Disposable 3D sterile biological reaction bag for high-density adherent cell culture

Abstract

The utility model discloses a disposable three-dimensional (3D) sterile biological reaction bag for high-density adherent cell culture, which comprises a disposable 3D bag body, a first liquid inlet is arranged on the front side or the rear side of the disposable 3D bag body, an interception membrane is arranged on one side of the first liquid inlet on the inner surface of the disposable 3D bag body, and a second liquid inlet is arranged on the other side of the first liquid inlet on the inner surface of the disposable 3D bag body. A plastic filter screen is arranged between the interception film and the disposable 3D bag body and used for preventing the interception film from being attached to the disposable 3D bag body. The disposable 3D sterile biological reaction bag has the advantages that the microcarrier interception membrane is arranged in the disposable 3D sterile biological reaction bag, and the microcarrier interception membrane and the disposable 3D sterile biological reaction bag form a whole, so that the separation of a microcarrier and a culture medium is facilitated, and meanwhile, the risk that the cell culture medium is polluted and the risk that the microcarrier blocks a pipeline can be greatly reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of high-density adherent cell culture, in particular to a disposable 3D sterile bioreactor bag for high-density adherent cell culture. Background Art

[0002] At present, high-density adherent cells are usually cultured by combining high-concentration microcarriers with perfusion culture technology. In order to effectively carry out perfusion culture, microcarriers need to be trapped. Commonly used trapping techniques mainly include filtration, centrifugation, sedimentation, etc. In practical applications, these technologies or devices are often unsatisfactory due to limited efficiency, high cost, easy contamination, etc.

[0003] Chinese patent application CN202010750200.4 provides a new type of high-density microcarrier retention device for perfusion culture and a method of use. In this device, the microcarrier retention device is an external design and is connected to the bioreactor through a pipe. Although this new design of the device is highly efficient in promoting the separation of microcarriers and cell culture media, it helps the perfusion culture of adherent cells and microcarriers, and can make the culture volume in the bioreactor more flexible. However, there are also risks of easy contamination and clogging, such as: microcarriers will remain in the pipeline, especially at the corners and joints of the pipeline, causing pipeline blockage; pipeline connection, need to be connected separately, there is a risk of contamination during the connection operation. If the connection part is not tightly sealed, on the one hand, liquid leakage will occur, causing environmental pollution; on the other hand, external air will enter the pipeline and contaminate the culture fluid in the pipeline. Utility Model Content

[0004] The utility model aims to provide a disposable 3D sterile bioreactor bag for high-density adherent cell culture, so as to solve the technical problems existing in the background technology.

[0005] In order to achieve the above purpose, the technical solution adopted by the utility model is as follows:

[0006] A disposable 3D sterile bioreactor bag for high-density adherent cell culture comprises a disposable 3D bag body, a first liquid inlet is arranged on the front side or the rear side of the disposable 3D bag body, a retention membrane is arranged on the side of the first liquid inlet located inside the disposable 3D bag body, a plastic filter is arranged between the retention membrane and the disposable 3D bag body, and the plastic filter is used to prevent the retention membrane from fitting to the disposable 3D bag body.

[0007] Furthermore, a first liquid inlet pipeline is connected to a side of the first liquid inlet located outside the disposable 3D bag body.

[0008] Furthermore, a second liquid inlet, a third liquid inlet, a fourth liquid inlet, a fifth liquid inlet, an air outlet and a pressure measuring port are also provided on the disposable 3D bag body, and a second liquid inlet pipeline, a third liquid inlet pipeline, a fourth liquid inlet pipeline, a fifth liquid inlet pipeline, an air outlet pipeline and a pressure measuring port are sequentially connected to the second liquid inlet, the third liquid inlet, the fourth liquid inlet, the fifth liquid inlet, the air outlet and the pressure measuring port on the side outside the disposable 3D bag body.

[0009] Furthermore, a sampling and liquid outlet port, a first pH value measuring port and a first DO value measuring port are also provided on the disposable 3D bag body, and a sampling and liquid outlet pipeline, a first pH value measuring pipeline and a first DO value measuring pipeline are sequentially connected to the sampling and liquid outlet port, the first pH value measuring port and the first DO value measuring port on the side outside the disposable 3D bag body.

