Traditional Chinese medicine microorganism high-precision detection equipment

Abstract

The utility model provides traditional Chinese medicine microorganism high-precision detection equipment which comprises a cover dismounting device, a detection device, an injection liquid adding device, a pressure maintaining device and a liquid transferring device, the cover dismounting device comprises a cover dismounting linear module and a cover dismounting driver, and a clamping device is mounted on the cover dismounting linear module; the injection liquid adding device comprises a plurality of liquid adding steel needles, and each liquid adding steel needle is independently connected with a liquid adding pipeline; the pipetting device comprises a pipetting linear module and a pipetting driver; a pipetting gun and a follow-up frame are mounted on the pipetting linear module; the pressure maintaining device comprises a pressure maintaining cover plate and an exhaust pipe. A compression spring is arranged between the upper end face of the pressure maintaining cover plate and the lower end face of the cantilever. Gas in the gas blowing pipe is provided by the nitrogen tank, and the electromagnetic valve is controlled by the electric control cabinet to be opened and closed to realize on-off of gas. According to the invention, the automatic control of the microbiological detection process is realized, the sample treatment accuracy and the laboratory production efficiency are high, the interference of human factors is reduced, and especially for large-scale detection, the efficiency is high and the cost is low.

Description

Technical Field

[0001] The invention belongs to the technical field of microbial detection, and in particular relates to a high-precision detection device for traditional Chinese medicine microorganisms. Background Art

[0002] Microbial limit inspection is an important part of the safety control of traditional Chinese medicine. In the production process of traditional Chinese medicine products, it is an indispensable step to test the microbial limits of multiple links. The traditional detection method mainly adopts the culture counting method. Although the results are intuitive, this method has a long detection cycle, is susceptible to the risk of interference from exogenous pollutants, and cannot test uncultivable microorganisms, which has great limitations. Although there are schemes for microbial detection using Raman spectroscopy technology in the prior art, it mainly relies on manual assisted operation, which is labor-intensive, inefficient, and may cause uncontrollable factors, resulting in distorted test results. Therefore, it is necessary to improve the automation of the detection equipment. Summary of the invention

[0003] In view of this, the present invention aims to overcome the defects in the prior art and proposes a high-precision detection device for microorganisms in traditional Chinese medicine.

[0004] To achieve the above object, the technical solution created by the present invention is implemented as follows:

[0005] A high-precision detection device for traditional Chinese medicine microorganisms, comprising:

[0006] The capping device comprises a capping fixing plate, a capping linear module and a capping driver for driving the capping linear module to perform an action. A clamping device is installed on the capping linear module, and the clamping device is driven by the capping linear module to move in a vertical direction. The capping fixing plate is arranged below the capping linear module, and a working head for fixing the body of the sample tube is provided at its front end.

[0007] The detection device comprises a mounting frame, on which a screw slide is provided, and on which a Raman spectrometer is installed;

[0008] The injection liquid adding device comprises a fixed plate, on which a plurality of liquid adding steel needles are installed, each of which is individually connected to a liquid adding pipeline; a bottle cap detection device is provided on the fixed plate;

[0009] The liquid transfer device comprises a liquid transfer linear module and a liquid transfer driver for driving the liquid transfer linear module to perform an action, a liquid transfer gun and a follower frame are installed on the liquid transfer linear module, and the liquid transfer gun and the follower frame are driven by the liquid transfer linear module to move synchronously in the vertical direction;

[0010] The pressure-maintaining device comprises a pressure-maintaining cover plate installed on the cantilever of the follower frame, a pressure-maintaining nozzle in the middle of the pressure-maintaining cover plate, and an air blowing pipe connected with the air hole in the pressure-maintaining nozzle. A compression spring is arranged between the upper end surface of the pressure-maintaining nozzle and the lower end surface of the cantilever. The gas in the air blowing pipe is provided by a nitrogen tank, and the opening and closing of the electromagnetic valve is controlled by the electric control cabinet to realize the air flow interruption.

[0011] The cover removal device, the detection device, the injection and liquid adding device, the pressure maintaining device and the liquid transfer device are all arranged on the fixed frame.

