Optical detection system for nucleic acid detection equipment and nucleic acid detection equipment
By setting detection channel components side by side in the optical detection system of the nucleic acid detection equipment, the scanning stroke is shortened, and the complex structure and assembly difficulties in the process of miniaturization of the equipment are solved, and the effective miniaturization design of the equipment is realized.
Patent Information
- Application Number
- CN202420646462.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-03-29
- Publication Date
- 2024-12-20
- Estimated Expiration
- 2034-03-29
AI Technical Summary
During the miniaturization process, existing multi-channel and multi-sample nucleic acid detection equipment encounters problems such as complex structure, difficulty in assembly and excessive scanning stroke of the optical detection system, making it difficult to achieve effective miniaturization.
By setting the first detection channel assembly and the second detection channel assembly side by side in the optical detection system, the scanning stroke is shortened, and the optical detection module is moved longitudinally by using a movable mount and a transmissive member, thereby reducing the longitudinal length of the optical detection system, which is suitable for Miniaturized design of nucleic acid detection equipment.
The space layout of the optical detection system is easy to miniaturize, reduces the overall width and longitudinal motion stroke of the equipment, simplifies the assembly process, and meets the miniaturization needs of nucleic acid detection equipment.
Smart Images
Figure CN222205243U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of nucleic acid detection, and specifically relates to an optical detection system for nucleic acid detection equipment and nucleic acid detection equipment. Background Art
[0002] PCR technology is a molecular biology technology that amplifies specific DNA fragments in vitro. Its biggest feature is that it can greatly increase trace amounts of DNA. PCR technology has the characteristics of strong specificity, high sensitivity, low purity requirements, simplicity and rapidity, and is therefore widely used in molecular biology detection and analysis. Nowadays, multi-channel and multi-sample nucleic acid detection equipment (such as PCR instruments) and their optical detection systems are developing in the direction of miniaturization.
[0003] The existing technology uses turntable scanning to achieve scanning, where the turntable is equipped with excitation and emission of multiple channels. To ensure the rotation and scanning of the turntable, the motor is relatively large, and a conductive slip ring is used, and the structure of the device is complex. Turntable scanning is more appropriate for large nucleic acid detection equipment with 48 or 96 holes. When used on small nucleic acid detection equipment, assembly will be difficult. There is also multi-channel scanning through flat scanning, in which the excitation and emission of each channel are integrated together. If there are 6 channels or even more channels, the stroke will be relatively large during the flat scanning process, which is not conducive to the miniaturization of nucleic acid detection equipment. Utility Model Content
[0004] The purpose of the utility model is to provide an optical detection system and nucleic acid detection equipment for nucleic acid detection equipment, in which the optical detection channels of the flat-scanning optical detection system are arranged side by side horizontally, thereby shortening the scanning stroke and facilitating the miniaturization of the nucleic acid detection equipment.
[0005] In order to achieve the above-mentioned object, the utility model provides an optical detection system for a nucleic acid detection device on the one hand, and the optical detection system comprises:
[0006] A sample holding member, used for holding a sample reagent;
[0007] a signal transmission component connected to the sample holding component and used for transmitting an optical signal;
[0008] The optical detection module comprises a movable mounting seat and a first detection channel component, a second detection channel component and a transmission member arranged in the movable mounting seat. The movable mounting seat is arranged on a lateral side of the signal transmission member and can move in the longitudinal direction. The first detection channel component and the second detection channel component are arranged at intervals in the lateral direction and are used to detect different fluorescent groups respectively; the transmission member is arranged on a side of the movable mounting seat close to the signal transmission member, and is used to focus the excitation light signal output from the optical detection module to the signal transmission member or collimate the emission light signal transmitted from the signal transmission member to the optical detection module.
[0009] In some embodiments, the second detection channel assembly is located between the first detection channel assembly and the transmission member, and the wavelength of the excitation light beam emitted by the first detection channel assembly is greater than the wavelength of the excitation light beam emitted by the second detection channel assembly.
[0010] In some embodiments, there are multiple optical detection modules, and the multiple optical detection modules are distributed in the longitudinal direction and aligned in the transverse direction.
