Constant-temperature maintaining device for macrophages

Abstract

The utility model relates to the technical field of macrophages, in particular to a macrophage constant-temperature maintaining device which comprises a box body, and a fixing plate is fixedly connected to the rear side of the bottom of an inner cavity of the box body. According to the macrophage constant-temperature maintaining device, a test tube filled with macrophages is placed in the inner cavity of the placing plate, and the servo motor drives the placing plate to rotate so as to drive the test tube to rotate, so that the test tube can be uniformly rotated and heated in the inner cavity of the water bath kettle body; the temperature of water in the water bath kettle body can be kept synchronous up and down under the action of meshing connection of teeth of the driving gear and the driven gear while stirring, so that the survival rate and the biological activity of macrophages are improved, and the problem that the water is slowly heated from the lower part in a general water bath heating process and the heating efficiency is low is solved. The phenomenon that water at the lower part firstly reaches the temperature but water at the upper part does not reach the temperature is possibly caused, so that the survival rate, the biological activity and the like of macrophages are possibly influenced.

Description

Technical Field

[0001] The utility model relates to the technical field of macrophages, in particular to a macrophage constant temperature maintaining device. Background Art

[0002] Macrophages are a type of white blood cell located in tissues. They originate from monocytes, which in turn originate from precursor cells in the bone marrow. Both macrophages and monocytes are phagocytes, and participate in nonspecific defense (innate immunity) and specific defense (cellular immunity) in vertebrates. Their main function is to phagocytose (i.e., engulf and digest) cell fragments and pathogens in the form of fixed cells or free cells, and activate lymphocytes or other immune cells to respond to pathogens. Macrophages are immune cells with multiple functions and are an important subject for studying cellular phagocytosis, cellular immunity, and molecular immunology. Macrophages are easy to obtain, easy to culture, and can be purified. Macrophages are a non-reproductive cell group that can live for 2-3 weeks under suitable conditions. They are mostly used for primary culture and are difficult to survive for a long time.

[0003] Macrophage culture is a very important experimental method in modern biomedical scientific research. The process of macrophage culture is usually carried out in a constant temperature environment. Therefore, a constant temperature maintenance device is required. Most of the existing constant temperature maintenance devices for macrophage culture use water bath heating to maintain a constant temperature state. However, when the water bath is heated for macrophage culture, the test tube is fixed in the water bath pot. Usually, the water is heated from the bottom and the water is heated slowly. This may cause the water at the bottom to reach the temperature first while the water at the top does not reach the temperature, which may affect the survival rate and biological activity of the macrophages.

[0004] Therefore, those skilled in the art provide a macrophage constant temperature maintenance device to solve the problems raised in the above background technology. Utility Model Content

[0005] The technical problem to be solved by the utility model is that in the prior art, there is a disadvantage that the water bath heating process is usually carried out from the bottom and the water is heated slowly, which may cause the water at the bottom to reach the temperature first while the water at the top has not reached the temperature, which may affect the survival rate and biological activity of macrophages. For this reason, we propose a macrophage constant temperature maintenance device.

[0006] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0007] A macrophage constant temperature maintenance device comprises a box body, a fixing plate is fixedly connected to the rear side of the bottom of the inner cavity of the box body, a cylinder is fixedly connected to the top of the fixing plate, a mounting seat is fixedly connected to the top of the cylinder, a constant temperature mechanism is arranged in the inner cavity of the box body, and the constant temperature mechanism comprises a servo motor;

[0008] The servo motor is fixedly connected to the top of the mounting seat, the output end of the servo motor is fixedly connected to a stirring shaft, the bottom of the stirring shaft is fixedly connected to a placement plate, the top of the placement plate is provided with a notch, the middle end of the bottom of the inner cavity of the box body is fixedly connected to a rotating rod, the top of the rotating rod is fixedly connected to a turntable, the top of the turntable is fixedly connected to a water bath body, the bottom of the inner cavity of the box body and the right side of the turntable are fixedly connected to a stepper motor, the output end of the stepper motor is fixedly connected to a rotating shaft, the outer surface of the rotating shaft is fixedly connected to a driving gear, the outer surface of the turntable is fixedly connected to a driven gear, and the driving gear is meshingly connected to the driven gear.

