.

[0049] In some embodiments, such as Figure 7As shown, the flow detector 6 includes an ultrasonic flow sensor. The direction X of the ultrasonic waves emitted by the ultrasonic flow sensor forms an acute angle α with the axis a of the extraction pipe 31. The ultrasonic flow sensor uses ultrasonic time-of-flight (TOF) technology, which is the most accurate method in non-invasive flow measurement solutions. The ultrasonic waves, in the direction X, enter the pipe at a certain angle relative to the axis a of the extraction pipe 31 and propagate in the liquid. The propagation time in the direction opposite to the liquid flow is always greater than the propagation time in the same direction as the liquid flow. The time difference in the two directions is proportional to the liquid flow velocity. Using this information, the volumetric flow velocity can be determined, and thus the corresponding flow rate can be obtained. Therefore, the flow detector 6 can monitor the liquid flow rate in this liquid path. When the user wants to accurately recover a fixed volume or accurately inject a fixed volume, the flow detector 6 can be used to control the recovery and injection volume.

[0050] Please see Figure 3 As shown, the detection module 4 is located at the end of the extraction tube 31 away from the fluid distribution component 2, that is, at the end of the injection syringe 34, so that the same sample layer can be recovered more fully.

[0051] Please see Figure 1 and Figure 3 As shown, the sample separation device 100 also includes a user interaction module 8, used to display corresponding dispensing data and enable user interaction. It also allows operators to perform dispensing operations and monitor the progress of the dispensing process, enabling timely manual intervention in case of abnormalities. The dispensing data may include pre-inputted dispensing information and dispensing process information. Dispensing information may include, for example, which sample layer to dispense, the number of dispensing portions, and the dispensing volume. Dispensing process information may include, for example, the detection voltage output by the sample layer status detector 5, the liquid flow rate output by the flow detector 6, and the connection port status of the fluid distribution component 2.

[0052] In some embodiments, the user interaction module 8 may include a display and a keyboard, etc.

[0053] Please see Figure 8 As shown, this application provides another sample separation device 200, which is basically the same as the aforementioned sample separation device 100. The main difference is that the sample separation device 100 includes a storage component 1, a fluid distribution component 2, an extraction component 3a, and a detection module 4. The storage component 1, the fluid distribution component 2, and the detection module 4 are all basically the same as those in the aforementioned embodiment. Please refer to the aforementioned embodiment, and they will not be described in detail here.

[0054] In the sample separation device 100, the extraction component 3a includes a power source 32a and at least one extraction pipeline 31, wherein the power source 3a is connected to the pipeline between the storage component 1 and the fluid distribution component 2, that is, the power source 3a is connected to the main pipeline 12 to provide driving force for sample transfer.

[0055] In some embodiments, the power source 3a may be a peristaltic pump.

[0056] In some embodiments, the number of extraction pipelines 31 can be one or more. The specific injection and recovery logic is basically the same as the injection and recovery logic containing one or more extraction components in the foregoing embodiments. Please refer to the foregoing embodiments for details, which will not be elaborated here.

[0057] In some embodiments, the end of the extraction conduit 31 away from the fluid distribution component 2 can be connected to a sample container (not shown), and the sample can be injected into the storage component 1 by the drive of the power source 3a; it can also be connected to a recovery container (not shown), and different sample layers can be extracted into the corresponding recovery containers by the drive of the power source 3a.

[0058] Please see Figure 9 As shown, refer to the following: Figures 1 to 5 As shown, this application embodiment provides a sample separation method using the sample separation device 100, the specific process including the following steps: Step S1, Sample injection: The sample to be dispensed (e.g., whole blood) is injected into storage component 1.

[0059] When only one extraction component 3 is set, the sample to be dispensed is first extracted from the syringe 34 of the extraction component 3, and then the sample to be dispensed in the syringe 34 is injected into the storage container 11 through the extraction tube 31 and the fluid dispensing component 2 by the drive mechanism 33.

[0060] When multiple extraction components are set (including 3-1, 3-2, 3-3 and 3-4), the sample to be dispensed is injected into the storage container 11 in the same way using extraction component 3-1.

[0061] Step S2, Layering: The samples to be packaged in storage component 1 are layered to form multiple sample layers.

[0062] The centrifuge 13 in the storage component 1 is used to centrifuge and separate the sample to be dispensed in the storage container 11 to form a multi-layer sample layer. For example, after whole blood is centrifuged, it will be separated into layers from top to bottom: anemic platelet plasma layer, platelet-rich plasma layer and red blood cell layer.

[0063] Steps S1 and S2 are not necessary steps. If the samples in storage component 1 are already stratified samples, then steps S1 and S2 are not required.

[0064] Step S3, stratified recovery: Separate and recover multiple sample layers.

[0065] During the separation of each sample layer, the sample layer separation status detector 5 emits a detection signal toward the sample layer flowing through the extraction conduit 31 and receives the detection signal reflected back by the sample layer. The type of sample layer flowing through the extraction conduit 31 is determined based on the difference between the emitted and reflected detection signals. Furthermore, the separation process can be controlled to stop based on whether the detected sample layer type has changed. For details on the determination method, please refer to the foregoing description.

[0066] like Figure 3 and Figure 4 As shown, the process for sample recovery using multiple extraction components is as follows: extraction components 3-2, 3-3, and 3-4 are activated sequentially, extracting and recovering the three sample layers from top to bottom. Additionally, the extraction components (3-2, 3-3, and 3-4) can be connected to a recovery container (not shown), allowing the extracted sample layers to be transferred to the corresponding recovery container.