[0011] In some embodiments, multiple optical detection modules include a first optical detection module, a second optical detection module and a third optical detection module. In the first optical detection module, the first detection channel component can emit an excitation light beam with a wavelength of 570nm to 590nm, and the second detection channel component is arranged between the first detection channel component and the transmission component, and can emit an excitation light beam with a wavelength of 418nm to 442nm; in the second optical detection module, the first detection channel component can emit an excitation light beam with a wavelength of 625nm to 635nm, and the second detection channel component is arranged between the first detection channel component and the transmission component, and can emit an excitation light beam with a wavelength of 483nm to 493nm; in the third optical detection module, the first detection channel component can emit an excitation light beam with a wavelength of 700nm to 710nm, and the second detection channel component is arranged between the first detection channel component and the transmission component, and can emit an excitation light beam with a wavelength of 535nm to 545nm.
[0012] In some embodiments, there are a plurality of sample holding members, a plurality of signal transmission members, the plurality of signal transmission members are disposed on the plurality of sample holding members in a one-to-one correspondence, and the plurality of signal transmission members are spaced apart and distributed in the longitudinal direction.
[0013] In some embodiments, the first detection channel component and the second detection channel component both include: a light-emitting component for emitting an excitation light beam; a collimating component, which is arranged on the light-emitting side of the light-emitting component and is used to collimate the excitation light beam; a first beam adjustment component, which is arranged on the light-emitting side of the collimating component and is used to adjust the excitation light beam, so that the excitation light beam passes through the transmission component and the signal transmission component and then irradiates the sample reagent, and the fluorescent group in the sample reagent will generate corresponding fluorescence after being excited by the excitation light beam; a second beam adjustment component, which is arranged on the lateral side of the first beam adjustment component away from the transmission component, and is used to adjust the fluorescent light beam transmitted to the optical detection module; a first focusing component, which is arranged on the light-emitting side of the second beam adjustment component and is used to focus the fluorescent light beam; and a detection component, which is arranged on the light-emitting side of the first focusing component, and is used to perform photoelectric conversion on the fluorescent light beam.
[0014] In some embodiments, the first detection channel assembly and the second detection channel assembly further include an emission filter disposed between the second beam adjustment component and the first focusing assembly and configured to filter the fluorescent beam.
[0015] In some embodiments, the emission filter is a bandpass filter or a combination of multiple bandpass filters.
[0016] In some embodiments, the signal transmission element is an optical fiber, and the effective diameter φ of the optical fiber is in the range of 1.0 mm-2.0 mm.
[0017] A second aspect of the utility model provides a nucleic acid detection device, which includes the above-mentioned optical detection system for nucleic acid detection equipment.
[0018] Through the above technical scheme, the utility model divides the optical detection module of the optical detection system into a first detection channel component and a second detection channel component, and the first detection channel component and the second detection channel component are laterally spaced apart, thereby reducing the longitudinal length of the optical detection, facilitating the spatial arrangement of the optical detection system, and meeting the miniaturization requirements of the nucleic acid detection equipment.
[0019] Other features and advantages of the embodiments of the present invention will be described in detail in the subsequent specific implementation section. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] The accompanying drawings are used to provide a further understanding of the embodiments of the present utility model and constitute a part of the specification. Together with the following specific embodiments, they are used to explain the embodiments of the present utility model, but do not constitute a limitation on the embodiments of the present utility model. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without creative work. In the drawings:
[0021] Figure 1 It is a schematic diagram of the structure of an optical detection system provided according to an embodiment of the utility model.
[0022] Description of Reference Numerals
[0023] 100 optical detection system 315 first focusing component
[0024] 10 Sample holder 316 Detection unit
[0025] 20 Signal transmission element 317 Emission filter
[0026] 30 First optical detection module 318 Excitation filter
[0027] 31 first detection channel component 32 second detection channel component
[0028] 311 Light-emitting parts 33 Transmitting parts
[0029] 312 Collimation assembly 34 Movable mounting seat
[0030] 313 first beam adjustment member 40 second optical detection module
[0031] 314 second beam adjustment member 50 third optical detection module DETAILED DESCRIPTION
[0032] The specific implementation of the present invention is described in detail below in conjunction with the accompanying drawings. It should be understood that the specific implementation described here is only used to illustrate and explain the present invention, and is not used to limit the present invention.
