Quantitative separate injection device of DNA synthesizer

Abstract

The utility model discloses a quantitative separate injection device of a DNA (deoxyribonucleic acid) synthesizer, which belongs to the technical field of DNA synthesizers and comprises a fixing frame, a positioning component is arranged on the surface of the fixing frame, a sliding groove is arranged on the surface of the inner wall of the fixing frame, a sliding block is embedded in the sliding groove, and an adjusting component is arranged in the sliding block. Under the elastic action of a second spring in a side seat on a sleeve block, a positioning rod and a positioning groove are tightly inserted, so that a placing pipe can be firmly positioned right below a separate injection glass pipe, and further separate injection of the device is more accurate; through the arrangement of the adjusting assembly, the rotating screw rod is in meshed connection with the screw rod and the transmission blocks, due to the fact that the directions of threads on the surfaces of the two ends of the screw rod in the through groove are opposite, the two transmission blocks can drive the clamping blocks to move oppositely, and the size of a containing pipe containing hole in the sliding block can be adjusted; and placing pipes with different sizes can be conveniently mounted, so that the practicability of the device is higher.

Description

Technical Field

[0001] The utility model belongs to the technical field of DNA synthesizers, and in particular relates to a quantitative dispensing device for a DNA synthesizer. Background Art

[0002] Artificial DNA synthesis is the only known way to modify gene sequences in a targeted manner. It is widely used in many fields such as protein modification and life sciences, such as nucleic acid drugs, enzyme engineering, gene therapy, etc. The DNA synthesizer requires different steps such as deprotection, activation, capping, oxidation, etc. during the synthesis process. Different reagents need to be injected into the synthesis column during the synthesis process.

[0003] Chinese patent application No. 202120904902.3 discloses a quantitative dispensing device for a DNA synthesizer, which relates to the technical field of DNA synthesizers. The quantitative dispensing device for a DNA synthesizer includes a fixed frame, on which a dispensing mechanism and a placing mechanism are arranged, a pressure pump is fixedly installed on the top of the fixed frame, and a gas hose is fixedly connected to the output end of the pressure pump. The traditional dispensing method is to place the placement tube on a horizontal table, and then drip the DNA synthetic material into the placement tube one by one by a staff member holding a dropper. Compared with the traditional manual extrusion, on the one hand, the device can squeeze multiple copies of DNA synthetic materials at the same time, without the need for the staff member to hold a dropper and drip one by one into the placement tube. On the other hand, the purpose of controlling the dosage of the DNA synthetic material can be achieved by controlling the start time of the pressure pump. The device reduces the labor of the staff and saves the dispensing time, improves work efficiency, and is more accurate than the manual dripping of the DNA synthetic material.

[0004] In the above-mentioned public patent, the glass test tube is installed at the bottom of the dispensing glass tube through the sliding connection structure between the slider and the slide groove. Since the slider cannot be positioned, the sliding of the slider will affect the dispensing and cause leakage. The glass test tube and the slider are connected in a plug-in type structure. Since the size of the mounting hole inside the slider is fixed, the slider cannot be installed and placed in conjunction with test tubes of different sizes. Utility Model Content

[0005] In order to solve the problems raised in the above background technology, the utility model provides a quantitative dispensing device for a DNA synthesizer, which has the characteristics of good dispensing effect and high practicality.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a quantitative dispensing device for a DNA synthesizer, comprising a fixed frame, a concentrating box embedded in the interior of the fixed frame, a feed pipe connected to the top of the concentrating box, a pressure pump mechanism connected to the top of the concentrating box, a dispensing glass tube connected to the bottom of the concentrating box, a positioning component is arranged on the surface of the fixed frame, a slide groove is provided on the inner wall surface of the fixed frame, a slider is embedded in the interior of the slide groove, and an adjustment component is provided inside the slider.

[0007] Preferably, the positioning component includes an auxiliary component, a positioning groove, a positioning rod, a second spring, a side seat and a sleeve block, wherein a side seat is provided on the surface of the fixed frame, a positioning rod is embedded in the interior of the side seat, a sleeve block is sleeved on the surface of the positioning rod, a second spring is installed on the outer side of the sleeve block, a positioning groove is provided at the side end of the slider, an auxiliary component is installed on the side of the feed pipe, and a plug-in structure is provided between the positioning rod and the positioning groove.