[0010] Furthermore, a second pH value measuring port, a second DO value measuring port and a temperature measuring port are also provided on the disposable 3D bag body, and a second pH value measuring pipeline, a second DO value measuring pipeline and a temperature measuring pipeline are connected in sequence on the side of the second pH value measuring port, the second DO value measuring port and the temperature measuring port located outside the disposable 3D bag body.

[0011] Furthermore, a stirring paddle kit is provided on the bottom side of the disposable 3D bag body, and the stirring paddle kit is used to install a stirring paddle. Two first air inlets, two second air inlets and two first liquid outlets are opened on the stirring paddle kit, and the two air inlets are commonly connected to a first air inlet pipeline, the two second air inlets are commonly connected to a second air inlet pipeline, and the two first liquid outlets are commonly connected to a first liquid outlet pipeline.

[0012] Furthermore, the disposable 3D bag body is formed by an upper bag piece, a lower bag piece, a front bag piece and a rear bag piece.

[0013] Compared with the prior art, the utility model has the following advantages: the microcarrier retention membrane is arranged inside the disposable 3D sterile bioreactor bag, and forms a whole with the disposable 3D sterile bioreactor bag, which has a simple structure and is easy to operate. It not only helps to separate the microcarrier and the cell culture medium, but also greatly reduces the risk of cell culture medium contamination and the risk of microcarrier clogging the pipeline. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the technical solution in this embodiment, the following is a brief introduction to the drawings required for use in the description of the embodiment. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0015] Figure 1It is a front axonometric schematic diagram of the first embodiment of the utility model;

[0016] Figure 2 It is a rear axonometric schematic diagram of the first embodiment of the utility model;

[0017] Figure 3 It is a bottom view of the first embodiment of the utility model;

[0018] Figure 4 It is a cross-sectional view of the first embodiment of the utility model;

[0019] Figure 5 It is a cross-sectional view of a second embodiment of the utility model;

[0020] Figure 6 It is a front axonometric schematic diagram of a third embodiment of the utility model;

[0021] Figure 7 It is a cross-sectional view of a third embodiment of the utility model;

[0022] Figure 8 is a cross-sectional view of a fourth embodiment of the utility model;

[0023] Fig. 9 It is a specific embodiment of the first liquid inlet pipeline;

[0024] Fig.10 It is a specific embodiment of the second liquid inlet pipeline;

[0025] Fig.11 It is a specific embodiment of the third liquid inlet pipeline;

[0026] Fig.12 It is a specific embodiment of the fourth liquid inlet pipeline;

[0027] Fig.13 It is a specific embodiment of the fifth liquid inlet pipeline;

[0028] Fig.14 It is a specific embodiment of the gas outlet pipeline;

[0029] Fig.15 It is a specific embodiment of a pressure measuring pipeline;

[0030] Fig.16 It is a specific embodiment of a sampling and liquid outlet pipeline;

[0031] Fig.17 This is a specific embodiment of a first pH value measuring pipeline;

[0032] Fig.18 It is a specific embodiment of the second pH value measuring pipeline;

[0033] Fig.19This is a specific embodiment of a first DO value measuring pipeline;

[0034] Fig. 20 This is a specific embodiment of a second DO value measuring pipeline;

[0035] Fig.21 It is a specific embodiment of a temperature measuring pipeline;

[0036] Fig. 22 is a specific embodiment of the first air intake pipeline or the second air intake pipeline;

[0037] Fig.23 It is a specific embodiment of the first liquid outlet pipeline;

