Blood CRP fluorescence inspection device based on STM32 processor

Abstract

The utility model relates to the technical field of blood CRP fluorescence detection devices, in particular to a blood CRP fluorescence detection device based on an STM32 processor, which comprises a detection strip, a code scanning module, a photoelectric detection module, a transmission module and a core chip, the photoelectric detection module comprises a laser and a photoelectric tube, the transmission module is placed on a table top, and a detection strip is placed above the transmission module in parallel; the irradiation direction of the laser is parallel to and opposite to the movement direction of the transmission module, and the photoelectric tube is perpendicular to the transmission module; a dichroic mirror is obliquely arranged at an included angle between the photoelectric tube and the laser; the code scanning module is arranged above the transmission module; the core chip is electrically connected with the code scanning module, the photoelectric detection module and the transmission module. The fluorescent substance replaces a radioactive substance to serve as a tracing mark, the defects that a radioimmunoassay method is poor in stability and large in marker harm are overcome by measuring the light intensity of fluorescent light excited by the fluorescent mark, and the whole device is stable in performance, low in cost, small in power consumption and convenient to popularize.

Description

Technical Field

[0001] The utility model relates to the technical field of blood CRP testing equipment, in particular to a blood CRP fluorescence testing device based on an STM32 processor. Background Art

[0002] C-reactive protein (CRP) is a protein (acute protein) that rises sharply in the plasma when the body is infected or the tissue is damaged. It activates complement and strengthens the phagocytosis of phagocytes to play a conditioning role, clearing pathogenic microorganisms that invade the body and damaged, necrotic, and apoptotic tissue cells. At present, there are four main methods used in CRP detectors on the market at home and abroad: based on colloidal gold immunochromatography, this method has low sensitivity and a small detection range, and is often used for qualitative detection; based on enzyme-linked immunosorbent assay, this method requires enzyme activity to be maintained, has high detection requirements and a long time, and is also often used for qualitative detection; based on radioimmunoassay, this method uses radioactive substances as markers, has poor stability, and has radiation risks and pollution; based on chemiluminescence, this method is quantitatively accurate, but the detection reagent is expensive. Utility Model Content

[0003] In order to overcome the existing deficiencies, the utility model provides a blood CRP fluorescence testing device based on an STM32 processor.

[0004] The technical solution adopted by the utility model to solve its technical problems is: a blood CRP fluorescence testing device based on an STM32 processor, comprising a detection strip, a code scanning module, a photoelectric detection module, a transmission module and a core chip; the photoelectric detection module comprises a laser and a photoelectric tube, the transmission module is placed on a desktop, and the detection strip is placed above it in parallel; the irradiation direction of the laser is parallel to and opposite to the movement direction of the transmission module, and the photoelectric tube is placed vertically to the transmission module; a dichroic mirror is arranged obliquely at an angle between the photoelectric tube and the laser; the code scanning module is arranged above the transmission module; the core chip is electrically connected to the code scanning module, the photoelectric detection module and the transmission module.

[0005] According to another embodiment of the utility model, a filter is provided in front of the laser, and the laser passes through the filter and is refracted by the dichroic mirror, then passes through a convex lens and a cylindrical mirror, and is perpendicular to the surface of the detection strip; the laser is electrically connected to the core chip

[0006] According to another embodiment of the utility model, it further includes that a convex lens 2 and a filter are arranged in sequence from top to bottom directly below the photoelectric tube, and are electrically connected to the core chip; the photoelectric tube, the convex lens 2 and the filter are perpendicular to the movement direction of the transmission module, and are arranged above the color separation mirror.

[0007] According to another embodiment of the utility model, it further includes that the core chip selects a STM32 series processor, receives information transmitted by a photoelectric tube, a code scanning module, and an external keyboard, outputs information to a display screen, an EEPROM card, an SD card, a transmission module and a laser, and communicates bidirectionally with a client via Bluetooth.