[0012] Furthermore, the clamping device includes a base and a plurality of clamping claws on the lower side of the base, and is provided with a cover removal driver for driving the clamping claws to move.

[0013] Furthermore, the clamping device includes 3-5 clamping jaws, and a clamping rod is provided at the lower end of each clamping jaw.

[0014] Furthermore, the follower frame is an L-shaped structure.

[0015] Furthermore, a Raman label molecule liquid-adding steel needle, an alkaline elution solution liquid-adding steel needle, and a Raman enhancement signal molecule liquid-adding steel needle are installed on the fixed plate.

[0016] Furthermore, the liquid transfer linear module is installed on the fixed frame through an auxiliary frame; the auxiliary frame is a U-shaped structure.

[0017] Furthermore, a Z-axis fixed plate is provided on the liquid transfer linear module, a follower frame is arranged on the side of the Z-axis fixed plate, and a liquid transfer gun is installed on the surface of the Z-axis fixed plate.

[0018] Furthermore, a sealing gasket is provided on the lower end face of the pressure-maintaining nozzle, and its mouth is consistent with the size of the filter. The pipetting linear module is driven by the pipetting driver to drive the pressure-maintaining nozzle to squeeze the filter seat downward, so that the sealing gasket is in close contact with the filter seat to effectively prevent gas leakage.

[0019] Compared with the prior art, the present invention has the following advantages:

[0020] The invention provides a cover removal device, a detection device, an injection and liquid adding device, a pressure maintaining device and a liquid transfer device on a fixed frame, so as to automatically complete repetitive tasks such as removing the plug of a sample tube, liquid transfer, sample loading and detection, thereby realizing automated control of the microbial detection process, and achieving high accuracy of sample processing and high laboratory production efficiency, especially for large-scale detection, with high efficiency and low cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The drawings constituting part of the present invention are used to provide a further understanding of the present invention. The exemplary embodiments and descriptions of the present invention are used to explain the present invention and do not constitute an improper limitation on the present invention. In the drawings:

[0022] Figure 1A schematic diagram of the structure created by the present invention;

[0023] Figure 2 This is a schematic diagram of the capping working head fixing the sample tube body in the invention;

[0024] Figure 3 A schematic diagram of the cover removal device and the detection device in the invention;

[0025] Figure 4 It is a schematic diagram of the injection and liquid adding device part in the invention;

[0026] Figure 5 It is a partial schematic diagram of the liquid transfer device and the pressure maintaining device in the invention. DETAILED DESCRIPTION

[0027] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features in the embodiments may be combined with each other.

[0028] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. In addition, the terms "first", "second", etc. are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, features defined as "first", "second", etc. may explicitly or implicitly include one or more of the features. In the description of the present invention, unless otherwise specified, "multiple" means two or more.

[0029] In the description of the invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the invention can be understood according to specific circumstances.

[0030] The present invention will be described in detail below with reference to the accompanying drawings and in combination with embodiments.

[0031] A high-precision detection device for microorganisms in traditional Chinese medicine, such as Figures 1 to 5 As shown, including:

[0032] The capping device 1 comprises a capping fixing plate 101, a capping linear module 102 and a capping driver 103 for driving the capping linear module to perform an action. A clamping device 104 is installed on the capping linear module, and the clamping device 104 is driven by the capping linear module 102 to move in the vertical direction. The capping fixing plate 101 is arranged below the capping linear module 102, and a working head 105 for fixing the body of the sample tube is provided at its front end. Specifically, an arc-shaped working surface 106 is provided on the working head, and a notch 107 is provided on the working surface. The turntable driver drives the turntable to move to the leftmost end so that the notch is just fixed on the lower half of the body of the exposed sample tube to achieve fixation;

[0033] The detection device 2 comprises a mounting frame 201, on which a screw slide 203 is provided, and on which a Raman spectrometer 202 is installed;

[0034] The injection liquid adding device 3 comprises a fixed plate 301, on which a plurality of liquid adding steel needles 302 are mounted, each of which is individually connected to a liquid adding pipeline 303; and a bottle cap detection device 304 is provided on the fixed plate;

[0035] The liquid transfer device 4 includes a liquid transfer linear module 401 and a liquid transfer driver 402 for driving the liquid transfer linear module to perform an action. A liquid transfer gun 403 and a follower frame 404 are installed on the liquid transfer linear module. The liquid transfer linear module 401 drives the liquid transfer gun 403 and the follower frame 404 to move synchronously in the vertical direction.