[0008] Preferably, the auxiliary component includes a cover plate, a rotating part, a spring 1, a clamping block and a clamping slot, wherein a rotating part is installed on the side of the feed pipe, the side of the rotating part is connected to the cover plate, a spring 1 is embedded inside the cover plate, the side of the spring 1 is connected to the clamping block, a clamping slot is opened inside the feed pipe, and a plug-in structure is formed between the clamping block and the clamping slot.

[0009] Preferably, the clamping block and the inner groove of the cover plate are both L-shaped structures, and the surface of the clamping block fits tightly with the inner groove wall of the cover plate.

[0010] Preferably, the adjustment assembly includes a clamping block, a transmission block, a screw and a through slot, wherein a through slot is opened inside the slider, two transmission blocks are embedded inside the through slot, a clamping block is connected to the side of the transmission block, and a screw is embedded inside the transmission block.

[0011] Preferably, a positioning block is sleeved on the surface of the screw rod inside the slider, and the positioning block is in a circular ring structure, and the thread directions of the surfaces at both ends of the screw rod inside the through groove are opposite.

[0012] Compared with the prior art, the beneficial effects of the utility model are:

[0013] 1. The utility model sets a positioning component. Under the elastic action of the two pairs of sets of springs inside the side seat, the positioning rod and the positioning groove are tightly inserted to firmly position the placement tube directly below the dispensing glass tube, thereby making the dispensing of the device more accurate. After the movable card block is separated from the card slot, the cover plate can be rotated to open by the rotating part, which is more labor-saving and convenient to operate.

[0014] 2. The utility model sets an adjustment component, and the positioning block plays a positioning role. When the rotating screw is meshed with the transmission block, the threads on the surfaces at both ends of the screw inside the through groove are in opposite directions, so that the two transmission blocks can respectively drive the clamping blocks to move toward each other. The size of the placement hole of the placement tube inside the slider can be adjusted to facilitate the installation of placement tubes of different sizes, thereby making the device more practical. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is the front view of the utility model;

[0016] Figure 2 This is the front view of the positioning component of the utility model;

[0017] Figure 3 This is a front view of the auxiliary component of the utility model;

[0018] Figure 4 This is the front view of the adjustment component of the utility model.

[0019] In the figure: 1. feed pipe; 2. fixing frame; 3. dispensing glass tube; 4. slide groove; 5. slider; 6. adjustment assembly; 61. clamping block; 62. transmission block; 63. screw rod; 64. through groove; 65. positioning block; 7. positioning assembly; 71. auxiliary assembly; 711. cover plate; 712. rotating part; 713. spring 1; 714. clamping block; 715. clamping groove; 72. positioning groove; 73. positioning rod; 74. spring 2; 75. side seat; 76. sleeve block; 8. central box; 9. pressure pump mechanism. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0021] Example 1

[0022] See also Figure 1-4 The utility model provides the following technical solutions: a quantitative dispensing device for a DNA synthesizer, comprising a fixed frame 2, a concentrated box 8 is embedded in the fixed frame 2, a feeding pipe 1 is connected to the top of the concentrated box 8, a pressure pump mechanism 9 is connected to the top of the concentrated box 8, a dispensing glass tube 3 is connected to the bottom of the concentrated box 8, a positioning component 7 is arranged on the surface of the fixed frame 2, a slide groove 4 is opened on the inner wall surface of the fixed frame 2, a slider 5 is embedded in the slide groove 4, and an adjustment component 6 is opened in the slider 5.

[0023] Specifically, the positioning component 7 includes an auxiliary component 71, a positioning groove 72, a positioning rod 73, a second spring 74, a side seat 75 and a sleeve block 76. The surface of the fixed frame 2 is provided with a side seat 75, the interior of the side seat 75 is embedded with a positioning rod 73, the surface of the positioning rod 73 is sleeved with a sleeve block 76, the outer side of the sleeve block 76 is installed with a second spring 74, the side end of the slider 5 is provided with a positioning groove 72, the side of the feed pipe 1 is installed with an auxiliary component 71, and the positioning rod 73 and the positioning groove 72 are a plug-in structure.

[0024] By adopting the above technical solution, under the elastic action of the spring 74 inside the side seat 75 on the sleeve block 76, the tight insertion between the positioning rod 73 and the positioning groove 72 can make the placement tube firmly positioned directly below the dispensing glass tube 3, thereby making the dispensing of the device more accurate.