[0038] Description of reference numerals: 1. disposable 3D bag body; 1a. upper bag sheet; 1b. lower bag sheet; 1c. front bag sheet; 1d. rear bag sheet; 2. first liquid inlet; 3. interception membrane; 4. plastic filter; 5. second liquid inlet; 6. third liquid inlet; 7. fourth liquid inlet; 8. fifth liquid inlet; 9. air outlet; 10. pressure measuring port; 11. sampling and liquid outlet; 12. first pH value measuring port; 13. first DO value measuring port; 14. second pH value measuring port; 15. second DO value measuring port; 16. temperature measuring port; 17. stirring paddle kit; 17a. first air inlet; 17b. second air inlet; 17c. first liquid outlet; 18. first liquid inlet pipeline; 18a. single joint A; 18b. thermoplastic tube A; 18c. Reducer A; 18d, thermoplastic tube B; 18e, equal-diameter joint A; 18f, peristaltic pump tube A; 18g, equal-diameter joint B; 18h, thermoplastic tube C; 18i, plug A; 19, second liquid inlet pipeline; 19a, single joint B; 19b, thermoplastic tube D; 19c, reducer B; 19d, thermoplastic tube E; 19e, Y-type three-way joint A; 19f, thermoplastic tube F; 19g, equal-diameter joint C; 19h, peristaltic pump tube B; 19i, equal-diameter joint D; 19j, thermoplastic tube G; 19k, plug B; 20, third liquid inlet pipeline; 20a, single joint C; 20b, thermoplastic tube H; 20c, reducer C; 20d, thermoplastic tube I; 20e, plug C; 21, fourth liquid inlet pipeline; 21a, single joint D; 21b, thermoplastic tube J; 21c, reducer D; 21d, thermoplastic tube K; 21e, equal-diameter joint E; 21f, peristaltic pump tube C; 21g, equal-diameter joint F; 21h, thermoplastic tube L; 21i, plug D; 22, fifth liquid inlet pipeline; 22a, single joint E; 22b, thermoplastic tube M; 22c, reducer E; 22d, thermoplastic tube N; 22e, equal-diameter joint G; 22f, peristaltic pump tube D; 22g, equal-diameter joint H; 22h, thermoplastic tube O; 22i, plug E; 23, air outlet pipeline; 23a, single joint F; 23b, thermoplastic tube P; 23c, T-type three-way joint A; 23d, thermoplastic tube Q; 23e, thermoplastic tube R; 23f, air filter A; 23g, plug F; 24 , pressure measuring pipeline; 24a, single joint G; 24b, thermoplastic tube S; 24c, pressure sensor; 24d, thermoplastic tube T; 24e, air filter B; 25, sampling and liquid outlet pipeline; 25a, single joint H; 25b, thermoplastic tube U; 25c, reducer F; 25d, thermoplastic tube V; 25e, T-type three-way connector B; 25f, thermoplastic tube W; 25g, thermoplastic tube X; 25h, female Luer connector; 25i, needleless sampling connector; 25j, coreless Luer cap; 25k, ziplock bag A; 25m, plug G; 26, the first pH value measuring pipeline; 26a, single joint I; 26b, medical silicone tube A; 26c, sterile connector female A; 26d, ziplock bag B; 27, the first DO value measuring pipeline;27a, single connector J; 27b, medical silicone tube B; 27c, sterile connector female B; 27d, ziplock bag C; 28, second pH value measurement pipeline; 28a, single connector K; 28b, medical silicone tube C; 28c, sterile connector female C; 28d, ziplock bag D; 29, second DO value measurement pipeline; 29a, single connector L; 29b, medical silicone tube D; 29c, sterile connector female D; 29d, ziplock bag E; 30, temperature measurement pipeline; 30a, single connector M; 30b, medical silicone tube E; 30c, double connector; 30d, thermoplastic tube Y; 30 e, pearlescent cable tie; 31, first air inlet pipeline; 31a, thermoplastic tube Z; 31b, Y-type three-way joint B; 31c, thermoplastic tube II; 31d, reducer G; 31e, thermoplastic tube III; 31f, air filter C; 32, second air inlet pipeline; 33, first liquid outlet pipeline; 33a, thermoplastic tube IV; 33b, Y-type three-way joint C; 33c, thermoplastic tube V; 33d, equal diameter joint I; 33e, peristaltic pump tube E; 33f, equal diameter joint J; 33g, thermoplastic tube VI; 33h, sterile connector; 33i, ziplock bag F; 34, adapter; 35, connecting pipe. ; DETAILED DESCRIPTION

[0039] In order to make the purpose, technical solution and advantages of the utility model clearer, the technical solution in the embodiment of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiment of the utility model. Obviously, the described embodiment is a part of the embodiment of the utility model, not all of the embodiments. The components of the embodiment of the utility model described and shown in the drawings here can be arranged and designed in various different configurations.