[0036] The pressure-maintaining device 5 comprises a pressure-maintaining cover plate 501 mounted on the cantilever of the follower frame 404, a pressure-maintaining nozzle 502 in the middle of the pressure-maintaining cover plate, and an air blowing pipe 503 connected to the air hole in the pressure-maintaining nozzle 502. A compression spring 504 is arranged between the upper end surface of the pressure-maintaining nozzle 502 and the lower end surface of the cantilever. The gas in the air blowing pipe 503 is provided by a nitrogen tank, and the opening and closing of the solenoid valve is controlled by the electric control cabinet to realize the interruption of the air flow. When the follower frame 404 moves downward, the pressure-maintaining cover plate 501 presses the top opening of the filter to maintain the seal, and the compression spring 504 can ensure that the pipette gun 403 can continue to move downward when the pressure-maintaining cover plate 501 is in contact with the filter, without causing interference or damage to components, thereby effectively improving the adaptability of the pressure-maintaining device.

[0037] The cover removal device, the detection device, the injection and liquid adding device, the pressure maintaining device and the liquid transfer device are all arranged on the fixed frame 6.

[0038] The clamping device 104 includes a base 108 and several clamping claws 109 on the lower side of the base, and is provided with a cover removal driver 103 for driving the clamping claws. Usually, the clamping device 104 includes 3-5 clamping claws 109, and a clamping rod 110 is provided at the lower end of each clamping claw. As an example, the clamping rod 110 can be a nylon rod or a rubber rod. The clamping device 104 can be a commercially available electric clamping claw product, which can clamp the sample tube plug, and under the drive of the cover removal linear module 102, the sample tube plug can be pulled out.

[0039] A sealing gasket 505 is provided on the lower end surface of the pressure-maintaining nozzle 502, and its mouth is consistent with the size of the filter. The liquid transfer driver 402 drives the liquid transfer linear module 401 to drive the pressure-maintaining nozzle 502 to press the filter seat downward, so that the sealing gasket 505 is in close contact with the filter seat to effectively prevent gas leakage.

[0040] The follower frame 404 is in an L-shaped structure. The fixed plate is equipped with a Raman tag molecule adding needle, an alkaline elution solution adding needle, and a Raman enhanced signal molecule adding needle. For example, the fixed plate 301 is equipped with an MBPA adding needle 305, an NH4HCO3 solution adding needle 306, and an Au@AgNPs solution adding needle 307. The pipetting linear module 401 is installed on the fixed frame 6 through the auxiliary frame 7; the auxiliary frame 7 is in an L-shaped structure. The Z-axis fixed plate 405 is provided on the (slider) of the pipetting linear module 401, the follower frame 404 is arranged on the side of the Z-axis fixed plate 405, and the pipette gun 403 is installed on the surface of the Z-axis fixed plate.

[0041] A sealing gasket 505 is provided on the lower end face of the pressure-maintaining cover plate 501. When the pipetting linear module 401 drives the pipetting gun 403 to move downward, the pressure-maintaining cover plate 501 on the follower frame 404 will gradually approach and press the top opening of the filter. The gas release rate and flow rate are adjusted by controlling the nitrogen tank cylinder valve to make the gas pressure in the tank 0.01Mpa-0.6Mpa. The PLC drives the solenoid valve to open and continuously delivers gas to the blowing pipe to achieve blowing. Then, the pressure is maintained by controlling the size of the opening and closing of the solenoid valve. The pressure-maintaining and blowing process can complete the entire filtration in about 60 seconds.

