Protein separation device with primary filtering structure

Abstract

The utility model provides a protein separation device with a primary filtering structure, which relates to the technical field of protein separation and comprises a filtering bin, and the top end of the filtering bin is communicated with a precipitation bin. Through the design of the primary filtering bin, large impurity particles can be effectively removed, so that the burden of a subsequent filter element is relieved, the efficiency of a protein separation process and the purity of a final product can be improved, meanwhile, the loss of the subsequent filter element can be reduced through primary filtering, the service life of the filter element is prolonged, and the production cost is reduced. The collection box is designed, so that the impurities are more conveniently collected and treated, a user can easily take down the collection box to clean or replace the collection box, the maintenance work of equipment is simplified, in conclusion, biological materials can be more effectively stirred through the stirring blades driven by the motor, the impurities are separated through centrifugal force, and the efficiency is improved. Such a physical process enhances the ability of the device to handle different types of biological materials.

Description

Technical Field

[0001] The utility model relates to the technical field of protein separation, in particular to a protein separation device with a primary filtering structure. Background Art

[0002] Protein separation refers to the technical means of extracting and separating total protein or specific component protein from mixed biological materials. It is a necessary technical prerequisite for achieving protein purification.

[0003] Announcement No. CN211245783U discloses a protein separation device, including a filter bin, a sedimentation bin, a connecting pipe, a baffle, a support rod, an orifice plate, a filter element, and a cylinder, wherein the sedimentation bin is connected to the filter bin through a connecting pipe, the sedimentation bin is located above the filter bin, the lower end of the connecting pipe extends into the filter bin, a baffle is fixedly connected to the lower end of the connecting pipe, a number of support rods are fixedly connected to the outer wall of the filter bin, a cylinder is fixedly connected to the inside of the filter bin, two orifice plates are fixedly connected to the inside of the cylinder, a filter element is sandwiched between the two orifice plates, and the filter element is located below the connecting pipe. Although this protein separation device can be equipped with manual valves on the connecting pipe and the discharge pipe to control the feeding and discharging process, it lacks an effective primary filtration structure to remove larger impurity particles, resulting in an increased burden on the subsequent filtration system, affecting the efficiency of protein separation and the purity of the product, and because large particle impurities cannot be effectively removed, the filter element in the prior art will be blocked and worn more quickly, and needs to be replaced frequently, thereby increasing the maintenance cost. Therefore, improvements are needed. Summary of the invention

[0004] The purpose of the utility model is to solve the technical problems raised in the above background technology.

[0005] The utility model adopts the following technical scheme: a protein separation device with a primary filtration structure, comprising a filtration bin, the top of the filtration bin is connected to a sedimentation bin, the top of the sedimentation bin is connected to a connecting valve, the top of the connecting valve is connected to a primary filtration bin, a motor is fixedly installed on the top of the primary filtration bin, a stirring blade is installed on the output end of the motor, a mounting frame is fixedly installed on the surface of the primary filtration bin, a plug plate is slidably connected to the inner wall of the mounting frame, a collecting box is fixedly installed on the side of the plug plate, coarse holes are opened on one side of the collecting box, and fine holes are opened on the other side of the collecting box.

[0006] Preferably, the coarse holes are evenly distributed on one side of the collection box, and the fine holes are evenly distributed on the other side of the collection box.

[0007] Preferably, the top surfaces of the plug plate and the mounting frame are both provided with screw holes, and the inner walls of the screw holes are provided with bolts.

[0008] Preferably, the bottom end of the filter bin is connected to a discharge pipe, and a filter element is fixedly mounted on the inner wall of the filter bin.

[0009] Preferably, a support foot is fixedly installed at the bottom end of the filter bin, and a support plate is fixedly installed at the bottom end of the support foot. Here, the filter bin can be stably supported.

[0010] Preferably, a vibration mechanism is provided on the surface of the sedimentation bin, the vibration mechanism comprises a motor, the motor is fixedly mounted on the surface of the sedimentation bin, an impact block is installed at the output end of the motor, and a short rod is fixedly mounted on the surface of the sedimentation bin. Here, the mixing effect can be improved.

[0011] Preferably, the impact block and the short rod interfere with each other.

[0012] Compared with the prior art, the advantages and positive effects of the utility model are:

[0013] 1. The utility model can effectively remove larger impurity particles through the design of the primary filter bin, which not only reduces the burden of the subsequent filter element, but also improves the efficiency of the protein separation process and the purity of the final product. At the same time, the preliminary filtration can reduce the loss of the subsequent filter element and extend the service life of the filter element, thereby reducing the frequency and cost of replacing the filter element. The design of the collection box makes it more convenient to collect and process impurities. The user can easily remove the collection box for cleaning or replacement, which simplifies the maintenance of the equipment. In summary, the stirring blade driven by the motor can more effectively stir the biological material and separate the impurities by centrifugal force. Such a physical process enhances the ability of the equipment to process different types of biological materials.