[0040] In the description of the embodiments of the present utility model, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0041] The utility model is further described below in conjunction with the accompanying drawings:

[0042] like Figures 1 to 4As shown, the first embodiment of the disposable 3D sterile bioreactor bag for high-density adherent cell culture provided by the utility model comprises a disposable 3D bag body 1, a first liquid inlet 2 is arranged on the front side of the disposable 3D bag body 1, a retention membrane 3 is arranged on one side of the first liquid inlet 2 located on the inner surface of the disposable 3D bag body 1, a plastic filter net 4 is arranged between the retention membrane 3 and the disposable 3D bag body 1, the plastic filter net 4 is used to prevent the retention membrane 3 from being completely attached to the disposable 3D bag body 1 and hindering the liquid inlet, and the retention membrane 3 is used to retain microcarriers.

[0043] Specifically, in this embodiment, a first liquid inlet pipeline 18 is connected to a side of the first liquid inlet 2 located outside the disposable 3D bag body 1 .

[0044] Specifically, as a preferred example of the first liquid inlet pipeline 18: Fig. 9 As shown, it specifically includes a single joint A18a, a thermoplastic tube A18b, a reducing joint A18c, a thermoplastic tube B18d, an equal-diameter joint A18e, a peristaltic pump tube A18f, an equal-diameter joint B18g, a thermoplastic tube C18h and a plug A18i; wherein, one end of the thermoplastic tube A18b is connected to the first liquid inlet 2 through the single joint A18a, and the other end is connected to one end of the thermoplastic tube B18d through the reducing joint A18c, the other end of the thermoplastic tube B18d is connected to one end of the peristaltic pump tube A18f through the equal-diameter joint A18e, the other end of the peristaltic pump tube A18f is connected to one end of the thermoplastic tube C18h through the equal-diameter joint B18g, and the other end of the thermoplastic tube C18h is connected to the plug A18i.

[0045] Specifically, in this embodiment, a second liquid inlet 5, a third liquid inlet 6, a fourth liquid inlet 7, a fifth liquid inlet 8, an air outlet 9 and a pressure measuring port 10 are arranged on the top side of the disposable 3D bag body 1, and a second liquid inlet pipeline 19, a third liquid inlet pipeline 20, a fourth liquid inlet pipeline 21, a fifth liquid inlet pipeline 22, an air outlet pipeline 23 and a pressure measuring pipeline 24 are sequentially connected to the second liquid inlet 5, the third liquid inlet 6, the fourth liquid inlet 7, the fifth liquid inlet 8, the air outlet 9 and the pressure measuring port 10 on the side outside the disposable 3D bag body 1.

[0046] Specifically, as a preferred example of the second liquid inlet pipeline 19: Fig.10As shown, it specifically includes a single joint B19a, a thermoplastic tube D19b, a reducer B19c, a thermoplastic tube E19d, a Y-type three-way joint A19e, two thermoplastic tubes F19f, two equal-diameter joints C19g, two peristaltic pump tubes B19h, two equal-diameter joints D19i, two thermoplastic tubes G19j and two plugs B19k; wherein one end of the thermoplastic tube D19b is connected to the second liquid inlet 5 through the single joint B19a, and the other end is connected to the second liquid inlet 5 through the reducer B19a. 9c is connected to one end of a thermoplastic tube E19d, the other end of the thermoplastic tube E19d is connected to one end of two thermoplastic tubes F19f through a Y-type three-way connector A19e, the other end of the two thermoplastic tubes F19f is connected to one end of two peristaltic pump tubes B19h through two equal-diameter connectors C19g, the other end of the two peristaltic pump tubes B19h is connected to one end of two thermoplastic tubes G19j through two equal-diameter connectors D19i, and the other end of the two thermoplastic tubes G19j is connected to two plugs B19k.

[0047] Specifically, as a preferred example of the third liquid inlet pipeline 20: Fig.11 As shown, it specifically includes a single joint C20a, a thermoplastic tube H20b, a reducer C20c, a thermoplastic tube I20d and a plug C20e; wherein, one end of the thermoplastic tube H20b is connected to the third liquid inlet 6 through the single joint C20a, and the other end is connected to one end of the thermoplastic tube I20d through the reducer C20c, and the other end of the thermoplastic tube I20d is connected to the plug C20e.