[0009] As a further solution of the utility model: fixing blocks are fixedly connected to the four sides of the inner wall of the water bath body, and the inner surface of the fixing block is fixedly connected to the heating tube.

[0010] As a further solution of the utility model: a touch device is fixedly arranged on the right side of the box body, and a box door is movably connected to the front side of the box body through a hinge.

[0011] As a further solution of the utility model: an observation window is fixedly connected to the outer surface of the box door, and a handle is fixedly connected to the outer surface of the box door and located below the observation window.

[0012] As a further solution of the utility model: ultraviolet lamp tubes are fixedly connected to the left and right sides of the inner cavity of the box body, and ultraviolet lamp covers are arranged on the outer sides of the ultraviolet lamp tubes.

[0013] As a further solution of the utility model: a protective cover is arranged on the outer side of the servo motor, and the protective cover is connected to the inner surface of the top of the mounting seat through threads.

[0014] As a further solution of the utility model: support legs are fixedly connected to the four sides of the bottom of the box body, and anti-slip pads are fixedly connected to the bottoms of the support legs.

[0015] The beneficial effects of the utility model are:

[0016] 1. The utility model places a test tube containing macrophages in the inner cavity of a placement plate, drives the placement plate to rotate through a servo motor, thereby driving the test tube to rotate, so that the test tube can rotate evenly in the inner cavity of a water bath body and be heated. While stirring, the water bath body is driven to rotate in the opposite direction through the meshing connection of the driving gear and the driven gear, thereby accelerating the exchange flow between the upper and lower water layers, so that the temperature of the water in the water bath body can be kept synchronized between the upper and lower water layers, and the influence of uneven water temperature on the macrophages in the test tube is reduced. When the temperature is lowered, the heating tube is controlled by a touch device to perform heating work, so that the water inside the water bath body is kept at a constant temperature, thereby improving the survival rate and biological activity of the macrophages.

[0017] 2. The utility model can heat the water in the inner cavity of the water bath body to a suitable temperature by arranging a heating tube. By arranging a touch device, it is convenient for the operator to understand the operating status of the equipment through the touch device, and can control the entire equipment through the touch device. By arranging an observation window, it is convenient for the operator to observe the interior of the box through the observation window. By arranging an ultraviolet lamp, the interior of the box can be disinfected and sterilized. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a structural schematic diagram of a macrophage constant temperature maintenance device;

[0019] Figure 2 This is a schematic diagram of the cross-sectional structure of a box in a macrophage constant temperature maintenance device;

[0020] Figure 3 It is a schematic diagram of the structure of a constant temperature mechanism in a macrophage constant temperature maintenance device;

[0021] Figure 4 It is a schematic diagram of the structure of a macrophage constant temperature maintenance device in a disassembled protective cover;

[0022] Figure 5 The present invention is a schematic diagram of the disassembled structure of the ultraviolet lamp in a macrophage constant temperature maintenance device. In the figure: 1. Box; 2. Constant temperature mechanism; 201. Servo motor; 202. Stirring shaft; 203. Placement plate; 204. Rotating rod; 205. Turntable; 206. Water bath body; 207. Stepping motor; 208. Rotating shaft; 209. Driving gear; 210. Driven gear; 211. Fixed block; 212. Heating tube; 3. Fixed plate; 4. Cylinder; 5. Mounting seat; 6. Touch device; 7. Box door; 8. Observation window; 9. Handle; 10. Ultraviolet lamp; 11. Ultraviolet lamp cover; 12. Protective cover; 13. Support leg; 14. Anti-slip pad. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] See also Figure 1-5 A macrophage constant temperature maintenance device comprises a box body 1, a fixing plate 3 is fixedly connected to the rear side of the bottom of the inner cavity of the box body 1, a cylinder 4 is fixedly connected to the top of the fixing plate 3, a mounting seat 5 is fixedly connected to the top of the cylinder 4, a constant temperature mechanism 2 is arranged in the inner cavity of the box body 1, and the constant temperature mechanism 2 comprises a servo motor 201;