[0014] 2. The utility model can mix the protein denaturant with the biological material more evenly and quickly by means of mechanical vibration. This improvement in efficiency helps to shorten the time of the entire protein denaturation process, and the mechanical vibration can promote the protein denaturant to contact the biological material more deeply and comprehensively, thereby improving the efficiency and quality of protein denaturation and ensuring the stability and consistency of the protein in the treated biological material. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 A schematic diagram of a protein separation device with a primary filtration structure is provided for the utility model;

[0016] Figure 2 A cross-sectional view of a protein separation device with a primary filtration structure is provided for the utility model;

[0017] Figure 3 The utility model proposes a protein separation device with a primary filtration structure Figure 2 Enlarged view of point A in the middle;

[0018] Figure 4 The utility model provides an exploded view of an insert plate in a protein separation device with a primary filtration structure;

[0019] Figure 5 The utility model proposes a protein separation device with a primary filtration structure Figure 4 Enlarged view of point B in the middle;

[0020] Figure 6 The utility model proposes a protein separation device with a primary filtration structure Figure 4 Enlarged view of center C.

[0021] Legend:

[0022] 1. Filter chamber; 2. Sedimentation chamber; 3. Connecting valve; 4. Primary filter chamber; 5. Motor; 6. Stirring blade; 7. Mounting frame; 8. Insert plate; 9. Collecting box; 10. Coarse hole; 11. Fine hole; 12. Screw hole; 13. Bolt; 14. Discharge pipe; 15. Filter element; 16. Support foot; 17. Support plate; 18. Motor; 19. Impact block; 20. Short rod. DETAILED DESCRIPTION

[0023] In order to more clearly understand the above-mentioned purpose, features and advantages of the utility model, the utility model is further described below in conjunction with the accompanying drawings and embodiments. It should be noted that the embodiments of the present application and the features in the embodiments can be combined with each other without conflict.

[0024] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways than those described herein. Therefore, the present invention is not limited to the specific embodiments of the following disclosure. Example

[0025] See also Figure 1-6 The utility model provides a technical solution: a protein separation device with a primary filtration structure, comprising a filter bin 1, the bottom end of the filter bin 1 is connected to a discharge pipe 14, which is fixed by a threaded connection or a flange connection, so that the discharge pipe 14 can be firmly connected to the filter bin 1, so as to facilitate the discharge of materials after protein separation. A filter element 15 is fixedly installed on the inner wall of the filter bin 1. The filter element 15 can be made of stainless steel or a polymer material to meet different protein separation requirements. The fixing method can be a snap-on type or a threaded tightening method, which not only ensures the stability of the filter element 15, but also facilitates replacement and cleaning. A support foot 16 is fixedly installed at the bottom end of the filter bin 1, and a support plate 17 is fixedly installed at the bottom end of the support foot 16. The support foot 16 and the support plate 17 are usually made of thickened stainless steel to ensure the stability and durability of the entire device.

[0026] See also Figure 1-6The top of the filter bin 1 is connected to the sedimentation bin 2, the top of the sedimentation bin 2 is connected to the connecting valve 3, the top of the connecting valve 3 is connected to the primary filter bin 4, the top of the primary filter bin 4 is fixedly installed with a motor 5, the output end of the motor 5 is installed with a stirring blade 6, which is connected to the motor shaft by a keyway connection method to ensure the stability of the stirring blade 6 when rotating at high speed. The surface of the primary filter bin 4 is fixedly installed with a mounting frame 7, and the inner wall of the mounting frame 7 is slidably connected with a plug-in plate 8, which is connected to the mounting frame 7 by a guide rail sliding method. This method not only ensures the smooth movement of the plug-in plate 8, but also facilitates the user to quickly disassemble. The top surfaces of the plug-in plate 8 and the mounting frame 7 are both provided with screw holes 12, and the inner walls of the screw holes 12 are inserted with bolts 13. By tightening the bolts 13, the position of the plug-in plate 8 can be conveniently fixed or adjusted to accommodate collection boxes 9 of different sizes. A collecting box 9 is fixedly installed on the side of the plug plate 8. Coarse holes 10 are opened on one side of the collecting box 9, and fine holes 11 are opened on the other side of the collecting box 9. The design of the coarse holes 10 and the fine holes 11 not only facilitates the collection and filtration of large particles of impurities, but also ensures the collection efficiency and filtration effect. The coarse holes 10 are evenly distributed on one side of the collecting box 9, and the fine holes 11 are evenly distributed on the other side of the collecting box 9. Example

[0027] See also Figure 4 and Figure 6 A vibration mechanism is provided on the surface of the sedimentation bin 2, and the vibration mechanism includes a motor 18. The motor 18 is fixedly mounted on the surface of the sedimentation bin 2 and can be fixed by bolts or welding to ensure stability during the vibration process. An impact block 19 is installed at the output end of the motor 18, and the impact block 19 can be made of cast iron or high-density polyethylene to improve impact resistance. A short rod 20 is fixedly installed on the surface of the sedimentation bin 2, and the short rod 20 interferes with the impact block 19. The short rod 20 can be made of stainless steel to improve its corrosion resistance and durability.