[0048] Specifically, as a preferred example of the fourth liquid inlet pipeline 21: Fig.12 As shown, it specifically includes a single joint D21a, a thermoplastic tube J21b, a reducer D21c, a thermoplastic tube K21d, an equal-diameter joint E21e, a peristaltic pump tube C21f, an equal-diameter joint F21g, a thermoplastic tube L21h and a plug D21i; wherein, one end of the thermoplastic tube J21b is connected to the fourth liquid inlet 7 through the single joint D21a, and the other end is connected to one end of the thermoplastic tube K21d through the reducer D21c, the other end of the thermoplastic tube K21d is connected to one end of the peristaltic pump tube C21f through the equal-diameter joint E21e, the other end of the peristaltic pump tube C21f is connected to one end of the thermoplastic tube L21h through the equal-diameter joint F21g, and the other end of the thermoplastic tube L21h is connected to the plug D21i.

[0049] Specifically, as a preferred example of the fifth liquid inlet pipeline 22: Fig.13As shown, it specifically includes a single joint E22a, a thermoplastic tube M22b, a reducing joint E22c, a thermoplastic tube N22d, an equal-diameter joint G22e, a peristaltic pump tube D22f, an equal-diameter joint H22g, a thermoplastic tube O22h and a plug E22i; wherein, one end of the thermoplastic tube M22b is connected to the fifth liquid inlet 8 through the single joint E22a, and the other end is connected to one end of the thermoplastic tube N22d through the reducing joint E22c, the other end of the thermoplastic tube N22d is connected to one end of the peristaltic pump tube D22f through the equal-diameter joint G22e, the other end of the peristaltic pump tube D22f is connected to one end of the thermoplastic tube O22h through the equal-diameter joint H22g, and the other end of the thermoplastic tube O22h is connected to the plug E22i.

[0050] Specifically, as a preferred example of the air outlet pipeline 23: Fig.14 As shown, it specifically includes a single joint F23a, a thermoplastic tube P23b, a T-type three-way joint A23c, a thermoplastic tube Q23d, a thermoplastic tube R23e, an air filter A23f and a plug F23g; wherein, one end of the thermoplastic tube P23b is connected to the air outlet 9 through the single joint F23a, and the other end is connected to the thermoplastic tube Q23d and one end of the thermoplastic tube R23e through the T-type three-way joint A23c, the other end of the thermoplastic tube Q23d is connected to the air filter A23f, and the other end of the thermoplastic tube R23e is connected to the plug F23g.

[0051] Specifically, as a preferred example of the pressure measuring pipeline 24: Fig.15 As shown, it specifically includes a single joint G24a, a thermoplastic tube S24b, a pressure sensor 24c, a thermoplastic tube T24d and an air filter B24e; wherein, one end of the thermoplastic tube S24b is connected to the pressure measuring port 10 through the single joint G24a, and the other end is connected to the pressure sensor 24c, and the pressure sensor 24c is connected to the air filter B24e through the thermoplastic tube T24d.

[0052] Specifically, in this embodiment, a sampling and liquid outlet port 11, a first pH value measuring port 12 and a first DO value measuring port 13 are also provided on the front side of the disposable 3D bag body 1, and a sampling and liquid outlet pipeline 25, a first pH value measuring pipeline 26 and a first DO value measuring pipeline 27 are sequentially connected to the sampling and liquid outlet port 11, the first pH value measuring port 12 and the first DO value measuring port 13 on the side outside the disposable 3D bag body 1.

[0053] Specifically, as a preferred example of the sampling and liquid outlet pipeline 25: Fig.16As shown, it specifically includes a single connector H25a, a thermoplastic tube U25b, a reducer F25c, a thermoplastic tube V25d, a T-type three-way connector B25e, a thermoplastic tube W25f, a thermoplastic tube X25g, a female Luer connector 25h, a needleless sampling connector 25i, a coreless Luer protective cap 25j, a ziplock bag A25k and a plug G25m; wherein one end of the thermoplastic tube U25b is connected to the sampling and liquid outlet 11 through the single connector F25a, and the other end is connected to the thermoplastic tube U25b through the reducer F25c. One end of the thermoplastic tube V25d is connected, and the other end of the thermoplastic tube V25d is respectively connected to the thermoplastic tube W25f and one end of the thermoplastic tube X25g through a T-type three-way connector B25e, the other end of the thermoplastic tube W25f is connected to the female Luer connector 25h, the female Luer connector 25h is connected to the needleless sampling connector 25i, the needleless sampling connector 25i is connected to the coreless Luer cap 25j, the coreless Luer cap 25j is connected to the ziplock bag A25k, and the other end of the thermoplastic tube X25g is connected to the plug G25m.

