Parallel chromatographic column system

Abstract

The utility model relates to a parallel chromatographic column system which comprises at least two chromatographic columns, each chromatographic column is at least provided with a first port located at the top of the chromatographic column and a second port located at the bottom of the chromatographic column, the first ports of the two chromatographic columns are respectively connected with a first main pipeline through a first branch pipeline in an on-off mode, and the second ports of the two chromatographic columns are respectively connected with a second main pipeline through a second branch pipeline in an on-off mode. The first main pipeline is used for being connected with a chromatography system in an on-off manner; second ports of the two chromatographic columns are respectively connected with a second main pipeline through a second branch pipeline in an on-off manner, and the second main pipeline is used for being connected with the chromatographic system in an on-off manner, so that the two chromatographic columns are connected into the chromatographic system in parallel; the flushing pipeline is connected between the first main pipeline and the second main pipeline in an on-off mode so that a recyclable cleaning flow path can be formed between the first main pipeline and the second main pipeline. According to the utility model, the productivity of the chromatographic system can be expanded, and ideal material purity and chromatographic column cleaning effect can be achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of separation and purification, in particular to a parallel chromatography column system. Background Art

[0002] At present, chromatography equipment is used in the separation and purification process of a class of protein drugs in the pharmaceutical industry, as well as in the separation and purification process of lactoferrin, α and β proteins in dairy milk.

[0003] In order to improve the efficiency of the chromatography column, two chromatography columns can be connected in parallel to the chromatography system to achieve simultaneous operation of the two chromatography columns and expand production capacity. However, since each chromatography column of this type of parallel chromatography column needs to be split, the cleaning after the split may have problems such as insufficient flow rate and poor cleaning effect in the subsequent cleaning process.

[0004] Therefore, the inventors, relying on their many years of experience and practice in related industries, propose a parallel chromatography column system to overcome the defects of the prior art. Utility Model Content

[0005] The utility model aims to provide a parallel chromatography column system, which can realize the expansion of the production capacity of the chromatography system and achieve the ideal material purity and chromatography column cleaning effect while using a small amount of balancing liquid, eluent and regeneration liquid.

[0006] The purpose of the utility model can be achieved by adopting the following scheme:

[0007] The utility model provides a parallel chromatography column system, which comprises:

[0008] At least two chromatography columns, each of the chromatography columns having at least a first port located at the top thereof and a second port located at the bottom thereof, the first ports of the two chromatography columns being respectively connected to a first main pipe in an on-off manner through a first branch pipe, the first main pipe being used to be connected to a chromatography system in an on-off manner; the second ports of the two chromatography columns being respectively connected to a second main pipe in an on-off manner through a second branch pipe, the second main pipe being used to be connected to the chromatography system in an on-off manner, so that the two chromatography columns are connected to the chromatography system in parallel;

[0009] A flushing pipeline is connected between the first main pipeline and the second main pipeline in an on-off manner to form a circulatable cleaning flow path between the first main pipeline and the second main pipeline.

[0010] In a preferred embodiment of the present invention, an exhaust valve is provided on the flushing pipeline to exhaust the residual gas in the parallel chromatography column system.

[0011] In a preferred embodiment of the present invention, a first main valve for controlling the on-off of the first main pipeline is provided on the first main pipeline, and a first regulating valve and a second regulating valve for controlling flow distribution are respectively provided on the two first branch pipelines.

[0012] In a preferred embodiment of the present invention, a first reducer is provided on the first main pipe and between the first main valve and the chromatography system, and the inner diameter of the first reducer gradually increases from the chromatography system to the first main valve.

[0013] In a preferred embodiment of the present invention, a second reducer is provided on the two first branch pipes and between the first main pipe and the first regulating valve and the second regulating valve, respectively, and the inner diameter of the second reducer gradually increases from the first main pipe to the first regulating valve or from the first main pipe to the second regulating valve.

[0014] In a preferred embodiment of the present invention, a first on-off valve and a second on-off valve are respectively provided on the two first branch pipelines.