[0025] Specifically, the auxiliary component 71 includes a cover plate 711, a rotating member 712, a spring 713, a block 714 and a slot 715. The rotating member 712 is installed on the side of the feed pipe 1, the side of the rotating member 712 is connected to the cover plate 711, the inside of the cover plate 711 is embedded with a spring 713, the side of the spring 713 is connected to the block 714, the inside of the feed pipe 1 is provided with a slot 715, and the block 714 and the slot 715 are plug-in structures.

[0026] By adopting the above technical solution, after the movable block 714 is separated from the card slot 715, the cover 711 can be opened by rotating the rotating member 712, and then the DNA synthesis material is injected into the concentrated box 8 inside the fixed frame 2 through the feeding tube 1. Under the elastic force of the spring 1 713, the block 714 and the card slot 715 are engaged to close the cover 711 and position it. The cover 711 is easy to operate and has a better use effect.

[0027] Specifically, the clamping block 714 and the inner groove of the cover plate 711 are both L-shaped structures, and the surface of the clamping block 714 is tightly fitted with the inner groove wall of the cover plate 711 .

[0028] By adopting the above technical solution, the L-shaped card block 714 is easy to manually control the movement and the insertion or separation between the card slot 715, which makes the cover plate 711 more convenient to use.

[0029] When this embodiment is used, after the movable block 714 is separated from the card slot 715, the cover 711 can be rotated to open through the rotating member 712, and then the DNA synthetic material is injected into the concentrated box 8 inside the fixed frame 2 through the feeding tube 1. Under the elastic force of the spring 1 713, the block 714 and the card slot 715 are engaged to make the cover 711 cover and position. The switch operation of the cover 711 is simple and the use effect is better. The slider 5 slides into the slide groove 4 to drive the placement tube to be placed at the bottom of the dispensing glass tube 3. Under the elastic action of the spring 2 74 on the sleeve block 76 inside the side seat 75, the positioning rod 73 and the positioning groove 72 are tightly inserted to make the placement tube firmly positioned directly below the dispensing glass tube 3, thereby making the dispensing of the device more accurate. The pressure pump mechanism 9 is started to supply gas, and under the action of the air pressure, the DNA synthetic material can be dripped into each placement tube to complete the quantitative dispensing.

[0030] Example 2

[0031] The difference between this embodiment and embodiment 1 is that the adjustment component 6 includes a clamping block 61, a transmission block 62, a screw 63 and a through slot 64. The slider 5 is provided with a through slot 64. Two transmission blocks 62 are embedded in the through slot 64. The side of the transmission block 62 is connected to the clamping block 61. The transmission block 62 is embedded with a screw 63.

[0032] Specifically, a positioning block 65 is sleeved on the surface of the screw rod 63 inside the slider 5 , and the positioning block 65 is an annular structure. The threads of the surfaces at both ends of the screw rod 63 inside the through groove 64 are in opposite directions.

[0033] By adopting the above technical solution, the positioning block 65 plays a positioning role. When the rotating screw 63 is meshed with the transmission block 62, the thread directions of the two end surfaces of the screw 63 inside the through groove 64 are opposite, so that the two transmission blocks 62 can respectively drive the clamping blocks 61 to move toward each other. The size of the placement hole of the placement tube inside the slider 5 can be adjusted to facilitate the installation of placement tubes of different sizes, making the device more practical.

[0034] When this embodiment is used, the positioning block 65 plays a positioning role. When the rotating screw 63 is meshed with the transmission block 62, the thread directions of the two end surfaces of the screw 63 inside the through groove 64 are opposite, so that the two transmission blocks 62 can respectively drive the clamping blocks 61 to move toward each other. The size of the placement hole of the placement tube inside the slider 5 can be adjusted to facilitate the installation of placement tubes of different sizes, making the device more practical.

