Anti-hair loss shampoo containing active ingredients of chlorella vulgaris and preparation method thereof

Abstract

The present application relates to a kind of chlorella active ingredient-containing anti-hair loss shampoo and its preparation method.The chlorella active ingredient-containing anti-hair loss shampoo includes chlorella active ingredient, anti-hair loss active composition, surfactant, scalp barrier repair composition and deionized water;The scalp barrier repair composition includes the combination of ectoine, ceramide and sodium hyaluronate.The anti-hair loss shampoo of the present application is complementary with each component, and realizes the cleaning regulation, system stability, reduces irritation, activates follicle, improves microcirculation and other multiple ways synergistic effect of anti-hair loss and cleaning effect, the target point of the active ingredient of the product is clear, suitable for androgenic alopecia, alopecia areata, telogen effluvium and other various types of hair loss, in addition, the product of the present application is light in texture, not greasy, and there is no irritation after use.

Description

Technical Field

[0001] This invention belongs to the field of biotechnology, specifically relating to an anti-hair loss shampoo containing Chlorella active ingredients and its preparation method. Background Technology

[0002] With increasing life pressures, worsening environmental pollution, and changing lifestyles in modern society, hair loss has become a common scalp health concern, leading to a growing market demand for safe and effective anti-hair loss shampoos and conditioners. Existing anti-hair loss shampoos largely rely on chemically synthesized ingredients to build their efficacy. Mainstream formulas often use sulfate surfactants as the core cleansing agent, combined with chemically active ingredients such as minoxidil, finasteride, zinc pyrithione (ZPT), and high-concentration salicylic acid to achieve their anti-hair loss claims.

[0003] CN118078847A discloses an anti-hair loss composition, its preparation method, and its application. The composition contains minoxidil. In a mouse model of androgenetic alopecia, this composition has been shown to effectively improve testosterone-induced hair and follicle reduction, with significantly better results than single components or combinations of minoxidil with other forms. Efficacy evaluation tests show that topical minoxidil and compounds of formula I can significantly improve the efficacy and overall effectiveness of minoxidil. Furthermore, the composition has no adverse skin reactions and high safety.

[0004] CN115957292A discloses a compound spray for preventing hair loss containing finasteride, belonging to the field of biomedical technology. The raw materials of this compound spray, by weight, include: 0.01-0.08 parts finasteride, 0.01-1 parts chitosan, 1.2-3 parts vitamin B, 1-2 parts saponins, 0.8-1 parts alkaloids, 0.08-0.12 parts fruit acids, 12-20 parts camellia, 15-22 parts aloe vera, 10-15 parts ginger, 8-15 parts eclipta prostrata, and 8-12 parts camphor. This invention addresses the problem that finasteride is difficult to penetrate the skin due to its lipophilic and hydrophilic nature. Finasteride is encapsulated in chitosan, and through the synergistic effect of the various raw materials, it effectively prevents and treats seborrheic alopecia by improving the scalp environment, promoting scalp cell regeneration, promoting hair growth, and inhibiting 5α-reductase activity. The treatment effect is good and recurrence is not common.

[0005] However, while the aforementioned products can quickly achieve short-term effects such as cleansing, oil control, or inhibiting hair follicle atrophy, they generally suffer from strong irritation. Long-term use can easily damage the scalp barrier, causing discomfort such as itching, redness, dryness, and sensitivity, and may even exacerbate the imbalance of scalp sebum secretion, creating a vicious cycle of "preventing hair loss but causing more hair loss instead."

[0006] Meanwhile, chemically synthesized formulations suffer from limited efficacy and poor compatibility. Most products focus solely on oil control or simple antibacterial effects, lacking a systematic approach to regulating hair follicle microcirculation and scalp flora balance, making it difficult to address the root causes of common hair loss types such as androgenetic alopecia and postpartum hair loss. Furthermore, some chemical components (such as ZPT) pose environmental toxicity or potential health risks and are restricted by the EU, failing to meet current consumer demands for gentle, natural, and safe hair care products. Therefore, developing a product primarily composed of natural ingredients, low in irritation, balancing cleansing power with anti-hair loss efficacy, and capable of repairing the scalp barrier has become a crucial issue urgently needing to be addressed in the current hair loss shampoo and conditioner technology field. Summary of the Invention

[0007] To address the shortcomings of existing technologies, the present invention aims to provide an anti-hair loss shampoo containing Chlorella active ingredients and its preparation method.