[0015] In a preferred embodiment of the present invention, a first flow meter is provided on the first main pipeline, and a second flow meter is provided on at least one of the two first branch pipelines.

[0016] In a preferred embodiment of the present invention, a second main valve for controlling the on-off of the second main pipe is provided on the second main pipe, and a third regulating valve and a fourth regulating valve for controlling flow distribution are respectively provided on the two second branch pipes.

[0017] In a preferred embodiment of the present invention, a third reducer is provided on the second main pipe and between the second main valve and the chromatography system, and the inner diameter of the third reducer gradually increases from the chromatography system to the second main valve.

[0018] In a preferred embodiment of the present invention, fourth reducers are respectively provided on the two second branch pipelines and between the second main pipeline and the third regulating valve and the fourth regulating valve, and the inner diameter of the fourth reducer gradually increases from the second main pipeline to the third regulating valve or from the second main pipeline to the fourth regulating valve.

[0019] In a preferred embodiment of the present invention, a third flow meter is provided on the second main pipeline, and a fourth flow meter is provided on at least one of the two second branch pipelines.

[0020] In a preferred embodiment of the present utility model, the flushing pipeline is provided with a fifth flow meter and a twin-screw pump capable of driving the liquid in the flushing pipeline to flow in both directions.

[0021] In a preferred embodiment of the present invention, at least a portion of the flushing pipeline is connected between the first main valve and the second main valve.

[0022] From the above, the characteristics and advantages of the parallel chromatography column system of the utility model are:

[0023] The parallel chromatography column system comprises at least two chromatography columns, the first ports of the two chromatography columns are connected to the chromatography system in a switchable manner through the corresponding first branch pipeline and the first main pipeline in turn, and the second ports of the two chromatography columns are connected to the chromatography system in a switchable manner through the corresponding second branch pipeline and the second main pipeline in turn, so that the two chromatography columns are connected to the chromatography system in parallel, and during the balancing, loading or elution process, the material or cleaning liquid output by the chromatography system can enter the two chromatography columns respectively through the first main pipeline, the two first branch pipelines and the first ports of the two chromatography columns for washing. The materials are separated, purified or cleaned, and then returned to the chromatography system through the second ports of the two chromatography columns, the two second branch pipes and the second main pipe, so as to realize the forward equilibration, sample loading and de-elution process of the parallel chromatography columns; in addition, the second main pipe, the two second branch pipes and the second ports of the two chromatography columns can respectively enter the two chromatography columns for separation, purification or cleaning of the materials, and then returned to the chromatography system through the first ports of the two chromatography columns, the two first branch pipes and the first main pipe, so as to realize the reverse equilibration, sample loading and de-elution process of the parallel chromatography columns;

[0024] A flushing pipeline is connected between the first main pipeline and the second main pipeline. After completing the conventional balancing, loading and de-washing processes, the residual liquid and gas in the parallel chromatography column system can be discharged through the flushing pipeline, and a local circulation cleaning flow path is formed between the first main pipeline and the second main pipeline through the flushing pipeline to achieve the purpose of local cleaning. While realizing the expansion of the production capacity of the chromatography system, it can achieve ideal material purity and chromatography column cleaning effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The following drawings are only intended to illustrate and explain the present invention, and do not limit the scope of the present invention.

[0026] Figure 1 : It is a structural schematic diagram of the utility model parallel chromatography column system.

[0027] The accompanying drawings in the present utility model are as follows:

[0028] 1. Chromatography column; 2. Chromatography system;

[0029] 201. forward and reverse flow control device; 2011. first connecting pipeline;

[0030] 2012, second connecting pipeline; 2013, third connecting pipeline;

[0031] 2014, fourth connecting pipeline; 2015, first valve member;

[0032] 2016, second valve component; 2017, third valve component;

[0033] 2018, the fourth valve; 2019, the fifth valve;

[0034] 2020, sixth valve member; 202, liquid supply pipeline;

[0035] 3. The first main pipeline; 4. The first branch pipeline;

[0036] 5. Second main pipeline; 6. Second branch pipeline;