[0033] The optical detection system 100 for nucleic acid detection equipment according to the present invention is described below with reference to the accompanying drawings. Figure 1 FIG. 1 is a schematic diagram of the structure of an optical detection system 100 for a nucleic acid detection device provided according to an embodiment of the present utility model. The optical detection system 100 includes:
[0034] A sample holding member 10, used for holding a sample reagent;
[0035] A signal transmission member 20 connected to the sample holding member 10 and used for transmitting an optical signal;
[0036] The optical detection module includes a movable mounting seat 34 and a first detection channel component 31, a second detection channel component 32 and a transmission member 33 arranged in the movable mounting seat 34. The movable mounting seat 34 is arranged on a lateral side of the signal transmission member 20 and can move in the longitudinal direction. The first detection channel component 31 and the second detection channel component 32 are arranged at intervals in the lateral direction and are used to detect different fluorescent groups; the transmission member 33 is arranged on a side of the movable mounting seat 34 close to the signal transmission member 20, and is used to focus the excitation light signal output from the optical detection module to the signal transmission member 20, or collimate the emission light signal transmitted from the signal transmission member 20 to the optical detection module.
[0037] The sample container 10 can hold a sample reagent, which includes at least two fluorescent groups. The fluorescent groups can be combined with specific PCR products to locate and determine whether a specific PCR product exists in the sample reagent. When a specific PCR product exists, the fluorescent group will emit fluorescence when irradiated with light of a specific wavelength. Since it is separated from the quenching group, the fluorescence will be detected by the optical detection system. The signal transmission member 20 can be used to transmit optical signals, such as excitation light signals and emission light signals. The signal transmission member 20 can be an optical fiber. The optical detection module can output an optical signal of a specific wavelength and transmit it to the signal transmission member, and finally reach the sample reagent to excite the fluorescent group in the sample reagent to emit a fluorescent signal; the signal transmission member 20 transmits the fluorescent signal to the optical detection module, and the optical detection module determines whether the corresponding PCR product exists in the sample reagent according to the fluorescent signal. The optical detection module is provided with a first detection channel component 31 and a second detection channel component 32. The first detection channel component 31 and the second detection channel component 32 can respectively detect different fluorescent groups. In this embodiment, different fluorescent groups specifically refer to fluorescent groups suitable for different detection channels to determine whether a specific PCR product exists in the sample reagent. The movable mounting seat 34 can be driven by an external driving member to move longitudinally, so that the movable mounting seat 34 can drive the first detection channel assembly 31, the second detection channel assembly 32 and the transmission member 33 to move, so as to realize multi-sample scanning. The number of sample holders 10 can be multiple. When there are multiple samples to be tested, it is necessary to drive the movable mounting seat 34 to move longitudinally to scan and detect different samples. The transmission member 33 is arranged on the side of the movable mounting seat 34 close to the signal transmission member 20. When the first detection channel assembly 31 or the second detection channel assembly 32 emits a light signal, it is focused and coupled to the signal transmission member 20 through the transmission member 33. When the substance in the sample reagent emits fluorescence, the fluorescence is transmitted to the transmission member 33 through the signal transmission member 20. The transmission member 33 collimates the divergent fluorescence and converts the divergent fluorescence signal into parallel light and transmits it to the first detection channel assembly 31 or the second detection channel assembly 32 to determine the substance in the sample reagent. Specifically, the transmission member 33 can be a plano-convex lens or a lens group, and the movable mounting seat 34 can be an optical element mounting structure. Among them, the transverse direction and the longitudinal direction are as follows: Figure 1 shown.
[0038] The above-mentioned optical detection system 100 can detect sample reagents in multiple sample containers 10 by longitudinal flat scanning. The first detection channel assembly 31 and the second detection channel assembly 32 are distributed in a transverse interval, which reduces the longitudinal movement range of the optical detection module and also reduces the width occupied by the optical detection system 100 in the longitudinal direction, making it easy to arrange the optical detection system 100 as a whole and conducive to the miniaturized design of nucleic acid detection equipment.