[0008] According to another embodiment of the utility model, the transmission module further includes a motor drive circuit and a stepper motor, the motor drive circuit adopts a high-segmentation chip A3967, receives control signals from the core chip through pins Motor-STEP, Motor-DIR, and Motor-MEN, transmits the A3967 chip through three optical couplers, and is connected to the stepper motor through OUT1A, OUT2A, OUT1B, and OUT2B.

[0009] According to another embodiment of the utility model, the photoelectric tube collects the signal, amplifies the signal, filters it, and rectifies it through the conditioning circuit, and then samples the data through the AD module of the sampling circuit and sends it to the core chip.

[0010] According to another embodiment of the utility model, the code scanning module further includes a USB code scanning device, which is arranged just above the detection bar on the transmission module.

[0011] According to another embodiment of the utility model, the test strip further includes a shell and a test paper, the shell is provided with a sample drop hole, a scanning frame and a barcode; the test paper is provided with a sample pad just below the sample drop hole, and the bottom is connected to the analysis membrane through a binding pad; the analysis membrane is provided with a substrate at the bottom and a water-absorbing pad at the other side, which is arranged just below the scanning frame; the analysis membrane is provided with a test band T and a control band C.

[0012] The beneficial effects of the utility model are that fluorescent substances are used instead of radioactive substances as tracer markers, and the fluorescence intensity emitted by the fluorescent markers is measured to overcome the shortcomings of the radioimmunoassay method, such as poor stability and great harm of the markers. The detection has good stability and high accuracy, and meets the requirements of clinical detection. The utility model is equipped with a client and can be used for home medical treatment, so that users can view and manage personal detection records conveniently. The whole device has stable performance, low cost, low power consumption, and is easy to promote. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] The utility model is further described below in conjunction with the accompanying drawings and embodiments.

[0014] Figure 1 It is a structural schematic diagram of the utility model;

[0015] Figure 2 is a schematic diagram of the structure of the test strip;

[0016] Figure 3It is the circuit diagram of the motor drive circuit;

[0017] Figure 4 It is an operation flow chart of the utility model.

[0018] In the figure, 1, detection strip, 2, code scanning module, 3, photoelectric detection module, 4, transmission module, 5, core chip, 6, laser, 7, photoelectric tube, 8, color separation mirror, 9, filter, 10, convex lens 1, 11, cylindrical mirror, 12, convex lens 2, 13, filter, 14, motor drive circuit, 15, stepper motor, 16, conditioning circuit, 17, sampling circuit, 18, shell, 19, test paper, 20, sample drop hole, 21, scanning frame, 22, barcode. DETAILED DESCRIPTION

[0019] like Figure 1 It is a structural schematic diagram of the utility model, a blood CRP fluorescence test device based on the STM32 processor, including a test strip 1, a code scanning module 2, a photoelectric detection module 3, a transmission module 4 and a core chip 5; the photoelectric detection module 3 includes a laser 6 and a photoelectric tube 7, the transmission module 4 is placed on the desktop, and the test strip 1 is placed above it in parallel; the irradiation direction of the laser 6 is parallel to and opposite to the movement direction of the transmission module 4, and the photoelectric tube 7 is placed vertically to the transmission module 4; the angle between the photoelectric tube 7 and the laser 6 is provided with a dichroic mirror 8 inclined; the code scanning module 2 is arranged above the transmission module 4; the core chip 5 is electrically connected to the code scanning module 2, the photoelectric detection module 3, and the transmission module 4.

[0020] According to another embodiment of the utility model, a filter 9 is provided in front of the laser 6, and the laser passes through the filter 9 and is refracted by the dichroic mirror 8, passes through the convex lens 10 and the cylindrical mirror 11, and is perpendicular to the surface of the detection strip 1; the laser 6 is electrically connected to the core chip 5

[0021] According to another embodiment of the utility model, it further includes that a convex lens 12 and a filter 13 are arranged in sequence from top to bottom directly below the photoelectric tube 7, and are electrically connected to the core chip 5; the photoelectric tube 7, the convex lens 12 and the filter 13 are perpendicular to the movement direction of the transmission module 4, and are arranged above the color separation mirror 8.