[0037] 7. Flushing pipeline; 8. First main valve;

[0038] 9. The first on-off valve; 10. The first regulating valve;

[0039] 11. Second regulating valve; 12. Second on-off valve;

[0040] 13. A first flow meter; 14. A second flow meter;

[0041] 15. First reducer; 16. Second main valve;

[0042] 17. The third regulating valve; 18. The fourth regulating valve;

[0043] 19. The third flow meter; 20. The fourth flow meter;

[0044] 21. Second reducer pipe; 22. Fourth reducer pipe;

[0045] 23. The third reducer pipe; 24. Twin-screw pump;

[0046] 25. The fifth flow meter; 26. The external exhaust valve. DETAILED DESCRIPTION

[0047] In order to have a clearer understanding of the technical features, purposes and effects of the utility model, the specific implementation methods of the utility model are now described with reference to the accompanying drawings.

[0048] like Figure 1As shown, the utility model provides a parallel chromatography column system, which includes at least two chromatography columns 1, each chromatography column 1 having at least a first port located at the top and a second port located at the bottom, the first ports of the two chromatography columns 1 are respectively connected to the first main channel 3 through a first branch pipeline 4, and the first main channel 3 is used to be connected to the chromatography system 2; the second ports of the two chromatography columns 1 are respectively connected to the second main channel 5 through a second branch pipeline 6, and the second main channel 5 is used to be connected to the chromatography system 2, so that the two chromatography columns 1 are connected to the chromatography system 2 in parallel; a flushing pipeline 7, the flushing pipeline 7 is connected between the first main channel 3 and the second main channel 5 in an on-off manner, so as to form a circulatable cleaning flow path between the first main channel 3 and the second main channel 5.

[0049] In the utility model, the parallel chromatography column system comprises at least two chromatography columns 1, the first ports of the two chromatography columns 1 are connected to the chromatography system 2 in a switchable manner through the corresponding first branch pipeline 4 and the first main pipeline 3, and the second ports of the two chromatography columns 1 are connected to the chromatography system 2 in a switchable manner through the corresponding second branch pipeline 6 and the second main pipeline 5, so that the two chromatography columns 1 are connected to the chromatography system 2 in parallel. During the balancing, loading or elution process, the material or cleaning liquid output by the chromatography system 2 can enter the two chromatography columns 1 through the first main pipeline 3, the two first branch pipelines 4 and the first ports of the two chromatography columns 1 respectively to separate and purify or clean the material, and then return to the chromatography system 2 through the second ports of the two chromatography columns 1, the two second branch pipelines 6 and the second main pipeline 5, so as to realize the forward balancing, loading and elution process of the parallel chromatography columns 1; in addition, the second main pipeline 5, the two second branch pipelines 6 and the second main pipeline 5 can also be used to connect the two chromatography columns 1 in a switchable manner. The pipeline 6 and the second ports of the two chromatography columns 1 respectively enter the two chromatography columns 1 to separate, purify or clean the materials, and then return to the chromatography system 2 through the first ports of the two chromatography columns 1, the two first branch pipelines 4 and the first main pipeline 3, to realize the reverse balance, loading and de-elution process of the parallel chromatography columns 1, and according to different requirements, the forward flow circulation or reverse flow circulation of the parallel chromatography columns 1 is carried out; in addition, a flushing pipeline 7 is connected between the first main pipeline 3 and the second main pipeline 5. After completing the conventional balance, loading and de-elution process, the residual liquid and gas in the parallel chromatography column system can be discharged through the flushing pipeline 7, and a local circulation cleaning flow path is formed between the first main pipeline 3 and the second main pipeline 5 through the flushing pipeline 7 to achieve the purpose of local cleaning. While realizing the expansion of the production capacity of the chromatography system 2, it can achieve the ideal material purity and the cleaning effect of the chromatography column.

[0050] In the present invention, the chromatography system 2 used is an existing analytical instrument for protein and polypeptide purification, and the specific structure of the chromatography system 2 is not limited herein.