[0008] To achieve this objective, the present invention adopts the following technical solution: In a first aspect, the present invention provides an anti-hair loss shampoo containing Chlorella active ingredients, wherein the components of the anti-hair loss shampoo containing Chlorella active ingredients include Chlorella active ingredients, an anti-hair loss active composition, a surfactant, a scalp barrier repair composition, and deionized water; The scalp barrier repair composition comprises a combination of ectoine, ceramide, and sodium hyaluronate.

[0009] The anti-hair loss shampoo of this invention utilizes the complementary properties of Chlorella active ingredients, anti-hair loss active composition, surfactant, and scalp barrier repair composition to achieve a synergistic effect of anti-hair loss and hair growth, as well as cleansing and regulating, system stabilization, irritation reduction, hair follicle activation, and microcirculation improvement. The active ingredients of the product have clear targets and are suitable for various types of hair loss, such as androgenetic alopecia, alopecia areata, and telogen effluvium. In addition, the product of this invention has a refreshing and non-greasy texture and does not cause scalp irritation after use.

[0010] Preferably, by weight, the anti-hair loss shampoo containing Chlorella active ingredients comprises 0.1-5 parts of Chlorella active ingredients, 0.1-6 parts of anti-hair loss active composition, 5-25 parts of surfactant, 0.05-2 parts of scalp barrier repair composition, and 60-95 parts of deionized water.

[0011] The amount of Chlorella active ingredient in the anti-hair loss shampoo of the present invention can be 0.5 parts, 0.8 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts or 4.5 parts, etc.; The amount of the anti-hair loss active composition can be 0.3 parts, 0.5 parts, 0.8 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts or 5.5 parts, etc.; The amount of the surfactant can be 8 parts, 10 parts, 12 parts, 14 parts, 15 parts, 16 parts, 20 parts, 22 parts or 24 parts, etc.; The dosage of the scalp barrier repair composition can be 0.08 parts, 0.1 parts, 0.3 parts, 0.5 parts, 0.8 parts, 1 part, 1.1 parts, 1.3 parts, 1.5 parts, 1.7 parts, or 1.9 parts, etc. The amount of deionized water used can be 60 parts, 65 parts, 70 parts, 75 parts, 80 parts, 85 parts, or 90 parts, etc.

[0012] The active ingredients of Chlorella described in this invention are obtained from Chlorella through specific cell wall disruption, separation, and purification processes. A preferred preparation method includes the following steps: (1) Take Chlorella mud or Chlorella concentrate and adjust the pH of the system to 8-10; (2) Treat at 70-80℃ for 20-60 min to promote cell wall disruption and release of active substances; (3) Gradient centrifugation was used to remove cell debris and impurities; (4) Further separation, purification and stabilization of the supernatant to obtain Chlorella active components; (5) The obtained active components are used after passing quality inspection.

[0013] Preferably, the obtained Chlorella active ingredients should have good stability and biocompatibility.

[0014] The anti-hair loss shampoo of this invention contains Chlorella active ingredients, whose Chlorella growth factor (CGF) contains nucleotides and nucleosides, which can stimulate the proliferation and differentiation of dermal papilla cells of hair follicles, prolong the anagen phase of hair follicles, awaken the telogen phase of hair follicles to re-enter the anagen phase, and inhibit 5α-reductase activity, reducing the damage of DHT to hair follicles. It is further supplemented with multiple surfactants and conditioning agents to complement each other, achieving a synergistic effect of anti-hair loss and hair growth, as well as cleansing, regulating, stabilizing the system, reducing irritation, activating hair follicles, and improving microcirculation.

[0015] Preferably, the surfactant comprises any one or a combination of at least two of sodium lauryl ether sulfate, cocamidopropyl betaine, sodium lauroyl sarcosinate, or sodium lauroyl methyl taurate.