[0022] Specifically, the laser 6 emits ultraviolet light with a central wavelength of 340 nm, which is focused by the convex lens 10 after being filtered by the 320-360 nm bandpass filter 9 and reflected by the dichroic mirror 8, and is projected onto the detection strip through the cylindrical mirror 11 to form a line spot. The stepper motor 15 drives the detection strip 1 to move forward slowly, and the fluorescent markers near the test strip T and the control strip C are excited to emit fluorescence. The fluorescence is emitted in parallel through the convex lens 10, and after being transmitted by the dichroic mirror 8 and filtered out by the 600-620 nm bandpass filter 13, it is focused by the convex lens 2 12 onto the receiving surface of the photoelectric tube 7 and converted into an electrical signal, thereby obtaining the sample fluorescence curve.

[0023] According to another embodiment of the utility model, it further includes that the core chip 5 selects a STM32 series processor, receives information transmitted by the photoelectric tube 7, the code scanning module 2, and the external keyboard, outputs information to the display screen, EEPROM card, SD card, transmission module 4 and laser 6, and communicates with the client bidirectionally via Bluetooth.

[0024] According to another embodiment of the utility model, the transmission module 4 further includes a motor drive circuit 14 and a stepper motor 15, the motor drive circuit 14 adopts a high-segmentation chip A3967, receives control signals of the core chip 5 through pins Motor-STEP, Motor-DIR, and Motor-MEN, transmits the A3967 chip through three optical couplers, and is connected to the stepper motor 15 through OUT1A, OUT2A, OUT1B, and OUT2B.

[0025] According to another embodiment of the utility model, the photoelectric tube 7 collects the signal, and then the signal is amplified, filtered and rectified by the conditioning circuit 16, and then the data is sampled by the AD module of the sampling circuit 17 and sent to the core chip 5. The active filter of the conditioning circuit 16 has a relatively ideal input and output impedance, does not require impedance matching, can be directly cascaded, and the circuit gain compensation of different frequencies can be performed by adjusting the bridge arm resistance. In order to ensure signal stability, the system also performs precision rectification after filtering to ensure the accuracy of the sampled data.

[0026] According to another embodiment of the present invention, the code scanning module 2 further includes a USB code scanning device, which is arranged directly above the detection strip 1 on the transmission module 4.

[0027] According to another embodiment of the utility model, the test strip 1 further includes a shell 18 and a test paper 19, the shell 18 is provided with a sample drop hole 20, a scanning frame 21 and a barcode 22; the test paper 19 is provided with a sample pad just below the sample drop hole 20, and the bottom is connected to the analysis membrane through a binding pad; the analysis membrane is provided with a substrate at the bottom arranged just below the scanning frame 21, and a water absorbent pad is provided on the other side; the analysis membrane is provided with a test band T and a control band C.

[0028] Specifically, the CRP fluorescence detection immunoassay utilizes the specificity of the immune response, combined with chromatography technology and fluorescence tracing technology, to achieve quantitative detection of CRP concentration. The CRP antibody corresponding to the target CRP antigen is fluorescently labeled. According to the specificity of the immune response, the target CRP antigen reacts with the fluorescent antibody to form a fluorescent complex. The complex sample is dropped into the sample drop hole 20 of the test strip 1. After the test paper analysis membrane chromatography, the fluorescent complex is separated at the test band T, and the free fluorescent antibody is at the control band C. The specific wavelength excitation light scans the test strip 1, and the fluorescent marker is excited to fluoresce. The higher the CRP antigen concentration in the blood sample, the stronger the fluorescence excited at the T band and the larger the peak value.

[0029] In the specific operation process, the barcode scanning module 2 scans the barcode 22 of the detection strip 1, so that the core chip can read the barcode ID information in the EEPROM card and process the sampling data; the photoelectric detection module 3 includes a laser 6 and a phototube 7, which scan the detection strip 1 and convert the excited fluorescence into an electrical signal; the transmission module 4 includes a motor drive circuit 14 and a stepper motor 15, which drive the detection strip 1 to move and complete the fluorescence scanning; the electrical signal collected by the phototube 7 is amplified, filtered and rectified by the conditioning circuit 16, and is provided for sampling data by the sampling circuit 17; the core chip 5 processes the sampling data and outputs the results to the display screen, or transmits them to the client via Bluetooth; the client has its own database to realize functions such as user login, analysis and display, and record saving.