[0028] Working principle: connect the primary filter bin 4 to the external biological material feed port, and then inject the external biological material into the primary filter bin 4, and then the motor 5 can drive the stirring blade 6 to rotate, and the stirring blade 6 can stir the biological material at this time, so that due to the effect of centrifugal force, heavier impurities will flow to the inner wall of the primary filter bin 4, and then enter the collecting box 9 through the coarse hole 10, and be blocked by the fine hole 11, so that large particles of impurities can be collected by the collecting box 9, thereby performing preliminary filtration of the biological material and reducing the loss of the subsequent filter element 15, and then open the connecting valve 3, and then the biological material enters the precipitation bin 2 for protein denaturation, and finally enters the filtering bin 1, passes through the filter element 15, and the protein is intercepted here, and can be directly collected later, and the set collecting box 9 can also be conveniently removed. When removing the collecting box 9, you only need to unscrew the bolt 13, and then pull the plug plate 8 outward until the plug plate 8 leaves the mounting frame 7, then you can remove the collecting box 9, the utility model can effectively remove larger impurity particles through the design of the primary filter bin 4, which not only reduces the subsequent The burden of the subsequent filter element 15 can also improve the efficiency of the protein separation process and the purity of the final product. At the same time, the preliminary filtration can reduce the loss of the subsequent filter element 15 and extend the service life of the filter element 15, thereby reducing the frequency and cost of replacing the filter element 15. The design of the collection box 9 makes it more convenient to collect and process impurities. The user can easily remove the collection box 9 for cleaning or replacement, which simplifies the maintenance of the equipment. When the biological material is in the precipitation bin 2, the motor 18 can drive the impact block 19 to move, and the impact block 19 can then hit the short rod 20, so that the precipitation bin 2 is vibrated back and forth, so that the protein denaturant and the biological material are fully mixed, thereby improving the protein denaturation efficiency. The utility model can mix the protein denaturant and the biological material more evenly and quickly through mechanical vibration. This improvement in efficiency helps to shorten the time of the entire protein denaturation process, and mechanical vibration can promote the protein denaturant to contact the biological material more deeply and comprehensively, thereby improving the efficiency and quality of protein denaturation.

[0029] The above description is only a preferred embodiment of the present invention and does not limit the present invention in other forms. Any technician familiar with the profession may use the technical content disclosed above to change or modify it into an equivalent embodiment with equivalent changes and apply it to other fields. However, any simple modification, equivalent change and modification made to the above embodiment based on the technical essence of the present invention without departing from the content of the technical solution of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A protein separation device with a primary filtration structure, comprising a filtration chamber (1), characterized in that: The top of the filtration bin (1) is connected to a sedimentation bin (2), the top of the sedimentation bin (2) is connected to a communication valve (3), the top of the communication valve (3) is connected to a primary filtration bin (4), a motor (5) is fixedly mounted on the top of the primary filtration bin (4), an agitator (6) is mounted on the output end of the motor (5), a mounting frame (7) is fixedly mounted on the surface of the primary filtration bin (4), a plug plate (8) is slidably connected to the inner wall of the mounting frame (7), a collecting box (9) is fixedly mounted on the side of the plug plate (8), one side of the collecting box (9) is provided with a coarse hole (10), and the other side of the collecting box (9) is provided with a fine hole (11).

2. A protein separation device with a primary filtration structure according to claim 1, characterized in that: The coarse holes (10) are evenly distributed on one side of the collection box (9), and the fine holes (11) are evenly distributed on the other side of the collection box (9).

3. A protein separation device with a primary filtration structure according to claim 1, characterized in that: The top surfaces of the insert plate (8) and the mounting frame (7) are both provided with screw holes (12), and the inner walls of the screw holes (12) are provided with bolts (13).

4. A protein separation device with a primary filtration structure according to claim 1, characterized in that: The bottom end of the filter bin (1) is connected to a discharge pipe (14), and a filter element (15) is fixedly mounted on the inner wall of the filter bin (1).

5. The protein separation device with a primary filtration structure according to claim 1, characterized in that: A support foot (16) is fixedly mounted on the bottom end of the filter bin (1), and a support plate (17) is fixedly mounted on the bottom end of the support foot (16).

6. The protein separation device with a primary filtration structure according to claim 1, characterized in that: A vibration mechanism is provided on the surface of the sedimentation bin (2), the vibration mechanism comprising a motor (18), the motor (18) being fixedly mounted on the surface of the sedimentation bin (2), an impact block (19) being mounted on the output end of the motor (18), and a short rod (20) being fixedly mounted on the surface of the sedimentation bin (2).

7. A protein separation device with a primary filtration structure according to claim 6, characterized in that: The impact block (19) and the short rod (20) interfere with each other.

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