A material for controlling and regulating soil re-acidification in grape cultivation, its preparation method and application

Abstract

This invention discloses a soil re-acidification control and regulation material for grape cultivation, its preparation method, and its application, belonging to the field of soil remediation technology. It comprises the following raw materials in parts by weight: 20-35 parts of rapid neutralizing component, 30-45 parts of slow-release fertilizing component, 15-25 parts of activating and enhancing component, and 5-15 parts of microecological regulation component; the slow-release fertilizing component comprises the following raw materials in parts by weight: 10-15 parts of 10-30 mesh calcined bone meal, 15-20 parts of silkworm excrement fermentation product, and 5-10 parts of well-rotted organic fertilizer; the activating and enhancing component comprises the following raw materials in parts by weight: 8-10 parts of mineral-derived potassium humate, and 7-15 parts of eggshell powder treated with carbonization and steam. This invention can significantly increase soil organic matter, available phosphorus, available potassium, and beneficial microbial content, improve soil buffering capacity, strain activity retention rate and colonization rate, prolong pH stability period, and significantly reduce soil bulk density, aluminum ion content, and soil-borne disease incidence.

Description

Technical Field

[0001] This invention relates to the field of soil remediation technology, specifically to a material for controlling and regulating soil re-acidification in grape cultivation, its preparation method, and its application. Background Technology

[0002] Existing acidic soil conditioners are mainly divided into three categories: mineral-based, organic-inorganic compound, and functional additives.

[0003] For example, lime-based mineral conditioners have a core drawback: they neutralize acidity rapidly, causing a sudden increase in soil pH in a short period. Subsequently, due to nutrient loss and decreased soil buffering capacity, alkalization rebounds, while also damaging soil aggregate structure. Another example is commercially available general-purpose microbial agents (functional adjuvants), which have poor compatibility and tend to have low activity in high-temperature and dry environments, limiting soil colonization rates (less than 10%).

[0004] Based on this, the present invention designs a material for controlling and regulating soil re-acidification in grape cultivation, a preparation method, and an application to solve the above problems. Summary of the Invention

[0005] In view of the above-mentioned shortcomings of the existing technology, the present invention provides a material for controlling and regulating soil re-acidification in grape cultivation, its preparation method and application.

[0006] To achieve the above objectives, the present invention provides the following technical solution: A soil acidification control and regulation material for grape cultivation, comprising the following raw materials in parts by weight: 20-35 parts of rapid neutralizing component, 30-45 parts of slow-release fertilizing component, 15-25 parts of activating and enhancing component, and 5-15 parts of microecological regulation component; The following raw materials are used for rapid neutralization: 10-15 parts of 80-100 mesh dolomite powder, 8-10 parts of 80-100 mesh limestone powder, and 2-10 parts of calcium magnesium phosphate fertilizer. The slow-release fertilizer component consists of the following raw materials in parts by weight: 10-15 parts of calcined bone meal (10-30 mesh), 15-20 parts of silkworm excrement fermentation product, and 5-10 parts of well-rotted organic fertilizer; wherein, the preparation method of the silkworm excrement fermentation product is as follows: first, chitosan is grafted with gallic acid to obtain modified chitosan, and then silkworm excrement, modified chitosan, Lactobacillus reuteri strain LTR1318, and highly acid-resistant Acetobacter CM-067 are mixed, fermented, and then sterilized; The activating and enhancing components are made from the following raw materials in parts by weight: 8-10 parts of mineral-derived potassium humate, and 7-15 parts of eggshell powder that has been carbonized and steam-treated. The microecological regulation component is a microbial functional agent, which is a compound of *Priscilla megaterium* strain SY39, *Lactobacillus pentosus* BNPV, *NXT28* rhizosphere nitrogen-fixing bacteria, and *FAMB126* in a live bacteria mass ratio of 4-5:1-2:1:2-3.

[0007] Furthermore, the phosphorus pentoxide content in calcium magnesium phosphate fertilizer is ≥70%.

