Schistosomiasis detection miracidia incubation observation device

Abstract

The utility model relates to the field of egg cultivation and detection, in particular to a schistosomiasis detection miracidia incubation observation device. The schistosomiasis detection miracidia incubation observation device is convenient to detect, economical and applicable. A schistosomiasis detection miracidia incubation observation device comprises a cultivation box, an observation part, observation mirrors, a base, a cultivation cup and the like, the observation part protruding upwards is arranged at the top of the cultivation box, three observation holes are formed in the upper side of the observation part at intervals, the observation mirrors capable of turning over to adjust the angle are arranged in the observation holes in an embedded mode, and the observation cups are arranged on the base. A base is arranged at the bottom in the cultivation box, and three cultivation cups are placed on the base at intervals. The observation background of the device is black, so that observation errors caused by factors such as color change of the observation background, weather change and insufficient light are avoided; and light contrast can be enhanced, so that an observer can see the characteristics of miracidia clearly, missing detection is avoided, and the miracidia detection device is convenient to detect, economical and practical.

Description

Technical Field

[0001] The utility model relates to the field of worm egg cultivation detection, in particular to a miracidium hatching observation device for detecting schistosomiasis. Background Art

[0002] Schistosomiasis is one of the major infectious diseases in my country. It is a zoonotic parasitic disease that seriously endangers human health, hinders and affects the social and economic development of epidemic areas, and is contagious, endemic and naturally zoonotic. The diagnostic methods of schistosomiasis mainly include etiological diagnosis and immunological diagnosis. The immunological diagnosis method mainly detects serum antibodies and antigens, which are mainly used as patient screening and auxiliary diagnosis methods. It cannot distinguish between current infection, past infection and identification of worm stages. Etiological diagnosis is the gold standard for the diagnosis of schistosomiasis patients. The etiological diagnosis of schistosomiasis is mainly fecal examination, and the commonly used methods are egg collection and hatching method and kato-katz method. The latter is to find eggs through direct microscopic examination, but due to the uneven distribution of eggs in feces and limited sampling volume, it is easy to miss the diagnosis of low- and moderately infected people or animals. The egg collection and hatching method has a higher detection rate than the kato-katz method due to the large sampling volume during the test. Therefore, it is currently the main method for schistosomiasis diagnosis.

[0003] When conducting egg collection and incubation methods to detect schistosomiasis, the currently commonly used top tube incubation method and nylon silk egg collection and incubation method both require observing whether the eggs have hatched into miracidia to determine the results. Schistosoma miracidia are only the size of a pinpoint (about 99μm×35μm), and conventional observation requires the naked eye. In recent years, miracidia microscopes and miracidia dynamic automatic identification systems (devices) developed by domestic research institutions have improved the detection effect, but they all require special equipment and are expensive, making them difficult to popularize at the grassroots level. Usually, the results are observed with the help of auxiliary magnifying glasses, black paper, flashlights, etc., which is inconvenient to operate. At the same time, the appropriate temperature, light intensity, and projection angle all affect the result determination.

[0004] According to the document "Healthy Hubei Action (2020-2030)", all schistosomiasis-endemic counties in the province will reach the elimination standard by 2030. In order to improve the level of risk monitoring work, ensure the quality of risk monitoring work, improve sensitivity and quality, detect schistosomiasis epidemics and potential risks of snails, and take timely intervention measures. Therefore, a convenient and economical wild feces monitoring and detection technology is urgently needed to assist the hatching method for miracidium observation. Utility Model Content

[0005] In order to overcome the defects in the above-mentioned background technology, the technical problem to be solved by the utility model is to provide a schistosomiasis detection miracidium hatching observation device. The technical solution of the utility model is to provide a schistosomiasis detection miracidium hatching observation device which is convenient for detection and economical.

[0006] The technical solution of the utility model is: a schistosomiasis detection miracidium hatching observation device, including an incubator, a base, a heating lamp, a temperature sensor and a display screen, the front and rear side walls inside the incubator are respectively provided with heating lamps, the bottom of the incubator is provided with a base, the middle of the base is provided with a temperature sensor, the left front side of the incubator is provided with a display screen, and the temperature sensor and the heating lamp are electrically connected to the display screen, and also include an observation part, an observation mirror, a incubator cup and a plugging cover, the top of the incubator is provided with an upwardly protruding observation part, the upper side of the observation part is provided with three observation holes spaced apart, each of the observation holes is embedded with an observation mirror capable of flipping and adjusting the angle, three incubator cups are placed at intervals on the base, a discharge hole is provided on the right side of the incubator, and a plugging cover is clamped in the discharge hole.

[0007] Furthermore, the observation mirror is located obliquely above the culture cup.

[0008] Furthermore, the culture cup is configured as a tapered structure that is wider at the top and narrower at the bottom.

