A plant resistance inducer and its application in the control of fruit tree diseases

Abstract

This invention relates to a plant antagonist and its application. The main active ingredient of the plant antagonist is methyl ferulic acid, with an effective concentration of 0.05 μg / mL to 0.5 μg / mL. This invention also discloses the application of this plant antagonist in the control of diseases in fruit trees and fruits, including apples, pears, grapes, and tomatoes, and diseases including ring rot, anthracnose, and gray mold. The plant antagonist of this invention can comprehensively activate the plant immune system, simultaneously inducing a significant increase in the activity of multiple disease resistance-related enzymes (such as peroxidase, polyphenol oxidase, and glutathione S-transferase) in the plant, and upregulating multiple disease resistance-related PR genes (such as...). MdPR1 , MdPR2 The expression of [various substances] systematically enhances the innate immunity and acquired resistance of plants, achieving multi-pathway synergistic defense. This inducer works by stimulating the plant's own disease resistance mechanisms, reducing dependence on chemical pesticides and lowering the risk of pesticide residues, which aligns with the direction of green and sustainable agricultural development.

Description

Technical Field

[0001] This invention relates to the fields of agricultural biotechnology and plant protection technology, and in particular to a plant inducer and its application in the prevention and control of fruit tree diseases. Background Technology

[0002] In modern agricultural production, diseases of fruit trees, such as apples, pears, and grapes, have become key factors restricting yield and quality. Taking apple ring rot as an example, the incidence rate of this disease in major apple-producing areas of northern my country can reach 30%-50%, and in severe years, the disease rate in some orchards even exceeds 80%. This not only causes the fruit to rot and lose its commercial value, but also affects the lifespan of the tree through infection of branches and trunks, resulting in yield losses for several consecutive years. Pear anthracnose spreads rapidly in hot and humid environments. Infected fruit develops brown, sunken lesions that gradually expand, causing the entire fruit to rot within 3-5 days. The pathogen can overwinter on diseased plant debris, continuing to infect the following year, creating a vicious cycle. Grape anthracnose mainly affects mature fruit, with lesions that are grayish-white in the center and accompanied by small black dots, severely affecting the appearance and taste of grapes and lowering their commercial grade. Tomato gray mold is particularly prevalent in greenhouse cultivation. The pathogen can infect through petals and leaves, causing brown soft rot in the fruit. In continuously cropped fields, the incidence rate can reach over 60%, causing huge economic losses to farmers. While traditional chemical pesticides can suppress diseases in the short term, long-term use can lead to increased resistance in pathogens, resulting in a decline in control effectiveness year by year. At the same time, pesticide residues seriously affect the safety of agricultural products and the health of consumers, which is in significant conflict with the current needs of green agricultural development.

[0003] With tightening environmental policies and the popularization of green agriculture concepts, plant inducers are gradually becoming an important alternative to chemical pesticides due to their advantages such as environmental friendliness and low likelihood of inducing resistance. Currently commercially available inducers, such as chitosan and oligosaccharides, have achieved some success in controlling diseases of grain crops such as rice sheath blight and wheat rust, but significant technical bottlenecks remain in the control of fruit diseases, making it difficult to meet actual production needs.

[0004] The primary problem is the poor stability of the control effect. The effectiveness of existing resistance inducers is easily affected by environmental conditions and fruit varieties. Taking chitosan as an example, in the control of apple ring rot, its efficacy can reach more than 60% only under suitable conditions of relative humidity below 60% and temperature of 20-25℃. If there is continuous rainy weather (relative humidity > 80%) or temperature above 30℃, the efficacy will drop sharply to below 30%, and it cannot form a stable disease control effect. The difference in efficacy is also significant when targeting different fruit varieties. Oligosaccharides have an efficacy of about 55% against anthracnose on Fuji apples, but only 32% against Golden Delicious apples, indicating extremely poor variety compatibility. Secondly, their application lacks versatility. Due to their molecular structure, some inducers cannot penetrate the waxy and cuticle layers of the fruit epidermis on their own and require specific penetration enhancers (such as organosilicon adjuvants) to be effective. However, the epidermal structures of different fruits vary greatly (e.g., grapes have a thick waxy layer, while tomatoes have a thin and hairy epidermis). This means that the same inducer needs to be combined with different adjuvants to be applied to different fruits, which not only increases the complexity of operations and the cost of pesticides for farmers, but may also cause phytotoxicity to the fruit due to improper adjuvant selection (e.g., some adjuvants can cause spots on the grape skin). In addition, the mechanisms of action of existing inducers are relatively simple, mostly activating only one type of defense response in plants (e.g., only inducing an increase in polyphenol oxidase activity), which is insufficient to address the complex infection mechanisms of fruit diseases, resulting in poor overall control efficacy.