[0008] Furthermore, based on the live bacteria ratio, the ratio of Lactobacillus reuteri strain LTR1318 to highly acid-resistant Acetobacter CM-067 is 1:0.2-0.5.

[0009] Furthermore, chitosan with a degree of deacetylation ≥ 85% was added to an acetic acid solution and stirred until completely dissolved to obtain a chitosan acetic acid solution; gallic acid was weighed and dissolved in anhydrous ethanol to prepare a gallic acid ethanol solution; the chitosan acetic acid solution was heated and then tapioca starch was added and stirred to activate it; the gallic acid ethanol solution was slowly added dropwise, and after the addition was completed, the reaction was carried out at a constant temperature to obtain modified chitosan.

[0010] Furthermore, take 100g of silkworm excrement, adjust the moisture content to 60-65%, and mix it evenly with 2-4g of modified chitosan to obtain a mixture; mix the Lactobacillus reuteri seed liquid with the highly acid-resistant Acetobacter seed liquid and stir evenly to obtain a composite bacterial solution; inoculate the composite bacterial solution into the mixture; then carry out fermentation treatment at a fermentation temperature of 60-65℃, and sterilize after fermentation for 1-2 days.

[0011] Furthermore, the fulvic acid content of the mineral-derived potassium fulvate is ≥50%, and the potassium oxide content is ≥12%.

[0012] Furthermore, the preparation method of eggshell powder after carbonization and steam treatment is as follows: prepare a vitamin C aqueous solution; put the eggshell powder into a muffle furnace, heat it to 380-420℃, and carbonize it at a constant temperature for 25-35 minutes; after carbonization, put the carbonized eggshell powder into a vitamin C aqueous solution with a solid-liquid ratio of 1:3-5, and then pass saturated steam through it, and treat it at a pressure of 0.1-0.15MPa and a temperature of 121-130℃ for 30-40 minutes.

[0013] Furthermore, the total viable count of the compound functional microbial agent is ≥2.0× CFU / g.

[0014] To better achieve the objectives of this invention, this invention also provides a method for preparing a soil re-acidification control and regulation material for grape cultivation, comprising the following steps: A. Preparation of rapid neutralization components; B. Preparation of slow-release fertilizer components; C. Preparation of activating and enhancing components; D. Preparation of microecological regulation components; E. Weigh out 20-35 parts of the rapid neutralizing component, 30-45 parts of the slow-release fertilizing component, 15-25 parts of the activating and enhancing component, and 5-15 parts of the microecological regulation component according to the following weight proportions, and mix them evenly to obtain the final product.

[0015] To better achieve the objectives of this invention, this invention also provides an application of a soil re-acidification control and regulation material for grape cultivation in the soil re-acidification control and remediation technology for grape cultivation.

[0016] Compared with the prior art, the beneficial effects of this invention are as follows: the soil improvement mechanism of this invention is a "physical-chemical-biological triple coupling" rather than a simple acid-base neutralization, thus achieving systematic improvement of acidic soils, as detailed below: (1) Chemical neutralization mechanism: Dolomite (CaMg(CO3)2) and limestone (CaCO3) react with soil H + A decomposition reaction occurs, releasing Ca. 2+ Mg 2+ Neutralizes acidity and simultaneously replaces Al adsorbed by soil colloids. 3+ Reduce aluminum toxicity; calcium magnesium phosphate fertilizer provides PO4. 3- , with Ca 2+ Mg 2+ It forms stable compounds, maintaining soil pH stability. The reaction equation is as follows: CaCO3+ 2H + = Ca 2+ + CO2↑ + H2O CaMg(CO3)2+ 4H + = Ca 2+ + + 2CO2↑ + 2H2O (2) Physical improvement mechanism: The fermentation products of silkworm excrement and the organic matter in the decomposed organic fertilizer work together with the mineral-derived potassium humate to promote the aggregation of soil particles through "cementation" to form a granular structure and improve soil porosity; coarse bone meal acts as a "skeleton" to further enhance the stability of soil structure, improve soil aeration and permeability, reduce soil bulk density, and avoid compaction.

