Denitrification microbial inoculum enrichment culture device

Abstract

The utility model relates to the technical field of strain culture, and discloses a denitrification fungicide enrichment culture device which comprises a box body and a plurality of culture boxes movably installed on the box body, a liquid containing box used for storing liquid is installed on the upper portion of the box body, a first liquid storage cavity and a second liquid storage cavity are formed in the liquid containing box, and the first liquid storage cavity and the second liquid storage cavity are communicated with each other. The lower portion of the first liquid storage cavity is communicated with the lower portion of the second liquid storage cavity, a liquid spraying opening communicated with the interior of the second liquid storage cavity is formed in the upper portion of the liquid containing box, a pressing plate which is pressed downwards is installed in the first liquid storage cavity, and the first liquid storage cavity and the second liquid storage cavity which are communicated with each other are formed in the liquid containing box; the nutrient solution in the first liquid storage cavity can flow into the second liquid storage cavity under the extrusion force, the liquid level is continuously increased along with the continuous increase of the nutrient solution in the second liquid storage cavity, when the liquid level in the second liquid storage cavity is higher than the liquid spraying opening, the nutrient solution flows into the multiple incubators, and the nutrient solution can be added into the multiple incubators at the same time.

Description

Technical Field

[0001] The utility model relates to the technical field of bacterial culture, in particular to a denitrification bacterial agent enrichment and culture device. Background Art

[0002] Biological denitrification refers to the process in which organic nitrogen and ammonia nitrogen in sewage are converted into nitrogen gas through ammoniation, nitrification and denitrification under the combined action of microorganisms. It is economical, effective, easy to operate and has no secondary pollution. It is recognized as a promising method, and new results are constantly reported on technical research in this area.

[0003] During the cultivation process of denitrifying bacteria agents on the market, several denitrifying bacteria agents are cultivated simultaneously in the culture boxes each time. If it is necessary to add nutrient solution to the culture boxes, the nutrient solution needs to be added to the culture boxes one by one, which results in low efficiency and high labor workload.

[0004] Therefore, we proposed a denitrifying bacteria enrichment culture device to solve the above problems. Utility Model Content

[0005] The purpose of the utility model is to solve the above-mentioned technical problems.

[0006] To achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a denitrifying bacteria agent enrichment and cultivation device, comprising a box body and a plurality of cultivation boxes movably installed on the box body, a liquid storage box for storing liquid is installed on the upper part of the box body, a first liquid storage chamber and a second liquid storage chamber are opened inside the liquid storage box, and the lower part of the first liquid storage chamber is communicated with the lower part of the second liquid storage chamber, a liquid spraying port communicated with the second liquid storage chamber is installed on the upper part of the liquid storage box, a downward pressing plate is installed inside the first liquid storage chamber, and a pressing rod extending to the outside of the box body is installed on the upper part of the pressing plate; when the pressing plate moves downward, the liquid in the first liquid storage chamber flows into the second liquid storage chamber under the condition of being squeezed, and as the liquid in the second liquid storage chamber increases, the liquid level also continues to rise. When the liquid level in the second liquid storage chamber is higher than the position of the liquid spraying port, the liquid in the second liquid storage chamber flows into the cultivation box through the plurality of liquid spraying ports.

[0007] Furthermore, a first partition is provided between the first liquid storage cavity and the second liquid storage cavity, the top of the first partition is fixedly connected to the inner top wall of the liquid storage tank, and a certain gap is left between the bottom of the first partition and the inner bottom wall of the liquid storage tank.

[0008] Furthermore; a second partition is provided between the first liquid storage chamber and the second liquid storage chamber, the top and bottom of the second partition are respectively fixedly connected to the inner top wall and the inner bottom wall of the first liquid storage chamber, and a plurality of water flow holes are opened at the lower part of the second partition.

[0009] Furthermore, a slide cylinder is fixed on the top of the box body, and the upper part of the pressure rod slides downward and is installed inside the slide cylinder.