[0005] Therefore, developing a new type of plant inducer for fruit diseases that is stable in effect, widely adaptable to different varieties, requires no special adjuvants, and can fully activate the plant's defense system has become a key need to overcome the current dilemma of green prevention and control of fruit diseases. Summary of the Invention

[0006] The present invention aims to solve the above problems and provides a plant resistance inducer, wherein the main active ingredient of the plant resistance inducer is methyl ferulic acid, and the effective concentration of methyl ferulic acid is 0.05 μg / mL to 0.5 μg / mL.

[0007] More preferably, the effective concentration of the methyl ferulic acid is 0.1 μg / mL.

[0008] Based on the same invention, this invention also provides the application of the plant inducers described above in the prevention and control of fruit tree diseases, characterized in that the fruit trees include apple, pear and grape, and the diseases include ring rot, anthracnose leaf blight and gray mold.

[0009] Based on the same invention, this invention also provides the application of the plant inducer as described above in the prevention and control of plant fruit diseases.

[0010] Furthermore, the plant fruits include pome fruits, berries, drupes, and citrus fruits.

[0011] Furthermore, the plant fruits include apples, pears, grapes, and tomatoes.

[0012] More preferably, the apple can be the fruit or apple tree (sapling) of varieties such as 'Fuji', 'Golden Delicious', and 'Gala'; the pear can be the fruit of varieties such as 'Crown'; the grape can be the fruit of varieties such as 'Kyoho'; and the tomato can be the fruit of varieties such as 'Millennium'.

[0013] Furthermore, the diseases include ring rot, anthracnose, and gray mold. Ring rot is caused by *Aureobasidium anthracnose*, anthracnose by *Aureobasidium argentea*, and gray mold by *Aureobasidium grayi*.

[0014] Furthermore, the method of using the plant inducer includes the following steps: S1: Rinse the fruit or plant tissue with sterile water and then air dry; S2: Apply the inducer to the surface of the fruit or plant tissue by dripping or spraying; S3: After application, the cells are kept moist and incubated for 48 hours to complete the induction treatment.

[0015] Furthermore, the amount of the inducer applied is 20-50 μL / unit.

[0016] Furthermore, the inducer is used at a dosage of 50 μL / fruit in pear-type fruits and 20 μL / fruit in berry-type fruits.

[0017] Furthermore, the plant inducer exerts its preventive effect by activating the plant's own immune system, specifically including: (1) Methyl ferulic acid induces an increase in the activity of peroxidase, polyphenol oxidase and glutathione-S-transferase in plants; (2) Methyl ferulic acid induces the upregulation of disease resistance-related PR genes in plants, wherein the PR genes include MdPR1 , MdPR2 , MdPR4 , MdPR5 , MdPR8 , MdPR10 .

[0018] The present invention has the following beneficial effects: 1. The control effect of the present invention is significant and stable. Methyl ferulic acid can effectively inhibit the invasion of pathogens of fruit trees such as apple, pear and grape at extremely low concentrations (0.05–0.5 μg / mL). In addition, methyl ferulic acid also has excellent control effect on plant fruits at extremely low concentrations (0.05–0.5 μg / mL), and the effect is less affected by environmental conditions and the stability is better than existing inducers.

[0019] 2. Strong adaptability to various varieties, no auxiliary agents required: This inducer is suitable for various fruit types such as pears, berries, stone fruits, and citrus fruits. It has good control adaptability to different fruit varieties (such as 'Fuji', 'Golden Delicious', 'Kyoho', 'Millennium', etc.) and can penetrate the fruit epidermis without the need for a penetrating agent, reducing the complexity and cost of application.

[0020] 3. Methyl ferulic acid can fully activate the plant immune system and simultaneously induce a significant increase in the activity of multiple disease resistance-related enzymes (such as peroxidase, polyphenol oxidase, and glutathione-S-transferase) in the plant, and upregulate the expression of multiple disease resistance-related PR genes (such as MdPR1, MdPR2, etc.), systematically enhancing the plant's innate immunity and systemic acquired resistance, and achieving multi-pathway synergistic defense.