[0039] In one embodiment, the second detection channel component 32 is located between the first detection channel component 31 and the transmission member 33, and the wavelength of the excitation light beam emitted by the first detection channel component 31 is greater than the wavelength of the excitation light beam emitted by the second detection channel component 32. Because both the first detection channel component 31 and the second detection channel component 32 need to pass through the transmission member 33, during the light transmission process, it is necessary to adjust the path of the light beams of different wavelengths. Therefore, the wavelength of the excitation light beam emitted by the first detection channel component 31 is set to be greater than the wavelength of the laser light beam emitted by the second detection channel component 32. For example, a long-wave pass dichroic mirror is set in the light beam propagation path so that the fluorescence with a shorter wavelength is reflected by the long-wave pass filter to the second detection channel component 32, and the longer fluorescence passes through the long-wave pass filter into the first detection channel component 31. Among them, the long-wave pass dichroic mirror allows light within a certain wavelength range (larger wavelength) to pass through, and reflects light within another wavelength range (smaller wavelength).
[0040] In one embodiment, Figure 1 As shown, there are multiple optical detection modules, which are distributed longitudinally and aligned transversely. Nucleic acid detection equipment can usually detect a variety of different PCR products, and each PCR product requires an optical detection module. Therefore, multiple optical detection modules can be distributed longitudinally and aligned transversely to detect multiple PCR products and achieve multi-target detection.
[0041] In one embodiment, the plurality of optical detection modules include a first optical detection module 30, a second optical detection module 40 and a third optical detection module 50. In the first optical detection module 30, the first detection channel component 31 can emit an excitation light beam with a wavelength of 570nm to 590nm, and the second detection channel component 32 is arranged between the first detection channel component 31 and the transmission component 33 and can emit an excitation light beam with a wavelength of 418nm to 442nm; in the second optical detection module 40, the first detection channel component 31 can emit an excitation light beam with a wavelength of 625nm to 635nm, and the second detection channel component 32 is arranged between the first detection channel component 31 and the transmission component 33 and can emit an excitation light beam with a wavelength of 483nm to 493nm; in the third optical detection module 50, the first detection channel component 31 can emit an excitation light beam with a wavelength of 700nm to 710nm, and the second detection channel component 32 is arranged between the first detection channel component 31 and the transmission component 33 and can emit an excitation light beam with a wavelength of 535nm to 545nm. During the detection process, in order to avoid interference between multiple optical signals to cause analysis errors, the first detection channel component 31 and the second detection channel component 32 in the same optical detection module cannot be excited at the same time, and the wavelengths of the excitation light beams of the first detection channel component 31 and the second detection channel component 32 in the same optical detection module should be significantly different to prevent the optical adjustment component from being unable to separate light of different wavelengths. Specifically, the first detection channel component is a detection channel component of a ROX channel fluorescent group, and the excitation wavelength of the ROX channel fluorescent group is about 580nm, and the emission wavelength is about 610nm; the second detection channel component is a detection channel component of an Atto 425 channel fluorescent group, and the excitation wavelength of the Atto 425 channel fluorescent group is about 439nm, and the emission wavelength is about 489nm; the third detection channel component is a detection channel component of a Cy5 channel fluorescent group, and the excitation wavelength of the Cy5 channel fluorescent group is about 640nm, and the emission wavelength is about 670nm; the fourth detection channel component is a detection channel component of a FAM channel fluorescent group, and the excitation wavelength of the FAM channel fluorescent group is about 492nm, and the emission wavelength is about 518nm; the fifth detection channel component is a detection channel component of a Quasar 705 channel fluorescent group, and the excitation wavelength of the Quasar 705 channel fluorescent group is about 690nm, and the emission wavelength is about 705nm; the sixth detection channel component is a detection channel component of a VIC channel fluorescent group, and the excitation wavelength of the VIC channel fluorescent group is about 526nm, and the emission wavelength is about 543nm. Among them, the excitation wavelength and emission wavelength of the fluorescent group will vary depending on the manufacturer and application scenario.
[0042] In one embodiment, Figure 1As shown, there are multiple sample holders 10, and there are multiple signal transmission members 20. The multiple signal transmission members 20 are arranged on the multiple sample holders 10 in a one-to-one correspondence, and the multiple signal transmission members 20 are distributed at intervals along the longitudinal direction. With this distribution method, when the movable mounting seat 34 moves along the longitudinal direction, the light coming out of the transmission member 33 of the optical detection module can enter each optical fiber in turn, and then be transmitted into the sample holder 10 by the optical fiber. The fluorescent group in the sample reagent in the sample holder 10 will produce corresponding fluorescence after being excited by the excitation light beam, and the fluorescence is then transmitted to the optical detection module through the optical fiber to complete the optical detection. The above test steps are simple and easy to operate, which is convenient for the nucleic acid detection equipment to perform the detection work.
