Tissue explant pretreatment mold

Abstract

The utility model provides a tissue explant pretreatment mold which solves the problems that in the prior art, the pretreatment speed of a tissue specimen prepared from a tissue explant is low, and reference cannot be provided for subsequent tissue explant positioning. The tissue explant pretreatment mold comprises a marking cover, a sample loading tube and a piston rod, the marking cover is arranged at the top of the sample loading tube, the piston rod comprises a piston, a connecting rod and a handle arranged below the bottom of the sample loading tube, the piston is arranged in the sample loading tube in a sliding mode, and the connecting rod is arranged on the piston. And the connecting rod is positioned between the piston and the handle. The tissue sample is put into the sample loading tube, other auxiliary forming reagents are added, and the whole pretreated sample is pushed out by the push rod after being formed, so that the structure is simple, the operation is quick, and the tissue can be quickly and conveniently pretreated. In addition, the tissue specimen pretreatment mold for preparing the tissue explant, provided by the utility model, can meet the pretreatment requirements of tissue blocks with different sizes.

Description

Technical Field

[0001] The utility model belongs to the technical field of molds, and in particular relates to a tissue explant pretreatment mold. Background Art

[0002] Tissue explants (PDEs) retain the structure and microenvironment of the corresponding human tissues and maintain the spatial distribution characteristics consistent with the original tissues. They are cultured in vitro and subsequently processed and tested while maintaining vitality. However, in the process of preparing explants, the size, toughness and strength of the explants bring many inconveniences to cutting. People usually use some auxiliary reagents to enhance their toughness or take other methods to fix their shape to make it easier to cut. However, after adding auxiliary reagents, the shape of the tissue specimens obtained by pretreatment is irregular, resulting in a slow pretreatment speed of tissue specimens used for PDEs preparation and failure to provide a reference for the subsequent positioning of tissue explants. Utility Model Content

[0003] The utility model provides a tissue explant pretreatment mold, aiming to solve the problems in the prior art that the pretreatment speed of tissue specimens prepared by the tissue explant system is slow and reference for subsequent tissue explant positioning cannot be provided.

[0004] In order to solve the above technical problems, the technical solution adopted by the utility model is:

[0005] A tissue explant pretreatment mold comprises: a marking cover, a sample loading tube and a piston rod, wherein the marking cover is arranged on the top of the sample loading tube, the piston rod comprises a piston, a connecting rod and a handle arranged below the bottom of the sample loading tube, the piston is slidably arranged in the sample loading tube, and the connecting rod is located between the piston and the handle.

[0006] In a preferred solution, the movable path of the piston is from the bottom of the sample loading tube to the top of the sample loading tube.

[0007] Based on the above scheme, the piston pushes the tissue gel block wrapped and shaped by the auxiliary molding reagent in the sample loading tube from the bottom of the sample loading tube to the top and takes it out.

[0008] In a preferred embodiment, a plurality of marking rods inserted into the sample loading tube are provided below the marking cover.

[0009] In a preferred solution, the marking rod is arranged in a region of the marking cover close to an edge.

[0010] Based on the above scheme, when the liquid in the sample tube forms a semisolid or gel block, the marking rod is inserted into the semisolid or gel block, the marking cover is screwed upward, the marking rod is detached, and a hole is left in the semisolid or gel block. The marking liquid is dripped to provide a reference for the subsequent positioning of the tissue explant.

[0011] Based on the above scheme, the marking rod can be inserted into the sample tube so as to leave a cylindrical hole after demoulding for dripping the marking liquid, thereby achieving accurate positioning of PDEs in the subsequent PDEs culture and detection process.

[0012] In a preferred embodiment, the diameter of the marking rod is 0.3-0.6 mm.

[0013] In a preferred embodiment, the loading tube is cylindrical and the handle is pancake-shaped.

[0014] In a preferred embodiment, the sample loading tube has a diameter of 0.2-1.0 cm and a height of 1.0-2.0 cm.

[0015] In a preferred solution, the diameter of the connecting rod is smaller than the diameter of the loading tube.

[0016] In a preferred embodiment, the sample loading tube is a light-transmitting plastic tube or a light-transmitting glass tube.

[0017] Based on the above scheme, the loading tube is transparent and translucent, which is convenient for observing the tissue block in the tube from the outside and for the UV curing light source to penetrate the tube wall, triggering the gelation of the auxiliary molding reagent and the auxiliary molding of the tissue specimen.

[0018] The beneficial effects of the utility model are:

[0019] The utility model provides a tissue explant pretreatment mold, which is used to put the tissue prepared for the tissue explant into a sample tube, add other auxiliary molding reagents, and after the tissue gel block is gelled and fixed, unscrew the marking cover, drip the marking liquid and fix it into shape, and finally use a push rod to push out the entire pretreated tissue specimen. The structure is simple and the operation is fast, and the tissue specimen prepared for the tissue explant can be pretreated quickly and conveniently. In addition, the tissue specimen pretreatment mold prepared for the tissue explant provided by the utility model can adapt to the pretreatment requirements of tissue blocks of different sizes, and can mark and locate near the tissue specimen, which is helpful for accurate positioning in the subsequent culture and detection of PDEs, and improves the accuracy of the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for use in the embodiments are briefly introduced below. It should be understood that the following drawings only show certain embodiments of the utility model and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1 The utility model is a schematic diagram of the structure of a tissue specimen pretreatment mold used in a tissue explantation system.

[0022] Figure 2 It is a structural schematic diagram of the marking cover in the utility model.

[0023] Figure 3 The utility model is a schematic cross-sectional view of the structure of a tissue specimen pretreatment mold part reserved for the tissue explantation system.