[0051] In the present invention, Figure 1As shown, the two chromatography columns 1 in the parallel chromatography columns 1 are axial chromatography columns. Of course, the two chromatography columns 1 can also be radial chromatography columns, or one of the two chromatography columns 1 can be an axial chromatography column and the other can be a radial chromatography column.

[0052] In an optional embodiment of the present invention, Figure 1 As shown, an external discharge valve 26 is provided on the flushing pipeline 7, and the residual gas in the parallel chromatography column system can be discharged by opening the external discharge valve 26.

[0053] In an optional embodiment of the present invention, Figure 1 As shown, the first main pipeline 3 is provided with a first main valve 8 for controlling the on-off of the first main pipeline 3, and the two first branch pipelines 4 are respectively provided with a first regulating valve 10 and a second regulating valve 11 for controlling flow distribution. During the forward balance, sample loading and de-elution processes, the flow of liquid entering the two chromatography columns 1 can be respectively controlled by the first regulating valve 10 and the second regulating valve 11.

[0054] Further, such as Figure 1 As shown, a first reducing pipe 15 is provided on the first main pipeline 3 and between the first main valve 8 and the chromatography system 2, and the inner diameter of the first reducing pipe 15 gradually increases from the chromatography system 2 to the first main valve 8. Second reducing pipes 21 are provided on the two first branch pipelines 4 and between the first main pipeline 3 and the first regulating valve 10 and the second regulating valve 11, respectively, and the inner diameter of the second reducing pipe 21 gradually increases from the first main pipeline 3 to the first regulating valve 10 or from the first main pipeline 3 to the second regulating valve 11.

[0055] Further, such as Figure 1 As shown, the two first branch pipelines 4 are respectively provided with a first on-off valve 9 and a second on-off valve 12. During the forward balancing, loading and elution processes, the on-off states of the two first branch pipelines 4 can be controlled by the first on-off valve 9 and the second on-off valve 12 respectively.

[0056] Further, such as Figure 1 As shown, a first flow meter 13 is provided on the first main pipeline 3, and a second flow meter 14 is provided on at least one of the two first branch pipelines 4. During the forward balance, sample loading and elution processes, the total flow in the first main pipeline 3 is measured by the first flow meter 13, and the flow in one of the first branch pipelines 4 is measured by the second flow meter 14, so that the flow in the other first branch pipeline 4 can be obtained, and then the flow distribution of the liquid in the two parallel chromatography columns 1 can be known.

[0057] In the forward balance, sample loading and de-elution process of the utility model, the material enters the two chromatography columns 1 from the chromatography system 2 through the first main pipeline 3, the two first branch pipelines 4 and the first ports on the tops of the two chromatography columns 1 in sequence. In this process, the liquid passes through the first reducer 15 and the second reducer 21 (the first reducer 15 and the second reducer 21 are reducers with gradually increasing diameters according to the number of parallel chromatography columns 1, and the first reducer 15 and the second reducer 21 are arranged to obtain high-flow cleaning, so that the pipeline from the first main valve 8 to the first regulating valve 10 and the second regulating valve 11 will not be caused by high-flow flow restriction. The pressure drop suddenly increases, and at the same time, the cleaning flow rate of this section of thickened pipe can meet the cleaning requirement of 1.5m / s of GMP sanitary design). The flow rate of the liquid distributed to the two first branch pipes 4 can be known through the first flow meter 13 and the second flow meter 14, and the flow rate distributed to the two chromatography columns 1 is adjusted by the first regulating valve 10 and the second regulating valve 11 respectively. After the fluid is adsorbed by the two chromatography columns 1, the remaining fluid flows out from the second port at the bottom of the two chromatography columns 1, and then flows back to the chromatography system 2 through the two second branch pipes 6 and the first branch pipe 4 in turn, thereby realizing the forward balance, loading and elution processes of the two parallel chromatography columns 1.

[0058] In an optional embodiment of the present invention, Figure 1 As shown, the second main pipeline 5 is provided with a second main valve 16 for controlling the on-off of the second main pipeline 5, and the two second branch pipelines 6 are respectively provided with a third regulating valve 17 and a fourth regulating valve 18 for controlling flow distribution. During the reverse balance, sample loading and de-elution processes, the third regulating valve 17 and the fourth regulating valve 18 can be used to control the flow of liquid entering the two chromatography columns 1 respectively.