[0009] Furthermore, it also includes a sealing ring, and the edge of each observation hole is embedded with a sealing ring, and the sealing ring is respectively fitted with the corresponding observation mirror.

[0010] Furthermore, the schistosomiasis detection miracidium hatching observation device also includes a ventilation component, which includes a ventilator, a protrusion and a sealing cover. The ventilator is embedded in the left side of the incubator, and protrusions are circumferentially spaced on the outer side of the ventilator casing. The sealing cover is clamped on the outer side of the ventilator casing, and the inner edge of the sealing cover is provided with slots that are compatible with the protrusions.

[0011] Furthermore, the ventilator and the discharge hole are maintained on the same horizontal axis.

[0012] Compared with the prior art, the utility model has the following advantages: the observation background of the device is black, which avoids observation errors caused by factors such as changes in background color, weather changes, and insufficient light; and can enhance light contrast so that the observer can see the characteristics of the miracidia more clearly to avoid missed detection, which is not only convenient for detection but also economical and practical; by adding different proportions of liquid, eggs and intermediate hosts into three groups of culture cups through the discharge hole, so that during the culture of eggs, the observation mirror can be flipped and adjusted to observe the growth of eggs in the corresponding culture cups in real time, which is convenient for recording and detecting eggs at any time; for this purpose, three groups of eggs with different density ratios are cultured in the same ambient temperature, and the eggs can be viewed in real time, which is more conducive to improving the accuracy of experimental data. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1It is a three-dimensional structural schematic diagram of the utility model.

[0014] Figure 2 This is a structural separation diagram of the sealing cover, the blocking cover and the incubator of the utility model.

[0015] Figure 3 It is a top view of the observation mirror of the utility model in the flipped-up state.

[0016] Figure 4 It is a cross-sectional view of the utility model.

[0017] Figure 5 This is a structural separation diagram of the ventilator and sealing cover of the utility model.

[0018] Among them, the above-mentioned drawings include the following figure marks: 1. incubation box, 2. observation part, 20. observation hole, 21. observation mirror, 22. sealing ring, 3. base, 4. incubation cup, 5. heating lamp, 6. temperature sensor, 7. display screen, 8. ventilator, 80. bump, 81. sealing cover, 82. slot, 83. plugging cover, a. discharge hole. DETAILED DESCRIPTION

[0019] The utility model is further described below in conjunction with the accompanying drawings and embodiments.

[0020] Embodiment 1: A schistosomiasis detection miracidium hatching observation device provided by the present invention, see Figure 1-Figure 4As shown, it includes a cultivation box 1, an observation part 2, an observation mirror 21, a base 3, a cultivation cup 4, a heating lamp 5, a temperature sensor 6, a display screen 7 and a plug cover 83. The top of the cultivation box 1 is provided with an upwardly protruding observation part 2, the observation part 2 is inclined, and three observation holes 20 are spaced apart on the upper side of the observation part 2. Each observation hole 20 is embedded with a rotatable observation mirror 21. The observation mirror 21 can be a magnifying glass or other optical lens, which is convenient for assisting experimenters to detect and view insect eggs in real time, and the observation mirror 21 can be flipped to adjust the angle, which is more convenient to use. The bottom of the cultivation box 1 is provided with a base 3, and three holes are spaced apart on the base 3. Cultivation cups 4 are placed in the three holes at the same time. The cultivation cups 4 are made of transparent glass, and the lower diameter The incubator 1 has a large size and a small upper diameter, which is beneficial to improving the experimental personnel's observation field of view of the eggs in the cultivation cup 4, and the observation mirror 21 is located obliquely above the cultivation cup 4, which is beneficial to conveniently and clearly viewing the growth of the eggs in the corresponding cultivation cup 4. The front and rear side walls inside the incubator 1 are respectively provided with heating lamps 5, and the heating temperature of the heating lamps 5 can be adjusted arbitrarily. A temperature sensor 6 is provided in the middle of the base 3, and a display screen 7 is provided on the left front side of the incubator 1. The temperature sensor 6 and the heating lamp 5 are electrically connected to the display screen 7. The incubator 1 can be heated by the heating lamp 5, and the ambient temperature of the incubator 1 can be stably controlled by the temperature sensor 6. A discharge hole a is provided on the right side of the incubator 1, and a plug cover 83 is clamped in the discharge hole a.

[0021] For further information, see Figure 1-Figure 4 As shown, a sealing ring 22 is also included. The edge of each observation hole 20 is embedded with a sealing ring 22, and the sealing ring 22 is respectively fitted with the corresponding observation mirror 21, which is conducive to forming a closed environmental space in the incubator 1 behind the observation mirror 21.