[0021] 4. This inducer works by stimulating the plant's own disease resistance mechanism, reducing dependence on chemical pesticides and lowering the risk of pesticide residues, which is in line with the direction of green and sustainable agricultural development.

[0022] 5. The inducer can be applied directly to the fruit surface by dripping or spraying. The operation is simple, requires no special equipment, is suitable for large-scale application, and has a low dosage, which effectively reduces the cost of prevention and control. Attached Figure Description

[0023] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only one embodiment of the present invention. For those skilled in the art, other embodiments can be derived from the provided drawings without creative effort.

[0024] Figure 1 Example 1: The protective effect of methyl ferulic acid against apple anthracnose leaf blight pathogens on the leaf surface; Figure 2 Example 2: The protective effect of methyl ferulic acid against fruit anthracnose in apple fruit; Figure 3 Example 3: The protective effect of methyl ferulic acid against anthracnose in pear fruit; Figure 4 Example 4: The protective effect of methyl ferulic acid against anthracnose in grape berries; Figure 5 Example 5: The protective effect of methyl ferulic acid against ring rot fungus on apple fruit; Figure 6 Example 5: The protective effect of methyl ferulic acid against ring rot fungus on apple branches and trunks; Figure 7Example 6: The protective effect of methyl ferulic acid against pear fruit rot caused by *Pyctomyces albuminosus*. Figure 8 Example 7: The protective effect of methyl ferulic acid against gray mold on tomato fruit; Figure 9 Example 8: Determination of the content of various disease resistance-related enzymes after apple leaves were treated with methyl ferulic acid; Figure 10 Example 9: Expression levels of disease resistance-related genes in apple leaves after treatment with methyl ferulic acid. Detailed Implementation

[0025] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, not all of them. The following embodiments are only for illustrative purposes and are not intended to limit the scope of the present invention in any way. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention. Unless otherwise specified, the experimental methods used in the present invention are conventional methods. Unless otherwise specified, the materials and reagents used in the present invention are commercially available. Furthermore, other terms used in the present invention, unless otherwise specified, generally have the meanings commonly understood by those skilled in the art.

[0026] Example 1: The efficacy of methyl ferulic acid against apple anthracnose leaf blight Fresh Gala apple leaves were collected, thoroughly rinsed with sterile water, and then air-dried. Using an in vitro leaf culture method, the petioles were wrapped in moistened absorbent cotton and placed in a sterile, humidified culture box. The experimental group leaves were sprayed evenly with methyl ferulic acid solutions at concentrations of 0.05 μg / mL, 0.1 μg / mL, 0.25 μg / mL, and 0.5 μg / mL, respectively, while the control group was sprayed with an equal volume of sterile water as a blank control. All treatments were cultured under the same humidified conditions for 48 h. The strain of *Anthracnose leaf blight* was cultured on liquid Czapek's medium for 3 days. After filtration through two layers of lens paper to obtain a spore suspension, the spores were collected by centrifugation at 5000 rpm for 10 minutes, rinsed with sterile water, and the spore concentration was adjusted to 1×10⁻⁶. 6 The concentration of lesions was increased to 0.1% Tween 20, and the solution was sprayed evenly onto the detached fresh leaves. The leaves were kept moist, and the number of lesions was counted and photographed after 4-6 days. The methyl ferulic acid used was a 95% purity technical grade, purchased from Maclean's Reagents.

[0027] Experimental results are as follows Figure 1As shown, the number of lesions on apple leaves treated with different concentrations of methyl ferulic acid decreased significantly with increasing concentration. The water control group (CK) had the most lesions, exceeding 150; the 0.1 μg / mL treatment group had fewer than 50 lesions, while the 0.5 μg / mL treatment group had very few lesions, almost none. This indicates that methyl ferulic acid has a significant inhibitory effect on *Hydrocotyle erythrorhizon*, the causal agent of apple anthracnose leaf blight, and the higher the concentration, the better the control effect, effectively reducing the formation of leaf lesions caused by pathogen infection.

[0028] Therefore, considering both the prevention and control effect and the economic cost, although the 0.1 μg / mL concentration has moderate efficacy, it has a high cost-effectiveness. Therefore, this concentration was used for further research in subsequent experiments.