[0059] Further, such as Figure 1 As shown, a third reducing pipe 23 is provided on the second main pipeline 5 and between the second main valve 16 and the chromatography system 2, and the inner diameter of the third reducing pipe 23 gradually increases from the chromatography system 2 to the second main valve 16. Fourth reducing pipes 22 are provided on the two second branch pipelines 6 and between the second main pipeline 5 and the third regulating valve 17 and the fourth regulating valve 18, respectively, and the inner diameter of the fourth reducing pipe 22 gradually increases from the second main pipeline 5 to the third regulating valve 17 or from the second main pipeline 5 to the fourth regulating valve 18.

[0060] In the present invention, the first reducer pipe 15, the second reducer pipe 21, the third reducer pipe 23 and the fourth reducer pipe 22 are conical cylindrical hollow pipes, which can be made of stainless steel. The reducer connecting pipe is an existing reducer connecting element (also called "reducer"), which is used when the nominal diameters of the valve and the pipeline (or the pipelines) are inconsistent, and the valve and the pipeline (or the pipelines) cannot be directly connected or welded through standard flanges or threads. At this time, the valve and the pipeline (or the pipelines) can be connected by adding a reducer connecting pipe.

[0061] Further, such as Figure 1 As shown, a third flow meter 19 is provided on the second main pipeline 5, and a fourth flow meter 20 is provided on at least one of the two second branch pipelines 6. During the reverse balance, sample loading and elution processes, the total flow in the second main pipeline 5 is measured by the third flow meter 19, and the flow in one of the second branch pipelines 6 is measured by the fourth flow meter 20, so that the flow in the other second branch pipeline 6 can be obtained, and then the flow distribution of the liquid in the two parallel chromatography columns 1 can be known.

[0062] In the reverse balancing, loading and washing processes of the utility model, since the flow direction of the material is opposite, the first flow meter 13 and the second flow meter 14 used in the forward direction cannot display the numerical values ​​normally in the reverse flow. Therefore, it is necessary to set the third flow meter 19 and the fourth flow meter 20 to obtain the flow distribution data. The other processes are opposite to those in the forward direction and will not be repeated here.

[0063] In an optional embodiment of the present invention, Figure 1 As shown, at least part of the flushing pipeline 7 is connected between the first main valve 8 and the second main valve 16, and a fifth flow meter 25 and a twin-screw pump 24 capable of driving the bidirectional flow of the liquid in the flushing pipeline 7 are provided on the flushing pipeline 7. The twin-screw pump 24 can realize forward and reverse adjustment, so as to change the reversal of the cleaning liquid in the local circulation cleaning process in the flushing pipeline 7. Among them, the twin-screw pump 24 can also be replaced by other two driving pumps (one forward and one reverse), and the other driving pumps can be but not limited to centrifugal pumps, rotor pumps, self-priming centrifugal pumps, etc.