[0010] Furthermore; a limit plate is fixed on the pressure rod located in the slide cylinder, a limit groove is provided on the circumferential surface of the limit plate, and a spring and a steel ball slidably installed in the limit groove are fixed inside the limit groove, and one end of the spring is in conflict with the outer surface of the steel ball; the inner side wall of the slide cylinder is provided with multiple rows of clamping holes along the axial direction, the spacing on each row of clamping holes is different, and the uppermost clamping holes in the multiple rows are located on a horizontal plane.

[0011] Furthermore, the inner wall of the slide cylinder is also provided with a slide groove, and the multiple card holes in each column are located in the same slide groove. The outer surface of the limit plate is fixed with a limit block, and the limit block is slidably installed inside the limit groove.

[0012] Furthermore, an annular groove is provided on the inner side wall of the upper part of the slide cylinder along the circumferential direction, and the upper parts of the plurality of slide grooves are connected to the annular groove.

[0013] Furthermore, the outer surface of the pressure rod is engraved with marking lines corresponding to the steel balls along the axial direction, and the top of the slide cylinder is marked with a plurality of marks along the circumferential direction, and each mark is located directly above each column of the card holes.

[0014] Furthermore, a measuring cylinder is installed on the upper part of the box body, and a measuring rod is installed inside the measuring cylinder for sliding up and down. The bottom end of the measuring rod extends to the inside of the second liquid storage chamber and a float is fixed thereon. The top end of the measuring rod passes through the measuring cylinder and a baffle is fixed thereon.

[0015] Furthermore, an air vent connected to the first liquid storage chamber is provided on the upper portion of the box body.

[0016] Beneficial effects of the utility model:

[0017] The utility model has a pressure plate inside the liquid storage tank and a pressure rod fixed on the top of the pressure plate, and when the pressure rod drives the pressure plate downward to apply pressure, the nutrient solution in the liquid storage tank can be squeezed; a first liquid storage chamber and a second liquid storage chamber that are interconnected are provided inside the liquid storage tank, and when the nutrient solution in the first liquid storage chamber is subjected to the squeezing force, the nutrient solution can flow into the second liquid storage chamber, and as the nutrient solution in the second liquid storage chamber continues to increase, the liquid level also continues to rise, and when the liquid level in the second liquid storage chamber is higher than the liquid spray port and flows into a plurality of culture boxes, nutrient solution can be added to a plurality of culture boxes at the same time, thereby improving work efficiency.

[0018] The utility model is provided with a spring and a steel ball on the limit plate and a plurality of clamping holes are provided on the inner side wall of the slide cylinder along the axial direction, so that the pressure plate can also drive the limit plate to move downward in the process of driving the pressure rod to move downward, and then the limit plate drives the steel ball to move downward through the spring, so that when the steel ball is disengaged from one of the clamping holes, it moves downward and is clamped in the inside of the next clamping hole, thereby playing the role of limiting the pressure rod, so that the pressure rod can be limited after moving downward to a specified distance each time, thereby being able to control the downward movement distance of the pressure rod, so that the nutrient solution in the liquid tank can only be put into the liquid tank in a specified amount each time, thereby reducing the phenomenon of excessive or insufficient nutrient solution being put into.

[0019] The utility model has multiple rows of clamping holes with different spacings on the inner side wall of the pressure plate along the circumferential direction, so that the pressure rod can drive the limit plate to rotate, and the limit plate drives the steel balls to be clamped on the clamping holes in different rows through the spring, so that the pressure rod can be adjusted and limited when moving downward to different distances, and the amount of nutrient solution added can be adjusted. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a three-dimensional structural schematic diagram of the utility model;

[0021] Figure 2 It is a schematic diagram of the internal structure of the utility model;

[0022] Figure 3 It is a schematic diagram of the cross-sectional structure of the box body in the utility model;

[0023] Figure 4 It is a schematic diagram of the cross-sectional structure of the liquid-containing tank in the utility model;

[0024] Figure 5 It is a schematic diagram of the internal structure of the slide cylinder in the utility model;

[0025] Figure 6 It is a schematic diagram of the cross-sectional structure of the slide cylinder in the utility model;

[0026] Figure 7 It is a schematic diagram of the cross-sectional structure of the limiting plate in the utility model;

[0027] Figure 8 This is a schematic diagram of the cross-sectional structure of the measuring tube in the utility model.