[0029] Example 2: The efficacy of methyl ferulic acid against anthracnose in apple fruit Ripe 'Golden Delicious' apples were thoroughly rinsed with sterile water and then air-dried. For the experimental group, fruits were punctured with a 200 μL pipette tip, and then 50 μL of 0.1 μg / mL methyl ferulic acid was added. After air-drying, the fruits were placed in a sterile, humidified culture box for 48 h. Fungal cakes were then collected from the edge of fresh fruit anthracnose wild-type colonies using a 5 mm diameter punch and inoculated onto the punctured areas. The areas were kept moist, and the diameter of the lesions was measured and photographed after 5-7 days.

[0030] Experimental results are as follows Figure 2 As shown, the diameter of lesions on fruits treated with 0.1 μg / mL methyl ferulic acid solution was significantly smaller than that on apple fruits treated with sterile water. This indicates that methyl ferulic acid solution has a significant inhibitory effect on fruit anthracnose pathogens on apple fruits.

[0031] Example 3: The protective effect of methyl ferulic acid against anthracnose in pear fruit Mature 'Crown' pear fruits were collected, thoroughly rinsed with sterile water, and then air-dried. For the experimental group, fruits were treated by puncturing them with a 200 μL pipette tip, followed by the addition of 50 μL of 0.1 μg / mL methyl ferulic acid. After air-drying, the fruits were placed in a sterile, humidified culture box for 48 h. Fungal cakes were then collected from the edge of fresh fruit anthracnose wild-type colonies using a 5 mm diameter punch and inoculated onto the puncture sites. The sites were kept moist, and the diameter of the lesions was measured and photographed after 5-7 days.

[0032] Experimental results are as follows Figure 3 As shown, the diameter of lesions on pears treated with 0.1 μg / mL methyl ferulic acid solution was significantly smaller than that on pears treated with sterile water. This indicates that methyl ferulic acid solution has a significant inhibitory effect on fruit anthracnose pathogens on pears.

[0033] Example 4: The protective effect of methyl ferulic acid against anthracnose in grape berries Ripe 'Kyoho' grapes were harvested, thoroughly rinsed with sterile water, and then air-dried. For the experimental group, the grapes were punctured with a 200 μL pipette tip, and then 20 μL of 0.1 μg / mL methyl ferulic acid was added. After air-drying, the grapes were placed in a sterile, humidified culture box for 48 h. Using a 5 mm diameter punch, mycelial cakes were collected from the edge of fresh fruit anthracnose wild-type colonies and inoculated onto the punctured areas. The areas were kept moist, and the diameter of the lesions was measured and photographed after 3-5 days.

[0034] Experimental results are as follows Figure 4 As shown, the diameter of lesions on grapes treated with 0.1 μg / mL methyl ferulic acid solution was significantly smaller than that on grapes treated with sterile water. This indicates that methyl ferulic acid solution has a significant inhibitory effect on anthracnose pathogens on grape berries.

[0035] Example 5: The efficacy of methyl ferulic acid against apple ring rot fungus Ripe 'Fuji' apples were taken, rinsed thoroughly with sterile water, and then air-dried. For the experimental group, the fruits were treated with a 200 μL pipette tip to cause a puncture, and then 50 μL of 0.1 μg / mL methyl ferulic acid was added. After air-drying, the fruits were placed in a sterile humidification incubator for 48 h.

[0036] For apple branches, select 'Fuji' apple branches with a diameter of 1.5 cm. Use a 5 mm punch to extract the phloem. After removing the phloem, add 50 μL of 0.1 μg / mL methyl ferulic acid. The control group is treated with sterile water. After drying, place in a sterile humidification incubator for 48 h. Use a 5 mm punch to extract mycelial cakes from the edge of fresh wild-type strains of *Pseudomonas aeruginosa*. Inoculate these cakes onto the fruit and the puncture sites on the branches. Keep the branches moist. Measure the diameter of the lesions after 5-7 days and photograph them.

[0037] like Figure 5 As shown, the diameter of lesions on fruits treated with 0.1 μg / mL methyl ferulic acid solution was significantly smaller than that on apple fruits treated with sterile water. This indicates that methyl ferulic acid solution has a significant inhibitory effect on *Rhizoctonia solani* (ringworm) on apple fruits. Figure 6 As shown, the lesion length of apple branches treated with 0.1 μg / mL methyl ferulic acid solution was significantly smaller than that of apple branches treated with sterile water. This indicates that methyl ferulic acid solution also has a significant inhibitory effect on apple scab pathogens on apple branches.