[0054] Specifically, as a preferred example of the first pH value measuring pipeline 26: Fig.17 As shown, it includes a single connector I26a, a medical silicone tube A26b, a sterile female connector A26c and a ziplock bag B26d; wherein one end of the medical silicone tube A26b is connected to the first pH value measuring port 12 through the single connector I26a, and the other end is connected to the ziplock bag B26d through the sterile female connector A26c.

[0055] Specifically, as a preferred example of the first DO value measuring pipeline 27: Fig.18 As shown, it specifically includes a single connector J27a, a medical silicone tube B27b, a sterile female connector B27c and a ziplock bag C27d; wherein one end of the medical silicone tube B27b is connected to the first DO value measuring port 13 through the single connector 27a, and the other end is connected to the ziplock bag C27d through the sterile female connector B27c.

[0056] Specifically, in this embodiment, a second pH value measuring port 14, a second DO value measuring port 15 and a temperature measuring port 16 are also provided on the rear side of the disposable 3D bag body 1, and a second pH value measuring pipeline 28, a second DO value measuring pipeline 29 and a temperature measuring pipeline 30 are connected in sequence on the side of the second pH value measuring port 14, the second DO value measuring port 15 and the temperature measuring port 16 located outside the disposable 3D bag body 1.

[0057] Specifically, as a preferred example of the second pH value measuring pipeline 28: Fig.19As shown, it specifically includes a single connector K28a, a medical silicone tube C28b, a sterile female connector C28c and a ziplock bag D28d; wherein one end of the medical silicone tube C28b is connected to the second pH value measuring port 14 through the single connector K28a, and the other end is connected to the ziplock bag D28d through the sterile female connector C28c.

[0058] Specifically, as a preferred example of the second DO value measuring pipeline 29: Fig. 20 As shown, it specifically includes a single connector L29a, a medical silicone tube D29b, a sterile female connector D29c and a ziplock bag E29d; wherein, one end of the medical silicone tube D29b is connected to the second DO value measuring port 15 through the single connector L29a, and the other end is connected to the ziplock bag E29d through the sterile female connector D29c.

[0059] Specifically, as a preferred example of the temperature measuring pipeline 30: Fig.21 As shown, it specifically includes a single joint M30a, a medical silicone tube E30b, a double joint 30c, a thermoplastic tube Y30d and a pearlescent tie 30e; wherein, one end of the medical silicone tube E30b is connected to the temperature measuring port 16 through the single joint M30a, and the other end is connected to one end of the thermoplastic tube Y30d through the double joint 30c, and the pearlescent tie 30e is passed through the inside of the thermoplastic tube X30d.

[0060] Specifically, in this embodiment, a stirring paddle kit 17 is provided at the bottom of the disposable 3D bag body 1, and the stirring paddle kit 17 is used to install a stirring paddle (not shown in the figure). Two first air inlets 17a, two second air inlets 17b and two first liquid outlets 17c are opened on the stirring paddle kit 17, and the two air inlets 17a are commonly connected to a first air inlet pipeline 31, the two second air inlets 17b are commonly connected to a second air inlet pipeline 32, and the two first liquid outlets 17c are commonly connected to a first liquid outlet pipeline 33.

[0061] Specifically, as a preferred example of the first air intake pipeline 31 and the second air intake pipeline 32: Fig. 22As shown, each of them comprises two thermoplastic tubes Z31a, a Y-type three-way joint B31b, a thermoplastic tube II31c, a reducer G31d, a thermoplastic tube III31e and an air filter C31f; wherein, one end of the two thermoplastic tubes Z31a in the first air intake pipeline 31 is respectively connected to the two first air intake ports 17a, and the other end is connected to one end of the corresponding thermoplastic tube II31c through the corresponding Y-type three-way joint B31b, and the other end of the corresponding thermoplastic tube II31c is connected to the corresponding thermoplastic tube II31c through the corresponding reducer G31d. One end of the two thermoplastic tubes Z31a in the second air intake pipeline 32 is connected to the two second air inlets 17b respectively, and the other ends are connected to one end of the corresponding thermoplastic tube II31c through the corresponding Y-type three-way joints B31b, the other end of the corresponding thermoplastic tube II31c is connected to one end of the corresponding thermoplastic tube III31e through the corresponding reducer G31d, and the other end of the corresponding thermoplastic tube III31e is connected to the corresponding air filter C31f.