[0028] The names corresponding to the marks in the figure are:

[0029] 1. Box body; 2. Incubator; 3. Liquid storage box; 31. First liquid storage chamber; 32. Second liquid storage chamber; 4. Pressing plate; 5. Pressing rod; 61. First partition; 62. Second partition; 621. Water flow hole; 7. Slide cylinder; 8. Limiting plate; 91. Spring; 92. Steel ball; 10. Clamping hole; 11. Slide groove; 12. Limiting block; 13. Annular groove; 14. Marking line; 15. Marking; 16. Measuring cylinder; 17. Measuring rod; 18. Floating ball; 19. Baffle; 20. Air vent; 21. Placement hole; 22. Slide rail; 23. Liquid inlet; 24. Extension tube; 25. Pressing handle. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field belong to the scope of protection of the utility model.

[0031] Embodiment 1 of the present utility model:

[0032] A denitrifying bacteria enrichment and culture device comprises a "cylindrical" box body 1; a plurality of placement holes 21 are equidistantly provided on the circumferential surface of the box body 1, and a culture box 2 can be placed in the box body 1 through the placement holes 21 by means of the placement holes 21; a slide rail 22 is symmetrically provided on the inner side wall of the box body 1 about the placement holes 21, so that the culture box 2 can slide smoothly in the box body 1; the interior of the culture box 2 is used for storing denitrifying bacteria mud; thereby, the denitrifying bacteria agent can be cultured.

[0033] A liquid storage tank 3 for storing nutrient solution is fixed at the center of the top wall of the box body 1, and a liquid inlet 23 connected to the liquid storage tank 3 is installed on the upper part of the box body 1. Through the setting of the liquid inlet 23, nutrient solution can be added to the liquid storage tank 3, and a sealing cover is threadedly installed at the opening of the liquid inlet 23. Through the setting of the sealing cover, a sealing effect is achieved. A first liquid storage chamber 31 and a second liquid storage chamber 32 are sequentially arranged in the liquid storage tank 3 from the inside to the outside, and the liquid inlet 23 is connected with the upper part of the second liquid storage chamber 32. The first liquid storage chamber 31 has a "cylindrical" structure, and the second liquid storage chamber 32 is set to a "ring" shape. It should be noted that: the lower part of the first liquid storage chamber 31 is connected with the lower part of the second liquid storage chamber 32, and a liquid spray port corresponding to the incubator 2 is installed on the circumferential surface of the upper part of the liquid storage tank 3. In order to lengthen the liquid spray port The length of the bottle can be adjusted by the pressure relief device 21, and the pressure relief device 22 can be installed at the position of the bottle nozzle 22 to prevent the nutrient solution in the second liquid storage chamber 32 from flowing into the incubator 2 more smoothly and avoid spilling outside the incubator 2. A pressure plate 4 is installed inside the first liquid storage chamber 31 for sliding up and down. In order to increase the sealing performance, a sealing plug (not shown in the figure) can be fixed on the circumferential surface of the pressure plate 4. When the pressure plate 4 moves downward, the nutrient solution can be prevented from penetrating above the pressure plate 4. At the same time, an air hole 20 connected to the first liquid storage chamber 31 is also opened in the upper part of the box body 1. Through the setting of the air hole 20, when the pressure plate 4 moves upward, the gas in the first liquid storage chamber 31 can be discharged. A pressure rod 5 is fixed at the center of the top of the pressure plate 4, and the top of the pressure rod 5 passes through the box body 1 to fix the hand pressing handle 25, so as to facilitate pressing the pressure rod 5 downward.