[0038] Example 6: The efficacy of methyl ferulic acid against pear ring rot fungus Mature 'Crown' pear fruits were collected, thoroughly rinsed with sterile water, and then air-dried. For the experimental group, fruits were punctured with a 200 μL pipette tip, and then 50 μL of 0.1 μg / mL methyl ferulic acid was added. After air-drying, the fruits were placed in a sterile, humidified culture box for 48 h. Mycelial cakes were collected from the edge of fresh wild-type *Pseudomonas aeruginosa* colonies using a 5 mm diameter punch and inoculated onto the punctured sites. The sites were kept moist, and the diameter of the lesions was measured and photographed after 5-7 days.

[0039] Experimental results are as follows Figure 7 As shown, the diameter of lesions on pears treated with 0.1 μg / mL methyl ferulic acid solution was significantly smaller than that on pears treated with sterile water. This indicates that methyl ferulic acid solution has a significant inhibitory effect on pear scab pathogens.

[0040] Example 7: The efficacy of methyl ferulic acid against gray mold on tomatoes Ripe 'Millennium' tomatoes were harvested, thoroughly rinsed with sterile water, and then air-dried. For the experimental group, fruits were punctured with a 200 μL pipette tip, and then 20 μL of 0.1 μg / mL methyl ferulic acid was added. After air-drying, the fruits were placed in a sterile, humidified culture box for 48 h. Mycelial cakes were collected from the edge of fresh wild-type *Botrytis cinerea* colonies using a 5 mm diameter punch and inoculated onto the punctured sites. The sites were kept moist, and the diameter of the lesions was measured and photographed after 3-5 days.

[0041] Experimental results are as follows Figure 8 As shown, the diameter of lesions on fruits treated with 0.1 μg / mL methyl ferulic acid solution was significantly smaller than that on tomato fruits treated with sterile water. This indicates that methyl ferulic acid solution has a significant inhibitory effect on gray mold on tomato fruits.

[0042] Based on the experimental results of Examples 1-7, methyl ferulic acid has good control effects against a variety of pathogens on various fruits. Therefore, taking apple as an example, the control mechanism will be further explored.

[0043] Analysis of the systematic experimental results from Examples 1-7 shows that methyl ferulic acid exhibits significant control effects against various pathogenic fungi infecting different fruit varieties (such as apple, pear, and tomato), including *Anthracnose fungus*, *Rhizoctonia solani*, and *Botrytis cinerea*. It can significantly reduce the extent of lesion expansion and decrease the incidence and severity of diseases. Given that apple is a major economic fruit tree in my country, post-harvest diseases cause particularly significant economic losses, and methyl ferulic acid shows a more pronounced activity advantage in apple disease control, this study selected apple as a representative research object to conduct an in-depth investigation into the mechanism of action of methyl ferulic acid in controlling apple pathogenic fungi. The aim is to clarify the molecular targets and physiological and biochemical pathways of its antibacterial effect, providing theoretical support for the further development and application of this compound.

[0044] Example 8: Methyl ferulic acid induced the activity of apple disease resistance-related enzymes Fresh apple leaves were collected, thoroughly rinsed with sterile water, and then air-dried. An in vitro leaf culture method was used, with the petiole wrapped in moistened absorbent cotton and placed in a sterile, humidified culture box. The experimental group leaves were sprayed evenly with a 0.1 μg / mL methyl ferulic acid solution on both sides, while the control group was sprayed with an equal volume of sterile water as a blank control. All treatments were cultured under the same humidified conditions for 48 h before sampling. The peroxidase content in apple leaves was detected using Solarbio (Cat: BC0090), the polyphenol oxidase content using Solarbio (Cat: BC0190), and the glutathione S-transferase content using Solarbio (Cat: BC0350).

[0045] Data such as Figure 9 The results showed that the peroxidase content in apple leaves of the treated group was significantly higher than that of the control group (sterile water treatment). Plant peroxidases participate in key processes such as reactive oxygen species scavenging and cell wall lignification in plants. Increased activity of peroxidase can enhance the antioxidant capacity and structural defense capabilities of apples, helping them resist pathogen invasion. The polyphenol oxidase content in apple leaves of the treated group was significantly higher than that of the control group (sterile water treatment). Polyphenol oxidase plays an important role in the oxidation of phenolic substances in plants, disease resistance responses, and wound healing. Increased activity of polyphenol oxidase can strengthen the apple's defense response against pathogens. The glutathione S-transferase content in apple leaves of the treated group was significantly higher than that of the control group (sterile water treatment). Glutathione S-transferase participates in plant detoxification metabolism, antioxidant responses, and signal transduction processes. Increased activity of glutathione S-transferase can improve the apple's tolerance to adverse environments and pathogen toxins. Using apples as an example, this demonstrates that the activity of plant disease resistance-related enzymes increased after methyl ferulic acid treatment.