[0064] In another optional embodiment of the present invention, Figure 1As shown, the chromatography system 2 includes a liquid supply pipeline 202 for supplying materials or cleaning liquid and a forward and reverse flow control device 201 for controlling the chromatography system 2 to output materials or cleaning liquid in forward and reverse directions. The forward and reverse flow control device 201 includes a first connecting pipeline 2011, a second connecting pipeline 2012, a third connecting pipeline 2013 and a fourth connecting pipeline 2014. One end of the first connecting pipeline 2011 and one end of the second connecting pipeline 2012 are both connected to the liquid supply pipeline 202, and along the flow direction of the liquid (material or cleaning liquid) in the liquid supply pipeline 202, the connection position of the first connecting pipeline 2011 and the liquid supply pipeline 202 is located upstream of the connection position of the second connecting pipeline 2012 and the liquid supply pipeline 202. The other end of the first connecting pipeline 2011 and the other end of the second connecting pipeline 2012 are both connected to the first main pipeline 3. A first valve component 2015 is provided on the first connecting pipeline 2011, and a second valve component 2016 is provided on the second connecting pipeline 2012; one end of the third connecting pipeline 2013 and the fourth connecting pipeline 2014 are connected to the first main pipeline 3. One end of each of the third connecting pipelines 2013 and 2014 is connected to the liquid supply pipeline 202, and along the flow direction of the liquid (material or cleaning liquid) in the liquid supply pipeline 202, the connection position of the third connecting pipeline 2013 and the liquid supply pipeline 202 is located downstream of the connection position of the fourth connecting pipeline 2014 and the liquid supply pipeline 202, the connection position of the third connecting pipeline 2013 and the liquid supply pipeline 202 is located downstream of the connection position of the first connecting pipeline 2011 and the liquid supply pipeline 202, and the connection position of the fourth connecting pipeline 2014 and the liquid supply pipeline 202 is located upstream of the connection position of the second connecting pipeline 2012 and the liquid supply pipeline 202, the other end of the third connecting pipeline 2013 and the other end of the fourth connecting pipeline 2014 are both connected to the second main pipeline 5, the third connecting pipeline 2013 is provided with a third valve component 2017, and the fourth connecting pipeline 2014 is provided with a fourth valve component 2018. In the actual working process, the second valve component 2016 and the fourth valve component 2018 can be closed, the first valve component 2015 and the third valve component 2017 can be opened, and the liquid flows through the liquid supply pipeline 202 and the first connecting pipeline 2011 in sequence and then enters the first main pipeline 3 to perform forward balance, sample loading, and de-elution of the parallel chromatography column 1. After the separation, purification or cleaning of the material is completed, the liquid flows through the second main pipeline 5 and the third connecting pipeline 2013 in sequence and then returns to the liquid supply pipeline 202; in addition, the first valve component 201 can be closed. 5 and the third valve component 2017, the second valve component 2016 and the fourth valve component 2018 are connected, and the liquid flows through the liquid supply pipeline 202 and the fourth connecting pipeline 2014 in sequence and then enters the second main pipeline 5, and performs reverse balancing, loading, and dewashing of the parallel chromatography column 1. After completing the separation, purification or cleaning of the material, the liquid flows through the first main pipeline 3 and the second connecting pipeline 2012 in sequence and then returns to the liquid supply pipeline 202, so that the parallel chromatography column 1 can be controlled to perform forward flow circulation or reverse flow circulation according to different requirements.

[0065] In this embodiment, if Figure 1 As shown, a fifth valve component 2019 is provided on the first main line 3, and a sixth valve component 2020 is provided on the second main line 5. By controlling the conduction or shutoff of the fifth valve component 2019 and the sixth valve component 2020, the on-off state of the first main line 3 and the second main line 5 can be controlled respectively.

[0066] In the present invention, the first valve component 2015, the second valve component 2016, the third valve component 2017, the fourth valve component 2018, the fifth valve component 2019 and the sixth valve component 2020 can all be on-off valves for controlling the on-off state of the connecting pipeline.

[0067] The parallel chromatography column system of the utility model complies with the food hygiene design standards, the metals that can directly contact with food are all made of 304L and above stainless steel, and the materials of the sealing parts are EPDM (ethylene propylene diene monomer rubber), silicone, Teflon, etc., which can improve the hygiene and safety of food.

[0068] In the cleaning process of the utility model, after the various impurities in the chromatography column 1 are washed away by the cleaning liquid, the chromatography column 1 is then washed with water at high intensity. The following cycle cleaning process is specifically adopted to achieve the purpose of cleaning and regenerating the chromatography column 1 while saving the regeneration liquid to the maximum extent. The details are as follows:

[0069] If a cleaning method is adopted in which the cleaning liquid enters the chromatography column 1 from the top and exits from the bottom, the cleaning liquid enters the two chromatography columns 1 respectively through the first main line 3, the two first branch lines 4 and the first ports on the tops of the two chromatography columns 1 in sequence. The flow rate of the cleaning liquid allocated to the two first branch lines 4 can be obtained through the first flow meter 13 and the second flow meter 14, and the flow rate allocated to the two chromatography columns 1 is adjusted by the first regulating valve 10 and the second regulating valve 11 respectively. In the process of flowing through the two chromatography columns 1, the cleaning liquid cleans the substances remaining in the chromatography columns 1, flows out from the second port of the chromatography column 1 after cleaning, and then flows back to the chromatography system 2 through the two second branch lines 6 and the second main line 5 in sequence, thereby realizing large-circulation cleaning of the parallel chromatography column system. In this process, a small circulation cleaning is formed by the flushing pipeline 7, the twin-screw pump 24 and the two chromatography columns 1, so as to achieve the purpose of high-flow rate cleaning of the chromatography column 1. The specific circulation process is: the cleaning liquid passes through the first main valve 8, the twin-screw pump 24, the second main valve 16 and the external discharge valve 26 in turn, so as to discharge the residual liquid and / or gas in the system, so that the cleaning liquid replaces the residual liquid and / or gas in the previous chromatography process in the system (because the chromatography column 1 requires that no gas can enter, problems such as dry filler glue will occur after air intake, resulting in poor column efficiency); then the external discharge valve 26 is closed, and the cleaning liquid passes through the second main valve 16, the twin-screw pump 24, the first main valve 8, the first regulating valve 10 and the second regulating valve 11 in turn, and then enters the two chromatography columns 1 for cleaning, thereby achieving small circulation cleaning. For example, the total cleaning flow rate required for the two chromatography columns 1 is 30T / H, and the flow rate of the cleaning liquid directly provided by the chromatography system 2 is 10T, then the flow rate of the cleaning liquid that can be provided in the small cycle is 20T, so as to achieve the preset purpose of cleaning and regenerating the chromatography columns.

[0070] If a cleaning method is adopted in which the cleaning liquid enters the chromatography column 1 from the bottom and exits from the top, the cleaning liquid enters the two chromatography columns 1 respectively through the second main line 5, the two second branch lines 6 and the second ports at the bottom of the two chromatography columns 1 in sequence, and the flow rate of the cleaning liquid allocated to the two second branch lines 6 can be obtained through the third flow meter 19 and the fourth flow meter 20, and the flow rate allocated to the two chromatography columns 1 is adjusted by the third regulating valve 17 and the fourth regulating valve 18 respectively. In the process of flowing through the two chromatography columns 1, the cleaning liquid cleans the substances remaining in the chromatography column 1, flows out from the first port of the chromatography column 1 after cleaning, and then flows back to the chromatography system 2 through the two first branch lines 4 and the first main line 3 in sequence, thereby realizing large-circulation cleaning of the parallel chromatography column system. In this process, a small circulation cleaning is formed by the flushing pipeline 7, the twin-screw pump 24 and the two chromatography columns 1, so as to achieve the purpose of high-flow rate cleaning of the chromatography column 1. The specific circulation process is: the cleaning liquid passes through the first main valve 8, the twin-screw pump 24, the second main valve 16 and the external discharge valve 26 in turn, so as to discharge the residual liquid and / or gas in the system, so that the cleaning liquid replaces the residual liquid and / or gas in the previous chromatography process in the system (because the chromatography column 1 requires that no gas can enter, problems such as dry filler glue will occur after air intake, resulting in poor column efficiency); then the external discharge valve 26 is closed, and the twin-screw pump 24 is controlled to have a direction opposite to the small circulation cleaning of the forward flow, and the cleaning liquid passes through the twin-screw pump 24, the second main valve 16, the third regulating valve 17 and the fourth regulating valve 18 in turn and enters the two chromatography columns 1 respectively, so as to achieve small circulation cleaning. Similarly, if the total cleaning flow rate required by the two chromatography columns 1 is 30T / H, and the cleaning liquid flow rate directly provided by the chromatography system 2 is 10T, then the cleaning liquid flow rate that can be provided in the small cycle is 20T, so as to achieve the preset purpose of cleaning and regenerating the chromatography columns.