[0034] There are two ways to connect the lower part of the first liquid storage chamber 31 with the lower part of the second liquid storage chamber 32. This embodiment can adopt any of the following ways:

[0035] The first connection mode: the interior of the liquid storage tank 3 is divided into a first liquid storage chamber 31 and a second liquid storage chamber 32 by a first partition plate 61, the top of the first partition plate 61 is fixedly connected to the inner top wall of the liquid storage tank 3, and a certain gap is left between the bottom of the first partition plate 61 and the inner bottom wall of the liquid storage tank 3.

[0036] The second connection mode: the interior of the liquid storage tank 3 is divided into a first liquid storage chamber 31 and a second liquid storage chamber 32 by a second partition plate 62, the top and bottom of the second partition plate 62 are respectively fixedly connected to the inner top wall and the inner bottom wall of the first liquid storage chamber 31, and a plurality of water flow holes 621 are opened at the lower part of the second partition plate 62.

[0037] When cultivating the denitrifying bacteria agent, firstly, the denitrifying bacteria mud is placed in a plurality of culture boxes 2 respectively, and then the plurality of culture boxes 2 are closed with the box body 1. When it is necessary to add nutrient solution to the culture box 2, firstly, the pressing handle 25 is pressed downward, and the pressing handle 25 drives the pressing plate 4 to move downward in the first liquid storage chamber 31 through the pressing rod 5. When the nutrient solution in the first liquid storage chamber 31 is squeezed, it flows into the second liquid storage chamber 32. As the nutrient solution in the second liquid storage chamber 32 continues to increase, the liquid level also continues to rise. If the liquid level in the second liquid storage chamber 32 is higher than the liquid spraying port, the nutrient solution in the second liquid storage chamber 32 flows to the inside of the culture box 2 respectively through the plurality of liquid spraying ports, and the nutrient solution can be placed in a plurality of culture boxes 2 at the same time, thereby improving the nutrient solution placement efficiency.

[0038] Embodiment 2

[0039] Although the above-mentioned embodiment 1 can simultaneously release nutrient solution into multiple culture boxes 2, it is difficult to control the amount of release during the release process, and it is easy to release too much or too little. Therefore, in order to solve the above-mentioned technical problems, improvements are made on the basis of embodiment 1.

[0040] A slide 7 is vertically fixed at the center of the top of the box body 1, and the upper part of the pressure rod 5 is slidably installed inside the slide 7 up and down. A limit plate 8 is fixed on the pressure rod 5, and the limit plate 8 is located inside the slide 7. A limit block 12 is fixed on the outer surface of the limit plate 8. A plurality of slide grooves 11 are provided on the inner side wall of the slide 7 in the circumferential direction. The limit block 12 on the limit plate 8 can only slide up and down in one slide groove 11 at a time. An annular groove 13 is provided on the inner side wall of the upper part of the slide 7, and the upper parts of the plurality of slide grooves 11 are connected with the annular groove 13. Through the setting of the annular groove 13, when the limit plate 8 moves upward to the inside of the annular groove 13, it can be rotated and adjusted, so that the limit blocks 12 on the limit plate 8 can be adjusted to the remaining slide grooves 11 to slide up and down.

[0041] A plurality of latching holes 10 are equidistantly formed on the side wall of each slide groove 11 . It should be noted that the spacing between the latching holes 10 in each row is different, and the latching holes 10 at the top of each row are located on the same horizontal plane and inside the annular groove 13 .

[0042] A limiting groove is provided on the limiting block 12, and a spring 91 and a steel ball 92 slidably installed in the limiting groove are fixed inside the limiting groove, and one end of the spring 91 is in conflict with the outer surface of the steel ball 92; part of the outer surface of the steel ball 92 extends to the outside of the limiting groove, but the steel ball 92 cannot be taken out of the limiting groove, so that the steel ball 92 can be clamped in one of the clamping holes 10.