[0022] The observation background of the device is black, which can avoid observation errors caused by factors such as changes in the background color, weather changes, and insufficient light. The device can also enhance light contrast by adjusting the brightness of the heat lamp 5, so that the observer can see the characteristics of the miracidium more clearly. When using the incubator 1, first remove the plugging cover 83, and add different proportions of liquid, eggs and intermediate hosts (which can be snails) to the three incubation cups 4 placed on the base 3 through the discharge hole a. After the above operation is completed, reset the plugging cover 83, input corresponding parameters on the display screen 7, turn on the heating lamp 5, and use the auxiliary effect of the temperature sensor 6 to control the temperature inside the incubator 1 between 26 and 30 degrees Celsius, which is conducive to providing a suitable incubation temperature for the eggs. In this process, the eggs in the incubation cup 4 can be observed and detected every 4-6 hours through the observation mirror 21, and the corresponding data can be recorded. In this way, the three groups of eggs with different densities are placed in the same ambient temperature for incubation and detection, which is conducive to improving the accuracy of the experimental data. The device is not only convenient for detection but also economical and applicable.

[0023] Embodiment 2: Based on Embodiment 1, refer to Figure 1-Figure 5 As shown, a ventilation assembly is also included, which includes a ventilator 8, a protrusion 80 and a sealing cover 81. The ventilator 8 is embedded in the left side of the incubator 1, and the ventilator 8 and the discharge hole a are kept on the same horizontal axis, which is beneficial for extracting the air inside the incubator 1 through the ventilator 8 so that fresh air can be sucked in from the discharge hole a, thereby helping to improve the ventilation efficiency. The outer side of the casing of the ventilator 8 is provided with protrusions 80 at intervals along the circumferential direction, and the outer side of the casing of the ventilator 8 is clamped with a sealing cover 81, and the inner edge of the sealing cover 81 is provided with slots 82 that are compatible with the protrusions 80 at intervals.

[0024] When the interior of the incubator 1 needs to be ventilated, the sealing cover 81 and the blocking cover 83 can be removed respectively, and the ventilator 8 can be started to extract the air inside the incubator 1, so that fresh air can be sucked in from the discharge hole a, thereby achieving the purpose of ventilating the interior of the incubator 1.

[0025] The above embodiments are only for illustrating the technical concept and features of the utility model, and their purpose is to enable people familiar with the technology to understand the content of the utility model and implement it accordingly, and they cannot be used to limit the protection scope of the utility model. Any equivalent changes or modifications made according to the spirit of the utility model should be included in the protection scope of the utility model.

Claims

1. A schistosomiasis detection miracidium hatching observation device, comprising an incubator (1), a base (3), a heating lamp (5), a temperature sensor (6) and a display screen (7), wherein the front and rear side walls of the incubator (1) are respectively provided with heating lamps (5), the bottom of the incubator (1) is provided with a base (3), the middle of the base (3) is provided with a temperature sensor (6), the left front side of the incubator (1) is provided with a display screen (7), and the temperature sensor (6) and the heating lamp (5) are both electrically connected to the display screen (7), characterized in that: The incubator (1) also comprises an observation portion (2), an observation mirror (21), a cultivation cup (4) and a plugging cover (83). The top of the incubator (1) is provided with an upwardly protruding observation portion (2). Three observation holes (20) are spaced apart on the upper side of the observation portion (2). Each of the observation holes (20) is embedded with an observation mirror (21) capable of being flipped and adjusted in angle. Three cultivation cups (4) are spaced apart on the base (3). A discharge hole (a) is provided on the right side of the incubator (1), and a plugging cover (83) is snapped into the discharge hole (a).

2. A schistosomiasis detection miracidium hatching observation device as claimed in claim 1, characterized in that: The observation mirror (21) is located obliquely above the cultivation cup (4).

3. The device for observing the hatching of miracidium for detecting schistosomiasis according to claim 1, characterized in that: The cultivation cup (4) is arranged in a conical structure that is wide at the top and narrow at the bottom.

4. The device for observing the hatching of miracidium for detecting schistosomiasis according to claim 1, characterized in that: It also includes a sealing ring (22). The edge of each observation hole (20) is embedded with a sealing ring (22), and the sealing ring (22) is respectively fitted with the corresponding observation mirror (21).

5. The device for observing the hatching of miracidium for detecting schistosomiasis according to claim 1, characterized in that: The schistosomiasis detection miracidium hatching observation device also includes a ventilation assembly, the ventilation assembly including a ventilator (8), a protrusion (80) and a sealing cover (81), the ventilator (8) being embedded on the left side of the incubator (1), the protrusions (80) being arranged at intervals along the circumferential direction on the outer side of the housing of the ventilator (8), the sealing cover (81) being clamped on the outer side of the housing of the ventilator (8), and the inner edge of the sealing cover (81) being provided with clamping grooves (82) adapted to the protrusions (80) at intervals.

6. The device for observing the hatching of miracidium for detecting schistosomiasis according to claim 5, characterized in that: The ventilator (8) and the discharge hole (a) are maintained on the same horizontal axis.

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