[0017] (3) Bioactivation mechanism: After the microecological regulatory components colonize in the soil, they secrete cellulase, protease, phosphatase, etc., to decompose insoluble organic / inorganic nutrients and convert them into forms that can be absorbed by crops; the metabolic products of the microbial agent promote the reproduction of beneficial microorganisms (Bacillus, Actinomycetes) and inhibit harmful pathogens (Fusarium) in acidic soil; mineral-derived potassium humate provides carbon source for microorganisms, promotes the rapid proliferation of strains, and forms a virtuous cycle of "microorganism-fertilizer-soil".

[0018] (4) Component synergistic mechanism: fine-particle mineral raw materials rapidly reduce acidity, coarse-particle bone meal slowly releases fertilizer, organic materials improve structure, potassium humate activates nutrients, and microecological regulation components regulate the ecology. Each component forms a synergistic effect on the time scale (short-term-medium-long-term) and functional dimension (neutralization-fertilization-activation-regulation), achieving the improvement effect of "one agent with multiple effects and long-term stability". Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are merely some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without any creative effort.

[0020] Figure 1 This is a flowchart illustrating the preparation method of a soil re-acidification control and regulation material for grape cultivation according to the present invention. Detailed Implementation

[0021] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0022] Example 1: A method for preparing a soil re-acidification control and regulation material for grape cultivation, comprising the following steps: A. Preparation of the rapid neutralizing component. Weigh the following raw materials according to the specified parts by weight and mix them evenly: 10 parts of 80-mesh (fine particle size) dolomite powder, 8 parts of 80-mesh (fine particle size) limestone powder, and 2 parts of calcium magnesium phosphate fertilizer. The calcium magnesium phosphate fertilizer contains ≥70% phosphorus pentoxide; it is used to rapidly neutralize soil acidity and supplement... , P element helps alleviate aluminum toxicity.

[0023] B. Preparation of slow-release fertilizer components. Weigh the following raw materials according to the specified weight and mix them evenly: 10 parts of calcined bone meal (10 mesh, coarse particle size), 15 parts of silkworm excrement fermentation product, and 5 parts of decomposed organic fertilizer; used to slowly release nutrients such as phosphorus, calcium, and nitrogen, replenish soil organic matter, and improve soil structure.

[0024] The preparation method of silkworm excrement fermentation product is as follows: Take chitosan with a deacetylation degree ≥85%, add 1.5% (v / v) acetic acid solution at a solid-liquid ratio of 1:18 (g / mL), and stir magnetically at room temperature for 2 h until completely dissolved to obtain a chitosan acetic acid solution with a mass concentration of 3%; weigh gallic acid, with a chitosan to gallic acid mass ratio of 1:0.9, dissolve gallic acid in anhydrous ethanol to prepare a gallic acid ethanol solution with a mass concentration of 10%; heat the chitosan acetic acid solution to 55℃, add 1% cassava starch, and stir to activate for 15 min; slowly add gallic acid ethanol solution, and after the addition is complete, react at a constant temperature of 55℃ for 5 h to obtain modified chitosan. Take 100g of silkworm excrement, adjust the moisture content to 60%, and mix it evenly with 2g of modified chitosan to obtain a mixture. Mix the Lactobacillus reuteri seed liquid and the highly acid-resistant Acetobacter CM-067 seed liquid (the ratio of Lactobacillus reuteri strain LTR1318 to highly acid-resistant Acetobacter CM-067 is 1:0.2 according to the live bacteria ratio), stir evenly, and prepare a compound bacterial solution. Add the compound bacterial solution to the mixture at an inoculation rate of 10% (w / w). Then carry out fermentation treatment at 60℃, and sterilize after 1 day of fermentation. The silkworm excrement fermentation product is dried and sieved through a 50-mesh sieve. This method achieves the harmless treatment of silkworm excrement while maximizing the retention of its active components, which not only solves the problem of agricultural waste pollution, but also provides high-quality organic active nutrients for the conditioner.