[0067] like Figure 5 As shown, the process of sample recovery using an extraction component 3 is as follows: The extraction component 3 first separates and extracts the first layer of the three sample layers from the storage container 11 and transfers it to the corresponding recovery container; then, the extraction component 3 separates and extracts the second layer from the storage container 11 and transfers it to the corresponding recovery container; finally, the extraction component 3 separates and extracts the third layer from the storage container 11 and transfers it to the corresponding recovery container. The extraction and recovery operation can be repeated using the extraction component 3 depending on the specific number of sample layers.

[0068] In some embodiments, step S3 further includes: detecting the flow rate of the sample layer flowing through the extraction pipeline 31 using a flow detector 6, thereby achieving quantitative dispensing. For specific quantitative methods and principles, please refer to the foregoing content; further details will not be elaborated here.

[0069] In addition, the method of sample separation using the aforementioned sample separation device 200 is basically the same as the method of sample separation using the sample separation device 100, but there are some differences in the driving method due to the different positions of the power source 3a.

[0070] In summary, the sample separation device 100 (200) and sample separation method provided in this application embodiment combine various multifunctional modules to achieve one-click automated sample dispensing and recovery process. The high degree of automation effectively reduces human intervention, reduces human operation errors, saves manpower, and reduces costs. Through the design of the sample stratification state detector 5, it can accurately identify whether the sample flowing through the extraction tube 31 has changed, without relying on manual visual stratification, and can accurately extract the stratified liquid of different layers, improve the recovery purity and recovery rate of different sample layers, and ensure the best detection and treatment effect. Through the design of the flow detector 6, it can detect the sample volume online in real time, accurately control the amount of recovered sample, and the user can customize the extraction volume. Through the quantitative measurement of the flow detector 6, the purpose of quantitative dispensing can be achieved. In addition, the entire dispensing process is carried out in a sealed pipeline, avoiding environmental pollution and cross-contamination between samples, and improving the quality of the dispensed samples.

[0071] In addition, by configuring one or more extraction components 3 in the device, the flexibility of sample recovery can be improved.

[0072] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application and are not intended to limit it. Although this application has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of this application without departing from the spirit and scope of the technical solutions of this application.

Claims

1. A sample separation device, characterized in that, include: The system comprises a fluid dispensing component, at least one extraction component, and a detection module. The fluid distribution component is used to communicate with the storage component, the storage component is used to store samples, and the samples have multiple sample layers; The extraction component is connected to the fluid distribution component and is used to extract one sample layer from the storage component, or to extract different sample layers from the storage component sequentially. The detection module is disposed on the extraction pipeline between the extraction component and the fluid distribution component. The detection module includes a sample layer status detector, which is used to emit a detection signal toward the sample layer flowing through the extraction pipeline and receive the detection signal reflected back by the sample layer, so as to determine the type of the sample layer flowing through the extraction pipeline based on the difference between the emitted detection signal and the reflected detection signal.

2. The sample separation device as described in claim 1, characterized in that, The detection module further includes a flow detector, which is used to detect the flow rate of the sample layer flowing through the extraction pipeline.

3. The sample separation device as described in claim 2, characterized in that, The flow detector includes an ultrasonic flow sensor, and the direction of the ultrasonic waves emitted by the ultrasonic flow sensor is at an acute angle to the axis of the extraction pipeline.

4. The sample separation device as described in claim 1, characterized in that, The extraction assembly includes a power source and an extraction conduit. The power source provides driving force for fluid transfer. The power source is connected to the fluid distribution assembly through the extraction conduit, or the power source is connected to a conduit on the fluid distribution assembly at the end away from the extraction conduit.

5. The sample separation device as described in claim 1, characterized in that, It also includes a storage component, which includes a centrifuge and a storage container disposed on the centrifuge. The extraction component is further used to inject the sample into the storage container. The centrifuge is used to centrifuge and stratify the sample in the storage container to form the plurality of sample layers.

6. The sample separation device as described in claim 5, characterized in that, The sample dispensing system includes multiple extraction components, one of which is used to inject the sample into the storage component, and the other extraction components are used to extract the sample layer.

7. The sample separation device as described in claim 1, characterized in that, The sample stratification state detector includes an infrared photoelectric sensor; and / or The sample separation device also includes a user interaction module for displaying the dispensing data and enabling user interaction.

8. A sample separation method, characterized in that, include: Multiple sample layers were separated and recovered. In the process of separating and recovering each sample layer, a sample layer status detector emits a detection signal toward the sample layer flowing through the extraction tube and receives the detection signal reflected back by the sample layer, so as to determine the type of the sample layer flowing through the extraction tube based on the difference between the emitted detection signal and the reflected detection signal.

9. The sample separation method as described in claim 8, characterized in that, In the process of separating each of the sample layers, the method further includes: The flow rate of the sample layer flowing through the extraction pipeline is detected by a flow detector.

10. The sample separation method as described in claim 9, characterized in that, The flow detector includes an ultrasonic flow sensor, wherein the method for detecting the flow in the sample layer includes: The ultrasonic flow sensor emits ultrasonic waves toward the sample layer flowing through the extraction conduit, the direction of which is at an acute angle to the axis of the extraction conduit; and / or Prior to the step of separating and recovering multiple sample layers, the method further includes: The samples are centrifuged to form the plurality of sample layers.

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