[0043] In one embodiment, Figure 1 As shown, the first detection channel component 31 and the second detection channel component 32 both include: a light-emitting component 311, used to emit an excitation light beam; a collimating component 312, which is arranged on the light-emitting side of the light-emitting component 311 and used to collimate the excitation light beam; a first beam adjusting component 313, which is arranged on the light-emitting side of the collimating component 312 and used to adjust the route of the excitation light beam, so that the excitation light beam passes through the transmission component 33 and the signal transmission component 20 and then irradiates the sample reagent, and the fluorescent group in the sample reagent generates a corresponding fluorescent light beam after being excited by the excitation light beam; a second beam adjusting component 314, which is arranged on the lateral side of the first beam adjusting component 313 away from the transmission component 33, and is used to adjust the fluorescent light beam transmitted to the optical detection module; a first focusing component 315, which is arranged on the light-emitting side of the second beam adjusting component 314 and is used to focus the fluorescent light beam; and a detection component 316, which is arranged on the light-emitting side of the first focusing component 315, and is used to perform photoelectric conversion on the fluorescent light beam.
[0044] The light emitting element 311 (such as an LED lamp) can emit an excitation light beam, wherein the light beams emitted by different optical detection modules have different colors, that is, the wavelengths of the emitted excitation light beams are different. The collimating component 312 collimates the light beam emitted by the light emitting element 311 and converts the divergent light into parallel light. The first beam adjusting component 313 is arranged on the light emitting side of the light emitting element 311, and can adjust the light beam so that the excitation light beam from the collimating component 312 goes in the direction of the transmission component 33, and the transmission component 33 focuses the excitation light beam onto the signal transmission component 20. In this embodiment, the light emitting element 311, the collimating component 312, the first beam adjusting component 313 and the transmission component 33 together constitute an excitation channel component. The second beam adjusting component 314 is arranged on the lateral side of the first beam adjusting component 313 away from the transmission component 33. The second beam adjusting component 314 can adjust the route of the fluorescent light beam so that the fluorescent light beam propagates in the direction of the detection component 316. The first focusing component 315 is arranged at the light outlet of the second beam adjusting member 314, and the second beam adjusting member 314 allows the fluorescent light beam to enter the first focusing component 315. The first focusing component 315 focuses the parallel fluorescent light beam, and the detection member 316 performs photoelectric conversion on the focused fluorescent light beam. In this embodiment, the second beam adjusting member 314, the first focusing component 315 and the detection member 316 together constitute a detection channel component. Among them, the detection member 316 can be a silicon photodiode, which converts the received fluorescent signal into an electrical signal and transmits it to an external controller. The collimating component 312 can be a combination of two positive lenses, and the first focusing component 315 can be a double convex lens. By using the above-mentioned first detection channel component 31 and the second detection channel component 32, the optical path of the excitation light beam and the fluorescent light beam can be adjusted, which is convenient for the miniaturized design of the optical detection module.
[0045] In one embodiment, the second beam adjustment component 314 of the second detection channel assembly 32 is a long-wave pass dichroic mirror, which can allow light signals greater than a preset wavelength to pass through and reflect fluorescent signals less than the preset wavelength to the emission filter 317 of the second detection channel assembly 32.
[0046] In one embodiment, the second beam adjustment component 314 of the first detection channel assembly 31 is a reflector, which can reflect the fluorescent signal to the emission filter 317 of the first detection channel assembly 31 .
[0047] Specifically, after the light emitting member 311 of the first optical detection channel assembly 31 emits a light signal, the light signal sequentially passes through the collimating assembly 312 of the first detection channel assembly 31, the first beam adjusting member 313 of the first detection channel assembly 31, the second beam adjusting member 314 of the second detection channel assembly 32, the first beam adjusting member 313 of the second detection channel assembly 32, the transmission member 33 and the signal transmission member 20, and then enters the sample tube 10. After the fluorescent group in the sample reagent is excited to emit a fluorescent signal, the fluorescent signal sequentially passes through the signal transmission member 20, the transmission member 33, the first beam adjusting member 313 of the second detection channel assembly 32, the second beam adjusting member 314 of the second detection channel assembly 32, the first beam adjusting member 313 of the first detection channel assembly 31, the second beam adjusting member 314 of the first detection channel assembly 31, the emission filter 317 of the first detection channel assembly 31 and the first focusing assembly 315 of the first detection channel assembly 31, and finally reaches the detection member 316 of the first detection channel assembly 31.