[0025] C. Preparation of activating and enhancing components. Weigh the following raw materials by weight and mix them evenly: 8 parts of mineral-derived potassium fulvate and 7 parts of eggshell powder that has been carbonized and steam-treated; the fulvate content of the mineral-derived potassium fulvate is ≥50%, and the potassium oxide content is ≥12%; it is used to chelate heavy metal ions, activate mineral nutrients, promote microbial colonization, and enhance soil buffering capacity.

[0026] The method for preparing eggshell powder after carbonization and steam treatment is as follows: prepare a vitamin C aqueous solution with a mass fraction of 5%; put the eggshell powder into a muffle furnace and heat it to 380℃ at a rate of 5℃ / min, and carbonize it at a constant temperature for 25min; after carbonization, put the carbonized eggshell powder into a vitamin C aqueous solution with a solid-liquid ratio of 1:3 (g / mL), and then pass saturated steam through it and treat it at a pressure of 0.1MPa and a temperature of 121℃ for 30min.

[0027] D. Preparation of microecological regulation components—microbial functional agents. These agents are composed of *Priscilla megaterium* strain SY39 (accession number: CCTCC NO. M20251011), *Lactobacillus pentosaccharide* BNPV (accession number: CGMCC NO. 22174), *Nystatin NXT28* (accession number: CCTCC NO. M2017843), and *Rhizobium FAMB126* (accession number: CCTCC NO. M2018218) at a viable count / mass ratio of 4:1:1:2; the total viable count of the compound functional agent is ≥2.0 × 10⁻⁶. CFU / g; used to optimize soil microbial communities, inhibit pathogens, activate insoluble phosphorus and potassium, and regulate soil microecological balance.

[0028] E. Weigh out 20 parts of the rapid neutralizing component, 30 parts of the slow-release fertilizing component, 15 parts of the activation and enhancement component, and 5 parts of the microecological regulation component according to the following weight proportions, and mix them evenly to obtain the final product.

[0029] Example 2: A method for preparing a soil re-acidification control and regulation material for grape cultivation, comprising the following steps: A. Preparation of the rapid neutralizing component. Weigh the following raw materials according to the specified parts by weight and mix them evenly: 15 parts of 100-mesh (fine particle size) dolomite powder, 10 parts of 100-mesh (fine particle size) limestone powder, and 10 parts of calcium magnesium phosphate fertilizer. The calcium magnesium phosphate fertilizer contains ≥70% phosphorus pentoxide; it is used to rapidly neutralize soil acidity and supplement... , P element helps alleviate aluminum toxicity.

[0030] B. Preparation of slow-release fertilizer components. Weigh the following raw materials according to the specified weight and mix them evenly: 15 parts of calcined bone meal (30 mesh, coarse particle size), 20 parts of silkworm excrement fermentation product, and 10 parts of decomposed organic fertilizer; used to slowly release nutrients such as phosphorus, calcium, and nitrogen, replenish soil organic matter, and improve soil structure.