[0062] Specifically, as a preferred example of the first liquid outlet pipeline 33: Fig.23 As shown, it specifically includes two thermoplastic tubes IV33a, a Y-type three-way connector C33b, a thermoplastic tube V33c, an equal-diameter connector I33d, a peristaltic pump tube E33e, an equal-diameter connector J33f, a thermoplastic tube VII33g, a sterile connector 33h and a ziplock bag F33i; wherein, one end of the two thermoplastic tubes IV33a are respectively connected to the two first liquid outlets 17c, and the other ends are connected to one end of the thermoplastic tube V33c through the Y-type three-way connector C33b, the other end of the thermoplastic tube V33c is connected to one end of the peristaltic pump tube E33e through the equal-diameter connector I33d, the other end of the peristaltic pump tube E33e is connected to one end of the thermoplastic tube VI33g through the equal-diameter connector J33f, and the other end of the thermoplastic tube VI33g is connected to the ziplock bag F33i through the sterile connector 33h.

[0063] Specifically, in this embodiment, if Figure 4 As shown, the disposable 3D bag body 1 is formed by an upper bag sheet 1a, a lower bag sheet 1b, a front bag sheet 1c and a rear bag sheet 1d.

[0064] More specifically, in order to ensure the firmness of the connection between the components in the pipeline, the joints of the components in the pipeline are fixed by cable ties. In order to facilitate the control of the inflow and outflow of liquid or gas in each pipeline, a corresponding thumb clamp or pinky clamp is also provided in the pipeline.

[0065] like Figure 5As shown, a second embodiment of a disposable 3D sterile bioreactor bag for high-density adherent cell culture provided by the utility model is provided. The difference between this embodiment and the first embodiment mentioned above is that the first liquid inlet 2 is opened on the retention membrane 3, and the first liquid inlet 2 is connected to the transfer port 34 arranged on the top of the disposable 3D bag body 1 through a connecting tube 35.

[0066] like Figure 6 and Figure 7 As shown, a third embodiment of a disposable 3D sterile bioreactor bag for high-density adherent cell culture provided by the utility model is shown. The difference between this embodiment and the first embodiment mentioned above is that: (1) the first liquid inlet 2 is arranged on the rear side of the disposable 3D bag body 1; (2) the interception membrane 3 and the plastic filter 4 are fully spread on the inner surface of the rear side of the disposable 3D bag body 1; (3) the sampling and liquid outlet 11, the first pH value measuring port 12, the first DO value measuring port 13, the second pH value measuring port 14, the second DO value measuring port 15 and the temperature measuring port 16 are all arranged on the front side of the disposable 3D bag body 1.

[0067] like Figure 8 As shown, it is a fourth embodiment of the disposable 3D sterile bioreactor bag for high-density adherent cell culture provided by the utility model. The difference between this embodiment and the above-mentioned third embodiment is that the first liquid inlet 2 is opened on the retention membrane 3, and the first liquid inlet 2 is connected to the transfer port 34 arranged on the front side of the disposable 3D bag body 1 through a connecting tube 35.

[0068] The first and second embodiments are suitable for the case where the disposable 3D bag body 1 is small in volume. In this case, since the disposable 3D bag body 1 is small in volume and there is not enough space on the front side surface, part of the pipeline is arranged on the rear side of the disposable 3D bag body 1.

[0069] The third and fourth embodiments are suitable for the case where the disposable 3D bag body 1 is relatively large. In this case, since the disposable 3D bag body 1 is relatively large, there is enough space on the front side surface, so no pipeline is set on the rear side of the disposable 3D bag body 1.

[0070] Of course, in actual applications, the specific positions of the various pipelines connected to the surface of the disposable 3D bag body 1 are not limited to the above four embodiments, but can be reasonably adjusted according to actual needs. For example, all pipelines are set on a side of the disposable 3D bag body 1 except the bottom side, such as the front side, back side, left side, right side or top side of the disposable 3D bag body 1; or they are dispersedly set on multiple sides of the disposable 3D bag body 1 except the bottom side.