[0016] Preferably, the surfactant includes sodium lauryl ether sulfate, cocamidopropyl betaine, sodium lauroyl sarcosinate, and sodium lauroyl methyl taurate.

[0017] Preferably, the mass ratio of sodium lauryl ether sulfate, cocamidopropyl betaine, sodium lauroyl sarcosinate and sodium lauroyl methyl taurate is (5-10):(5-7):(2-4):(1-3).

[0018] "5-10" can be 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, or 9.5, etc. “5-7” can be 5.1, 5.3, 5.5, 5.8, 6, 6.2, 6.5, 6.7 or 6.9, etc.; "2-4" can be 2.1, 2.3, 2.5, 2.8, 3, 3.2, 3.5, 3.7, or 3.9, etc.; “1-3” can be 1.2, 1.4, 1.5, 1.6, 1.8, 2.0, 2.2, 2.4, 2.5, 2.6, 2.8 or 2.9, etc.

[0019] Preferably, the anti-hair loss active composition comprises a combination of at least two of caffeine, nicotinamide, panthenol, arborvitae leaf extract, adenosine, acetyl tetrapeptide-3, or biotin.

[0020] Preferably, the anti-hair loss active composition is a combination of caffeine, nicotinamide, panthenol, adenosine, acetyl tetrapeptide-3 and arborvitae leaf extract.

[0021] Preferably, the mass ratio of caffeine, nicotinamide, panthenol, adenosine, acetyl tetrapeptide-3 and arborvitae leaf extract is (0.1-1):(0.5-2):(0.1-1):(0.05-0.5):(0.01-0.2):(0.1-1).

[0022] The first "0.1-1" can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, or 0.9, etc.; "0.5-2" can be 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, or 1.9, etc. The second "0.1-1" can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, or 0.9, etc.; "0.05-0.5" can be 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, or 0.4, etc. "0.01-0.2" can be 0.03, 0.05, 0.08, 0.1, 0.12, 0.15, 0.17, or 0.19, etc. The third "0.1-1" can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, or 0.9, etc.

[0023] Preferably, the mass ratio of ectoine, ceramide and sodium hyaluronate is (1-3):(0.1-1):(0.5-2).

[0024] The values ​​“1-3” can be 1.1, 1.3, 1.5, 1.7, 1.9, 2.1, 2.2, 2.4, 2.6, 2.7, 2.8, or 2.9, etc. "0.1-1" can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, or 0.9, etc.; "0.5-2" includes 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, or 1.9, etc.

[0025] Preferably, the anti-hair loss shampoo also includes excipients.

[0026] Preferably, the excipients include any one or a combination of at least two of the following: pH adjuster, chelating agent, preservative, thickener, or fragrance.

[0027] Preferably, the excipients include any one or a combination of at least two of glycerin, citric acid, sodium chloride, sodium benzoate, or fragrance.

[0028] In a second aspect, the present invention provides a method for preparing an anti-hair loss shampoo containing Chlorella active ingredients as described in the first aspect, the method comprising mixing Chlorella active ingredients, an anti-hair loss active composition, a surfactant and a scalp barrier repair composition uniformly in deionized water to obtain the anti-hair loss shampoo containing Chlorella active ingredients.

[0029] Preferably, the preparation method includes: (1) Heat deionized water and add the anti-hair loss active composition, then mix well; (2) Then add the surfactants one by one and mix well; (3) After cooling, add Chlorella active ingredients and scalp barrier repair composition, mix evenly, add citric acid to adjust pH, then add sodium benzoate and fragrance, mix evenly to obtain the anti-hair loss shampoo containing Chlorella active ingredients.

[0030] Preferably, the heating in step (1) to 70-75°C can be, for example, 70.5°C, 71°C, 71.5°C, 72°C, 72.5°C, 73°C, 73.5°C, 74°C, 74.2°C, or 74.5°C.

[0031] Preferably, the temperature drop to 40-45℃ in step (3) can be, for example, 40.5℃, 41℃, 41.5℃, 42℃, 42.5℃, 43℃, 43.5℃, 44℃, 44.2℃ or 44.5℃.