[0031] The preparation method of silkworm excrement fermentation product is as follows: Take chitosan with a deacetylation degree ≥85%, add 1.5% (v / v) acetic acid solution at a solid-liquid ratio of 1:18 (g / mL), and stir magnetically at room temperature for 2 h until completely dissolved to obtain a chitosan acetic acid solution with a mass concentration of 3%; weigh gallic acid, with a chitosan to gallic acid mass ratio of 1:0.9, dissolve gallic acid in anhydrous ethanol to prepare a gallic acid ethanol solution with a mass concentration of 10%; heat the chitosan acetic acid solution to 55℃, add 1% cassava starch, and stir to activate for 15 min; slowly add gallic acid ethanol solution, and after the addition is complete, react at a constant temperature of 55℃ for 5 h to obtain modified chitosan. Take 100g of silkworm excrement, adjust the moisture content to 65%, and mix it evenly with 4g of modified chitosan to obtain a mixture. Mix the Lactobacillus reuteri seed liquid and the highly acid-resistant Acetobacter CM-067 seed liquid (the ratio of Lactobacillus reuteri strain LTR1318 to highly acid-resistant Acetobacter CM-067 is 1:0.5 according to the live bacteria ratio), stir evenly, and prepare a compound bacterial solution. Add the compound bacterial solution to the mixture at an inoculation rate of 10% (w / w). Then carry out fermentation treatment at 65℃, and sterilize after 2 days of fermentation. The silkworm excrement fermentation product is dried and sieved through a 50-mesh sieve. This method achieves the harmless treatment of silkworm excrement while maximizing the retention of its active components, which not only solves the problem of agricultural waste pollution, but also provides high-quality organic active nutrients for the conditioner.

[0032] C. Preparation of activating and enhancing components. Weigh the following raw materials by weight and mix them evenly: 10 parts of mineral-derived potassium fulvate and 15 parts of eggshell powder that has been carbonized and steam-treated; the fulvic acid content of the mineral-derived potassium fulvate is ≥50%, and the potassium oxide content is ≥12%; it is used to chelate heavy metal ions, activate mineral nutrients, promote microbial colonization, and enhance soil buffering capacity.

[0033] The method for preparing eggshell powder after carbonization and steam treatment is as follows: prepare a vitamin C aqueous solution with a mass fraction of 5%; put the eggshell powder into a muffle furnace and heat it to 420℃ at a rate of 5℃ / min, and carbonize it at a constant temperature for 35min; after carbonization, put the carbonized eggshell powder into a vitamin C aqueous solution with a solid-liquid ratio of 1:5 (g / mL), and then pass saturated steam through it and treat it at a pressure of 0.15MPa and a temperature of 130℃ for 40min.

[0034] D. Preparation of microecological regulation components—microbial functional agents. These agents are composed of *Priscilla megaterium* strain SY39 (accession number: CCTCC NO. M20251011), *Lactobacillus pentosaccharide* BNPV (accession number: CGMCC NO. 22174), *Nystatin NXT28* (accession number: CCTCC NO. M2017843), and *Rhizobium FAMB126* (accession number: CCTCC NO. M2018218) at a viable count / mass ratio of 5:2:1:3; the total viable count of the compound functional agent is ≥2.0 × 10⁻⁶. CFU / g; used to optimize soil microbial communities, inhibit pathogens, activate insoluble phosphorus and potassium, and regulate soil microecological balance.

[0035] E. Weigh out 35 parts of the rapid neutralizing component, 45 parts of the slow-release fertilizing component, 25 parts of the activation and enhancement component, and 15 parts of the microecological regulation component according to the following weight proportions, and mix them evenly to obtain the final product.

[0036] Example 3: A method for preparing a soil re-acidification control and regulation material for grape cultivation, comprising the following steps: A. Preparation of the rapid neutralizing component. Weigh the following raw materials according to the specified parts by weight and mix them evenly: 12 parts of 90-mesh (fine particle size) dolomite powder, 9 parts of 90-mesh (fine particle size) limestone powder, and 5 parts of calcium magnesium phosphate fertilizer. The calcium magnesium phosphate fertilizer contains ≥70% phosphorus pentoxide; it is used to rapidly neutralize soil acidity and supplement... , P element helps alleviate aluminum toxicity.

[0037] B. Preparation of slow-release fertilizer components. Weigh the following raw materials according to the specified weight and mix them evenly: 12 parts of calcined bone meal (20 mesh, coarse particle size), 18 parts of silkworm excrement fermentation product, and 7 parts of decomposed organic fertilizer; used to slowly release nutrients such as phosphorus, calcium, and nitrogen, replenish soil organic matter, and improve soil structure.