[0071] The characteristics and advantages of the parallel chromatography column system of the utility model are:

[0072] The parallel chromatography column system can realize the expansion of the production capacity of the chromatography system, and can achieve the ideal material purity and the cleaning effect of the chromatography column while using a smaller amount of balancing liquid, eluent and regeneration liquid.

[0073] The above description is only an illustrative embodiment of the present invention and is not intended to limit the scope of the present invention. Any equivalent changes and modifications made by any person skilled in the art without departing from the concept and principle of the present invention shall fall within the scope of protection of the present invention.

Claims

1. A parallel chromatography column system, characterized in that: The parallel chromatography column system comprises: At least two chromatography columns, each of the chromatography columns having at least a first port located at the top thereof and a second port located at the bottom thereof, the first ports of the two chromatography columns being respectively connected to a first main pipe in an on-off manner through a first branch pipe, the first main pipe being used to be connected to a chromatography system in an on-off manner; the second ports of the two chromatography columns being respectively connected to a second main pipe in an on-off manner through a second branch pipe, the second main pipe being used to be connected to the chromatography system in an on-off manner, so that the two chromatography columns are connected to the chromatography system in parallel; A flushing pipeline is connected between the first main pipeline and the second main pipeline in an on-off manner to form a circulatable cleaning flow path between the first main pipeline and the second main pipeline.

2. The parallel chromatography column system according to claim 1, characterized in that: The flushing pipeline is provided with an exhaust valve to exhaust the residual gas in the parallel chromatography column system.

3. The parallel chromatography column system according to claim 1, characterized in that: The first main pipeline is provided with a first main valve for controlling the on-off of the first main pipeline, and the two first branch pipelines are respectively provided with a first regulating valve and a second regulating valve for controlling flow distribution.

4. The parallel chromatography column system according to claim 3, characterized in that: A first reducing pipe is disposed on the first main pipe and between the first main valve and the chromatography system. The inner diameter of the first reducing pipe gradually increases in a direction from the chromatography system to the first main valve.

5. The parallel chromatography column system according to claim 3 or 4, characterized in that: A second reducing pipe is respectively provided on the two first branch pipes and between the first main pipe and the first regulating valve and the second regulating valve. The inner diameter of the second reducing pipe gradually increases from the first main pipe to the first regulating valve or from the first main pipe to the second regulating valve.

6. The parallel chromatography column system according to claim 3, characterized in that: A first on-off valve and a second on-off valve are respectively provided on the two first branch pipelines.

7. The parallel chromatography column system according to claim 3, characterized in that: The first main pipeline is provided with a first flow meter, and at least one of the two first branch pipelines is provided with a second flow meter.

8. The parallel chromatography column system according to claim 3, characterized in that: The second main pipe is provided with a second main valve for controlling the on-off of the second main pipe, and the two second branch pipes are respectively provided with a third regulating valve and a fourth regulating valve for controlling flow distribution.

9. The parallel chromatography column system according to claim 8, characterized in that: A third reducing pipe is disposed on the second main pipe and between the second main valve and the chromatography system. The inner diameter of the third reducing pipe gradually increases from the chromatography system to the second main valve.

10. The parallel chromatography column system according to claim 8 or 9, characterized in that: A fourth reducing pipe is respectively provided on the two second branch pipes and between the second main pipe and the third regulating valve and the fourth regulating valve. The inner diameter of the fourth reducing pipe gradually increases from the second main pipe to the third regulating valve or from the second main pipe to the fourth regulating valve.

11. The parallel chromatography column system according to claim 8, characterized in that: The second main pipeline is provided with a third flow meter, and at least one of the two second branch pipelines is provided with a fourth flow meter.

12. The parallel chromatography column system according to claim 8, characterized in that: The flushing pipeline is provided with a fifth flow meter and a twin-screw pump capable of driving the liquid in the flushing pipeline to flow in both directions.

13. The parallel chromatography column system according to claim 8 or 12, characterized in that: At least a portion of the flushing line is connected between the first main valve and the second main valve.

Images