[0032] Preferably, the pH adjustment to 5.5-6.5 in step (3) can be, for example, 5.6, 5.7, 5.8, 5.9, 6.0, 6.1, 6.2, 6.3, 6.35 or 6.4.

[0033] All the specific point values ​​within the above range can be selected, and will not be elaborated on here.

[0034] Compared with the prior art, the present invention has the following beneficial effects: (1) By introducing Chlorella active ingredients, this invention can improve the scalp microenvironment, enhance the scalp barrier function, and provide a good growth environment for hair follicles.

[0035] (2) The product of the present invention can improve hair loss problems through multiple pathways by the synergistic effect of classic anti-hair loss active ingredients such as caffeine, nicotinamide, panthenol, adenosine, acetyl tetrapeptide-3 and arborvitae leaf extract.

[0036] (3) It adopts a mild surfactant system, which does not contain diethanolamine or sodium lauryl sulfate, thus reducing the risk of irritation and improving the safety of long-term use.

[0037] (4) By introducing repairing ingredients such as ectoine, ceramide and sodium hyaluronate, this invention can improve problems such as dry and sensitive scalp and enhance the user experience.

[0038] (5) This invention constructs a multi-target synergistic anti-hair loss mechanism of “scalp microenvironment improvement + classic anti-hair loss activity promotion + barrier repair”, which has good prospects for industrial application. Detailed Implementation

[0039] To further illustrate the technical means and effects of the present invention, the following describes the technical solution of the present invention in conjunction with preferred embodiments of the present invention. However, the present invention is not limited to the scope of the embodiments.

[0040] The following detailed description of the features and advantages of the present invention is sufficient to enable those skilled in the art to understand the technical content of the present invention and implement it accordingly. Furthermore, based on this specification and the claims, those skilled in the art can easily understand the related objectives and advantages of the present invention.

[0041] The terminology and expressions used herein are for descriptive purposes only, and the invention should not be limited to these terms and expressions. The use of these terms and expressions does not imply the exclusion of any illustrative and descriptive equivalents (or parts thereof), and it should be recognized that various modifications that may exist should also be included within the scope of the claims. Other modifications, variations, and substitutions may also exist. Accordingly, the claims should be considered to cover all such equivalents.

[0042] The technical solution of the present invention will be further illustrated below through specific embodiments. Those skilled in the art should understand that the embodiments described are merely illustrative of the present invention and should not be construed as limiting the invention in any way.

[0043] The weight parts mentioned in the following examples and comparative examples are based on the actual active ingredients contained in commercially available raw materials. These commercially available raw materials may also selectively contain any one or a combination of at least two of the following: solvents, fillers, diluents, stabilizers, pH adjusters, antibacterial agents, antioxidants, or impurities within permissible limits.

[0044] The sources of some of the raw materials used in the embodiments and comparative examples of this invention are shown in Table 1 below: Table 1 All other raw materials can be used as long as they are purchased from authorized distributors.

[0045] Examples 1-3 The embodiments provided three types of anti-hair loss shampoos containing Chlorella active ingredients. The components and dosages of the anti-hair loss shampoos are shown in Table 2.

[0046] Table 2 The preparation method of the anti-hair loss shampoo in Example 1 is as follows: (1) Heat deionized water to 72°C and add the anti-hair loss active composition, then mix well; (2) Then add the surfactants one by one and mix well; (3) After cooling to 42.5℃, add Chlorella active ingredients and scalp barrier repair composition, mix evenly, add citric acid to adjust pH to 6.0, then add sodium benzoate and fragrance, mix evenly to obtain the anti-hair loss shampoo containing Chlorella active ingredients.

[0047] The preparation methods of the anti-hair loss shampoos in Examples 2 and 3 are the same as those in Example 1.

[0048] Example 4 This embodiment provides an anti-hair loss shampoo containing Chlorella active ingredients. The only difference between this shampoo and Example 1 is that it does not contain caffeine. The reduced amount is made up by nicotinamide, panthenol, adenosine, acetyl tetrapeptide-3 and Platycladus orientalis leaf extract in the same proportions. The remaining components and amounts are the same as in Example 1. The preparation method is the same as in Example 1.