[0038] The preparation method of silkworm excrement fermentation product is as follows: Take chitosan with a deacetylation degree ≥85%, add 1.5% (v / v) acetic acid solution at a solid-liquid ratio of 1:18 (g / mL), and stir magnetically at room temperature for 2 h until completely dissolved to obtain a chitosan acetic acid solution with a mass concentration of 3%; weigh gallic acid, with a chitosan to gallic acid mass ratio of 1:0.9, dissolve gallic acid in anhydrous ethanol to prepare a gallic acid ethanol solution with a mass concentration of 10%; heat the chitosan acetic acid solution to 55℃, add 1% cassava starch, and stir to activate for 15 min; slowly add gallic acid ethanol solution, and after the addition is complete, react at a constant temperature of 55℃ for 5 h to obtain modified chitosan. Take 100g of silkworm excrement, adjust the moisture content to 62%, and mix it evenly with 3g of modified chitosan to obtain a mixture. Mix the Lactobacillus reuteri seed liquid and the highly acid-resistant Acetobacter CM-067 seed liquid (the ratio of Lactobacillus reuteri strain LTR1318 to highly acid-resistant Acetobacter CM-067 is 1:0.3 according to the live bacteria ratio), stir evenly, and prepare a compound bacterial solution. Add the compound bacterial solution to the mixture at an inoculation rate of 10% (w / w). Then carry out fermentation treatment at a fermentation temperature of 62℃, and sterilize after 1 day of fermentation. The silkworm excrement fermentation product is dried and sieved through a 50-mesh sieve. This method achieves the harmless treatment of silkworm excrement while maximizing the retention of its active components, which not only solves the problem of agricultural waste pollution, but also provides high-quality organic active nutrients for conditioning agents.

[0039] C. Preparation of activating and enhancing components. Weigh the following raw materials by weight and mix them evenly: 9 parts of mineral-derived potassium fulvate and 11 parts of eggshell powder that has been carbonized and steam-treated; the fulvic acid content of the mineral-derived potassium fulvate is ≥50%, and the potassium oxide content is ≥12%; it is used to chelate heavy metal ions, activate mineral nutrients, promote microbial colonization, and enhance soil buffering capacity.

[0040] The method for preparing eggshell powder after carbonization and steam treatment is as follows: prepare a vitamin C aqueous solution with a mass fraction of 5%; put the eggshell powder into a muffle furnace and heat it to 385℃ at a rate of 5℃ / min, and carbonize it at a constant temperature for 28min; after carbonization, put the carbonized eggshell powder into a vitamin C aqueous solution with a solid-liquid ratio of 1:4 (g / mL), and then pass saturated steam through it and treat it at a pressure of 0.12MPa and a temperature of 125℃ for 32min.

[0041] D. Preparation of microecological regulation components—microbial functional agents. These agents are composed of *Priscilla megaterium* strain SY39 (accession number: CCTCC NO. M20251011), *Lactobacillus pentosaccharide* BNPV (accession number: CGMCC NO. 22174), *Nystatin NXT28* (accession number: CCTCC NO. M2017843), and *Rhizobium FAMB126* (accession number: CCTCC NO. M2018218) at a viable count / mass ratio of 4:2:1:3; the total viable count of the compound functional agent is ≥2.0 × 10⁻⁶. CFU / g; used to optimize soil microbial communities, inhibit pathogens, activate insoluble phosphorus and potassium, and regulate soil microecological balance.

[0042] E. Weigh out 25 parts of the rapid neutralizing component, 35 parts of the slow-release fertilizing component, 18 parts of the activation and enhancement component, and 6 parts of the microecological regulation component according to the following weight proportions, and mix them evenly to obtain the final product.