[0043] A measuring cylinder 16 is installed on the upper part of the box body 1, and a measuring rod 17 is installed inside the measuring cylinder 16 so as to slide up and down. The measuring rod 17 is marked with scale values. The bottom end of the measuring rod 17 extends to the inside of the second liquid storage chamber 32 and a float 18 is fixed thereto, so that the float 18 can float on the liquid level of the nutrient solution. A baffle 19 is fixed to the top of the measuring rod 17 through the measuring cylinder 16. The baffle 19 is set to limit the measuring rod 17 and prevent the measuring rod 17 from falling into the liquid tank 3. By setting the measuring cylinder 16, the measuring rod 17, the float 18 and the baffle 19, when the nutrient solution is added into the liquid tank 3, the amount of the added nutrient solution can be observed.

[0044] The outer surface of the pressure rod 5 is engraved with a marking line 14 corresponding to the steel ball 92 along the axial direction, and the top of the slide cylinder 7 is marked with a plurality of marks 15 along the circumferential direction, and each mark 15 is located directly above each column of the card holes 10. The setting of the marking line 14 and the mark 15 plays a marking role.

[0045] When the solution is quantitatively added into the incubator 2, the pressure rod 5 is first pressed downward. The pressure rod 5 drives the pressure plate 4 to press downward, and can also drive the limit plate 8 to move downward. When the limit plate 8 drives the steel ball 92 to move downward through the spring 91, the spherical surface of the steel ball 92 at the edge of one of the clamping holes 10 is squeezed. Under the extrusion force, the steel ball 92 moves into the limiting groove and squeezes the spring 91. At this time, the spring 91 changes from the original state to the compressed state, so that the steel ball 92 slowly moves out from the inside of the clamping hole 10. If the limit plate 8 drives the steel ball 92 to move downward to a specified distance, the steel ball 92 is compared with the next clamping hole 10, and the spring 91 changes from the compressed state to the original state and pushes the steel ball 92, so that the steel ball 92 is clamped into the clamping hole 10 again, thereby playing a limiting role, so that the pressure rod 5 can only move downward by a specified distance each time, and then the pressure plate 4 presses the nutrient solution in the first liquid storage chamber 31, and then the nutrient solution is put into the incubator 2 in the same way as above.

[0046] If too much or too little nutrient solution is added to the incubator 2 each time and adjustment is required, first pull the pressure rod 5 upwards. The pressure rod 5 drives the pressure plate 4 to move upwards and can also drive the limit plate 8 to move upwards, so that the limit block 12 on the circumferential surface of the limit plate 8 moves to the inside of the annular groove 13 and is engaged with the uppermost clamping hole 10. Then, the pressure rod 5 is rotated again, so that the pressure rod 5 drives the limit block 12 to rotate through the limit plate 8, so that the steel ball 92 moves out of the clamping hole 10 and rotates into the clamping holes 10 on the remaining rows. Then, the pressure rod 5 is pressed downwards again to adjust the distance the pressure rod 5 moves downwards, so that the distance the pressure rod 5 moves downwards on the pressure plate 4 will also be adjusted, thereby adjusting the amount of nutrient solution added.

Claims

1. A denitrifying bacteria enrichment and culture device, comprising a housing (1) and a plurality of culture boxes (2) movably mounted on the housing (1), characterized in that: A liquid storage box (3) for storing liquid is installed on the upper part of the box body (1), a first liquid storage chamber (31) and a second liquid storage chamber (32) are provided inside the liquid storage chamber (3), and the lower part of the first liquid storage chamber (31) is connected with the lower part of the second liquid storage chamber (32), a liquid spraying port connected with the second liquid storage chamber (32) is installed on the upper part of the liquid storage chamber (3), a downward pressing plate (4) is installed inside the first liquid storage chamber (31), and a pressing rod (5) extending to the outside of the box body (1) is installed on the upper part of the pressing plate (4); when the pressing plate (4) moves downward, the liquid in the first liquid storage chamber (31) is squeezed and flows into the second liquid storage chamber (32), and as the liquid in the second liquid storage chamber (32) increases, the liquid level also continues to rise. When the liquid level in the second liquid storage chamber (32) is higher than the position of the liquid spraying port, the liquid in the second liquid storage chamber (32) flows into the incubator (2) through the plurality of liquid spraying ports.