[0071] In addition, the position and size of the retention membrane 3 and the plastic filter screen 4 are not limited to the above three embodiments, and can also be reasonably adjusted according to actual needs.

[0072] Finally, it should be noted that the above description is only an embodiment of the present utility model, and does not limit the patent scope of the present utility model. Any equivalent structure or equivalent process transformation made using the contents of the present utility model specification and drawings, or directly or indirectly used in other related technical fields, are also included in the patent protection scope of the present utility model.

Claims

1. A disposable 3D sterile bioreactor bag for high-density adherent cell culture, comprising a disposable 3D bag body (1), characterized in that: A first liquid inlet (2) is arranged on the front side or the rear side of the disposable 3D bag body (1), a retention membrane (3) is arranged on the side of the first liquid inlet (2) located on the inner surface of the disposable 3D bag body (1), and a plastic filter net (4) is arranged between the retention membrane (3) and the disposable 3D bag body (1), and the plastic filter net (4) is used to prevent the retention membrane (3) from sticking to the disposable 3D bag body (1).

2. The disposable 3D sterile bioreactor bag for high-density adherent cell culture according to claim 1, characterized in that: A first liquid inlet pipeline (18) is connected to a side of the first liquid inlet (2) located outside the disposable 3D bag body (1).

3. The disposable 3D sterile bioreactor bag for high-density adherent cell culture according to claim 2, characterized in that: The disposable 3D bag body (1) is also provided with a second liquid inlet (5), a third liquid inlet (6), a fourth liquid inlet (7), a fifth liquid inlet (8), an air outlet (9) and a pressure measuring port (10), and a second liquid inlet pipeline (19), a third liquid inlet pipeline (20), a fourth liquid inlet pipeline (21), a fifth liquid inlet pipeline (22), an air outlet pipeline (23) and a pressure measuring port (24) are sequentially connected to a side of the second liquid inlet (5), the third liquid inlet (6), the fourth liquid inlet (7), the fifth liquid inlet (8), the air outlet (9) and the pressure measuring port (10) located outside the disposable 3D bag body (1).

4. The disposable 3D sterile bioreactor bag for high-density adherent cell culture according to claim 2, characterized in that: The disposable 3D bag body (1) is also provided with a sampling and liquid outlet port (11), a first pH value measuring port (12) and a first DO value measuring port (13), and a sampling and liquid outlet pipeline (25), a first pH value measuring pipeline (26) and a first DO value measuring pipeline (27) are sequentially connected to the sampling and liquid outlet port (11), the first pH value measuring port (12) and the first DO value measuring port (13) on the side outside the disposable 3D bag body (1).

5. The disposable 3D sterile bioreactor bag for high-density adherent cell culture according to claim 2, characterized in that: The disposable 3D bag body (1) is also provided with a second pH value measuring port (14), a second DO value measuring port (15) and a temperature measuring port (16), and a second pH value measuring pipeline (28), a second DO value measuring pipeline (29) and a temperature measuring pipeline (30) are sequentially connected to the second pH value measuring port (14), the second DO value measuring port (15) and the temperature measuring port (16) on the side outside the disposable 3D bag body (1).

6. The disposable 3D sterile bioreactor bag for high-density adherent cell culture according to claim 1, characterized in that: A stirring paddle kit (17) is arranged on the bottom side of the disposable 3D bag body (1), and the stirring paddle kit (17) is used to install a stirring paddle. Two first air inlets (17a), two second air inlets (17b) and two first liquid outlets (17c) are provided on the stirring paddle kit (17), and the two air inlets (17a) are commonly connected to a first air inlet pipeline (31), the two second air inlets (17b) are commonly connected to a second air inlet pipeline (32), and the two first liquid outlets (17c) are commonly connected to a first liquid outlet pipeline (33).

7. The disposable 3D sterile bioreactor bag for high-density adherent cell culture according to any one of claims 1 to 6, characterized in that: The disposable 3D bag body (1) is formed by enclosing an upper bag sheet (1a), a lower bag sheet (1b), a front bag sheet (1c) and a rear bag sheet (1d).

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