[0043] Comparative Example 1: Unlike Example 3, the preparation method of the silkworm excrement fermentation product is as follows: Take 100g of silkworm excrement, adjust the moisture content to 62%, and mix it evenly with 3g of chitosan to obtain a mixture; mix the Lactobacillus reuteri seed liquid and the highly acid-resistant Acetobacter seed liquid (according to the live bacteria ratio, the ratio of Lactobacillus reuteri strain LTR1318 to highly acid-resistant Acetobacter CM-067 is 1:0.3), stir evenly, and obtain a composite bacterial solution; add the composite bacterial solution to the mixture at an inoculation rate of 10% (w / w); then carry out fermentation treatment at a fermentation temperature of 62℃, and sterilize after 1 day of fermentation; the silkworm excrement fermentation product is dried and sieved through a 50-mesh sieve.

[0044] Comparative Example 2: Unlike Example 3, 11 parts of the carbonized and steam-treated eggshell powder in the activation and enhancement component were replaced with eggshell powder dried at 60 degrees Celsius.

[0045] Comparative Example 3: Unlike Example 3, the microbial functional agent was composed of *Priscilla megaterium* strain SY39 (accession number: CCTCC NO. M20251011), *Lactobacillus pentosaccharide* BNPV (accession number: CGMCC NO. 22174), *Nystatin NXT28* (accession number: CCTCC NO. M2017843), and *Rhizobium FAMB126* (accession number: CCTCC NO. M2018218) in a viable count mass ratio of 3:2.5:1:3.5; the total viable count of the composite functional agent was ≥2.0 × 10⁻⁶. CFU / g.

[0046] Experimental Example: Verification Experiment on the Prevention and Remediation Effects of Re-acidification in Acidic Red Soil for Grape Cultivation The tested soil was a typical acidic red soil from southern China, with the following basic physicochemical properties: pH 4.2, organic matter content 12.6 g / kg, available phosphorus content 8.3 mg / kg, available potassium content 52.1 mg / kg, and bulk density 1.58 g / cm³. 3 The aluminum ion content was 125.4 mg / kg, and the total number of beneficial microorganisms (actinomycetes and Bacillus) was 2.3 × 10⁻⁶. 6 CFU / g, soil-borne disease (wilt) incidence rate 28%.

[0047] Test materials: Four soil samples were selected and applied to the soil re-acidification control and regulation material for grape cultivation prepared in Example 3, and the remediation materials prepared in Comparative Example 1, Comparative Example 2, and Comparative Example 3, respectively, at a rate of 2% (w / w).

[0048] Cultivation and Management: Transplant one-year-old grape seedlings (variety: Kyoho) with uniform growth and place them in a greenhouse for 180 days without applying any other fertilizers.

[0049] Detection indicators: (1) Soil physicochemical indicators: Determine soil organic matter, bulk density, available phosphorus, available potassium, aluminum ion content, pH stability period, and soil buffering capacity. (2) Microbiological indicators: Determine the number of beneficial microorganisms in the soil, and detect the activity retention rate and soil colonization rate of functional strains in the remediation materials. (3) Diseases: Track and record the incidence of grape wilt disease.

[0050] The changes in the calculated indicators are shown in Table 1.

[0051] Table 1

[0052] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A material for controlling and regulating soil re-acidification in grape cultivation, characterized in that, The raw materials include the following parts by weight: 20-35 parts of rapid neutralizing component, 30-45 parts of slow-release fertilizing component, 15-25 parts of activating and enhancing component, and 5-15 parts of microecological regulation component; The following raw materials are used for rapid neutralization: 10-15 parts of 80-100 mesh dolomite powder, 8-10 parts of 80-100 mesh limestone powder, and 2-10 parts of calcium magnesium phosphate fertilizer. The slow-release fertilizer component consists of the following raw materials in parts by weight: 10-15 parts of calcined bone meal (10-30 mesh), 15-20 parts of silkworm excrement fermentation product, and 5-10 parts of well-rotted organic fertilizer; wherein, the preparation method of the silkworm excrement fermentation product is as follows: first, chitosan is grafted with gallic acid to obtain modified chitosan, and then silkworm excrement, modified chitosan, Lactobacillus reuteri strain LTR1318, and highly acid-resistant Acetobacter CM-067 are mixed, fermented, and then sterilized; The activating and enhancing components are made from the following raw materials in parts by weight: 8-10 parts of mineral-derived potassium humate, and 7-15 parts of eggshell powder that has been carbonized and steam-treated. The microecological regulation component is a microbial functional agent, which is a compound of *Priscilla megaterium* strain SY39, *Lactobacillus pentosus* BNPV, *NXT28* rhizosphere nitrogen-fixing bacteria, and *FAMB126* in a live bacteria mass ratio of 4-5:1-2:1:2-3.