[0025] The servo motor 201 is fixedly connected to the top of the mounting base 5, the output end of the servo motor 201 is fixedly connected to the stirring shaft 202, the bottom of the stirring shaft 202 is fixedly connected to the placement plate 203, the top of the placement plate 203 is provided with a slot, the middle end of the bottom of the inner cavity of the box body 1 is fixedly connected to a rotating rod 204, the top of the rotating rod 204 is fixedly connected to a turntable 205, the top of the turntable 205 is fixedly connected to a water bath pot body 206, the bottom of the inner cavity of the box body 1 and on the right side of the turntable 205 is fixedly connected to a stepper motor 207, the output end of the stepper motor 207 is fixedly connected to a rotating shaft 208, the outer surface of the rotating shaft 208 is fixedly connected to a driving gear 209, the outer surface of the turntable 205 is fixedly connected to a driven gear 210, and the driving gear 209 is meshingly connected with the driven gear 210.

[0026] Through the above technical solution, by placing the test tube containing macrophages in the inner cavity of the placement plate 203, the servo motor 201 drives the placement plate 203 to rotate, thereby driving the rotation of the test tube, so that the test tube can rotate and heat evenly in the inner cavity of the water bath body 206, and while stirring, the teeth of the driving gear 209 and the driven gear 210 are engaged and connected to drive the water bath body 206 to rotate in the opposite direction, accelerate the exchange flow between the upper and lower water layers, so that the temperature of the water in the water bath body 206 can be kept synchronized, and the influence of uneven water temperature on the macrophages in the test tube is reduced. When the temperature is lowered, the heating tube 212 is controlled by the touch device 6 to perform heating work, so that the water inside the water bath body 206 is kept at a constant temperature, thereby improving the survival rate and biological activity of the macrophages.

[0027] Specifically, the inner wall of the water bath body 206 is fixedly connected with fixing blocks 211 all around, and the inner surface of the fixing blocks 211 is fixedly connected with the heating tube 212 .

[0028] Through the above technical solution, by providing the heating tube 212, the water in the inner cavity of the water bath body 206 can be heated to a suitable temperature.

[0029] Specifically, a touch device 6 is fixedly provided on the right side of the box body 1, and a box door 7 is movably connected to the front side of the box body 1 through a hinge.

[0030] Through the above technical solution, by setting the touch device 6, it is convenient for the operator to understand the operating status of the equipment through the touch device 6, and the entire equipment can be controlled through the touch device 6.

[0031] Specifically, an observation window 8 is fixedly connected to the outer surface of the box door 7 , and a handle 9 is fixedly connected to the outer surface of the box door 7 and located below the observation window 8 .

[0032] Through the above technical solution, by providing the observation window 8, it is convenient for the operator to observe the interior of the box body 1 through the observation window 8.

[0033] Specifically, the left and right sides of the inner cavity of the box body 1 are fixedly connected with the ultraviolet lamp tube 10 , and the outer side of the ultraviolet lamp tube 10 is provided with an ultraviolet lamp cover 11 .

[0034] Through the above technical solution, by providing the ultraviolet lamp 10, the interior of the box 1 can be disinfected and sterilized.

[0035] Specifically, a protective cover 12 is disposed on the outer side of the servo motor 201 , and the protective cover 12 is connected to the inner surface of the top of the mounting seat 5 through threads.

[0036] Through the above technical solution, by providing the protective cover 12, the servo motor 201 can be protected.

[0037] Specifically, the four sides of the bottom of the box body 1 are fixedly connected with support legs 13 , and the bottoms of the support legs 13 are fixedly connected with anti-slip pads 14 .

[0038] Through the above technical solution, by providing the anti-skid pad 14, an anti-skid effect can be achieved and the overall stability can be improved.