[0046] Example 9: Methyl ferulic acid treatment induces upregulation of apple disease resistance-related genes. Following the procedure in Example 8, fresh 'Gala' apple leaves were treated with 0.1 μg / mL methyl ferulic acid solution for 48 h before sampling. The samples were immediately flash-frozen in liquid nitrogen and stored at -80°C for subsequent RNA extraction. Total RNA was extracted from the apple leaves using a plant total RNA extraction kit manufactured by Acrel Biotech Co., Ltd. The integrity and purity of the RNA were assessed by agarose gel electrophoresis and a nucleic acid concentration analyzer. One μg of high-quality total RNA was used to synthesize first-strand cDNA according to the Novozymes HiScript® III RT SuperMix reverse transcription kit.

[0047] Using the above cDNA as a template, the QuantStudio® series real-time quantitative PCR system was used to perform the assay on apples. MdEFa1Using genes as internal controls, the effects of methyl ferulic acid treatment on the expression levels of a series of disease resistance-related pathological genes (PR) family members in apple leaves were systematically analyzed using the SYBR Green qPCR method, including... MdPR1 , MdPR2 , MdPR4 , MdPR5 , MdPR8 and MdPR10 Three technical replicates were set up for each reaction to ensure data reliability.

[0048] Use 2 –ΔΔCT The method involves quantitative analysis of relative gene expression levels using qRT-PCR results. The results are as follows: Figure 10 As shown, compared with the sterile water control group, treatment with 0.1 μg / mL methyl ferulic acid solution significantly induced all assays. PR Gene expression is upregulated. Among them, MdPR1 , MdPR2 As a marker gene for the salicylic acid signaling pathway, its strong activation indicates that systemic acquired resistance (SAR) is effectively triggered; MdPR4 , MdPR8 It belongs to the chitinase class, and its upregulation indicates enhanced cell wall degradation resistance mechanisms; MdPR5 (sweetenedin) and MdPR10 Increased expression of (ribonuclease analogs) further enhances the plant's molecular defense against pathogen infection. This suggests that methyl ferulic acid can be used as a novel plant immune inducer.

[0049] It is understood that those skilled in the art can make equivalent substitutions or modifications to the technical solutions and concepts of this invention, and all such substitutions or modifications should fall within the protection scope of the appended claims.

Claims

1. A plant inducer, characterized in that, The main active ingredient of the plant inducer is methyl ferulic acid, and the effective concentration of methyl ferulic acid is 0.05 μg / mL to 0.5 μg / mL.

2. The plant inducer according to claim 1, characterized in that, The effective concentration of the methyl ferulic acid is 0.1 μg / mL.

3. The application of the plant inducer according to claim 1 or 2 in the control of fruit tree diseases, characterized in that, The fruit trees include apples, pears, and grapes, and the diseases include ring rot, anthracnose leaf blight, and gray mold.

4. The application of the plant inducer as described in claim 1 or 2 in the prevention and control of plant fruit diseases.

5. The application according to claim 4, characterized in that, The plant fruits include pome fruits, berries, drupes, and citrus fruits.

6. The application according to claim 5, characterized in that, The plant fruits include apples, pears, grapes, and tomatoes.

7. The application according to claim 4, characterized in that, The diseases mentioned include ring spot, anthracnose, and gray mold.

8. The application according to claim 4, characterized in that, The method of using the plant resistance inducer includes the following steps: S1: Rinse the fruit or plant tissue with sterile water and then air dry; S2: Apply the inducer to the surface of the fruit or plant tissue by dripping or spraying; S3: After application, the cells are kept moist and incubated for 48 hours to complete the induction treatment.

9. The application according to claim 8, characterized in that, The dosage of the inducer is 20-50 μL / unit.

10. The application according to claim 3 or 4, characterized in that, The plant inducer exerts its preventive effect by activating the plant's own immune system, specifically including: (1) Methyl ferulic acid induces an increase in the activity of peroxidase, polyphenol oxidase and glutathione-S-transferase in plants; (2) Methyl ferulic acid induces the upregulation of disease resistance-related PR genes in plants, wherein the PR genes include MdPR1 , MdPR2 , MdPR4 , MdPR5 , MdPR8 , MdPR10 .

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