[0024] Description of the numbers in the figure:

[0025] 1-marking cover; 2-sample tube; 3-piston rod; 31-piston; 32-connecting rod; 33-handle; 4-marking rod; DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. It should be understood that the specific embodiments described herein are only used to explain the utility model and are not used to limit the utility model. Based on the embodiments of the utility model, all other embodiments obtained by those skilled in the art without creative work belong to the protection scope of the utility model.

[0027] like Figure 1-Figure 3 As shown, this embodiment provides a tissue explant pretreatment mold, including: a marking cover 1, a sample loading tube 2 and a piston rod 3, the marking cover 1 is covered on the top of the sample loading tube 2, the piston rod 3 includes a piston 31, a connecting rod 32 and a handle 33 arranged below the bottom of the sample loading tube 2, the piston 31 is slidably arranged in the sample loading tube 2, and the connecting rod 32 is located between the piston 31 and the handle 33.

[0028] The movable path of the piston 31 is from the bottom of the sample loading tube 2 to the top of the sample loading tube 2 .

[0029] Specifically, the push rod pushes the piston 31 from the bottom of the sample tube 2 to the top of the sample tube 2, and the piston 31 pushes the pretreated tissue specimen in the sample tube 2 to leave the sample tube 2, thereby obtaining a pretreated tissue specimen with a regular shape.

[0030] In addition, a plurality of marking rods inserted into the sample loading tube are arranged below the marking cover.

[0031] Specifically, the number of the marking rods 4 can be 1 or 2, etc., and a preferred solution is that the number of the marking rods 4 is 1. The marking rod 4 can be arranged in the middle area of ​​the marking cover 1 or in an area close to the edge, and a preferred solution is that the marking rod 4 is arranged in an area close to the edge of the marking cover 1.

[0032] After the pre-treated tissue specimen is demoulded, in order to facilitate the positioning of the subsequently prepared PDEs, on the basis of the above solution, the marking rod 4 is connected to the marking cover 1 .

[0033] The marking rod 4 has a diameter of 0.3-0.6 mm and a length of 0.9-1.9 cm.

[0034] Specifically, the diameter of the marking rod 4 may be 0.5 mm or the like.

[0035] In addition, the sample loading tube 2 is cylindrical, and the handle 33 is pancake-shaped.

[0036] Specifically, the shape of the sample loading tube 2 can be set to be square or cylindrical, etc., and the handle 33 can be set to be square or pancake-shaped, etc. In a preferred solution, the sample loading tube 2 is cylindrical, and the handle 33 is pancake-shaped.

[0037] In order to adapt to the pretreatment of tissues of different sizes, based on the above scheme, the diameter of the sample loading tube 2 is 0.2-1.0 cm and the height is 1.0-2.0 cm.

[0038] Specifically, the diameter of the sample loading tube 2 may be 0.5 cm, etc., and the height may be 1.5 cm, etc.

[0039] The diameter of the connecting rod 32 is smaller than the diameter of the sample loading tube 2 .

[0040] In addition, the sample loading tube 2 is a light-transmitting plastic tube or a light-transmitting glass tube.

[0041] The utility model is further explained below in conjunction with the working principle:

[0042] The tissue specimen to be processed and the reagent to be added are added to the sample tube 2, the marking cover 1 is covered, the marking rod 4 is inserted into the sample tube 2, and then the marking cover is screwed out upwards, and then the marking liquid is added to the channel formed by the withdrawal of the marking rod on the marking cover, and then refrigerated for 3-5 minutes. After the tissue specimen and the reagent are gelled and formed, the push rod is pushed to obtain the pre-treated tissue specimen after forming.

[0043] The present utility model is not limited to the above optional implementation modes. Under the premise of not conflicting with each other, the various schemes can be combined arbitrarily. Anyone can derive other forms of products under the inspiration of the present utility model. However, no matter what changes are made in their shapes or structures, all technical schemes that fall within the scope defined by the claims of the present utility model fall within the protection scope of the present utility model.

Claims

1. A tissue explant pretreatment mold, characterized in that: include: A marking cover, a sample loading tube and a piston rod, wherein the marking cover is arranged on the top of the sample loading tube, the piston rod comprises a piston, a connecting rod and a handle arranged below the bottom of the sample loading tube, the piston is slidably arranged in the sample loading tube, and the connecting rod is located between the piston and the handle.

2. A tissue explant pretreatment mold according to claim 1, characterized in that: The movable path of the piston is from the bottom of the sample loading tube to the top of the sample loading tube.

3. A tissue explant pretreatment mold according to claim 1, characterized in that: A plurality of marking rods inserted into the sample loading tube are arranged below the marking cover.

4. A tissue explant pretreatment mold according to claim 3, characterized in that: The marking rod is arranged in a region of the marking cover close to an edge.

5. A tissue explant pretreatment mold according to claim 4, characterized in that: The marking rod is cylindrical, with a diameter of 0.3-0.6 mm and a length of 0.9-1.9 cm.

6. A tissue explant pretreatment mold according to claim 1, characterized in that: The sample loading tube is cylindrical, and the handle is pancake-shaped.

7. A tissue explant pretreatment mold according to claim 1, characterized in that: The sample loading tube has a diameter of 0.2-1.0 cm and a height of 1.0-2.0 cm.

8. A tissue explant pretreatment mold according to claim 1, characterized in that: The diameter of the connecting rod is smaller than the diameter of the sample loading tube.

9. A tissue explant pretreatment mold according to claim 1, characterized in that: The sample loading tube is a light-transmitting plastic tube or a light-transmitting glass tube.

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