[0039] The working principle of the utility model is: a macrophage constant temperature maintenance device, firstly, a test tube containing macrophages is placed in the inner cavity of the placement plate 203, and the water in the inner cavity of the water bath body 206 is heated by the heating tube 212, and then the mounting seat 5 is driven downward by the cylinder 4 to extend the stirring shaft 202 and the placement plate 203 to extend into the inner cavity of the water bath body 206, and then the stirring shaft 202 is driven to rotate by the output end of the servo motor 201, and the stirring shaft 202 rotates while cooperating with the placement plate 203 to rotate synchronously, so that the test tube in the inner cavity of the placement plate 203 can rotate evenly inside the water bath body 206 and be heated, and the water in the inner cavity of the water bath body 206 can be stirred while rotating, and the stepping motor 207 is stirring while stirring. The output end drives the rotating shaft 208 to rotate, and the rotating shaft 208 rotates while cooperating with the driving gear 209 to rotate synchronously. The meshing connection between the driving gear 209 and the driven gear 210 drives the turntable 205 to rotate. During the rotation process, the turntable 205 drives the water bath body 206 and the placement plate 203 to rotate in opposite directions, thereby accelerating the exchange flow between the upper and lower water layers, so that the temperature of the water in the water bath body 206 can be kept synchronized with the upper and lower water layers, reducing the impact of uneven water temperature on the macrophages in the test tube. When the water temperature in the inner cavity of the water bath body 206 is too low, the heating tube 212 is controlled by the touch device 6 to perform heating work, so that the water inside the water bath body 206 is kept at a constant temperature, thereby improving the survival rate and biological activity of the macrophages.

[0040] What is described above is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A macrophage constant temperature maintenance device, comprising a housing (1), characterized in that: A fixing plate (3) is fixedly connected to the rear side of the bottom of the inner cavity of the box body (1), a cylinder (4) is fixedly connected to the top of the fixing plate (3), a mounting seat (5) is fixedly connected to the top of the cylinder (4), and a constant temperature mechanism (2) is arranged in the inner cavity of the box body (1), and the constant temperature mechanism (2) includes a servo motor (201); The servo motor (201) is fixedly connected to the top of the mounting seat (5); the output end of the servo motor (201) is fixedly connected to a stirring shaft (202); the bottom of the stirring shaft (202) is fixedly connected to a placement plate (203); a notch is provided on the top of the placement plate (203); a rotating rod (204) is fixedly connected to the middle end of the bottom of the inner cavity of the box body (1); the top of the rotating rod (204) is fixedly connected to a rotating disk (205); the top of the rotating disk (205) is A water bath body (206) is fixedly connected thereto; a stepper motor (207) is fixedly connected to the bottom of the inner cavity of the housing (1) and located on the right side of the turntable (205); a rotating shaft (208) is fixedly connected to the output end of the stepper motor (207); a driving gear (209) is fixedly connected to the outer surface of the rotating shaft (208); a driven gear (210) is fixedly connected to the outer surface of the turntable (205); and the driving gear (209) is meshingly connected to the driven gear (210).

2. A macrophage constant temperature maintenance device according to claim 1, characterized in that: The inner wall of the water bath body (206) is fixedly connected to fixing blocks (211) on all sides, and the inner surface of the fixing blocks (211) is fixedly connected to a heating tube (212).

3. A macrophage constant temperature maintenance device according to claim 1, characterized in that: A touch device (6) is fixedly arranged on the right side of the box body (1), and a box door (7) is movably connected to the front side of the box body (1) via a hinge.

4. A macrophage constant temperature maintenance device according to claim 3, characterized in that: An observation window (8) is fixedly connected to the outer surface of the box door (7), and a handle (9) is fixedly connected to the outer surface of the box door (7) and located below the observation window (8).

5. The macrophage constant temperature maintenance device according to claim 1, characterized in that: Ultraviolet lamp tubes (10) are fixedly connected to the left and right sides of the inner cavity of the box body (1), and an ultraviolet lamp cover (11) is arranged on the outer side of the ultraviolet lamp tube (10).

6. The macrophage constant temperature maintenance device according to claim 1, characterized in that: A protective cover (12) is provided on the outside of the servo motor (201), and the protective cover (12) is connected to the inner surface of the top of the mounting seat (5) via threads.

7. A macrophage constant temperature maintenance device according to claim 1, characterized in that: Support legs (13) are fixedly connected to the four sides of the bottom of the box body (1), and anti-slip pads (14) are fixedly connected to the bottoms of the support legs (13).

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