[0049] Example 5 This embodiment provides an anti-hair loss shampoo containing Chlorella active ingredients. The only difference between this shampoo and that of Example 1 is that it does not include niacinamide in its components. The reduced amount is made up by caffeine, panthenol, adenosine, acetyl tetrapeptide-3, and Platycladus orientalis leaf extract in the specified proportions. The remaining components and their amounts are the same as in Example 1, and the preparation method is the same as in Example 1.

[0050] Example 6 This embodiment provides an anti-hair loss shampoo containing Chlorella active ingredients. The only difference between this shampoo and that of Example 1 is that it does not include panthenol in its components. The reduced amount is made up by caffeine, nicotinamide, adenosine, acetyl tetrapeptide-3, and Platycladus orientalis leaf extract in the specified proportions. The remaining components and their amounts are the same as in Example 1, and the preparation method is the same as in Example 1.

[0051] Example 7 This embodiment provides an anti-hair loss shampoo containing Chlorella active ingredients. The only difference between this shampoo and Example 1 is that it does not include adenosine in its components. The reduced amount is made up by caffeine, nicotinamide, panthenol, acetyl tetrapeptide-3, and Platycladus orientalis leaf extract in the specified proportions. The remaining components and their amounts are the same as in Example 1, and the preparation method is the same as in Example 1.

[0052] Example 8 This embodiment provides an anti-hair loss shampoo containing Chlorella active ingredients. The only difference between this shampoo and that of Example 1 is that it does not include acetyl tetrapeptide-3 in the composition. The reduced amount is made up by caffeine, nicotinamide, panthenol, adenosine, and arborvitae leaf extract in the specified proportions. The remaining components and amounts are the same as in Example 1, and the preparation method is the same as in Example 1.

[0053] Example 9 This embodiment provides an anti-hair loss shampoo containing Chlorella active ingredients. The only difference between this shampoo and Example 1 is that it does not include Platycladus orientalis leaf extract in its composition. The reduced amount is made up by caffeine, nicotinamide, panthenol, adenosine, and acetyl tetrapeptide-3 in the same dosage ratio. The remaining components and their amounts are the same as in Example 1, and the preparation method is the same as in Example 1.

[0054] Example 10 This embodiment provides an anti-hair loss shampoo containing Chlorella active ingredients. The only difference between this shampoo and Example 1 is that the surfactant does not include sodium lauryl ether sulfate. The reduction in sodium lauryl ether sulfate is made up by cocamidopropyl betaine, sodium lauroyl sarcosinate, and sodium lauroyl methyl taurate in the specified dosage ratio. The remaining components and their amounts are the same as in Example 1, and the preparation method is the same as in Example 1.

[0055] Example 11 This embodiment provides an anti-hair loss shampoo containing Chlorella active ingredients. The only difference between this shampoo and Example 1 is that the surfactant does not include cocamidopropyl betaine. The reduced amount is made up by sodium lauryl ether sulfate, sodium lauroyl sarcosinate, and sodium lauroyl methyl taurate in the specified proportions. The remaining components and amounts are the same as in Example 1, and the preparation method is the same as in Example 1.

[0056] Example 12 This embodiment provides an anti-hair loss shampoo containing Chlorella active ingredients. The only difference between this shampoo and Example 1 is that the surfactant does not include sodium lauroyl sarcosinate. The reduction in sodium laureth sulfate, cocamidopropyl betaine, and sodium lauroyl methyl taurate are made up by the proportions of sodium lauryl ether sulfate, cocamidopropyl betaine, and sodium lauroyl methyl taurate. The remaining components and amounts are the same as in Example 1, and the preparation method is the same as in Example 1.

[0057] Example 13 This embodiment provides an anti-hair loss shampoo containing Chlorella active ingredients. The only difference between this shampoo and Example 1 is that the surfactant does not include sodium lauroyl methyl taurate. The reduction in sodium laureth sulfate, cocamidopropyl betaine, and sodium lauroyl sarcosinate are made up by the proportion of sodium laureth sulfate, cocamidopropyl betaine, and sodium lauroyl sarcosinate. The remaining components and their amounts are the same as in Example 1, and the preparation method is the same as in Example 1.