2. A denitrifying bacteria agent enrichment and cultivation device according to claim 1, characterized in that: A first partition (61) is provided between the first liquid storage chamber (31) and the second liquid storage chamber (32); the top of the first partition (61) is fixedly connected to the inner top wall of the liquid storage tank (3); and a certain gap is left between the bottom of the first partition (61) and the inner bottom wall of the liquid storage tank (3).

3. A denitrifying bacteria agent enrichment and cultivation device according to claim 1, characterized in that: A second partition (62) is provided between the first liquid storage chamber (31) and the second liquid storage chamber (32); the top and bottom of the second partition (62) are respectively fixedly connected to the inner top wall and the inner bottom wall of the first liquid storage chamber (31); and a plurality of water flow holes (621) are provided at the lower part of the second partition (62).

4. A denitrifying bacteria agent enrichment and cultivation device according to claim 1, characterized in that: A slide cylinder (7) is fixed on the top of the box body (1), and the upper part of the pressure rod (5) is slid downward and installed inside the slide cylinder (7).

5. A denitrifying bacteria agent enrichment and cultivation device according to claim 1, characterized in that: A limit plate (8) is fixed on a pressure rod (5) located in the slide cylinder (7), and a limit groove is provided on the circumferential surface of the limit plate (8), and a spring (91) and a steel ball (92) slidably installed in the limit groove are fixed inside the limit groove, and one end of the spring (91) is in contact with the outer surface of the steel ball (92), and the inner side wall of the slide cylinder (7) is provided with multiple rows of clamping holes (10) along the axial direction, and the spacing between the clamping holes (10) in each row is different, and the uppermost clamping holes (10) in the multiple rows are located on the same horizontal plane.

6. A denitrifying bacteria enrichment and cultivation device according to claim 5, characterized in that: The inner wall of the slide cylinder (7) is also provided with a slide groove (11), and the plurality of clamping holes (10) in each row are located in the same slide groove (11). A limit block (12) is fixed on the outer surface of the limit plate (8), and the limit block (12) is slidably installed inside the slide groove (11) up and down.

7. A denitrifying bacteria enrichment and cultivation device according to claim 6, characterized in that: An annular groove (13) is provided on the inner side wall of the upper part of the slide cylinder (7) along the circumferential direction, and the upper parts of the plurality of slide grooves (11) are connected to the annular groove (13).

8. A denitrifying bacteria agent enrichment and cultivation device according to any one of claims 5 to 7, characterized in that: The outer surface of the pressure rod (5) is engraved with a marking line (14) corresponding to the steel ball (92) along the axial direction, and the top of the slide cylinder (7) is marked with a plurality of marks (15) along the circumferential direction, and each mark (15) is located directly above each column of the clamping holes (10).

9. A denitrifying bacteria agent enrichment and cultivation device according to claim 1, characterized in that: A measuring cylinder (16) is installed on the upper part of the box body (1), and a measuring rod (17) is installed inside the measuring cylinder (16) so as to slide up and down. The bottom end of the measuring rod (17) extends to the inside of the second liquid storage chamber (32) and is fixed with a floating ball (18). The top end of the measuring rod (17) passes through the measuring cylinder (16) and is fixed with a baffle (19).

10. The denitrifying bacteria agent enrichment and cultivation device according to claim 1, characterized in that: The upper portion of the box body (1) is provided with an air hole (20) which is in communication with the first liquid storage chamber (31).

Images