[0030] The above description is only illustrative rather than restrictive of the present invention. Those skilled in the art will understand that many modifications, changes or equivalents may be made without departing from the spirit and scope defined by the appended claims, but all will fall within the scope of protection of the present invention.

Claims

1. A blood CRP fluorescence testing device based on STM32 processor, characterized in that: The invention comprises a detection strip (1), a code scanning module (2), a photoelectric detection module (3), a transmission module (4) and a core chip (5); the photoelectric detection module (3) comprises a laser (6) and a photoelectric tube (7); the transmission module (4) is placed on a table, and the detection strip (1) is placed above the table in parallel; the irradiation direction of the laser (6) is parallel to and opposite to the movement direction of the transmission module (4); the photoelectric tube (7) and the transmission module (4) are placed vertically; a dichroic mirror (8) is arranged at an angle between the photoelectric tube (7) and the laser (6); the code scanning module (2) is arranged above the transmission module (4); and the core chip (5) is electrically connected to the code scanning module (2), the photoelectric detection module (3) and the transmission module (4).

2. The blood CRP fluorescence testing device based on the STM32 processor according to claim 1 is characterized in that: A filter (9) is provided directly in front of the laser (6); the laser light passes through the filter (9), is refracted by the dichroic mirror (8), passes through a convex lens 1 (10) and a cylindrical mirror (11), and is perpendicular to the surface of the detection strip (1); the laser (6) is electrically connected to the core chip (5).

3. The blood CRP fluorescence testing device based on the STM32 processor according to claim 1 is characterized in that: A convex lens 2 (12) and a filter (13) are arranged in order from top to bottom directly below the photoelectric tube (7), and are electrically connected to the core chip (5); the photoelectric tube (7), the convex lens 2 (12) and the filter (13) are perpendicular to the movement direction of the transmission module (4), and are arranged above the dichroic mirror (8).

4. The blood CRP fluorescence testing device based on the STM32 processor according to claim 1 is characterized in that: The core chip (5) uses a processor of the STM32 series, receives information transmitted by a photoelectric tube (7), a code scanning module (2), and an external keyboard, outputs the information to a display screen, an EEPROM card, an SD card, a transmission module (4), and a laser (6), and communicates bidirectionally with a client via Bluetooth.

5. The blood CRP fluorescence testing device based on the STM32 processor according to claim 4 is characterized in that: The transmission module (4) comprises a motor drive circuit (14) and a stepper motor (15). The motor drive circuit (14) adopts a high-resolution chip A3967, receives control signals from a core chip (5) via pins Motor-STEP, Motor-DIR, and Motor-MEN, transmits the signals to the A3967 chip via three optical couplers, and is connected to the stepper motor (15) via OUT1A, OUT2A, OUT1B, and OUT2B.

6. The blood CRP fluorescence testing device based on the STM32 processor according to claim 4 is characterized in that: The photoelectric tube (7) collects the signal, which is amplified, filtered and rectified by the conditioning circuit (16), and then the data is sampled by the AD module of the sampling circuit (17) and sent to the core chip (5).

7. The blood CRP fluorescence testing device based on the STM32 processor according to claim 4 is characterized in that: The code scanning module (2) comprises a USB code scanning device, which is arranged directly above the detection bar (1) on the transmission module (4).

8. The blood CRP fluorescence testing device based on the STM32 processor according to claim 1 is characterized in that: The test strip (1) comprises a shell (18) and a test paper (19); the shell (18) is provided with a sample drop hole (20), a scanning frame (21) and a barcode (22); the test paper (19) is provided with a sample pad directly below the sample drop hole (20), and the bottom is connected to the analysis membrane via a binding pad; the analysis membrane is provided with a substrate directly below the scanning frame (21), the bottom is provided with a water absorption pad, and the other side is provided with a test band T and a control band C.

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