[0048] After the light-emitting component 311 of the second optical detection channel assembly 32 emits a light signal, the light signal sequentially passes through the collimating component 312 of the second detection channel assembly 32, the first beam adjusting component 313 of the second detection channel assembly 32, the transmission component 33 and the signal transmission component 20 before entering the sample tube 10; after the fluorescent group in the sample reagent is excited to emit a fluorescent signal, the fluorescent signal sequentially passes through the signal transmission component 20, the transmission component 33, the first beam adjusting component 313 of the second detection channel assembly 32, the second beam adjusting component 314 of the second detection channel assembly 32, the emission filter 317 of the second detection channel assembly 32 and the first focusing component 315 of the second detection channel assembly 32 before finally reaching the detection component 316 of the second detection channel assembly 32.
[0049] In one embodiment, an excitation filter 318 is further disposed between the collimating assembly 312 and the first beam adjusting member 313 . The excitation filter 318 is disposed so that only a preset wavelength band of light emitted by the light emitting member 311 can be transmitted to the first beam adjusting member 313 .
[0050] In one embodiment, the first detection channel assembly 31 and the second detection channel assembly 32 also include an emission filter 317 disposed between the second beam adjustment member 314 and the first focusing member 315 and used to filter the fluorescent light beam. When the detection member 316 of the optical detection module detects the fluorescent light beam, in order to avoid detection errors or crosstalk, it is necessary to filter the fluorescent light beam so that the fluorescent light beam within a preset wavelength range enters the detection member 316. If the wavelength band of the fluorescent light beam is not within the passband range of the emission filter 317, it cannot enter the detection member 316, and it can be determined that the corresponding substance does not exist in the sample reagent; if the fluorescent light beam has light within the wavelength band, it can pass through the emission filter 317 and enter the detection member 316, so that the detection member 316 determines that the corresponding substance exists in the sample reagent. The above-mentioned optical detection system 100 can make the detection result of the detection member 316 more accurate and avoid the occurrence of detection errors. Furthermore, in this embodiment, the second beam adjusting member 314 , the emission filter 317 , the first focusing assembly 315 and the detection member 316 together constitute a detection channel assembly.
[0051] In one embodiment, the emission filter 317 is a bandpass filter or a combination of multiple bandpass filters. The combination of multiple (such as 2) bandpass filters can achieve better filtering effects. Using one bandpass filter is less expensive and takes up less space. Users can arrange different filters to filter the fluorescent light beam as needed.
[0052] In one embodiment, the signal transmission element 20 is an optical fiber, and the effective diameter φ of the optical fiber ranges from 1.0 mm to 2.0 mm. The optical fiber within the effective diameter range has high sensitivity and is highly compatible with the optical detection module of the utility model, and can better detect sample reagents.
[0053] In one embodiment, a nucleic acid detection device is provided, and the nucleic acid detection device includes the above-mentioned optical detection system 100 for nucleic acid detection device.
[0054] In the description of the present invention, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, "plurality" means at least two, such as two, three, etc., unless otherwise clearly and specifically defined.
[0055] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral one; it can be a mechanical connection, an electrical connection, or communication with each other; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.
[0056] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.
[0057] Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations of the present invention. Ordinary technicians in the field can change, modify, replace and modify the above embodiments within the scope of the present invention.
Claims
1. An optical detection system for a nucleic acid detection device, characterized in that: The optical detection system (100) comprises: A sample holding member (10) for holding a sample reagent; A signal transmission component (20) connected to the sample holding component (10) and used for transmitting an optical signal; An optical detection module comprises a movable mounting seat (34) and a first detection channel component (31), a second detection channel component (32) and a transmission member (33) arranged in the movable mounting seat (34); the movable mounting seat (34) is arranged on a lateral side of the signal transmission member (20) and is movable in a longitudinal direction; the first detection channel component (31) and the second detection channel component (32) are arranged in a lateral spacing and are used to detect different fluorescent groups; the transmission member (33) is arranged on a side of the movable mounting seat (34) close to the signal transmission member (20) and is used to focus an excitation light signal output from the optical detection module to the signal transmission member (20) or to collimate an emission light signal transmitted from the signal transmission member (20) to the optical detection module.