[0042] During application, the user adds the Chinese medicine sample to be tested into a sterile sample tube, the cover removal device 1 automatically removes the sample tube cover, the pipette gun 403 draws the sample liquid in the sample tube and adds it into the filter, the pressure-maintaining device 5 presses the top opening of the filter and then evacuates the air, thereby achieving rapid filtration, and injects 0.5-5mL of 4-mercaptophenylboronic acid Raman label molecules onto the filter membrane through the liquid-adding steel needle 305, and incubates for 10-30 minutes, and the pressure-maintaining device 5 performs suction filtration again, and then the liquid-adding steel needle 306 and the liquid-adding steel needle 307 sequentially add 2-10mL of ammonium bicarbonate alkaline eluent and 0.3-3mL of Au@AgNPs Raman enhanced signal molecule sol solution, and the pressure-maintaining device 5 performs suction filtration each time the reagent is added. The filter membrane in the filter is dried naturally, and finally the laser 203 at the lower end of the Raman spectrometer 202 emits laser, and the detection device 2 performs spectrum acquisition.

[0043] The specific working process is that the turntable moves linearly to the leftmost end, so that the notch 107 in the center of the arc-shaped screw-cover fixing plate 101 firmly clamps the upper half of the exposed sealed sample tube body of acupoint No. 1, and the clamping claw rod 110 of the clamping device 104 clamps the cover of the sample tube containing the Chinese medicine sample liquid below, and the clamping claw rod 110 moves upward with the upward sliding of the cap removal linear module 102 (slider) to pull out the tube cover, and then the uncovered Chinese medicine sample liquid sample tube is sent to the bottom of the bottle cap detection device 304 for uncovered sample tube screening. If there is no cover, the screening is qualified. If the screening is unqualified, the result will be reported to the HMI system and the alarm device will be triggered to sound the whistle, the equipment will stop working, and the staff will intervene in time. After that, the No. 1 acupoint displacement liquid pipette head will be moved to the liquid collection position (just below the pipetting device), The pipette gun 403 picks up a pipette tip and delivers the uncovered Chinese medicine sample liquid tube No. 1 to the liquid collection position (directly below the pipetting device). The pipette driver 402 drives the pipette gun 403 to move downward to the corresponding position of the Chinese medicine sample liquid tube. The pipette tip is used to absorb 1 mL of Chinese medicine sample liquid in the corresponding sample tube, and the sample liquid is transferred to the filter (filter membrane). The pipette driver 402 then drives the pipette gun 403 to put the tip back to the original acupuncture point. The uncovered Chinese medicine sample liquid tube is delivered to the bottom of the decapping device 1. Similarly, the decapping driver 103 drives the covered clamping claw rod 110 to move downward, and then close the tube cover. Finally, the pressure maintaining device 5 is aligned with the filter for suction filtration to achieve rapid filtration. This process is repeated many times to add the Chinese medicine sample liquid in all sample tubes to the filter membrane in turn.

[0044] Then, the 4-mercaptophenylboric acid solution is transported to the liquid adding pipeline 303 through the injection pump, and then 0.5 mL of 4-mercaptophenylboric acid is added to the filter membrane of the filter through the liquid adding steel needle 305. After standing and incubating for 15 minutes, the 4-mercaptophenylboric acid and the bacteria are fully combined. Then, the filter is vacuumed and filtered again through the pressure maintaining device 5. After that, 3 mL of ammonium bicarbonate eluent and 1 mL of Au@AgNPs sol solution are added to the filter membrane of the filter through the corresponding liquid adding steel needle 306 and the liquid adding steel needle 307. The pressure maintaining device will perform vacuum filtration every time the reagent is added.

[0045] Usually, a rotating device, a handheld movable touch screen HMI and an electric control cabinet (PLC is installed in the electric control cabinet) can be set on the side of the detection equipment. A turntable is installed on the rotating device. The filter, the pipette tip and the sample tube are arranged on the turntable of the rotating device. The filter, the pipette tip and the sample tube are moved to the desired position (that is, moved to the bottom of the corresponding working device) by driving the rotation of the rotating device. It should be noted that as long as the fixed frame 6 is installed on the side of the turntable, and then each working device can be aligned with the corresponding working position when working. As an example, 12 acupuncture points can be set along the circumferential direction of the turntable, each acupuncture point is inserted with a sample tube, and a pipette tip and a filter are provided corresponding to each acupuncture point.