[0058] Comparative Example 1 This comparative example provides an anti-hair loss shampoo containing Chlorella active ingredients. The only difference between this shampoo and Example 1 is that it does not contain Chlorella active ingredients. The reduction is made up by deionized water. The remaining components and amounts are the same as in Example 1. The preparation method is the same as in Example 1.

[0059] Comparative Example 2 This comparative example provides an anti-hair loss shampoo containing Chlorella active ingredients. The only difference between this shampoo and Example 1 is that the anti-hair loss active composition is not included in the composition. The reduction is made up by the components of the scalp barrier repair composition according to the dosage ratio. The remaining components and their addition amounts are the same as in Example 1. The preparation method is the same as in Example 1.

[0060] Comparative Example 3 This comparative example provides an anti-hair loss shampoo containing Chlorella active ingredients. The only difference between this shampoo and Example 1 is that the scalp barrier repair composition is not included in the composition. The reduction is made up by the proportion of each component of the anti-hair loss active composition. The remaining components and their amounts are the same as in Example 1. The preparation method is the same as in Example 1.

[0061] Comparative Example 4 This comparative example provides an anti-hair loss shampoo containing Chlorella active ingredients. The only difference between this shampoo and Example 1 is that the scalp barrier repair composition does not include ectoine. The reduction is made up by ceramide and sodium hyaluronate in the dosage ratio. The remaining components and their addition amounts are the same as in Example 1. The preparation method is the same as in Example 1.

[0062] Comparative Example 5 This comparative example provides an anti-hair loss shampoo containing Chlorella active ingredients. The only difference between this shampoo and Example 1 is that the scalp barrier repair composition does not include ceramides. The reduction in ceramides is made up by ectoine and sodium hyaluronate in the specified dosage ratio. The remaining components and their amounts are the same as in Example 1, and the preparation method is the same as in Example 1.

[0063] Comparative Example 6 This comparative example provides an anti-hair loss shampoo containing Chlorella active ingredients. The only difference between this shampoo and Example 1 is that the scalp barrier repair composition does not include sodium hyaluronate. The reduction is made up by ectoine and ceramide in the dosage ratio. The remaining components and their addition amounts are the same as in Example 1. The preparation method is the same as in Example 1.

[0064] Test Example 1: 5α-Reductase Activity Inhibition Rate Test 5α-reductase in the scalp can catalyze the conversion of testosterone into dihydrotestosterone (DHT). DHT continuously attacks hair follicles, leading to follicle miniaturization, decreased activity of dermal papilla cells, shortened follicle growth phase, and premature telogen phase, ultimately causing hair to become thinner, softer, and fall out. This product possesses an excellent 5α-reductase inhibition rate, which is its core technological advantage in achieving root-cause hair loss prevention.

[0065] Test samples: Samples from Examples 1-13 and Comparative Examples 1-6; Test method: Specifically includes the following steps: (1) Preparation of crude 5α-reducing enzyme: Male SD rats were euthanized by intraperitoneal overdose of 10% chloral hydrate. The liver tissue was quickly dissected and removed. Excess blood was washed away with physiological saline. Phosphate buffer (pH=7.4) was added at a mass ratio of 1:5. Magnetic beads were added and homogenized in a homogenizer. The homogenate was centrifuged at 10000 r / min for 10 min at 4℃ in a refrigerated centrifuge. The supernatant was collected and centrifuged once more. The supernatant was the crude enzyme solution and stored at -20℃ for later use.

[0066] (2) Protein content determination: The protein concentration in the crude enzyme solution was determined using a protein quantification kit. Following the kit's instructions, protein standards were diluted to concentrations of 0, 10, 20, 40, 80, 120, 160, and 200 μg / mL to create a series of standard protein solutions. 200 μL of G250 staining solution was added to each of these standard solutions, and after mixing and incubation at room temperature for 3-5 min, the absorbance was measured at 595 nm using a microplate reader. A standard curve was plotted with protein absorbance (Y) on the ordinate and protein concentration (X, mg / mL) on the abscissa. The crude enzyme protein concentration was calculated using the protein standard curve equation.