2. The optical detection system for nucleic acid detection equipment according to claim 1, characterized in that: The second detection channel component (32) is located between the first detection channel component (31) and the transmission component (33), and the wavelength of the excitation light beam emitted by the first detection channel component (31) is greater than the wavelength of the excitation light beam emitted by the second detection channel component (32).
3. The optical detection system for nucleic acid detection equipment according to claim 1, characterized in that: There are multiple optical detection modules, and the multiple optical detection modules are distributed in the longitudinal direction and aligned in the transverse direction.
4. The optical detection system for nucleic acid detection equipment according to claim 3, characterized in that: The plurality of optical detection modules include a first optical detection module (30), a second optical detection module (40) and a third optical detection module (50); in the first optical detection module (30), the first detection channel component (31) can emit an excitation light beam with a wavelength of 570nm to 590nm; the second detection channel component (32) is arranged between the first detection channel component (31) and the transmission component (33) and can emit an excitation light beam with a wavelength of 418nm to 442nm; in the second optical detection module (40), the first detection channel component (31) can emit an excitation light beam with a wavelength of 570nm to 590nm; The second detection channel component (32) is arranged between the first detection channel component (31) and the transmission component (33) and can emit an excitation light beam with a wavelength of 625nm to 635nm. The second detection channel component (32) is arranged between the first detection channel component (31) and the transmission component (33) and can emit an excitation light beam with a wavelength of 483nm to 493nm. In the third optical detection module (50), the first detection channel component (31) can emit an excitation light beam with a wavelength of 700nm to 710nm, and the second detection channel component (32) is arranged between the first detection channel component (31) and the transmission component (33) and can emit an excitation light beam with a wavelength of 535nm to 545nm.
5. The optical detection system for nucleic acid detection equipment according to claim 1, characterized in that: The number of the sample holding parts (10) is multiple, the number of the signal transmission parts (20) is multiple, the multiple signal transmission parts (20) are arranged on the multiple sample holding parts (10) in a one-to-one correspondence, and the multiple signal transmission parts (20) are distributed at intervals along the longitudinal direction.
6. The optical detection system for nucleic acid detection equipment according to claim 1, characterized in that: The first detection channel component (31) and the second detection channel component (32) both include: A light emitting element (311) for emitting an excitation light beam; A collimating component (312) is arranged on the light-emitting side of the light-emitting element (311) and is used to collimate the excitation light beam; A first beam adjustment member (313) is arranged on the light-emitting side of the collimating component (312) and is used to adjust the excitation light beam so that the excitation light beam passes through the transmission member (33) and the signal transmission member (20) and then irradiates the sample reagent, and the fluorescent group in the sample reagent generates corresponding fluorescence after being excited by the excitation light beam; A second beam adjustment member (314) is arranged on a lateral side of the first beam adjustment member (313) away from the transmission member (33), and is used to adjust the fluorescent light beam transmitted to the optical detection module; A first focusing component (315) is arranged on the light-emitting side of the second beam adjusting component (314) and is used to focus the fluorescent light beam; The detection element (316) is arranged on the light-emitting side of the first focusing component (315) and is used to perform photoelectric conversion on the fluorescent light beam.
7. The optical detection system for nucleic acid detection equipment according to claim 6, characterized in that: The first detection channel assembly (31) and the second detection channel assembly (32) further include an emission filter (317) disposed between the second beam adjustment member (314) and the first focusing assembly (315) and used for filtering the fluorescent light beam.
8. The optical detection system for nucleic acid detection equipment according to claim 7, characterized in that: The emission filter (317) is a bandpass filter or a combination of multiple bandpass filters.
9. The optical detection system for nucleic acid detection equipment according to any one of claims 1 to 8, characterized in that: The signal transmission element (20) is an optical fiber, and the effective diameter φ of the optical fiber is in the range of 1.0 mm to 2.0 mm.
10. A nucleic acid detection device, characterized in that: The nucleic acid detection device comprises the optical detection system (100) for nucleic acid detection device according to any one of claims 1 to 9.