[0046] The invention realizes the automatic control of the microbial detection process by arranging a cover removal device, a detection device, an injection and liquid addition device, a pressure-maintaining device and a liquid transfer device on a fixed frame, automatically completing repetitive tasks such as removing the sample tube plug, liquid transfer, sample loading and detection, and realizing the automatic control of the microbial detection process, the accuracy of sample processing and the laboratory production efficiency are high, and the interference of human factors is reduced, especially for large-scale detection, with high efficiency and low cost. In addition, an abnormal alarm program can be set through the HMI to deal with abnormal situations such as cover removal and liquid addition during sample detection, so as to ensure the continuous and stable operation of the detection system.

[0047] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A high-precision detection device for traditional Chinese medicine microorganisms, characterized in that: include: The cover removal device comprises a cover removal linear module and a cover removal driver for driving the cover removal linear module to perform an action, wherein a clamping device is installed on the cover removal linear module, and the clamping device is driven by the cover removal linear module to move in a vertical direction; The detection device comprises a mounting frame, on which a screw slide is provided, and on which a Raman spectrometer is installed; The injection liquid adding device comprises a fixed plate, on which a plurality of liquid adding steel needles are installed, and each liquid adding steel needle is individually connected to a liquid adding pipeline; The liquid transfer device comprises a liquid transfer linear module and a liquid transfer driver for driving the liquid transfer linear module to perform an action, a liquid transfer gun and a follower frame are installed on the liquid transfer linear module, and the liquid transfer gun and the follower frame are driven by the liquid transfer linear module to move synchronously in the vertical direction; The pressure-maintaining device comprises a pressure-maintaining cover plate installed on the cantilever of the follower frame, a pressure-maintaining nozzle in the middle of the pressure-maintaining cover plate, and an air blowing pipe connected with the air hole in the pressure-maintaining nozzle. A compression spring is arranged between the upper end surface of the pressure-maintaining nozzle and the lower end surface of the cantilever. The gas in the air blowing pipe is provided by a nitrogen tank, and the opening and closing of the electromagnetic valve is controlled by the electric control cabinet to realize the air flow interruption. The cover removal device, the detection device, the injection and liquid adding device, the pressure maintaining device and the liquid transfer device are all arranged on the fixed frame.

2. A high-precision detection device for traditional Chinese medicine microorganisms according to claim 1, characterized in that: The clamping device comprises a base and a plurality of clamping claws on the lower side of the base, and is provided with a cover removing driver for driving the clamping claws to move.

3. A high-precision detection device for microorganisms in traditional Chinese medicine according to claim 2, characterized in that: The clamping device comprises 3-5 clamping jaws, and a clamping rod is provided at the lower end of each clamping jaw.

4. A high-precision detection device for microorganisms in traditional Chinese medicine according to claim 1, characterized in that: The follower frame is in an L-shaped structure.

5. A high-precision detection device for microorganisms in traditional Chinese medicine according to claim 1, characterized in that: A Raman label molecule liquid adding steel needle, an alkaline elution solution liquid adding steel needle, and a Raman enhanced signal molecule liquid adding steel needle are installed on the fixed plate.

6. A high-precision detection device for microorganisms in traditional Chinese medicine according to claim 1, characterized in that: The liquid transfer linear module is installed on the fixed frame through the auxiliary frame; the auxiliary frame is a U-shaped structure.

7. A high-precision detection device for microorganisms in traditional Chinese medicine according to claim 1, characterized in that: A Z-axis fixed plate is provided on the liquid transfer linear module, a follower frame is arranged on the side of the Z-axis fixed plate, and a liquid transfer gun is installed on the surface of the Z-axis fixed plate.

8. The high-precision detection device for microorganisms in traditional Chinese medicine according to claim 1, characterized in that: A sealing gasket is arranged on the lower end surface of the pressure-maintaining nozzle, and the nozzle opening thereof matches the size of the filter.

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