[0067] (3) Determination of 5α-reductase activity inhibition The total reaction volume of the 5α-reductase-catalyzed reaction was 200 μL. 120 μL of 120 mg / mL liver homogenate, 60 μL of 5 mg / mL NADPH, 10 μL of 0.2 mg / mL testosterone, and 10 μL of the test sample were mixed thoroughly and the absorbance A0 was measured. After incubation at 37℃ for 60 min, the absorbance A1 was measured to obtain the NADPH change value. The blank group was phosphate buffer (pH 7.4), and the experimental group was the test sample (30 mg / mL).

[0068] The calculation formula is I% = (△A1-△A0) / △A0×100%. The experiment was repeated 3 times, with 3 parallel samples each time.

[0069] Where △A1 represents the NADPH change value of the experimental group and the positive control group, and △A0 represents the NADPH change value of the blank group.

[0070] The specific test results are shown in Table 3.

[0071] Table 3 As shown in Table 3, the anti-hair loss shampoo of the present invention has an inhibition rate of over 80% on 5α-reductase activity, which fully demonstrates that the synergistic combination of the components of the product of the present invention can effectively penetrate into the scalp and hair follicles, thereby effectively inhibiting 5α-reductase activity, effectively inhibiting the conversion of testosterone into the hair loss factor dihydrotestosterone, reducing the accumulation of DHT in the scalp and hair follicles, and eliminating the damaging and antagonistic effect of DHT on hair follicles from the root cause of the disease.

[0072] Test Example 2: Human Efficacy Test Test samples: Samples provided in Examples 1-13 and Comparative Examples 1-6.

[0073] The clinical trial involved 152 volunteers aged 30-55 years with alopecia areata or baldness, with an equal number of men and women. The participants were randomly divided into 19 groups of 8 people each (half male and half female), and used the samples provided in Examples 1-13 and Comparative Examples 1-6, respectively.

[0074] Instructions for use: Wet the entire hair with the test sample at 25℃, massage for one or two minutes, then rinse the hair thoroughly with water and blow-dry. Use once every two nights for three consecutive months.

[0075] The efficacy evaluation criteria are shown in Table 4 below: Table 4 The test results are shown in Table 5 below: Table 5 As shown in Table 5, compared with Comparative Example 1, the anti-hair loss shampoo provided in Examples 1-3 of this invention, by adding Chlorella active ingredients, showed significant effects after use by volunteers and has strong practicality.

[0076] As can be seen from Examples 4-9 and Comparative Example 2, adding the anti-hair loss active composition to the product of the present invention can improve the anti-hair loss and hair growth effect, and caffeine, nicotinamide, panthenol, adenosine, acetyl tetrapeptide-3 and arborvitae leaf extract have synergistic effects at a specific mass ratio; As can be seen from Examples 10-13, the choice of surfactant also affects the hair loss prevention and hair growth efficacy of the product; As can be seen from Comparative Examples 3-6, the addition and selection of scalp barrier repair compositions also affect the efficacy of the products.

[0077] Test Example 3, Stability Evaluation Experiment The samples obtained in Examples 1-3 were stored at 4°C, 25°C and 45°C for 90 days, respectively, and the changes in appearance, color, odor, pH value and viscosity of the products were tested periodically.

[0078] The results show that: The samples did not exhibit significant stratification, precipitation, crystallization, abnormal color, or off-odor under any storage conditions, and the pH value and viscosity changes were all within the normal range, indicating that the product of this invention has good storage stability.

[0079] Test Example 4: Security Test The specific method was as follows: Forty women aged 18-35 years with no history of allergies were selected. Each subject used samples prepared according to Examples 1-13. After cleaning the subject's arm, approximately 0.020 mL-0.025 mL of test sample was added to the patch. The patch containing the sample was then applied to the selected location on the arm using non-irritating adhesive tape. After application, the patch was gently pressed with a finger to ensure even adhesion to the skin, and left for 48 hours. During these 48 hours, the patch area was kept dry, and subjects were advised to avoid strenuous exercise, scratching, and prolonged sun exposure. After 48 hours, the patch was removed and marked. The results were assessed under sufficient light after 30 minutes, once the pressure marks disappeared.