[0035] The structure and use principle of the feeding tube 1, the fixed frame 2, the dispensing glass tube 3, the slide groove 4, the slider 5, the adjustment assembly 6, the central box 8 and the pressure pump mechanism 9 in the utility model have been disclosed in Chinese patent application No. 202120904902.3, which discloses a quantitative dispensing device for a DNA synthesizer. Its working principle is to inject DNA synthetic materials into the central box 8 inside the fixed frame 2 through the feeding tube 1, so that the slider 5 slides into the slide groove 4 to drive the placement tube to be placed at the bottom of the dispensing glass tube 3, and the pressure pump mechanism 9 is started to supply gas. Under the action of air pressure, the DNA synthetic material can be dripped into each placement tube to complete the quantitative dispensing.

[0036] The working principle and use process of the utility model: when the utility model is used, after the movable card block 714 is separated from the card slot 715, the cover plate 711 can be rotated to open the cover plate 711 through the rotating member 712, and then the DNA synthesis material is injected into the concentrated box 8 inside the fixed frame 2 through the feeding tube 1, and under the elastic force of the spring 1 713, the card block 714 and the card slot 715 are engaged to make the cover plate 711 cover and position, the switch operation of the cover plate 711 is simple and the use effect is better, so that the slider 5 slides into the slide groove 4 to drive the placement tube to be placed at the bottom of the dispensing glass tube 3, and under the elastic action of the spring 2 74 on the sleeve block 76 inside the side seat 75, the positioning rod 73 and the positioning groove The tight insertion between 72 can make the placement tube firmly positioned directly below the dispensing glass tube 3, thereby making the dispensing of the device more accurate. The pressure pump mechanism 9 is started to supply gas. Under the action of air pressure, the DNA synthetic material can be dripped into each placement tube to complete quantitative dispensing. The positioning block 65 plays a positioning role. The rotating screw 63 is meshed with the transmission block 62. Since the thread directions of the two end surfaces of the screw 63 inside the through groove 64 are opposite, the two transmission blocks 62 can respectively drive the clamping blocks 61 to move toward each other. The size of the placement hole of the placement tube inside the slider 5 can be adjusted to facilitate the installation of placement tubes of different sizes, thereby making the device more practical.

[0037] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A quantitative dispensing device for a DNA synthesizer, comprising a fixing frame, characterized in that: A concentration box is embedded in the interior of the fixed frame, a feed pipe is connected to the top of the concentration box, a pressure pump mechanism is connected to the top of the concentration box, a dispensing glass tube is connected to the bottom of the concentration box, a positioning component is arranged on the surface of the fixed frame, a slide groove is provided on the inner wall surface of the fixed frame, a slider is embedded in the slide groove, and an adjustment component is provided inside the slider.

2. A quantitative dispensing device for a DNA synthesizer according to claim 1, characterized in that: The positioning component includes an auxiliary component, a positioning groove, a positioning rod, a second spring, a side seat and a sleeve block, wherein a side seat is arranged on the surface of the fixed frame, a positioning rod is embedded in the interior of the side seat, a sleeve block is sleeved on the surface of the positioning rod, a second spring is installed on the outer side of the sleeve block, a positioning groove is opened on the side end of the slider, an auxiliary component is installed on the side of the feed pipe, and a plug-in structure is formed between the positioning rod and the positioning groove.

3. The quantitative dispensing device for a DNA synthesizer according to claim 2, characterized in that: The auxiliary component includes a cover plate, a rotating part, a spring 1, a clamping block and a clamping slot, wherein a rotating part is installed on the side of the feed pipe, the side of the rotating part is connected to the cover plate, a spring 1 is embedded inside the cover plate, the side of the spring 1 is connected to the clamping block, a clamping slot is opened inside the feed pipe, and a plug-in structure is formed between the clamping block and the clamping slot.

4. The quantitative dispensing device for a DNA synthesizer according to claim 3, characterized in that: The inner groove of the clamping block and the cover plate are both L-shaped structures, and the surface of the clamping block is tightly fitted with the inner groove wall of the cover plate.

5. The quantitative dispensing device for a DNA synthesizer according to claim 1, characterized in that: The adjustment component includes a clamping block, a transmission block, a screw and a through slot, wherein the slider is provided with a through slot, two transmission blocks are embedded in the through slot, the side of the transmission block is connected to the clamping block, and the transmission block is embedded with a screw.

6. A quantitative dispensing device for a DNA synthesizer according to claim 5, characterized in that: The surface of the screw rod inside the slider is sleeved with a positioning block, which is in a circular ring structure. The threads of the surfaces at both ends of the screw rod inside the through groove are in opposite directions.

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