2. The soil re-acidification control and regulation material for grape cultivation according to claim 1, characterized in that, The phosphorus pentoxide content in calcium magnesium phosphate fertilizer is ≥70%.

3. The soil re-acidification control and regulation material for grape cultivation according to claim 1, characterized in that, Based on the live bacteria ratio, the dosage ratio of Lactobacillus reuteri strain LTR1318 to highly acid-resistant Acetobacter CM-067 is 1:0.2-0.

5.

4. The soil re-acidification control and regulation material for grape cultivation according to claim 1, characterized in that, Chitosan with a degree of deacetylation ≥ 85% was added to an acetic acid solution and stirred until completely dissolved to obtain a chitosan acetic acid solution. Gallic acid was weighed and dissolved in anhydrous ethanol to prepare a gallic acid ethanol solution. The chitosan acetic acid solution was heated and then tapioca starch was added and stirred to activate it. The gallic acid ethanol solution was slowly added dropwise, and after the addition was complete, the reaction was carried out at a constant temperature to obtain modified chitosan.

5. The soil re-acidification control and regulation material for grape cultivation according to claim 1, characterized in that, Take 100g of silkworm excrement, adjust the moisture content to 60-65%, and mix it evenly with 2-4g of modified chitosan to obtain a mixture. Mix the Lactobacillus reuteri seed liquid with the highly acid-resistant Acetobacter seed liquid and stir evenly to obtain a compound bacterial solution. Inoculate the compound bacterial solution into the mixture. Then carry out fermentation treatment at a temperature of 60-65℃. After fermentation for 1-2 days, sterilize.

6. The soil re-acidification control and regulation material for grape cultivation according to claim 1, characterized in that, The fulvic acid content of the mineral-derived potassium fulvate is ≥50%, and the potassium oxide content is ≥12%.

7. The soil re-acidification control and regulation material for grape cultivation according to claim 1, characterized in that, The method for preparing eggshell powder after carbonization and steam treatment is as follows: Prepare a vitamin C aqueous solution; put the eggshell powder into a muffle furnace, heat it to 380-420℃, and carbonize it at a constant temperature for 25-35 minutes; after carbonization, put the carbonized eggshell powder into a vitamin C aqueous solution with a solid-liquid ratio of 1:3-5, and then pass saturated steam through it, and treat it at a pressure of 0.1-0.15MPa and a temperature of 121-130℃ for 30-40 minutes.

8. The soil re-acidification control and regulation material for grape cultivation according to claim 1, characterized in that, The total viable count of the compound functional microbial agent is ≥2.0× CFU / g.

9. The method for preparing the soil re-acidification control and regulation material for grape cultivation according to any one of claims 1-8, characterized in that, Includes the following steps: A. Preparation of rapid neutralization components; B. Preparation of slow-release fertilizer components; C. Preparation of activating and enhancing components; D. Preparation of microecological regulation components; E. Weigh out 20-35 parts of the rapid neutralizing component, 30-45 parts of the slow-release fertilizing component, 15-25 parts of the activating and enhancing component, and 5-15 parts of the microecological regulation component according to the following weight proportions, and mix them evenly to obtain the final product.

10. The application of a soil re-acidification control and regulation material for grape cultivation according to any one of claims 1-8 in the soil re-acidification control and remediation technology for grape cultivation.

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