[0080] The grading criteria for adverse skin reactions are shown in Table 6: Table 6 The results are shown in Table 7: Table 7 As shown in Table 7, no adverse skin reactions were observed in the volunteers after the spot tester was removed. Therefore, the product described in this invention is safe and non-irritating, has no adverse reactions on the human body, and is highly safe.

[0081] The applicant declares that the technical solution of this invention is illustrated by the above embodiments, but this invention is not limited to the above embodiments, that is, it does not mean that this invention must rely on the above embodiments to be implemented. Those skilled in the art should understand that any improvements to this invention, equivalent substitutions of raw materials for the products of this invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of this invention.

[0082] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0083] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

Claims

1. A hair loss prevention shampoo containing Chlorella active ingredients, characterized in that, The components of the anti-hair loss shampoo containing Chlorella active ingredients include Chlorella active ingredients, an anti-hair loss active composition, a surfactant, a scalp barrier repair composition, and deionized water; The scalp barrier repair composition comprises a combination of ectoine, ceramide, and sodium hyaluronate.

2. The anti-hair loss shampoo containing Chlorella active ingredients as described in claim 1, characterized in that, By weight, the anti-hair loss shampoo containing Chlorella active ingredients comprises 0.1-5 parts Chlorella active ingredients, 0.1-6 parts anti-hair loss active composition, 5-25 parts surfactant, 0.05-2 parts scalp barrier repair composition, and 60-95 parts deionized water.

3. The anti-hair loss shampoo containing Chlorella active ingredients as described in claim 1 or 2, characterized in that, The surfactant includes any one or a combination of at least two of sodium laureth sulfate, cocamidopropyl betaine, sodium lauroyl sarcosinate, or sodium lauroyl methyl taurate. Preferably, the surfactant includes sodium lauryl ether sulfate, cocamidopropyl betaine, sodium lauroyl sarcosinate, and sodium lauroyl methyl taurate.

4. The anti-hair loss shampoo containing Chlorella active ingredients as described in any one of claims 1-3, characterized in that, The anti-hair loss active composition comprises a combination of at least two of caffeine, nicotinamide, panthenol, arborvitae leaf extract, adenosine, acetyl tetrapeptide-3, or biotin.

5. The anti-hair loss shampoo containing Chlorella active ingredients as described in claim 4, characterized in that, The anti-hair loss active composition is a combination of caffeine, nicotinamide, panthenol, adenosine, acetyl tetrapeptide-3, and arborvitae leaf extract.

6. The anti-hair loss shampoo containing Chlorella active ingredient as described in any one of claims 1-5, characterized in that, The mass ratio of ectoine, ceramide and sodium hyaluronate is (1-3):(0.1-1):(0.5-2).

7. The anti-hair loss shampoo containing Chlorella active ingredient as described in any one of claims 1-6, characterized in that, The anti-hair loss shampoo also includes excipients; Preferably, the excipients include any one or a combination of at least two of the following: pH adjuster, chelating agent, preservative, thickener or fragrance; Preferably, the excipients include any one or a combination of at least two of glycerin, citric acid, sodium chloride, sodium benzoate, or fragrance.

8. A method for preparing an anti-hair loss shampoo containing Chlorella active ingredients as described in any one of claims 1-7, characterized in that, The preparation method includes mixing Chlorella active ingredients, anti-hair loss active composition, surfactant and scalp barrier repair composition evenly in deionized water to obtain the anti-hair loss shampoo containing Chlorella active ingredients.

9. The preparation method according to claim 8, characterized in that, The preparation method includes: (1) Heat deionized water and add the anti-hair loss active composition, then mix well; (2) Then add the surfactants one by one and mix well; (3) After cooling, add Chlorella active ingredients and scalp barrier repair composition, mix evenly, add citric acid to adjust pH, then add sodium benzoate and fragrance, mix evenly to obtain the anti-hair loss shampoo containing Chlorella active ingredients.

10. The preparation method according to claim 9, characterized in that, The step (1) involves heating to 70-75℃; Preferably, the temperature is reduced to 40-45°C in step (3); Preferably, step (3) involves adjusting the pH to 5.5-6.5.

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